##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib42_TAATCG_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2140450 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23009834380621 33.0 33.0 33.0 27.0 33.0 2 31.591070569272816 33.0 33.0 33.0 27.0 33.0 3 31.806949940433086 33.0 33.0 33.0 27.0 33.0 4 35.6479871055152 37.0 37.0 37.0 33.0 37.0 5 35.16921862225233 37.0 37.0 37.0 33.0 37.0 6 34.983670723446004 37.0 37.0 37.0 33.0 37.0 7 34.7941049779252 37.0 33.0 37.0 27.0 37.0 8 34.83991216800206 37.0 33.0 37.0 27.0 37.0 9 34.72924712093251 37.0 33.0 37.0 27.0 37.0 10-11 34.582074096568476 37.0 33.0 37.0 27.0 37.0 12-13 34.586296339554764 37.0 33.0 37.0 27.0 37.0 14-15 35.53222289705435 37.0 33.0 40.0 27.0 40.0 16-17 35.43504169683945 37.0 33.0 40.0 27.0 40.0 18-19 35.64988974281109 37.0 33.0 40.0 27.0 40.0 20-21 35.319965427830596 37.0 33.0 40.0 27.0 40.0 22-23 35.351327524585955 37.0 33.0 40.0 27.0 40.0 24-25 35.19192763203999 37.0 33.0 40.0 27.0 40.0 26-27 34.81401247401247 37.0 33.0 40.0 27.0 40.0 28-29 34.451605503515616 37.0 33.0 40.0 27.0 40.0 30-31 34.34708846270644 37.0 33.0 40.0 27.0 40.0 32-33 34.33149688149688 37.0 33.0 40.0 24.5 40.0 34-35 34.18526735032353 37.0 33.0 40.0 24.5 40.0 36-37 33.86925623116635 37.0 33.0 37.0 22.0 40.0 38-39 33.775066457987805 37.0 33.0 37.0 22.0 40.0 40-41 33.48396528767315 37.0 33.0 37.0 22.0 40.0 42-43 33.100531663902444 37.0 33.0 37.0 22.0 40.0 44-45 32.97649933425214 37.0 33.0 37.0 22.0 40.0 46-47 32.83156719381438 37.0 33.0 37.0 22.0 40.0 48-49 32.848472050269805 37.0 33.0 37.0 22.0 40.0 50-51 33.13571001424934 37.0 33.0 37.0 22.0 40.0 52-53 32.99706089840922 37.0 33.0 37.0 22.0 40.0 54-55 32.63834427340045 37.0 33.0 37.0 22.0 40.0 56-57 32.68490294097036 37.0 33.0 37.0 22.0 40.0 58-59 32.397574108248264 37.0 33.0 37.0 22.0 40.0 60-61 32.309115372935594 37.0 30.0 37.0 22.0 40.0 62-63 32.271412319839285 35.0 30.0 37.0 22.0 40.0 64-65 31.925128594454435 33.0 27.0 37.0 22.0 40.0 66-67 31.57540447102245 33.0 27.0 37.0 18.5 40.0 68-69 31.389750986941998 33.0 27.0 37.0 15.0 40.0 70-71 31.226290966852766 33.0 27.0 37.0 15.0 37.0 72-73 31.006894578242893 33.0 27.0 37.0 15.0 37.0 74-75 30.649317199654277 33.0 27.0 37.0 15.0 37.0 76-77 29.576315961596862 33.0 27.0 35.0 15.0 37.0 78-79 30.516984979793968 33.0 27.0 37.0 15.0 37.0 80-81 30.230815949917073 33.0 27.0 37.0 15.0 37.0 82-83 30.21721039968231 33.0 27.0 37.0 15.0 37.0 84-85 30.092416314326428 33.0 27.0 37.0 15.0 37.0 86-87 29.890799598215327 33.0 27.0 37.0 15.0 37.0 88-89 29.773082529374662 33.0 27.0 37.0 15.0 37.0 90-91 29.689403630077788 33.0 27.0 37.0 15.0 37.0 92-93 29.478849073792894 33.0 27.0 35.0 15.0 37.0 94-95 29.38428064192109 33.0 27.0 33.0 15.0 37.0 96-97 29.252589642364924 33.0 27.0 33.0 15.0 37.0 98-99 28.978316008316007 33.0 27.0 33.0 15.0 37.0 100-101 28.17738559648672 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.2574313402422774 2306 2 0.5463264102593826 2306 3 0.4769180014448118 2306 4 0.3607365660512798 2306 5 0.6341063156707918 2306 6 0.7028175775322012 2306 7 0.8637406768231131 2306 8 0.712604356305782 2306 9 1.0498786462632168 2306 10-11 1.2270435099983104 2306 12-13 1.3937208599238602 2306 14-15 1.507667041201934 2306 16-17 1.469066229090842 2306 18-19 1.2865892282651856 2306 20-21 1.5863168135375005 2306 22-23 1.2690478330309034 2306 24-25 1.1772876252358415 2306 26-27 1.3678205558692582 2306 28-29 1.6629492242837784 2306 30-31 1.5249739239358604 2306 32-33 1.2204385219435494 2306 34-35 1.429142262027284 2306 36-37 1.4861215031688175 2306 38-39 1.120866681784932 2306 40-41 1.3014278527594598 2306 42-43 1.2270196244315343 2306 44-45 1.2431131469336805 2306 46-47 1.214213338006914 2306 48-49 1.1664584950672463 2306 50-51 1.293745602226572 2306 