##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib41_GCGCTA_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5432302 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.887973275418044 27.0 22.0 33.0 2.0 33.0 2 24.913331769846373 33.0 15.0 33.0 2.0 33.0 3 25.796326308809782 33.0 22.0 33.0 6.0 33.0 4 27.935757989890842 33.0 22.0 37.0 2.0 37.0 5 27.99874601964324 33.0 15.0 37.0 6.0 37.0 6 29.055732910283705 33.0 27.0 37.0 15.0 37.0 7 28.747209746438987 33.0 27.0 37.0 6.0 37.0 8 28.519099637685827 33.0 27.0 37.0 6.0 37.0 9 29.26112631440594 33.0 27.0 37.0 15.0 37.0 10-11 28.889466104793144 33.0 24.5 37.0 10.5 37.0 12-13 29.18586291778329 33.0 27.0 37.0 10.5 37.0 14-15 29.536571144240508 33.0 27.0 37.0 6.0 40.0 16-17 30.38007100857058 33.0 27.0 37.0 15.0 40.0 18-19 30.702338529779823 33.0 27.0 37.0 15.0 40.0 20-21 30.723283241616535 33.0 27.0 37.0 15.0 40.0 22-23 30.74421184610134 33.0 27.0 37.0 15.0 40.0 24-25 30.35990644481842 33.0 27.0 37.0 10.5 40.0 26-27 29.861420719982064 33.0 27.0 37.0 15.0 40.0 28-29 30.046257645469638 33.0 27.0 37.0 15.0 40.0 30-31 29.974005863444262 33.0 27.0 37.0 15.0 40.0 32-33 29.821018050911015 33.0 27.0 37.0 15.0 40.0 34-35 29.855075067623268 33.0 27.0 37.0 15.0 40.0 36-37 30.091464999552677 33.0 27.0 37.0 15.0 40.0 38-39 30.130399414465543 33.0 27.0 37.0 15.0 40.0 40-41 30.21352384311476 33.0 27.0 37.0 15.0 40.0 42-43 30.12581645865786 33.0 27.0 37.0 15.0 40.0 44-45 29.886377819200774 33.0 27.0 37.0 15.0 40.0 46-47 29.52800700329253 33.0 27.0 37.0 15.0 40.0 48-49 29.39080255479169 33.0 24.5 37.0 10.5 40.0 50-51 28.646237359410428 33.0 24.5 37.0 10.5 38.5 52-53 28.38102217071142 33.0 22.0 37.0 10.5 37.0 54-55 29.329715376648792 33.0 24.5 37.0 15.0 40.0 56-57 29.251965373059157 33.0 24.5 37.0 15.0 40.0 58-59 29.453987002195387 33.0 27.0 37.0 15.0 40.0 60-61 29.216340420690898 33.0 24.5 37.0 10.5 40.0 62-63 29.08830768245212 33.0 24.5 37.0 15.0 40.0 64-65 29.05863333813179 33.0 27.0 37.0 15.0 40.0 66-67 28.94400624265735 33.0 24.5 37.0 15.0 40.0 68-69 28.66454598805442 33.0 22.0 37.0 15.0 40.0 70-71 28.57559115822353 33.0 22.0 37.0 15.0 38.5 72-73 28.338889940213193 33.0 22.0 37.0 15.0 37.0 74-75 28.287763824617997 33.0 22.0 37.0 15.0 37.0 76-77 27.898962815395755 33.0 22.0 37.0 15.0 37.0 78-79 27.34595729397961 33.0 22.0 37.0 6.0 37.0 80-81 27.21902639065354 30.0 22.0 33.0 6.0 37.0 82-83 26.80306359992504 27.0 22.0 33.0 6.0 37.0 84-85 26.89613905854277 27.0 22.0 33.0 6.0 37.0 86-87 26.592907205821767 27.0 22.0 33.0 6.0 37.0 88-89 26.235484422625987 27.0 22.0 33.0 6.0 37.0 90-91 26.108415824451587 27.0 22.0 33.0 6.0 37.0 92-93 25.998243838431662 27.0 22.0 33.0 6.0 37.0 94-95 25.795400274137926 27.0 22.0 33.0 6.0 37.0 96-97 25.66920856020155 27.0 22.0 33.0 6.0 37.0 98-99 25.4438161759048 27.0 22.0 33.0 6.0 37.0 100-101 24.98071995997277 27.0 18.5 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.29177727889332 2306 2 8.478035478945372 2306 3 7.481585828714437 2306 4 9.010918751901933 2306 5 8.828155523757594 2306 6 7.893870585692735 2306 7 8.079082745043145 2306 8 8.536403069295432 2306 9 7.391005685851642 2306 10-11 7.805277426033133 2306 12-13 7.406944671466867 2306 14-15 8.00489643089697 2306 16-17 6.789633381313859 2306 18-19 6.160371795887233 2306 20-21 6.089038106790262 2306 22-23 5.715324102434575 2306 24-25 5.847275927627027 2306 26-27 6.4911970004745605 2306 28-29 5.565995462835147 2306 30-31 5.489418309893566 2306 32-33 5.42509332661529 2306 34-35 4.839986233466462 2306 36-37 4.902374238836991 2306 38-39 4.5443894771635165 2306 40-41 3.750569009876461 2306 42-43 3.579385396183259 