##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib37_CGGAAT_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3396038 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.908606146338762 27.0 22.0 33.0 2.0 33.0 2 24.887228293676337 33.0 15.0 33.0 2.0 33.0 3 25.801526661362445 33.0 22.0 33.0 6.0 33.0 4 27.953970774178618 33.0 22.0 37.0 2.0 37.0 5 28.03706701750687 33.0 22.0 37.0 6.0 37.0 6 29.09491589905649 33.0 27.0 37.0 15.0 37.0 7 28.769626547170557 33.0 27.0 37.0 6.0 37.0 8 28.531077685232027 33.0 27.0 37.0 6.0 37.0 9 29.286914634052977 33.0 27.0 37.0 15.0 37.0 10-11 28.902271264338033 33.0 27.0 37.0 10.5 37.0 12-13 29.19901809108143 33.0 27.0 37.0 10.5 37.0 14-15 29.555822402458396 33.0 27.0 37.0 6.0 40.0 16-17 30.41379410359955 33.0 27.0 37.0 15.0 40.0 18-19 30.739205215018206 33.0 27.0 37.0 15.0 40.0 20-21 30.745813651084 33.0 27.0 37.0 15.0 40.0 22-23 30.762931097944133 33.0 27.0 37.0 15.0 40.0 24-25 30.37092252795758 33.0 27.0 37.0 10.5 40.0 26-27 29.87562403600902 33.0 27.0 37.0 15.0 40.0 28-29 30.064745741949885 33.0 27.0 37.0 15.0 40.0 30-31 29.98735688469917 33.0 27.0 37.0 15.0 40.0 32-33 29.835891706747688 33.0 27.0 37.0 15.0 40.0 34-35 29.86890959406226 33.0 27.0 37.0 15.0 40.0 36-37 30.10828221003416 33.0 27.0 37.0 15.0 40.0 38-39 30.139742547050417 33.0 27.0 37.0 15.0 40.0 40-41 30.21625553070961 33.0 27.0 37.0 15.0 40.0 42-43 30.130427427490506 33.0 27.0 37.0 15.0 40.0 44-45 29.8888258906408 33.0 27.0 37.0 15.0 40.0 46-47 29.52404507841196 33.0 27.0 37.0 15.0 40.0 48-49 29.372665146856427 33.0 24.5 37.0 10.5 40.0 50-51 28.596062382105266 33.0 24.5 37.0 10.5 38.5 52-53 28.318548997390486 33.0 22.0 37.0 10.5 37.0 54-55 29.280352133868938 33.0 24.5 37.0 15.0 40.0 56-57 29.186411783378162 33.0 24.5 37.0 15.0 40.0 58-59 29.370516761002087 33.0 27.0 37.0 15.0 40.0 60-61 29.122570624945894 33.0 24.5 37.0 10.5 40.0 62-63 28.98842445225878 33.0 24.5 37.0 15.0 40.0 64-65 28.941640817917822 33.0 27.0 37.0 15.0 40.0 66-67 28.810177035710435 33.0 22.0 37.0 15.0 40.0 68-69 28.515104807425594 33.0 22.0 37.0 15.0 37.0 70-71 28.414500515011905 33.0 22.0 37.0 15.0 37.0 72-73 28.17912049276245 33.0 22.0 37.0 15.0 37.0 74-75 28.137332532792627 33.0 22.0 37.0 15.0 37.0 76-77 27.746587494015085 33.0 22.0 37.0 15.0 37.0 78-79 27.202158515305186 27.0 22.0 33.0 6.0 37.0 80-81 27.09037457766963 27.0 22.0 33.0 6.0 37.0 82-83 26.68212664287031 27.0 22.0 33.0 6.0 37.0 84-85 26.788692588245482 27.0 22.0 33.0 6.0 37.0 86-87 26.495922601572772 27.0 22.0 33.0 6.0 37.0 88-89 26.147945193781695 27.0 22.0 33.0 6.0 37.0 90-91 26.03488079344224 27.0 22.0 33.0 6.0 37.0 92-93 25.942090165068826 27.0 22.0 33.0 6.0 37.0 94-95 25.740380702453862 27.0 22.0 33.0 6.0 37.0 96-97 25.62826269906285 27.0 22.0 33.0 6.0 37.0 98-99 25.41249847616546 27.0 22.0 33.0 6.0 37.0 100-101 24.938897326826144 27.0 18.5 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.192469849919839 2306 2 8.380770729462654 2306 3 7.388518893065207 2306 4 8.968873364990017 2306 5 8.846453342605091 2306 6 7.968237483689137 2306 7 8.166224694665647 2306 8 8.548199032070155 2306 9 7.483987383797828 2306 10-11 7.813161621336501 2306 12-13 7.475921233973352 2306 14-15 8.15053472210246 2306 16-17 6.849917789649311 2306 18-19 6.2252461393016425 2306 20-21 6.171751975959893 2306 22-23 5.783865920824141 2306 24-25 5.863519433875172 2306 26-27 6.623405939334113 2306 28-29 5.62582242881415 2306 30-31 5.510881576090572 2306 32-33 5.381289009732782 2306 34-35 4.787760020294925 2306 36-37 4.918254093386402 2306 38-39 4.646984129795566 2306 40-41 3.930577396695284 2306 42-43 3.6499077015600854 2306 44-45 3.3239472204297122 2306 46-47 3.6312779829353588 2306 48-49 3.4951898667888806 2306 50-51 3.4319655678051326 2306 52-53 3.9751755222495753 2306 54-55 3.5012738285537353 2306 56-57 3.827976180148678 2306 58-59 3.4158166570006223 2306 60-61 3.332357278966736 2306 62-63 2.980954359925448 2306 64-65 