##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib39_CTATAC_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1832813 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.223922462357045 33.0 33.0 33.0 27.0 33.0 2 31.60919526432866 33.0 33.0 33.0 27.0 33.0 3 31.791875112190933 33.0 33.0 33.0 27.0 33.0 4 35.64388074506237 37.0 37.0 37.0 33.0 37.0 5 35.16498791747985 37.0 37.0 37.0 33.0 37.0 6 34.988470727782925 37.0 37.0 37.0 33.0 37.0 7 34.784723809794016 37.0 33.0 37.0 27.0 37.0 8 34.84211919055572 37.0 33.0 37.0 27.0 37.0 9 34.73887788879716 37.0 33.0 37.0 27.0 37.0 10-11 34.58986759696707 37.0 33.0 37.0 27.0 37.0 12-13 34.59275387068948 37.0 33.0 37.0 27.0 37.0 14-15 35.54571988522561 37.0 33.0 40.0 27.0 40.0 16-17 35.4531436103956 37.0 33.0 40.0 27.0 40.0 18-19 35.65203051266005 37.0 33.0 40.0 27.0 40.0 20-21 35.338498526581816 37.0 33.0 40.0 27.0 40.0 22-23 35.36618820359742 37.0 33.0 40.0 27.0 40.0 24-25 35.21429000121671 37.0 33.0 40.0 27.0 40.0 26-27 34.83943861157685 37.0 33.0 40.0 27.0 40.0 28-29 34.48438820545249 37.0 33.0 40.0 27.0 40.0 30-31 34.377193690791145 37.0 33.0 40.0 27.0 40.0 32-33 34.36253098379376 37.0 33.0 40.0 27.0 40.0 34-35 34.21335046183108 37.0 33.0 40.0 24.5 40.0 36-37 33.904890187924245 37.0 33.0 37.0 22.0 40.0 38-39 33.80863077684412 37.0 33.0 37.0 22.0 40.0 40-41 33.52113881776264 37.0 33.0 37.0 22.0 40.0 42-43 33.156935268355255 37.0 33.0 37.0 22.0 40.0 44-45 33.02918764762144 37.0 33.0 37.0 22.0 40.0 46-47 32.88114553967044 37.0 33.0 37.0 22.0 40.0 48-49 32.903009199520085 37.0 33.0 37.0 22.0 40.0 50-51 33.185004143903384 37.0 33.0 37.0 22.0 40.0 52-53 33.034992113216134 37.0 33.0 37.0 22.0 40.0 54-55 32.67793686535397 37.0 33.0 37.0 22.0 40.0 56-57 32.73391911777143 37.0 33.0 37.0 22.0 40.0 58-59 32.45133982572145 37.0 33.0 37.0 22.0 40.0 60-61 32.36705354010475 37.0 33.0 37.0 22.0 40.0 62-63 32.34103370065577 37.0 30.0 37.0 22.0 40.0 64-65 32.01566962914383 33.0 27.0 37.0 22.0 40.0 66-67 31.675046499561056 33.0 27.0 37.0 18.5 40.0 68-69 31.502403136599312 33.0 27.0 37.0 15.0 40.0 70-71 31.341836019277473 33.0 27.0 37.0 15.0 40.0 72-73 31.124323376143664 33.0 27.0 37.0 15.0 37.0 74-75 30.782197365470452 33.0 27.0 37.0 15.0 37.0 76-77 29.725298762066835 33.0 27.0 37.0 15.0 37.0 78-79 30.653277230137498 33.0 27.0 37.0 15.0 37.0 80-81 30.342653069352956 33.0 27.0 37.0 15.0 37.0 82-83 30.319302623890163 33.0 27.0 37.0 15.0 37.0 84-85 30.197586169456457 33.0 27.0 37.0 15.0 37.0 86-87 29.99309995073147 33.0 27.0 37.0 15.0 37.0 88-89 29.863135519008214 33.0 27.0 37.0 15.0 37.0 90-91 29.774879925011444 33.0 27.0 37.0 15.0 37.0 92-93 29.55492704383917 33.0 27.0 37.0 15.0 37.0 94-95 29.46011677132364 33.0 27.0 33.0 15.0 37.0 96-97 29.330295562067707 33.0 27.0 33.0 15.0 37.0 98-99 29.060991219507937 33.0 27.0 33.0 15.0 37.0 100-101 28.26920940652429 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.1630576289857082 2306 2 0.5672493181193268 2306 3 0.5317974389028528 2306 4 0.34621228502285817 2306 5 0.6818355083870671 2306 6 0.6716886923672476 2306 7 0.8304851856104634 2306 8 0.7632512479374043 2306 9 1.0258890940920153 2306 10-11 1.155894729693152 2306 12-13 1.3643462786538052 2306 14-15 1.5556984859298666 2306 16-17 1.5700961962296205 2306 18-19 1.3871678547051118 2306 20-21 1.740139530620155 2306 22-23 1.297298118405699 2306 24-25 1.3062599162794015 2306 26-27 1.3955188924784991 2306 28-29 1.6771354176238447 2306 30-31 1.6202206628083786 2306 32-33 1.3357679671558316 2306 34-35 1.3177480589569015 2306 36-37 1.3534026548033964 2306 38-39 1.1950517325600245 2306 40-41 1.342249277131188 2306 42-43 1.445232478124403 2306 44-45 1.3502729207238673 2306 46-47 1.1654363910359393 2306 48-49 1.0467692451331203 2306 50-51 1.29743499893668 2306 52-53 1.3046027116720182 2306 54-55 1.683598202898132 2306 56-57 1.245467771797884 2306 58-59 1.264668235793863 2306 60-61 0.9750346650135739 2306 62-63 0.6678146677858692 2306 64-65 0.7838711362461126 2306 66-67 1.1059408928403869 2306 68-69 0.7581426931201669 2306 70-71 0.8404635131651901 