##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib36_CCAACA_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3168766 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.213405786353427 33.0 33.0 33.0 27.0 33.0 2 31.585221187048838 33.0 33.0 33.0 27.0 33.0 3 31.792310003326214 33.0 33.0 33.0 27.0 33.0 4 35.612238013157175 37.0 37.0 37.0 33.0 37.0 5 35.1489002343499 37.0 37.0 37.0 33.0 37.0 6 34.94863236982472 37.0 37.0 37.0 33.0 37.0 7 34.74365194526828 37.0 33.0 37.0 27.0 37.0 8 34.77734708085103 37.0 33.0 37.0 27.0 37.0 9 34.67582270196032 37.0 33.0 37.0 27.0 37.0 10-11 34.52690227047374 37.0 33.0 37.0 27.0 37.0 12-13 34.54434707390827 37.0 33.0 37.0 27.0 37.0 14-15 35.461278933187245 37.0 33.0 40.0 27.0 40.0 16-17 35.3549717776573 37.0 33.0 40.0 27.0 40.0 18-19 35.557172255698276 37.0 33.0 40.0 27.0 40.0 20-21 35.214583216305655 37.0 33.0 40.0 27.0 40.0 22-23 35.238113196114824 37.0 33.0 40.0 27.0 40.0 24-25 35.06545134604448 37.0 33.0 40.0 27.0 40.0 26-27 34.667584163677596 37.0 33.0 40.0 27.0 40.0 28-29 34.26864258831356 37.0 33.0 40.0 24.5 40.0 30-31 34.15489941510354 37.0 33.0 40.0 24.5 40.0 32-33 34.1291931938174 37.0 33.0 38.5 24.5 40.0 34-35 33.95665536678947 37.0 33.0 37.0 24.5 40.0 36-37 33.62002274702518 37.0 33.0 37.0 22.0 40.0 38-39 33.48417128307992 37.0 33.0 37.0 22.0 40.0 40-41 33.17148426232798 37.0 33.0 37.0 22.0 40.0 42-43 32.76029801506327 37.0 33.0 37.0 22.0 40.0 44-45 32.578193372435834 37.0 33.0 37.0 22.0 40.0 46-47 32.405519845895846 37.0 33.0 37.0 22.0 40.0 48-49 32.4028378870513 37.0 33.0 37.0 22.0 40.0 50-51 32.686799687954235 37.0 33.0 37.0 22.0 40.0 52-53 32.53161404155435 37.0 33.0 37.0 22.0 40.0 54-55 32.143631464109376 37.0 27.0 37.0 22.0 40.0 56-57 32.16438417983531 33.0 27.0 37.0 22.0 40.0 58-59 31.858118901805938 33.0 27.0 37.0 22.0 40.0 60-61 31.746980843647023 33.0 27.0 37.0 22.0 40.0 62-63 31.704462872929085 33.0 27.0 37.0 22.0 40.0 64-65 31.37500970409301 33.0 27.0 37.0 18.5 40.0 66-67 31.015426036507588 33.0 27.0 37.0 15.0 38.5 68-69 30.823730436390697 33.0 27.0 37.0 15.0 37.0 70-71 30.66726621656506 33.0 27.0 37.0 15.0 37.0 72-73 30.45484677631608 33.0 27.0 37.0 15.0 37.0 74-75 30.11303122414214 33.0 27.0 37.0 15.0 37.0 76-77 29.054640197477504 33.0 27.0 33.0 15.0 37.0 78-79 30.014327186040244 33.0 27.0 37.0 15.0 37.0 80-81 29.74699204674627 33.0 27.0 37.0 15.0 37.0 82-83 29.741217085767772 33.0 27.0 37.0 15.0 37.0 84-85 29.634261255012206 33.0 27.0 37.0 15.0 37.0 86-87 29.435851684851453 33.0 27.0 37.0 15.0 37.0 88-89 29.317446602242008 33.0 27.0 37.0 15.0 37.0 90-91 29.24055184257847 33.0 27.0 33.0 15.0 37.0 92-93 29.02680144258049 33.0 27.0 33.0 15.0 37.0 94-95 28.938828869029773 33.0 27.0 33.0 15.0 37.0 96-97 28.806907326069517 33.0 27.0 33.0 15.0 37.0 98-99 28.529170503596667 33.0 27.0 33.0 15.0 37.0 100-101 27.713991345526935 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.1288280659106178 2306 2 0.5071361901214146 2306 3 0.42864045329538314 2306 4 0.37784693165579597 2306 5 0.637612467327358 2306 6 0.6558898818645602 2306 7 0.8206093646631416 2306 8 0.7052169441064109 2306 9 0.9553284774763213 2306 10-11 1.1265087480310996 2306 12-13 1.3915612599958251 2306 14-15 1.4342750419135726 2306 16-17 1.3006628326248943 2306 18-19 1.2728655218855849 2306 20-21 1.5272650777877175 2306 22-23 1.2523754754941905 2306 24-25 1.2544548646313132 2306 26-27 1.3210902086951037 2306 28-29 1.6083170825374467 2306 30-31 1.5715601495705798 2306 32-33 1.1225339440088575 2306 34-35 1.0447917702086542 2306 36-37 1.2774520860899514 2306 38-39 1.0802784092980389 2306 40-41 1.2009393077500548 2306 42-43 1.2462466857399335 