##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib36_CCAACA_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3168766 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.752057109928597 27.0 22.0 33.0 2.0 33.0 2 24.759236560856813 27.0 15.0 33.0 2.0 33.0 3 25.67231849874683 33.0 22.0 33.0 6.0 33.0 4 27.75626979082709 33.0 22.0 37.0 2.0 37.0 5 27.819244778566798 33.0 15.0 37.0 6.0 37.0 6 28.86189608194483 33.0 22.0 37.0 15.0 37.0 7 28.540011158918013 33.0 27.0 37.0 6.0 37.0 8 28.302158947678688 33.0 22.0 37.0 6.0 37.0 9 29.025160583015598 33.0 27.0 37.0 15.0 37.0 10-11 28.624549903653346 33.0 24.5 37.0 10.5 37.0 12-13 28.90672125994788 33.0 27.0 37.0 10.5 37.0 14-15 29.205242987333243 33.0 24.5 37.0 6.0 40.0 16-17 30.00190673593443 33.0 27.0 37.0 15.0 40.0 18-19 30.275463066695362 33.0 27.0 37.0 15.0 40.0 20-21 30.25854512450588 33.0 27.0 37.0 15.0 40.0 22-23 30.23872242380788 33.0 27.0 37.0 15.0 40.0 24-25 29.816541991425055 33.0 27.0 37.0 10.5 40.0 26-27 29.279587700701157 33.0 24.5 37.0 6.0 40.0 28-29 29.419319539530527 33.0 22.0 37.0 15.0 40.0 30-31 29.30471530557952 33.0 24.5 37.0 10.5 40.0 32-33 29.10884662988684 33.0 22.0 37.0 6.0 40.0 34-35 29.115600836413922 33.0 22.0 37.0 10.5 40.0 36-37 29.356578081183653 33.0 27.0 37.0 6.0 40.0 38-39 29.339912445412505 33.0 24.5 37.0 15.0 40.0 40-41 29.37532670446477 33.0 24.5 37.0 15.0 40.0 42-43 29.228653362223653 33.0 22.0 37.0 15.0 40.0 44-45 28.945153570822207 33.0 22.0 37.0 10.5 40.0 46-47 28.552059855476863 33.0 22.0 37.0 6.0 40.0 48-49 28.355344004574654 33.0 22.0 37.0 10.5 40.0 50-51 27.55102538338268 30.0 22.0 37.0 10.5 38.5 52-53 27.285978674348314 33.0 22.0 37.0 6.0 37.0 54-55 28.186326790933755 33.0 22.0 37.0 6.0 40.0 56-57 28.057816197219992 33.0 22.0 37.0 6.0 40.0 58-59 28.207628300732843 33.0 22.0 37.0 10.5 40.0 60-61 27.952558661636736 33.0 22.0 37.0 6.0 40.0 62-63 27.771960914753567 33.0 22.0 37.0 6.0 40.0 64-65 27.718787849907503 33.0 22.0 37.0 6.0 37.0 66-67 27.578558183217062 33.0 22.0 37.0 6.0 37.0 68-69 27.265287338983065 33.0 22.0 37.0 6.0 37.0 70-71 27.1619488469644 27.0 22.0 37.0 6.0 37.0 72-73 26.931903144631065 27.0 22.0 33.0 6.0 37.0 74-75 26.8640254597531 27.0 22.0 33.0 6.0 37.0 76-77 26.48635509848313 27.0 22.0 33.0 6.0 37.0 78-79 25.95469545558113 27.0 22.0 33.0 6.0 37.0 80-81 25.842954796914633 27.0 22.0 33.0 6.0 37.0 82-83 25.44573344955102 27.0 18.5 33.0 6.0 37.0 84-85 25.548784290162164 27.0 22.0 33.0 6.0 37.0 86-87 25.262849954840465 27.0 22.0 33.0 6.0 37.0 88-89 24.915812338304562 27.0 15.0 33.0 6.0 37.0 90-91 24.796803550656627 27.0 15.0 33.0 6.0 37.0 92-93 24.69598149563584 27.0 15.0 33.0 6.0 37.0 94-95 24.504754847786174 27.0 15.0 33.0 6.0 37.0 96-97 24.40339646411253 27.0 15.0 33.0 6.0 37.0 98-99 24.174401801837057 27.0 15.0 33.0 6.0 37.0 100-101 23.706980414457867 27.0 15.0 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.309992306297211 2306 2 8.425062719765073 2306 3 7.441252733447811 2306 4 9.039129741886484 2306 5 8.811704382668356 2306 6 7.814544247170694 2306 7 8.206065645608678 2306 8 8.581994717650744 2306 9 7.475694264526528 2306 10-11 7.822933837492485 2306 12-13 7.389669800134833 2306 14-15 7.944291317023055 2306 16-17 6.570421211385732 2306 18-19 6.091674994703993 2306 20-21 5.99221785180821 2306 22-23 5.680646768285754 2306 24-25 5.7589634646119485 2306 26-27 6.222356441504207 2306 28-29 5.300522093210031 2306 30-31 5.222252514140706 2306 32-33 5.080962631329559 2306 34-35 4.506233822744541 2306 36-37 4.68879821029525 2306 38-39 4.3635007809462465 2306 40-41 3.6225602886760058 2306 42-43 3.3023287942629267 2306 44-45 3.025947116473546 2306 46-47 3.4246156897455826 