52-53 1.297365987060303 2306 54-55 1.7285105735768553 2306 56-57 1.4180196078483362 2306 58-59 1.3007541938590066 2306 60-61 1.0833837928515777 2306 62-63 0.8346347161358239 2306 64-65 0.8900178985398881 2306 66-67 1.115918388640921 2306 68-69 0.9225629553111325 2306 70-71 0.8961612700456634 2306 72-73 0.7192520036873766 2306 74-75 0.8688789370682599 2306 76-77 0.9901018325500957 2306 78-79 1.0073276577579975 2306 80-81 1.5643693252144786 2306 82-83 1.1945861810701643 2306 84-85 1.04711451752107 2306 86-87 0.9103554857207925 2306 88-89 0.8140304869168951 2306 90-91 0.4766895212339719 2306 92-93 0.18552666698166576 2306 94-95 0.3183369270997005 2306 96-97 0.3779899149157835 2306 98-99 0.5881755997532707 2306 100-101 0.3595353354594728 2307 1 0.7456092133316901 2307 2 0.4960537942584935 2307 3 0.3532120423321494 2307 4 0.2770500289320026 2307 5 0.5154450170272966 2307 6 0.5448783319552604 2307 7 0.753943697347708 2307 8 0.7224498907646506 2307 9 0.9295917672359337 2307 10-11 1.1156684935604204 2307 12-13 1.3544439245525552 2307 14-15 1.3129625690856415 2307 16-17 1.3216532993033638 2307 18-19 1.1864374939418667 2307 20-21 1.4624570969772108 2307 22-23 1.1407771996918399 2307 24-25 1.1914611283767442 2307 26-27 1.2386147333174904 2307 28-29 1.5060473356972324 2307 30-31 1.471715176452122 2307 32-33 1.2124967474612163 2307 34-35 1.267065727234602 2307 36-37 1.262077176515163 2307 38-39 0.9470079767867929 2307 40-41 1.0666316319556728 2307 42-43 1.1153588416847384 2307 44-45 0.8743370359855405 2307 46-47 0.904085071925607 2307 48-49 0.9287671833746032 2307 50-51 0.8274417491863204 2307 52-53 1.1472228510153144 2307 54-55 1.4870274211445462 2307 56-57 1.1719591366626858 2307 58-59 1.0574542757955498 2307 60-61 0.9043893867902995 2307 62-63 0.8082921202650226 2307 64-65 0.8974726058443849 2307 66-67 0.8628700460855185 2307 68-69 0.7606730537361308 2307 70-71 0.6138524805820929 2307 72-73 0.613755174934731 2307 74-75 0.9060477769076272 2307 76-77 1.0026998276339043 2307 78-79 0.9054906608837214 2307 80-81 1.5632763323055272 2307 82-83 1.2945214410922148 2307 84-85 1.1098273249095172 2307 86-87 0.9533569929484003 2307 88-89 0.8303393977679221 2307 90-91 0.670873544621287 2307 92-93 0.7430486431694945 2307 94-95 0.6894664374024657 2307 96-97 0.5940828572466224 2307 98-99 0.5206821496182314 2307 100-101 0.5891963609811874 1101 1 1.0970028780730452 1101 2 0.5906250405362314 1101 3 0.42500272072813416 1101 4 0.31508564286900054 1101 5 0.5838653505409468 1101 6 0.5523551240799307 1101 7 0.6889814694900807 1101 8 0.7308044325573704 1101 9 0.910542853685925 1101 10-11 1.0298305560587693 1101 12-13 1.164193399005093 1101 14-15 1.3110954881944394 1101 16-17 1.3678572413375463 1101 18-19 1.0533801678475356 1101 20-21 1.2808764606278658 1101 22-23 1.0220416275866882 1101 24-25 1.1244650978131006 1101 26-27 1.178076003281646 1101 28-29 1.3415022009700124 1101 30-31 1.392612346089109 1101 32-33 0.8428360916426954 1101 34-35 0.9729730350150874 1101 36-37 1.2162267377160276 1101 38-39 0.8669378854976202 1101 40-41 1.03782812023789 1101 42-43 1.203860803599305 1101 44-45 0.8301888250232139 1101 46-47 0.8372852375498212 1101 48-49 0.8016062221675071 1101 50-51 0.8486897679454586 1101 52-53 1.0358043748129617 1101 54-55 1.417114870615876 1101 56-57 1.0280479791411352 1101 58-59 1.075323623043687 1101 60-61 0.9807284624379946 1101 62-63 0.6547778051344864 1101 64-65 0.546825215196904 1101 66-67 0.7688249859667877 1101 68-69 0.6312272372378054 1101 70-71 0.7591723522800997 1101 72-73 0.5476003704179533 1101 74-75 0.7976697975476341 1101 76-77 0.6230312501636597 1101 78-79 0.6712161851135967 1101 80-81 1.2616127475953753 1101 82-83 1.0259943611453224 1101 84-85 0.9856910825296836 1101 86-87 0.6768812233904988 