2.5309100886971976 2306 66-67 2.4134020726587977 2306 68-69 2.5929252021267395 2306 70-71 2.716205329074274 2306 72-73 1.8794508961099687 2306 74-75 1.4948104897507832 2306 76-77 1.9304527906910032 2306 78-79 2.080986587534799 2306 80-81 1.8942776028925188 2306 82-83 1.846020058121205 2306 84-85 1.462699682035005 2306 86-87 1.318326569809141 2306 88-89 1.3201008574460182 2306 90-91 1.2427001956811203 2306 92-93 1.2574093122718857 2306 94-95 1.4521877738483617 2306 96-97 1.142283405776535 2306 98-99 1.072861647276035 2306 100-101 0.7611961183451825 2307 1 8.335659083721076 2307 2 8.480348775173578 2307 3 7.620350930804413 2307 4 9.195845656130615 2307 5 8.99913367849005 2307 6 7.547073696905791 2307 7 8.156067019729623 2307 8 8.507218466000463 2307 9 7.574879924164296 2307 10-11 7.805308460159541 2307 12-13 7.417097088561533 2307 14-15 8.28074834391165 2307 16-17 6.867406686468016 2307 18-19 6.504422794965603 2307 20-21 6.420309580066444 2307 22-23 6.063706159976228 2307 24-25 6.272039520354152 2307 26-27 7.065833608623187 2307 28-29 6.2379092567049845 2307 30-31 5.98173320866908 2307 32-33 6.060281276985776 2307 34-35 5.7256798391261725 2307 36-37 5.702619214990968 2307 38-39 5.3114142815116985 2307 40-41 4.744319664345021 2307 42-43 4.432445860332454 2307 44-45 4.4225730244810215 2307 46-47 4.784947392399012 2307 48-49 4.4575557462895326 2307 50-51 4.32625459237671 2307 52-53 4.953052585053868 2307 54-55 4.534347913892262 2307 56-57 4.741888931975392 2307 58-59 4.328196962951342 2307 60-61 4.422481045286588 2307 62-63 4.022904854726111 2307 64-65 3.798929044070899 2307 66-67 3.603632420883187 2307 68-69 3.7826091517839266 2307 70-71 3.417639196053397 2307 72-73 2.8784594605645175 2307 74-75 2.5243877755727127 2307 76-77 2.692726289612235 2307 78-79 3.1506106598585184 2307 80-81 2.863931127834501 2307 82-83 3.052114537603334 2307 84-85 2.304106023982172 2307 86-87 2.205257099163166 2307 88-89 2.4382676091988458 2307 90-91 2.2442723890289145 2307 92-93 2.0124733782212445 2307 94-95 2.1820040482643215 2307 96-97 2.18356537172118 2307 98-99 2.0333291605823014 2307 100-101 1.5932334515917148 1101 1 7.96075304206957 1101 2 7.7755458364555885 1101 3 7.249995616209713 1101 4 8.778946338810677 1101 5 8.503647503819654 1101 6 7.26480197205052 1101 7 7.160488088998914 1101 8 8.01017649254835 1101 9 7.3098047449892825 1101 10-11 7.498297573146534 1101 12-13 7.233838993223827 1101 14-15 7.745649320048482 1101 16-17 6.8346842644787245 1101 18-19 5.902094693586545 1101 20-21 5.879971230420736 1101 22-23 5.440700396339196 1101 24-25 5.984543957460243 1101 26-27 6.532926308791296 1101 28-29 5.880038105040132 1101 30-31 5.6102217661800005 1101 32-33 5.745737117262529 1101 34-35 5.5243138163280605 1101 36-37 5.3395475894390145 1101 38-39 5.027305971515386 1101 40-41 4.488507102701718 1101 42-43 4.325754986460925 1101 44-45 4.254638660100678 1101 46-47 4.630398490548217 1101 48-49 4.478534113130042 1101 50-51 3.950926983045921 1101 52-53 4.893012102211902 1101 54-55 4.577336759914473 1101 56-57 4.648432145923845 1101 58-59 4.151233073478242 1101 60-61 4.424880651572234 1101 62-63 4.022165059350641 1101 64-65 3.7257585991394464 1101 66-67 3.435508776581454 1101 68-69 3.6233485014607574 1101 70-71 3.64136991864034 1101 72-73 3.13985899861321 1101 74-75 2.631241526359691 1101 76-77 2.927295734876086 1101 78-79 3.454297087497924 1101 80-81 3.000631095927478 1101 82-83 3.1849376659019164 1101 84-85 2.4682713095636757 1101 86-87 2.596066138007412 1101 88-89 2.6943715884584485 1101 90-91 2.575077458618111 1101 92-93 2.3121437467742645 1101 94-95 2.48999401440258 1101 96-97 2.3693081518007446 1101 98-99 2.1312359749548406 1101 100-101 1.858856486039862 2201 1 7.052908279701867 2201 2 7.749254704101137 2201 3 6.174252749756757 2201 4 8.369390277820692 2201 5 3.903523847813169 2201 6 5.735932967451056 2201 7 6.705577526612704 2201 8 7.6859896346335255 2201 9 