FastQCFastQC Report
Thu 11 Sep 2014
LONLib39_CTATAC_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib39_CTATAC_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832813
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA2504.1183986E-415.7748081
GTACGAT2500.00956237313.0263552
GGTCTCA9203.4056234E-47.34851261
GTCTTAA7900.0046999757.1314681
GATCCAA8000.00531120277.04232551
GGTTGTT10300.00123550536.563721
GTTTATA12000.00102577676.10334831
GGTTTCA14200.00125324735.55450961
GTTGCAG13150.00475186345.36573552
GTATAAA14000.00639786155.2314411
GTCCATT14000.00639786155.2314411
GTTTCTT20553.667875E-55.20892141
CGGAAGA17007.2759576E-125.069957794-95
GTTTCAT14450.0092558915.06852441
GATCGGA20150.05.065322494-95