##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib40_CTCAGA_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3456037 Sequences flagged as poor quality 0 Sequence length 101 %GC 52 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.47426864932291 27.0 15.0 33.0 2.0 33.0 2 24.48138257779069 27.0 15.0 33.0 2.0 33.0 3 25.35418573354394 27.0 22.0 33.0 6.0 33.0 4 27.381462061893433 33.0 15.0 37.0 2.0 37.0 5 27.427535932051654 33.0 15.0 37.0 6.0 37.0 6 28.457569464678762 33.0 22.0 37.0 15.0 37.0 7 28.112313901731955 33.0 22.0 37.0 6.0 37.0 8 27.860558495178147 33.0 22.0 37.0 6.0 37.0 9 28.57850335514348 33.0 22.0 37.0 6.0 37.0 10-11 28.1457358529437 33.0 22.0 37.0 6.0 37.0 12-13 28.403932162763304 33.0 22.0 37.0 10.5 37.0 14-15 28.614521343376822 33.0 22.0 37.0 6.0 40.0 16-17 29.388905269243356 33.0 24.5 37.0 15.0 40.0 18-19 29.61727406274875 33.0 27.0 37.0 10.5 40.0 20-21 29.562071818096854 33.0 27.0 37.0 10.5 40.0 22-23 29.49901534618987 33.0 27.0 37.0 10.5 40.0 24-25 29.022918012741183 33.0 24.5 37.0 10.5 40.0 26-27 28.423421103419898 33.0 22.0 37.0 6.0 40.0 28-29 28.500740443461687 33.0 22.0 37.0 6.0 40.0 30-31 28.312128168766712 33.0 22.0 37.0 6.0 40.0 32-33 28.06231921128159 33.0 22.0 37.0 6.0 40.0 34-35 28.039603308645134 33.0 22.0 37.0 6.0 40.0 36-37 28.25568027772851 33.0 22.0 37.0 6.0 40.0 38-39 28.194734749656906 33.0 22.0 37.0 6.0 40.0 40-41 28.163069145382416 33.0 22.0 37.0 6.0 40.0 42-43 27.95790149237407 33.0 22.0 37.0 6.0 40.0 44-45 27.596487826953243 33.0 22.0 37.0 6.0 40.0 46-47 27.161258690228145 33.0 22.0 37.0 6.0 40.0 48-49 26.910233165906497 30.0 22.0 37.0 6.0 37.0 50-51 26.053451395340964 27.0 18.5 35.0 6.0 37.0 52-53 25.760160843185417 27.0 15.0 35.0 6.0 37.0 54-55 26.591269566847807 27.0 22.0 37.0 6.0 37.0 56-57 26.425113938305635 27.0 22.0 37.0 6.0 37.0 58-59 26.56126728388614 27.0 22.0 37.0 6.0 37.0 60-61 26.28879971481787 27.0 18.5 35.0 6.0 37.0 62-63 26.09126797542966 27.0 18.5 33.0 6.0 37.0 64-65 26.020178169388814 27.0 18.5 33.0 6.0 37.0 66-67 25.864583770370515 27.0 18.5 33.0 6.0 37.0 68-69 25.53588387508583 27.0 15.0 33.0 6.0 37.0 70-71 25.41681107580735 27.0 15.0 33.0 6.0 37.0 72-73 25.176752013939666 27.0 15.0 33.0 6.0 37.0 74-75 25.09342507039132 27.0 15.0 33.0 6.0 37.0 76-77 24.722820097122803 27.0 15.0 33.0 6.0 37.0 78-79 24.176902619966164 27.0 15.0 33.0 6.0 37.0 80-81 24.053574513235823 27.0 15.0 33.0 6.0 37.0 82-83 23.655918903645997 27.0 15.0 33.0 6.0 37.0 84-85 23.737919617180026 27.0 15.0 33.0 6.0 37.0 86-87 23.457031565344934 27.0 15.0 33.0 6.0 37.0 88-89 23.105947939793467 27.0 15.0 33.0 6.0 37.0 90-91 22.958506809967602 27.0 15.0 33.0 6.0 37.0 92-93 22.833479502678934 27.0 15.0 33.0 6.0 33.0 94-95 22.621694298990434 27.0 15.0 33.0 6.0 33.0 96-97 22.490608028791357 27.0 15.0 33.0 6.0 33.0 98-99 22.236381873226474 27.0 15.0 33.0 6.0 33.0 100-101 21.76684147189396 24.5 15.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.196037024904566 2306 2 8.30023208561174 2306 3 7.3023489744616406 2306 4 8.909409429137188 2306 5 8.767233880022843 2306 6 7.7639249271354664 2306 7 7.94039225218884 2306 8 8.349132614981635 2306 9 7.29454906801546 2306 10-11 7.614643351404997 2306 12-13 7.176588698245851 2306 14-15 7.6860016807628355 2306 16-17 6.508051290047714 2306 18-19 5.860172519031575 2306 20-21 5.634002345706474 2306 22-23 5.363303359514024 2306 24-25 5.364796467530287 2306 26-27 5.802713238537631 2306 28-29 4.858584592347899 2306 30-31 4.813750220539781 2306 32-33 4.763846383886083 2306 34-35 4.090693188828755 2306 36-37 4.072432419526425 2306 38-39 3.694949782623926 2306 40-41 2.8819593842325766 2306 