##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib41_GCGCTA_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5432302 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.223807696994754 33.0 33.0 33.0 27.0 33.0 2 31.60017834060036 33.0 33.0 33.0 27.0 33.0 3 31.77808229365746 33.0 33.0 33.0 27.0 33.0 4 35.61083478054055 37.0 37.0 37.0 33.0 37.0 5 35.14178501121624 37.0 37.0 37.0 33.0 37.0 6 34.95412184374138 37.0 33.0 37.0 33.0 37.0 7 34.76189652195331 37.0 33.0 37.0 27.0 37.0 8 34.80370734174941 37.0 33.0 37.0 27.0 37.0 9 34.712219423736016 37.0 33.0 37.0 27.0 37.0 10-11 34.568546814959845 37.0 33.0 37.0 27.0 37.0 12-13 34.576097021115544 37.0 33.0 37.0 27.0 37.0 14-15 35.51210674590625 37.0 33.0 40.0 27.0 40.0 16-17 35.421374400760484 37.0 33.0 40.0 27.0 40.0 18-19 35.63915537096428 37.0 33.0 40.0 27.0 40.0 20-21 35.33042391604885 37.0 33.0 40.0 27.0 40.0 22-23 35.35823947195867 37.0 33.0 40.0 27.0 40.0 24-25 35.207507885239075 37.0 33.0 40.0 27.0 40.0 26-27 34.83850658523771 37.0 33.0 40.0 27.0 40.0 28-29 34.49097758187965 37.0 33.0 40.0 27.0 40.0 30-31 34.395921379186944 37.0 33.0 40.0 27.0 40.0 32-33 34.39527036604372 37.0 33.0 40.0 27.0 40.0 34-35 34.25836082014586 37.0 33.0 40.0 24.5 40.0 36-37 33.96948641662411 37.0 33.0 37.0 22.0 40.0 38-39 33.88408229144845 37.0 33.0 37.0 22.0 40.0 40-41 33.62632471464215 37.0 33.0 37.0 22.0 40.0 42-43 33.26496483442931 37.0 33.0 37.0 22.0 40.0 44-45 33.15620652165509 37.0 33.0 37.0 22.0 40.0 46-47 33.03073503645416 37.0 33.0 37.0 22.0 40.0 48-49 33.05822430343527 37.0 33.0 37.0 22.0 40.0 50-51 33.33953717963398 37.0 33.0 37.0 22.0 40.0 52-53 33.205734603856705 37.0 33.0 37.0 22.0 40.0 54-55 32.85952465455713 37.0 33.0 37.0 22.0 40.0 56-57 32.91271867801164 37.0 33.0 37.0 22.0 40.0 58-59 32.6357534061987 37.0 33.0 37.0 22.0 40.0 60-61 32.54458800339157 37.0 33.0 37.0 22.0 40.0 62-63 32.52412218613766 37.0 33.0 37.0 22.0 40.0 64-65 32.194344128879436 35.0 27.0 37.0 22.0 40.0 66-67 31.854090770358496 33.0 27.0 37.0 18.5 40.0 68-69 31.68309098058245 33.0 27.0 37.0 22.0 40.0 70-71 31.529924606548015 33.0 27.0 37.0 18.5 40.0 72-73 31.30463245600116 33.0 27.0 37.0 15.0 38.5 74-75 30.960415216237976 33.0 27.0 37.0 15.0 37.0 76-77 29.914551418533065 33.0 27.0 37.0 15.0 37.0 78-79 30.812092553028165 33.0 27.0 37.0 15.0 37.0 80-81 30.515149562745222 33.0 27.0 37.0 15.0 37.0 82-83 30.489015430290877 33.0 27.0 37.0 15.0 37.0 84-85 30.357246154576828 33.0 27.0 37.0 15.0 37.0 86-87 30.14564046696962 33.0 27.0 37.0 15.0 37.0 88-89 30.008317560400727 33.0 27.0 37.0 15.0 37.0 90-91 29.911004579642295 33.0 27.0 37.0 15.0 37.0 92-93 29.69368197865288 33.0 27.0 37.0 15.0 37.0 94-95 29.605582863397508 33.0 27.0 33.0 15.0 37.0 96-97 29.467529051219906 33.0 27.0 33.0 15.0 37.0 98-99 29.20623245909377 33.0 27.0 33.0 15.0 37.0 100-101 28.432865385613688 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.1148705525509612 2306 2 0.6250770007479574 2306 3 0.4822784832655067 2306 4 0.3700145670915944 2306 5 0.6209639879992821 2306 6 0.7262008199177856 2306 7 0.7943555666489104 2306 8 0.8287353844166176 2306 9 0.9879634731000024 2306 10-11 1.1120957242640586 2306 12-13 1.3346321842677398 2306 14-15 1.4168521237087006 2306 16-17 1.4525747710062973 2306 18-19 1.353301427022764 2306 20-21 1.5537154871944878 2306 22-23 1.2138478545181215 2306 24-25 1.1735024352592731 2306 26-27 1.2588829247243893 2306 28-29 1.6244662401007162 2306 30-31 1.584533020869479 2306 32-33 1.2290704561884098 2306 34-35 1.1869385118842999 2306 36-37 1.46311738659778 2306 38-39 1.1883373754465794 2306 40-41 1.245013521982301 2306 42-43 1.2324856444206134 2306 44-45 1.0241735747574339 2306 46-47 1.0414212691193754 2306 48-49 0.9838601338712465 2306 50-51 1.1568881122140695 2306 52-53 1.2348700792260203 2306 54-55 1.6625317623865215 