1101 88-89 0.6759693559559459 1101 90-91 0.4638132589781847 1101 92-93 0.5419368821187547 1101 94-95 0.5518518906686438 1101 96-97 0.5229958499382086 1101 98-99 0.6708439085731186 1101 100-101 0.6398952631814332 2201 1 1.5227768661089556 2201 2 0.860687448492655 2201 3 0.36738897625313527 2201 4 0.26698226767383915 2201 5 0.3952770026470276 2201 6 0.5490923170823692 2201 7 0.7101682475712181 2201 8 0.6771908205560209 2201 9 0.6906565570607412 2201 10-11 0.8789322488862013 2201 12-13 1.145091246332072 2201 14-15 1.2617603701201432 2201 16-17 1.1439280442160324 2201 18-19 1.0242404674714862 2201 20-21 1.2790038407159798 2201 22-23 1.0688593412201115 2201 24-25 1.0109869043820296 2201 26-27 1.1382235207944973 2201 28-29 1.3946209428706027 2201 30-31 1.4545238360579162 2201 32-33 1.0510748091643194 2201 34-35 0.9674697792844711 2201 36-37 1.0685268115057553 2201 38-39 0.8136645115819192 2201 40-41 1.128072698527319 2201 42-43 1.0865168097903108 2201 44-45 0.9644076271802504 2201 46-47 1.000886457546386 2201 48-49 1.05649008018149 2201 50-51 1.0198504444283572 2201 52-53 1.0954606914592944 2201 54-55 1.4847702847258901 2201 56-57 1.0820431438528146 2201 58-59 1.185558952003639 2201 60-61 1.0197703538948986 2201 62-63 0.9265232003050556 2201 64-65 0.8642722096532651 2201 66-67 0.811998268413717 2201 68-69 0.9871659762744791 2201 70-71 1.0099466441000224 2201 72-73 0.8464495889544494 2201 74-75 1.0131566822986535 2201 76-77 1.115382659876314 2201 78-79 1.0346971781227516 2201 80-81 1.4208519151938468 2201 82-83 1.2551289865415214 2201 84-85 1.1606286161990802 2201 86-87 1.006007438139342 2201 88-89 1.045872867008086 2201 90-91 0.9345641487079241 2201 92-93 1.1093454091809107 2201 94-95 1.0296056807612857 2201 96-97 0.7904749501943193 2201 98-99 0.9725307794572906 2201 100-101 1.0835170976031847 2304 1 -2.0520475581318394 2304 2 -0.37547091242621633 2304 3 -0.09364790258434752 2304 4 0.06151984295979673 2304 5 0.45320748952671863 2304 6 0.5713366497481189 2304 7 0.5094699695431331 2304 8 0.6685143653933991 2304 9 0.8373694056203931 2304 10-11 0.8675948055717413 2304 12-13 1.1454639865336134 2304 14-15 1.1758610429064476 2304 16-17 1.2459068238821729 2304 18-19 0.9070624315781615 2304 20-21 1.1942103236633628 2304 22-23 0.8759137483607233 2304 24-25 1.1229374601991395 2304 26-27 1.0801177325880218 2304 28-29 1.6254882468709724 2304 30-31 1.604781589078506 2304 32-33 1.0661952423040475 2304 34-35 0.7827197445230354 2304 36-37 1.2454808617622604 2304 38-39 0.9894069427359682 2304 40-41 0.9571656445165786 2304 42-43 1.0456428157375655 2304 44-45 1.0605781972922372 2304 46-47 1.0438925170140578 2304 48-49 0.9988417098221447 2304 50-51 0.953055638153252 2304 52-53 1.2129579532336692 2304 54-55 1.471486566739955 2304 56-57 1.2298798846203027 2304 58-59 0.9197208781044779 2304 60-61 0.6636642022179373 2304 62-63 0.46404420963088455 2304 64-65 0.5142289713020034 2304 66-67 0.5747040622308148 2304 68-69 0.4963932782745424 2304 70-71 0.49822411112544174 2304 72-73 0.38649510204548676 2304 74-75 0.8604318123796162 2304 76-77 0.7325246231535978 2304 78-79 0.8667869565521187 2304 80-81 1.4854690087960307 2304 82-83 1.0772329012893955 2304 84-85 0.8477916741811562 2304 86-87 0.6475754412715311 2304 88-89 0.6279683780256278 2304 90-91 0.5696013007581158 2304 92-93 0.3921772223619584 2304 94-95 0.2411769709839291 2304 96-97 0.3152146967895284 2304 98-99 0.7680005101720084 2304 100-101 0.538035596787207 1102 1 1.4734848396753364 1102 2 0.11528781692081225 1102 3 0.20699035904010188 1102 4 0.18483338044038788 1102 5 0.15776446574242442 1102 6 0.03843389093814409 1102 7 -4.6481686819605805 1102 8 -1.1777997569060972 1102 9 0.08733081806571619 1102 10-11 0.5402149198626987 1102 12-13 0.8736253634148738 