FastQCFastQC Report
Thu 11 Sep 2014
LONLib40_CTCAGA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib40_CTCAGA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3456037
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCAGA24800.010.90570232-33
CTCGTAT11000.010.14263242-43
CGTATGC10700.09.76152244-45
TCGTATG11750.09.69725642-43
GAATCTC23800.09.67000338-39
AGTCACC28300.09.3054428-29
ACCTCAG29900.08.96618432-33
CAGTCAC30600.08.76107526-27
TCTCGTA13350.08.71070940-41
CACCTCA33700.08.65915730-31
CTCAGAA32500.08.61450834-35
CAGAATC31050.08.17624736-37
AATCTCG22250.08.10429838-39
CCAGTCA33550.07.990727426-27
GTCACCT35800.07.886137528-29
GAGCACA69850.07.70522749
AGAATCT30900.07.601661736-37
GTATGCC14450.07.55681344-45
TCAGAAT35750.07.499532734-35
CTCCAGT36900.07.45816524-25
Produced by FastQC (version 0.11.2)