##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib37_CGGAAT_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3396038 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.232404937753934 33.0 33.0 33.0 27.0 33.0 2 31.613623286900793 33.0 33.0 33.0 27.0 33.0 3 31.819011742507005 33.0 33.0 33.0 27.0 33.0 4 35.65458484269022 37.0 37.0 37.0 33.0 37.0 5 35.18823523176125 37.0 37.0 37.0 33.0 37.0 6 35.00584858002178 37.0 37.0 37.0 33.0 37.0 7 34.81021590453346 37.0 33.0 37.0 27.0 37.0 8 34.85088623861099 37.0 33.0 37.0 27.0 37.0 9 34.74540479229031 37.0 33.0 37.0 27.0 37.0 10-11 34.59716543218892 37.0 33.0 37.0 27.0 37.0 12-13 34.60720521972958 37.0 33.0 37.0 27.0 37.0 14-15 35.564598511559645 37.0 33.0 40.0 27.0 40.0 16-17 35.47415606068012 37.0 33.0 40.0 27.0 40.0 18-19 35.690313535949834 37.0 33.0 40.0 27.0 40.0 20-21 35.37643277254259 37.0 33.0 40.0 27.0 40.0 22-23 35.41368088342946 37.0 33.0 40.0 27.0 40.0 24-25 35.259398451960784 37.0 33.0 40.0 27.0 40.0 26-27 34.89954794380982 37.0 33.0 40.0 27.0 40.0 28-29 34.54506913644664 37.0 33.0 40.0 27.0 40.0 30-31 34.45809852539931 37.0 33.0 40.0 27.0 40.0 32-33 34.463964478607124 37.0 33.0 40.0 27.0 40.0 34-35 34.33299553774133 37.0 33.0 40.0 24.5 40.0 36-37 34.04219372692532 37.0 33.0 38.5 22.0 40.0 38-39 33.95752079334801 37.0 33.0 37.0 22.0 40.0 40-41 33.68444993842825 37.0 33.0 37.0 22.0 40.0 42-43 33.32597515104366 37.0 33.0 37.0 22.0 40.0 44-45 33.20663682208503 37.0 33.0 37.0 22.0 40.0 46-47 33.07904991051337 37.0 33.0 37.0 22.0 40.0 48-49 33.08123834303385 37.0 33.0 37.0 22.0 40.0 50-51 33.36383029282947 37.0 33.0 37.0 22.0 40.0 52-53 33.21758089868253 37.0 33.0 37.0 22.0 40.0 54-55 32.857038849388616 37.0 33.0 37.0 22.0 40.0 56-57 32.896102458217484 37.0 33.0 37.0 22.0 40.0 58-59 32.60177992707973 37.0 33.0 37.0 22.0 40.0 60-61 32.50147127328964 37.0 33.0 37.0 22.0 40.0 62-63 32.47215637751992 37.0 33.0 37.0 22.0 40.0 64-65 32.142505619784 33.0 27.0 37.0 22.0 40.0 66-67 31.7908291956686 33.0 27.0 37.0 22.0 40.0 68-69 31.60458496047453 33.0 27.0 37.0 22.0 40.0 70-71 31.44142262247949 33.0 27.0 37.0 22.0 38.5 72-73 31.22318581240846 33.0 27.0 37.0 18.5 37.0 74-75 30.876145820512022 33.0 27.0 37.0 15.0 37.0 76-77 29.809515676797492 33.0 27.0 37.0 15.0 37.0 78-79 30.73639414517741 33.0 27.0 37.0 18.5 37.0 80-81 30.453473135459614 33.0 27.0 37.0 15.0 37.0 82-83 30.43359747446878 33.0 27.0 37.0 15.0 37.0 84-85 30.327246632693743 33.0 27.0 37.0 15.0 37.0 86-87 30.133352748114127 33.0 27.0 37.0 15.0 37.0 88-89 30.004727862291293 33.0 27.0 37.0 15.0 37.0 90-91 29.92874108004681 33.0 27.0 37.0 15.0 37.0 92-93 29.724459796975182 33.0 27.0 37.0 15.0 37.0 94-95 29.64111635382172 33.0 27.0 33.0 15.0 37.0 96-97 29.515430333818408 33.0 27.0 33.0 15.0 37.0 98-99 29.2619347898934 33.0 27.0 33.0 15.0 37.0 100-101 28.468816456117395 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.169822514875733 2306 2 0.5057914446054923 2306 3 0.4635000538568903 2306 4 0.28193691510814034 2306 5 0.5898480361343843 2306 6 0.6841431075130444 2306 7 0.8366783751532978 2306 8 0.7320471966866151 2306 9 1.0096005082454766 2306 10-11 1.206392796224712 2306 12-13 1.3873829962942992 2306 14-15 1.3979095166809472 2306 16-17 1.3901994536803173 2306 18-19 1.312742922538554 2306 20-21 1.5491489946891548 2306 22-23 1.1906988161495775 2306 24-25 1.2292259793433757 2306 26-27 1.336211356861675 2306 28-29 1.6361880836319784 2306 30-31 1.5136845029887667 2306 32-33 1.1784886184362264 2306 34-35 1.2004428661172142 2306 36-37 1.3308498984791584 2306 38-39 1.096084804015021 2306 40-41 1.328741793805591 2306 42-43 1.220372684599468 2306 44-45 1.0093110298303714 2306 46-47 0.9857087910005049 2306 48-49 1.0097756969862743 2306 50-51 1.0291375358398867 2306 52-53 1.1715326399474506 2306 54-55 1.627593778794207 2306 56-57 1.0814345633626985 2306 58-59 1.0650870266509216 2306 60-61 0.8579911865544503 2306 62-63 0.6464524038076931 2306 64-65 0.7702632743555107 2306 66-67 0.8863419471485656 2306 68-69 0.7854766707468777 2306 70-71 0.7251972980044918 2306 72-73 0.5647873822730496 2306 74-75 0.7298117541739799 2306 