2306 72-73 0.49035433935649664 2306 74-75 0.7484669087228077 2306 76-77 0.8231216770304144 2306 78-79 0.8422482597332603 2306 80-81 1.3771209008930825 2306 82-83 1.2596522115101791 2306 84-85 1.0444048794924754 2306 86-87 0.9092769133893164 2306 88-89 0.6909431505355705 2306 90-91 0.3682780959412284 2306 92-93 0.23244645551282517 2306 94-95 0.2068872743574559 2306 96-97 0.24194169972703605 2306 98-99 0.607251872179269 2306 100-101 0.46702457258792407 2307 1 0.590271889379693 2307 2 0.5258265703601737 2307 3 0.4040672773593279 2307 4 0.3827152981387698 2307 5 0.5619470443165753 2307 6 0.7170272248025569 2307 7 0.831163769699188 2307 8 0.709187339495621 2307 9 1.0694007667511585 2307 10-11 1.2210397517218183 2307 12-13 1.3350867708804728 2307 14-15 1.3361230440905985 2307 16-17 1.3430227800144934 2307 18-19 1.1965180091589573 2307 20-21 1.5392298987773358 2307 22-23 1.2386895322913531 2307 24-25 1.3132201507453942 2307 26-27 1.574577103852377 2307 28-29 1.646076585902577 2307 30-31 1.7345693613448674 2307 32-33 1.4040694813098753 2307 34-35 1.421458116180787 2307 36-37 1.4846368296147574 2307 38-39 1.1908754652789142 2307 40-41 1.230970665190135 2307 42-43 1.344400073202138 2307 44-45 1.2920754730102857 2307 46-47 1.1786808079093944 2307 48-49 1.1774216860102058 2307 50-51 1.1909254658430442 2307 52-53 1.4569565502297763 2307 54-55 1.6351544519486225 2307 56-57 1.4249985233044384 2307 58-59 1.3805905910883425 2307 60-61 1.2555769625777629 2307 62-63 1.0682891069411866 2307 64-65 1.162267221727447 2307 66-67 1.3047024135777079 2307 68-69 1.1285309166477973 2307 70-71 1.129269091227684 2307 72-73 0.9009474041258265 2307 74-75 1.1501450117257974 2307 76-77 1.2838644480842447 2307 78-79 1.2162712125868538 2307 80-81 1.9267211566281333 2307 82-83 1.5528575085173202 2307 84-85 1.2303467314193526 2307 86-87 1.407635524823963 2307 88-89 1.3087999768889453 2307 90-91 1.0264404117360328 2307 92-93 1.003436036208626 2307 94-95 0.8517064241017209 2307 96-97 0.9843478247080455 2307 98-99 1.0966432987250698 2307 100-101 1.0408585726892063 1101 1 1.1669228680333426 1101 2 0.5772087221094822 1101 3 0.4202682222485272 1101 4 0.3466176183495051 1101 5 0.5268391656005491 1101 6 0.49950476242027975 1101 7 0.6288430434799324 1101 8 0.6758461020155266 1101 9 0.8640189449140578 1101 10-11 0.8831460156722031 1101 12-13 1.2374283099209364 1101 14-15 1.3321933836939905 1101 16-17 1.3062024829388577 1101 18-19 1.0070301592071473 1101 20-21 1.4195898768175539 1101 22-23 0.9677177797358212 1101 24-25 1.2053245847139777 1101 26-27 1.0725068402834452 1101 28-29 1.3758763897594548 1101 30-31 1.3019906914581156 1101 32-33 0.8818911328297929 1101 34-35 1.0247610400277694 1101 36-37 1.2161333616703658 1101 38-39 0.8329714581342529 1101 40-41 1.1512929675134345 1101 42-43 1.2032965555131625 1101 44-45 0.9939581634443755 1101 46-47 0.9784313762347239 1101 48-49 0.873596927387382 1101 50-51 0.8793456438409919 1101 52-53 1.0690063038265833 1101 54-55 1.5038021920499673 1101 56-57 1.2084979188949632 1101 58-59 1.1292575717730102 1101 60-61 1.0087312648392057 1101 62-63 0.6983160476240045 1101 64-65 0.6398407302922777 1101 66-67 0.845084860443805 1101 68-69 0.7846150584378719 1101 70-71 0.8135888857026821 1101 72-73 0.5719354589840435 1101 74-75 0.8987437224907282 1101 76-77 0.9843008729699356 1101 78-79 0.997716969091023 1101 80-81 1.6650913323063818 1101 82-83 1.4835675672313826 1101 84-85 1.3012244074455595 1101 86-87 1.1467626621653864 1101 88-89 0.9586448472695288 1101 90-91 0.7359323147109151 1101 92-93 0.7632550162128524 1101 94-95 0.8517656085484191 1101 96-97 0.8774682851933235 1101 98-99 0.7598258046145219 1101 100-101 0.8988427059737383 2201 1 1.3542108528873058 2201 2 0.7721715873843848 2201 3 0.27550342090579605 2201 4 0.20240750460170176 2201 5 0.28919230782627636 2201 6 0.3914167554356922 2201 7 0.5078614907528092 2201 8 0.6045103817114779 2201 9 0.5788178703377298 2201 10-11 0.8460965426060341 2201 12-13 1.1468342590261855 2201 14-15 1.051130328990233 2201 16-17 1.0670521128325845 