2306 56-57 1.268696814063233 2306 58-59 1.1581277536452674 2306 60-61 0.9845838885216267 2306 62-63 0.7374829194873129 2306 64-65 0.8498134665943375 2306 66-67 1.0088936459712805 2306 68-69 0.8928167838837382 2306 70-71 0.8204510295038467 2306 72-73 0.6006682977737476 2306 74-75 0.8509192611821419 2306 76-77 0.8365831764940737 2306 78-79 0.8412714251065196 2306 80-81 1.326939244884123 2306 82-83 1.2322092128272146 2306 84-85 0.9776329827393937 2306 86-87 0.8789105615982535 2306 88-89 0.695347443720042 2306 90-91 0.3987171379750194 2306 92-93 0.11254649836372366 2306 94-95 0.07961178044700645 2306 96-97 0.16579501947995468 2306 98-99 0.3360516980049759 2306 100-101 0.4031520168944134 2307 1 0.6871635970598042 2307 2 0.4297881145993365 2307 3 0.35842654007131003 2307 4 0.34644663921551455 2307 5 0.48448509835682074 2307 6 0.5437552930665674 2307 7 0.7326898248234812 2307 8 0.7377280473428129 2307 9 0.9461980539414014 2307 10-11 1.1131413155116334 2307 12-13 1.2851411826304258 2307 14-15 1.2597169256214045 2307 16-17 1.3445462173894924 2307 18-19 1.1415535250422337 2307 20-21 1.3833500995489914 2307 22-23 1.1792817146684271 2307 24-25 1.237379712252654 2307 26-27 1.3262688918000407 2307 28-29 1.5589873436075834 2307 30-31 1.6211379629470102 2307 32-33 1.3322543899834756 2307 34-35 1.2102961103113827 2307 36-37 1.371693877818636 2307 38-39 0.9923408692912403 2307 40-41 1.1974936515970924 2307 42-43 1.2054567482700023 2307 44-45 1.0858692334841606 2307 46-47 0.9960403509917981 2307 48-49 0.9897278336198241 2307 50-51 0.9949215914667988 2307 52-53 1.2053486331853733 2307 54-55 1.5030429872188549 2307 56-57 1.1846637527017236 2307 58-59 1.144623852185397 2307 60-61 0.9294864034047237 2307 62-63 0.8211229027716627 2307 64-65 0.9478461580193489 2307 66-67 0.9814048708036047 2307 68-69 0.8391569062368092 2307 70-71 0.822613417923467 2307 72-73 0.7277678449440756 2307 74-75 0.8932930614575412 2307 76-77 0.9536815590495102 2307 78-79 0.9703759732701975 2307 80-81 1.5930103754511542 2307 82-83 1.229134595266192 2307 84-85 1.079155833168592 2307 86-87 1.0512716675713563 2307 88-89 0.9254729001463211 2307 90-91 0.7452265953329871 2307 92-93 0.7716771476022686 2307 94-95 0.6833064732284484 2307 96-97 0.6205247123641584 2307 98-99 0.7263289380046984 2307 100-101 0.7374737794362858 1101 1 1.0375123598911813 1101 2 0.5830775046532217 1101 3 0.3664840766139541 1101 4 0.335007596402086 1101 5 0.5489206521464283 1101 6 0.6153204974184163 1101 7 0.7614152794229057 1101 8 0.7286980954269495 1101 9 0.8631058263375948 1101 10-11 0.9381679506854894 1101 12-13 1.19666359436264 1101 14-15 1.2804706674224775 1101 16-17 1.2837025109911906 1101 18-19 1.049238606832951 1101 20-21 1.3103878647874936 1101 22-23 0.9910614263666133 1101 24-25 1.0882928946529091 1101 26-27 1.1785456441331377 1101 28-29 1.3870563131669797 1101 30-31 1.368476919416544 1101 32-33 0.9775758731700179 1101 34-35 1.116357677081247 1101 36-37 1.2408446058805467 1101 38-39 0.9721744460773039 1101 40-41 1.0933408924366574 1101 42-43 1.178041199976164 1101 44-45 1.0354807050169441 1101 46-47 0.8818079324266748 1101 48-49 0.8302604026207234 1101 50-51 0.9067470187396438 1101 52-53 1.1200804596744902 1101 54-55 1.5161546732792743 1101 56-57 1.1969003917906207 1101 58-59 1.219396251167737 1101 60-61 0.8982338423303133 1101 62-63 0.7728196121890818 1101 64-65 0.761005202547981 1101 66-67 0.9042125340203278 1101 68-69 0.755459599034495 1101 70-71 0.9958841360798125 1101 72-73 0.8205697003100667 1101 74-75 0.9253739517003226 1101 76-77 0.9130639021176563 1101 78-79 0.9252631946538443 1101 80-81 1.5849259753787877 1101 82-83 1.2404976146076798 1101 84-85 1.1368850193565123 1101 86-87 0.8920218406777849 1101 88-89 0.9153025961513883 1101 90-91 0.7295472379162327 1101 92-93 0.8103771861944118 1101 94-95 