6.463173836793747 2201 10-11 4.1374729333418365 2201 12-13 6.376225513597319 2201 14-15 7.014747527506334 2201 16-17 5.879664193685393 2201 18-19 2.060949372083577 2201 20-21 4.940073050310616 2201 22-23 5.108134165070929 2201 24-25 5.409394907403513 2201 26-27 6.106742318067695 2201 28-29 4.9398366497437 2201 30-31 5.0874255229152645 2201 32-33 5.361726616204052 2201 34-35 5.0576252732458045 2201 36-37 4.985121858044515 2201 38-39 4.492956714470896 2201 40-41 4.117057864007805 2201 42-43 3.8958283057408956 2201 44-45 3.8315779518535855 2201 46-47 4.272248544792689 2201 48-49 4.033582723568973 2201 50-51 3.731515633464994 2201 52-53 4.564645761855903 2201 54-55 4.173378778549893 2201 56-57 4.390505653029972 2201 58-59 4.0119297197657495 2201 60-61 3.8351111869502894 2201 62-63 3.6093494225430476 2201 64-65 3.5101321113770645 2201 66-67 3.2371801071933035 2201 68-69 3.428516019194145 2201 70-71 3.3352362199227343 2201 72-73 2.607047378692993 2201 74-75 2.2906327657325996 2201 76-77 2.603568863434443 2201 78-79 2.828331474994794 2201 80-81 2.4426200168374343 2201 82-83 2.7335115041293285 2201 84-85 2.3245055593213664 2201 86-87 2.247482914157221 2201 88-89 2.4314036157734336 2201 90-91 2.0642370169340793 2201 92-93 1.9422272846913096 2201 94-95 2.060918027733539 2201 96-97 2.092843604290092 2201 98-99 2.0649167816158602 2201 100-101 1.6685520693435265 2304 1 1.5275502132037069 2304 2 4.609699934836453 2304 3 5.6415297239281905 2304 4 4.398032419755165 2304 5 6.485084371681616 2304 6 6.240315001567247 2304 7 7.037570465714662 2304 8 6.36616824432188 2304 9 5.810512288888393 2304 10-11 6.628313796481944 2304 12-13 6.395008527560364 2304 14-15 6.748587933705441 2304 16-17 5.401045083168739 2304 18-19 5.214808776897794 2304 20-21 5.0329962093109515 2304 22-23 4.658653097313632 2304 24-25 4.793979715517398 2304 26-27 5.711027396561789 2304 28-29 5.063333433690278 2304 30-31 4.974374107979845 2304 32-33 5.082802816089007 2304 34-35 4.7370108346088315 2304 36-37 4.833401289353354 2304 38-39 4.3783192028848354 2304 40-41 3.8979018546077597 2304 42-43 3.3747175635809974 2304 44-45 3.1790839117848435 2304 46-47 4.118552451022062 2304 48-49 3.808082194142319 2304 50-51 3.398258828935216 2304 52-53 4.005236057046197 2304 54-55 3.7554203203963326 2304 56-57 4.449656321101198 2304 58-59 3.8710082272561266 2304 60-61 3.8458221789964675 2304 62-63 3.4104626376571687 2304 64-65 3.23462360319191 2304 66-67 3.1449680135010887 2304 68-69 3.249080217609592 2304 70-71 3.259327126631856 2304 72-73 2.7448514060228995 2304 74-75 2.359178136897942 2304 76-77 2.676432467758662 2304 78-79 2.9762804003634926 2304 80-81 2.6921847147925426 2304 82-83 2.8938149390564227 2304 84-85 2.6286417632546524 2304 86-87 2.348646646044539 2304 88-89 2.492522276529723 2304 90-91 2.4369651206395986 2304 92-93 2.19593825645703 2304 94-95 2.5522090515065052 2304 96-97 2.5144508585996377 2304 98-99 2.3590724295580046 2304 100-101 1.8073180234642834 1102 1 7.020036304671539 1102 2 7.446946645678668 1102 3 6.167739449751679 1102 4 6.300002122181326 1102 5 6.558208984272522 1102 6 5.7531816760912236 1102 7 6.762658342101698 1102 8 6.415369035727725 1102 9 4.961388624631304 1102 10-11 6.035156382344898 1102 12-13 5.968913428449113 1102 14-15 6.1431231191816345 1102 16-17 5.267347748979837 1102 18-19 5.382302452853548 1102 20-21 5.464749186358873 1102 22-23 5.167347239469855 1102 24-25 1.864090189858917 1102 26-27 5.443003120198327 1102 28-29 4.921038577719155 1102 30-31 4.835744728620131 1102 32-33 4.984871157781647 1102 34-35 5.014713722388969 1102 36-37 5.021605882824794 1102 38-39 4.064759284338965 1102 40-41 4.103401031227023 1102 42-43 4.114315738502203 1102 44-45 -3.9640450332401684 1102 46-47 1.3883542667157336 1102 48-49 -0.5863850583305492 1102 50-51 2.689303458462991 1102 52-53 -0.1325818366705711 1102 54-55 2.6002485863095686 1102 56-57 -0.5560214047310232 1102 58-59 2.8243005770723535 