2306 44-45 3.385765992048267 2306 46-47 3.6073588735077102 2306 48-49 3.487193133898618 2306 50-51 3.2599453296064453 2306 52-53 3.8069708777251634 2306 54-55 3.337741637734709 2306 56-57 3.7062037857393975 2306 58-59 3.2352226893066174 2306 60-61 3.287713358912015 2306 62-63 2.7344266033392017 2306 64-65 2.388251000958185 2306 66-67 2.3731939018442176 2306 68-69 2.5172497507414846 2306 70-71 2.5607747498989113 2306 72-73 1.7828818468471361 2306 74-75 1.4611868045261502 2306 76-77 1.8564086822323418 2306 78-79 2.0411828597913804 2306 80-81 1.6662217943389805 2306 82-83 1.7526280027330472 2306 84-85 1.367146181690817 2306 86-87 1.3007363101903664 2306 88-89 1.367718802545948 2306 90-91 1.158132325482871 2306 92-93 1.1753080612699414 2306 94-95 1.430652434851087 2306 96-97 1.0737260955913293 2306 98-99 0.8920883228406176 2306 100-101 0.7251812626354344 2307 1 8.281643211167538 2307 2 8.371717772003581 2307 3 7.497153071522938 2307 4 9.178606772125121 2307 5 9.035529591209347 2307 6 7.659310008591799 2307 7 8.18757304791152 2307 8 8.475625284666975 2307 9 7.449756424011962 2307 10-11 7.728282248908908 2307 12-13 7.294859147629936 2307 14-15 8.099751381537857 2307 16-17 6.765520893216905 2307 18-19 6.389668724729265 2307 20-21 6.299608363896127 2307 22-23 5.925366562923305 2307 24-25 6.104227006951003 2307 26-27 6.851777218463059 2307 28-29 5.9668027122203 2307 30-31 5.825203506953251 2307 32-33 5.979195113956614 2307 34-35 5.69565180402499 2307 36-37 5.616344047908655 2307 38-39 5.214838360845885 2307 40-41 4.700407902533225 2307 42-43 4.460453759077257 2307 44-45 4.357494753240555 2307 46-47 4.774692371257267 2307 48-49 4.544704495744465 2307 50-51 4.280181438148553 2307 52-53 4.921645545981185 2307 54-55 4.472868094359658 2307 56-57 4.879369921478691 2307 58-59 4.444142029449523 2307 60-61 4.359621052906029 2307 62-63 4.084935656507298 2307 64-65 3.935477217124408 2307 66-67 3.5685251457214235 2307 68-69 3.8146085343354876 2307 70-71 3.4160758302504846 2307 72-73 2.9237292069392105 2307 74-75 2.536628378537422 2307 76-77 2.74377600861909 2307 78-79 3.13606786434708 2307 80-81 2.9425571006669458 2307 82-83 2.9784436715987823 2307 84-85 2.228082963654355 2307 86-87 2.2535138276424647 2307 88-89 2.4145233322127986 2307 90-91 2.2866662466041916 2307 92-93 2.1867889307577144 2307 94-95 2.351418196543481 2307 96-97 2.1729112556573718 2307 98-99 1.988351566909646 2307 100-101 1.6690176273928188 1101 1 8.02073839420364 1101 2 7.888996762224117 1101 3 7.309239477828402 1101 4 8.84380663298029 1101 5 8.6042704141582 1101 6 7.358323092621429 1101 7 7.145421537350188 1101 8 8.049349403384625 1101 9 7.329106634873224 1101 10-11 7.5443892090179325 1101 12-13 7.281311682468797 1101 14-15 7.733608449037568 1101 16-17 6.876858710951883 1101 18-19 6.066728056910161 1101 20-21 5.855065821579885 1101 22-23 5.266494506398633 1101 24-25 5.9652361191110295 1101 26-27 6.552397134849297 1101 28-29 5.969486686485098 1101 30-31 5.71651934289936 1101 32-33 5.719144221047138 1101 34-35 5.50731032853659 1101 36-37 5.424441426205767 1101 38-39 5.131959645971772 1101 40-41 4.663199059427146 1101 42-43 4.425783313374826 1101 44-45 4.357941854986034 1101 46-47 4.550287067007332 1101 48-49 4.365657230648427 1101 50-51 3.920537418293776 1101 52-53 4.819202506179014 1101 54-55 4.613113099899824 1101 56-57 4.765671157873442 1101 58-59 4.267886668478532 1101 60-61 4.4958174993336115 1101 62-63 4.121601710419071 1101 64-65 3.8564324068538767 1101 66-67 3.5318210959317256 1101 68-69 3.617747609144107 1101 70-71 3.524847816162449 1101 72-73 3.1084642773502047 1101 