##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib39_CTATAC_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1832813 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.81670470473529 27.0 22.0 33.0 2.0 33.0 2 24.827933346173342 27.0 15.0 33.0 2.0 33.0 3 25.71142391504207 33.0 22.0 33.0 6.0 33.0 4 27.87762199416962 33.0 22.0 37.0 2.0 37.0 5 27.95683574920082 33.0 15.0 37.0 6.0 37.0 6 29.007096741456984 33.0 22.0 37.0 15.0 37.0 7 28.678242679422286 33.0 27.0 37.0 6.0 37.0 8 28.45260864037957 33.0 27.0 37.0 2.0 37.0 9 29.210037248753693 33.0 27.0 37.0 15.0 37.0 10-11 28.832228383364807 33.0 24.5 37.0 10.5 37.0 12-13 29.12522308604315 33.0 27.0 37.0 10.5 37.0 14-15 29.46283117808527 33.0 27.0 37.0 6.0 40.0 16-17 30.313010110687777 33.0 27.0 37.0 15.0 40.0 18-19 30.626357680789038 33.0 27.0 37.0 15.0 40.0 20-21 30.627801636064344 33.0 27.0 37.0 15.0 40.0 22-23 30.65522451008368 33.0 27.0 37.0 15.0 40.0 24-25 30.253675906925587 33.0 27.0 37.0 10.5 40.0 26-27 29.723489248493983 33.0 27.0 37.0 10.5 40.0 28-29 29.917943347193628 33.0 27.0 37.0 15.0 40.0 30-31 29.824290312214067 33.0 27.0 37.0 15.0 40.0 32-33 29.650602107252624 33.0 27.0 37.0 15.0 40.0 34-35 29.680654272967292 33.0 27.0 37.0 15.0 40.0 36-37 29.907308328782044 33.0 27.0 37.0 15.0 40.0 38-39 29.936068218634418 33.0 27.0 37.0 15.0 40.0 40-41 30.014398086438717 33.0 27.0 37.0 15.0 40.0 42-43 29.913134618752707 33.0 27.0 37.0 15.0 40.0 44-45 29.66453260643612 33.0 27.0 37.0 15.0 40.0 46-47 29.284320058838517 33.0 22.0 37.0 10.5 40.0 48-49 29.118858825204754 33.0 22.0 37.0 10.5 40.0 50-51 28.355397686507025 33.0 22.0 37.0 10.5 38.5 52-53 28.085822448880492 33.0 22.0 37.0 6.0 37.0 54-55 29.012523645347343 33.0 22.0 37.0 15.0 40.0 56-57 28.927988834649252 33.0 22.0 37.0 10.5 40.0 58-59 29.123066837697024 33.0 22.0 37.0 15.0 40.0 60-61 28.883743186020617 33.0 22.0 37.0 10.5 40.0 62-63 28.756934286258335 33.0 22.0 37.0 15.0 40.0 64-65 28.719344526692034 33.0 22.0 37.0 10.5 40.0 66-67 28.6018360847506 33.0 22.0 37.0 15.0 40.0 68-69 28.309883768829664 33.0 22.0 37.0 6.0 40.0 70-71 28.223869811050008 33.0 22.0 37.0 10.5 37.0 72-73 27.991703736278605 33.0 22.0 37.0 15.0 37.0 74-75 27.936882267858206 33.0 22.0 37.0 15.0 37.0 76-77 27.552620207298837 33.0 22.0 37.0 6.0 37.0 78-79 26.996338415321148 27.0 22.0 33.0 6.0 37.0 80-81 26.878780595729076 27.0 22.0 33.0 6.0 37.0 82-83 26.467810409463485 27.0 22.0 33.0 6.0 37.0 84-85 26.572369085116705 27.0 22.0 33.0 6.0 37.0 86-87 26.267174283464815 27.0 22.0 33.0 6.0 37.0 88-89 25.91198665657653 27.0 22.0 33.0 6.0 37.0 90-91 25.79144326235137 27.0 22.0 33.0 6.0 37.0 92-93 25.67609679765475 27.0 22.0 33.0 6.0 37.0 94-95 25.478397959857332 27.0 22.0 33.0 6.0 37.0 96-97 25.36138220320349 27.0 22.0 33.0 6.0 37.0 98-99 25.139923440089085 27.0 15.0 33.0 6.0 37.0 100-101 24.66745134391779 27.0 18.5 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.4020780543113 2306 2 8.641616751798686 2306 3 7.494753931335904 2306 4 9.04749884329214 2306 5 8.93426208305462 2306 6 8.017586521079984 2306 7 8.206239991870167 2306 8 8.690508202733913 2306 9 7.572082789014619 2306 10-11 7.903098284085072 2306 12-13 7.5441770320546055 2306 14-15 8.275859542504755 2306 16-17 7.011973614942473 2306 18-19 6.430885503699244 2306 20-21 6.349402510287202 2306 22-23 5.898873749851877 2306 24-25 6.127927755643466 2306 26-27 6.709249350864482 2306 28-29 5.78133884372529 2306 30-31 5.75600555162454 2306 32-33 5.682469667695056 2306 34-35 4.983988959038673 2306 36-37 5.04494307648509 2306 38-39 4.653819665463391 2306 40-41 3.8292311908793764 2306 42-43 