2201 18-19 0.8532621551285047 2201 20-21 1.1538688915289939 2201 22-23 0.9867626893945243 2201 24-25 0.899603785570875 2201 26-27 0.9692366718761107 2201 28-29 1.2858272021163089 2201 30-31 1.3798003631621754 2201 32-33 0.9798256486693475 2201 34-35 1.0176138302576092 2201 36-37 1.0792853005691612 2201 38-39 0.626643669694765 2201 40-41 1.058007434350202 2201 42-43 1.0173198296410462 2201 44-45 0.7420791285154777 2201 46-47 0.8360631006422423 2201 48-49 0.9759290649085415 2201 50-51 0.868414995431749 2201 52-53 0.9934709999195803 2201 54-55 1.4560562389684506 2201 56-57 1.1761107107587492 2201 58-59 1.1183988285081767 2201 60-61 0.702934423986072 2201 62-63 0.6561344216279608 2201 64-65 0.6860674327023162 2201 66-67 0.7875187975519751 2201 68-69 0.749648378473875 2201 70-71 0.9148688812972381 2201 72-73 0.726408650548656 2201 74-75 0.739305133297453 2201 76-77 0.7264356782139636 2201 78-79 0.7758733108468334 2201 80-81 1.1552399720355595 2201 82-83 1.0011347211423924 2201 84-85 0.978576814138215 2201 86-87 1.001509681446695 2201 88-89 1.0025948873812887 2201 90-91 0.7085813105725371 2201 92-93 0.7879806908156084 2201 94-95 0.6994952351225479 2201 96-97 0.6568744837851881 2201 98-99 0.895356822005418 2201 100-101 0.9389268993033717 2304 1 -1.8007954006122269 2304 2 -0.2916544583913634 2304 3 -0.2425985174465879 2304 4 0.05925882275172967 2304 5 0.485384813324238 2304 6 0.5171291994016087 2304 7 0.4221780858734192 2304 8 0.6137139522172816 2304 9 0.897113026216914 2304 10-11 0.8110782736198701 2304 12-13 1.2123876555432247 2304 14-15 1.1841172401467048 2304 16-17 1.124213515555958 2304 18-19 0.906366338497179 2304 20-21 1.1058721202343804 2304 22-23 0.9263321477411637 2304 24-25 0.8965564914465034 2304 26-27 1.025074561109129 2304 28-29 1.4655620942525687 2304 30-31 1.5149497630188336 2304 32-33 0.9563400715706436 2304 34-35 0.8388475995536311 2304 36-37 1.123390239804273 2304 38-39 1.0359175298010257 2304 40-41 0.9755094784441809 2304 42-43 0.8937890533237081 2304 44-45 1.027657655266708 2304 46-47 1.0807168234836908 2304 48-49 1.0631292672256905 2304 50-51 0.9013183494728096 2304 52-53 1.1732748864545925 2304 54-55 1.4683403138988567 2304 56-57 1.0600922704074378 2304 58-59 0.8266793851393786 2304 60-61 0.4569002319893585 2304 62-63 0.36249666829149163 2304 64-65 0.47681962607659756 2304 66-67 0.5124762385522175 2304 68-69 0.7233302948676084 2304 70-71 0.5208680231430804 2304 72-73 0.31986741697198084 2304 74-75 0.6273740506483065 2304 76-77 0.5788377294149498 2304 78-79 0.5354673514577186 2304 80-81 1.3808684271521727 2304 82-83 1.1259928301346136 2304 84-85 0.7940145557846598 2304 86-87 0.5869252452546405 2304 88-89 0.3959924804315662 2304 90-91 0.3039646969205805 2304 92-93 0.4070545618148458 2304 94-95 0.41089332566312464 2304 96-97 0.13298631482180667 2304 98-99 0.38612286407489904 2304 100-101 0.6089570775032023 1102 1 1.381543765819906 1102 2 0.21960692558274886 1102 3 0.3497042495772824 1102 4 0.0778617122342311 1102 5 0.2677160137456909 1102 6 0.14029631240285312 1102 7 -4.828381507589491 1102 8 -1.1242941017201247 1102 9 0.3216144084324952 1102 10-11 0.6533265561366903 1102 12-13 0.9521269077227004 1102 14-15 0.8184089622216106 1102 16-17 0.5106325758146966 1102 18-19 0.8767475271387042 1102 20-21 1.0476568335350294 1102 22-23 0.6708689486438857 1102 24-25 0.9891431415398557 1102 26-27 1.1415185677569895 1102 28-29 1.0100449367055404 1102 30-31 1.3264011476204658 1102 32-33 0.9872744019579329 1102 34-35 1.1744465176791934 1102 36-37 1.3852347019426148 1102 38-39 1.0038381793164675 1102 40-41 1.2902694817057352 1102 42-43 1.2693665421290206 1102 44-45 1.0423711963951803 1102 46-47 0.9451906515689146 1102 48-49 0.9386199565749536 1102 50-51 0.7783398655925211 1102 52-53 0.7268003722403336 1102 54-55 1.2924414040774792 1102 56-57 0.9919121431241109 1102 58-59 0.9252788702795769 1102 60-61 0.8877473683776032 1102 62-63 0.5551054127918391 1102 64-65 0.577033449585123 1102 66-67 0.663885865476125 1102 68-69 0.6647305166383362 1102 70-71 