0.7726331964208057 1101 96-97 0.7654100358568776 1101 98-99 0.8228181379142718 1101 100-101 0.7842802607845627 2201 1 1.420291359872099 2201 2 0.7611845786628457 2201 3 0.2961419664047362 2201 4 0.2225270468598879 2201 5 0.37828111162870215 2201 6 0.49634882473689146 2201 7 0.6114417141669506 2201 8 0.6582874757361523 2201 9 0.6716610379935801 2201 10-11 0.845371106688944 2201 12-13 1.0816270852692895 2201 14-15 1.0930296272682654 2201 16-17 1.0011184775765827 2201 18-19 0.8765272554578942 2201 20-21 1.1399046490846487 2201 22-23 0.8846314208963761 2201 24-25 0.8699597874309148 2201 26-27 1.028912447803748 2201 28-29 1.3678641931996651 2201 30-31 1.418759348251946 2201 32-33 1.0575214802474093 2201 34-35 0.9241851257355691 2201 36-37 1.1266896305221792 2201 38-39 0.7410794196703563 2201 40-41 1.0932352736525601 2201 42-43 1.0910154534819796 2201 44-45 0.8903075360342569 2201 46-47 0.9025554129379003 2201 48-49 1.0187211222737602 2201 50-51 0.9257991900620297 2201 52-53 1.1598734258240526 2201 54-55 1.4075530224111077 2201 56-57 1.047908720650554 2201 58-59 1.1296760237583285 2201 60-61 0.895714916961623 2201 62-63 0.8160489448240966 2201 64-65 0.7979270805551302 2201 66-67 0.7058953131855681 2201 68-69 0.8773218159502321 2201 70-71 0.9193971310213733 2201 72-73 0.803541187895803 2201 74-75 0.9219650791515477 2201 76-77 0.9129219252314904 2201 78-79 0.894920555599505 2201 80-81 1.3462007054612002 2201 82-83 1.2544538673974515 2201 84-85 1.1539919570983699 2201 86-87 1.1378754770814403 2201 88-89 1.0736606852957458 2201 90-91 0.8847060394390063 2201 92-93 0.9916640917338064 2201 94-95 0.9520313586539615 2201 96-97 0.7186399198730271 2201 98-99 0.9096755403862922 2201 100-101 0.9271168958692577 2304 1 -1.9231753769513489 2304 2 -0.33353038039743055 2304 3 -0.10861995694864035 2304 4 0.024822324153390696 2304 5 0.4188702583205952 2304 6 0.5547911868371429 2304 7 0.505793936846402 2304 8 0.6471799812863566 2304 9 0.9258111289672399 2304 10-11 0.8659128257882145 2304 12-13 1.1372281543013187 2304 14-15 1.2342299909621062 2304 16-17 1.2408376808843542 2304 18-19 0.9080535003531836 2304 20-21 1.2142805732900328 2304 22-23 0.9675903963187835 2304 24-25 1.1280766342099184 2304 26-27 1.1767243717353182 2304 28-29 1.449185594450924 2304 30-31 1.5268408981888868 2304 32-33 1.0371098828713485 2304 34-35 1.026399071839407 2304 36-37 1.2388816994813752 2304 38-39 1.0456697585962615 2304 40-41 1.0807775409028366 2304 42-43 1.0128196042812903 2304 44-45 1.1952098212944549 2304 46-47 1.1047240599869212 2304 48-49 0.9815801919512239 2304 50-51 0.9837602560956356 2304 52-53 1.1654950179837158 2304 54-55 1.5536645980611752 2304 56-57 1.1867852524067075 2304 58-59 0.9987845205860069 2304 60-61 0.71726198114672 2304 62-63 0.5018686079399046 2304 64-65 0.5138546125158179 2304 66-67 0.7527816104106542 2304 68-69 0.6657947277457055 2304 70-71 0.589539334227748 2304 72-73 0.49865435679796377 2304 74-75 0.7037975070243476 2304 76-77 0.8434133371048027 2304 78-79 0.754417186248169 2304 80-81 1.3892342590923406 2304 82-83 1.058098718345704 2304 84-85 0.8196217586949999 2304 86-87 0.7343064942183553 2304 88-89 0.5176006439088958 2304 90-91 0.40211845101005395 2304 92-93 0.5105265266910735 2304 94-95 0.27611871530301 2304 96-97 0.24279122468399095 2304 98-99 0.6189010552948133 2304 100-101 0.6732584493408069 1102 1 1.5209605451745212 1102 2 0.1566747386382943 1102 3 0.21393621623821346 1102 4 0.14102317294357647 1102 5 0.2559529233324511 1102 6 0.11111844962027106 1102 7 -4.8147331965672215 1102 8 -1.1232921543097163 1102 9 0.2641709970734496 1102 10-11 0.6573875855869034 1102 12-13 0.8521979575650107 1102 14-15 0.8108806459609781 1102 16-17 0.5717845079131152 1102 18-19 0.7989881740107094 1102 20-21 1.112381334256206 1102 22-23 0.7161635663450241 1102 24-25 