3.7671637706566585 2306 44-45 3.637568325210303 2306 46-47 3.8663127458898465 2306 48-49 3.632864712185288 2306 50-51 3.4855454529353125 2306 52-53 3.960301369831484 2306 54-55 3.5718310387217116 2306 56-57 3.7638391999280465 2306 58-59 3.318987584385386 2306 60-61 3.357594290656767 2306 62-63 2.8615511136444667 2306 64-65 2.5594392538832906 2306 66-67 2.4641789636748683 2306 68-69 2.587400363321141 2306 70-71 2.4411678280248914 2306 72-73 1.7339318865234858 2306 74-75 1.4989757430948565 2306 76-77 1.8581704701650672 2306 78-79 2.1541876801414794 2306 80-81 1.8172135423703288 2306 82-83 1.7657125094090844 2306 84-85 1.298746180333211 2306 86-87 1.3234982337669514 2306 88-89 1.4051746093025699 2306 90-91 1.2640708752231795 2306 92-93 1.1330534362120552 2306 94-95 1.386705731524529 2306 96-97 1.0829698767368612 2306 98-99 0.9453118749399465 2306 100-101 0.8427498763577859 2307 1 8.422701440142056 2307 2 8.57260690220128 2307 3 7.680536581865098 2307 4 9.155669704688805 2307 5 9.168095703351376 2307 6 7.751810707032284 2307 7 8.287967596063208 2307 8 8.702468247297642 2307 9 7.747908863861429 2307 10-11 8.022053378529801 2307 12-13 7.574234129335203 2307 14-15 8.367230383645179 2307 16-17 7.017645362549711 2307 18-19 6.588570215301004 2307 20-21 6.640388482839999 2307 22-23 6.188707648671954 2307 24-25 6.33957192944202 2307 26-27 7.156293522131001 2307 28-29 6.285437251078303 2307 30-31 6.1574833077393905 2307 32-33 6.174455792169663 2307 34-35 5.892299082095331 2307 36-37 5.7147818494632645 2307 38-39 5.3547675942627 2307 40-41 4.73377491904062 2307 42-43 4.360738111062794 2307 44-45 4.40953780158646 2307 46-47 4.73720389391233 2307 48-49 4.620090936421878 2307 50-51 4.388508877759335 2307 52-53 4.852974307492904 2307 54-55 4.573418305141928 2307 56-57 4.852236488123719 2307 58-59 4.486493091551029 2307 60-61 4.485048144406701 2307 62-63 4.214607590577671 2307 64-65 4.087412806828041 2307 66-67 3.7329134549900047 2307 68-69 3.8042158922622455 2307 70-71 3.471154977971718 2307 72-73 2.9874789443044136 2307 74-75 2.6363092621104016 2307 76-77 2.846383553165019 2307 78-79 3.3581898703475836 2307 80-81 3.0767694242764385 2307 82-83 3.0800134158758894 2307 84-85 2.3155427190479543 2307 86-87 2.3216897312095988 2307 88-89 2.5221176892640855 2307 90-91 2.323341270726292 2307 92-93 2.136186823562035 2307 94-95 2.220490293736514 2307 96-97 2.1691279007405058 2307 98-99 1.9328669465964055 2307 100-101 1.661502091884195 1101 1 7.924838394949241 1101 2 7.7347375551697155 1101 3 7.173991175119422 1101 4 8.615636146430951 1101 5 8.501780449035458 1101 6 7.400468505589949 1101 7 7.1018941442020385 1101 8 7.986698056927988 1101 9 7.261055929962435 1101 10-11 7.482626130616236 1101 12-13 7.162781342865728 1101 14-15 7.708799064666465 1101 16-17 6.843653012389137 1101 18-19 6.097442276376007 1101 20-21 5.803121805519911 1101 22-23 5.379077820484046 1101 24-25 5.968069449945549 1101 26-27 6.552055922190796 1101 28-29 5.920605345216689 1101 30-31 5.886589007230604 1101 32-33 5.962606015633636 1101 34-35 5.567193793705666 1101 36-37 5.351270494555038 1101 38-39 5.0550581038739715 1101 40-41 4.8000608549861 1101 42-43 4.353275071540345 1101 44-45 4.305032663461496 1101 46-47 4.610785464706858 1101 48-49 4.234640419387585 1101 50-51 3.8198809839395196 1101 52-53 4.767904832620356 1101 54-55 4.494285671411493 1101 56-57 4.732092737898196 1101 58-59 4.308229892485095 1101 60-61 4.358294551456513 1101 62-63 4.041991947326181 1101 64-65 3.761167420444135 1101 66-67 3.355924316318621 1101 68-69 3.700175853033624 1101 70-71 3.5484952605105953 1101 72-73 