1102 60-61 3.8183397051656804 1102 62-63 3.3512204344016254 1102 64-65 3.0278099365994535 1102 66-67 3.0828492431484698 1102 68-69 3.419978319195895 1102 70-71 3.324941104570275 1102 72-73 2.7467820947162274 1102 74-75 2.5376619268283633 1102 76-77 2.8516050012177168 1102 78-79 -1.4229776430132297 1102 80-81 1.6539586774092037 1102 82-83 2.588686311052097 1102 84-85 2.357724291213586 1102 86-87 2.3826278392427263 1102 88-89 2.446525208288975 1102 90-91 2.1946448784598047 1102 92-93 2.203440720051489 1102 94-95 2.4810711109514294 1102 96-97 2.4042032332537 1102 98-99 2.3697572269703286 1102 100-101 1.9131328566491987 2202 1 -20.6197461351109 2202 2 -7.650679685669907 2202 3 -0.78624116587741 2202 4 1.584439998527369 2202 5 1.0585121520201746 2202 6 1.58904112463415 2202 7 3.925943490859183 2202 8 2.720442701659504 2202 9 3.2122433401077757 2202 10-11 2.0320369157704015 2202 12-13 1.3656368802158454 2202 14-15 2.652144180084516 2202 16-17 1.6293537854307374 2202 18-19 1.5072704823567626 2202 20-21 1.077973727886011 2202 22-23 1.3023097594907114 2202 24-25 2.304013729707183 2202 26-27 3.5037542287477095 2202 28-29 -0.9930266277649444 2202 30-31 1.001601846453159 2202 32-33 1.5337973204827478 2202 34-35 1.4992868793369851 2202 36-37 2.953960639680872 2202 38-39 2.414154557238188 2202 40-41 1.8832059709867934 2202 42-43 0.6779484028311451 2202 44-45 0.7730479954222993 2202 46-47 -1.2663984075326127 2202 48-49 0.8772256587239013 2202 50-51 1.7315595651714837 2202 52-53 1.6587530547584102 2202 54-55 -0.7945969186224993 2202 56-57 1.0412126245692441 2202 58-59 1.7692824884825633 2202 60-61 1.9971317365358097 2202 62-63 -2.026457783754573 2202 64-65 0.032599779383271965 2202 66-67 0.4044982043156864 2202 68-69 1.4323272214958713 2202 70-71 -2.507536519006333 2202 72-73 -4.712436199221674 2202 74-75 -1.4062632401303006 2202 76-77 -0.3281491863836692 2202 78-79 1.4097790516365762 2202 80-81 1.3683692051994818 2202 82-83 -0.830646898288645 2202 84-85 -0.38440412359078024 2202 86-87 0.4672751139351661 2202 88-89 1.0897688107535046 2202 90-91 1.1396920175792076 2202 92-93 1.105949157542991 2202 94-95 1.5588295879948113 2202 96-97 1.040042971652717 2202 98-99 1.0282060357202312 2202 100-101 0.7853183197454641 2305 1 5.649105736642635 2305 2 7.046587496797219 2305 3 6.61405771444716 2305 4 7.612301238671741 2305 5 7.807090808758861 2305 6 6.206269408706092 2305 7 3.009387026874446 2305 8 6.027343031044012 2305 9 6.447703849796607 2305 10-11 4.798989438655113 2305 12-13 5.304989019226468 2305 14-15 5.632992423559262 2305 16-17 5.07474988174468 2305 18-19 4.497780968009646 2305 20-21 5.197128157319256 2305 22-23 5.04374332532322 2305 24-25 5.299830847791942 2305 26-27 6.096387916785709 2305 28-29 5.167952306416868 2305 30-31 5.359880017774227 2305 32-33 5.329624732465369 2305 34-35 5.207228503565759 2305 36-37 4.728080499616446 2305 38-39 4.529501778098119 2305 40-41 4.008441181834073 2305 42-43 3.962027844892436 2305 44-45 3.0781636447019842 2305 46-47 3.895981191107836 2305 48-49 3.440122149906909 2305 50-51 2.3178091995426158 2305 52-53 3.851157010904924 2305 54-55 4.067071362912035 2305 56-57 4.560358364523545 2305 58-59 4.304666464692406 2305 60-61 4.407859171310619 2305 62-63 3.735123491759815 2305 64-65 3.850339660741213 2305 66-67 3.658723640409221 2305 68-69 4.051146947570327 2305 70-71 3.6633385957888294 2305 72-73 -0.8896858793659312 2305 74-75 1.9856359004337172 2305 76-77 2.975620587995259 2305 78-79 3.5307764898918954 2305 80-81 3.108473277383233 2305 82-83 3.2326013010713304 2305 84-85 2.7840805249851854 2305 86-87 2.690105459240499 2305 88-89 2.905548166344712 2305 90-91 2.6583108441139487 2305 92-93 2.783599268291713 2305 94-95 2.9238029387987865 2305 96-97 2.872481268431251 2305 98-99 2.884853379250792 2305 100-101 2.3947794803699765 1103 1 -20.6197461351109 1103 2 -7.71672873217266 1103 3 -0.6301400515469524 1103 4 2.832217923624942 