42-43 2.712783928039805 2306 44-45 2.3546188800279495 2306 46-47 2.5925656303999993 2306 48-49 2.43144363069975 2306 50-51 2.1846769676599322 2306 52-53 2.7952665570319866 2306 54-55 2.3661409452455118 2306 56-57 2.5625534659628677 2306 58-59 2.01283020722925 2306 60-61 2.167662171548809 2306 62-63 1.7373728453016284 2306 64-65 1.3137742756096102 2306 66-67 1.2690721664199138 2306 68-69 1.35038882725949 2306 70-71 1.4235543640380257 2306 72-73 0.5466208270099244 2306 74-75 0.3020824077675641 2306 76-77 0.6402380717671399 2306 78-79 0.9000902047768236 2306 80-81 0.6311201523078012 2306 82-83 0.6238500735895265 2306 84-85 0.3080177807028939 2306 86-87 0.1005603853023942 2306 88-89 0.02831857400325788 2306 90-91 0.030216675240318125 2306 92-93 -0.13314627317452477 2306 94-95 0.1913408090455988 2306 96-97 -0.06932716418619833 2306 98-99 -0.16559767923286017 2306 100-101 -0.3348951177978421 2307 1 8.250196486340226 2307 2 8.35565272708465 2307 3 7.45087247904436 2307 4 9.094884793927495 2307 5 8.954646337116799 2307 6 7.492130946943302 2307 7 8.139523841830243 2307 8 8.523120629225176 2307 9 7.519457807539897 2307 10-11 7.73238149361622 2307 12-13 7.221202881333156 2307 14-15 7.979320709478614 2307 16-17 6.691722909193697 2307 18-19 6.260515732684329 2307 20-21 6.147157301638739 2307 22-23 5.879832220173103 2307 24-25 5.952800022515785 2307 26-27 6.614098388456757 2307 28-29 5.772773926211496 2307 30-31 5.520407853989681 2307 32-33 5.672790396334779 2307 34-35 5.330290258082606 2307 36-37 5.109565846349909 2307 38-39 4.718616185894071 2307 40-41 4.096253486753167 2307 42-43 3.7032483839108252 2307 44-45 3.7796712383291187 2307 46-47 4.017379873384858 2307 48-49 3.8069919483455443 2307 50-51 3.465850675064914 2307 52-53 4.047989535723939 2307 54-55 3.725884100666466 2307 56-57 3.996934052077396 2307 58-59 3.6244644906987418 2307 60-61 3.511573165733612 2307 62-63 3.2393152124763844 2307 64-65 3.0614204164340926 2307 66-67 2.8091104161760363 2307 68-69 2.8407310067598814 2307 70-71 2.5606597681087706 2307 72-73 2.033280903558019 2307 74-75 1.748936276833465 2307 76-77 1.8703581757237444 2307 78-79 2.2436324264761893 2307 80-81 1.9920769792229684 2307 82-83 2.125270939938517 2307 84-85 1.451931082643803 2307 86-87 1.4187082496563086 2307 88-89 1.5455962754920591 2307 90-91 1.2997753246928596 2307 92-93 1.2105399143325712 2307 94-95 1.3559782119766943 2307 96-97 1.1776111028173695 2307 98-99 0.850426181924206 2307 100-101 0.6642089175826627 1101 1 7.923771226520383 1101 2 7.590533927316834 1101 3 7.1746447625019165 1101 4 8.586108558897802 1101 5 8.49247929283976 1101 6 7.329793227253024 1101 7 6.997557121239421 1101 8 7.899570071889222 1101 9 7.208604674245262 1101 10-11 7.387146432484318 1101 12-13 7.050212048909849 1101 14-15 7.545682002230933 1101 16-17 6.694478145954161 1101 18-19 5.8178637490728455 1101 20-21 5.674294091746841 1101 22-23 5.128130606440781 1101 24-25 5.784265718382866 1101 26-27 6.142586609168223 1101 28-29 5.49696834073406 1101 30-31 5.295745380429182 1101 32-33 5.228674228946396 1101 34-35 5.003642265258673 1101 36-37 4.911241475448318 1101 38-39 4.537032755434851 1101 40-41 3.960834081336703 1101 42-43 3.7162272757677606 1101 44-45 3.7147175319593693 1101 46-47 3.97797732918713 1101 48-49 3.6635554266334474 1101 50-51 3.0954005032059726 1101 52-53 4.060657795502443 1101 54-55 3.893690199124027 1101 56-57 4.079502513903652 1101 58-59 3.5841637366674135 1101 60-61 3.7982080019597575 1101 62-63 3.4264003548124684 1101 64-65 3.217015627065077 1101 66-67 2.779181487947678 1101 68-69 2.982892991894058 1101 70-71 2.808189267115843 1101 72-73 