0.45123334739917453 1102 72-73 0.5495091869744648 1102 74-75 0.45204121606740344 1102 76-77 0.4464261925272446 1102 78-79 0.5282536966632883 1102 80-81 1.1414242367600096 1102 82-83 0.9619528490275826 1102 84-85 0.7289414544851844 1102 86-87 0.5281927314935437 1102 88-89 0.46582414585009957 1102 90-91 0.07314690701509363 1102 92-93 0.23633600134812838 1102 94-95 0.250662750986848 1102 96-97 0.12558816421385188 1102 98-99 0.06237291715032356 1102 100-101 0.18712318160167385 2202 1 1.5727753676342928 2202 2 0.9019327634555694 2202 3 0.3715521748859807 2202 4 -0.21860553750739342 2202 5 0.1465496402118731 2202 6 0.5083276234501781 2202 7 0.6549975631953942 2202 8 -3.6526239480713336 2202 9 -0.39499866663576455 2202 10-11 0.6170626919823405 2202 12-13 0.07490782999998657 2202 14-15 0.06635921397360534 2202 16-17 0.11407608742011632 2202 18-19 0.7846823017216167 2202 20-21 1.1466860691649572 2202 22-23 -2.912178274209559 2202 24-25 -2.9283755841575427 2202 26-27 -1.450578156061546 2202 28-29 0.6441146610761237 2202 30-31 1.3470102039835794 2202 32-33 1.1025419328125565 2202 34-35 -2.2486336700921825 2202 36-37 -0.37449426453961365 2202 38-39 0.1963079853374481 2202 40-41 1.217430297612509 2202 42-43 1.287102136365796 2202 44-45 1.3263377225939763 2202 46-47 0.8040472861856145 2202 48-49 0.8720315177563975 2202 50-51 -4.00544007348466 2202 52-53 -0.3656287210740885 2202 54-55 1.4175245220183328 2202 56-57 1.2571915267863005 2202 58-59 1.3713373975820389 2202 60-61 1.3998355446753905 2202 62-63 1.2505316710456 2202 64-65 1.071051616799501 2202 66-67 1.3389441630857064 2202 68-69 1.09309430558514 2202 70-71 1.1740858160454763 2202 72-73 1.1036254348930612 2202 74-75 0.6755251867558911 2202 76-77 1.3322411124200606 2202 78-79 1.2112177862357427 2202 80-81 1.8235955483841266 2202 82-83 1.8084957051594515 2202 84-85 1.5621807357734774 2202 86-87 1.5030306221069587 2202 88-89 1.3065688928269168 2202 90-91 1.0462927300918459 2202 92-93 1.2580002541600237 2202 94-95 1.289943660889758 2202 96-97 1.1061695019836861 2202 98-99 1.2421924844890988 2202 100-101 1.2216975349077934 2305 1 1.363358025242487 2305 2 0.7748181388185458 2305 3 0.1806422031042736 2305 4 -0.04795203921870694 2305 5 0.41003198251720363 2305 6 0.48973010877304546 2305 7 0.6707441088771162 2305 8 0.07799063546189444 2305 9 0.5652736229358837 2305 10-11 0.4510357994366814 2305 12-13 0.8318734432959118 2305 14-15 0.6920101524550759 2305 16-17 0.8189958805223085 2305 18-19 0.7817137071564702 2305 20-21 0.9428073156595786 2305 22-23 0.7248499471615304 2305 24-25 0.8585151349362548 2305 26-27 0.9068529014953768 2305 28-29 -2.4337805330013254 2305 30-31 -0.9613879300223829 2305 32-33 0.08182170896149898 2305 34-35 -0.6670622919824822 2305 36-37 0.06495983910024705 2305 38-39 -2.80428298542531 2305 40-41 -4.517799934599079 2305 42-43 -4.175786522562934 2305 44-45 -1.4819520253129852 2305 46-47 -0.2977360264299804 2305 48-49 0.28729461405466594 2305 50-51 0.7764966309287971 2305 52-53 0.4818325921958575 2305 54-55 0.9162008493808358 2305 56-57 0.3921053308161788 2305 58-59 -4.394180056607361 2305 60-61 -0.6901240290865083 2305 62-63 0.5069466474221684 2305 64-65 0.7369522365784533 2305 66-67 0.8189252505998468 2305 68-69 0.5258247989894613 2305 70-71 0.7897121064619448 2305 72-73 0.3140815662413807 2305 74-75 0.5468478477897989 2305 76-77 0.35244617655028065 2305 78-79 0.660985330628435 2305 80-81 1.0299889778411213 2305 82-83 1.2694786640217757 2305 84-85 1.1685036491160083 2305 86-87 1.2209283771761754 2305 88-89 0.9446645692047433 2305 90-91 1.0427220300171456 2305 92-93 0.9520018357678879 2305 94-95 0.7422395035306764 2305 96-97 0.8572704796855248 2305 98-99 1.1440767087087842 2305 100-101 1.1375961409559459 1103 1 1.247737737897456 1103 2 0.6012225764890964 1103 3 0.1607340099670722 1103 4 -0.23343481012448564 1103 5 -0.0015098805753410716 1103 6 0.21928816442690646 1103 7 0.5172780180068983 1103 8 0.20486975768672266 1103 9 0.10813329536358651 1103 10-11 0.5638419986339755 1103 12-13 0.10457172912178692 1103 14-15 