76-77 0.8097073634299257 2306 78-79 0.8994189948159637 2306 80-81 1.226051566787227 2306 82-83 1.1140277680415487 2306 84-85 1.0150689786441909 2306 86-87 0.8561258744023057 2306 88-89 0.6959553366900373 2306 90-91 0.42967085914050784 2306 92-93 0.2526857078232574 2306 94-95 0.1320375214466587 2306 96-97 0.16987799591811026 2306 98-99 0.385587984631762 2306 100-101 0.3809944198730051 2307 1 0.6897272146839697 2307 2 0.5553370372622588 2307 3 0.3819169102097817 2307 4 0.32917119412498863 2307 5 0.5382480675337789 2307 6 0.6813695126922781 2307 7 0.778557883626533 2307 8 0.8135105996909502 2307 9 1.001915646426248 2307 10-11 1.1164948468436435 2307 12-13 1.347456734682055 2307 14-15 1.3036376754175123 2307 16-17 1.4328193169423713 2307 18-19 1.2472285155193532 2307 20-21 1.4165266742435634 2307 22-23 1.185261605585076 2307 24-25 1.198514091420762 2307 26-27 1.3310289065551402 2307 28-29 1.5540036995020827 2307 30-31 1.5822785271464426 2307 32-33 1.3396444014737838 2307 34-35 1.3498571647922688 2307 36-37 1.3462194468153825 2307 38-39 1.0903333596077687 2307 40-41 1.2484110184453456 2307 42-43 1.2060678573631236 2307 44-45 1.102331711469624 2307 46-47 0.9798082566682638 2307 48-49 1.0409273720469585 2307 50-51 0.9734410219219782 2307 52-53 1.2945599133192403 2307 54-55 1.6239821101912852 2307 56-57 1.2086748842166841 2307 58-59 1.1543963204033005 2307 60-61 1.0108050842782674 2307 62-63 0.885770685950753 2307 64-65 0.9564251717499737 2307 66-67 1.2015351635948122 2307 68-69 0.9801467968091053 2307 70-71 0.8565439995529758 2307 72-73 0.7736980273361347 2307 74-75 1.015357453193058 2307 76-77 0.9800183879503521 2307 78-79 0.9951195399468205 2307 80-81 1.51696986366305 2307 82-83 1.2475728701473408 2307 84-85 1.093887726223869 2307 86-87 1.1060610587130313 2307 88-89 0.8688256629068007 2307 90-91 0.7675358102221423 2307 92-93 0.8965296031522598 2307 94-95 0.8221741752476177 2307 96-97 0.7792940483534743 2307 98-99 0.7951911804908818 2307 100-101 0.6399134000953346 1101 1 1.0796659672836562 1101 2 0.5506822507428204 1101 3 0.33257691530369726 1101 4 0.3309316280362822 1101 5 0.595258717875879 1101 6 0.6078802815213038 1101 7 0.7637517330003902 1101 8 0.631211920859883 1101 9 0.8648969204942603 1101 10-11 0.9196612138407758 1101 12-13 1.159047809545875 1101 14-15 1.3222889708707228 1101 16-17 1.2908073745664694 1101 18-19 1.0200771126364785 1101 20-21 1.3118634736001766 1101 22-23 0.9830229393002128 1101 24-25 1.084792302497732 1101 26-27 1.121924000833097 1101 28-29 1.4535544621541803 1101 30-31 1.3918495685188645 1101 32-33 1.04083002424278 1101 34-35 1.055939906151714 1101 36-37 1.193915349545989 1101 38-39 0.766019434474984 1101 40-41 1.0085793281887092 1101 42-43 1.1115399556055579 1101 44-45 0.9561604294426544 1101 46-47 0.8214328501907247 1101 48-49 0.7614560509181771 1101 50-51 0.830941386016498 1101 52-53 1.0638161206668784 1101 54-55 1.4897346301305205 1101 56-57 1.1538451562458434 1101 58-59 1.0876011079868064 1101 60-61 0.8266743549206481 1101 62-63 0.7129855336759405 1101 64-65 0.708821445709308 1101 66-67 0.7659577975427752 1101 68-69 0.6849803323414356 1101 70-71 0.7756408346078985 1101 72-73 0.6809891592054527 1101 74-75 0.8938333819445887 1101 76-77 0.8181805240290778 1101 78-79 0.7866968617697907 1101 80-81 1.4163633158993392 1101 82-83 1.2099667251407062 1101 84-85 1.0048290618308577 1101 86-87 0.8598316048714665 1101 88-89 0.8044094096063787 1101 90-91 0.630872961593596 1101 92-93 0.6885635388818336 1101 94-95 0.5319876925869664 1101 96-97 0.5953672630463984 1101 98-99 0.7250094346563429 1101 100-101 0.8408415343886908 2201 1 1.4756752755172116 2201 2 0.7990862969334991 2201 3 0.33954317731542005 2201 4 0.2503274334493 2201 5 0.48221140228527304 2201 6 0.5989001752517211 2201 7 0.5545271760726536 2201 8 0.6508880556675152 2201 9 0.600395801685579 2201 10-11 0.7779803679924555 2201 12-13 1.0898386194199503 2201 14-15 1.029120013970612 2201 16-17 1.0054839020050466 2201 18-19 0.872020410286126 2201 20-21 1.13599613631785 2201 22-23 0.9457705156568892 2201 24-25 0.9314259015037578 2201 26-27 1.0104181501394223 