1.0314375225472006 1102 26-27 0.9900479112009108 1102 28-29 0.9313283005875661 1102 30-31 1.196234028983561 1102 32-33 1.000328385866311 1102 34-35 1.2087870488894694 1102 36-37 1.2350155916629362 1102 38-39 0.8780555952646267 1102 40-41 1.143085319375956 1102 42-43 1.1693998317823002 1102 44-45 1.0408910569310237 1102 46-47 0.8761485865523682 1102 48-49 0.9013639450342623 1102 50-51 0.7206122331877651 1102 52-53 0.8503871679892328 1102 54-55 1.2623439089318893 1102 56-57 1.020398805703259 1102 58-59 0.9313424081817843 1102 60-61 0.8117090421975632 1102 62-63 0.6256427424526407 1102 64-65 0.41588673927690323 1102 66-67 0.5463684431190536 1102 68-69 0.6132967445427013 1102 70-71 0.47355503737205495 1102 72-73 0.4748650029618453 1102 74-75 0.5289954844425324 1102 76-77 0.5091870614215388 1102 78-79 0.5362313464244473 1102 80-81 1.0304494489657543 1102 82-83 0.8686257360630165 1102 84-85 0.594125237472614 1102 86-87 0.49499612835507634 1102 88-89 0.4640354201154189 1102 90-91 -0.007915269204719522 1102 92-93 0.3271405480317817 1102 94-95 0.17106641531300326 1102 96-97 0.05858577433611245 1102 98-99 0.1374896129299792 1102 100-101 0.3161134135012489 2202 1 1.5163688197823824 2202 2 0.899177693855389 2202 3 0.38843934194860097 2202 4 -0.254015756358541 2202 5 0.039614835130592496 2202 6 0.4438468538030378 2202 7 0.6676319432372821 2202 8 -3.9989789012976686 2202 9 -0.5551747633266402 2202 10-11 0.3852994689694853 2202 12-13 -0.012344182694135952 2202 14-15 0.10496018108258909 2202 16-17 0.3068337236439618 2202 18-19 0.7625419930604949 2202 20-21 1.2518389950882067 2202 22-23 -2.633454272936632 2202 24-25 -2.7921704565389476 2202 26-27 -1.521363176546302 2202 28-29 0.49346676955778435 2202 30-31 1.1794868137073635 2202 32-33 1.074926106939273 2202 34-35 -2.0619476067140816 2202 36-37 -0.3189056688508174 2202 38-39 0.3108508044030671 2202 40-41 1.2033602682279678 2202 42-43 1.2258537133462895 2202 44-45 1.266801462705061 2202 46-47 0.6184939491347805 2202 48-49 0.9027176617880741 2202 50-51 -4.052752338305758 2202 52-53 -0.2970236924335907 2202 54-55 1.4511067527600332 2202 56-57 1.2712519257305388 2202 58-59 1.386954621184735 2202 60-61 1.3684289501311753 2202 62-63 1.1564012387017897 2202 64-65 1.1693133703294158 2202 66-67 1.2810016552127088 2202 68-69 1.204008014149423 2202 70-71 1.1397509909978858 2202 72-73 0.9959014032801008 2202 74-75 0.5166405742356659 2202 76-77 1.1883849188891453 2202 78-79 1.1838732252605446 2202 80-81 1.7742914300978327 2202 82-83 1.6754656144444624 2202 84-85 1.4642522549765928 2202 86-87 1.3827064318331388 2202 88-89 1.3275159554643636 2202 90-91 1.0637413004702054 2202 92-93 1.1089671279363031 2202 94-95 1.0803750650061126 2202 96-97 0.9699133772004025 2202 98-99 1.1715170907658496 2202 100-101 1.2583715971793552 2305 1 1.3429680848010115 2305 2 0.6732111167929311 2305 3 0.15408417326016988 2305 4 -0.17377997110239818 2305 5 0.19662214666401212 2305 6 0.38489405338791016 2305 7 0.4704222566251701 2305 8 -0.08540811680713745 2305 9 0.4614877917544291 2305 10-11 0.3480312241628454 2305 12-13 0.8030934566035484 2305 14-15 0.6165131163293722 2305 16-17 0.7254945093254364 2305 18-19 0.7383221664276292 2305 20-21 0.8179823207087722 2305 22-23 0.5567547480239483 2305 24-25 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-0.7403477336884592 1208 42-43 0.11674790710489447 1208 44-45 0.11008893819138166 1208 46-47 -0.17756866074540767 1208 48-49 -1.531740661902198 1208 50-51 -0.3480743608319514 1208 52-53 -1.6587334306091606 1208 54-55 -0.8444349793779651 1208 56-57 -6.404632127584442 1208 58-59 -2.036199962722101 1208 60-61 -1.3876748432473853 1208 62-63 0.32421724446326294 1208 64-65 -0.09014015720508439 1208 66-67 0.7845079242196995 1208 68-69 0.4263001020657562 1208 70-71 -4.067189985326873 1208 72-73 -0.6447399138001018 1208 74-75 -5.712482064228588 1208 76-77 -1.2387903407630567 1208 78-79 -4.025785179329958 1208 80-81 0.7624899051740641 1208 82-83 0.39832037517465935 1208 84-85 -2.778158526375549 1208 86-87 -1.0091450898424412 1208 88-89 -4.978981071934118 1208 90-91 -0.12449763741221886 1208 92-93 -2.126343503085536 1208 94-95 -4.65479631255133 1208 96-97 -3.6031427459425487 1208 98-99 -6.276562048849481 1208 100-101 -3.7560398865939035 1209 1 1.7822701140284458 1209 2 1.315069242272667 1209 3 0.6831541789983469 1209 4 0.45715978550953906 1209 5 0.5619590967866017 1209 6 1.0078935168312668 1209 7 0.7939271638828416 1209 8 0.11796324746266151 1209 9 -0.070994192610911 1209 10-11 -2.579787561281563 1209 12-13 -0.14500586599368148 1209 14-15 -1.094636828897137 1209 16-17 -0.07497861067695055 1209 18-19 -16.84073163082848 1209 20-21 -14.004463632387232 1209 22-23 -3.323366852852427 1209 24-25 -2.123744992637505 1209 26-27 -1.5907412952115152 1209 28-29 -3.094395287981591 1209 30-31 -0.6071536041937762 1209 32-33 -0.44106143789153407 1209 34-35 -3.140010720701291 1209 36-37 -3.573810257187013 1209 38-39 -1.539372862367557 1209 40-41 -10.4992691563783 1209 42-43 -5.196775613801687 1209 44-45 -2.7862416723331407 1209 46-47 -2.498299088445144 1209 48-49 -2.6643375385861674 1209 50-51 -2.3403390761819445 1209 52-53 -1.0838936246026165 1209 54-55 -3.0017984788008576 1209 56-57 -0.7839781732870819 1209 58-59 -1.879260001456771 1209 60-61 -1.4998215761435745 1209 62-63 -0.7660019852183737 1209 64-65 -0.36175340808942025 1209 66-67 0.06292704114753533 1209 68-69 -0.8900494667487457 1209 70-71 -0.8340600178903124 1209 72-73 -0.2829711017877301 1209 74-75 0.8267674649264478 1209 76-77 -16.643180358672062 1209 78-79 -16.5770065307039 1209 80-81 -22.043467906406146 1209 82-83 -9.714791967810328 1209 84-85 -3.370662176646672 1209 86-87 -2.168415800351486 1209 88-89 -1.5790863562462256 1209 90-91 -5.959055167641441 1209 92-93 -3.468143331287145 1209 94-95 -0.6422752156737559 1209 96-97 0.23981289770562242 1209 98-99 1.0849833896899135 1209 100-101 0.4596063936864496 1212 1 -28.60202740663271 1212 2 -16.215011955622337 1212 3 -5.740060461940583 1212 4 -1.4059141606358168 1212 5 -0.4132176759135433 1212 6 -2.485295987120125 1212 7 -0.3784899654370122 1212 8 -1.3497447656302697 1212 9 -0.0031641114130138703 1212 10-11 0.7430075270595822 1212 12-13 0.15286743635545008 1212 14-15 -0.18949052801282562 1212 16-17 -0.1359199818182404 1212 18-19 -0.8389874132398276 1212 20-21 -0.6643213100189271 1212 22-23 -0.5573130886337481 1212 24-25 0.14785111565047515 1212 26-27 -0.4641141725828035 1212 28-29 0.11804526033025553 1212 30-31 -0.7041864450350488 1212 32-33 0.15477920445795945 1212 34-35 -1.2708270398506158 1212 36-37 -1.585835321837898 1212 38-39 -0.5135984080571916 1212 40-41 -0.6304580092997938 1212 42-43 -0.08987424483049722 1212 44-45 0.7359123324049079 1212 46-47 -2.350582214111995 1212 48-49 -1.5365932667450153 1212 50-51 -0.196763475335608 1212 52-53 -0.9410029614563342 1212 54-55 -0.6803940626480873 1212 56-57 -1.4912502676140775 1212 58-59 -0.43489457476092497 1212 60-61 0.07367065670811712 1212 62-63 -4.86139414030816 1212 64-65 -3.991797081344753 1212 66-67 -1.421671128735099 1212 68-69 0.4402238753855485 1212 70-71 -0.7136592728227704 1212 72-73 -1.1450933688184932 1212 74-75 -0.4256060815588931 1212 76-77 0.7848424092900359 1212 78-79 -1.383741525369075 1212 80-81 0.4597754883530669 1212 82-83 0.48129642758182456 1212 84-85 -3.357644291408775 1212 86-87 -1.6696075467391722 1212 88-89 -0.798159723698344 1212 90-91 -0.1316983490075181 1212 92-93 -2.381975003891448 1212 94-95 -2.11104070023892 1212 96-97 -0.013919221577520347 1212 98-99 -0.6236612926617546 1212 100-101 0.09723637361302906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 12.0 10 12.0 11 19.0 12 108.0 13 502.0 14 1550.0 15 3810.0 16 7073.0 17 11476.0 18 17163.0 19 24582.0 20 34468.0 21 45687.0 22 60049.0 23 77298.0 24 97537.0 25 121270.0 26 149834.0 27 181586.0 28 217497.0 29 259498.0 30 307895.0 31 360028.0 32 418090.0 33 479444.0 34 541120.0 35 595576.0 36 630217.0 37 580521.0 38 207863.0 39 516.