0.2203931753013535 1103 16-17 0.24268742468801463 1103 18-19 0.3893502442736576 1103 20-21 0.5094286391829144 1103 22-23 0.1860488170345036 1103 24-25 0.47509849126031156 1103 26-27 0.6178871719755037 1103 28-29 0.43136847407130574 1103 30-31 0.016747242190540135 1103 32-33 0.09814701362699907 1103 34-35 0.2923012207825124 1103 36-37 0.65139987515726 1103 38-39 -0.20447582380915463 1103 40-41 -3.1827983258394816 1103 42-43 -0.7729230368667075 1103 44-45 0.19961487579995207 1103 46-47 -2.8376612788019244 1103 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-0.23915341029561432 1208 46-47 -0.3494650380578648 1208 48-49 -1.6395741587194337 1208 50-51 -0.3602851472681792 1208 52-53 -1.662624813243454 1208 54-55 -1.3389621276729144 1208 56-57 -6.802374668240219 1208 58-59 -2.384126507091139 1208 60-61 -1.7319715362031936 1208 62-63 6.289758784490118E-4 1208 64-65 -0.22107162927687085 1208 66-67 0.3584086641191675 1208 68-69 0.3771012468417254 1208 70-71 -4.112793893760127 1208 72-73 -0.6906640084804501 1208 74-75 -5.53528849157243 1208 76-77 -1.4810303242576808 1208 78-79 -4.260802013610842 1208 80-81 0.6361675898405892 1208 82-83 0.30643264991408614 1208 84-85 -2.944084046046271 1208 86-87 -1.3574987247521584 1208 88-89 -5.140284889016183 1208 90-91 -0.18298637444235766 1208 92-93 -2.2064053487989845 1208 94-95 -4.957402760809359 1208 96-97 -3.7438545644950594 1208 98-99 -6.617282735606185 1208 100-101 -4.008364828011281 1209 1 1.8608678439477728 1209 2 1.1684608524543876 1209 3 0.5559197367466346 1209 4 0.44072131651064694 1209 5 0.45799381113859994 1209 6 1.1830392615462983 1209 7 0.8932208763555991 1209 8 0.08390565254145343 1209 9 -0.10940936040391591 1209 10-11 -2.8783864100954624 1209 12-13 -0.331753923268586 1209 14-15 -1.36067290821493 1209 16-17 -0.3724227718204247 1209 18-19 -16.602689534168796 1209 20-21 -13.96513851155797 1209 22-23 -3.22563492809147 1209 24-25 -1.9321322973291757 1209 26-27 -1.1331364804727073 1209 28-29 -2.444200406269019 1209 30-31 -0.3102943768333333 1209 32-33 0.18966611464171024 1209 34-35 -3.0493872840635703 1209 36-37 -3.846698581489818 1209 38-39 -1.0940370307616334 1209 40-41 -10.097174556313103 1209 42-43 -4.993174590809815 1209 44-45 -3.081546636587433 1209 46-47 -2.3442404781216553 1209 48-49 -2.6562152998262505 1209 50-51 -2.372584478045816 1209 52-53 -1.2737362978629037 1209 54-55 -3.043146885215954 1209 56-57 -0.9445948406486799 1209 58-59 -2.2325692238354122 1209 60-61 -2.0377720011762506 1209 62-63 -0.7927733280106182 1209 64-65 -0.05466963061881103 1209 66-67 0.05373432678645429 1209 68-69 -0.9094394100638254 1209 70-71 -0.8996323862147335 1209 72-73 -0.4136406428446229 1209 74-75 0.5463150872948219 1209 76-77 -16.48144110872828 1209 78-79 -16.586791425994075 1209 80-81 -21.964398633860128 1209 82-83 -10.080027463653366 1209 84-85 -3.7539486552497294 1209 86-87 -3.1840038027474193 1209 88-89 -2.711817189046169 1209 90-91 -6.041887623469179 1209 92-93 -3.360335231722047 1209 94-95 -0.9432975919171049 1209 96-97 0.0138901682530026 1209 98-99 1.0779857802853243 1209 100-101 0.5657827774043511 1212 1 -28.6158505595999 1212 2 -16.26582219440889 1212 3 -5.640629285476333 1212 4 -1.3426606417108502 1212 5 -0.4982704637392814 1212 6 -2.3295893165508303 1212 7 -0.25863214866815554 1212 8 -1.5175580955204921 1212 9 -0.07036829876978601 1212 10-11 0.8784847033866896 1212 12-13 0.22003575047230584 1212 14-15 -0.22230203646793711 1212 16-17 -0.09299360022180281 1212 18-19 -1.0317177588324569 1212 20-21 -0.8410466855405971 1212 22-23 -0.5279122399965104 1212 24-25 0.3741828252888979 1212 26-27 -0.5727510379306509 1212 28-29 0.0583953291893593 1212 30-31 -0.6309985763565322 1212 32-33 0.2583122310413515 1212 34-35 -1.056535976193004 1212 36-37 -1.3957941585699913 1212 38-39 -0.3073858129165714 1212 40-41 -0.6067105915230755 1212 42-43 0.2370289668648624 1212 44-45 1.1392559241381122 1212 46-47 -1.9563755830680556 1212 48-49 -1.3071580669436038 1212 50-51 -0.31713710128907024 1212 52-53 -1.243667778449069 1212 54-55 -0.26622175642946644 1212 56-57 -1.4953492210564612 1212 58-59 -0.26669055821348664 1212 60-61 0.44642685516637926 1212 62-63 -4.560302461265042 1212 64-65 -3.795936322848277 1212 66-67 -1.7148934510839453 1212 68-69 0.15892162556293954 1212 70-71 -1.037641488793799 1212 72-73 -1.517184980406931 1212 74-75 -0.06095864026131537 1212 76-77 1.0181421065160379 1212 78-79 -1.0474035994432214 1212 80-81 0.43890563568291086 1212 82-83 0.4585234591080827 1212 84-85 -2.8009569742734506 1212 86-87 -1.5377323591717413 1212 88-89 -0.5630783797168597 1212 90-91 -0.02273386323711435 1212 92-93 -2.67699495197612 1212 94-95 -2.0283083449638006 1212 96-97 0.1653964283600331 1212 98-99 -0.41722619644141545 1212 100-101 0.33742140834978684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 1.0 10 3.0 11 11.0 12 36.0 13 201.0 14 617.0 15 1413.0 16 2651.0 17 4206.0 18 6440.0 19 9172.0 20 12365.0 21 16812.0 22 21733.0 23 27686.0 24 34585.0 25 42900.0 26 51914.0 27 62517.0 28 74558.0 29 88404.0 30 103614.0 31 121576.0 32 140149.0 33 160258.0 34 179420.0 35 197262.0 36 208211.0 37 192040.0 38 71833.0 39 223.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.45336794256612 28.469959380133474 12.077251940449111 17.999420736851306 2 25.804172300265066 29.13025970752212 27.682056620801266 17.383511371411547 3 18.130357211058428 26.452878367097515 33.42037636197969 21.996388059864362 4 22.33709731276312 25.16695656728568 30.245546188043292 22.25039993190791 5 20.425324351147662 28.223064764381313 28.349809827843863 23.001801056627162 6 20.430016591981833 27.86454482808666 28.33960693207654 23.365831647854964 7 22.66199552272927 28.42079361069569 29.193103715436326 19.724107151138714 8 19.29984128222574 26.58727322427329 31.17191988489824 22.94096560860273 9 21.616662474567782 27.186843393188504 28.90627685421262 22.29021727803109 10-11 22.13313633196622 27.812029923401898 27.72156788499427 22.333265859637617 12-13 22.00625197646257 27.710490359509144 27.802026029584965 22.481231634443322 14-15 21.905780895268638 28.096947151727974 27.5887938376692 22.408478115334187 16-17 22.357149131685183 28.091071435391584 27.395470216930022 22.156309215993208 18-19 22.667438385203546 28.15626371687362 26.950148493906056 22.22614940401678 20-21 22.295630975790207 28.149259171153396 27.2416374401739 22.313472412882497 22-23 21.96662233589088 27.828160272804777 27.81001875532822 22.39519863597613 24-25 22.29458215322567 27.652111808460546 27.718239667658402 22.33506637065538 26-27 22.24097602974226 27.90813901909251 27.638444293007524 22.212440658157707 28-29 22.45851049725204 27.806764792698434 27.61498308883667 22.119741621212857 30-31 22.606807131987825 27.627341141731314 27.245414562205745 22.520437164075112 32-33 22.134554916404454 27.882768182024027 27.578018052032586 22.404658849538933 34-35 22.570796911632556 27.65909560877187 27.466550051751053 22.30355742784452 36-37 22.63924361077753 27.75916037260757 27.317462283386245 22.28413373322865 38-39 22.221661457006253 27.728769929065322 27.750048695638892 22.299519918289537 40-41 22.647884957026935 27.68016931692689 27.325816139015352 22.346129587030823 42-43 22.26792913406878 27.76906318320527 27.650938748252003 22.312068934473945 44-45 22.196072376178037 27.48684672140584 27.87529333325331 22.44178756916281 46-47 22.485230189653343 27.766834145624014 27.517321151656365 22.23061451306628 48-49 22.495942706570045 27.472016337750137 27.580238109722742 22.45180284595707 50-51 22.452627386363016 27.65753133167104 27.455082632678785 22.434758649287158 52-53 22.35696072068592 27.74655522027794 27.609334194306612 22.287149864729532 54-55 22.353261808089844 27.40485148377096 27.69613038557011 22.545756322569083 56-57 22.257629868934114 27.73163507577694 27.66198680092897 22.348748254359975 58-59 22.360544414594838 27.572744535728777 27.79759053778991 22.269120511886474 60-61 22.561558991572614 27.360512709010298 27.47566599164272 22.60226230777436 62-63 22.211979756474236 27.58296329053595 27.771801743086407 22.433255209903407 64-65 22.478751280515834 