3.1547801657528325 1101 74-75 2.5704748935056756 1101 76-77 2.8255152203557614 1101 78-79 3.2528705191921183 1101 80-81 2.807267488619633 1101 82-83 3.027296537257808 1101 84-85 2.34542190803095 1101 86-87 2.404600343042109 1101 88-89 2.4832443652438982 1101 90-91 2.3075417619861973 1101 92-93 2.23066092287592 1101 94-95 2.4803563764413816 1101 96-97 2.3992021696498895 1101 98-99 2.1520820132747502 1101 100-101 1.8129653040950693 2201 1 7.169378063708578 2201 2 7.85103968268297 2201 3 6.440056561812039 2201 4 8.441552143641871 2201 5 4.002710465325766 2201 6 5.656972802576746 2201 7 6.8129195522807 2201 8 7.7204183444296355 2201 9 6.639606428250541 2201 10-11 4.160602900180837 2201 12-13 6.3964974614848416 2201 14-15 7.026192637943012 2201 16-17 5.842173802380099 2201 18-19 2.023388380028514 2201 20-21 4.935954877258556 2201 22-23 5.1350563212013505 2201 24-25 5.3085332959743035 2201 26-27 6.107138074383833 2201 28-29 4.926616647606618 2201 30-31 4.973263209771851 2201 32-33 5.2009665602435255 2201 34-35 4.716887054438825 2201 36-37 4.831673547288759 2201 38-39 4.366719399037837 2201 40-41 4.009379121827159 2201 42-43 3.92026461869758 2201 44-45 3.7574522053213464 2201 46-47 4.119564775829069 2201 48-49 3.879912887208338 2201 50-51 3.620041657196598 2201 52-53 4.496499624477426 2201 54-55 3.999578536378742 2201 56-57 4.091323891252834 2201 58-59 3.792009901652758 2201 60-61 3.8464226404692035 2201 62-63 3.5664704621336654 2201 64-65 3.386305058201465 2201 66-67 2.8941212339050537 2201 68-69 3.2457279397629257 2201 70-71 3.018419746212686 2201 72-73 2.5364636277376675 2201 74-75 2.093577450859801 2201 76-77 2.594985452983284 2201 78-79 2.720431765570364 2201 80-81 2.3582203465081797 2201 82-83 2.7803288337523497 2201 84-85 2.126341734150415 2201 86-87 2.1829708813359723 2201 88-89 2.1760149800125212 2201 90-91 2.0039412435711093 2201 92-93 1.9329825121547657 2201 94-95 2.0693360679725146 2201 96-97 2.121671871245802 2201 98-99 2.0083139626597344 2201 100-101 1.5248775853773964 2304 1 1.4638614343149392 2304 2 4.897791482847296 2304 3 5.793261555047103 2304 4 4.6446796240361685 2304 5 6.802078572943081 2304 6 6.420720704516022 2304 7 7.055685020476194 2304 8 6.39668317531947 2304 9 5.7222740057650405 2304 10-11 6.582819727574286 2304 12-13 6.527307769563521 2304 14-15 6.776545829486075 2304 16-17 5.486645084437168 2304 18-19 5.415894110465821 2304 20-21 5.0220012707195885 2304 22-23 4.879359094194999 2304 24-25 5.028557845028242 2304 26-27 5.846771230086347 2304 28-29 4.972841815434865 2304 30-31 5.0487569473055345 2304 32-33 5.189387124858481 2304 34-35 4.9481732163045 2304 36-37 4.90435271187701 2304 38-39 4.474846906000369 2304 40-41 3.9651264797817234 2304 42-43 3.477101888890605 2304 44-45 3.2991460767589587 2304 46-47 4.274954857373643 2304 48-49 3.9289257276388234 2304 50-51 3.5803114277864836 2304 52-53 4.249433173323464 2304 54-55 3.971991902329016 2304 56-57 4.604245271844867 2304 58-59 3.996963118302812 2304 60-61 4.043040320836003 2304 62-63 3.759387103615854 2304 64-65 3.626792797954927 2304 66-67 3.4292145581697575 2304 68-69 3.569994888805706 2304 70-71 3.5705379157672112 2304 72-73 3.0151702625578665 2304 74-75 2.496097830360547 2304 76-77 2.621809578539473 2304 78-79 3.1260470959763182 2304 80-81 2.9545747190095177 2304 82-83 3.295667841176183 2304 84-85 2.608992258175739 2304 86-87 2.4165438214504995 2304 88-89 2.7337457733335313 2304 90-91 2.620431187160097 2304 92-93 2.3281722160318026 2304 94-95 2.6629632936438092 2304 96-97 2.5259054040844795 2304 98-99 2.3963845405256023 2304 100-101 1.8248932108236602 