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.45594332180711 22.742451112389606 16.59778485892729 20.203820706875998 2 24.41822855332475 27.874865223937668 27.859088807967513 19.847817414770073 3 19.333481337322812 26.37609654572679 31.799651196568075 22.49077092038232 4 22.32754082064687 26.568967104173186 29.55930268946855 21.544189385711395 5 20.783251741158722 28.681487148542185 28.411325438092362 22.123935672206738 6 20.217598358854126 29.37962580136377 28.176194916998355 22.226580922783747 7 21.596295640411746 28.424027235599198 29.575343933382204 20.404333190606856 8 20.433068706415806 27.80040579481774 29.904375714015902 21.862149784750553 9 21.042644536331007 27.402563406820903 29.46583234879062 22.08895970805747 10-11 21.735776103758592 27.921174117344727 28.19854271724952 22.14450706164716 12-13 21.702311804800395 28.009545942545643 28.19574548837477 22.092396764279197 14-15 21.618122780751655 28.25172979185064 28.13656680658896 21.99358062080875 16-17 21.87350431126525 28.20709720412055 27.92067051035661 21.998727974257587 18-19 22.022885714161976 28.337323370480412 27.71507660898966 21.924714306367957 20-21 21.77783410749883 28.341489177422236 27.879705119432813 22.000971595646117 22-23 21.72458779438032 28.082409277394603 28.097126797649835 22.09587613057524 24-25 21.85602899102443 27.94829889796259 28.140335349544266 22.05533676146871 26-27 21.875367017518542 28.21543242625318 27.98581522161323 21.923385334615052 28-29 22.056818637844508 28.099735618527838 28.018674219511357 21.824771524116297 30-31 22.080657518672563 27.979252626234697 27.790437645035198 22.149652210057543 32-33 21.90963425818373 28.155991695601607 27.952164662421197 21.982209383793464 34-35 22.17986960224229 27.991392967474933 27.86929923999071 21.959438190292072 36-37 22.152312224173105 27.988972262587758 27.87234583055213 21.986369682687005 38-39 21.867736734813345 28.046719420238418 28.173102305431474 21.912441539516763 40-41 22.1454937292137 27.961944058347488 27.831297499628832 22.06126471280998 42-43 21.886780226872514 28.10157645874622 28.03468952941129 21.976953784969982 44-45 21.854547114648636 27.91309282878603 28.204884411801846 22.02747564476349 46-47 22.09461209908454 27.955378335526593 28.00676813405317 21.943241431335696 48-49 22.028574839388828 27.898155043635782 28.053660178526705 22.019609938448685 50-51 22.040557631770515 27.975683203478752 27.984951846774877 21.998807317975857 52-53 21.93668380431019 28.040222808387842 28.08797430034584 21.935119086956128 54-55 21.8519309413598 27.829486975338696 28.214349816234506 22.104232267066994 56-57 21.94183693521672 28.05701712603163 28.01232091255383 21.988825026197816 58-59 21.946198307164966 27.91903673828274 28.194075906535694 21.9406890480166 60-61 22.100190981009497 27.806840505499093 27.984751341326575 22.10821717216484 62-63 21.945612958505173 27.99574171314312 28.106211968322782 21.95243336002893 64-65 22.07827961534047 27.811152446057342 28.079009892988644 22.031558045613544 66-67 22.13085133428803 27.851485059202997 27.990106171253586 22.02755743525538 68-69 22.01399804051733 27.956114510075945 28.074053496601465 21.95583395280526 70-71 22.233102978147475 27.842571917383612 27.87555168393326 22.048773420535653 72-73 22.015501092258432 27.94584976324809 28.114294658867777 21.924354485625695 74-75 21.96271135717492 27.827502618584464 28.200969164009713 22.008816860230894 76-77 22.11169992015034 28.017717000344582 27.98764662534249 