1102 14-15 0.7932803141525255 1102 16-17 0.4898233129586558 1102 18-19 0.7247567720333308 1102 20-21 0.9600284598968969 1102 22-23 0.6069326406618174 1102 24-25 0.8954965454076387 1102 26-27 0.9765154980717767 1102 28-29 1.0024765068663228 1102 30-31 1.0737045600358144 1102 32-33 0.764078780508072 1102 34-35 1.016527674866083 1102 36-37 1.2775559900054816 1102 38-39 0.7343998796257836 1102 40-41 1.1009720727751997 1102 42-43 1.1839400939826845 1102 44-45 1.1032313891940149 1102 46-47 0.974245294241129 1102 48-49 0.8228161920586103 1102 50-51 0.61570987792458 1102 52-53 0.8079554736899013 1102 54-55 1.2144717427682323 1102 56-57 0.9752894152354941 1102 58-59 0.9569447182274757 1102 60-61 0.8369117957075076 1102 62-63 0.65084152266693 1102 64-65 0.591814047541078 1102 66-67 0.495598815814418 1102 68-69 0.5903863142908818 1102 70-71 0.6293569091939375 1102 72-73 0.4558814964188542 1102 74-75 0.43204140904234123 1102 76-77 0.4225871059997388 1102 78-79 0.5509736040708937 1102 80-81 1.04149679211152 1102 82-83 0.9770834590762973 1102 84-85 0.6444955583947483 1102 86-87 0.5670692043077779 1102 88-89 0.4409068541388983 1102 90-91 0.06977877589779169 1102 92-93 0.11285213042831899 1102 94-95 0.17175002171185838 1102 96-97 0.18132820384186843 1102 98-99 0.20624806638558368 1102 100-101 0.3497994304600738 2202 1 1.5749509991849138 2202 2 0.8855480068376451 2202 3 0.3149618058252486 2202 4 -0.2840383765592591 2202 5 0.06787992558992784 2202 6 0.456993227655353 2202 7 0.5479214204788718 2202 8 -3.768887281230061 2202 9 -0.47104949306234545 2202 10-11 0.5035462765540331 2202 12-13 0.11732798807852873 2202 14-15 0.14270810344769558 2202 16-17 0.26341589661572584 2202 18-19 0.7840588589491873 2202 20-21 1.1799415248458374 2202 22-23 -2.732817934941245 2202 24-25 -2.940297621660939 2202 26-27 -1.2702738349651668 2202 28-29 0.5061402875761587 2202 30-31 1.2659922118111524 2202 32-33 1.0917364656914188 2202 34-35 -2.0599052473748216 2202 36-37 -0.32454177246107463 2202 38-39 0.2618909004858665 2202 40-41 1.209048265963574 2202 42-43 1.2193346698895624 2202 44-45 1.2429547590018686 2202 46-47 0.713196724717676 2202 48-49 0.9466017419500758 2202 50-51 -3.7652420407237663 2202 52-53 -0.3639622387855326 2202 54-55 1.4012748267463593 2202 56-57 1.2860057087840104 2202 58-59 1.4498687564780681 2202 60-61 1.3759988683126068 2202 62-63 1.114558871342041 2202 64-65 1.1180068602267568 2202 66-67 1.1667092816837155 2202 68-69 1.3025394413318274 2202 70-71 1.2948739320095335 2202 72-73 1.2525940588231208 2202 74-75 0.7264295758530679 2202 76-77 1.2383403773348078 2202 78-79 1.3282020707806907 2202 80-81 1.7797221395656777 2202 82-83 1.7148580919821867 2202 84-85 1.5076078924399532 2202 86-87 1.3869671003706259 2202 88-89 1.282352900553608 2202 90-91 1.240177963101413 2202 92-93 1.198308642853675 2202 94-95 1.3911465336417521 2202 96-97 1.180035352017633 2202 98-99 1.3823417605553985 2202 100-101 1.2536156737258572 2305 1 1.3447980009693765 2305 2 0.7819321498077514 2305 3 0.1594816792248892 2305 4 -0.07295197748655369 2305 5 0.2858620597942334 2305 6 0.35572780498552703 2305 7 0.5988586896044197 2305 8 0.09452559764324775 2305 9 0.5170942814517829 2305 10-11 0.30563415903248625 2305 12-13 0.7954492740112187 2305 14-15 0.5824368428364437 2305 16-17 0.6949108114830693 2305 18-19 0.8079701054269037 2305 20-21 0.927679980354263 2305 22-23 0.5650131010097326 2305 24-25 0.6749186408536971 2305 26-27 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1208 46-47 -0.1412934215829651 1208 48-49 -1.4715843355303235 1208 50-51 -0.16937689575415504 1208 52-53 -1.4036913160773992 1208 54-55 -1.0485164206456048 1208 56-57 -6.202687732585179 1208 58-59 -2.1132481763008073 1208 60-61 -1.4049948103872012 1208 62-63 