1102 1 6.838009926014692 1102 2 7.345231218285559 1102 3 6.067650605239358 1102 4 5.865004637272147 1102 5 6.4694929298737165 1102 6 5.858065212308624 1102 7 6.755027674292531 1102 8 6.304944229004743 1102 9 4.571770136801412 1102 10-11 5.843936539727114 1102 12-13 5.829970897117008 1102 14-15 6.0995844717772485 1102 16-17 5.203946959042394 1102 18-19 5.0885589890386385 1102 20-21 5.394109693385591 1102 22-23 5.1647756207362825 1102 24-25 1.8132902152508414 1102 26-27 5.446431472215494 1102 28-29 4.791945455112664 1102 30-31 4.751519725143638 1102 32-33 4.947412316991514 1102 34-35 4.94050997038811 1102 36-37 4.734921394858716 1102 38-39 3.7336463417663346 1102 40-41 3.7655142215943016 1102 42-43 3.7684149542378194 1102 44-45 -4.05828544091262 1102 46-47 1.1288056156664652 1102 48-49 -0.9153986024741663 1102 50-51 2.504143589953422 1102 52-53 -0.287129594519552 1102 54-55 2.449343068504131 1102 56-57 -1.0229139585633504 1102 58-59 2.671356470892345 1102 60-61 3.506308838898743 1102 62-63 3.3233300271492894 1102 64-65 2.9968426456425448 1102 66-67 2.8264282204792934 1102 68-69 3.1413118206741437 1102 70-71 3.2585800131276415 1102 72-73 2.6284598980640297 1102 74-75 2.313420390290684 1102 76-77 2.6849012286196796 1102 78-79 -1.5847299770934171 1102 80-81 1.307857899040986 1102 82-83 2.2785758634374247 1102 84-85 2.1401612476985576 1102 86-87 2.023731286106024 1102 88-89 2.1821897145999856 1102 90-91 1.8859659413241197 1102 92-93 1.8861853464222484 1102 94-95 2.1852366540640844 1102 96-97 2.2020726970724738 1102 98-99 2.1929439942075746 1102 100-101 1.6131109925376776 2202 1 -20.578318968021208 2202 2 -7.578324206731168 2202 3 -0.7918157995678712 2202 4 1.506250014523797 2202 5 1.1862730078302164 2202 6 1.2940722213165543 2202 7 3.789728890478539 2202 8 2.4404653853969656 2202 9 3.1429752390515624 2202 10-11 2.3266635526948427 2202 12-13 1.4753778983935142 2202 14-15 2.581067349021229 2202 16-17 1.654464760036717 2202 18-19 1.4631540862920005 2202 20-21 1.041895846647403 2202 22-23 1.2391326084827874 2202 24-25 2.2369995529110476 2202 26-27 3.488674765868339 2202 28-29 -0.9990331981752263 2202 30-31 0.8123430872216524 2202 32-33 1.5704538169535773 2202 34-35 1.6602075877794853 2202 36-37 2.8575785734159993 2202 38-39 2.4354703548794845 2202 40-41 1.6257498828163115 2202 42-43 0.7129583368429699 2202 44-45 0.547233385825102 2202 46-47 -1.203437528675778 2202 48-49 0.7275139913026116 2202 50-51 1.572483360346439 2202 52-53 1.667182076435875 2202 54-55 -0.9246293217152441 2202 56-57 1.108536949848503 2202 58-59 1.7964426568593161 2202 60-61 1.9288229247179807 2202 62-63 -2.1511132641718547 2202 64-65 -0.11797015260794552 2202 66-67 0.44238007455906114 2202 68-69 1.3939153476360602 2202 70-71 -2.588012340492231 2202 72-73 -4.4183963773890795 2202 74-75 -1.1347096682452076 2202 76-77 -0.0484283958109728 2202 78-79 1.504719500391058 2202 80-81 1.4333928860949392 2202 82-83 -0.8844644134048885 2202 84-85 -0.34343590860939344 2202 86-87 0.4963969630956413 2202 88-89 1.0327422251964364 2202 90-91 1.0305864029720588 2202 92-93 1.200479566445125 2202 94-95 1.4467363829528423 2202 96-97 1.0076290253160884 2202 98-99 0.9752493887997034 2202 100-101 0.6109394436528781 2305 1 5.912626706023058 2305 2 7.23720999696031 2305 3 6.7864338703579605 2305 4 7.822235767733744 2305 5 7.822317462477564 2305 6 6.3342026779051395 2305 7 3.0172456453110783 2305 8 5.953467097541967 2305 9 6.468435595680919 2305 10-11 4.9801501735858515 2305 12-13 5.196327403264494 2305 14-15 5.726960191061103 2305 16-17 5.2812603860238845 2305 18-19 4.5468388748775155 