74-75 2.5460397481727455 1101 76-77 2.831560441701562 1101 78-79 3.333996162890397 1101 80-81 2.923110347291022 1101 82-83 3.123177931346472 1101 84-85 2.5596779697146665 1101 86-87 2.5507576421799527 1101 88-89 2.625581236408383 1101 90-91 2.452366809399898 1101 92-93 2.212992448605121 1101 94-95 2.35549983553976 1101 96-97 2.2864240043844752 1101 98-99 2.2441891038937847 1101 100-101 1.9614075160997828 2201 1 7.01361851628273 2201 2 7.777522220332447 2201 3 6.254124351100135 2201 4 8.33403028488722 2201 5 4.013006995652255 2201 6 5.691986803915267 2201 7 6.64172916174936 2201 8 7.722257644399178 2201 9 6.530625171570794 2201 10-11 4.093397076039825 2201 12-13 6.465578675336751 2201 14-15 7.070882655082535 2201 16-17 5.875091962599679 2201 18-19 2.0184270695172586 2201 20-21 5.050100596902581 2201 22-23 5.084716165520991 2201 24-25 5.390511881042382 2201 26-27 6.109169935623573 2201 28-29 4.959633640825487 2201 30-31 5.126889109304564 2201 32-33 5.281985444464219 2201 34-35 4.932175784778934 2201 36-37 4.9610614718697335 2201 38-39 4.369355366202495 2201 40-41 4.078330610402187 2201 42-43 3.98781444222028 2201 44-45 3.7621599657379186 2201 46-47 4.25550721907134 2201 48-49 4.031670127558339 2201 50-51 3.7507894024770962 2201 52-53 4.550090126140091 2201 54-55 4.124639669935068 2201 56-57 4.339279637904951 2201 58-59 3.9606771573192248 2201 60-61 3.8783129962624834 2201 62-63 3.713449713119786 2201 64-65 3.50562780355758 2201 66-67 3.201754207540734 2201 68-69 3.430550859378144 2201 70-71 3.2381712426641727 2201 72-73 2.6201908169468062 2201 74-75 2.3989706994794773 2201 76-77 2.753478863435415 2201 78-79 3.0094302403178297 2201 80-81 2.51290389186493 2201 82-83 2.859330715302871 2201 84-85 2.3348723691246427 2201 86-87 2.331661991375185 2201 88-89 2.442178199184749 2201 90-91 2.231800683702726 2201 92-93 2.1925620498063196 2201 94-95 2.3039242151195474 2201 96-97 2.156432897095474 2201 98-99 2.1778962646219817 2201 100-101 1.7531587491577731 2304 1 1.4426750242856734 2304 2 4.68842878371381 2304 3 5.723198652630558 2304 4 4.3455476190751625 2304 5 6.615427813729887 2304 6 6.404538612309754 2304 7 7.166592450278358 2304 8 6.539093935952003 2304 9 5.784799199066615 2304 10-11 6.685386130012322 2304 12-13 6.557563383552306 2304 14-15 6.874661109007413 2304 16-17 5.4848603029860605 2304 18-19 5.3732461133139005 2304 20-21 4.952889593085345 2304 22-23 4.588659932969318 2304 24-25 4.788010069210326 2304 26-27 5.751023784964001 2304 28-29 4.978401728702472 2304 30-31 4.986963976610717 2304 32-33 5.085495102864861 2304 34-35 4.766259948714236 2304 36-37 4.862156287492333 2304 38-39 4.517216831763147 2304 40-41 3.875504266737831 2304 42-43 3.457272425285602 2304 44-45 3.2392198443006492 2304 46-47 4.1709304089748045 2304 48-49 3.9187194614091077 2304 50-51 3.5677528550609736 2304 52-53 4.247545388454814 2304 54-55 3.8772623086831395 2304 56-57 4.535380608978514 2304 58-59 3.7635870966147174 2304 60-61 3.8207597249049385 2304 62-63 3.5552494594131048 2304 64-65 3.3617175559782737 2304 66-67 3.208861091111892 2304 68-69 3.450079510493488 2304 70-71 3.465167635992671 2304 72-73 3.0325925159248968 2304 74-75 2.4528144513072547 2304 76-77 2.632876128049606 2304 78-79 3.0500871436349932 2304 80-81 2.8467842793067746 2304 82-83 2.9875370612495153 2304 84-85 2.560438808562605 2304 86-87 2.399317920573502 2304 88-89 2.6149320861134164 2304 90-91 2.5703990479728276 2304 92-93 2.298882750159027 2304 94-95 2.5559978058235693 2304 96-97 2.545571364122136 2304 98-99 2.408519682464348 2304 100-101 2.0304147138509308 1102 1 7.011679909825212 1102 2 