2306 48-49 3.14479776590888 2306 50-51 2.9087154604747916 2306 52-53 3.459281986001283 2306 54-55 2.96251252996165 2306 56-57 3.230283002815227 2306 58-59 2.921908247834054 2306 60-61 2.8989941657911515 2306 62-63 2.3218379715508384 2306 64-65 2.0652112002995544 2306 66-67 1.9727549381240017 2306 68-69 1.9718652886034214 2306 70-71 2.1434399173890384 2306 72-73 1.3598809477651912 2306 74-75 1.0927635849164865 2306 76-77 1.45749360519822 2306 78-79 1.7360033420753034 2306 80-81 1.3976370156880122 2306 82-83 1.4578785356281294 2306 84-85 0.9971780917433364 2306 86-87 0.9297903993663788 2306 88-89 0.8596782256843696 2306 90-91 0.8209510881569742 2306 92-93 0.7869380968705215 2306 94-95 1.0483721056595563 2306 96-97 0.7760109165846707 2306 98-99 0.4728483834768049 2306 100-101 0.3639647802084873 2307 1 8.327313066885381 2307 2 8.415828750757331 2307 3 7.526011112757814 2307 4 9.148700250059761 2307 5 9.052937779088968 2307 6 7.52640193483095 2307 7 8.104091431940933 2307 8 8.59280093751262 2307 9 7.556001792530839 2307 10-11 7.840718322741083 2307 12-13 7.390810347057702 2307 14-15 8.166469124436222 2307 16-17 6.763244723963762 2307 18-19 6.403775913705935 2307 20-21 6.495085464852853 2307 22-23 6.003110664938667 2307 24-25 6.182382330183916 2307 26-27 6.76519465837767 2307 28-29 6.054018527470976 2307 30-31 5.850995827021578 2307 32-33 5.868728787740512 2307 34-35 5.596894312532093 2307 36-37 5.559399939791216 2307 38-39 5.137860007294179 2307 40-41 4.455656159139753 2307 42-43 4.087797540979597 2307 44-45 4.089079775019172 2307 46-47 4.479738418479808 2307 48-49 4.254136266521861 2307 50-51 4.027454443896666 2307 52-53 4.57745890897327 2307 54-55 4.1735216833229565 2307 56-57 4.587537076153453 2307 58-59 4.138601736660661 2307 60-61 4.102119989909269 2307 62-63 3.766370266284092 2307 64-65 3.6409989778440064 2307 66-67 3.304257263998796 2307 68-69 3.359007642749397 2307 70-71 3.058052150966205 2307 72-73 2.4231127333538254 2307 74-75 2.064779991628985 2307 76-77 2.351011720207101 2307 78-79 2.6226151857547713 2307 80-81 2.4578886799897006 2307 82-83 2.6138589892215407 2307 84-85 1.9303780931776053 2307 86-87 1.8607638633864916 2307 88-89 2.044316831241609 2307 90-91 1.8138726741087368 2307 92-93 1.6459291820871869 2307 94-95 1.8096348738126302 2307 96-97 1.6365689342297074 2307 98-99 1.5770695569130346 2307 100-101 1.1379884349237095 1101 1 7.996181261883422 1101 2 7.6968588649437315 1101 3 7.133210464798207 1101 4 8.640333924711364 1101 5 8.442162075955185 1101 6 7.242886396245503 1101 7 6.903823969751205 1101 8 7.9543498390456975 1101 9 7.308894432481747 1101 10-11 7.509814727798613 1101 12-13 7.166392839294126 1101 14-15 7.440227794921174 1101 16-17 6.699092305005291 1101 18-19 5.818544216885861 1101 20-21 5.607709008588703 1101 22-23 5.106768062614542 1101 24-25 5.737507130933825 1101 26-27 6.359028046694828 1101 28-29 5.777722532869372 1101 30-31 5.597415211188164 1101 32-33 5.653963729067403 1101 34-35 5.378380309872632 1101 36-37 5.252810443509272 1101 38-39 4.840385871341866 1101 40-41 4.284262091833018 1101 42-43 4.1122545342465955 1101 44-45 4.161247798671731 1101 46-47 4.4265240766939975 1101 48-49 4.184465015813842 1101 50-51 3.552897727996797 1101 52-53 4.519918974587895 1101 54-55 4.245433068757929 1101 56-57 4.528541202893514 1101 58-59 3.9878948596155723 1101 60-61 4.224847663107177 1101 62-63 3.8264453149692486 1101 64-65 3.5342538567582267 1101 66-67 3.196712509321305 1101 68-69 3.3543120297397984 1101 70-71 3.213743590065448 1101 72-73 2.8668207775539614 1101 74-75 2.3620808531560407 1101 76-77 2.7030809823938604 1101 78-79 3.023177237185184 1101 80-81 2.664060989695656 