2201 28-29 1.4041878580987586 2201 30-31 1.398890292078562 2201 32-33 1.0076013760703475 2201 34-35 0.8813350516718828 2201 36-37 0.928368462400627 2201 38-39 0.7238856626886161 2201 40-41 1.0491984010627604 2201 42-43 0.9891984384689962 2201 44-45 0.9483900509305059 2201 46-47 0.9479601314844857 2201 48-49 1.0081026567715128 2201 50-51 0.9701603866100683 2201 52-53 1.1817961466669331 2201 54-55 1.405754340052063 2201 56-57 1.0097775235959006 2201 58-59 1.1381798368949276 2201 60-61 0.9352976654043204 2201 62-63 0.8351512669850862 2201 64-65 0.7708881446859159 2201 66-67 0.6761620975185707 2201 68-69 0.7666947325965374 2201 70-71 0.8400998062286433 2201 72-73 0.7221705830474434 2201 74-75 0.8444737607318764 2201 76-77 0.8287597770767618 2201 78-79 0.8595743371922708 2201 80-81 1.376298872125112 2201 82-83 1.137435464176125 2201 84-85 1.0299602748459549 2201 86-87 1.057838153925207 2201 88-89 1.0444786703103652 2201 90-91 0.8600219090205385 2201 92-93 0.9659924577119092 2201 94-95 0.8442270582196727 2201 96-97 0.6649261732636056 2201 98-99 0.8237493318946711 2201 100-101 0.850418915314453 2304 1 -1.8042228719552824 2304 2 -0.3039291788984073 2304 3 -0.17198104647679457 2304 4 0.025850678927824333 2304 5 0.3744116066812211 2304 6 0.4159513668110151 2304 7 0.519869719773645 2304 8 0.6527751084688944 2304 9 0.8341302691848398 2304 10-11 0.7921619380452256 2304 12-13 1.1104360671099585 2304 14-15 1.2197151366858137 2304 16-17 1.1871780027599073 2304 18-19 0.8600709761593777 2304 20-21 1.1829817569890864 2304 22-23 0.9849225795863177 2304 24-25 1.0711892004372956 2304 26-27 1.1511720377767674 2304 28-29 1.4947054541601972 2304 30-31 1.5383595001354706 2304 32-33 0.8417117913170529 2304 34-35 0.8402907435323712 2304 36-37 1.0951140716333043 2304 38-39 0.9123831287959945 2304 40-41 0.955221398226989 2304 42-43 0.992753716491741 2304 44-45 0.9976015365971733 2304 46-47 0.9085561386798773 2304 48-49 0.8589820741266152 2304 50-51 0.8621404413148994 2304 52-53 1.0660538353857945 2304 54-55 1.4108403073541425 2304 56-57 1.1496700983094925 2304 58-59 0.807138150659398 2304 60-61 0.6004936512712717 2304 62-63 0.3179236257515967 2304 64-65 0.31560775185056755 2304 66-67 0.5194867052421444 2304 68-69 0.6923057759551874 2304 70-71 0.6370551153804271 2304 72-73 0.44627045100129337 2304 74-75 0.6399293228556715 2304 76-77 0.670889087207442 2304 78-79 0.569201988983238 2304 80-81 1.2887061982022452 2304 82-83 0.9573066701782125 2304 84-85 0.7070470246437317 2304 86-87 0.5780374884933508 2304 88-89 0.38272899140141803 2304 90-91 0.31811149682074813 2304 92-93 0.34779784868416996 2304 94-95 0.26672932109164904 2304 96-97 0.23239146457246918 2304 98-99 0.4442213076031507 2304 100-101 0.5331939449879641 1102 1 1.4565788575262246 1102 2 0.14665589068244955 1102 3 0.3079403858673899 1102 4 0.18437515226971612 1102 5 0.36284853648051296 1102 6 0.26580046824017245 1102 7 -4.719620471682841 1102 8 -1.0622385763481574 1102 9 0.3467567227874113 1102 10-11 0.6952293450452132 1102 12-13 0.995633411120501 1102 14-15 0.8435255997870073 1102 16-17 0.5818188117792289 1102 18-19 0.8767831770359038 1102 20-21 1.19616411536105 1102 22-23 0.7120373012410681 1102 24-25 1.0359623642273164 1102 26-27 1.0974076763579106 1102 28-29 1.030809362736015 1102 30-31 1.3203523161521673 1102 32-33 1.0732558266356662 1102 34-35 1.241642132857777 1102 36-37 1.2414289790261606 1102 38-39 0.9618836848098908 1102 40-41 1.282418185132343 1102 42-43 1.2491639592608337 1102 44-45 1.1636825471128773 1102 46-47 1.017731472942991 1102 48-49 1.0115207086350537 1102 50-51 0.7991208493276076 1102 52-53 0.9375364881886767 1102 54-55 1.3582734083511454 1102 56-57 1.1641392550890473 1102 58-59 1.0712877358588457 1102 60-61 0.9492062799347423 1102 62-63 0.7438683310151291 1102 64-65 0.5633398671915266 1102 66-67 0.6151611053582613 1102 68-69 0.7700693123563589 1102 70-71 0.6868637434720597 1102 72-73 0.6203821928798909 1102 74-75 0.8006278978449757 1102 76-77 0.7357002825663912 1102 78-79 0.8804075242407379 1102 80-81 1.3207497047892929 1102 82-83 1.169379633497666 1102 84-85 