##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib40_CTCAGA_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3456037 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21831826453247 33.0 33.0 33.0 27.0 33.0 2 31.605154979532916 33.0 33.0 33.0 27.0 33.0 3 31.7900042736811 33.0 33.0 33.0 27.0 33.0 4 35.62621696469106 37.0 37.0 37.0 33.0 37.0 5 35.13920568558728 37.0 37.0 37.0 33.0 37.0 6 34.912390694891286 37.0 33.0 37.0 33.0 37.0 7 34.69544452215066 37.0 33.0 37.0 27.0 37.0 8 34.739742659005096 37.0 33.0 37.0 27.0 37.0 9 34.63911208126533 37.0 33.0 37.0 27.0 37.0 10-11 34.46669436698739 37.0 33.0 37.0 27.0 37.0 12-13 34.47069577090754 37.0 33.0 37.0 27.0 37.0 14-15 35.36337950085604 37.0 33.0 40.0 27.0 40.0 16-17 35.24987869053486 37.0 33.0 40.0 27.0 40.0 18-19 35.439905735962896 37.0 33.0 40.0 27.0 40.0 20-21 35.050064423500096 37.0 33.0 40.0 27.0 40.0 22-23 35.04871085002851 37.0 33.0 40.0 27.0 40.0 24-25 34.84212987303087 37.0 33.0 40.0 27.0 40.0 26-27 34.36800951494443 37.0 33.0 40.0 27.0 40.0 28-29 33.917175655237486 37.0 33.0 37.0 22.0 40.0 30-31 33.74495267267103 37.0 33.0 37.0 22.0 40.0 32-33 33.674428543444414 37.0 33.0 37.0 22.0 40.0 34-35 33.43732749388968 37.0 33.0 37.0 22.0 40.0 36-37 33.01705132207786 37.0 33.0 37.0 22.0 40.0 38-39 32.80476843853234 37.0 33.0 37.0 22.0 40.0 40-41 32.39732777745145 37.0 33.0 37.0 22.0 40.0 42-43 31.89970535616372 35.0 27.0 37.0 15.0 40.0 44-45 31.652126120177535 33.0 27.0 37.0 15.0 40.0 46-47 31.415586117856957 33.0 27.0 37.0 18.5 40.0 48-49 31.379102278129544 33.0 27.0 37.0 18.5 40.0 50-51 31.71369056523411 33.0 27.0 37.0 22.0 40.0 52-53 31.533500364724105 33.0 27.0 37.0 18.5 40.0 54-55 31.12853479288561 33.0 27.0 37.0 15.0 40.0 56-57 31.137759520514393 33.0 27.0 37.0 15.0 40.0 58-59 30.829673264493408 33.0 27.0 37.0 15.0 40.0 60-61 30.71434203395392 33.0 27.0 37.0 15.0 40.0 62-63 30.67534881715676 33.0 27.0 37.0 15.0 38.5 64-65 30.327842265577594 33.0 27.0 37.0 15.0 37.0 66-67 29.976524412209706 33.0 27.0 37.0 15.0 37.0 68-69 29.778158914386623 33.0 27.0 37.0 15.0 37.0 70-71 29.61527249274241 33.0 27.0 37.0 15.0 37.0 72-73 29.409530193108466 33.0 27.0 37.0 15.0 37.0 74-75 29.057314201207916 33.0 27.0 37.0 15.0 37.0 76-77 27.936908806242528 33.0 22.0 33.0 15.0 37.0 78-79 28.935566083349222 33.0 27.0 35.0 15.0 37.0 80-81 28.690937047259624 33.0 27.0 37.0 15.0 37.0 82-83 28.6842776856845 33.0 27.0 35.0 15.0 37.0 84-85 28.578160187521142 33.0 24.5 33.0 15.0 37.0 86-87 28.371024239613178 33.0 22.0 33.0 15.0 37.0 88-89 28.25654268169004 33.0 22.0 33.0 15.0 37.0 90-91 28.169160370678902 33.0 22.0 33.0 15.0 37.0 92-93 27.943873286078826 33.0 22.0 33.0 15.0 37.0 94-95 27.83511678260389 33.0 22.0 33.0 15.0 37.0 96-97 27.682843673259285 33.0 22.0 33.0 15.0 37.0 98-99 27.38752999461522 33.0 22.0 33.0 15.0 37.0 100-101 26.536782592315994 30.0 22.0 33.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.1049499020153277 2306 2 0.5468149405047988 2306 3 0.4211629700422037 2306 4 0.2529390157894724 2306 5 0.6897717927465479 2306 6 0.7602495000100191 2306 7 0.7938735905221819 2306 8 0.7934312690866463 2306 9 0.930705206456004 2306 10-11 1.1561455905975748 2306 12-13 1.369588977292615 2306 14-15 1.4900552408319143 2306 16-17 1.441525520587163 2306 18-19 1.3722269033606764 2306 20-21 1.6244786711902393 2306 22-23 1.2111012906508378 2306 24-25 1.204559610644786 2306 26-27 1.414173538780041 2306 28-29 1.7167984254164566 2306 30-31 1.5223476261800144 2306 32-33 1.137230124749955 2306 34-35 1.1090815997030745 2306 36-37 1.2526775111554826 2306 38-39 1.0136297072871372 2306 40-41 1.1392714550923841 2306 42-43 1.0797694004347633 2306 44-45 0.7821053390393651 2306 46-47 0.6823401684607759 2306 48-49 0.8485189431201938 2306 50-51 0.8295071257688704 2306 52-53 0.9204022040196911 2306 54-55 1.4608831792279382 2306 56-57 0.9451280754865969 