1101 82-83 2.810762975900513 1101 84-85 2.2050207110613194 1101 86-87 2.1050195373804286 1101 88-89 2.271083147155956 1101 90-91 2.1530103668063134 1101 92-93 1.9727623253418507 1101 94-95 2.272064269647462 1101 96-97 2.1227452905853497 1101 98-99 2.014418801450997 1101 100-101 1.6423308696321932 2201 1 7.0116334025507285 2201 2 7.721449068908889 2201 3 6.155615254105893 2201 4 8.304916023336737 2201 5 3.9981437573483944 2201 6 5.778942422617412 2201 7 6.681000150543905 2201 8 7.743934490061605 2201 9 6.571622398846859 2201 10-11 3.989488100119708 2201 12-13 6.391094807440879 2201 14-15 6.994877402118281 2201 16-17 5.932580727551322 2201 18-19 1.9607884146233197 2201 20-21 4.925519457058758 2201 22-23 5.037909965718171 2201 24-25 5.3752215459471735 2201 26-27 6.1009358530438895 2201 28-29 4.953572925188109 2201 30-31 5.127126426251088 2201 32-33 5.194152501976131 2201 34-35 4.760378338437764 2201 36-37 4.786096815663679 2201 38-39 4.222381363304457 2201 40-41 3.966544213567797 2201 42-43 3.8831097330587667 2201 44-45 3.602780383615393 2201 46-47 4.142887863895066 2201 48-49 3.8114337614603073 2201 50-51 3.4963810760747016 2201 52-53 4.373196431373767 2201 54-55 3.8917429351566533 2201 56-57 4.043332690164846 2201 58-59 3.5998139844746433 2201 60-61 3.557512477120742 2201 62-63 3.317602213288282 2201 64-65 3.189021113896846 2201 66-67 2.838914753126236 2201 68-69 3.1747741435704775 2201 70-71 3.0020037872182 2201 72-73 2.3674605381862506 2201 74-75 2.000817087456099 2201 76-77 2.37786401235865 2201 78-79 2.8227585125198935 2201 80-81 2.3130483190186197 2201 82-83 2.525423681838898 2201 84-85 2.0389554788219897 2201 86-87 2.0349482287465435 2201 88-89 2.1194030195149587 2201 90-91 1.8687096693942848 2201 92-93 1.7636575157455532 2201 94-95 1.9593398882069373 2201 96-97 1.8163862869325165 2201 98-99 1.6928935985470055 2201 100-101 1.2615688927527877 2304 1 1.3328134032601966 2304 2 4.372686240775639 2304 3 5.56973846159212 2304 4 4.31101148590194 2304 5 6.4534379147215795 2304 6 6.177711485437598 2304 7 7.007399757952644 2304 8 6.3828791049336715 2304 9 5.635347504165733 2304 10-11 6.598259513434755 2304 12-13 6.475918858411035 2304 14-15 6.663406260104988 2304 16-17 5.50134895293375 2304 18-19 5.151144456114771 2304 20-21 4.79914291702848 2304 22-23 4.459664328189582 2304 24-25 4.5669099473296875 2304 26-27 5.6835828548117 2304 28-29 4.91341558321059 2304 30-31 4.930376273672572 2304 32-33 4.9284734339378495 2304 34-35 4.66669236317545 2304 36-37 4.703665585526508 2304 38-39 4.342355549966776 2304 40-41 3.864105335034278 2304 42-43 3.3642310569842877 2304 44-45 3.080142305527062 2304 46-47 4.111181267849471 2304 48-49 3.751969695204366 2304 50-51 3.3364579436689112 2304 52-53 3.9652023298196752 2304 54-55 3.783729247521375 2304 56-57 4.3240886512534615 2304 58-59 3.6273882393758896 2304 60-61 3.6427738230607183 2304 62-63 3.3884628084128856 2304 64-65 3.2070241955466976 2304 66-67 3.1305805769887236 2304 68-69 3.2323838617452907 2304 70-71 3.239699059443126 2304 72-73 2.8778315291662793 2304 74-75 2.2534143622635696 2304 76-77 2.5305915509804784 2304 78-79 2.9061081584141704 2304 80-81 2.6501546080330556 2304 82-83 2.883840216113068 2304 84-85 2.4180871354029385 2304 86-87 2.098709550565122 2304 88-89 2.351230654625134 2304 90-91 2.318917486329454 2304 92-93 2.21780056084701 2304 94-95 2.416138791606553 2304 96-97 2.362839754550908 2304 98-99 2.1928470895162278 2304 100-101 1.8956610057871224 1102 1 7.0525046143077645 1102 2 7.453588881487409 1102 3 6.022694727232448 1102 4 6.049793847946187 1102 5 6.513785968001436 1102 6 5.861871696509539 1102 7 6.761735978453206 1102 8 