0.18870315946605132 1208 64-65 -0.12662916391845513 1208 66-67 0.6535646478048811 1208 68-69 0.34318544225759595 1208 70-71 -4.067213581543491 1208 72-73 -0.592806791282765 1208 74-75 -5.316445823377876 1208 76-77 -1.0306303349765074 1208 78-79 -3.835422720717247 1208 80-81 0.9193347438323727 1208 82-83 0.5245726751315232 1208 84-85 -2.6466180207567938 1208 86-87 -0.956267301357645 1208 88-89 -4.597645198421219 1208 90-91 -0.2394338550741395 1208 92-93 -1.949473906800037 1208 94-95 -4.64687976376878 1208 96-97 -3.293787969771749 1208 98-99 -6.17976092674882 1208 100-101 -3.835087116177352 1209 1 1.7836154239095947 1209 2 1.3430367004922914 1209 3 0.7997661144769701 1209 4 0.3843879752783792 1209 5 0.16099598566218987 1209 6 1.065680476748362 1209 7 0.5918491261328711 1209 8 0.24504662749823325 1209 9 -0.18557922824445683 1209 10-11 -3.3752673716819572 1209 12-13 -0.31746886583999157 1209 14-15 -1.6981361036724039 1209 16-17 -0.5369684362960925 1209 18-19 -16.776846311322444 1209 20-21 -14.24080545782789 1209 22-23 -3.7270943564484256 1209 24-25 -2.0220603991656816 1209 26-27 -1.4566633328106633 1209 28-29 -2.5043037016080376 1209 30-31 -0.025497980152820787 1209 32-33 -0.4400860417126964 1209 34-35 -3.781670675451366 1209 36-37 -3.6260323509667742 1209 38-39 -1.3787578269635645 1209 40-41 -10.206651586230233 1209 42-43 -5.29973472171217 1209 44-45 -2.690652098616411 1209 46-47 -3.3084873455298407 1209 48-49 -3.784277787720683 1209 50-51 -2.747450997815797 1209 52-53 -1.152839592778598 1209 54-55 -2.5411332177390733 1209 56-57 -0.5608832745928538 1209 58-59 -2.188139522877478 1209 60-61 -1.5406562941361486 1209 62-63 -0.795194680700277 1209 64-65 -0.2736096020329697 1209 66-67 0.6103018004447947 1209 68-69 -1.2239432682888278 1209 70-71 -0.9512500668934258 1209 72-73 -0.4004203223695981 1209 74-75 0.7402732915129775 1209 76-77 -16.5412176398166 1209 78-79 -16.52431477599601 1209 80-81 -21.7137646324244 1209 82-83 -9.65970267697815 1209 84-85 -3.110099583829143 1209 86-87 -2.4402910855706637 1209 88-89 -1.4089015009601624 1209 90-91 -5.286899284500649 1209 92-93 -2.666431205364276 1209 94-95 -0.7240695891975264 1209 96-97 0.2696035112875492 1209 98-99 1.360000569381068 1209 100-101 0.9639707583140726 1212 1 -28.61106760267527 1212 2 -16.232659222048508 1212 3 -5.954250829724021 1212 4 -1.5420549945475912 1212 5 -0.5192056962375631 1212 6 -2.581037105990255 1212 7 -0.5050009635962667 1212 8 -1.286695056905586 1212 9 0.04209021376547639 1212 10-11 0.6928967804819735 1212 12-13 0.2887884376949188 1212 14-15 0.06876505863300508 1212 16-17 -0.10337843587874573 1212 18-19 -0.7689324095654229 1212 20-21 -0.5811280132304191 1212 22-23 -0.6115172206861459 1212 24-25 0.2768054661988728 1212 26-27 -0.49354227150270447 1212 28-29 0.39579698283752407 1212 30-31 -0.5685940529377689 1212 32-33 0.2491641982605941 1212 34-35 -1.17530773150078 1212 36-37 -1.6802923985440898 1212 38-39 -0.431879305616377 1212 40-41 -0.22770663401621505 1212 42-43 0.17490114490441755 1212 44-45 0.9646067073599696 1212 46-47 -2.5081477311564804 1212 48-49 -1.0920135353939813 1212 50-51 0.3537433909826717 1212 52-53 -0.8686746998275581 1212 54-55 -0.14028392096514963 1212 56-57 -1.214878308189114 1212 58-59 0.0676772209979859 1212 60-61 0.2434290424524761 1212 62-63 -4.56484510221858 1212 64-65 -4.181574628534364 1212 66-67 -1.45101920034816 1212 68-69 0.5150557822516326 1212 70-71 -0.37440383760416296 1212 72-73 -0.9052615226533902 1212 74-75 -0.2139270235823858 1212 76-77 1.0228239277306628 1212 78-79 -1.0849993258895871 1212 80-81 0.844576265284374 1212 82-83 0.8831508141115378 1212 84-85 -3.4373970672396865 1212 86-87 -2.0339589208029167 1212 88-89 -0.8660375762491768 1212 90-91 0.03848623780643479 1212 92-93 -2.27679805148923 1212 94-95 -1.8979928394792331 1212 96-97 0.2566932091298746 1212 98-99 -0.5318523374258248 1212 100-101 0.06652151788170002 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 1.0 9 6.0 10 6.0 11 13.0 12 43.0 13 228.0 14 766.0 15 1550.0 16 2977.0 17 4869.0 18 7462.0 19 10591.0 20 14674.0 21 19866.0 22 25662.0 23 32923.0 24 41047.0 25 50597.0 26 61819.0 27 74692.0 28 88395.0 29 104888.0 30 122421.0 31 142765.0 32 164723.0 33 188521.0 34 211219.0 35 230856.0 36 243148.0 37 218441.0 38 75060.0 39 220.