2306 58-59 0.9117601547390315 2306 60-61 0.7958051560420394 2306 62-63 0.5260955194266472 2306 64-65 0.5888034894945307 2306 66-67 0.7345166162338401 2306 68-69 0.4968698995515446 2306 70-71 0.49803422644458806 2306 72-73 0.19390129203629058 2306 74-75 0.47463394410283044 2306 76-77 0.4497313749467864 2306 78-79 0.5154821839408008 2306 80-81 0.9523561675970171 2306 82-83 0.8530397536715739 2306 84-85 0.5189399391348317 2306 86-87 0.32141234860352697 2306 88-89 0.16207828171804906 2306 90-91 -0.24910366713062615 2306 92-93 -0.447330385216933 2306 94-95 -0.43803861217724105 2306 96-97 -0.2041056951989333 2306 98-99 -0.18813470516250064 2306 100-101 -0.2532265409224621 2307 1 0.7225607532580618 2307 2 0.4558802923404386 2307 3 0.3358165966722524 2307 4 0.27061906501234745 2307 5 0.5312390120253738 2307 6 0.6348600184500341 2307 7 0.8531313189891705 2307 8 0.7103826573320831 2307 9 1.0280585398795665 2307 10-11 1.169143063864908 2307 12-13 1.290788528095696 2307 14-15 1.3411284168283402 2307 16-17 1.4072562620154088 2307 18-19 1.2088380311871205 2307 20-21 1.4607160639880306 2307 22-23 1.2611978346732755 2307 24-25 1.1906211628380703 2307 26-27 1.2842643949644952 2307 28-29 1.4849621664383292 2307 30-31 1.6029831752715467 2307 32-33 1.2814250770453413 2307 34-35 1.1899122496192405 2307 36-37 1.1840468052285118 2307 38-39 0.9829579975660749 2307 40-41 0.9762452129556678 2307 42-43 1.110343004493913 2307 44-45 0.8196614322616078 2307 46-47 0.9327971041701595 2307 48-49 1.0511803758654175 2307 50-51 0.9754439133793085 2307 52-53 1.1297991786728936 2307 54-55 1.3675468683686738 2307 56-57 1.0462285801737714 2307 58-59 1.0494785543570195 2307 60-61 0.8570015211005959 2307 62-63 0.6415180799809512 2307 64-65 0.7502148923083283 2307 66-67 0.840887827653102 2307 68-69 0.6859539875905334 2307 70-71 0.640043716354139 2307 72-73 0.503924362656754 2307 74-75 0.7046594647621056 2307 76-77 0.8427824610323356 2307 78-79 0.7762927546436806 2307 80-81 1.4324043146770826 2307 82-83 1.0818300869150264 2307 84-85 0.9045868413453171 2307 86-87 0.9208074909330861 2307 88-89 0.6307278834826313 2307 90-91 0.41488995860408195 2307 92-93 0.43603192520437517 2307 94-95 0.39089525308520123 2307 96-97 0.26548751854057784 2307 98-99 0.34883532211760837 2307 100-101 0.33263189403022153 1101 1 1.084706953301236 1101 2 0.534635177928557 1101 3 0.3901417928641848 1101 4 0.293017006873896 1101 5 0.4765504549091659 1101 6 0.5298301281719588 1101 7 0.6752481816564071 1101 8 0.5581335821624762 1101 9 0.8061661236830986 1101 10-11 0.9755768132939053 1101 12-13 1.2122159059016795 1101 14-15 1.191394551766848 1101 16-17 1.275887315189756 1101 18-19 0.9219773883156108 1101 20-21 1.3315428982187854 1101 22-23 0.9492027199964284 1101 24-25 0.9789804531307738 1101 26-27 1.0569671219202235 1101 28-29 1.3296961003917858 1101 30-31 1.3144302809083541 1101 32-33 0.7172870691393669 1101 34-35 0.8066377158676588 1101 36-37 1.0499286276080824 1101 38-39 0.7053551621850005 1101 40-41 0.8405196975996532 1101 42-43 0.9563626743848488 1101 44-45 0.7351694009415546 1101 46-47 0.6022311726219165 1101 48-49 0.7577950991723412 1101 50-51 0.7110050949152722 1101 52-53 0.8961120174137811 1101 54-55 1.2757981996616294 1101 56-57 1.0324563532429494 1101 58-59 1.123269050022305 1101 60-61 0.8208016934364828 1101 62-63 0.45622351032782404 1101 64-65 0.4298795580463093 1101 66-67 0.6082743740779826 1101 68-69 0.46967995798677364 1101 70-71 0.7211515803183097 1101 72-73 0.47532513756764416 1101 74-75 0.5924943989208238 1101 76-77 0.5656729274097003 1101 78-79 0.6212434686293484 1101 80-81 1.1885432065198884 1101 82-83 1.100186967755608 1101 84-85 0.8472297100206383 1101 86-87 0.6566489567561362 1101 88-89 0.48623584862965785 1101 90-91 0.3695379224580577 1101 92-93 0.411395913222119 1101 94-95 0.3529730204487116 1101 96-97 