7.484738208229857 1102 3 6.017841545458964 1102 4 6.105664756327798 1102 5 6.595758179277631 1102 6 5.768774937795019 1102 7 6.7426480265774416 1102 8 6.31063764023224 1102 9 4.933748739699688 1102 10-11 6.004091293748896 1102 12-13 5.843087282728234 1102 14-15 6.121972900038848 1102 16-17 5.230830332383444 1102 18-19 5.252182958906662 1102 20-21 5.410227189652318 1102 22-23 5.158489580674068 1102 24-25 1.883431324725624 1102 26-27 5.3897054833858 1102 28-29 4.941907437263467 1102 30-31 4.862920154004705 1102 32-33 4.99419979329835 1102 34-35 4.940698306247384 1102 36-37 4.918682574219339 1102 38-39 3.8973272691477767 1102 40-41 4.031262887427484 1102 42-43 4.168505632229486 1102 44-45 -3.829035581593235 1102 46-47 1.4421388095785233 1102 48-49 -0.6936379455211004 1102 50-51 2.749482088888474 1102 52-53 -0.20847785598518698 1102 54-55 2.658134064584967 1102 56-57 -0.6141832317181866 1102 58-59 2.9130368029038607 1102 60-61 3.9046113180956894 1102 62-63 3.508935086250439 1102 64-65 3.077255180943432 1102 66-67 3.1069067122695913 1102 68-69 3.4383247445944463 1102 70-71 3.1870383348509606 1102 72-73 2.6864171308402547 1102 74-75 2.5010864848802186 1102 76-77 2.878491790174312 1102 78-79 -1.3423214431346082 1102 80-81 1.606665364538145 1102 82-83 2.699216151245462 1102 84-85 2.374176425113486 1102 86-87 2.394469282897525 1102 88-89 2.2635988123812076 1102 90-91 2.182353127056512 1102 92-93 2.215395595078647 1102 94-95 2.480379014639631 1102 96-97 2.4444657070985834 1102 98-99 2.374513697809885 1102 100-101 1.9624318158687757 2202 1 -20.541283945596476 2202 2 -7.582214906114311 2202 3 -0.8131703322405137 2202 4 1.3142773387328894 2202 5 0.9696047921441924 2202 6 1.5071382021732873 2202 7 3.8107432186665555 2202 8 2.5438966195457198 2202 9 3.0518173916660416 2202 10-11 1.9115020120939725 2202 12-13 1.3399519394684063 2202 14-15 2.5499066349786084 2202 16-17 1.5390681136973754 2202 18-19 1.5261850900728327 2202 20-21 1.099124552689375 2202 22-23 1.2934694624653815 2202 24-25 2.194602698901573 2202 26-27 3.305618929623577 2202 28-29 -1.1692105440959288 2202 30-31 0.8735637661892923 2202 32-33 1.3613010663149474 2202 34-35 1.447105216908895 2202 36-37 2.9164973616710306 2202 38-39 2.400262311202784 2202 40-41 1.6888053402576695 2202 42-43 0.49317197685326164 2202 44-45 0.5250920894683695 2202 46-47 -1.4041891626878993 2202 48-49 0.6360221192663502 2202 50-51 1.6126604817050207 2202 52-53 1.4835041853878508 2202 54-55 -1.043575073855589 2202 56-57 0.8732416659858373 2202 58-59 1.6993045626219825 2202 60-61 1.8824273558315383 2202 62-63 -2.201356656191024 2202 64-65 -0.1370005874130129 2202 66-67 0.3977550300690922 2202 68-69 1.243760099220502 2202 70-71 -2.7122109547603124 2202 72-73 -4.715563234015395 2202 74-75 -1.3439047915462439 2202 76-77 -0.3938824710214135 2202 78-79 1.3543135875541843 2202 80-81 1.478211397727506 2202 82-83 -0.9443163561425756 2202 84-85 -0.43092163694607066 2202 86-87 0.3736893329088886 2202 88-89 0.9309263015833018 2202 90-91 1.0344692045741297 2202 92-93 1.040047135201398 2202 94-95 1.47292447951801 2202 96-97 1.016174930785784 2202 98-99 1.0665938580735812 2202 100-101 0.7826744278260378 2305 1 5.63804427802841 2305 2 6.980016174920788 2305 3 6.583693555844963 2305 4 7.639892694229083 2305 5 7.746290081458042 2305 6 6.058379759244932 2305 7 2.9880611959365737 2305 8 5.951490682699674 2305 9 6.332089011517343 2305 10-11 4.8525957860191475 2305 12-13 5.188079661621629 2305 14-15 5.566858844176082 2305 16-17 5.033767570968077 2305 18-19 4.412614890419906 2305 20-21 5.057904772843514 2305 22-23 5.00229306693652 