6.242356451557235 1102 9 4.730485240037957 1102 10-11 6.020083316261413 1102 12-13 5.70888967364386 1102 14-15 6.027514253585782 1102 16-17 5.209542669831041 1102 18-19 5.25498816773564 1102 20-21 5.517051379135555 1102 22-23 5.204010668953359 1102 24-25 1.6287259093833093 1102 26-27 5.346407284015083 1102 28-29 4.8612987988886935 1102 30-31 4.792834504056415 1102 32-33 4.880228837722701 1102 34-35 4.7946534019073965 1102 36-37 4.765966570357275 1102 38-39 3.8982583636505197 1102 40-41 3.7544157003691723 1102 42-43 3.869880368546461 1102 44-45 -3.8291538848944526 1102 46-47 1.1195461148072958 1102 48-49 -0.8441659175692848 1102 50-51 2.4214916071325128 1102 52-53 -0.5251784179690446 1102 54-55 2.2534958338355473 1102 56-57 -0.9109576874720453 1102 58-59 2.664515568131918 1102 60-61 3.740579316663215 1102 62-63 3.466482582818781 1102 64-65 3.0040908525892682 1102 66-67 3.0219772686799153 1102 68-69 3.299325264012598 1102 70-71 3.1176507639214677 1102 72-73 2.4697148050573325 1102 74-75 2.1885556828631714 1102 76-77 2.640275949737049 1102 78-79 -1.4471412931711605 1102 80-81 1.3533491386777996 1102 82-83 2.5108169819559443 1102 84-85 2.098426956463271 1102 86-87 2.183983380166591 1102 88-89 2.217655724628923 1102 90-91 1.940971469668419 1102 92-93 1.9238785967031369 1102 94-95 2.3647517152873867 1102 96-97 2.2878095280872657 1102 98-99 2.2825592242516173 1102 100-101 1.7211607342990334 2202 1 -20.4192122232708 2202 2 -7.465589559936722 2202 3 -0.7120915982892235 2202 4 1.4373847113310028 2202 5 0.9300095901747802 2202 6 1.4734528239014253 2202 7 3.9143437857960386 2202 8 2.547754062297585 2202 9 3.0980062031178583 2202 10-11 1.7943755555201406 2202 12-13 1.1280936548084064 2202 14-15 2.507379233717291 2202 16-17 1.508995008373649 2202 18-19 1.5620681157266993 2202 20-21 1.141661584775182 2202 22-23 1.412514382953244 2202 24-25 2.476913373652234 2202 26-27 3.498605930490136 2202 28-29 -1.1269683493703084 2202 30-31 0.8667499000419596 2202 32-33 1.4501821528385896 2202 34-35 1.4313792336070108 2202 36-37 3.0335303412938437 2202 38-39 2.447934462836578 2202 40-41 1.6959484939716916 2202 42-43 0.5283417387406644 2202 44-45 0.5836895661843045 2202 46-47 -1.3849364991668445 2202 48-49 0.677640549594301 2202 50-51 1.790237512390643 2202 52-53 1.5672743075988578 2202 54-55 -0.7852510686241168 2202 56-57 1.0253036393520176 2202 58-59 1.9269783348147413 2202 60-61 2.0269049060807944 2202 62-63 -1.9374791186807272 2202 64-65 -0.12164281371363117 2202 66-67 0.5107877122523625 2202 68-69 1.447945721724043 2202 70-71 -2.4468559644452412 2202 72-73 -4.493685832379359 2202 74-75 -1.286354113415733 2202 76-77 -0.08886375596248897 2202 78-79 1.622787904842376 2202 80-81 1.550248055298269 2202 82-83 -0.7166269391759812 2202 84-85 -0.26419625330762386 2202 86-87 0.6047274178396727 2202 88-89 1.139860608355697 2202 90-91 1.3364228148031927 2202 92-93 1.2646125045157817 2202 94-95 1.6051148158769095 2202 96-97 1.271633896237816 2202 98-99 1.2109771748547757 2202 100-101 0.7889321968434437 2305 1 5.599291867107073 2305 2 6.941035597297287 2305 3 6.664248296343747 2305 4 7.692710683050542 2305 5 7.7751266619488 2305 6 6.016392916178354 2305 7 2.739170440461848 2305 8 5.799692746558769 2305 9 6.286155886623458 2305 10-11 4.6748805009670065 2305 12-13 5.170827857991085 2305 14-15 5.50367101615959 2305 16-17 4.897735038917755 2305 18-19 4.319302196488842 2305 20-21 4.986456051095406 2305 22-23 5.020519338173017 2305 24-25 5.222704504341767 2305 26-27 5.988845255670132 2305 28-29 5.123190230905617 2305 30-31 5.452300059715206 2305 32-33 5.464112705088869 2305 34-35 5.369828326885241 2305 36-37 4.657801452415239 2305 