2306 44-45 1.0467541929189679 2306 46-47 0.967412937538505 2306 48-49 0.8933640453479228 2306 50-51 1.098941724490743 2306 52-53 1.2129341012778738 2306 54-55 1.6579842969501044 2306 56-57 1.1921225415919636 2306 58-59 1.1005218333422278 2306 60-61 0.972294771492642 2306 62-63 0.6667549475121248 2306 64-65 0.7114408407695514 2306 66-67 0.9099306578535682 2306 68-69 0.7968164882983402 2306 70-71 0.8226559527420463 2306 72-73 0.5480214929169982 2306 74-75 0.6725048385954651 2306 76-77 0.7541779437134295 2306 78-79 0.7491651909094479 2306 80-81 1.301391873175561 2306 82-83 1.1507161102456003 2306 84-85 0.849132776663243 2306 86-87 0.7622700882688562 2306 88-89 0.629793049087862 2306 90-91 0.1768333482266229 2306 92-93 0.031200246074039484 2306 94-95 0.026871570569561243 2306 96-97 0.048890650211415476 2306 98-99 0.22972004068841656 2306 100-101 0.17382017254048776 2307 1 0.6276380906804597 2307 2 0.3877689413638983 2307 3 0.34081064804811945 2307 4 0.30422441444668635 2307 5 0.46118005250986016 2307 6 0.5880862032086114 2307 7 0.65505448268582 2307 8 0.6485093645564319 2307 9 0.9589157606655832 2307 10-11 1.150788553555799 2307 12-13 1.332399672659463 2307 14-15 1.3046333557758842 2307 16-17 1.2889558356979407 2307 18-19 1.1835392057760288 2307 20-21 1.3695516687423037 2307 22-23 1.2325757175296417 2307 24-25 1.230940591909608 2307 26-27 1.3441980675515808 2307 28-29 1.5322552908833984 2307 30-31 1.5927434494252068 2307 32-33 1.2358817363824102 2307 34-35 1.276071247032604 2307 36-37 1.3569389539552361 2307 38-39 1.1083702841316665 2307 40-41 1.1681214256922274 2307 42-43 1.1868357734079638 2307 44-45 0.9879067532742383 2307 46-47 0.8277434347319925 2307 48-49 0.99977917458056 2307 50-51 0.8494373847454568 2307 52-53 1.158924225592223 2307 54-55 1.5137381235073661 2307 56-57 1.098214046118926 2307 58-59 1.1058421721421752 2307 60-61 0.9600498223094434 2307 62-63 0.775920974882645 2307 64-65 0.8508707339721546 2307 66-67 0.9323891279093388 2307 68-69 0.6919707153340653 2307 70-71 0.7784881194104081 2307 72-73 0.7324755460952872 2307 74-75 0.8622027293759125 2307 76-77 0.9805889375945895 2307 78-79 0.936124983830986 2307 80-81 1.4838144179162924 2307 82-83 1.1326179380352919 2307 84-85 0.9097990978893087 2307 86-87 0.9280040138978762 2307 88-89 0.7772677173057296 2307 90-91 0.6793835083432853 2307 92-93 0.6327610848484788 2307 94-95 0.5487870503876167 2307 96-97 0.5618055107083073 2307 98-99 0.6258378142323302 2307 100-101 0.6426717453332031 1101 1 1.039523088836809 1101 2 0.5537628254995255 1101 3 0.35056940585181806 1101 4 0.27038984645906794 1101 5 0.5558876402485708 1101 6 0.6268697903080849 1101 7 0.7299818550925394 1101 8 0.6215797101317264 1101 9 0.8364617536666188 1101 10-11 0.9883122125366981 1101 12-13 1.1967155540432728 1101 14-15 1.3291531532773533 1101 16-17 1.3342885145399634 1101 18-19 1.0274034179405902 1101 20-21 1.3150909902442578 1101 22-23 0.987710495499428 1101 24-25 1.1911562778836498 1101 26-27 1.2154339491452149 1101 28-29 1.4050329224644642 1101 30-31 1.4174457302990433 1101 32-33 0.8246348693141243 1101 34-35 0.8566606762352578 1101 36-37 1.0082142495946513 1101 38-39 0.846258980075774 1101 40-41 1.023524537386983 1101 42-43 1.0988395337314785 1101 44-45 1.1159059239526812 1101 46-47 0.9885645712874762 1101 48-49 0.9396991589705763 1101 50-51 0.9021937471489849 1101 52-53 1.03151401241346 1101 54-55 1.4539516004906403 1101 56-57 1.1923451298118835 1101 58-59 1.222712126815324 1101 60-61 1.035538126781404 1101 62-63 0.7987590914231006 1101 64-65 0.772045327224383 1101 66-67 0.8861751761665353 1101 68-69 0.8420471440188777 1101 70-71 