21.88293645416259 78-79 22.126629171102337 27.930427829015663 27.959642043949444 21.983300955932556 80-81 22.060129837723117 27.859924093622045 28.026321754729587 22.053624313925248 82-83 22.08112587764744 27.967920852191646 27.97958308484739 21.97137018531352 84-85 21.9960035231735 27.683946042684376 28.08444055353478 22.23560988060735 86-87 21.968649365628075 27.913966399712077 28.038001103655734 22.079383131004118 88-89 22.054964267758287 27.845052834821665 27.972318857628665 22.12766403979139 90-91 22.14833412061861 27.772322069307926 27.820645519917154 22.258698290156314 92-93 21.976796377144847 27.886700352631145 28.00899086075212 22.127512409471887 94-95 22.108624704032703 27.79804566077722 27.850986306038173 22.242343329151904 96-97 22.130960798141235 27.834690364771486 27.769472219049018 22.26487661803826 98-99 21.988794254740434 27.84358719349711 27.908861681417786 22.25875687034467 100-101 22.19084323836391 27.659197048839246 27.713508598853142 22.436451113943697 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 5.0 2 4.5 3 9.5 4 20.0 5 29.5 6 46.0 7 77.0 8 120.0 9 176.0 10 257.0 11 381.5 12 587.0 13 904.5 14 1373.5 15 2081.5 16 3131.0 17 4631.5 18 6882.0 19 10238.5 20 14732.5 21 20243.0 22 27236.5 23 35483.0 24 44911.5 25 55780.0 26 67454.0 27 80149.5 28 92889.0 29 105160.0 30 117386.5 31 128860.5 32 139841.5 33 150296.0 34 160486.5 35 169557.5 36 177267.5 37 183584.5 38 186793.0 39 186452.5 40 182973.0 41 176953.0 42 168859.5 43 159728.0 44 150351.0 45 142318.0 46 135269.5 47 129585.5 48 124154.0 49 117492.0 50 111473.0 51 105602.0 52 99823.5 53 94742.5 54 90633.5 55 87396.0 56 85195.5 57 83731.5 58 82029.0 59 80059.0 60 77878.0 61 75302.5 62 72282.5 63 69892.0 64 67367.5 65 64180.0 66 60714.5 67 56940.0 68 52939.5 69 48071.5 70 42858.5 71 37251.5 72 31508.0 73 25770.5 74 19999.5 75 14828.0 76 10555.5 77 7247.5 78 4685.5 79 2890.5 80 1782.0 81 1101.0 82 732.0 83 505.5 84 336.0 85 234.5 86 167.0 87 125.0 88 95.0 89 61.0 90 40.5 91 22.0 92 16.0 93 15.0 94 8.5 95 4.0 96 1.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6823994689544138 2 0.002632401512287056 3 2.945344349412091E-4 4 3.6816804367651134E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.18212352700567824 14-15 1.8408402183825567E-5 16-17 4.0498484804416245E-4 18-19 0.11189547267438371 20-21 1.8408402183825567E-5 22-23 1.8408402183825567E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0016291435932685627 42-43 0.0 44-45 0.0 46-47 0.023249811958171693 48-49 1.0124621201104061E-4 50-51 1.3806301637869176E-4 52-53 2.0249242402208123E-4 54-55 0.0019696990336693357 56-57 0.0013714259626950048 58-59 0.09342264108291476 60-61 0.0015923267889009114 62-63 0.0012977923539597026 64-65 0.0022366208653348063 66-67 0.004215524100096056 68-69 0.004712550959059345 70-71 0.0033779418007319918 72-73 0.037543936253912245 74-75 0.04352666696365555 76-77 0.15387583385459794 78-79 2.761260327573835E-4 80-81 0.1131012230174243 82-83 0.004371995518658573 84-85 0.0049242475841733396 86-87 0.006525778574166164 88-89 0.006866334014566936 90-91 0.021933611202028164 92-93 0.01038233883167762 94-95 0.013760280632409612 96-97 0.00978406576070329 98-99 0.011486842962707154 100-101 0.012453284077357997 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5432302.