FastQCFastQC Report
Thu 11 Sep 2014
LONLib42_TAATCG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib42_TAATCG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140450
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT6800.017.80166842-43
TATGCCG8250.016.68681546-47
CGTATGC8150.016.30909344-45
CTAATCG9450.015.81631132-33
TCTCGTA7400.015.39333940-41
TCGTATG9100.014.60649742-43
GTATGCC10300.013.36565244-45
CCGTCTT11500.012.79088150-51
GCCGTCT10650.012.697948-49
ATGCCGT11100.011.76086246-47
ACTAATC14150.011.56882232-33
GTCACTA13600.011.16445628-29
TAATCGA13100.010.86743834-35
AATCGAT14150.010.56407134-35
ATCGATC13300.010.52561936-37
AGTCACT16500.010.06492528-29
CAGTCAC16350.010.01216126-27
TCGATCT14050.09.79487536-37
ATCTCGT12400.09.76049440-41
CCAGTCA17350.09.57183226-27
Produced by FastQC (version 0.11.2)