0.8689989551338222 1101 72-73 0.8659946740842024 1101 74-75 0.9168917728271495 1101 76-77 0.8637703766529441 1101 78-79 0.8809037462600173 1101 80-81 1.5379421162524167 1101 82-83 1.3120357161400236 1101 84-85 1.0946334882241722 1101 86-87 0.9492681212551553 1101 88-89 0.9004373332242785 1101 90-91 0.6505285045062585 1101 92-93 0.6973438191734296 1101 94-95 0.7969076940795929 1101 96-97 0.7745151092564555 1101 98-99 0.8864967936001165 1101 100-101 0.7978143444024894 2201 1 1.4709409132502742 2201 2 0.8000444082536227 2201 3 0.29758796742514093 2201 4 0.20933422320698014 2201 5 0.3166543181576884 2201 6 0.4425555973285 2201 7 0.5876818626244642 2201 8 0.6823835255861184 2201 9 0.6429884046443846 2201 10-11 0.8921442737866556 2201 12-13 1.1260116630465404 2201 14-15 1.1327675319278327 2201 16-17 1.029422169917268 2201 18-19 0.8651670767243047 2201 20-21 1.132973210883378 2201 22-23 0.9921215104928294 2201 24-25 0.8755931855895795 2201 26-27 1.1226456701603595 2201 28-29 1.36878052516456 2201 30-31 1.5194998499878665 2201 32-33 1.0598977348043448 2201 34-35 0.9989981072728185 2201 36-37 1.0565860707317185 2201 38-39 0.7229042404833876 2201 40-41 1.1283384911681011 2201 42-43 1.1206741137330312 2201 44-45 0.9417950094592626 2201 46-47 0.8205480812237838 2201 48-49 0.8960797100621107 2201 50-51 0.9497808529225296 2201 52-53 1.1126023121584652 2201 54-55 1.4257310762226751 2201 56-57 1.0896550280812747 2201 58-59 1.1791727545547772 2201 60-61 0.9630844326187002 2201 62-63 0.8269225263458537 2201 64-65 0.8019795738553199 2201 66-67 0.7838000131135132 2201 68-69 0.8072834377028038 2201 70-71 0.981932289471974 2201 72-73 0.8743765877417893 2201 74-75 0.803455622818074 2201 76-77 0.739586799548249 2201 78-79 0.7949487061402287 2201 80-81 1.4012466997395414 2201 82-83 1.1959024524309925 2201 84-85 1.1655860083714273 2201 86-87 1.229293201412851 2201 88-89 1.1227500007058246 2201 90-91 0.8992220910445923 2201 92-93 0.9976615091701184 2201 94-95 0.9431946258022137 2201 96-97 0.6988545074345538 2201 98-99 0.8750734198160082 2201 100-101 0.9350111872438163 2304 1 -1.7731791825341077 2304 2 -0.26091489582156413 2304 3 -0.07410904170568244 2304 4 0.037966057691853905 2304 5 0.4919210412571431 2304 6 0.5524712153610025 2304 7 0.553967080934413 2304 8 0.64082030367274 2304 9 0.9280838371851132 2304 10-11 0.909307454628987 2304 12-13 1.2153045989109401 2304 14-15 1.325638239656847 2304 16-17 1.176900916735022 2304 18-19 0.9882329580873659 2304 20-21 1.1803736876807562 2304 22-23 1.012280615960698 2304 24-25 1.129946919352463 2304 26-27 1.1891932269240115 2304 28-29 1.5333641789354786 2304 30-31 1.5525161293320053 2304 32-33 0.9212612078609865 2304 34-35 1.054819319293209 2304 36-37 1.139514465895779 2304 38-39 1.0121848665907152 2304 40-41 1.0095596141168173 2304 42-43 0.9898734227904669 2304 44-45 1.1500115094928844 2304 46-47 1.0678897667534386 2304 48-49 1.0704940244519818 2304 50-51 1.073384725435048 2304 52-53 1.204975769548163 2304 54-55 1.5457586244297659 2304 56-57 1.180028211865789 2304 58-59 0.9804474680157966 2304 60-61 0.5956608466122617 2304 62-63 0.4210368387735457 2304 64-65 0.5053022780863152 2304 66-67 0.76234281032459 2304 68-69 0.7829986647237028 2304 70-71 0.6575048163288066 2304 72-73 0.4278881723019907 2304 74-75 0.719895420359034 2304 76-77 0.6816829461539591 2304 78-79 0.6722539338185847 2304 80-81 1.3115912961566032 2304 82-83 1.0556280920808376 2304 84-85 0.745561492883386 2304 86-87 0.6135908829872001 2304 88-89 0.49588799170668807 2304 90-91 0.3448518507038081 2304 92-93 0.3937046061442828 2304 94-95 0.2859787730697292 2304 96-97 0.23759410829735117 2304 98-99 