1103 5 4.692915277680314 1103 6 4.626012737973383 1103 7 3.998875175168319 1103 8 4.360057236927645 1103 9 4.371930253574778 1103 10-11 4.90943298506944 1103 12-13 4.523102441723793 1103 14-15 4.108972532072922 1103 16-17 2.8915563827927713 1103 18-19 3.201622892093308 1103 20-21 2.5643425221220113 1103 22-23 2.625442241322059 1103 24-25 3.015056769273439 1103 26-27 4.307597749967762 1103 28-29 3.832046884732069 1103 30-31 3.6852862145811294 1103 32-33 3.021065528570716 1103 34-35 2.6677950394157293 1103 36-37 2.8775361629002916 1103 38-39 2.4019383685407156 1103 40-41 2.619877895379645 1103 42-43 2.409794716347683 1103 44-45 2.779634081856539 1103 46-47 2.1685279411652445 1103 48-49 -1.0090489143021273 1103 50-51 -1.8592956565979009 1103 52-53 1.8837842533797264 1103 54-55 2.542412027512583 1103 56-57 3.4357636702273133 1103 58-59 2.805254162693629 1103 60-61 3.197139479259345 1103 62-63 3.0578597164923096 1103 64-65 2.94497304198768 1103 66-67 2.5443784212738834 1103 68-69 2.9602514515358713 1103 70-71 2.8509488698020107 1103 72-73 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1209 52-53 -24.563584167672904 1209 54-55 -21.73384986547505 1209 56-57 -25.4313127800224 1209 58-59 -25.700796548024773 1209 60-61 -25.444908158082182 1209 62-63 -25.576984693803503 1209 64-65 -25.447170638381124 1209 66-67 -13.22713521683599 1209 68-69 -25.12205742283985 1209 70-71 -24.940637761008592 1209 72-73 -15.329575590152096 1209 74-75 -16.491542040008582 1209 76-77 -13.33482589944191 1209 78-79 -14.207209693468686 1209 80-81 -24.0479838245333 1209 82-83 -15.716929069430307 1209 84-85 -12.962702666580395 1209 86-87 -13.207718029427609 1209 88-89 -10.699278987515221 1209 90-91 -13.777265096907618 1209 92-93 -17.519003546674693 1209 94-95 -22.851407521527204 1209 96-97 -18.663247563585394 1209 98-99 -10.098964565421674 1209 100-101 -5.642092163602413 1212 1 -20.6197461351109 1212 2 -20.365019166287144 1212 3 -21.3108930288808 1212 4 -23.294558872379667 1212 5 -23.51280888674535 1212 6 -24.58201645910691 1212 7 -24.14518936574601 1212 8 -23.787272766914075 1212 9 -24.688961025718346 1212 10-11 -24.423740276551765 1212 12-13 -24.70163824210256 1212 14-15 -25.044600268541753 1212 16-17 -26.083856602224515 1212 18-19 -20.723786257490488 1212 20-21 -26.501062779453093 1212 22-23 -26.690539318416704 1212 24-25 -22.79233907827248 1212 26-27 -25.443554766359554 1212 28-29 -20.25880651808339 1212 30-31 -15.05045854800868 1212 32-33 -25.616372043461553 1212 34-35 -21.73780739715343 1212 36-37 -26.042652081433168 1212 38-39 -26.325683606103926 1212 40-41 -20.87812453540493 1212 42-43 -17.240833131540285 1212 44-45 -14.458728364093712 1212 46-47 -17.423494168536955 1212 48-49 -20.257288859021585 1212 50-51 -12.195460713535224 1212 52-53 -5.229296115299743 1212 54-55 -3.7995720422344625 1212 56-57 -3.943996904408653 1212 58-59 -2.7695363188922038 1212 60-61 -14.197772315201657 1212 62-63 -11.839668818189754 1212 64-65 -16.33055852708816 1212 66-67 -12.388346346132227 1212 68-69 -7.9309772264405005 1212 70-71 -15.374762147260638 1212 72-73 -11.793159878204467 1212 74-75 -8.235557997455391 1212 76-77 -19.024832036921453 1212 78-79 -9.72981607644741 1212 80-81 -7.726789061849178 1212 82-83 -5.198670067793646 1212 84-85 -4.51717279751329 1212 86-87 -3.265666065434157 1212 88-89 -3.11662759635319 1212 90-91 -3.2777765535360928 1212 92-93 -3.360912302812171 1212 94-95 -3.1447790436221297 1212 96-97 -13.172095763258062 1212 98-99 -10.386710064603344 1212 100-101 -6.651144945926475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 542.0 3 1202.0 4 536.0 5 3278.0 6 4401.0 7 3789.0 8 7327.0 9 14086.0 10 18675.0 11 23899.0 12 32802.0 13 42285.0 14 48805.0 15 52677.0 16 56681.0 17 61920.0 18 70287.0 19 77885.0 20 84118.0 21 93987.0 22 103795.0 23 112747.0 24 118381.0 25 122427.0 26 129599.0 27 135608.0 28 137676.0 29 139676.0 30 144709.0 31 154216.0 32 169434.0 33 