0.8604115225833695 1102 86-87 0.7547986175016703 1102 88-89 0.6496922306434492 1102 90-91 0.2789601246286466 1102 92-93 0.38085397989690506 1102 94-95 0.42334343107654604 1102 96-97 0.23522017846157794 1102 98-99 0.2106451069623425 1102 100-101 0.46314982127459814 2202 1 1.5346305595146958 2202 2 0.9292184082468502 2202 3 0.43380369163283916 2202 4 -0.25408495325851277 2202 5 0.1713386078610597 2202 6 0.4657059238851815 2202 7 0.6791527034546547 2202 8 -3.688209644547303 2202 9 -0.3332209585451764 2202 10-11 0.5997721091852739 2202 12-13 0.18704847370605648 2202 14-15 0.22154178549984493 2202 16-17 0.26281801372310554 2202 18-19 0.9011596390299843 2202 20-21 1.2485477121932789 2202 22-23 -2.533188004127254 2202 24-25 -2.926088765757573 2202 26-27 -1.2457219786648537 2202 28-29 0.7070721937034037 2202 30-31 1.3594085073160045 2202 32-33 1.175782336633013 2202 34-35 -1.8500002397405524 2202 36-37 -0.20035659917266457 2202 38-39 0.540190829093703 2202 40-41 1.2219054845180253 2202 42-43 1.3798821161927606 2202 44-45 1.4530338494299286 2202 46-47 0.7371681459174866 2202 48-49 1.030508230125399 2202 50-51 -3.6196224740073575 2202 52-53 -0.2158744925839997 2202 54-55 1.5277136197936088 2202 56-57 1.5037738834139205 2202 58-59 1.4686945505972346 2202 60-61 1.3952101067439813 2202 62-63 1.233630241834618 2202 64-65 1.268117690881656 2202 66-67 1.3523822353343746 2202 68-69 1.3884160348430932 2202 70-71 1.3874753866693297 2202 72-73 1.158074626319408 2202 74-75 0.7706137059564888 2202 76-77 1.4119473951326569 2202 78-79 1.4832803800680558 2202 80-81 1.95951540527874 2202 82-83 1.8229479444991696 2202 84-85 1.666485873106346 2202 86-87 1.7458304289136137 2202 88-89 1.5927270342540893 2202 90-91 1.3115214870733354 2202 92-93 1.343586645132394 2202 94-95 1.3063374370287768 2202 96-97 1.095822027998313 2202 98-99 1.3313922266932785 2202 100-101 1.3374547974425752 2305 1 1.317052815474657 2305 2 0.7160102452060677 2305 3 0.07857192758974207 2305 4 -0.1456395819363152 2305 5 0.29214397088072985 2305 6 0.3505202740776596 2305 7 0.4907501714658764 2305 8 0.04185489421494992 2305 9 0.5227069412953966 2305 10-11 0.3289967717196802 2305 12-13 0.8357659396463717 2305 14-15 0.6037462078851021 2305 16-17 0.7621876220244417 2305 18-19 0.6732672172061314 2305 20-21 0.826904161630388 2305 22-23 0.535489990411115 2305 24-25 0.7399314144618145 2305 26-27 0.9131032590604775 2305 28-29 -2.406520075990521 2305 30-31 -1.056024981396547 2305 32-33 -0.05755311008406494 2305 34-35 -0.6337515343144986 2305 36-37 0.22046945613219293 2305 38-39 -2.8823542970422764 2305 40-41 -4.49174785421371 2305 42-43 -4.519331101711394 2305 44-45 -1.7443404347413782 2305 46-47 -0.36235856061291827 2305 48-49 0.3735116760532122 2305 50-51 0.6106238427220632 2305 52-53 0.25854314885143026 2305 54-55 0.8084749651083172 2305 56-57 0.33812947765529344 2305 58-59 -4.559151572280626 2305 60-61 -0.8886914841317477 2305 62-63 0.2736642858513818 2305 64-65 0.6085168885104117 2305 66-67 0.4686765015388943 2305 68-69 0.1195492312075892 2305 70-71 0.8863964992111519 2305 72-73 0.2581200893496529 2305 74-75 0.763146315602512 2305 76-77 0.15318630719910686 2305 78-79 0.5108383985190095 2305 80-81 0.913300893525328 2305 82-83 1.0684005123143514 2305 84-85 1.029545886935356 2305 86-87 1.1622432535868228 2305 88-89 0.772084132132612 2305 90-91 0.7832486595458761 2305 92-93 0.7190994946066844 2305 94-95 0.5954605014203764 2305 96-97 0.7950168389030488 2305 98-99 0.9434706968002899 2305 100-101 1.0882137954519493 1103 1 1.3416915763692536 1103 2 0.63064373448589 1103 3 0.3046732811788466 1103 4 -0.2501765767883555 1103 5 0.1810776672336516 1103 6 0.2626244066699499 1103 7 0.4904627476973431 1103 8 0.24698549449711749 1103 9 0.14284918199935248 1103 10-11 0.5875298583791491 1103 12-13 0.11497673054065416 1103 14-15 0.18299317963107598 1103 16-17 0.21573094054345177 1103 18-19 0.47468355855134803 1103 20-21 0.5387450265743112 1103 22-23 0.2962129028196614 1103 24-25 0.5805444927318462 1103 26-27 0.668133209415366 1103 28-29 0.4411522938050396 1103 30-31 0.07073739869245799 1103 32-33 -0.04235324734835899 