2305 20-21 5.366660400057587 2305 22-23 5.2931150454334706 2305 24-25 5.5477531596805925 2305 26-27 6.319513915922322 2305 28-29 5.318744834675112 2305 30-31 5.478957989161252 2305 32-33 5.612713912319133 2305 34-35 5.376633660997317 2305 36-37 4.761908059102048 2305 38-39 4.675910523265284 2305 40-41 4.294316741403371 2305 42-43 4.0289294016635395 2305 44-45 3.2632775016918565 2305 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-19.746464484747335 1209 48-49 -19.461259181230503 1209 50-51 -21.86063672837669 1209 52-53 -24.34677059046628 1209 54-55 -21.51754932914017 1209 56-57 -25.177227797590565 1209 58-59 -25.468481398244887 1209 60-61 -25.202579406613705 1209 62-63 -25.33373424615702 1209 64-65 -25.210163723784206 1209 66-67 -13.02983599068627 1209 68-69 -24.898207577125508 1209 70-71 -24.75473787917114 1209 72-73 -15.276729560605311 1209 74-75 -16.32735312080285 1209 76-77 -13.419015050844216 1209 78-79 -14.26371298724778 1209 80-81 -23.832414249192947 1209 82-83 -15.622412033239456 1209 84-85 -13.109577478698549 1209 86-87 -13.295534464455363 1209 88-89 -10.646065260190644 1209 90-91 -13.492234660311311 1209 92-93 -17.137145680171166 1209 94-95 -22.55361684912063 1209 96-97 -18.697255759153606 1209 98-99 -10.105652895930408 1209 100-101 -5.220189387368261 1212 1 -20.578318968021208 1212 2 -20.353668608002803 1212 3 -21.253136887522658 1212 4 -23.28004458102548 1212 5 -23.471445112975154 1212 6 -24.52931174194731 1212 7 -24.088052812100056 1212 8 -23.726613385355417 1212 9 -24.648264605526325 1212 10-11 -24.390574172490606 1212 12-13 -24.66785770739949 1212 14-15 -24.98481043268135 1212 16-17 -26.00378320887886 1212 18-19 -20.739141922185567 1212 20-21 -26.365733966139597 1212 22-23 -26.588843371736214 1212 24-25 -22.572981776250785 1212 26-27 -25.33541566154247 1212 28-29 -20.274317045377124 1212 30-31 -15.149800526847077 1212 32-33 -25.459217677218096 1212 34-35 -21.502652582781145 1212 36-37 -25.852947742373473 1212 38-39 -26.120870160839342 1212 40-41 -20.559485016512546 1212 42-43 -16.766665218690157 1212 44-45 -13.937380834288941 1212 46-47 -17.2960802092026 1212 48-49 -20.06192436970057 1212 50-51 -12.188686342434004 1212 52-53 -5.204665327308387 1212 54-55 -3.7774879784241335 1212 56-57 -3.7012879479665024 1212 58-59 -2.685022751628342 1212 60-61 -14.04844406826784 1212 62-63 -11.596140261194616 1212 64-65 -15.941742671152626 1212 66-67 -12.259567748017513 1212 68-69 -8.047831637275884 1212 70-71 -15.384813067141067 1212 72-73 -11.682492860206864 1212 74-75 -8.10619879374352 1212 76-77 -18.93733690870394 1212 78-79 -9.792434458331254 1212 80-81 -7.802339061223023 1212 82-83 -5.283359301632167 1212 84-85 -4.5149956805223965 1212 86-87 -3.789736154934335 1212 88-89 -3.4819370905425764 1212 90-91 -3.7565862265884213 1212 92-93 -3.7064286436774943 1212 94-95 -3.684443916789803 1212 96-97 -13.335611626649397 1212 98-99 -10.294791485197367 1212 100-101 -6.47684377277746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 302.0 3 734.0 4 336.0 5 1995.0 6 2509.0 7 2190.0 8 4280.0 9 7890.0 10 10582.0 11 13975.0 12 19292.0 13 24817.0 14 28544.0 15 30416.0 16 32495.0 17 35620.0 18 39527.0 19 43876.0 20 46483.0 21 51718.0 22 56923.0 23 61605.0 24 64240.0 25 65487.0 26 68984.0 27 72175.0 28 74377.0 29 74409.0 30 76875.0 31 80931.0 32 87618.0 33 101202.0 34 115453.0 35 126472.0 36 134145.0 37 126335.0 38 47841.0 39 160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48743986834599 27.4650025235372 10.786192519957448 18.261365088159362 2 26.43531129598272 29.770423814081326 26.234018012711967 17.56024687722398 