0.3144359092773996 1101 98-99 0.4083420945505054 1101 100-101 0.40534431885407685 2201 1 1.432950745229423 2201 2 0.7210667059843061 2201 3 0.3211970551889962 2201 4 0.32380502497987607 2201 5 0.37523311423293393 2201 6 0.45056061349835375 2201 7 0.6416460737404961 2201 8 0.637142952793269 2201 9 0.6068425622561691 2201 10-11 0.8664417695477482 2201 12-13 1.0601160332955644 2201 14-15 1.148657702618543 2201 16-17 1.0374720887110485 2201 18-19 1.003484739667023 2201 20-21 1.1775403563913613 2201 22-23 0.9573272521010594 2201 24-25 0.8755383892323891 2201 26-27 1.1473700595780087 2201 28-29 1.4503940832833848 2201 30-31 1.46634738181573 2201 32-33 0.9873773463647666 2201 34-35 1.0154669537874383 2201 36-37 1.083000313227231 2201 38-39 0.7137013123147469 2201 40-41 1.0172624660525926 2201 42-43 1.0231206468237062 2201 44-45 0.9468414229916071 2201 46-47 0.95191576643224 2201 48-49 1.0608316255740569 2201 50-51 0.9901018119469214 2201 52-53 1.217558401564613 2201 54-55 1.499570345226271 2201 56-57 1.089336406001351 2201 58-59 1.2022580810511379 2201 60-61 1.0597469774159407 2201 62-63 0.9193770223613775 2201 64-65 0.8593378502066287 2201 66-67 0.8399211308901933 2201 68-69 0.8708325443282057 2201 70-71 1.0383334101315391 2201 72-73 0.8951309005972234 2201 74-75 1.0126311742913643 2201 76-77 0.9144450151408279 2201 78-79 0.8590722098927053 2201 80-81 1.357264795806202 2201 82-83 1.2189692110517676 2201 84-85 1.105129109601993 2201 86-87 1.0857877854833404 2201 88-89 1.0364548774928544 2201 90-91 0.8196925870690563 2201 92-93 0.8706004995443308 2201 94-95 0.8430902249581571 2201 96-97 0.612258557557638 2201 98-99 0.7378723404995355 2201 100-101 0.778579751615144 2304 1 -1.8377034831712855 2304 2 -0.3362054115695017 2304 3 -0.19668356184654812 2304 4 -0.07044410617066177 2304 5 0.359899902843388 2304 6 0.4620404013161732 2304 7 0.5259214228045721 2304 8 0.6122371543663405 2304 9 0.825519472200682 2304 10-11 0.9080185026737695 2304 12-13 1.1731542626718223 2304 14-15 1.1985766100022346 2304 16-17 1.1640192504694724 2304 18-19 0.8597337488737793 2304 20-21 1.1997402222531974 2304 22-23 0.8540628170056337 2304 24-25 0.969827761831624 2304 26-27 0.9707515607059918 2304 28-29 1.473192693124247 2304 30-31 1.5227378458118324 2304 32-33 0.951938043883203 2304 34-35 0.8352657465439819 2304 36-37 1.1495304028559161 2304 38-39 0.9397375296185686 2304 40-41 0.9982745994590942 2304 42-43 0.8322444807362643 2304 44-45 0.960732039417433 2304 46-47 0.895981372187979 2304 48-49 0.7939930181720634 2304 50-51 0.9563380170563676 2304 52-53 1.0535696203927394 2304 54-55 1.447343042013049 2304 56-57 1.0723362230856779 2304 58-59 0.887315089770123 2304 60-61 0.5915340635692239 2304 62-63 0.362378058883543 2304 64-65 0.2982281617729967 2304 66-67 0.36741701528748294 2304 68-69 0.5180211303900322 2304 70-71 0.5594869983273831 2304 72-73 0.38718016588592263 2304 74-75 0.4741737631578964 2304 76-77 0.6567814580406512 2304 78-79 0.6405026104780376 2304 80-81 1.329259186392143 2304 82-83 0.9862321828217837 2304 84-85 0.6655437184673083 2304 86-87 0.6035867001836621 2304 88-89 0.3586157213438419 2304 90-91 0.1626167990073526 2304 92-93 0.23327594616344172 2304 94-95 0.1122945417681116 2304 96-97 0.03809356761799876 2304 98-99 0.4023501162449179 2304 100-101 0.5722276999109788 1102 1 1.4254789521336413 1102 2 0.14348959247608306 1102 3 0.3003909089132435 1102 4 0.07916463777810634 1102 5 0.20511518738285162 1102 6 0.15668075568197537 1102 7 -5.107733271673773 1102 8 -1.23541956061878 1102 9 0.18596672645997359 1102 10-11 0.644498706735817 1102 12-13 0.9786915483358243 1102 14-15 0.9208312578535001 1102 16-17 0.7404744039045639 1102 18-19 0.8984713435564586 1102 20-21 1.1817586262357054 1102 22-23 0.7343929550126447 1102 24-25 1.0748516924944127 1102 26-27 1.107544623327982 1102 28-29 0.9697585630164127 1102 