2.3416763956921507 1101 74-75 1.8629792844938962 1101 76-77 2.1584089890198186 1101 78-79 2.63296258112274 1101 80-81 2.101433867482683 1101 82-83 2.3578767094104762 1101 84-85 1.657886810197656 1101 86-87 1.7693195686405687 1101 88-89 1.8411391070363763 1101 90-91 1.805152465775464 1101 92-93 1.4633852229137858 1101 94-95 1.7571747769882684 1101 96-97 1.6242520898589667 1101 98-99 1.4450111149347222 1101 100-101 1.0506190070397565 2201 1 6.963100809750728 2201 2 7.680073552972065 2201 3 6.220563237889486 2201 4 8.361740879397257 2201 5 3.7285844174407323 2201 6 5.6019785813516165 2201 7 6.526996212673144 2201 8 7.790318222239989 2201 9 6.588652022987933 2201 10-11 3.8177364998185475 2201 12-13 6.305868442387009 2201 14-15 6.860791781806938 2201 16-17 5.819102454999378 2201 18-19 1.732435757047103 2201 20-21 4.8392879818585435 2201 22-23 5.006894460459026 2201 24-25 5.31414014491909 2201 26-27 5.99548944228081 2201 28-29 4.9367086664773225 2201 30-31 5.0684428013569764 2201 32-33 5.190470868872623 2201 34-35 4.751155886830684 2201 36-37 4.761205689849433 2201 38-39 4.115436030840357 2201 40-41 3.8383418804084855 2201 42-43 3.6974185536197126 2201 44-45 3.504305506432189 2201 46-47 3.8308291442430296 2201 48-49 3.6273778232934113 2201 50-51 3.2298558334006344 2201 52-53 4.128704138475825 2201 54-55 3.6774207953128197 2201 56-57 3.792679675395643 2201 58-59 3.558041139227285 2201 60-61 3.560847685466296 2201 62-63 3.250898382155796 2201 64-65 3.1895117339798453 2201 66-67 2.7704941200861377 2201 68-69 3.0305415968874208 2201 70-71 2.897208800429638 2201 72-73 2.2956806013452784 2201 74-75 1.8609364179375234 2201 76-77 2.161447633017886 2201 78-79 2.459252869060677 2201 80-81 2.1169702804417305 2201 82-83 2.352120005927887 2201 84-85 1.9045602620701167 2201 86-87 1.7752189963379053 2201 88-89 1.8909680177074435 2201 90-91 1.7709533024573574 2201 92-93 1.596054734277537 2201 94-95 1.7460180515467378 2201 96-97 1.6624348700992648 2201 98-99 1.5192895172847933 2201 100-101 1.2347523736140147 2304 1 1.0945805975685445 2304 2 4.367512123790917 2304 3 5.4072908764481085 2304 4 3.983149330101483 2304 5 6.314753553469021 2304 6 6.164688172230225 2304 7 6.907197419916603 2304 8 6.265355110234623 2304 9 5.684046206269443 2304 10-11 6.552595349888666 2304 12-13 6.428547547319631 2304 14-15 6.625724407866329 2304 16-17 5.336480663645194 2304 18-19 5.2442911317193825 2304 20-21 4.875626010027556 2304 22-23 4.539374187304947 2304 24-25 4.658684989745627 2304 26-27 5.469530049560856 2304 28-29 4.869827378750966 2304 30-31 4.817108126642019 2304 32-33 4.835330328661662 2304 34-35 4.512092415091153 2304 36-37 4.659110695641761 2304 38-39 4.2546933931963125 2304 40-41 3.6303240766160307 2304 42-43 2.940901268088343 2304 44-45 2.73385492415062 2304 46-47 3.9505828177995177 2304 48-49 3.4990173574681585 2304 50-51 3.10980949476275 2304 52-53 3.673119722891407 2304 54-55 3.523696022433114 2304 56-57 4.325283784901163 2304 58-59 3.465943096425015 2304 60-61 3.558350276771705 2304 62-63 3.351520295453767 2304 64-65 3.241562218706388 2304 66-67 2.9946292337987153 2304 68-69 3.137789935403365 2304 70-71 3.1653924477682125 2304 72-73 2.7927746809745706 2304 74-75 2.1907699646724765 2304 76-77 2.5255876244818225 2304 78-79 2.862975812783624 2304 80-81 2.691582804068343 2304 82-83 2.8744003546871113 2304 84-85 2.4524841691489527 2304 86-87 2.141877126939697 2304 88-89 2.361290559015899 2304 90-91 2.3856036286569804 2304 92-93 2.19168914681336 2304 94-95 2.3087166674847914 2304 96-97 2.2981436683432754 2304 98-99 2.0804699331130934 2304 100-101 1.6569961392380606 1102 1 6.9703221738381025 