38-39 4.533380072414687 2305 40-41 4.006212888038672 2305 42-43 3.989768298262298 2305 44-45 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1209 42-43 -22.773505129195193 1209 44-45 -25.489011642089295 1209 46-47 -19.29988177725727 1209 48-49 -18.76565881354122 1209 50-51 -21.03067078816386 1209 52-53 -23.53931999917976 1209 54-55 -20.570369292836354 1209 56-57 -24.33164905366457 1209 58-59 -24.553975996538938 1209 60-61 -24.29994334294644 1209 62-63 -24.387305045365142 1209 64-65 -24.25905833695754 1209 66-67 -12.123608661531787 1209 68-69 -23.895183502113763 1209 70-71 -23.739196738559514 1209 72-73 -14.0876922584999 1209 74-75 -14.912283839022173 1209 76-77 -12.412658816510096 1209 78-79 -13.046290324079518 1209 80-81 -22.820732899033082 1209 82-83 -14.686764953451165 1209 84-85 -12.056474250540976 1209 86-87 -12.56942374107735 1209 88-89 -9.823917689369658 1209 90-91 -12.436160121826756 1209 92-93 -16.43022112558944 1209 94-95 -21.637563865261736 1209 96-97 -17.516790716540434 1209 98-99 -9.312510725389691 1209 100-101 -4.749707252371731 1212 1 -20.4192122232708 1212 2 -20.164340327907233 1212 3 -21.10201582519891 1212 4 -23.04531035317852 1212 5 -23.226042017443486 1212 6 -24.277724730880472 1212 7 -23.83601460044193 1212 8 -23.466376595085173 1212 9 -24.360318597209808 1212 10-11 -24.054180659921187 1212 12-13 -24.328490412363415 1212 14-15 -24.622254188359303 1212 16-17 -25.64920691667243 1212 18-19 -20.206420004897968 1212 20-21 -25.953259570252467 1212 22-23 -26.135304580798547 1212 24-25 -22.324678928148394 1212 26-27 -24.820562613437776 1212 28-29 -19.695456431823096 1212 30-31 -14.560596892823487 1212 32-33 -24.886393722647792 1212 34-35 -21.10616500521612 1212 36-37 -25.279187250350635 1212 38-39 -25.511516694238992 1212 40-41 -20.032303793144212 1212 42-43 -16.14720462969165 1212 44-45 -13.198238109936453 1212 46-47 -16.67509794522454 1212 48-49 -19.346770367639714 1212 50-51 -11.42984255694476 1212 52-53 -4.4279500084994226 1212 54-55 -3.3528674961980336 1212 56-57 -3.452338708836983 1212 58-59 -2.287899575290105 1212 60-61 -13.235171674726498 1212 62-63 -11.076960217778936 1212 64-65 -15.236691142176552 1212 66-67 -11.785015346803924 1212 68-69 -7.4124248814241085 1212 70-71 -14.416270363909002 1212 72-73 -10.804673474424082 1212 74-75 -7.601860924328843 1212 76-77 -17.92188325282276 1212 78-79 -9.147434077999746 1212 80-81 -6.868729171167285 1212 82-83 -4.4425161006332985 1212 84-85 -3.950508141615174 1212 86-87 -2.7306260031910092 1212 88-89 -2.590373753627098 1212 90-91 -2.9914426193952615 1212 92-93 -2.797444475031149 1212 94-95 -2.61146880468392 1212 96-97 -12.249986752000869 1212 98-99 -9.556555789903609 1212 100-101 -6.098245563303163 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 609.0 3 1146.0 4 693.0 5 3519.0 6 4152.0 7 4269.0 8 8471.0 9 15840.0 10 21267.0 11 28779.0 12 38397.0 13 47614.0 14 54743.0 15 59251.0 16 63464.0 17 69861.0 18 77131.0 19 84214.0 20 90182.0 21 99105.0 22 106753.0 23 113743.0 24 116642.0 25 120948.0 26 125083.0 27 129029.0 28 129587.0 29 130747.0 30 134987.0 31 142225.0 32 154128.0 33 171029.0 34 187785.0 35 199331.0 36 202390.0 37 173902.0 38 57567.0 39 183.