0.5431869301417969 2304 100-101 0.6917660254007814 1102 1 1.526212529558741 1102 2 0.19752816563659437 1102 3 0.2742072178909112 1102 4 0.21532722336964838 1102 5 0.33898598104637756 1102 6 0.2493786197522283 1102 7 -4.980066418034394 1102 8 -1.1076922775609788 1102 9 0.2621775615685067 1102 10-11 0.8010173814316062 1102 12-13 1.0102891252429202 1102 14-15 0.9341442005828853 1102 16-17 0.6950473914009194 1102 18-19 0.981779124246053 1102 20-21 1.2657182176304929 1102 22-23 0.788067792734509 1102 24-25 1.2027502484677655 1102 26-27 1.2118530859122671 1102 28-29 1.0748475186796824 1102 30-31 1.3761796590038031 1102 32-33 1.0782556399085905 1102 34-35 1.3146344817488043 1102 36-37 1.3941910983663561 1102 38-39 1.1196664594114338 1102 40-41 1.2033496115493563 1102 42-43 1.2852450791096715 1102 44-45 1.2776865055550388 1102 46-47 1.0914775035325945 1102 48-49 1.0049501266883354 1102 50-51 0.85338096493075 1102 52-53 1.115852284946321 1102 54-55 1.4773872581424996 1102 56-57 1.2512783616969188 1102 58-59 1.153514144630691 1102 60-61 1.0426681228421586 1102 62-63 0.8737225668898958 1102 64-65 0.5984906197625044 1102 66-67 0.6685419296821742 1102 68-69 0.8904786369060673 1102 70-71 0.838191282583594 1102 72-73 0.8304253190472402 1102 74-75 0.7451452931856721 1102 76-77 0.7158400811296346 1102 78-79 0.9366713040448751 1102 80-81 1.396509878713882 1102 82-83 1.2276511710930045 1102 84-85 0.9080360823647773 1102 86-87 0.8681835383414374 1102 88-89 0.7828888171726014 1102 90-91 0.4199502063106344 1102 92-93 0.49441517191474205 1102 94-95 0.4652088620159702 1102 96-97 0.33639338045620093 1102 98-99 0.2894622824576665 1102 100-101 0.5378995344611823 2202 1 1.5456683583067203 2202 2 0.9706474736871193 2202 3 0.4659352685671756 2202 4 -0.2388389349297242 2202 5 0.07340008348133154 2202 6 0.5852080505974158 2202 7 0.6987473646728191 2202 8 -3.920479747832079 2202 9 -0.4672319372774254 2202 10-11 0.49310984762142596 2202 12-13 0.1939528673947848 2202 14-15 0.29094011549801735 2202 16-17 0.24743793481410847 2202 18-19 0.8279769953156162 2202 20-21 1.1751275051130605 2202 22-23 -2.776012127278001 2202 24-25 -2.996225855209758 2202 26-27 -1.4332977269323095 2202 28-29 0.6560407075764161 2202 30-31 1.380619526091472 2202 32-33 1.161865958614058 2202 34-35 -2.0696660907817197 2202 36-37 -0.22474508473231225 2202 38-39 0.5176140770651259 2202 40-41 1.2993787475832974 2202 42-43 1.3395880238986493 2202 44-45 1.42885211356446 2202 46-47 0.7276609544718085 2202 48-49 0.9609037789017023 2202 50-51 -3.8803790796560875 2202 52-53 -0.2109400408562223 2202 54-55 1.6260067335562312 2202 56-57 1.4904171467463954 2202 58-59 1.5028566555150604 2202 60-61 1.2984915393155916 2202 62-63 1.0980639345829672 2202 64-65 1.1330800487894344 2202 66-67 1.2936599154922384 2202 68-69 1.3026715020510231 2202 70-71 1.3736710967382884 2202 72-73 1.2894855216349654 2202 74-75 0.7345457758935403 2202 76-77 1.3378257397185855 2202 78-79 1.3923075216730005 2202 80-81 1.9622692229563228 2202 82-83 1.8195928647463226 2202 84-85 1.6333076182427995 2202 86-87 1.6957156359352226 2202 88-89 1.5102560682747885 2202 90-91 1.3873531649506852 2202 92-93 1.3478763554723798 2202 94-95 1.2996514747950947 2202 96-97 1.2304253223388635 2202 98-99 1.3523098930591395 2202 100-101 1.3062363398217371 2305 1 1.379413562903796 2305 2 0.6933689736941808 2305 3 0.13371710965565953 2305 4 -0.11503746155920425 2305 5 0.3625544132234779 2305 6 0.4910125234906175 2305 7 0.6363702090401304 2305 8 0.04319043040409554 2305 9 0.5299392267452774 2305 10-11 0.3300370090340934 2305 12-13 0.7832296934692948 2305 14-15 0.5966719178977797 2305 16-17 