1102 2 7.344080827878102 1102 3 6.066965126872095 1102 4 5.996150788340536 1102 5 6.33438026255719 1102 6 5.768358911909409 1102 7 6.73062176748034 1102 8 6.403195910757578 1102 9 4.844101793062094 1102 10-11 5.884453804703824 1102 12-13 5.803807219010139 1102 14-15 6.131008718768378 1102 16-17 5.206005570276755 1102 18-19 5.351687557890887 1102 20-21 5.52646022333273 1102 22-23 5.200376578085585 1102 24-25 1.660400317712277 1102 26-27 5.381644953312506 1102 28-29 5.011203151869083 1102 30-31 4.903500242094324 1102 32-33 4.955881991008464 1102 34-35 5.009253008809299 1102 36-37 4.988365622341249 1102 38-39 3.9393781347131522 1102 40-41 3.967594198257853 1102 42-43 4.116871774175003 1102 44-45 -3.707586401155538 1102 46-47 1.0546400136835352 1102 48-49 -0.7998719123609241 1102 50-51 2.463856953815487 1102 52-53 -0.5480394628717882 1102 54-55 2.41267639791824 1102 56-57 -0.575783975216229 1102 58-59 2.6923907072443285 1102 60-61 3.718363990618574 1102 62-63 3.555168635849732 1102 64-65 3.064747945334563 1102 66-67 2.9714940655526725 1102 68-69 3.3427426454880695 1102 70-71 3.1647992781782115 1102 72-73 2.6495089569063097 1102 74-75 2.497028507256257 1102 76-77 2.7448376599092583 1102 78-79 -1.4707717701508685 1102 80-81 1.4278936878343451 1102 82-83 2.5905948536059995 1102 84-85 2.347581926020734 1102 86-87 2.426250564132566 1102 88-89 2.378918246358481 1102 90-91 2.2477179381419354 1102 92-93 2.1902199366852244 1102 94-95 2.449395868607496 1102 96-97 2.507126603746485 1102 98-99 2.274046641515568 1102 100-101 2.036286676226595 2202 1 -20.235289532301586 2202 2 -7.338592042415348 2202 3 -0.924352008932722 2202 4 1.4653425613361222 2202 5 0.9918361532288777 2202 6 1.5276463333732941 2202 7 3.6340634221314403 2202 8 2.413046005083771 2202 9 2.8775355605528965 2202 10-11 1.756516352312218 2202 12-13 1.1536980557259575 2202 14-15 2.503001972426933 2202 16-17 1.4924184713470403 2202 18-19 1.2667473935517073 2202 20-21 0.9401145711922361 2202 22-23 1.24180142541001 2202 24-25 2.226634053349823 2202 26-27 3.2424551845506535 2202 28-29 -1.229214838249277 2202 30-31 0.8108503949617116 2202 32-33 1.4469920720987872 2202 34-35 1.479082075081383 2202 36-37 2.8776180608422663 2202 38-39 2.3798252399844237 2202 40-41 1.8393717693279363 2202 42-43 0.6104460473778772 2202 44-45 0.6831321056569593 2202 46-47 -1.2840552081941503 2202 48-49 0.590029627482128 2202 50-51 1.6987024924816119 2202 52-53 1.6501070501793613 2202 54-55 -0.5792443610631395 2202 56-57 1.041185153337981 2202 58-59 1.904294686223004 2202 60-61 2.0269913426447594 2202 62-63 -1.9953623548888935 2202 64-65 0.010876527011053128 2202 66-67 0.5121873119466969 2202 68-69 1.380777365821313 2202 70-71 -2.5814014125593943 2202 72-73 -4.49256028279488 2202 74-75 -1.3640907730016458 2202 76-77 -0.19446865502993305 2202 78-79 1.4701758088548615 2202 80-81 1.5047557948136507 2202 82-83 -0.6250637622131947 2202 84-85 -0.3825554745123583 2202 86-87 0.5040407451328122 2202 88-89 1.202809762066586 2202 90-91 1.3803431640317996 2202 92-93 1.3818262360429046 2202 94-95 1.6574464251538537 2202 96-97 1.2703720374406196 2202 98-99 1.3061712539136785 2202 100-101 1.025475536566013 2305 1 5.537461651285998 2305 2 6.974870565804803 2305 3 6.656300579702947 2305 4 7.646771984844602 2305 5 7.833482224412315 2305 6 5.962453759264616 2305 7 2.5457215049846127 2305 8 5.771252257099679 2305 9 6.391487129365284 2305 10-11 4.621400285090228 2305 12-13 5.1582692932223395 2305 14-15 5.59272477905397 2305 16-17 5.107118081636639 2305 18-19 4.409006808818017 2305 20-21 5.086508001961622 2305 22-23 5.051054528706491 2305 24-25 