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.36805983170301 #Duplication Level Percentage of deduplicated Percentage of total 1 98.2127473796303 94.64571915716145 2 1.1810310622295963 2.27627344136083 3 0.22079477667585676 0.6383269274767941 4 0.12997497454155563 0.5010174449297884 5 0.06503535782203507 0.3133665626885045 6 0.04338883456833041 0.25087786834252374 7 0.03721781106405527 0.2510625770398128 8 0.026071644140330124 0.2009979009940962 9 0.023191848824375322 0.20114581276036908 >10 0.060488989052178845 0.7060214253310496 >50 0.0 0.0 >100 5.732145141835729E-5 0.01519088191479186 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.52252065514767E-5 1.8408402183825567E-5 0.0 2 5.52252065514767E-5 1.8408402183825567E-5 0.0 3 7.363360873530227E-5 1.8408402183825567E-5 0.0 4 1.104504131029534E-4 1.8408402183825567E-5 0.0 5 1.2885881528677898E-4 3.6816804367651134E-5 0.0 6 1.2885881528677898E-4 3.6816804367651134E-5 0.0 7 1.2885881528677898E-4 3.6816804367651134E-5 0.0 8 1.4726721747060454E-4 3.6816804367651134E-5 0.0 9 1.4726721747060454E-4 3.6816804367651134E-5 0.0 10-11 1.5647141856251731E-4 4.602100545956392E-5 4.602100545956392E-5 12-13 1.8408402183825567E-4 6.442940764338949E-5 9.204201091912784E-5 14-15 2.1169662511399403E-4 7.363360873530227E-5 1.196546141948662E-4 16-17 2.5771763057355797E-4 7.363360873530227E-5 1.2885881528677898E-4 18-19 2.761260327573835E-4 7.363360873530227E-5 1.2885881528677898E-4 20-21 2.853302338492963E-4 7.363360873530227E-5 1.3806301637869176E-4 22-23 3.2214703821694746E-4 7.363360873530227E-5 1.4726721747060454E-4 24-25 3.497596414926858E-4 7.363360873530227E-5 1.4726721747060454E-4 26-27 4.418016524118136E-4 8.283780982721505E-5 1.5647141856251731E-4 28-29 4.970268589632904E-4 9.204201091912784E-5 1.6567561965443012E-4 30-31 7.087234840772844E-4 9.204201091912784E-5 1.9328822293016848E-4 32-33 0.0010768915277537957 9.204201091912784E-5 2.0249242402208125E-4 34-35 0.0016107351910847373 1.0124621201104063E-4 2.0249242402208125E-4 36-37 0.0023654796806215853 1.196546141948662E-4 2.0249242402208125E-4 38-39 0.003543617420386422 1.2885881528677898E-4 2.1169662511399403E-4 40-41 0.005320028231125588 1.2885881528677898E-4 2.209008262059068E-4 42-43 0.007446198683357442 1.2885881528677898E-4 2.393092283897324E-4 44-45 0.010189050608747452 1.3806301637869176E-4 2.5771763057355797E-4 46-47 0.014340145301200119 1.8408402183825567E-4 2.6692183166547074E-4 48-49 0.020258446603300034 1.8408402183825567E-4 2.853302338492963E-4 50-51 0.028790741015503187 1.8408402183825567E-4 2.945344349412091E-4 52-53 0.03795812530304832 1.8408402183825567E-4 3.0373863603312185E-4 54-55 0.04964746068977756 2.209008262059068E-4 3.1294283712503463E-4 56-57 0.06547868656786755 2.209008262059068E-4 3.1294283712503463E-4 58-59 0.08409878537680711 2.209008262059068E-4 3.1294283712503463E-4 60-61 0.10604160077992719 2.209008262059068E-4 3.3135123930886024E-4 62-63 0.1312519075706763 2.393092283897324E-4 3.3135123930886024E-4 64-65 0.16071455526588913 2.485134294816452E-4 3.3135123930886024E-4 66-67 0.1956905194151577 2.761260327573835E-4 3.3135123930886024E-4 68-69 0.237026586518938 3.3135123930886024E-4 3.773722447684241E-4 70-71 0.28274385334246876 3.3135123930886024E-4 3.9578064695224973E-4 72-73 0.33402966182660687 3.3135123930886024E-4 4.2339325022798806E-4 74-75 0.39077356155824916 3.3135123930886024E-4 4.602100545956392E-4 76-77 0.45563556665295857 3.3135123930886024E-4 4.970268589632904E-4 78-79 0.5309995651935404 3.3135123930886024E-4 5.430478644228543E-4 80-81 0.6139202128305827 3.3135123930886024E-4 5.706604676985926E-4 82-83 0.7045447767815559 3.3135123930886024E-4 5.982730709743309E-4 84-85 0.8037660645523758 3.40555440400773E-4 6.442940764338949E-4 86-87 0.9159744800638845 3.497596414926858E-4 6.442940764338949E-4 88-89 1.04140012834338 3.6816804367651134E-4 6.534982775258076E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGGAT 2460 1.5825208E-10 5.826359 1 GTCGATT 1070 0.0018747642 5.8045783 1 AGTCACG 2110 0.0 5.7358875 94-95 ACGTCTG 4705 0.0 5.295934 94-95 TCGGAAG 8710 0.0 5.258381 94-95 CGTCTGA 4310 0.0 5.176967 90-91 GCGCTAA 1205 1.2915552E-7 5.1203446 94-95 TCACGCG 2285 0.0 5.0888867 94-95 CACGTCT 5065 0.0 5.0600777 94-95 GTCTCAT 2845 6.6338544E-9 5.037906 1 CGCGCTA 1515 1.4460966E-9 5.012452 94-95 >>END_MODULE