30-31 1.3181628150699538 1102 32-33 1.1000337848194945 1102 34-35 1.2992653442711415 1102 36-37 1.326668871567776 1102 38-39 1.0727168628513368 1102 40-41 1.2751518651008809 1102 42-43 1.3614770081525336 1102 44-45 1.2435872623360105 1102 46-47 0.9489468087225852 1102 48-49 1.072877076739335 1102 50-51 0.9426032337122905 1102 52-53 1.1925149341426966 1102 54-55 1.586317154841442 1102 56-57 1.309113286219656 1102 58-59 1.2263687429103634 1102 60-61 1.1779035054613693 1102 62-63 0.9233972719830064 1102 64-65 0.6603899211681252 1102 66-67 0.7888859511386777 1102 68-69 0.8942194691860479 1102 70-71 0.8019026467409205 1102 72-73 0.7308564825295036 1102 74-75 0.81439501322075 1102 76-77 0.7261537752693599 1102 78-79 0.8796772197476841 1102 80-81 1.5340747580081349 1102 82-83 1.2773096797704504 1102 84-85 1.0328649013323137 1102 86-87 0.8760609981489864 1102 88-89 0.7276177647070234 1102 90-91 0.41727059294309043 1102 92-93 0.5521947464761183 1102 94-95 0.478226420519416 1102 96-97 0.42610382557545634 1102 98-99 0.3776391355677262 1102 100-101 0.5078327776913483 2202 1 1.4503263164864215 2202 2 0.8941196197992092 2202 3 0.3623008532467722 2202 4 -0.2685326653709623 2202 5 0.022016670552893913 2202 6 0.6221835064621146 2202 7 0.7062722121173621 2202 8 -4.283678554050788 2202 9 -0.5466976999257298 2202 10-11 0.637695877671625 2202 12-13 0.03415634246005794 2202 14-15 0.16115648050042353 2202 16-17 0.2305317132383209 2202 18-19 0.7074512119741101 2202 20-21 1.221594204912627 2202 22-23 -2.968116467124865 2202 24-25 -3.2210440549505712 2202 26-27 -1.669694893954869 2202 28-29 0.572732880673513 2202 30-31 1.3122694637495727 2202 32-33 1.0827005428703202 2202 34-35 -2.1418365952435536 2202 36-37 -0.5133348921818168 2202 38-39 0.2856776209131766 2202 40-41 1.1675940642635254 2202 42-43 1.3004442975577533 2202 44-45 1.458487095856551 2202 46-47 0.7369972517517915 2202 48-49 1.0284267976654 2202 50-51 -3.945053304800073 2202 52-53 -0.351846570525435 2202 54-55 1.5109060851336658 2202 56-57 1.4264025160288796 2202 58-59 1.5245551837657452 2202 60-61 1.3783856766942364 2202 62-63 1.1171939104549224 2202 64-65 1.06910444752166 2202 66-67 1.2992090953949322 2202 68-69 1.147877951579808 2202 70-71 1.32548867296207 2202 72-73 1.1626217218720072 2202 74-75 0.5166587049508387 2202 76-77 1.124009986743939 2202 78-79 1.3234244796846504 2202 80-81 1.9956136638299995 2202 82-83 1.8880989830663495 2202 84-85 1.6159402041899433 2202 86-87 1.58476459205923 2202 88-89 1.4402004364963368 2202 90-91 1.3138390122261612 2202 92-93 1.5304029900403435 2202 94-95 1.4227886081101637 2202 96-97 1.3890494599011731 2202 98-99 1.4929159315980272 2202 100-101 1.3057499575739087 2305 1 1.3282037290273507 2305 2 0.6926017553123636 2305 3 0.11146402964665114 2305 4 -0.2636741901319368 2305 5 0.2263362965391167 2305 6 0.2584827385686097 2305 7 0.6005182970858272 2305 8 -0.0437008686837288 2305 9 0.5266083955515768 2305 10-11 0.39519909343142956 2305 12-13 0.8618584534485976 2305 14-15 0.7738413487703966 2305 16-17 0.8470199951219826 2305 18-19 0.722261012947591 2305 20-21 0.9104989707815463 2305 22-23 0.6107209440793397 2305 24-25 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-0.6809823048523214 1208 42-43 0.028429207803057466 1208 44-45 0.18641869501488983 1208 46-47 0.11885782180377902 1208 48-49 -1.3746558948574226 1208 50-51 -0.22604761185057143 1208 52-53 -1.4617504213098584 1208 54-55 -0.8345878912756604 1208 56-57 -5.863850305945256 1208 58-59 -1.8601850282156285 1208 60-61 -1.2045340506753028 1208 62-63 0.3607985944101202 1208 64-65 -0.03599868835078013 1208 66-67 0.8862117587006608 1208 68-69 0.6260314616511096 1208 70-71 -4.233814242882676 1208 72-73 -0.7130875545007243 1208 74-75 -5.284518954761779 1208 76-77 -1.0849865262050358 1208 78-79 -3.6503509627739525 1208 80-81 1.0165968701817576 1208 