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.61296482970167 21.565114512654134 9.643948613546499 18.1779720440977 2 22.225534191278673 25.55745812085209 32.65286385393282 19.564143833936416 3 18.546674683698654 25.91253305974511 33.24428634633111 22.296505910225125 4 22.98451260249013 25.389053703660448 29.231002826508444 22.39543086734098 5 20.694620290125908 29.26725688523441 27.985283468429532 22.052839356210143 6 20.210236165292343 28.86262234576841 28.64734051250905 22.279800976430188 7 22.495970473498563 27.825550701955198 29.53187413861571 20.146604685930527 8 19.833539676236306 26.98297087061132 30.777827092433835 22.40566236071854 9 21.68679483286225 27.29374664206125 28.95222967133079 22.067228853745707 10-11 22.213272909902123 27.76002709710575 27.827933378495178 22.19876661449695 12-13 21.987883497145237 27.594990035539713 27.909733575023665 22.50739289229138 14-15 21.641796100209838 28.156739113321343 28.002075266001498 22.19938952046732 16-17 22.182451101221147 28.024235181331708 27.618524585109057 22.174789132338088 18-19 22.347142428836097 28.07203692071093 27.353183157802174 22.2276374926508 20-21 21.911350416968396 28.253451377046883 27.793127613352333 22.042070592632392 22-23 22.00425658255427 27.843894471698583 27.844011269595477 22.307837676151667 24-25 22.057464551846575 27.473498563386205 28.029876895045437 22.439159989721787 26-27 21.92489896984279 27.88060921768787 27.920016818893224 22.274474993576117 28-29 22.438389123782382 27.820551753136023 27.67095704174356 22.070102081338035 30-31 22.347146628045504 27.52232007288187 27.6082599453386 22.52227335373403 32-33 21.947370879955148 27.909925482959192 27.822607395641104 22.320096241444556 34-35 22.472657618725034 27.68338433506973 27.638323716975403 22.205634329229834 36-37 22.368100165852976 27.70646359410404 27.677264126702326 22.248172113340654 38-39 22.030717839706604 27.814665140507838 28.001167978696067 22.15344904108949 40-41 22.471389102828702 27.673618873486 27.602580733176215 22.252411290509087 42-43 22.153378962367725 27.551356023266138 27.866056203134853 22.429208811231284 44-45 22.033918101333832 27.6372258170011 28.085893153308884 22.242962928356185 46-47 22.430474946120185 27.696163645097922 27.74857146061493 22.124789948166963 48-49 22.383961316545587 27.50138989464832 27.79628115583172 22.318367632974375 50-51 22.06637485873291 27.791248471699166 27.869853474668165 22.27252319489976 52-53 22.35846586214605 27.647642676422883 27.811136601638957 22.1827548597921 54-55 22.122246615252866 27.419126513552772 27.96306410310917 22.495562768085193 56-57 22.029069535200925 27.756451480656015 27.976338283219714 22.23814070092335 58-59 22.375581449181563 27.567179680949415 27.8662574523933 22.19098141747572 60-61 22.339514991931484 27.407590479990358 27.765838261498455 22.487056266579707 62-63 22.106793109199646 27.684042236590123 27.976204658994337 22.23295999521589 64-65 22.3743041033375 27.660245448990008 27.70278414729182 22.262666300380676 66-67 22.36324454385647 27.606725496265234 27.720865040384513 22.309164919493778 68-69 22.1121183953959 27.847689645659003 27.891701067136808 22.148490891808294 70-71 22.54517592065904 27.64015310347566 27.54692210387629 22.267748871989003 