2305 24-25 5.203998540387186 2305 26-27 6.045306328606177 2305 28-29 5.123161046832493 2305 30-31 5.411623615279929 2305 32-33 5.33165617970964 2305 34-35 5.31762791286139 2305 36-37 4.661919784557533 2305 38-39 4.609514548472465 2305 40-41 4.093102833324689 2305 42-43 3.9608405589516984 2305 44-45 3.142131333135108 2305 46-47 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-19.65850023766488 1209 50-51 -21.894618677814705 1209 52-53 -24.546212795744225 1209 54-55 -21.78385458422515 1209 56-57 -25.419388050995295 1209 58-59 -25.70334873926481 1209 60-61 -25.439388681904312 1209 62-63 -25.59092033543631 1209 64-65 -25.488238794960182 1209 66-67 -13.286204310333218 1209 68-69 -25.182831881911575 1209 70-71 -25.044817086835785 1209 72-73 -15.454001270035977 1209 74-75 -16.623107680209895 1209 76-77 -13.572760823586517 1209 78-79 -14.265265782475442 1209 80-81 -24.107165960763062 1209 82-83 -15.960858925922711 1209 84-85 -13.075783148719033 1209 86-87 -13.390410932177652 1209 88-89 -10.61604810587031 1209 90-91 -13.452763272425713 1209 92-93 -17.487168578972934 1209 94-95 -22.826367973312177 1209 96-97 -18.621902509569743 1209 98-99 -10.037097374578844 1209 100-101 -5.384843699415502 1212 1 -20.541283945596476 1212 2 -20.321719623095444 1212 3 -21.226495803938626 1212 4 -23.214142472587866 1212 5 -23.408579170119957 1212 6 -24.46833349593992 1212 7 -24.045978479446653 1212 8 -23.67249488988824 1212 9 -24.597239212107542 1212 10-11 -24.314559308660744 1212 12-13 -24.615177777512727 1212 14-15 -24.937593365021392 1212 16-17 -25.97885641460451 1212 18-19 -20.637757485584654 1212 20-21 -26.403941485046474 1212 22-23 -26.60912487715726 1212 24-25 -22.703783644560712 1212 26-27 -25.34614993830095 1212 28-29 -20.234745616966578 1212 30-31 -15.315595176463777 1212 32-33 -25.5290291223643 1212 34-35 -21.692367761085407 1212 36-37 -25.940931883611988 1212 38-39 -26.24773297181608 1212 40-41 -20.801748053491554 1212 42-43 -17.13681207624116 1212 44-45 -14.136433936500833 1212 46-47 -17.366357064508634 1212 48-49 -20.200095170482214 1212 50-51 -12.358871711433164 1212 52-53 -5.2986745025275965 1212 54-55 -4.157464364140875 1212 56-57 -4.329398991914331 1212 58-59 -3.0490817808403037 1212 60-61 -14.370460891969957 1212 62-63 -12.041412676792984 1212 64-65 -16.343545140693223 1212 66-67 -12.544701154646653 1212 68-69 -8.198696214515515 1212 70-71 -15.47260702119027 1212 72-73 -12.03699311407815 1212 74-75 -8.359331616408966 1212 76-77 -19.18298787026174 1212 78-79 -9.951675533456509 1212 80-81 -7.988183466233522 1212 82-83 -5.349772881471839 1212 84-85 -4.712050486910979 1212 86-87 -3.6460229002771882 1212 88-89 -3.5326258587321284 1212 90-91 -3.764469603691424 1212 92-93 -3.6674333311294696 1212 94-95 -3.6182169886294666 1212 96-97 -13.359366567294394 1212 98-99 -10.558956155344527 1212 100-101 -6.674657794212685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 908.0 3 1999.0 4 863.0 5 5427.0 6 6961.0 7 6310.0 8 12755.0 9 23707.0 10 31206.0 11 38528.0 12 52026.0 13 66467.0 14 76838.0 15 83207.0 16 89098.0 17 98556.0 18 110895.0 19 123650.0 20 133707.0 21 148900.0 22 165283.0 23 178199.0 24 188053.0 25 195172.0 26 205159.0 27 214915.0 28 220581.0 29 224163.0 30 232652.0 31 245949.0 32 267661.0 33 309928.0 34 354461.0 35 389277.0 36 409155.0 37 380407.0 38 138864.0 39 415.