0.7035089446324037 2305 18-19 0.6767892408163121 2305 20-21 0.8787709063985005 2305 22-23 0.6212841378543317 2305 24-25 0.747095503860109 2305 26-27 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0.9403916815749369 2305 94-95 0.7893881736974393 2305 96-97 0.94397609168157 2305 98-99 1.0858767601646768 2305 100-101 1.1866084137055068 1103 1 1.3061319604331878 1103 2 0.592105825851732 1103 3 0.23645463810241196 1103 4 -0.23437193527364286 1103 5 0.029956157784965853 1103 6 0.02239655922330286 1103 7 0.47322345069657956 1103 8 0.11516317732039738 1103 9 -0.09684044653944568 1103 10-11 0.4615248197662183 1103 12-13 -0.05093670555935148 1103 14-15 0.14663377898124708 1103 16-17 0.3011705176871402 1103 18-19 0.3636984094532991 1103 20-21 0.40430086356286665 1103 22-23 0.18940337551602227 1103 24-25 0.5051758206159889 1103 26-27 0.7188629139132772 1103 28-29 0.45241123945054795 1103 30-31 0.06629456551720381 1103 32-33 -0.011365852799826826 1103 34-35 0.29846430536220225 1103 36-37 0.7290457300114284 1103 38-39 -0.023854842826281697 1103 40-41 -3.404625271823811 1103 42-43 -1.015917022968246 1103 44-45 0.004216080566237679 1103 46-47 -2.849141586140796 1103 48-49 -1.118501930830444 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1208 42-43 -0.01884626819773416 1208 44-45 0.013431570588636532 1208 46-47 -0.0790473560570959 1208 48-49 -1.4557016546368473 1208 50-51 -0.1374917955862216 1208 52-53 -1.5842981311478326 1208 54-55 -0.9218992687587502 1208 56-57 -6.310895573131724 1208 58-59 -2.2733697898643186 1208 60-61 -1.4404614035367374 1208 62-63 0.33403782636750634 1208 64-65 0.005356521069213471 1208 66-67 0.7719577033789555 1208 68-69 0.6466693585549166 1208 70-71 -4.172139082520758 1208 72-73 -0.5499846528331496 1208 74-75 -5.298352171543552 1208 76-77 -1.016083918978211 1208 78-79 -4.1210724471562195 1208 80-81 0.824251860820798 1208 82-83 0.3815621495842869 1208 84-85 -2.790607987581044 1208 86-87 -1.0776884582764765 1208 88-89 -4.998245701572841 1208 90-91 -0.06743998089203274 1208 92-93 -1.9822511590713283 1208 94-95 -4.890135464405418 1208 96-97 -3.494996401221325 1208 98-99 -6.545933414174566 1208 100-101 -3.880346215988695 1209 1 1.7313113135409424 1209 2 1.2523756068254421 1209 3 0.4787651889772526 1209 4 0.4210339711822897 1209 5 0.3718056623092849 1209 6 1.0431567476562762 1209 7 0.9026438838203248 1209 8 0.31495615529845367 1209 9 0.05175988275548349 1209 10-11 -3.032887035297179 1209 12-13 -0.26078207187347147 1209 14-15 -1.1495227091491316 1209 16-17 -0.23590973024863615 1209 18-19 -16.681292638921164 1209 20-21 -13.737975759786963 1209 22-23 -3.1409976104730504 1209 24-25 -2.029300622195926 1209 26-27 -1.5092364087070962 1209 28-29 -2.986572601903564 1209 30-31 -0.6833669955726904 1209 32-33 -0.5372331512934636 1209 34-35 -2.8090793975832185 1209 36-37 -4.093623711073718 1209 38-39 -1.8166573288747365 1209 40-41 -10.321278557050867 1209 42-43 -4.9023133399500125 1209 44-45 -2.6328233818567313 1209 46-47 -2.0840446738522296 1209 48-49 -2.976046609505836 1209 50-51 -2.2337304722705085 1209 52-53 -0.8189786344085661 1209 54-55 -2.5002384110007903 1209 56-57 -0.751632789053776 1209 58-59 -1.8501726219481505 1209 60-61 -1.7527529495717893 1209 62-63 -0.9213417943514841 1209 64-65 0.049772616732152386 1209 66-67 0.2553498356456281 1209 68-69 -1.2870454495482804 1209 70-71 -0.8241970282864735 1209 72-73 0.029325711226729112 1209 74-75 0.6668144040261765 1209 76-77 -16.55979828089232 1209 78-79 -16.21635688763003 1209 80-81 -21.429669025797487 1209 82-83 -9.969116615567128 1209 84-85 -3.1918209627597456 1209 86-87 -2.4369789263592523 1209 88-89 -1.8925429993908764 1209 90-91 -6.066749401870421 1209 92-93 -3.5398333820867762 1209 94-95 -0.6250978780450183 1209 96-97 0.149516385645299 1209 98-99 1.237844620950657 1209 100-101 0.6056728545385894 1212 1 -28.607925743679765 1212 2 -16.240243574415366 1212 3 -5.746153613128435 1212 4 -1.3588683120085463 1212 5 -0.4860427902962883 1212 6 -2.409740855386964 1212 7 -0.39590534660901966 1212 8 -1.4606418141828215 1212 9 -0.07609728005422767 1212 10-11 0.6285894839732009 1212 12-13 0.3640500091034937 1212 14-15 0.0666865236672578 1212 16-17 -2.8220194046468805E-4 1212 18-19 -0.9534300163624962 1212 20-21 -0.5637989756552741 1212 22-23 -0.5490763883018985 1212 24-25 0.4689509884300236 1212 26-27 -0.48000961073558557 1212 28-29 0.341216123587877 1212 30-31 -0.6118842801221049 1212 32-33 0.40196746527725224 1212 34-35 -0.6975475189978795 1212 36-37 -1.0792328286005244 1212 38-39 -0.22439201554863075 1212 40-41 -0.3509662594107468 1212 42-43 0.10858965173846968 1212 44-45 1.0972764680636544 1212 46-47 -1.7605494695433492 1212 48-49 -1.1834973892341694 1212 50-51 0.16010343962691564 1212 52-53 -0.5795337258149189 1212 54-55 -0.18883464605948674 1212 56-57 -1.3777231517080786 1212 58-59 -0.17585807438358358 1212 60-61 -0.016144571015651366 1212 62-63 -4.532713689595411 1212 64-65 -3.8115484901494163 1212 66-67 -1.9573873777492956 1212 68-69 0.11804824228317301 1212 70-71 -0.5606695479733155 1212 72-73 -1.1998472510806906 1212 74-75 -0.2904275085619723 1212 76-77 1.0468890227789167 1212 78-79 -1.1219622722122082 1212 80-81 0.6201714987447211 1212 82-83 0.6266968997239779 1212 84-85 -3.43013710000006 1212 86-87 -1.878194571206393 1212 88-89 -0.7819576889089213 1212 90-91 0.05373456043445657 1212 92-93 -2.1317920606751635 1212 94-95 -1.7615846184733144 1212 96-97 0.43893196395968914 1212 98-99 -0.38486831560706136 1212 100-101 0.17152758458574624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 7.0 10 10.0 11 26.0 12 88.0 13 352.0 14 1143.0 15 2613.0 16 4884.0 17 8102.0 18 12465.0 19 18339.0 20 24943.0 21 33316.0 22 43377.0 23 54981.0 24 68405.0 25 83834.0 26 100462.0 27 119853.0 28 140619.0 29 164539.0 30 191010.0 31 219624.0 32 250217.0 33 280953.0 34 309196.0 35 331395.0 36 336600.0 37 278493.0 38 88643.0 39 274.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.63172168833311 22.11187489930992 8.526380244503315 21.730023167853656 2 21.787662599560573 21.064489436275235 35.199834378670694 21.948013585493488 3 21.395133361399008 22.35948595632737 31.644196243449326 24.601184438824298 4 24.32542288583905 23.869282745348894 28.888244403257673 22.91704996555438 5 21.34919397645645 27.613683055170373 27.69298837465436 23.344134593718817 6 21.406219329543426 27.423861528430944 27.489502222631774 23.68041691939386 7 24.33666607127191 26.666374228958528 27.184146762493665 21.8128129372759 8 22.000330728113088 26.31674285826091 28.276622508572736 23.40630390505326 9 22.874898304261027 25.995355920885295 27.577296651125387 23.55244912372829 10-11 23.453246468814672 26.581435801823172 26.547116448484992 23.418201280877163 12-13 23.389258244103225 26.22767666612923 26.510193887777135 23.87287120199041 14-15 23.096186827330705 26.614528591655016 26.578662889380738 23.71062169163354 16-17 23.570825359636853 26.514066105520406 26.322760506344455 23.592348028498286 18-19 23.78644825752614 26.36790856692224 25.963383420708997 23.88225975484263 20-21 23.239598632401382 26.58971978366342 26.373894443452116 23.796787140483076 22-23 23.468157636127124 26.382872701865644 26.421831085034363 23.727138576972866 24-25 23.53010604127916 26.19169418000572 26.415756165018177 23.86244361369694 