195467.0 34 221328.0 35 244021.0 36 256464.0 37 232285.0 38 78816.0 39 207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.05999343411021 24.658253609536615 9.966053532435087 18.31569942391809 2 23.264918799607866 27.46498239286486 30.4683094704374 18.801789337089875 3 18.983175431268453 27.0136015811276 31.528097531540773 22.47512545606317 4 21.951417532119237 26.776224111289014 29.45370344490717 21.818654911684572 5 20.272077223631513 29.337671152842105 28.64333303014776 21.746918593378624 6 20.43859657300977 28.733153521542143 28.837696706050608 21.99055319939748 7 22.612199821802918 28.369757316423616 29.264065597375584 19.753977264397886 8 20.06348367915083 27.662599706017808 30.24509302408438 22.02882359074698 9 21.62468887146531 28.159919833571312 28.765304507091226 21.450086787872152 10-11 22.05068844971719 28.503942302314606 27.899997423537183 21.545371824431026 12-13 21.6556426713991 28.424039867394818 28.174837548079328 21.74547991312675 14-15 21.459069721435924 28.766881655078947 28.22216284280697 21.55188578067816 16-17 21.886819442430408 28.80506566380007 27.95608068517591 21.352034208593608 18-19 22.081871952277567 28.743644197415826 27.611247514266918 21.563236336039687 20-21 21.77402450479439 28.799664860542855 27.873444147271798 21.552866487390958 22-23 21.712740654992576 28.50699971181591 28.170040163377273 21.610219469814236 24-25 21.667823587646524 28.54957182740854 28.255334696832822 21.52726988811212 26-27 21.51175452930204 28.60949135482855 28.323795262259015 21.554958853610398 28-29 21.90550242622102 28.54098427717674 28.110703348609068 21.442809947993176 30-31 21.89008726277813 28.443452942871623 28.012047376470576 21.65441241787967 32-33 21.5196014438855 28.626183630904496 28.21466412053833 21.639550804671682 34-35 21.798765170459315 28.56387795413074 28.178742830158892 21.45861404525105 36-37 21.859479685623473 28.567177860231453 28.04376350075215 21.529578953392924 38-39 21.714263703914266 28.520913489280087 28.28923726747466 21.47558553933099 40-41 21.919757158017102 28.482347837952016 28.057883814089674 21.540011189941207 42-43 21.789645468043506 28.54360589537866 28.081899637146197 21.58484899943164 44-45 21.584520292969252 28.47546135023256 28.41133481595193 21.52868354084626 46-47 21.89143445214004 28.617405469236456 28.04554528752145 21.44561479110205 48-49 21.838611022257783 28.50423032928927 28.12118885056851 21.535969797884434 50-51 21.69946258085929 28.535653619147205 28.13775315992314 21.62713064007037 52-53 21.786072072069352 28.564996287582474 28.161875592730212 21.487056047617962 54-55 21.706211533903797 28.4719148996095 28.207706507562357 21.614167058924345 56-57 21.58728531345663 28.519654190216364 28.39151265024854 21.501547846078463 58-59 21.870064608758078 28.52932818856186 28.113528954295287 21.48707824838478 60-61 21.827071505056796 28.348608160312992 28.125762161674846 21.698558172955362 62-63 21.632372711539134 28.507235325380787 28.24686078182871 21.613531181251375 64-65 21.814037951949476 28.509965010363324 28.18713969856917 21.48885733911803 66-67 21.928017942874387 28.412023929163137 28.12331150690323 21.536646621059244 68-69 21.767962361043086 28.419460646808258 28.26727221455393 21.54530477759473 70-71 22.010408279269793 28.427731734573413 27.997607928043905 21.564252058112892 72-73 21.84334645179657 28.466097603959938 28.09121349812654 21.59934244611695 74-75 21.719162977252754 28.485871604416417 28.207844623242046 21.58712079508878 76-77 21.993880053847953 28.536279104098107 27.99285912601063 21.476981716043316 78-79 22.050243293522115 28.403388395527813 28.000232818188447 21.546135492761625 80-81 21.946609829762448 28.377689792465073 28.068369657420828 21.60733072035165 82-83 22.018881841634304 28.44328012257439 28.034585390358142 21.503252645433165 84-85 22.056753596012054 28.296004306640082 28.003311304134655 21.64393079321321 86-87 21.89386697839782 28.458554877328073 