72-73 22.268552219395893 27.609544296195203 27.768008309954766 22.353895174454134 74-75 22.000814222627724 27.72374295617968 28.031693919598556 22.243748901594042 76-77 22.415953685384803 27.766588864202568 27.67407755414509 22.14337989626754 78-79 22.437086680573433 27.68064584700845 27.51530639656707 22.366961075851048 80-81 22.14509558132247 27.700084659747333 27.869825114477752 22.284994644452446 82-83 22.38694502125457 27.70136941692381 27.617832204669906 22.293853357151715 84-85 22.263542067204927 27.47706098397972 27.75619770420618 22.503199244609174 86-87 22.13071957415604 27.761083709894034 27.85216845468271 22.25602826126721 88-89 22.407920185151312 27.652503488407138 27.597885066300176 22.341691260141378 90-91 22.43371806641079 27.471081934149964 27.545872754669055 22.549327244770193 92-93 22.077829236565893 27.71483265303071 27.800875048593042 22.406463061810353 94-95 22.449860231269646 27.59570916422621 27.459830361763295 22.494600242740844 96-97 22.3900046653679 27.567815456081178 27.466775314994102 22.57540456355682 98-99 22.177527311840215 27.731826488221365 27.629569164218676 22.461077035719743 100-101 22.4833955986217 27.508999867066663 27.344480264539673 22.663124269771963 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14.0 1 10.0 2 7.0 3 14.0 4 18.0 5 22.0 6 42.5 7 76.5 8 124.0 9 199.0 10 281.0 11 420.5 12 635.0 13 870.5 14 1207.5 15 1648.0 16 2228.0 17 3037.0 18 4086.0 19 5494.0 20 7416.5 21 9707.5 22 12290.0 23 15358.5 24 18957.0 25 22977.5 26 27302.0 27 31753.5 28 36380.5 29 41042.5 30 45571.5 31 49849.5 32 53590.0 33 57174.5 34 60283.5 35 62885.0 36 65486.0 37 66986.0 38 67681.5 39 67744.0 40 66815.0 41 65520.5 42 63226.5 43 60460.5 44 57772.0 45 55392.0 46 53335.0 47 51282.5 48 49391.5 49 46884.5 50 44287.0 51 41883.0 52 39462.0 53 37297.0 54 35221.5 55 33418.0 56 31933.5 57 30438.0 58 29128.0 59 28239.0 60 27258.5 61 26380.5 62 25856.0 63 25575.5 64 25396.0 65 25213.0 66 25078.5 67 24822.0 68 24229.5 69 23014.0 70 21652.0 71 20172.0 72 18093.5 73 15735.5 74 13176.5 75 10554.0 76 8172.5 77 6019.0 78 4125.5 79 2716.5 80 1748.5 81 1070.0 82 674.5 83 457.5 84 312.0 85 202.5 86 146.5 87 110.0 88 73.0 89 58.0 90 38.5 91 25.5 92 18.5 93 10.0 94 5.5 95 3.5 96 2.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7256418042934897 2 0.0030367446097783178 3 3.2703403489920345E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.19189889976406832 14-15 7.007872176411502E-5 16-17 3.737531827419468E-4 18-19 0.11506926113667687 20-21 0.0 22-23 2.3359573921371674E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0023593169660585393 42-43 0.0 44-45 0.0 46-47 0.023453012217057163 48-49 0.0 50-51 4.671914784274335E-5 52-53 1.6351701744960173E-4 54-55 0.0018220467658669906 56-57 0.0014249340092036722 58-59 0.09544721904272466 60-61 0.0014950127309677872 62-63 0.001448293583125044 64-65 0.002149080800766194 66-67 0.004087925436240043 68-69 0.004508397766824733 70-71 0.003153542479385176 72-73 0.03959447779672499 74-75 0.04606507977294494 76-77 0.1594991707351258 78-79 2.3359573921371673E-4 80-81 0.11586348665000351 82-83 0.004041206288397299 84-85 0.004718633932117079 86-87 0.006330444532691723 88-89 0.0068443551589619 90-91 0.022495269686280923 92-93 0.010558527412459997 94-95 0.014366137961643578 96-97 0.009881099768740218 98-99 0.012193697586956013 100-101 0.013058001822046765 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2140450.