5.234814709578661 2305 26-27 6.0766601770171285 2305 28-29 5.136023616320163 2305 30-31 5.4718422264892155 2305 32-33 5.235943494209071 2305 34-35 5.330522177508293 2305 36-37 4.6877651518290975 2305 38-39 4.5516962010144795 2305 40-41 3.8700329561986635 2305 42-43 3.730066095895989 2305 44-45 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30-31 -24.39051092097703 1209 32-33 -24.01462638129505 1209 34-35 -21.16345989776453 1209 36-37 -24.35361657708551 1209 38-39 -24.541237342734426 1209 40-41 -21.123470692909073 1209 42-43 -21.587738581450385 1209 44-45 -24.313937283641586 1209 46-47 -17.744195786866726 1209 48-49 -17.521741110031883 1209 50-51 -19.931673391888676 1209 52-53 -22.23796252819084 1209 54-55 -19.167137189298973 1209 56-57 -22.91731361769624 1209 58-59 -23.135788505306586 1209 60-61 -22.840091281669853 1209 62-63 -22.912548968615496 1209 64-65 -22.76441180726764 1209 66-67 -10.988018754916688 1209 68-69 -22.38723170961828 1209 70-71 -22.211577249201483 1209 72-73 -12.745483989194215 1209 74-75 -13.80659209898172 1209 76-77 -11.136510402335308 1209 78-79 -11.724606592326872 1209 80-81 -21.263915031429494 1209 82-83 -13.18069862872648 1209 84-85 -10.958675582460234 1209 86-87 -11.055457698035575 1209 88-89 -8.739792827011305 1209 90-91 -11.342197010154894 1209 92-93 -14.922427947079015 1209 94-95 -19.957603678835557 1209 96-97 -15.894192534779446 1209 98-99 -7.753530423583891 1209 100-101 -4.034419461391021 1212 1 -20.235289532301586 1212 2 -20.01811558183679 1212 3 -20.91357492441769 1212 4 -22.803517336565182 1212 5 -22.9673243686089 1212 6 -23.99721658967454 1212 7 -23.54423115151197 1212 8 -23.16702584214633 1212 9 -24.059503577280342 1212 10-11 -23.70982889253558 1212 12-13 -23.95482907605699 1212 14-15 -24.190985548843628 1212 16-17 -25.15581370864918 1212 18-19 -19.76032372078207 1212 20-21 -25.404373989972445 1212 22-23 -25.524695076764313 1212 24-25 -21.617557255981154 1212 26-27 -24.127179907149102 1212 28-29 -18.975513809270634 1212 30-31 -13.919720542970156 1212 32-33 -24.01462638129505 1212 34-35 -20.124645657832485 1212 36-37 -24.35361657708551 1212 38-39 -24.541237342734426 1212 40-41 -19.05836361026753 1212 42-43 -15.175132560625897 1212 44-45 -12.354315290514439 1212 46-47 -15.647969420340349 1212 48-49 -18.08713295270277 1212 50-51 -10.441419585435403 1212 52-53 -3.7847838340327655 1212 54-55 -2.4441073425867472 1212 56-57 -2.796609150342288 1212 58-59 -1.5825239004955876 1212 60-61 -12.310452106412122 1212 62-63 -9.83608848751584 1212 64-65 -14.028587064999597 1212 66-67 -10.237936705416708 1212 68-69 -5.974860575597663 1212 70-71 -13.098175187345813 1212 72-73 -9.686171853590523 1212 74-75 -6.7018551078495 1212 76-77 -16.503332802170565 1212 78-79 -8.067821259559839 1212 80-81 -5.947763828680355 1212 82-83 -4.157406223614316 1212 84-85 -3.096874290261052 1212 86-87 -1.9353340124363854 1212 88-89 -1.8822581088111185 1212 90-91 -2.05717474417294 1212 92-93 -1.9592134729036914 1212 94-95 -1.898754881584697 1212 96-97 -11.036133343304872 1212 98-99 -8.234068603951215 1212 100-101 -4.815731802922869 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 611.0 3 1295.0 4 917.0 5 4386.0 6 4840.0 7 6912.0 8 15296.0 9 25728.0 10 34503.0 11 44130.0 12 55619.0 13 66751.0 14 74730.0 15 80374.0 16 86482.0 17 93721.0 18 101639.0 19 108474.0 20 114808.0 21 122619.0 22 128948.0 23 134785.0 24 136994.0 25 139108.0 26 140498.0 27 141185.0 28 139755.0 29 140639.0 30 142518.0 31 147659.0 32 154415.0 33 164209.0 34 174294.0 35 175682.0 36 169315.0 37 138756.0 38 43327.0 39 115.