3 18.196188938993743 27.13206104376465 31.77301815269169 22.89873186454992 4 22.429347231975274 25.774989485230222 28.796943583396484 22.998719699398016 5 21.118843232931873 28.06670126300666 27.673588337189386 23.14086716687208 6 21.146301143567104 27.645068518277117 27.59679021375118 23.611840124404594 7 23.250598737208797 28.189092096668844 28.60802670730822 19.95228245881414 8 19.84018257911205 26.670632259082673 30.495810331379875 22.9933748304254 9 22.045390539155484 27.193348969057883 28.51474852030028 22.24651197148635 10-11 22.749103120653473 27.66925601632581 27.193562459257592 22.38807840376312 12-13 22.334710439867422 27.674707111275072 27.388430862915936 22.60215158594157 14-15 22.351952104811375 28.04893208505143 27.240594730798712 22.358521079338484 16-17 22.881687684201378 28.105533542527205 26.88584351961683 22.126935253654583 18-19 23.035138074118887 28.173181212265106 26.550212879933017 22.241467833682993 20-21 22.771779662376908 28.20271349616788 26.824343480156948 22.201163361298264 22-23 22.464769187637977 27.85625631322915 27.328037147498165 22.350937351634713 24-25 22.574497677368388 27.826509357113554 27.341823118080278 22.257169847437783 26-27 22.430406149409507 27.96140978386762 27.316631684460308 22.291552382262566 28-29 22.734505562618406 27.90381677099874 27.310315295576064 22.051362370806793 30-31 22.819189762005607 27.657114522150334 27.065904743610396 22.457790972233663 32-33 22.391297432587223 28.03570388007199 27.271754799876476 22.301243887464313 34-35 22.763663180186075 27.73457854078018 27.26035702519295 22.241401253840795 36-37 22.820538532564395 27.785348628768347 27.07042431255327 22.32368852611399 38-39 22.470287675736348 27.856556193014715 27.492741415056017 22.180414716192924 40-41 22.93967878707739 27.77843877787921 27.00769118860486 22.274191246438534 42-43 22.629483186686645 27.924979305572666 27.2166907518614 22.22884675587929 44-45 22.429272969868954 27.6810691043851 27.558368496632784 22.331289429113166 46-47 22.77903672609376 27.865784274694995 27.125834366421003 22.229344632790244 48-49 22.717217668160938 27.687673080430926 27.268935897365033 22.326173354043103 50-51 22.759831341915206 27.732799639373994 27.10029023628932 22.40707878242148 52-53 22.661192922890212 27.797975434737666 27.29962564315044 22.241205999221688 54-55 22.66749479639958 27.55729518378644 27.26839462327045 22.50681539654353 56-57 22.533794131658837 27.82920406669161 27.352609112088615 22.28439268956094 58-59 22.69902393753555 27.672224630747245 27.34252773900114 22.286223692716067 60-61 22.812728679716102 27.361662956127812 27.196100225510722 22.629508138645363 62-63 22.496308876102876 27.717224494457408 27.429209761807886 22.357256867631822 64-65 22.764185860943584 27.540264603067772 27.34033688207801 22.355212653910637 66-67 22.91552518243399 27.52668706682474 27.181751909531073 22.376035841210204 68-69 22.637079704075358 27.63525733863126 27.452003020312844 22.275659936980542 70-71 23.006482729764244 27.529453985959552 27.072473770978462 22.391589513297742 72-73 22.74246971133723 27.684983163181304 27.222595220926422 22.349951904555045 74-75 22.634383553188922 27.536031609827933 27.362257301770843 22.467327535212306 76-77 22.956596431392477 27.742165886366088 27.067632079644927 22.233605602596505 78-79 22.919553366022193 27.541465251433923 27.22765109789537 22.311330284648513 80-81 22.93271013459615 27.51579318960174 27.145185146671082 22.406311529131028 82-83 22.85837048029326 27.629167088245932 27.201253852137686 22.31120857932312 84-85 22.951544652238614 27.309525830922638 27.144532888984372 