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.25933155189854 22.361390470973994 14.944548114780378 20.434729862347087 2 25.140104912800716 28.77739247839182 25.922644119096798 20.159858489710665 3 19.32402521217539 27.322237402800564 29.872364631848402 23.48137275317565 4 22.439535360596143 27.184970781546923 28.00810180092599 22.367392056930946 5 21.447284957349225 28.606968841375945 27.567553703990548 22.37819249728428 6 20.96915926891532 29.175652526197965 27.31526673523569 22.53992146965103 7 22.317080755567424 28.287456925445298 28.715529685416012 20.679932633571262 8 21.018431543897428 27.905380704284422 29.104382209204005 21.97180554261414 9 21.50547928792075 27.52404464681212 28.871558562286737 22.0989175029804 10-11 22.418459186850573 27.883221038580718 27.53341589108651 22.1649038834822 12-13 22.049639397782258 28.082514112294177 27.686305968671444 22.181540521252117 14-15 22.04747629568045 28.30437258626734 27.68661749892349 21.961533619128723 16-17 22.377822086477643 28.285173042073254 27.397757696050025 21.939247175399082 18-19 22.3758886339376 28.42217087782135 27.290760104243233 21.91118038399781 20-21 22.260878572266 28.474831510041103 27.37882628621685 21.885463631476046 22-23 22.165298819082675 28.24122183271301 27.545627947038682 22.04785140116563 24-25 22.16054018646683 28.226544503669736 27.668273357845543 21.944641952017896 26-27 22.048946603103207 28.369760209727524 27.610252897282095 21.971040289887174 28-29 22.292934370053793 28.302936532228095 27.600304763616318 21.803824334101797 30-31 22.28475993251797 28.140784351574816 27.50364850345039 22.07080721245682 32-33 22.090246509276156 28.42807991534525 27.60274158977582 21.878931985602776 34-35 22.32708652304315 28.135141024825256 27.62726508109817 21.91050737103343 36-37 22.256783425089075 28.21305061032237 27.545235479426278 21.984930485162277 38-39 22.04177771169463 28.322314414223737 27.844510251899607 21.791397622182025 40-41 22.410855095908115 28.158732859720825 27.47192087485076 21.9584911695203 42-43 22.21504920401554 28.384589309181894 27.491338781022673 21.909022705779893 44-45 22.07668166917785 28.189294812391573 27.834833460197373 21.899190058233202 46-47 22.343890670173572 28.213068795927192 27.50389761584718 21.939142918052056 48-49 22.136885443370637 28.174809827502777 27.71598671237974 21.972318016746843 50-51 22.30832066533691 28.167099589193306 27.561737145220157 21.96284260024963 52-53 22.14666935069086 28.263397060050643 27.68669329004951 21.903240299208985 54-55 22.062385644110428 28.10840142405271 27.756255512960493 22.072957418876378 56-57 22.052365834747217 28.277515170554825 27.7505148331712 21.919604161526767 58-59 22.177143618152208 28.212802246713224 27.692815237276374 21.917238897858194 60-61 22.233139392885484 27.96429326422802 27.634554698539 22.168012644347503 62-63 22.141373142377034 28.22169308436199 27.749913471885723 21.887020301375244 64-65 22.25056370140215 28.09060989478412 27.696962025057058 21.961864378756673 66-67 22.346769211547404 28.07199428043896 27.57827955048693 22.002956957526706 68-69 22.180836038633494 28.186356567142234 27.753721903956162 21.879085490268107 70-71 22.446791434729867 28.04495903372894 27.537357040015088 21.970892491526104 72-73 22.297390475904464 28.201197731883564 27.611285899461013 21.89012589275096 74-75 22.22081043231454 28.09431305328356 27.7102734374964 21.9746030769055 76-77 22.500584996663832 28.169473094360075 27.44504798821704 21.884893920759048 78-79 22.413808946440295 28.109352414884704 27.591012924191187 21.885825714483815 80-81 22.472686530007085 28.08893355736906 27.509816640052964 21.928563272570887 82-83 22.441163120787756 28.14574448290391 27.533081121112545 21.88001127519579 84-85 22.462392043774713 27.899867463787402 27.51502430137574 22.122716191062143 86-87 22.44048964874331 28.080818466916867 27.549200463106537 21.929491421233283 