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.7671460282219 #Duplication Level Percentage of deduplicated Percentage of total 1 98.52325614105901 96.32337570318826 2 1.1261892216236884 2.2020861217178544 3 0.17522822316636869 0.5139468984771668 4 0.08991905781645894 0.35164518625047475 5 0.04890452537391021 0.23906279368359815 6 0.019340511092737125 0.11345199433584457 7 0.009226975751134315 0.06314665599620077 8 0.003235471977318667 0.025305828902138724 9 0.0028813421409461548 0.025353053806603704 >10 0.001509361480617507 0.025975209478984972 >50 0.0 0.0 >100 2.6131688050709E-4 0.031037334532585063 >500 0.0 0.0 >1k 4.7851637310063826E-5 0.08561321963026014 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 4.671914784274335E-5 0.0 0.0 6 4.671914784274335E-5 4.671914784274335E-5 0.0 7 9.34382956854867E-5 4.671914784274335E-5 0.0 8 1.868765913709734E-4 4.671914784274335E-5 0.0 9 2.8031488705646006E-4 4.671914784274335E-5 0.0 10-11 3.503936088205751E-4 4.671914784274335E-5 0.0 12-13 3.737531827419468E-4 4.671914784274335E-5 0.0 14-15 3.737531827419468E-4 4.671914784274335E-5 0.0 16-17 3.737531827419468E-4 4.671914784274335E-5 0.0 18-19 3.9711275666331846E-4 7.007872176411503E-5 0.0 20-21 4.204723305846901E-4 9.34382956854867E-5 0.0 22-23 4.438319045060618E-4 9.34382956854867E-5 0.0 24-25 5.139106262701768E-4 9.34382956854867E-5 0.0 26-27 7.007872176411502E-4 9.34382956854867E-5 0.0 28-29 9.577425307762386E-4 9.34382956854867E-5 0.0 30-31 0.0011913382699899554 9.34382956854867E-5 0.0 32-33 0.0014716531570464157 9.34382956854867E-5 0.0 34-35 0.0020322829311593357 9.34382956854867E-5 0.0 36-37 0.0029666658880142024 9.34382956854867E-5 0.0 38-39 0.003994487140554556 9.34382956854867E-5 0.0 40-41 0.005839893480342919 9.34382956854867E-5 0.0 42-43 0.008456165759536546 9.34382956854867E-5 0.0 44-45 0.011960101847742297 9.34382956854867E-5 9.34382956854867E-5 46-47 0.016421780466724288 9.34382956854867E-5 9.34382956854867E-5 48-49 0.022541988834123665 9.34382956854867E-5 1.1679786960685836E-4 50-51 0.030601041836996894 9.34382956854867E-5 1.4015744352823003E-4 52-53 0.04085589478847906 9.34382956854867E-5 1.4015744352823003E-4 54-55 0.05349342427994114 9.34382956854867E-5 1.4015744352823003E-4 56-57 0.06865378775491135 9.34382956854867E-5 1.4015744352823003E-4 58-59 0.08792543624004298 9.34382956854867E-5 1.4015744352823003E-4 60-61 0.11093461655259408 9.34382956854867E-5 1.4015744352823003E-4 62-63 0.13606951809199 9.34382956854867E-5 1.868765913709734E-4 64-65 0.1672311897030998 9.34382956854867E-5 1.868765913709734E-4 66-67 0.2041159569249457 9.34382956854867E-5 1.868765913709734E-4 68-69 0.2470742133663482 9.34382956854867E-5 1.868765913709734E-4 70-71 0.2970637015580836 9.34382956854867E-5 1.868765913709734E-4 72-73 0.3508608002990026 9.34382956854867E-5 1.868765913709734E-4 74-75 0.41164241164241167 9.34382956854867E-5 1.868765913709734E-4 76-77 0.481931369571819 9.34382956854867E-5 1.868765913709734E-4 78-79 0.5642271484968114 9.34382956854867E-5 1.868765913709734E-4 80-81 0.6540914293723282 9.34382956854867E-5 1.868765913709734E-4 82-83 0.7530893036511014 9.34382956854867E-5 1.868765913709734E-4 84-85 0.8618748394029292 9.34382956854867E-5 1.868765913709734E-4 86-87 0.9900254619355744 9.34382956854867E-5 1.868765913709734E-4 88-89 1.1321217500992782 9.34382956854867E-5 2.3359573921371673E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGTAT 680 0.0 17.801668 42-43 TATGCCG 825 0.0 16.686815 46-47 CGTATGC 815 0.0 16.309093 44-45 CTAATCG 945 0.0 15.816311 32-33 TCTCGTA 740 0.0 15.393339 40-41 TCGTATG 910 0.0 14.606497 42-43 GTATGCC 1030 0.0 13.365652 44-45 CCGTCTT 1150 0.0 12.790881 50-51 GCCGTCT 1065 0.0 12.6979 48-49 ATGCCGT 1110 0.0 11.760862 46-47 ACTAATC 1415 0.0 11.568822 32-33 GTCACTA 1360 0.0 11.164456 28-29 TAATCGA 1310 0.0 10.867438 34-35 AATCGAT 1415 0.0 10.564071 34-35 ATCGATC 1330 0.0 10.525619 36-37 AGTCACT 1650 0.0 10.064925 28-29 CAGTCAC 1635 0.0 10.012161 26-27 TCGATCT 1405 0.0 9.794875 36-37 ATCTCGT 1240 0.0 9.760494 40-41 CCAGTCA 1735 0.0 9.571832 26-27 >>END_MODULE