82-83 0.6912690158269008 1208 84-85 -2.609186051267418 1208 86-87 -0.7668691538400765 1208 88-89 -4.760598536482906 1208 90-91 0.07807293190649744 1208 92-93 -1.8075714060218715 1208 94-95 -4.656163706293707 1208 96-97 -3.5434147621757646 1208 98-99 -6.2937524927417385 1208 100-101 -3.6591958946642507 1209 1 1.7256507655762228 1209 2 1.3472266619749327 1209 3 0.6209586710765791 1209 4 0.40586894407497454 1209 5 0.46328301161975105 1209 6 1.1572888731938562 1209 7 0.8177579258770322 1209 8 0.5511407975396452 1209 9 -0.042551932050493235 1209 10-11 -2.9540743358235595 1209 12-13 -0.42102800948797636 1209 14-15 -1.3109267705123813 1209 16-17 -0.35262228527317774 1209 18-19 -16.672550246781004 1209 20-21 -13.96828189295993 1209 22-23 -3.390895919638492 1209 24-25 -2.2139616572386984 1209 26-27 -1.4536231656912122 1209 28-29 -3.0980760132006324 1209 30-31 -1.007458499665411 1209 32-33 -0.6991266966197429 1209 34-35 -3.008561612114839 1209 36-37 -4.505169404203443 1209 38-39 -1.7999343091543878 1209 40-41 -10.079700334470896 1209 42-43 -4.863135969026203 1209 44-45 -2.604435527098449 1209 46-47 -2.486638405038537 1209 48-49 -2.919129539669594 1209 50-51 -2.483232325210608 1209 52-53 -0.7914640226184311 1209 54-55 -2.394994296054623 1209 56-57 -0.6513526722886134 1209 58-59 -1.700903208785256 1209 60-61 -1.399254723174618 1209 62-63 -0.47592990032329396 1209 64-65 0.07051150888780455 1209 66-67 0.16447394088373457 1209 68-69 -0.8032863261983252 1209 70-71 -0.952975994360326 1209 72-73 -0.4504562301774371 1209 74-75 0.7231090226549526 1209 76-77 -15.616205554946362 1209 78-79 -15.350623417052486 1209 80-81 -20.401876569238 1209 82-83 -9.64160898973849 1209 84-85 -3.247691685329894 1209 86-87 -2.125132919771911 1209 88-89 -2.0395703123478093 1209 90-91 -5.942644481534828 1209 92-93 -2.9962274343511766 1209 94-95 -0.43276666419196985 1209 96-97 0.5468255098330843 1209 98-99 1.9349197937584144 1209 100-101 1.191919967693135 1212 1 -28.603937249405053 1212 2 -16.23982303106341 1212 3 -5.727822491645274 1212 4 -1.1984304497240146 1212 5 -0.40181473990218564 1212 6 -2.612090896938838 1212 7 -0.25204528402811377 1212 8 -1.1656822458211025 1212 9 -0.02114183899671218 1212 10-11 0.7450147538452825 1212 12-13 0.30488965786081934 1212 14-15 0.05800156648904675 1212 16-17 0.13812220936625152 1212 18-19 -0.7512083674291645 1212 20-21 -0.5030580104419471 1212 22-23 -0.3457461712569554 1212 24-25 0.5609363439711359 1212 26-27 -0.32229570131034535 1212 28-29 0.38130221247088514 1212 30-31 -0.23900379742465816 1212 32-33 0.5516214017624392 1212 34-35 -0.9401098057387856 1212 36-37 -0.8585405124830388 1212 38-39 -0.2649458282606645 1212 40-41 -0.2923839001169313 1212 42-43 0.29783871468102063 1212 44-45 1.0965181776777975 1212 46-47 -1.7496456640060636 1212 48-49 -0.7869542304555956 1212 50-51 0.21993510725139487 1212 52-53 -0.8852771685571419 1212 54-55 -0.10122169222178812 1212 56-57 -1.031603840287378 1212 58-59 -0.12489158671490586 1212 60-61 0.5577352506982471 1212 62-63 -3.9162734785824327 1212 64-65 -3.4534099423908806 1212 66-67 -0.9610392706945738 1212 68-69 0.8434616177377379 1212 70-71 -0.015417272659359327 1212 72-73 -0.5981324596328861 1212 74-75 0.4313313478412546 1212 76-77 1.6303580189367999 1212 78-79 -0.5692032850010555 1212 80-81 1.0414513584812184 1212 82-83 1.203624738719494 1212 84-85 -3.0154787298747294 1212 86-87 -1.2232850363272476 1212 88-89 -0.36892344052521864 1212 90-91 0.7948823856543825 1212 92-93 -1.9980035647036978 1212 94-95 -1.7897769091177729 1212 96-97 0.4732850256445076 1212 98-99 0.12019721759717328 1212 100-101 0.6323890334228288 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 4.0 10 7.0 11 22.0 12 145.0 13 671.0 14 1992.0 15 4413.0 16 8440.0 17 13848.0 18 20892.0 19 29155.0 20 38985.0 21 50881.0 22 63460.0 23 77816.0 24 92896.0 25 109586.0 26 128705.0 27 148860.0 28 170361.0 29 194806.0 30 219426.0 31 246255.0 32 274282.0 33 297975.0 34 317305.0 35 326954.0 36 307927.0 37 236056.0 38 73761.0 39 148.