FastQCFastQC Report
Thu 11 Sep 2014
LONLib37_CGGAAT_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib37_CGGAAT_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396038
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC6351.1615995E-49.0499975
GTAGCTC8158.63093E-68.9828931
GTCGCGT10101.2620019E-47.24857141
GTTAAAG16405.6419467E-66.18100551
GTCCCAA11050.00274091056.1157461
TTCGGTC10000.0096791085.96769053
GTTTTCG19903.6167476E-65.6598821
GTTCTAT17056.709611E-55.61506751
GTCACTT13050.0028109485.61000541
GTACCAT12100.00751702355.58504061
GTACAAT17157.331208E-55.58232641
GTCTTCG13250.00336584475.52532631
GTCCATG15355.6504173E-45.50317531
GTCAAAT25606.45814E-85.49959231
GGACCAA12400.0098421695.44991831
GTCATGA17601.08414795E-45.43959671
GTTTCGA15556.724639E-45.43239451
GTAAACT16853.409889E-45.34749651
GGCTCAT16903.556411E-45.33167551
GTATTTC26801.7335697E-75.2533421
Produced by FastQC (version 0.11.2)