27.531574690050377 27.602806154114766 22.386867875319023 66-67 22.56164368612601 27.534034298934362 27.539517981568277 22.364804033371346 68-69 22.367450744268513 27.588938866610047 27.70794325656795 22.335667132553485 70-71 22.695376771703703 27.536094289707535 27.391528887572097 22.377000051016662 72-73 22.350037675993008 27.605763649121194 27.655134073862413 22.389064601023378 74-75 22.279434963978353 27.424351266461933 27.88173174653949 22.41448202302023 76-77 22.462520792178235 27.738015958227365 27.640077181440052 22.159386068154348 78-79 22.55440831772886 27.56194320408903 27.5557232399684 22.327925238213712 80-81 22.367803596255698 27.516218113481727 27.616244608757647 22.49973368150493 82-83 22.441957538290293 27.641498109357183 27.60163910274948 22.314905249603047 84-85 22.374344138496998 27.248262676351448 27.714049841109095 22.663343344042463 86-87 22.2577137160558 27.612885412977846 27.7213333282406 22.408067542725753 88-89 22.43662059826805 27.53087224756619 27.664147487263826 22.36835966690193 90-91 22.548210629259497 27.38621876732723 27.422510445454428 22.643060157958846 92-93 22.22485962643443 27.602654138087207 27.658421595429473 22.514064640048893 94-95 22.45969009337798 27.524239421309737 27.44233178649228 22.573738698820005 96-97 22.463726004568883 27.555740719065575 27.396842052706667 22.583691223658878 98-99 22.277183825645906 27.467972285164322 27.670717849167232 22.584126040022536 100-101 22.54247762379047 27.364497914827844 27.358659159955963 22.734365301425722 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 3.0 2 4.0 3 4.5 4 8.0 5 17.5 6 34.5 7 48.0 8 71.0 9 114.5 10 179.5 11 273.5 12 404.0 13 568.5 14 796.0 15 1161.0 16 1657.0 17 2301.0 18 3213.0 19 4476.5 20 6146.5 21 8189.5 22 10650.0 23 13664.0 24 16902.5 25 20442.5 26 24414.0 27 28400.5 28 32444.0 29 36455.5 30 40063.5 31 43664.5 32 47073.0 33 49890.0 34 52416.0 35 54752.0 36 56866.5 37 58468.5 38 59002.0 39 58075.5 40 56601.0 41 54629.5 42 51983.5 43 49111.0 44 46279.5 45 43513.0 46 40619.0 47 38731.0 48 37204.5 49 35354.0 50 33666.5 51 31974.0 52 30341.5 53 29259.0 54 28799.5 55 28512.0 56 28402.0 57 28435.0 58 28749.0 59 28990.0 60 28803.0 61 28320.0 62 27572.0 63 26700.0 64 25715.0 65 24537.0 66 23303.5 67 22054.5 68 20694.5 69 19210.0 70 17642.0 71 16055.5 72 14319.5 73 12430.5 74 10574.5 75 8582.5 76 6663.0 77 4961.0 78 3485.5 79 2331.5 80 1484.5 81 965.0 82 624.0 83 409.0 84 268.5 85 178.5 86 121.0 87 90.5 88 79.5 89 63.5 90 40.0 91 24.5 92 15.5 93 9.5 94 6.5 95 3.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7234780635012955 2 0.0032190954560012397 3 1.636828197966732E-4 4 5.45609399322244E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.189408302974717 14-15 0.0 16-17 5.45609399322244E-4 18-19 0.12366237035638661 20-21 5.45609399322244E-5 22-23 2.72804699661122E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.002018754777492303 42-43 0.0 44-45 0.0 46-47 0.02419777685994152 48-49 1.36402349830561E-4 50-51 1.636828197966732E-4 52-53 1.909632897627854E-4 54-55 0.0018277914877295172 56-57 0.001773230547797293 58-59 0.09722759495922388 60-61 0.0021005961873906396 62-63 0.0016095477280006199 64-65 0.003028132166238454 66-67 0.005619776813019113 68-69 0.005865301042714123 70-71 0.004283033784679615 72-73 0.04062061977954107 74-75 0.04686784740178076 76-77 0.16905707237999731 78-79 2.72804699661122E-4 80-81 0.12538103996425168 82-83 0.005619776813019113 84-85 0.0059471424526124595 86-87 0.0074202878307825185 88-89 0.007611251120545304 90-91 0.023843130750382063 92-93 0.011075870806241552 94-95 0.01424040532231057 96-97 0.010830346576546543 98-99 0.01203068725505548 100-101 0.012576296654377725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1832813.