FastQCFastQC Report
Thu 11 Sep 2014
LONLib38_CTAGCT_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib38_CTAGCT_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4789648
Sequences flagged as poor quality0
Sequence length101
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG9501.1733755E-68.5704743
ATGATAC14401.2262972E-66.78495842
GGTAGCT12550.00176400135.83414651
GTTCACT15305.402027E-45.521761
ATACGGC13250.00135697265.52041865
GTATGAA15607.013616E-45.41557261
GTATGGA14050.0067022945.2112841
Produced by FastQC (version 0.11.2)