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.431861938437294 22.207599306335744 12.254210700516985 20.10632805470997 2 25.93991576262143 23.449716257304797 27.130587613008068 23.479780367065707 3 22.08058897619842 21.71815009761197 30.006906766750646 26.194354159438966 4 27.407766414402634 21.480540561655193 25.511344647840662 25.60034837610152 5 24.913564293437833 24.619933177798732 25.010496126054203 25.45600640270923 6 24.056571153607443 25.078464148387305 24.800168516714375 26.064796181290884 7 26.78018204087514 24.740331194370892 25.055316248061 23.424170516692964 8 23.703883957260874 24.089441172070785 25.843965212177995 26.362709658490346 9 25.30236221429342 24.03822644259885 24.73995504099059 25.919456302117133 10-11 25.85384647212978 24.36854698025513 23.98339485370093 25.794211693914153 12-13 25.663870099273044 24.1542426185159 24.10635198772933 26.07553529448172 14-15 25.21298603183155 24.577879892182853 24.386562468307265 25.822571607678334 16-17 25.676543725705447 24.431759983009368 24.126018295022426 25.765677996262752 18-19 25.907872581573795 24.457805410611925 23.8341112611846 25.80021074662968 20-21 25.321169883308542 24.606825679238966 24.387340181832545 25.68466425561995 22-23 25.612399404288784 24.311747819829474 24.16413655293621 25.91171622294553 24-25 25.639786263862334 23.969115492687145 24.3746811738416 26.01641706960892 26-27 25.428720815199608 24.331568209483866 24.37269913487616 25.86701184044037 28-29 25.94475985066132 24.24256453272925 24.10231719162729 25.71035842498214 30-31 25.81339551630958 24.0729482930883 24.080225414253377 26.033430776348748 32-33 25.445951533504996 24.33036741215444 24.30353031521364 25.920150739126925 34-35 25.94876733090531 24.083683131864618 24.096906369926018 25.870643167304053 36-37 25.84111512695032 24.12048829338343 24.061924105557896 25.97647247410835 38-39 25.634896269918407 24.273047423971445 24.34973931124001 25.742316994870134 40-41 25.91623631264427 24.19363869359493 24.084754725334186 25.805370268426614 42-43 25.69563057339953 23.96912996012485 24.293576718073332 26.04166274840229 44-45 25.516335617934644 24.158277240666116 24.459402489035853 25.865984652363387 46-47 25.90192425267438 24.11041209178819 24.229260651402402 25.758403004135026 48-49 25.90423333325135 23.93285601377648 24.24662593888286 25.91628471408931 50-51 25.512451852297662 24.235365876241886 24.347184864029035 25.904997407431413 52-53 25.884344854346008 24.138123102412507 24.267245133365336 25.71028690987615 54-55 25.630037941673265 23.953269168863955 24.41400912191611 26.00268376754667 56-57 25.449106431232742 24.238892836128688 24.519419334742764 25.792581397895802 58-59 25.81008968252704 24.056986707639016 24.400033596554614 25.73289001327933 60-61 25.7969095120749 23.972541599132985 24.23048494187558 26.000063946916534 62-63 25.572733282050443 24.190930914655944 24.477071499121745 25.759264304171868 64-65 25.884839178151875 24.17618571010661 24.149825504699553 25.789149607041967 66-67 25.78508778465866 24.121325112927376 24.184189140134407 25.909397962279556 68-69 25.565395866085737 24.300498314019723 24.352280030429537 25.781825789465003 70-71 25.976012406567705 24.20438139113227 24.035323242507804 25.784282959792225 72-73 25.74009842024536 24.0537522392907 24.228597099031283 25.97755224143266 74-75 25.496561307607706 24.20465448294997 24.465626608822745 25.833157600619582 76-77 25.80041154976554 24.247732035052877 24.17510731056532 25.776749104616265 78-79 25.88984829245279 24.0965305656832 24.05068308977555 25.962938052088465 80-81 25.499968278973533 24.208676265632747 24.41747014529968 25.873885310094035 82-83 25.855689016079886 24.124518572747238 24.113710874541 