22.59439662785438 86-87 22.80387613517045 27.607591151575896 27.26773979046368 22.320792922789977 88-89 22.94694623089304 27.49709564640468 27.23689832782951 22.319059794872775 90-91 23.136095660130426 27.339875853937652 26.927402705240617 22.59662578069131 92-93 22.87170153194036 27.553910335507375 27.140391655290873 22.433996477261392 94-95 23.180884464392324 27.405025089263834 26.955704415414395 22.458386030929447 96-97 23.16406074554941 27.460352497707746 26.917770253172186 22.45781650357066 98-99 23.058958735982355 27.477943023116147 27.054929515947627 22.408168724953867 100-101 23.5528019740037 27.300926189236606 26.625042109453958 22.521229727305737 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 127.0 1 136.0 2 153.5 3 167.0 4 166.0 5 197.5 6 246.0 7 288.0 8 401.5 9 534.0 10 721.0 11 975.5 12 1274.0 13 1662.5 14 2178.0 15 2809.0 16 3575.5 17 4562.0 18 5774.0 19 7207.0 20 8912.0 21 11123.0 22 13732.0 23 16432.5 24 19609.5 25 23269.5 26 27039.5 27 30759.0 28 34333.5 29 38031.0 30 41527.0 31 44468.5 32 47371.0 33 50094.5 34 52686.0 35 54693.0 36 56107.5 37 57075.5 38 57079.5 39 56268.0 40 54749.0 41 52511.5 42 49875.5 43 47235.5 44 44406.5 45 41722.0 46 39552.5 47 37872.5 48 36046.0 49 34260.0 50 32768.5 51 31239.5 52 29692.0 53 28741.0 54 28122.0 55 27720.0 56 27691.0 57 27579.5 58 27515.5 59 27196.5 60 26562.0 61 26025.0 62 25371.5 63 24443.0 64 23443.5 65 22402.0 66 21189.0 67 19862.5 68 18483.5 69 16999.0 70 15566.0 71 14285.0 72 12869.5 73 11236.0 74 9719.5 75 8362.5 76 7104.5 77 5892.0 78 4735.5 79 3738.0 80 2937.5 81 2350.5 82 1936.5 83 1518.5 84 1185.5 85 947.5 86 789.5 87 673.5 88 521.5 89 378.0 90 296.0 91 237.5 92 177.0 93 132.5 94 98.0 95 66.0 96 36.0 97 19.5 98 14.5 99 7.0 100 1.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.73293402000095 2 11.067850348071516 3 9.811966632711574 4 11.657599547798931 5 9.16061813180068 6 5.4078075613824215 7 9.784577040865598 8 10.110578656960639 9 5.457676260480475 10-11 6.383766374420086 12-13 5.420492979916664 14-15 7.389733704420473 16-17 2.715934467946266 18-19 3.138154301611785 20-21 3.1011619843377365 22-23 3.179020445621021 24-25 3.5165889809816933 26-27 4.41779930631221 28-29 1.7629730910900347 30-31 2.2912048310438653 32-33 3.0907408448106817 34-35 2.043279919991838 36-37 3.1498030622873143 38-39 1.4178751460187156 40-41 1.1776706079670975 42-43 0.5708438340408978 44-45 1.3283406435899352 46-47 1.3928589550597905 48-49 1.2921667404148705 50-51 1.597680723565361 52-53 2.48876999453845 54-55 1.832047241044231 56-57 1.8499432293420006 58-59 1.5841768909321354 60-61 1.432060990401094 62-63 0.6302879780970563 64-65 1.2635768078903848 66-67 0.569698054302321 68-69 0.8249886922451991 70-71 1.0279008278531416 72-73 0.08503322488437173 74-75 0.10857627046512656 76-77 0.464640964462823 78-79 0.8889068333757999 80-81 0.30993341928500073 82-83 0.5080987531188398 84-85 0.06653706624734766 86-87 0.10178343344356464 88-89 0.08241429976762496 90-91 0.09076212357725529 92-93 0.17844155404833992 94-95 0.47609876184859007 96-97 0.387328112578861 98-99 0.0650912013391437 100-101 0.23161119001229258 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1832813.