FastQCFastQC Report
Thu 11 Sep 2014
LONLib40_CTCAGA_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib40_CTCAGA_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3456037
Sequences flagged as poor quality0
Sequence length101
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG8502.093837E-47.66588833
ATGATAC11209.48882E-56.7875052
GTAGTCC10150.00763617136.1061281
GTCGACT11200.0031700596.0367411
CGTGTAG24050.05.474104494-95
GGTAAAT14750.002024755.3478021
GTTCGAA13800.00540514245.3076661
GTGTAGG23950.05.117860394-95
Produced by FastQC (version 0.11.2)