28.12611729945792 21.521460844816186 88-89 22.116578858376805 28.407287044329493 28.030645842189678 21.445488255104017 90-91 22.216670058382206 28.296176487881258 27.800386758722883 21.68676669501365 92-93 22.033549859203617 28.400445734770702 27.96590447554638 21.600099930479296 94-95 22.236762027445035 28.413010989401045 27.82848222624663 21.52174475690729 96-97 22.270367485912818 28.38378315272086 27.801335640680442 21.544513720685877 98-99 22.239099483054904 28.4494976698576 27.796833858607386 21.514568988480114 100-101 22.599131430946045 28.361027106738902 27.46511328305024 21.574728179264813 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 208.0 1 242.5 2 282.5 3 292.5 4 337.5 5 403.0 6 486.0 7 608.5 8 822.5 9 1135.0 10 1512.0 11 2044.0 12 2776.0 13 3690.0 14 4724.5 15 5924.0 16 7545.5 17 9367.5 18 11589.0 19 14626.5 20 18071.5 21 22185.0 22 27235.0 23 32775.5 24 39203.5 25 46711.5 26 54314.5 27 62452.0 28 70801.0 29 78878.0 30 86478.0 31 93171.0 32 99188.5 33 103760.5 34 106877.0 35 108351.0 36 108314.5 37 107750.5 38 105853.0 39 102687.0 40 99102.0 41 95203.5 42 91516.5 43 87691.5 44 84302.0 45 81655.0 46 79118.0 47 77073.0 48 74778.0 49 71986.0 50 69234.5 51 66475.0 52 63453.0 53 60293.5 54 57392.0 55 54290.5 56 51232.5 57 48213.5 58 45054.5 59 42136.5 60 39431.0 61 36841.5 62 34440.5 63 32317.5 64 30727.5 65 29177.0 66 27540.0 67 26173.0 68 24660.5 69 22926.5 70 21222.5 71 19522.5 72 17659.5 73 15630.0 74 13491.5 75 11375.5 76 9517.0 77 7914.5 78 6416.0 79 5212.0 80 4215.0 81 3284.0 82 2641.5 83 2134.5 84 1705.0 85 1392.0 86 1086.5 87 858.0 88 676.0 89 548.0 90 428.0 91 316.0 92 254.0 93 185.0 94 125.0 95 90.5 96 56.0 97 37.5 98 23.0 99 9.5 100 4.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.534889774496046 2 10.911538681251505 3 9.624038364706166 4 11.503817095097286 5 9.028785896977595 6 5.248145044313403 7 9.611288212911633 8 9.913581650146435 9 5.321701347275855 10-11 6.28320413375822 12-13 5.323232543334321 14-15 7.2672478929858855 16-17 2.631993517151457 18-19 3.01800215427507 20-21 3.0704603423165464 22-23 3.135241714020868 24-25 3.4516692687184305 26-27 4.275747208953493 28-29 1.7451070924412508 30-31 2.2128433191854744 32-33 3.007857980387734 34-35 1.9751398541476863 36-37 3.017383786636074 38-39 1.3830086706921418 40-41 1.161706671126766 42-43 0.5530709609256433 44-45 1.2518116699518675 46-47 1.3289456713970809 48-49 1.2241029105092462 50-51 1.56407260460572 52-53 2.458938916466777 54-55 1.7232139334129946 56-57 1.7748181851910965 58-59 1.555871871869514 60-61 1.3601437910883212 62-63 0.6041157372208438 64-65 1.233849562342942 66-67 0.568942396993202 68-69 0.800579969953222 70-71 1.0040818153389333 72-73 0.08409799890342805 74-75 0.10487220696588201 76-77 0.4646443885492448 78-79 0.8422608934293432 80-81 0.28681952322088267 82-83 0.4966228293087415 84-85 0.06504638640674809 86-87 0.09926272909784872 88-89 0.08113866805966247 90-91 0.08735179052766782 92-93 0.16728316938738613 94-95 0.44995079560358275 96-97 0.3722867647535157 98-99 0.06637145991888195 100-101 0.23031249944788607 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3396038.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.71041306161129 #Duplication Level Percentage of deduplicated Percentage of total 1 99.04788010157517 97.77057157703436 2 0.7416432898865194 1.4641583097814133 3 0.11846433298763434 0.3508098972683295 4 0.06036092360786466 0.23833006808450743 5 0.02544506070388598 0.12558462262291797 6 0.004245836522379166 0.02514649661496736 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.001960454716537076 0.025399028593519675 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.1778431218967515E-4 2.9446078047418787E-5 0.0 2 1.1778431218967515E-4 2.9446078047418787E-5 2.9446078047418787E-5 3 1.1778431218967515E-4 2.9446078047418787E-5 2.9446078047418787E-5 4 1.1778431218967515E-4 5.8892156094837574E-5 5.8892156094837574E-5 