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.94370709982043 21.40293873056976 7.82707541110624 21.826278758503577 2 22.768055096605462 22.056329416017782 32.83600795325205 22.339607534124706 3 21.200696510102773 23.252204971182433 29.91278303557097 25.634315483143823 4 24.177589521172642 24.51534890006811 27.472068256059806 23.834993322699443 5 21.988665646969483 27.658749649111332 26.967286965147885 23.385297738771303 6 22.04907449556708 27.279528707146767 26.720904226825482 23.950492570460668 7 24.923777116031903 26.54949375743898 26.48801385788285 22.03871526864626 8 22.42306134137994 26.37344062137658 27.678429235991786 23.525068801251695 9 23.265915185552398 26.0752190895722 27.149672946014462 23.509192778860935 10-11 24.017760488707378 26.578620060080638 25.972052508832792 23.431566942379188 12-13 23.627247718158397 26.315118802820635 26.151093372557483 23.906540106463485 14-15 23.445388889727987 26.781650763340963 26.18106524761722 23.59189509931383 16-17 23.99885978476738 26.617858418742912 25.891788653563474 23.491493142926238 18-19 24.058416140108708 26.539919799045514 25.589562231848994 23.812101828996784 20-21 23.673850166221342 26.76720412462366 25.932383000118502 23.626562709036488 22-23 23.862066565530398 26.50799037769013 25.99625430464696 23.633688752132503 24-25 23.77282742265326 26.444116615721953 26.07162979626955 23.711426165355235 26-27 23.468991150116008 26.60222162786745 26.27935962518758 23.649427596828957 28-29 23.96186343133828 26.57799341215032 25.924678801019702 23.5354643554917 30-31 23.927746890939673 26.363344783062807 25.861644416801543 23.847263909195977 32-33 23.562661233609695 26.600748161329417 26.1208808586319 23.71570974642899 34-35 23.974870209899272 26.55153941848821 25.93164615991389 23.541944211698627 36-37 23.929309889216384 26.451517430633192 25.882061185079785 23.737111495070636 38-39 23.62001557470502 26.591794017330457 26.24113928807288 23.54705111989165 40-41 24.021568366183494 26.51624449361175 25.92619425093572 23.535992889269036 42-43 23.924321615996995 26.442042474848414 25.89772013854612 23.735915770608464 44-45 23.567220404549158 26.539235695370987 26.293825865748683 23.599718034331175 46-47 24.048065627404096 26.532578688104202 25.84243705942879 23.57691862506291 48-49 23.92874283391615 26.370597634822996 25.94760004567767 23.753059485583186 50-51 23.69021753239781 26.56793612648039 26.103038389559625 23.638807951562175 52-53 23.910987274038593 26.56581005449366 25.978792380615634 23.54441029085211 54-55 23.80243253270479 26.40297024505292 26.00939062103416 23.785206601208127 56-57 23.57801917175045 26.532445038054313 26.280703213602084 23.608832576593148 58-59 23.986206330835298 26.456941886382307 25.94315590742528 23.613695875357113 60-61 23.94848486448155 26.263527410106942 25.88675711521256 23.901230610198954 62-63 23.643538654312366 26.560084735524548 26.174983874754748 23.62139273540834 64-65 23.93102457249605 26.512078683080265 25.976145997988215 23.580750746435474 66-67 24.00226308021972 26.310179704181273 25.908317962136245 23.779239253462762 68-69 23.755065311686348 26.47700186668435 26.112968995099735 23.654963826529567 70-71 24.083586853523514 26.425044252172935 25.864731834779782 23.626637059523773 72-73 23.919667859073524 26.392136716123836 25.8881893919641 23.800006032838542 74-75 23.71356013084873 26.50974170344398 26.070356861178496 23.706341304528795 76-77 24.132098560567627 26.493458040501146 25.777727905899372 23.59671549303186 78-79 24.105848220480418 26.301858893651474 25.740798145434685 23.851494740433417 80-81 23.89729842823225 26.448884866912355 25.972783922053843 23.681032782801555 82-83 24.086751675492668 26.422938274943597 25.864226719651747 23.626083329911992 84-85 24.1146178567921 26.264271099816934 25.707969394563996 23.91314164882697 86-87 23.8883422894386 26.450771379150705 25.99644009228782 23.664446239122878 88-89 24.21051002483963 26.341231615257897 25.794298714463693 23.65395964543878 90-91 24.227438220563986 26.23740101289389 25.578911010352734 23.956249756189393 92-93 24.018924135907806 26.434120853726313 25.7892801797638 23.75767483060208 