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.478487018215844 22.023179545214848 11.002666836308482 20.495666600260826 2 26.870157968069986 24.315763394360378 25.292133288177027 23.521945349392613 3 21.99708243419871 22.873216496251246 28.0151621227356 27.11453894681445 4 27.338354955356937 22.132712925519005 24.23741721226047 26.29151490686359 5 25.25084447181505 24.99124434063125 24.27781317506452 25.480098012489183 6 24.46261916984285 25.093651716085436 24.01704252049008 26.426686593581632 7 27.510111716335985 24.51464882176453 24.297254524538822 23.677984937360666 8 24.159099493551846 24.218165840572894 25.175496748299636 26.447237917575627 9 25.541288003694206 24.270930205195423 24.32126409891481 25.866517692195558 10-11 26.412558129487966 24.394990449328986 23.37390182712514 25.81854959405791 12-13 25.978161895938655 24.303420561735393 23.693093601081795 26.025323941244157 14-15 25.65438772611161 24.743361004844154 24.00975254689858 25.592498722145663 16-17 26.121154994990025 24.620271082377805 23.66137962851014 25.597194294122033 18-19 26.287200128496103 24.68199902194883 23.37310920556331 25.657691643991765 20-21 25.766771792946226 24.89625460595543 23.835824023231687 25.501149577866656 22-23 26.163374653337478 24.3615202960574 23.679952089404466 25.79515296120065 24-25 26.000620976700795 24.357414178728355 23.8824104380402 25.75955440653065 26-27 25.689095878152457 24.61484276273226 24.031120946628214 25.664940412487073 28-29 26.275774612526337 24.45202113815132 23.64916402346571 25.623040225856634 30-31 26.160875090035624 24.369602671387288 23.64581095389525 25.82371128468184 32-33 25.799100888924748 24.615801751123442 23.913198779370884 25.671898580580926 34-35 26.258114732452775 24.436889711377606 23.66906136058115 25.635934195588465 36-37 26.11913661764816 24.331147271899784 23.713454350113032 25.836261760339024 38-39 25.843689974847265 24.57398500807313 24.018888672354148 25.563436344725453 40-41 26.341700776938577 24.419461952839285 23.62011815788434 25.618719112337796 42-43 26.189314078308957 24.37201519456636 23.65054825057505 25.788122476549635 44-45 25.859054129664642 24.530996701801918 23.982630603866568 25.62731856466687 46-47 26.367599124614554 24.412044703079275 23.524913350097282 25.695442822208886 48-49 26.191027958526576 24.250923601121503 23.709697738532256 25.848350701819662 50-51 25.99819537292442 24.509304557271193 23.82320826836627 25.669291801438117 52-53 26.24421843706985 24.350217266375633 23.72734448715113 25.67821980940339 54-55 26.117669415777694 24.267385358976647 23.69847626525521 25.916468959990453 56-57 25.77811359980507 24.506234322487654 24.032294110168447 25.68335796753883 58-59 26.351426419939017 24.336653446174846 23.579355575973153 25.732564557912983 60-61 26.185515551331235 24.193235535520348 23.64303847168493 25.97821044146349 62-63 25.953700338243692 24.446953295819924 23.881195342173154 25.71815102376323 64-65 26.362721957349688 24.33058610753937 23.55935382718967 25.74733810792127 66-67 26.322295183953475 24.203637430211977 23.592389348136866 25.881678037697682 68-69 26.067350051497424 24.39329737215849 23.827569540478493 25.711783035865597 70-71 26.518944817212258 24.273541142256438 23.414699297220885 25.792814743310416 72-73 26.33621034170797 24.187301122114352 23.5174873534805 25.95900118269718 74-75 26.186850353137935 24.369870332401664 23.626417472228415 25.816861842231987 76-77 26.628352406899648 24.294705310530215 23.25867691403082 25.81826536853932 78-79 26.52705990882963 24.166439202967197 23.355700191653373 25.950800696549802 80-81 26.38381130387353 24.36412754929028 23.497951803575127 25.75410934326106 82-83 26.701958360491602 