88-89 22.536023065373723 28.032224019835382 27.524631409723487 21.907121505067405 90-91 22.635138247446957 27.915203884260762 27.34653820344833 22.103119664843955 92-93 22.56331137864612 28.08275828242488 27.41395764191926 21.939972697009736 94-95 22.77805852497334 27.924958955746167 27.308476944306843 21.988505574973647 96-97 22.797955461110362 27.982353603023135 27.197093186847216 22.02259774901929 98-99 22.72729156656201 28.05337048407688 27.301882119519185 21.91745582984193 100-101 23.161705693578686 27.850571557087626 26.939417789984184 22.048304959349505 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 360.0 1 373.5 2 423.0 3 464.0 4 494.5 5 563.5 6 657.5 7 787.5 8 997.0 9 1304.5 10 1723.5 11 2306.0 12 3076.0 13 4107.0 14 5413.0 15 6953.0 16 8731.0 17 11051.5 18 14194.5 19 18118.0 20 23021.5 21 29122.5 22 36277.5 23 44611.5 24 54245.0 25 64823.5 26 75858.5 27 87487.5 28 99459.5 29 110775.0 30 121646.0 31 132052.5 32 141960.5 33 151584.5 34 160283.0 35 168283.5 36 174909.0 37 179843.0 38 181827.5 39 180762.0 40 177464.5 41 171202.5 42 163033.5 43 154285.5 44 145314.5 45 137253.5 46 130832.0 47 124921.5 48 119339.5 49 113494.5 50 107407.0 51 101315.5 52 95777.5 53 91512.5 54 87784.0 55 84490.5 56 82176.0 57 80227.0 58 77819.5 59 75155.0 60 72583.5 61 69882.5 62 67042.5 63 64001.0 64 60935.0 65 57991.5 66 54731.0 67 50778.5 68 46657.0 69 42689.0 70 38029.0 71 33351.5 72 28866.0 73 24466.0 74 20276.0 75 16397.0 76 13242.5 77 10458.0 78 8169.0 79 6449.5 80 5034.5 81 3937.0 82 3163.0 83 2545.0 84 2021.0 85 1626.0 86 1299.0 87 1010.0 88 784.5 89 612.0 90 475.5 91 374.0 92 269.0 93 183.5 94 148.5 95 110.5 96 71.0 97 46.5 98 25.0 99 9.5 100 2.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.357302300203486 2 10.667853149548755 3 9.389702560719195 4 11.304857498717855 5 8.846617879491973 6 5.1243653243137075 7 9.431434408469928 8 9.714500408850613 9 5.2062458972273635 10-11 6.112215410704339 12-13 5.181486596290117 14-15 7.082660352830163 16-17 2.538178105709145 18-19 2.9703889805831856 20-21 2.9650045229444166 22-23 3.003063894459476 24-25 3.340176963651874 26-27 4.17300989525251 28-29 1.6651688363423096 30-31 2.1557895713456285 32-33 2.920428577056283 34-35 1.9259422616783823 36-37 2.9514927557414885 38-39 1.3263529899479078 40-41 1.122231422332558 42-43 0.5400749074701664 44-45 1.2329579614682689 46-47 1.3013083587768133 48-49 1.200246230787611 50-51 1.5314409986779085 52-53 2.3805653662112305 54-55 1.7048389430484534 56-57 1.7334271916399346 58-59 1.5143580014513185 60-61 1.3392664840798616 62-63 0.5950147837877938 64-65 1.1861177821115247 66-67 0.5483770968550717 68-69 0.7808752164367887 70-71 0.9700031404734126 72-73 0.08516647270346898 74-75 0.10637295201923604 76-77 0.4445905253426632 78-79 0.8355297625205668 80-81 0.2872907286818737 82-83 0.47390590582040537 84-85 0.06621502265522057 86-87 0.10074918515207733 88-89 0.08146638386452004 90-91 0.08946483461339226 92-93 0.167267946443331 94-95 0.44391861866295357 96-97 0.3615226104881503 98-99 0.06642671928033456 100-101 0.22450887303393663 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5432302.