25.906081536631874 84-85 25.739375211416444 23.981895084200534 24.210506974102227 26.068222730280795 86-87 25.51566591628534 24.247391738841255 24.355456488565522 25.881485856307883 88-89 25.851276455307787 24.142125904390355 24.04071612176813 25.965881518533728 90-91 25.855265709319347 24.005192689831855 23.97674316299698 26.16279843785182 92-93 25.501192536992956 24.172805873729402 24.296356873278548 26.029644715999094 94-95 25.908574281135717 24.127737001841247 23.939922515672308 26.02376620135073 96-97 25.815055009322315 24.00739708196952 23.96513341630767 26.212414492400498 98-99 25.574963982683908 24.156467005505075 24.158912290799694 26.109656721011326 100-101 25.9337741943372 23.951201916085736 23.80517750025936 26.309846389317705 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 4.0 2 6.5 3 10.5 4 19.5 5 37.5 6 61.5 7 93.0 8 143.5 9 238.0 10 348.0 11 531.0 12 776.5 13 1050.0 14 1452.0 15 2037.0 16 2720.5 17 3624.5 18 4772.5 19 6177.5 20 7985.5 21 10017.0 22 12224.0 23 14948.5 24 18133.0 25 21590.5 26 25412.0 27 29409.0 28 33575.5 29 37990.0 30 42540.5 31 46881.5 32 51225.5 33 55374.0 34 59219.5 35 62876.5 36 66121.5 37 68906.0 38 70791.5 39 71924.5 40 72429.0 41 72503.5 42 71909.5 43 71154.5 44 70105.0 45 68824.5 46 68207.5 47 68135.5 48 67842.5 49 66150.5 50 64325.0 51 63471.5 52 62448.5 53 61196.0 54 60451.5 55 60105.0 56 60318.5 57 61220.0 58 62599.5 59 64723.5 60 67721.0 61 70761.5 62 74637.0 63 79286.5 64 83579.5 65 87506.0 66 90347.5 67 91778.0 68 91786.0 69 89853.5 70 85409.0 71 79027.0 72 70583.5 73 60730.0 74 50636.5 75 40102.0 76 30205.0 77 21688.5 78 14778.5 79 9574.5 80 5944.0 81 3674.5 82 2297.5 83 1524.0 84 1028.0 85 672.0 86 464.0 87 341.5 88 236.5 89 160.0 90 118.5 91 82.5 92 50.0 93 36.5 94 20.0 95 9.5 96 8.5 97 4.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6894891460942114 2 0.0025752039113007183 3 1.7360925244723943E-4 4 5.7869750815746473E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.18838050634295872 14-15 1.4467437703936618E-5 16-17 4.3402313111809854E-4 18-19 0.11644840607898585 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0018662994638078238 42-43 0.0 44-45 0.0 46-47 0.02313343288859465 48-49 4.340231311180986E-5 50-51 1.446743770393662E-4 52-53 1.1573950163149295E-4 54-55 0.002155648217886556 56-57 0.0010271880769794999 58-59 0.09554295859679743 60-61 0.0013165368310582323 62-63 0.0012586670802424858 64-65 0.002025441278551127 66-67 0.004788721880003021 68-69 0.005324017075048676 70-71 0.003631326863688091 72-73 0.03645794301392028 74-75 0.04205684140534375 76-77 0.15687042702378476 78-79 3.6168594259841544E-4 80-81 0.11770707315922833 82-83 0.004832124193114831 84-85 0.0052082775734171825 86-87 0.006900967784777766 88-89 0.007407328104415549 90-91 0.023003225949259222 92-93 0.010966317779583958 94-95 0.014452970266232682 96-97 0.010127206392755633 98-99 0.011790961728708345 100-101 0.013035161371246893 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3456037.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.56416339877374 #Duplication Level Percentage of deduplicated Percentage of total 1 98.18459906329988 95.79298266254877 2 1.576979412453804 3.0771335414629024 3 0.08557215383525589 0.2504632679750372 4 0.05461541878482394 0.21314030569660078 5 0.046316022933120156 0.22593920147141433 6 0.01717393137878178 0.1005336148343276 7 0.01473753896859399 0.10064989620193897 8 0.009682692087069976 0.0755747002338327 9 0.004308392363233644 0.037831022686130054 >10 0.0057239757323804905 0.06332968314623347 >50 2.6110792649999636E-4 0.013246883730995538 >100 0.0 0.0 >500 0.0 0.0 >1k 3.029023654074573E-5 0.049175220011820425 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.446743770393662E-4 0.0 0.0 2 1.446743770393662E-4 0.0 0.0 3 1.7360925244723943E-4 0.0 2.8934875407873237E-5 4 3.182836294866056E-4 