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.8901882617608 #Duplication Level Percentage of deduplicated Percentage of total 1 99.15626438357754 98.05581652224909 2 0.6586876611969981 1.302754936429401 3 0.13541076492211732 0.4017238810745165 4 0.03180622064319878 0.1258129258920412 5 0.0051001030178533715 0.025217507379494714 6 0.004259355690948077 0.025272509169099455 7 0.003658833383173392 0.025327590545630236 8 0.004812677568166334 0.03807412726072975 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.636828197966732E-4 0.0 0.0 2 2.72804699661122E-4 0.0 0.0 3 3.273656395933464E-4 0.0 0.0 4 3.819265795255708E-4 0.0 0.0 5 4.364875194577952E-4 0.0 0.0 6 4.910484593900196E-4 0.0 0.0 7 4.910484593900196E-4 0.0 0.0 8 5.45609399322244E-4 5.45609399322244E-5 0.0 9 6.001703392544684E-4 5.45609399322244E-5 0.0 10-11 6.001703392544684E-4 5.45609399322244E-5 0.0 12-13 6.001703392544684E-4 5.45609399322244E-5 0.0 14-15 6.001703392544684E-4 5.45609399322244E-5 0.0 16-17 6.001703392544684E-4 5.45609399322244E-5 0.0 18-19 6.001703392544684E-4 5.45609399322244E-5 0.0 20-21 6.001703392544684E-4 5.45609399322244E-5 0.0 22-23 6.001703392544684E-4 5.45609399322244E-5 0.0 24-25 7.092922191189172E-4 5.45609399322244E-5 0.0 26-27 9.002555088817026E-4 5.45609399322244E-5 0.0 28-29 9.275359788478148E-4 5.45609399322244E-5 0.0 30-31 9.54816448813927E-4 5.45609399322244E-5 2.72804699661122E-5 32-33 0.001091218798644488 1.091218798644488E-4 5.45609399322244E-5 34-35 0.0013094625583733857 1.091218798644488E-4 1.091218798644488E-4 36-37 0.0018277914877295174 1.091218798644488E-4 1.636828197966732E-4 38-39 0.0021551571273228636 1.091218798644488E-4 1.636828197966732E-4 40-41 0.002809888406509557 1.091218798644488E-4 1.636828197966732E-4 42-43 0.003819265795255708 1.091218798644488E-4 1.636828197966732E-4 44-45 0.004801362714035747 1.091218798644488E-4 1.636828197966732E-4 46-47 0.00657459326183304 1.091218798644488E-4 1.636828197966732E-4 48-49 0.009466323078240933 1.091218798644488E-4 1.636828197966732E-4 50-51 0.013312869343462753 1.091218798644488E-4 1.909632897627854E-4 52-53 0.01786870782780349 1.091218798644488E-4 2.182437597288976E-4 54-55 0.023652167460619277 1.091218798644488E-4 2.182437597288976E-4 56-57 0.030063077902655645 1.091218798644488E-4 2.72804699661122E-4 58-59 0.03830177983242153 1.091218798644488E-4 2.72804699661122E-4 60-61 0.04935037016869697 1.091218798644488E-4 2.72804699661122E-4 62-63 0.06138105742375245 1.636828197966732E-4 3.273656395933464E-4 64-65 0.07523953616653745 1.636828197966732E-4 3.5464610955945864E-4 66-67 0.09190790331583201 1.636828197966732E-4 3.819265795255708E-4 68-69 0.11176808545116168 1.636828197966732E-4 3.819265795255708E-4 70-71 0.13722076392954435 1.909632897627854E-4 3.819265795255708E-4 72-73 0.16280984475775762 2.182437597288976E-4 3.819265795255708E-4 74-75 0.19039039989349704 2.182437597288976E-4 3.819265795255708E-4 76-77 0.22200846458422108 2.182437597288976E-4 3.819265795255708E-4 78-79 0.25949183031765927 2.182437597288976E-4 3.819265795255708E-4 80-81 0.3054321417405922 2.182437597288976E-4 3.819265795255708E-4 82-83 0.35633749869735754 2.182437597288976E-4 3.819265795255708E-4 84-85 0.40838863539269965 2.182437597288976E-4 3.819265795255708E-4 86-87 0.4698515342263504 2.182437597288976E-4 3.819265795255708E-4 88-89 0.5405625123785133 2.4552422969500976E-4 3.819265795255708E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACGA 250 4.1183986E-4 15.774808 1 GTACGAT 250 0.009562373 13.026355 2 GGTCTCA 920 3.4056234E-4 7.3485126 1 GTCTTAA 790 0.004699975 7.131468 1 GATCCAA 800 0.0053112027 7.0423255 1 GGTTGTT 1030 0.0012355053 6.56372 1 GTTTATA 1200 0.0010257767 6.1033483 1 GGTTTCA 1420 0.0012532473 5.5545096 1 GTTGCAG 1315 0.0047518634 5.3657355 2 GTATAAA 1400 0.0063978615 5.231441 1 GTCCATT 1400 0.0063978615 5.231441 1 GTTTCTT 2055 3.667875E-5 5.2089214 1 CGGAAGA 1700 7.2759576E-12 5.0699577 94-95 GTTTCAT 1445 0.009255891 5.0685244 1 GATCGGA 2015 0.0 5.0653224 94-95 >>END_MODULE