94-95 24.28392965639307 26.36756647597031 25.58655734134479 23.76194652629183 96-97 24.310553035204236 26.2509551951132 25.580350814954656 23.858140954727908 98-99 24.163026346413222 26.397161387057544 25.703250503613162 23.736561762916068 100-101 24.65789777498731 26.236962507373747 25.286259451440824 23.81888026619812 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 212.0 1 242.0 2 262.5 3 265.5 4 292.0 5 335.0 6 437.5 7 556.0 8 691.5 9 866.5 10 1170.0 11 1598.0 12 2203.0 13 2915.0 14 3741.5 15 4764.5 16 6017.5 17 7501.5 18 9184.0 19 11136.5 20 13450.5 21 16197.5 22 19289.0 23 22542.0 24 26240.0 25 30394.5 26 34630.0 27 39298.0 28 44199.5 29 49002.0 30 53641.0 31 58270.0 32 62592.0 33 66310.5 34 69788.5 35 72890.5 36 75384.0 37 77180.5 38 78356.0 39 79339.5 40 79652.0 41 79501.0 42 79505.5 43 79383.0 44 79071.5 45 79144.0 46 78958.0 47 78153.0 48 77153.5 49 75702.0 50 73792.0 51 71989.0 52 70428.5 53 68475.0 54 66803.0 55 65266.0 56 63136.0 57 61036.5 58 58924.0 59 56620.5 60 54336.5 61 51993.0 62 50200.0 63 48430.5 64 46438.0 65 44682.0 66 42690.5 67 40839.0 68 38815.0 69 36227.5 70 33594.0 71 30831.0 72 27723.0 73 24516.5 74 21222.5 75 17742.5 76 14685.0 77 12075.5 78 9666.0 79 7632.0 80 6060.5 81 4854.0 82 3864.0 83 3053.5 84 2380.5 85 1862.5 86 1480.5 87 1146.5 88 915.5 89 739.0 90 565.5 91 432.0 92 354.5 93 273.0 94 180.5 95 117.0 96 79.0 97 46.0 98 28.5 99 14.5 100 3.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.326133895655282 2 10.610250173095773 3 9.364307746296193 4 11.266246860765358 5 8.82592782174512 6 5.092802687229035 7 9.40141998494051 8 9.639272827340358 9 5.148565719273686 10-11 6.057042394421046 12-13 5.148202801974017 14-15 7.020856068261273 16-17 2.5207131104032294 18-19 2.973996817688652 20-21 2.93166172573172 22-23 2.9676063174118883 24-25 3.297703269979544 26-27 4.1907165123584385 28-29 1.6399917191739624 30-31 2.1494329338297624 32-33 2.9125060039144572 34-35 1.942002028549915 36-37 2.968931754506328 38-39 1.3223128498601664 40-41 1.1194736373717717 42-43 0.546727022443437 44-45 1.2404513302654725 46-47 1.3030940119907877 48-49 1.2034179866863 50-51 1.5376648196805949 52-53 2.3585364144906884 54-55 1.7128276433160414 56-57 1.7313206465860844 58-59 1.5120712605474813 60-61 1.3608294206640692 62-63 0.605961437354478 64-65 1.1744634977779995 66-67 0.5462378730395365 68-69 0.7875778773188048 70-71 0.9714980531853724 72-73 0.08711593093336649 74-75 0.10829136641834708 76-77 0.4423015142172063 78-79 0.8420312512820448 80-81 0.2984442524313881 82-83 0.4589957100019377 84-85 0.06750261773826152 86-87 0.1020744352849027 88-89 0.0838023381972667 90-91 0.09109224221668624 92-93 0.1736953754237454 94-95 0.45599769752641883 96-97 0.36886598758002326 98-99 0.06781819799884245 100-101 0.22756492590491062 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3168766.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.94420302611914 #Duplication Level Percentage of deduplicated Percentage of total 1 98.56812931967194 96.54176869990717 2 1.111634980762991 2.177564044935729 3 0.14925820456228567 0.43856927672887597 4 0.07685832476228384 0.30111309459058117 5 0.05386875807575639 0.26380662888683903 6 0.021403470582768713 0.12578075209333608 7 0.00918448821626771 0.06296971649815865 8 0.0016093122798973203 0.012609824693975187 9 0.004296908852917177 0.03787715817673639 >10 0.00375623223289009 0.03794080348859794 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 3.1558026058093276E-5 0.0 4 3.1558026058093276E-5 6.311605211618655E-5 0.0 5 3.1558026058093276E-5 6.311605211618655E-5 0.0 6 3.1558026058093276E-5 6.311605211618655E-5 0.0 7 3.1558026058093276E-5 