0.0 2.8934875407873237E-5 5 4.050882557102253E-4 2.8934875407873237E-5 2.8934875407873237E-5 6 4.050882557102253E-4 2.8934875407873237E-5 5.7869750815746473E-5 7 5.208277573417183E-4 2.8934875407873237E-5 5.7869750815746473E-5 8 6.365672589732112E-4 2.8934875407873237E-5 5.7869750815746473E-5 9 6.655021343810845E-4 2.8934875407873237E-5 5.7869750815746473E-5 10-11 7.23371885196831E-4 2.8934875407873237E-5 5.7869750815746473E-5 12-13 8.246439491243873E-4 5.7869750815746473E-5 5.7869750815746473E-5 14-15 9.403834507558803E-4 7.23371885196831E-5 5.7869750815746473E-5 16-17 0.0010127206392755634 8.680462622361972E-5 5.7869750815746473E-5 18-19 0.0010850578277952464 1.0127206392755633E-4 5.7869750815746473E-5 20-21 0.001099525265499183 1.1573950163149295E-4 5.7869750815746473E-5 22-23 0.0011139927032031197 1.1573950163149295E-4 7.23371885196831E-5 24-25 0.0011863298917228027 1.3020693933542957E-4 8.680462622361972E-5 26-27 0.0013599391441700421 1.446743770393662E-4 1.0127206392755633E-4 28-29 0.0018662994638078238 1.446743770393662E-4 1.446743770393662E-4 30-31 0.002618606224412528 1.446743770393662E-4 1.446743770393662E-4 32-33 0.0037181314899117114 1.446743770393662E-4 1.446743770393662E-4 34-35 0.005584430953719535 1.446743770393662E-4 1.446743770393662E-4 36-37 0.00813069998961238 1.446743770393662E-4 1.446743770393662E-4 38-39 0.011545015287741421 1.446743770393662E-4 1.446743770393662E-4 40-41 0.015928648912034216 1.7360925244723943E-4 1.446743770393662E-4 42-43 0.02138287292641832 2.1701156555904927E-4 1.7360925244723943E-4 44-45 0.028804668468537808 2.314790032629859E-4 1.7360925244723943E-4 46-47 0.037731077531866705 2.314790032629859E-4 1.7360925244723943E-4 48-49 0.04862505812293098 2.314790032629859E-4 1.7360925244723943E-4 50-51 0.06210871006299991 2.314790032629859E-4 2.0254412785511265E-4 52-53 0.07876073086023094 2.459464409669225E-4 2.0254412785511265E-4 54-55 0.0989283390195186 2.6041387867085914E-4 2.0254412785511265E-4 56-57 0.12214857653433688 2.6041387867085914E-4 2.0254412785511265E-4 58-59 0.14979584998655976 2.6041387867085914E-4 2.0254412785511265E-4 60-61 0.18179782218766755 2.6041387867085914E-4 2.0254412785511265E-4 62-63 0.21828470007699569 2.6041387867085914E-4 2.0254412785511265E-4 64-65 0.26034154148233946 2.893487540787324E-4 2.0254412785511265E-4 66-67 0.3091112739823098 2.893487540787324E-4 2.0254412785511265E-4 68-69 0.3635667094999272 3.3275106719054225E-4 2.0254412785511265E-4 70-71 0.4239103921630468 3.4721850489447887E-4 2.0254412785511265E-4 72-73 0.4908656938568655 3.4721850489447887E-4 2.0254412785511265E-4 74-75 0.5645772889584226 3.4721850489447887E-4 2.0254412785511265E-4 76-77 0.6459710934807701 3.4721850489447887E-4 2.0254412785511265E-4 78-79 0.7430331330364808 3.761533803023521E-4 2.459464409669225E-4 80-81 0.8472276193802324 3.761533803023521E-4 2.7488131637479576E-4 82-83 0.9606233961036875 3.761533803023521E-4 2.893487540787324E-4 84-85 1.0820630681905317 3.761533803023521E-4 2.893487540787324E-4 86-87 1.221456830467961 3.761533803023521E-4 3.03816191782669E-4 88-89 1.369820404121831 3.761533803023521E-4 3.182836294866056E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTCAGA 2480 0.0 10.905702 32-33 CTCGTAT 1100 0.0 10.142632 42-43 CGTATGC 1070 0.0 9.761522 44-45 TCGTATG 1175 0.0 9.697256 42-43 GAATCTC 2380 0.0 9.670003 38-39 AGTCACC 2830 0.0 9.30544 28-29 ACCTCAG 2990 0.0 8.966184 32-33 CAGTCAC 3060 0.0 8.761075 26-27 TCTCGTA 1335 0.0 8.710709 40-41 CACCTCA 3370 0.0 8.659157 30-31 CTCAGAA 3250 0.0 8.614508 34-35 CAGAATC 3105 0.0 8.176247 36-37 AATCTCG 2225 0.0 8.104298 38-39 CCAGTCA 3355 0.0 7.9907274 26-27 GTCACCT 3580 0.0 7.8861375 28-29 GAGCACA 6985 0.0 7.7052274 9 AGAATCT 3090 0.0 7.6016617 36-37 GTATGCC 1445 0.0 7.556813 44-45 TCAGAAT 3575 0.0 7.4995327 34-35 CTCCAGT 3690 0.0 7.458165 24-25 >>END_MODULE