1103 34-35 0.5227261937151795 1103 36-37 0.8714247014921028 1103 38-39 -0.018124783692620383 1103 40-41 -2.9040056590901955 1103 42-43 -0.7908708192103688 1103 44-45 0.17960155575182313 1103 46-47 -2.7450510256443508 1103 48-49 -0.9783411028364384 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1208 34-35 -5.4582530127179965 1208 36-37 -2.552939214589415 1208 38-39 -0.3514784402668951 1208 40-41 -0.748075027298043 1208 42-43 0.1291730463052616 1208 44-45 0.20609679734780428 1208 46-47 -0.06165120807581559 1208 48-49 -1.4983215724114132 1208 50-51 -0.09656209265769178 1208 52-53 -1.5894314137465066 1208 54-55 -0.7177651437018042 1208 56-57 -6.319623132174655 1208 58-59 -2.1410247008318635 1208 60-61 -1.3764323811424006 1208 62-63 0.3224983433820512 1208 64-65 0.028106924596020377 1208 66-67 0.7776494096659334 1208 68-69 0.6150677766611103 1208 70-71 -4.03502164281123 1208 72-73 -0.8443771606229191 1208 74-75 -5.568072802269338 1208 76-77 -1.3588461178046174 1208 78-79 -3.922388508987229 1208 80-81 0.8185029877192775 1208 82-83 0.17061103734686967 1208 84-85 -2.919152034859767 1208 86-87 -0.9652260858345514 1208 88-89 -4.964436191109318 1208 90-91 -0.1630225037086923 1208 92-93 -2.064660696671435 1208 94-95 -4.772642480190775 1208 96-97 -3.40820412193262 1208 98-99 -6.522372315551152 1208 100-101 -4.0288928873257035 1209 1 1.7337433006481149 1209 2 1.1027503872024802 1209 3 0.5258322753018305 1209 4 0.47841911067537524 1209 5 0.4717774326434494 1209 6 1.137177908766546 1209 7 0.8555289356474134 1209 8 0.34194854858364465 1209 9 0.1652435551040341 1209 10-11 -2.7933852171065823 1209 12-13 -0.19919891376500942 1209 14-15 -1.3232227966156458 1209 16-17 -0.40803812501920333 1209 18-19 -16.93394274007299 1209 20-21 -14.047784887763484 1209 22-23 -3.564038617521021 1209 24-25 -2.0907684256683723 1209 26-27 -1.5243449356872532 1209 28-29 -3.8200282895644015 1209 30-31 -0.8791899559964875 1209 32-33 -0.671543124413283 1209 34-35 -3.378580655583093 1209 36-37 -3.736303645913914 1209 38-39 -1.5735737950624795 1209 40-41 -10.911454357809351 1209 42-43 -5.1354754999796945 1209 44-45 -2.8065779547965626 1209 46-47 -3.2986422685637287 1209 48-49 -3.055603375909243 1209 50-51 -2.6200979342528754 1209 52-53 -1.06100808189845 1209 54-55 -2.670696941629842 1209 56-57 -0.9832600654485759 1209 58-59 -1.4879494808251792 1209 60-61 -1.3929433257786954 1209 62-63 -0.554562478718875 1209 64-65 -0.23150444334427434 1209 66-67 0.34136431999232286 1209 68-69 -1.0799270712836382 1209 70-71 -1.1795855282963643 1209 72-73 -0.39786802616820083 1209 74-75 0.3995179852988926 1209 76-77 -16.531231101652228 1209 78-79 -16.629358404275187 1209 80-81 -22.051947190513985 1209 82-83 -10.161905393532042 1209 84-85 -3.2388892800418994 1209 86-87 -2.0058959922620865 1209 88-89 -1.3044927244723574 1209 90-91 -5.930838867750133 1209 92-93 -3.48388134388097 1209 94-95 -0.5656203681266163 1209 96-97 0.389354917835675 1209 98-99 1.6932725268238045 1209 100-101 0.8389011975578882 1212 1 -28.62795882701146 1212 2 -16.24094846713467 1212 3 -5.689695547938758 1212 4 -1.2406889089645574 1212 5 -0.41774793560531975 1212 6 -2.6758335478619273 1212 7 -0.3759563344016854 1212 8 -1.7384524334130802 1212 9 -0.09457641216274482 1212 10-11 0.5936340136626512 1212 12-13 0.36002776381273094 1212 14-15 0.047941278670769805 1212 16-17 -0.1552251135625795 1212 18-19 -0.9605384847538403 1212 20-21 -0.6167803869451518 1212 22-23 -0.5923221690758496 1212 24-25 0.26996193439709515 1212 26-27 -0.6008077016447047 1212 28-29 0.1953665549527841 1212 30-31 -0.38133807383609053 1212 32-33 0.15829525529211708 1212 34-35 -0.8970442398711427 1212 36-37 -1.3776191123623533 1212 38-39 -0.3598872156844095 1212 40-41 -0.2993942105098455 1212 42-43 0.30719021196792795 1212 44-45 1.1812493774456598 1212 46-47 -1.8569994698730596 1212 48-49 -0.9514196606882948 1212 50-51 0.3195501835867347 1212 52-53 -0.9715031719148186 1212 54-55 -0.21355700709629133 1212 56-57 -1.3653181669215755 1212 58-59 -0.16348139571879727 1212 60-61 0.5371916987712169 1212 62-63 -4.258173362515926 1212 64-65 -3.228537994899103 1212 66-67 -1.2715141579127476 1212 68-69 0.3637615539209449 1212 70-71 -0.5163285724862128 1212 72-73 -0.9685513322238499 1212 74-75 0.11279539937417837 1212 76-77 1.15606431569638 1212 78-79 -1.1218399918365591 1212 80-81 0.6345298962290578 1212 82-83 0.5050340500031432 1212 84-85 -3.416518167112276 1212 86-87 -1.8557834718038215 1212 88-89 -0.8445397784821793 1212 90-91 0.08118036301910081 1212 92-93 -2.155117023095375 1212 94-95 -1.727496391039871 1212 96-97 0.1925975528602244 1212 98-99 -0.5571366384789798 1212 100-101 0.056372555986691 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 5.0 10 10.0 11 16.0 12 61.0 13 252.0 14 864.0 15 2026.0 16 3824.0 17 6418.0 18 9891.0 19 14488.0 20 20658.0 21 27893.0 22 37204.0 23 47808.0 24 60868.0 25 76157.0 26 94380.0 27 113869.0 28 136784.0 29 164117.0 30 192811.0 31 225610.0 32 262818.0 33 300435.0 34 339062.0 35 374588.0 36 399696.0 37 360841.0 38 122308.0 39 273.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.30288061278755 25.50263093970861 11.204561383007341 17.989927064496506 2 22.418135500936852 26.652329968636142 32.30312487209017 18.626409658336833 3 18.94601017835831 26.3571365262193 33.19479898434318 21.502054311079206 4 21.824049083078574 26.203564271071173 30.938611405408302 21.03377524044195 5 19.657288876037313 29.374170724826993 29.559150987120873 21.409389412014825 6 19.789383982158032 28.835984756354314 29.62378512843496 21.750846133052693 7 21.97104390469129 28.538903274933908 29.963887329882645 19.526165490492158 8 19.57324976928998 27.65010285515062 30.874242278796643 21.902405096762756 9 21.186600385508054 28.139761687001148 29.202500089810542 21.471137837680264 10-11 21.414601367829217 28.568849936308133 28.508220461608495 21.50832823425415 12-13 21.299975498211634 28.45076564032053 28.608249741816643 21.641009119651187 14-15 21.057214318567695 28.79238983780511 28.653183503835937 21.49721233979125 16-17 21.370915736117137 28.748856750633532 28.475949213520995 21.40427829972833 18-19 21.710094313467028 28.737801427753805 28.009641973774645 21.542462285004515 20-21 21.28836916553483 28.697445184277264 28.351969022721335 21.66221662746657 22-23 21.21881439489193 28.451934283420854 28.62507722233968 21.704174099347533 24-25 21.40170398564445 28.334635831518963 28.69002054747326 21.573639635363325 26-27 21.38274071138191 28.5167892703203 28.57534279651759 21.525127221780206 28-29 21.638494622262765 28.472487645897953 28.41733514171514 21.47168259012414 30-31 21.65582363919367 28.320030576807444 28.30407080250574 21.72007498149314 32-33 21.31748820242883 28.41368382803726 28.540640593538708 21.728187375995205 34-35 21.624375227839028 28.401360644374414 28.482249020770674 21.49201510701588 36-37 21.67855601144628 28.404614435998653 28.38363410538987 21.533195447165195 38-39 21.473213786182605 28.326361483587643 28.60805738922827 21.592367341001484 40-41 21.700457409206106 28.340201023299795 28.355837183111948 21.603504384382155 42-43 21.471358683265617 28.338846620679746 28.54990138508462 21.639893310970017 44-45 21.3235540945066 28.31314019454435 28.75522888730927 21.60807682363978 46-47 21.64606040328153 28.398192649369147 28.500129298436693 21.455617648912625 48-49 21.672879300196083 28.306258147364115 28.417078494727825 21.603784057711977 50-51 21.51410078015969 28.372112128980987 28.494431227235406 21.619355863623923 52-53 21.559295342743916 28.39575516617325 28.50703202618113 21.537917464901707 54-55 21.52299320621307 28.273037118549766 28.61089341029317 21.593076264943996 56-57 21.454584216788337 28.405920563181127 28.566065109940546 21.57343011008999 58-59 21.628868987531114 28.307113941249895 28.55759694297853 21.506420128240457 60-61 21.695472742076305 28.22254809723836 28.426141458221988 21.65583770246335 62-63 21.454312881156646 28.328423874531044 28.583282869485544 21.633980374826763 64-65 21.58688409892963 28.306055163334065 28.565377280331877 21.54168345740443 66-67 21.663689795661153 28.21746848813578 28.538487621924475 21.580354094278594 68-69 21.537772697129355 28.306612813363913 28.54818410008328 21.607430389423456 