24.149275201368486 23.35777044254261 25.79099599559731 84-85 26.713894565891362 24.06391951703504 23.197600173376788 26.024585743696814 86-87 26.518987607581572 24.293809120174643 23.442511914004545 25.74469135823924 88-89 26.91773688434641 24.136095172332112 23.159386551230796 25.78678139209068 90-91 26.929614811916597 24.035769998953068 23.007533124941446 26.02708206418889 92-93 26.777142355295574 24.25196236468368 23.175808427005453 25.795086853015288 94-95 27.16250360617712 24.153385882388847 22.843665954272584 25.84044455716145 96-97 27.140398203758885 24.067676459373303 22.894683269186704 25.897242067681102 98-99 27.058950414652877 24.221533285511505 22.956416750572018 25.7630995492636 100-101 27.798162782978793 23.898930588368525 22.39002936165764 25.91287726699504 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 218.0 1 226.5 2 254.0 3 285.0 4 312.5 5 347.0 6 406.5 7 524.5 8 692.0 9 894.5 10 1136.0 11 1482.5 12 1945.5 13 2536.0 14 3286.5 15 4162.0 16 5164.5 17 6447.5 18 7901.0 19 9566.5 20 11473.5 21 13617.0 22 16211.5 23 19162.5 24 22292.0 25 25593.0 26 29348.0 27 33363.5 28 37409.0 29 41508.0 30 45544.0 31 49746.0 32 53892.5 33 57500.5 34 61257.5 35 64495.5 36 67046.5 37 69555.5 38 71130.0 39 71807.0 40 72187.5 41 71958.0 42 71194.5 43 70725.0 44 70038.5 45 69146.0 46 68437.5 47 67801.5 48 66673.5 49 65576.0 50 64532.5 51 63075.0 52 61762.0 53 61073.0 54 60557.0 55 60046.5 56 60415.5 57 61016.5 58 62121.5 59 63959.0 60 65865.5 61 68315.5 62 70894.5 63 73322.0 64 75640.5 65 77713.0 66 78909.5 67 78660.0 68 77606.0 69 75173.5 70 70942.5 71 65487.5 72 59763.0 73 53281.5 74 45941.5 75 38850.0 76 32155.5 77 25985.0 78 20684.0 79 16469.5 80 13153.5 81 10442.5 82 8311.0 83 6600.5 84 5238.0 85 4167.5 86 3296.5 87 2649.0 88 2105.5 89 1662.5 90 1292.5 91 1001.0 92 787.0 93 585.5 94 430.5 95 305.5 96 192.0 97 113.5 98 62.0 99 34.0 100 15.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.58064164243612 2 10.890045448008804 3 9.61216561049549 4 11.54857427741659 5 9.037981942901654 6 5.248902138489837 7 9.626054350691268 8 9.912770031107884 9 5.320776369002994 10-11 6.233353404491908 12-13 5.272455127071845 14-15 7.218962065510294 16-17 2.5970208073582546 18-19 3.0821139935712494 20-21 3.0011108098669084 22-23 3.032230268368076 24-25 3.4070092420885536 26-27 4.290955218361377 28-29 1.6922127859163545 30-31 2.2227481939574143 32-33 2.994860876778808 34-35 1.9894607609814363 36-37 3.0457428551835526 38-39 1.338773282809183 40-41 1.1406706583291788 42-43 0.5541173314984765 44-45 1.2781691862673923 46-47 1.340813191525438 48-49 1.2374578165685146 50-51 1.5529926328913723 52-53 2.4212414392554247 54-55 1.791343669063728 56-57 1.7952209423683831 58-59 1.5550470090453314 60-61 1.3963392174331468 62-63 0.6101641851635269 64-65 1.2008407317398513 66-67 0.5607578854045834 68-69 0.8017853975521674 70-71 0.9935078820047355 72-73 0.08428729206313475 74-75 0.10510593491909953 76-77 0.44972030102686983 78-79 0.8848863597235794 80-81 0.29515019659801095 82-83 0.4723039712827149 84-85 0.06618852749551003 86-87 0.10076570360791856 88-89 0.08120572783219623 90-91 0.08933642782180862 92-93 0.17148253910476075 94-95 0.4559991689903783 96-97 0.36527386714899174 98-99 0.06494432785297148 100-101 0.22663241163216713 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3456037.