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.02415469867678 #Duplication Level Percentage of deduplicated Percentage of total 1 98.54523847445945 96.59813701038408 2 1.1308486010685057 2.217009564238428 3 0.1920881696011982 0.5648784135832053 4 0.08983692365475293 0.35224754007946874 5 0.023151339149402306 0.1134695225131272 6 0.004296634837521883 0.02527043987981814 7 0.0036908555783642335 0.025325509872883487 8 0.006473062558571417 0.05076131884924937 9 0.002883176953342652 0.02543588853283078 >10 0.0013002508041682086 0.012745598597486333 >50 1.9251133474465252E-4 0.014719193469441684 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.72804699661122E-4 5.45609399322244E-5 0.0 2 3.273656395933464E-4 5.45609399322244E-5 5.45609399322244E-5 3 3.819265795255708E-4 5.45609399322244E-5 5.45609399322244E-5 4 4.364875194577952E-4 5.45609399322244E-5 5.45609399322244E-5 5 4.910484593900196E-4 5.45609399322244E-5 5.45609399322244E-5 6 5.45609399322244E-4 5.45609399322244E-5 1.091218798644488E-4 7 6.001703392544684E-4 5.45609399322244E-5 1.091218798644488E-4 8 6.547312791866928E-4 5.45609399322244E-5 1.091218798644488E-4 9 7.092922191189172E-4 5.45609399322244E-5 1.091218798644488E-4 10-11 7.092922191189172E-4 5.45609399322244E-5 1.091218798644488E-4 12-13 7.092922191189172E-4 1.091218798644488E-4 1.636828197966732E-4 14-15 7.638531590511416E-4 1.091218798644488E-4 1.636828197966732E-4 16-17 7.638531590511416E-4 1.091218798644488E-4 1.909632897627854E-4 18-19 7.638531590511416E-4 1.091218798644488E-4 2.182437597288976E-4 20-21 7.638531590511416E-4 1.36402349830561E-4 2.182437597288976E-4 22-23 8.18414098983366E-4 1.636828197966732E-4 2.182437597288976E-4 24-25 9.275359788478148E-4 1.636828197966732E-4 2.182437597288976E-4 26-27 0.001227621148475049 1.636828197966732E-4 2.182437597288976E-4 28-29 0.0012549016184411612 1.909632897627854E-4 2.182437597288976E-4 30-31 0.0013913039682717223 2.72804699661122E-4 2.182437597288976E-4 32-33 0.001664108667932844 2.72804699661122E-4 2.72804699661122E-4 34-35 0.002100596187390639 2.72804699661122E-4 2.72804699661122E-4 36-37 0.0027280469966112202 2.72804699661122E-4 2.72804699661122E-4 38-39 0.0031918149860351275 2.72804699661122E-4 2.72804699661122E-4 40-41 0.0037647048553234837 2.72804699661122E-4 2.72804699661122E-4 42-43 0.004883204123934084 3.000851696272342E-4 3.000851696272342E-4 44-45 0.005919861982646347 3.273656395933464E-4 3.273656395933464E-4 46-47 0.007911336290172538 3.273656395933464E-4 3.819265795255708E-4 48-49 0.010612102816817647 3.273656395933464E-4 3.819265795255708E-4 50-51 0.014595051431870027 3.273656395933464E-4 3.819265795255708E-4 52-53 0.019532816495736335 3.273656395933464E-4 3.819265795255708E-4 54-55 0.025780044117976027 3.273656395933464E-4 3.819265795255708E-4 56-57 0.032982088189029646 3.273656395933464E-4 3.819265795255708E-4 58-59 0.04162999716828722 4.0920704949168297E-4 3.819265795255708E-4 60-61 0.05295139220422378 4.364875194577952E-4 3.819265795255708E-4 62-63 0.06539128650877093 4.364875194577952E-4 3.819265795255708E-4 64-65 0.07933160666145428 4.364875194577952E-4 4.0920704949168297E-4 66-67 0.09635461992030829 4.364875194577952E-4 4.910484593900196E-4 68-69 0.1173878622641808 4.364875194577952E-4 4.910484593900196E-4 70-71 0.1431133454422246 4.364875194577952E-4 4.910484593900196E-4 72-73 0.16908435284996343 4.364875194577952E-4 4.910484593900196E-4 74-75 0.19712867597512676 4.364875194577952E-4 5.183289293561317E-4 76-77 0.23087461732320755 4.364875194577952E-4 5.45609399322244E-4 78-79 0.2716316394525792 4.364875194577952E-4 5.45609399322244E-4 80-81 0.3201908759922589 4.364875194577952E-4 5.45609399322244E-4 82-83 0.37396068229546603 4.364875194577952E-4 6.001703392544684E-4 84-85 0.4293127558567077 4.364875194577952E-4 6.274508092205806E-4 86-87 0.49494956659517364 4.910484593900196E-4 6.547312791866928E-4 88-89 0.5714440043801523 4.910484593900196E-4 6.820117491528051E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTAAAC 820 6.0027844E-4 6.9941416 1 GTAAATC 905 0.0018373234 6.337233 1 GTCTTTA 1180 1.4731237E-4 6.0754194 1 GTTTCGC 560 0.0034790095 5.5100384 44-45 GTTTATG 1135 0.0037552326 5.474122 1 ATCGGAA 2000 0.0 5.3386774 94-95 CGTCTGA 890 1.03146485E-5 5.3320847 92-93 GTATATT 1370 0.001108749 5.2328434 1 GATTAAT 1310 0.0035960658 5.1076813 1 >>END_MODULE