70-71 21.767635772394115 28.282917367443954 28.333713445044463 21.61573341511747 72-73 21.610675386076927 28.284102541442973 28.504429666615117 21.600792405864983 74-75 21.437701666416757 28.21630040574112 28.7276821025833 21.618315825258822 76-77 21.587737407101567 28.454214344697416 28.508051197357553 21.449997050843457 78-79 21.766454551250085 28.317916059891722 28.338469479864493 21.577159908993696 80-81 21.537993271353137 28.253978782284424 28.548417883608412 21.659610062754027 82-83 21.61776123228944 28.3567888893944 28.434632977712326 21.590816900603834 84-85 21.574778780150634 28.202738813561645 28.535170418681545 21.68731198760618 86-87 21.435162492603062 28.38643495709372 28.584206843007376 21.594195707295842 88-89 21.638109283359398 28.337029294955997 28.426816058019504 21.598045363665104 90-91 21.74059407981834 28.19873444004789 28.25909667234603 21.801574807787745 92-93 21.447959336928065 28.285763507484447 28.508529880349027 21.75774727523846 94-95 21.62605665233574 28.33990635741476 28.330173088734277 21.703863901515223 96-97 21.674065191437933 28.335093391037315 28.28723906138371 21.70360235614105 98-99 21.505486541254122 28.309189371296945 28.410170826225023 21.775153261223917 100-101 21.72755676802558 28.204966987808923 28.230705880291474 21.836770363874024 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5.0 1 8.5 2 12.0 3 12.0 4 19.0 5 46.5 6 89.0 7 125.0 8 197.0 9 317.5 10 451.5 11 684.0 12 1017.5 13 1404.0 14 1958.5 15 2722.5 16 3700.0 17 5175.0 18 7083.0 19 9459.0 20 12694.0 21 16824.5 22 21460.5 23 26917.0 24 33607.0 25 41085.5 26 49428.5 27 58023.5 28 66796.0 29 76099.0 30 84877.5 31 92315.0 32 98770.0 33 104149.0 34 107910.0 35 110228.5 36 110890.5 37 110113.5 38 108325.0 39 105347.0 40 101666.0 41 97884.0 42 94098.0 43 89951.0 44 86636.0 45 84185.0 46 81410.5 47 79480.5 48 77699.0 49 75028.5 50 72249.0 51 69716.5 52 66761.5 53 63663.5 54 60863.5 55 57631.5 56 54172.5 57 50956.0 58 47844.0 59 44892.5 60 41915.5 61 39147.0 62 36857.0 63 34890.5 64 33178.0 65 31673.5 66 30252.5 67 28769.0 68 27351.5 69 25807.5 70 24015.0 71 22073.0 72 19696.5 73 16927.0 74 14207.5 75 11608.5 76 9104.5 77 6819.0 78 4897.0 79 3311.0 80 2211.0 81 1442.0 82 889.5 83 590.5 84 395.0 85 265.5 86 179.0 87 128.0 88 96.0 89 68.5 90 49.5 91 30.5 92 22.5 93 15.5 94 9.0 95 5.5 96 3.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7193382406203935 2 0.0026795931023151096 3 2.650147024267691E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.1910903234887242 14-15 0.0 16-17 4.711372487587006E-4 18-19 0.11149757452655124 20-21 1.4723039023709394E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0018698259560110929 42-43 0.0 44-45 0.0 46-47 0.023321293813555678 48-49 2.9446078047418787E-5 50-51 7.361519511854696E-5 52-53 1.1778431218967515E-4 54-55 0.001987610268200768 56-57 0.0012809043950627172 58-59 0.09402132720540818 60-61 0.0015606421365131958 62-63 0.0012514583170152983 64-65 0.002017056346248187 66-67 0.003960497497377827 68-69 0.004299127394923143 70-71 0.0031360073120501007 72-73 0.038662700476260865 74-75 0.044434131773554944 76-77 0.1542385568123796 78-79 2.797377414504785E-4 80-81 0.11264597157040056 82-83 0.004343296511994271 84-85 0.004461080824183946 86-87 0.00608061511679198 88-89 0.0063161837411713296 90-91 0.02070059286733541 92-93 0.009658313599553362 94-95 0.013766041487168283 96-97 0.009304960662984335 98-99 0.010747818487307857 100-101 0.011734262101896386 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3396038.