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.49626217640571 #Duplication Level Percentage of deduplicated Percentage of total 1 98.58601579721574 96.11768043092621 2 0.917307453142442 1.7886809589589305 3 0.2225413894548235 0.6509086095416725 4 0.11242334598928513 0.43843424061280445 5 0.07457549010383803 0.3635415767548871 6 0.03216827120604531 0.18817717219597896 7 0.023914017180787932 0.16320691021244194 8 0.011275471479985345 0.08794530588601893 9 0.008597154930969887 0.07543714240089172 >10 0.011181609296076568 0.12598765251014912 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.52252065514767E-5 0.0 0.0 2 5.52252065514767E-5 1.8408402183825567E-5 1.8408402183825567E-5 3 7.363360873530227E-5 1.8408402183825567E-5 1.8408402183825567E-5 4 1.104504131029534E-4 1.8408402183825567E-5 1.8408402183825567E-5 5 1.104504131029534E-4 1.8408402183825567E-5 5.52252065514767E-5 6 1.104504131029534E-4 1.8408402183825567E-5 5.52252065514767E-5 7 1.104504131029534E-4 1.8408402183825567E-5 5.52252065514767E-5 8 1.2885881528677898E-4 1.8408402183825567E-5 5.52252065514767E-5 9 1.2885881528677898E-4 3.6816804367651134E-5 7.363360873530227E-5 10-11 1.4726721747060454E-4 3.6816804367651134E-5 1.0124621201104063E-4 12-13 1.6567561965443012E-4 3.6816804367651134E-5 1.2885881528677898E-4 14-15 2.1169662511399403E-4 3.6816804367651134E-5 1.2885881528677898E-4 16-17 2.6692183166547074E-4 3.6816804367651134E-5 1.2885881528677898E-4 18-19 2.945344349412091E-4 3.6816804367651134E-5 1.4726721747060454E-4 20-21 3.1294283712503463E-4 3.6816804367651134E-5 1.6567561965443012E-4 22-23 3.773722447684241E-4 3.6816804367651134E-5 1.6567561965443012E-4 24-25 3.8657644586033695E-4 3.6816804367651134E-5 1.748798207463429E-4 26-27 4.418016524118136E-4 5.52252065514767E-5 1.8408402183825567E-4 28-29 5.154352611471159E-4 5.52252065514767E-5 1.8408402183825567E-4 30-31 6.995192829853716E-4 5.52252065514767E-5 1.9328822293016848E-4 32-33 0.0010124621201104062 5.52252065514767E-5 2.0249242402208125E-4 34-35 0.0014726721747060456 6.442940764338949E-5 2.0249242402208125E-4 36-37 0.002052536843496551 7.363360873530227E-5 2.0249242402208125E-4 38-39 0.00307420316469887 7.363360873530227E-5 2.0249242402208125E-4 40-41 0.004648121551415956 7.363360873530227E-5 2.1169662511399403E-4 42-43 0.006571799579625727 9.204201091912784E-5 2.6692183166547074E-4 44-45 0.009047729673350267 9.204201091912784E-5 3.1294283712503463E-4 46-47 0.012876677327585986 1.104504131029534E-4 3.3135123930886024E-4 48-49 0.01843601478710131 1.104504131029534E-4 3.497596414926858E-4 50-51 0.025900621872642575 1.104504131029534E-4 3.6816804367651134E-4 52-53 0.034432916284845724 1.104504131029534E-4 3.6816804367651134E-4 54-55 0.04590135084536905 1.104504131029534E-4 3.773722447684241E-4 56-57 0.06024149614656917 1.104504131029534E-4 4.049848480441625E-4 58-59 0.07800560425396084 1.104504131029534E-4 4.418016524118136E-4 60-61 0.09887152812932712 1.104504131029534E-4 4.5100585350372644E-4 62-63 0.12355719545783721 1.4726721747060454E-4 4.602100545956392E-4 64-65 0.15208101464167495 1.4726721747060454E-4 4.602100545956392E-4 66-67 0.1854554478009507 1.4726721747060454E-4 4.786184567794648E-4 68-69 0.22474818226232635 1.4726721747060454E-4 4.8782265787137755E-4 70-71 0.26879028448712905 1.4726721747060454E-4 5.246394622390287E-4 72-73 0.31580534366461954 1.6567561965443012E-4 5.338436633309415E-4 74-75 0.36861904953001506 1.6567561965443012E-4 5.52252065514767E-4 76-77 0.4286672574536541 1.6567561965443012E-4 5.614562666066798E-4 78-79 0.497477864816794 1.9328822293016848E-4 5.706604676985926E-4 80-81 0.5750324632172512 2.0249242402208125E-4 5.706604676985926E-4 82-83 0.6577045974248118 2.0249242402208125E-4 5.982730709743309E-4 84-85 0.7499583049690537 2.0249242402208125E-4 6.074772720662437E-4 86-87 0.8548954016179513 2.0249242402208125E-4 6.074772720662437E-4 88-89 0.9713837706371995 2.0249242402208125E-4 6.258856742500693E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGTAG 3000 0.0 5.681173 94-95 TCGGAAG 8225 0.0 5.138957 94-95 >>END_MODULE