5 1.1778431218967515E-4 5.8892156094837574E-5 8.833823414225636E-5 6 1.1778431218967515E-4 5.8892156094837574E-5 8.833823414225636E-5 7 1.4723039023709393E-4 5.8892156094837574E-5 8.833823414225636E-5 8 1.4723039023709393E-4 5.8892156094837574E-5 8.833823414225636E-5 9 1.4723039023709393E-4 5.8892156094837574E-5 1.1778431218967515E-4 10-11 1.7667646828451272E-4 5.8892156094837574E-5 1.3250735121338454E-4 12-13 2.061225463319315E-4 5.8892156094837574E-5 1.7667646828451272E-4 14-15 2.061225463319315E-4 5.8892156094837574E-5 1.7667646828451272E-4 16-17 2.061225463319315E-4 5.8892156094837574E-5 1.7667646828451272E-4 18-19 2.2084558535564092E-4 7.361519511854696E-5 1.7667646828451272E-4 20-21 2.502916634030597E-4 8.833823414225636E-5 1.7667646828451272E-4 22-23 2.9446078047418785E-4 8.833823414225636E-5 2.061225463319315E-4 24-25 2.9446078047418785E-4 1.1778431218967515E-4 2.2084558535564092E-4 26-27 3.827990146164442E-4 1.1778431218967515E-4 2.355686243793503E-4 28-29 5.005833268061194E-4 1.1778431218967515E-4 2.502916634030597E-4 30-31 7.508749902091791E-4 1.1778431218967515E-4 2.9446078047418785E-4 32-33 0.0010306127316596575 1.1778431218967515E-4 2.9446078047418785E-4 34-35 0.001310350473110136 1.3250735121338454E-4 2.9446078047418785E-4 36-37 0.0015900882145606144 1.6195342926080333E-4 3.0918381949789726E-4 38-39 0.002031779385271896 2.061225463319315E-4 3.386298975453161E-4 40-41 0.00291516172669446 2.355686243793503E-4 3.5335293656902543E-4 42-43 0.003931051419330408 2.355686243793503E-4 3.5335293656902543E-4 44-45 0.005638923946080697 2.355686243793503E-4 3.5335293656902543E-4 46-47 0.007817933721589688 2.355686243793503E-4 3.827990146164442E-4 48-49 0.011557585633611873 2.355686243793503E-4 3.827990146164442E-4 50-51 0.015444467935871155 2.650147024267691E-4 3.827990146164442E-4 52-53 0.02112756099902298 2.650147024267691E-4 3.827990146164442E-4 54-55 0.027752928559692205 2.650147024267691E-4 3.975220536401536E-4 56-57 0.03686648971536832 2.650147024267691E-4 4.12245092663863E-4 58-59 0.04807072241241117 2.7973774145047844E-4 4.269681316875724E-4 60-61 0.06133618057277333 3.2390685852160667E-4 4.416911707112818E-4 62-63 0.07784070731835156 3.2390685852160667E-4 4.416911707112818E-4 64-65 0.09748124137597988 3.386298975453161E-4 4.564142097349912E-4 66-67 0.12075836607246443 4.12245092663863E-4 4.8586028778240995E-4 68-69 0.14932106177846066 4.711372487587006E-4 5.005833268061194E-4 70-71 0.1832429436890871 4.711372487587006E-4 5.005833268061194E-4 72-73 0.21950578880448335 4.711372487587006E-4 5.594754829009569E-4 74-75 0.2585660113343844 4.711372487587006E-4 5.594754829009569E-4 76-77 0.3059300278736575 4.711372487587006E-4 6.036445999720851E-4 78-79 0.3611414242125677 4.711372487587006E-4 6.330906780195039E-4 80-81 0.4254074895510592 4.8586028778240995E-4 6.478137170432133E-4 82-83 0.4963725376453385 5.005833268061194E-4 6.478137170432133E-4 84-85 0.5713864214711379 5.300294048535382E-4 6.478137170432133E-4 86-87 0.6577812144622646 5.300294048535382E-4 6.478137170432133E-4 88-89 0.7548649337846043 5.447524438772476E-4 6.478137170432133E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACGGC 635 1.1615995E-4 9.049997 5 GTAGCTC 815 8.63093E-6 8.982893 1 GTCGCGT 1010 1.2620019E-4 7.2485714 1 GTTAAAG 1640 5.6419467E-6 6.1810055 1 GTCCCAA 1105 0.0027409105 6.115746 1 TTCGGTC 1000 0.009679108 5.9676905 3 GTTTTCG 1990 3.6167476E-6 5.659882 1 GTTCTAT 1705 6.709611E-5 5.6150675 1 GTCACTT 1305 0.002810948 5.6100054 1 GTACCAT 1210 0.0075170235 5.5850406 1 GTACAAT 1715 7.331208E-5 5.5823264 1 GTCTTCG 1325 0.0033658447 5.5253263 1 GTCCATG 1535 5.6504173E-4 5.5031753 1 GTCAAAT 2560 6.45814E-8 5.4995923 1 GGACCAA 1240 0.009842169 5.4499183 1 GTCATGA 1760 1.08414795E-4 5.4395967 1 GTTTCGA 1555 6.724639E-4 5.4323945 1 GTAAACT 1685 3.409889E-4 5.3474965 1 GGCTCAT 1690 3.556411E-4 5.3316755 1 GTATTTC 2680 1.7335697E-7 5.253342 1 >>END_MODULE