26-27 23.39161364392322 26.379385539986227 26.59368031593371 23.635320500156844 28-29 23.779225098981748 26.35019436588249 26.30243129344357 23.56814924169219 30-31 23.809599699062662 26.159299866257086 26.132002173716835 23.899098260963417 32-33 23.37968471007326 26.348790033722906 26.485625634710797 23.785899621493037 34-35 23.8384121768537 26.30666006893535 26.213406101933685 23.64152165227726 36-37 23.793157967486398 26.218834082415675 26.20213988663095 23.785868063466978 38-39 23.443573933827867 26.32789862047245 26.549609532543585 23.6789179131561 40-41 23.783713416726826 26.29891478005657 26.284019140265958 23.633352662950646 42-43 23.62164009586066 26.158984285996507 26.366841224628136 23.8525343935147 44-45 23.348710507497238 26.242565718011363 26.665064570877117 23.74365920361428 46-47 23.795555510663 26.273666706859533 26.336197127704104 23.594580654773367 48-49 23.8088498200403 26.11444837341993 26.273469316910532 23.803232489629238 50-51 23.449367552990307 26.366972479959454 26.516494525388218 23.667165441662025 52-53 23.666447075159468 26.378641504372762 26.37808923830675 23.576822182161017 54-55 23.603093006368233 26.101612113748963 26.473484211333552 23.821810668549247 56-57 23.443130501357572 26.32465329307085 26.571567925271715 23.660648280299863 58-59 23.706663116213992 26.252375589248008 26.413535816533834 23.62742547800417 60-61 23.795361472427413 26.074671959269097 26.288213823999406 23.841752744304088 62-63 23.45613089991482 26.29764910716061 26.537920308920565 23.708299684004 64-65 23.710362528902095 26.271309600884784 26.385347928934888 23.63297994127823 66-67 23.773982378666936 26.088867252992447 26.3182743812138 23.818875987126816 68-69 23.48263259882976 26.30193967013615 26.506226764039415 23.70920096699468 70-71 23.849961118867704 26.24681566051217 26.236543088626256 23.66668013199387 72-73 23.664269022722266 26.103248752823156 26.41643994072398 23.816042283730603 74-75 23.3918978884462 26.207476606302503 26.693165079619156 23.70746042563214 76-77 23.687533139311146 26.34471521330456 26.406857101314824 23.56089454606947 78-79 23.825872416218726 26.142443235628356 26.21046675399849 23.821217594154426 80-81 23.459277572846975 26.20454814732901 26.59366039762719 23.74251388219682 82-83 23.709545198838914 26.25737218456518 26.342094611707285 23.69098800488862 84-85 23.617266403656288 26.059188539181484 26.448544051187767 23.875001005974465 86-87 23.400518759628905 26.284720671594414 26.60177726537583 23.712983303400847 88-89 23.718936197005604 26.268360707320994 26.325469407000558 23.687233688672844 90-91 23.790933971714452 26.01357285882778 26.2121042097832 23.983388959674574 92-93 23.420167157504963 26.199734312926854 26.574530463273295 23.805568066294892 94-95 23.707361014756874 26.225836303919532 26.298525448775774 23.768277232547817 96-97 23.75144828117312 26.064926551511153 26.23310233426156 23.950522833054173 98-99 23.475949002324718 26.190698253549456 26.454085348465544 23.879267395660282 100-101 23.782843644886686 26.04690770181046 26.20842929678216 23.961819356520692 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 2.0 2 4.0 3 8.0 4 17.5 5 36.5 6 68.0 7 107.5 8 168.0 9 258.5 10 401.5 11 613.0 12 918.0 13 1275.5 14 1761.5 15 2431.5 16 3286.0 17 4441.0 18 5911.0 19 7632.5 20 9727.5 21 12224.0 22 15056.5 23 18328.5 24 22103.0 25 26213.5 26 30633.0 27 35580.5 28 40687.0 29 45518.5 30 50540.5 31 55522.0 32 60071.5 33 64403.0 34 68354.0 35 71617.5 36 74381.0 37 76704.0 38 78535.5 39 79379.5 40 79555.5 41 79813.5 42 79991.5 43 79804.5 44 79757.0 45 79851.5 46 79984.5 47 80081.0 48 78969.0 49 77225.0 50 75793.0 51 74543.5 52 73250.5 53 71798.5 54 70153.0 55 68426.5 56 66677.5 57 64478.5 58 62430.5 59 60495.5 60 58073.5 61 56105.5 62 54131.5 63 52127.5 64 50568.5 65 49314.0 66 47677.5 67 45840.0 68 44293.0 69 42032.0 70 39057.5 71 35670.0 72 31792.5 73 27573.0 74 22962.0 75 18297.0 76 14038.0 77 10350.5 78 7334.5 79 4847.0 80 3094.0 81 1957.0 82 1183.5 83 781.5 84 532.0 85 359.0 86 235.5 87 150.0 88 113.5 89 97.0 90 67.0 91 40.5 92 29.5 93 19.5 94 7.5 95 5.5 96 3.