FastQCFastQC Report
Thu 11 Sep 2014
LONLib41_GCGCTA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib41_GCGCTA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5432302
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGGAT24601.5825208E-105.8263591
GTCGATT10700.00187476425.80457831
AGTCACG21100.05.735887594-95
ACGTCTG47050.05.29593494-95
TCGGAAG87100.05.25838194-95
CGTCTGA43100.05.17696790-91
GCGCTAA12051.2915552E-75.120344694-95
TCACGCG22850.05.088886794-95
CACGTCT50650.05.060077794-95
GTCTCAT28456.6338544E-95.0379061
CGCGCTA15151.4460966E-95.01245294-95
Produced by FastQC (version 0.11.2)