6.311605211618655E-5 0.0 8 3.1558026058093276E-5 6.311605211618655E-5 0.0 9 3.1558026058093276E-5 6.311605211618655E-5 0.0 10-11 3.1558026058093276E-5 6.311605211618655E-5 0.0 12-13 4.7337039087139915E-5 9.467407817427983E-5 0.0 14-15 6.311605211618655E-5 9.467407817427983E-5 0.0 16-17 6.311605211618655E-5 9.467407817427983E-5 1.5779013029046638E-5 18-19 9.467407817427983E-5 9.467407817427983E-5 3.1558026058093276E-5 20-21 1.262321042323731E-4 9.467407817427983E-5 3.1558026058093276E-5 22-23 1.4201111726141975E-4 9.467407817427983E-5 3.1558026058093276E-5 24-25 1.577901302904664E-4 9.467407817427983E-5 3.1558026058093276E-5 26-27 1.577901302904664E-4 9.467407817427983E-5 6.311605211618655E-5 28-29 1.8934815634855966E-4 9.467407817427983E-5 9.467407817427983E-5 30-31 2.051271693776063E-4 9.467407817427983E-5 9.467407817427983E-5 32-33 2.3668519543569957E-4 9.467407817427983E-5 9.467407817427983E-5 34-35 2.9980124755188615E-4 1.1045309120332646E-4 9.467407817427983E-5 36-37 5.049284169294924E-4 1.262321042323731E-4 1.262321042323731E-4 38-39 5.996024951037723E-4 1.262321042323731E-4 1.577901302904664E-4 40-41 8.67845716597565E-4 1.262321042323731E-4 1.577901302904664E-4 42-43 0.0013412161074689644 1.262321042323731E-4 1.577901302904664E-4 44-45 0.0019408186025727365 1.4201111726141975E-4 1.577901302904664E-4 46-47 0.002919117410373628 1.577901302904664E-4 1.8934815634855966E-4 48-49 0.004276112530871639 1.577901302904664E-4 1.8934815634855966E-4 50-51 0.005522654560166323 1.7356914331951304E-4 1.8934815634855966E-4 52-53 0.007731716384232852 1.8934815634855966E-4 1.8934815634855966E-4 54-55 0.010161684390706034 1.8934815634855966E-4 2.3668519543569957E-4 56-57 0.013128138840166802 1.8934815634855966E-4 2.524642084647462E-4 58-59 0.017767168670706514 2.2090618240665292E-4 2.524642084647462E-4 60-61 0.022784894813943347 2.524642084647462E-4 2.9980124755188615E-4 62-63 0.028986046934358675 2.524642084647462E-4 3.4713828663902603E-4 64-65 0.036749321344649616 2.524642084647462E-4 3.4713828663902603E-4 66-67 0.045380441471538135 2.524642084647462E-4 3.4713828663902603E-4 68-69 0.05595238020099938 2.524642084647462E-4 3.786963126971193E-4 70-71 0.06914363509328236 2.524642084647462E-4 3.786963126971193E-4 72-73 0.08372344313212146 2.524642084647462E-4 3.786963126971193E-4 74-75 0.09844526228822198 2.524642084647462E-4 3.786963126971193E-4 76-77 0.11616509391984135 2.6824322149379286E-4 4.102543387552126E-4 78-79 0.13748254052208336 3.3135927360997944E-4 4.102543387552126E-4 80-81 0.1631392157073132 3.9447532572616596E-4 4.102543387552126E-4 82-83 0.19095761567752242 4.102543387552126E-4 4.7337039087139913E-4 84-85 0.22238940963138332 4.102543387552126E-4 4.891494039004458E-4 86-87 0.25765550375130253 4.102543387552126E-4 5.049284169294924E-4 88-89 0.2952884498255788 4.102543387552126E-4 5.049284169294924E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAA 735 2.5954476E-4 8.403222 1 GTTAAAG 1665 3.2923708E-10 7.756291 1 GTATTAA 1685 3.4015102E-9 7.3310013 1 GTCTCGG 1235 3.8278613E-6 7.2743382 1 GTCCGAT 795 0.005167062 7.0627427 1 GTTCTAG 875 0.0016740991 7.0587068 1 GTAGATA 1215 2.3750436E-5 6.931951 1 GTCCCAT 825 0.0073985583 6.8059154 1 GTGTATA 1120 4.6429536E-4 6.5172715 1 GTACTGG 1210 1.6798332E-4 6.496556 1 GTCCAAA 1660 1.0565473E-6 6.42667 1 GTTCGTT 1065 0.0018731361 6.326626 1 GTCAAGT 1365 1.1921283E-4 6.170198 1 GTCATAG 1025 0.0087578045 6.0257254 1 GTCCAAC 1025 0.0087578045 6.0257254 1 GTCTCAA 1965 4.8083166E-7 6.000635 1 GTATTTC 1880 1.389828E-6 5.9732766 1 GTATTCC 1035 0.00968133 5.967506 1 GTCCAAG 1225 0.0013696243 5.958648 1 GTTCAAC 1980 5.541624E-7 5.955176 1 >>END_MODULE