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.9494571454674 #Duplication Level Percentage of deduplicated Percentage of total 1 98.70273748172211 96.67879555106259 2 0.9581688317612875 1.8770423384944959 3 0.14496065200147548 0.42596451512992606 4 0.0800357432993207 0.3135783043360977 5 0.046154894085440226 0.2260423410137707 6 0.03208975179739823 0.18859042610927662 7 0.009179292390601888 0.06293746946472821 8 0.014474396020707866 0.11342073861894791 9 0.007156256703828699 0.06308563134002697 >10 0.0050427002178391 0.050542684430149094 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.061225463319315E-4 0.0 5.8892156094837574E-5 2 2.061225463319315E-4 0.0 8.833823414225636E-5 3 2.355686243793503E-4 2.9446078047418787E-5 8.833823414225636E-5 4 2.355686243793503E-4 2.9446078047418787E-5 8.833823414225636E-5 5 2.355686243793503E-4 2.9446078047418787E-5 8.833823414225636E-5 6 2.355686243793503E-4 2.9446078047418787E-5 8.833823414225636E-5 7 2.9446078047418785E-4 5.8892156094837574E-5 8.833823414225636E-5 8 2.9446078047418785E-4 5.8892156094837574E-5 8.833823414225636E-5 9 2.9446078047418785E-4 8.833823414225636E-5 8.833823414225636E-5 10-11 3.2390685852160667E-4 1.1778431218967515E-4 1.4723039023709393E-4 12-13 3.5335293656902543E-4 1.1778431218967515E-4 1.4723039023709393E-4 14-15 3.827990146164442E-4 1.1778431218967515E-4 1.4723039023709393E-4 16-17 4.564142097349912E-4 1.1778431218967515E-4 2.061225463319315E-4 18-19 5.005833268061194E-4 1.3250735121338454E-4 2.061225463319315E-4 20-21 5.005833268061194E-4 1.7667646828451272E-4 2.061225463319315E-4 22-23 5.005833268061194E-4 2.061225463319315E-4 2.355686243793503E-4 24-25 5.447524438772476E-4 2.355686243793503E-4 2.650147024267691E-4 26-27 7.214289121617603E-4 2.355686243793503E-4 2.650147024267691E-4 28-29 7.950441072803072E-4 2.650147024267691E-4 2.650147024267691E-4 30-31 0.0010453357706833668 3.2390685852160667E-4 2.650147024267691E-4 32-33 0.0013545195901812643 3.5335293656902543E-4 2.650147024267691E-4 34-35 0.0018256568389399647 3.680759755927348E-4 2.650147024267691E-4 36-37 0.002208455853556409 3.827990146164442E-4 2.650147024267691E-4 38-39 0.0027826543754810753 3.827990146164442E-4 2.650147024267691E-4 40-41 0.003857436224211861 3.827990146164442E-4 2.9446078047418785E-4 42-43 0.0050941715022034505 4.12245092663863E-4 2.9446078047418785E-4 44-45 0.006846213146024868 4.12245092663863E-4 2.9446078047418785E-4 46-47 0.00945219105322143 4.12245092663863E-4 3.0918381949789726E-4 48-49 0.013280181199385873 4.12245092663863E-4 3.2390685852160667E-4 50-51 0.01775598506259353 4.12245092663863E-4 3.386298975453161E-4 52-53 0.023910215374504055 4.12245092663863E-4 3.5335293656902543E-4 54-55 0.030682813325410375 4.12245092663863E-4 3.680759755927348E-4 56-57 0.040193896534726645 4.12245092663863E-4 4.12245092663863E-4 58-59 0.051545359622006584 4.12245092663863E-4 4.12245092663863E-4 60-61 0.0653702932652697 4.12245092663863E-4 4.12245092663863E-4 62-63 0.08208094255717986 4.564142097349912E-4 4.12245092663863E-4 64-65 0.10228095209770915 4.711372487587006E-4 4.269681316875724E-4 66-67 0.12667702775999562 5.153063658298288E-4 4.416911707112818E-4 68-69 0.1566973043293391 5.300294048535382E-4 4.416911707112818E-4 70-71 0.19213565925940757 5.300294048535382E-4 4.416911707112818E-4 72-73 0.2315197886478302 5.741985219246663E-4 4.416911707112818E-4 74-75 0.2730829278117618 6.183676389957945E-4 4.416911707112818E-4 76-77 0.32375962813136955 6.478137170432133E-4 4.416911707112818E-4 78-79 0.3839179655822461 6.478137170432133E-4 4.416911707112818E-4 80-81 0.4518795137156887 6.478137170432133E-4 4.416911707112818E-4 82-83 0.5269670127366066 7.067058731380509E-4 4.416911707112818E-4 84-85 0.609018509215739 7.214289121617603E-4 4.711372487587006E-4 86-87 0.7016117016358474 7.361519511854697E-4 4.711372487587006E-4 88-89 0.8069697688895119 7.361519511854697E-4 5.005833268061194E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCTC 1320 4.2466127E-8 7.242107 1 GTCTAAG 755 0.0019315762 6.9639335 1 GTGCGAA 900 0.0017255347 6.373054 1 GTTAAGT 1340 2.0752712E-5 6.0639133 1 GTTTAAT 2875 1.8189894E-12 5.818876 1 GGTGACT 1095 0.0024540597 5.674637 1 GTACTTT 2640 1.6734703E-10 5.612633 1 GTATACA 1545 3.0301288E-5 5.568688 1 CACGTCT 2650 0.0 5.551808 94-95 GTTTATT 4760 0.0 5.4224515 1 GTTCAAA 3465 0.0 5.3798513 1 GGTTATT 2140 2.7200622E-7 5.360513 1 GTTTTAG 2690 1.5752448E-9 5.3306217 1 GTAAAAT 5420 0.0 5.2912817 1 GTTAATC 1450 4.1480418E-4 5.2742515 1 GTTATCT 1725 3.081825E-5 5.264697 1 GTATCAA 1910 5.5599303E-6 5.255267 1 GTCTTTA 2110 1.1587017E-6 5.2101984 1 GTTATTC 1840 1.6204816E-5 5.1954246 1 GTTTGAG 2585 1.8262654E-8 5.177336 1 >>END_MODULE