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.6309212659475 #Duplication Level Percentage of deduplicated Percentage of total 1 98.91259161723053 97.55840036009884 2 0.8817925386877418 1.7394402091242127 3 0.14819418135845766 0.4384958590091276 4 0.0349719307038327 0.13797254975071582 5 0.012731784352229013 0.06278738100098613 6 0.00424883810909496 0.025144009020594136 7 0.005469109558120156 0.03775963199552531 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.1573950163149295E-4 0.0 0.0 2 1.446743770393662E-4 0.0 0.0 3 1.7360925244723943E-4 0.0 0.0 4 2.893487540787324E-4 0.0 2.8934875407873237E-5 5 3.4721850489447887E-4 0.0 2.8934875407873237E-5 6 3.4721850489447887E-4 0.0 2.8934875407873237E-5 7 4.050882557102253E-4 0.0 2.8934875407873237E-5 8 5.497626327495915E-4 0.0 2.8934875407873237E-5 9 5.786975081574648E-4 0.0 5.7869750815746473E-5 10-11 6.07632383565338E-4 0.0 7.23371885196831E-5 12-13 6.655021343810845E-4 0.0 8.680462622361972E-5 14-15 7.667741983086408E-4 2.8934875407873237E-5 8.680462622361972E-5 16-17 8.391113868283238E-4 2.8934875407873237E-5 8.680462622361972E-5 18-19 8.535788245322604E-4 2.8934875407873237E-5 8.680462622361972E-5 20-21 8.680462622361971E-4 4.340231311180985E-5 1.1573950163149295E-4 22-23 8.825136999401338E-4 5.7869750815746473E-5 1.7360925244723943E-4 24-25 9.259160130519436E-4 7.23371885196831E-5 1.7360925244723943E-4 26-27 9.8378576386769E-4 1.1573950163149295E-4 1.7360925244723943E-4 28-29 0.0013599391441700421 1.1573950163149295E-4 2.0254412785511265E-4 30-31 0.00196757152773538 1.1573950163149295E-4 2.314790032629859E-4 32-33 0.0027922154768597676 1.1573950163149295E-4 2.314790032629859E-4 34-35 0.004311296435773112 1.1573950163149295E-4 2.314790032629859E-4 36-37 0.0063078028389163664 1.1573950163149295E-4 2.314790032629859E-4 38-39 0.009389367069854864 1.446743770393662E-4 2.314790032629859E-4 40-41 0.012933889307319337 1.446743770393662E-4 2.314790032629859E-4 42-43 0.017447729870947563 1.7360925244723943E-4 2.6041387867085914E-4 44-45 0.02358192345741669 2.0254412785511265E-4 2.6041387867085914E-4 46-47 0.030974784124128303 2.314790032629859E-4 2.6041387867085914E-4 48-49 0.04058116275954221 2.314790032629859E-4 2.7488131637479576E-4 50-51 0.05182236185550097 2.6041387867085914E-4 2.893487540787324E-4 52-53 0.06636213674795727 2.6041387867085914E-4 3.182836294866056E-4 54-55 0.08463451056802922 2.6041387867085914E-4 3.182836294866056E-4 56-57 0.10429575840767909 2.6041387867085914E-4 3.182836294866056E-4 58-59 0.12961377438956817 2.6041387867085914E-4 3.182836294866056E-4 60-61 0.1576082663466855 2.6041387867085914E-4 3.182836294866056E-4 62-63 0.19175141932797596 2.6041387867085914E-4 3.182836294866056E-4 64-65 0.23084243600401266 2.6041387867085914E-4 3.182836294866056E-4 66-67 0.2744906955567895 2.6041387867085914E-4 3.182836294866056E-4 68-69 0.323448504746911 2.7488131637479576E-4 3.182836294866056E-4 70-71 0.3790902701562512 2.893487540787324E-4 3.182836294866056E-4 72-73 0.43682981403266224 2.893487540787324E-4 3.182836294866056E-4 74-75 0.5016150000708904 2.893487540787324E-4 3.182836294866056E-4 76-77 0.570653612794076 2.893487540787324E-4 3.182836294866056E-4 78-79 0.6516423290607132 2.893487540787324E-4 3.182836294866056E-4 80-81 0.7414995846398635 2.893487540787324E-4 3.182836294866056E-4 82-83 0.839067984515212 2.893487540787324E-4 3.182836294866056E-4 84-85 0.9434216126737069 2.893487540787324E-4 3.182836294866056E-4 86-87 1.063472410740973 2.893487540787324E-4 3.182836294866056E-4 88-89 1.189917816273379 2.893487540787324E-4 3.182836294866056E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATACG 850 2.093837E-4 7.6658883 3 ATGATAC 1120 9.48882E-5 6.787505 2 GTAGTCC 1015 0.0076361713 6.106128 1 GTCGACT 1120 0.003170059 6.036741 1 CGTGTAG 2405 0.0 5.4741044 94-95 GGTAAAT 1475 0.00202475 5.347802 1 GTTCGAA 1380 0.0054051424 5.307666 1 GTGTAGG 2395 0.0 5.1178603 94-95 >>END_MODULE