FastQCFastQC Report
Thu 11 Sep 2014
LONLib36_CCAACA_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib36_CCAACA_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3168766
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA7352.5954476E-48.4032221
GTTAAAG16653.2923708E-107.7562911
GTATTAA16853.4015102E-97.33100131
GTCTCGG12353.8278613E-67.27433821
GTCCGAT7950.0051670627.06274271
GTTCTAG8750.00167409917.05870681
GTAGATA12152.3750436E-56.9319511
GTCCCAT8250.00739855836.80591541
GTGTATA11204.6429536E-46.51727151
GTACTGG12101.6798332E-46.4965561
GTCCAAA16601.0565473E-66.426671
GTTCGTT10650.00187313616.3266261
GTCAAGT13651.1921283E-46.1701981
GTCATAG10250.00875780456.02572541
GTCCAAC10250.00875780456.02572541
GTCTCAA19654.8083166E-76.0006351
GTATTTC18801.389828E-65.97327661
GTATTCC10350.009681335.9675061
GTCCAAG12250.00136962435.9586481
GTTCAAC19805.541624E-75.9551761
Produced by FastQC (version 0.11.2)