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6858505803205411 2 0.0029033383973445814 3 2.524642084647462E-4 4 9.467407817427983E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.17659871382109 14-15 1.5779013029046638E-5 16-17 4.418123648133059E-4 18-19 0.11667002233677083 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0016883543941079901 42-43 0.0 44-45 0.0 46-47 0.02234308244913004 48-49 3.1558026058093276E-5 50-51 7.88950651452332E-5 52-53 1.1045309120332647E-4 54-55 0.002177503798008436 56-57 0.00187770255045655 58-59 0.09380623245768227 60-61 0.002098608732863203 62-63 0.0017356914331951303 64-65 0.0029822334624898146 66-67 0.005159737260498251 68-69 0.005869792846805349 70-71 0.004228775491784499 72-73 0.03824832758240905 74-75 0.044386363650708194 76-77 0.15900511429370298 78-79 2.840222345228395E-4 80-81 0.11805857548332695 82-83 0.0057908977816601166 84-85 0.006201152120415329 86-87 0.007779053423319993 88-89 0.007794832436349039 90-91 0.02374741460871519 92-93 0.011897375823901166 94-95 0.015147852507884772 96-97 0.011203099250623114 98-99 0.01342794008771869 100-101 0.014485133960664812 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3168766.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.67632597675107 #Duplication Level Percentage of deduplicated Percentage of total 1 97.86094729226022 94.60836840820205 2 1.669751910222458 3.228509599459383 3 0.15552204427086833 0.45105899545503536 4 0.09732405911075832 0.3763572985588905 5 0.09095048083866612 0.4396379166650569 6 0.04770085545064013 0.27669260705495813 7 0.042798848019684435 0.28963447678063076 8 0.016302702817509805 0.12608683295101408 9 0.0029019115365024203 0.02524915310927423 >10 0.015631092624656943 0.16453335847643047 >50 1.688028480254468E-4 0.01387135328728526 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.1558026058093276E-5 3.1558026058093276E-5 0.0 2 6.311605211618655E-5 3.1558026058093276E-5 0.0 3 6.311605211618655E-5 3.1558026058093276E-5 0.0 4 9.467407817427983E-5 3.1558026058093276E-5 3.1558026058093276E-5 5 9.467407817427983E-5 3.1558026058093276E-5 6.311605211618655E-5 6 9.467407817427983E-5 3.1558026058093276E-5 6.311605211618655E-5 7 1.577901302904664E-4 3.1558026058093276E-5 9.467407817427983E-5 8 1.577901302904664E-4 3.1558026058093276E-5 9.467407817427983E-5 9 1.577901302904664E-4 3.1558026058093276E-5 9.467407817427983E-5 10-11 2.524642084647462E-4 6.311605211618655E-5 1.262321042323731E-4 12-13 2.840222345228395E-4 6.311605211618655E-5 1.262321042323731E-4 14-15 3.155802605809328E-4 9.467407817427983E-5 1.262321042323731E-4 16-17 3.155802605809328E-4 1.262321042323731E-4 1.262321042323731E-4 18-19 3.4713828663902603E-4 1.262321042323731E-4 1.577901302904664E-4 20-21 3.786963126971193E-4 1.4201111726141975E-4 1.577901302904664E-4 22-23 3.786963126971193E-4 1.7356914331951304E-4 1.577901302904664E-4 24-25 3.786963126971193E-4 1.8934815634855966E-4 1.577901302904664E-4 26-27 3.786963126971193E-4 1.8934815634855966E-4 1.577901302904664E-4 28-29 4.891494039004458E-4 2.2090618240665295E-4 1.577901302904664E-4 30-31 5.522654560166324E-4 2.2090618240665295E-4 1.8934815634855966E-4 32-33 5.838234820747256E-4 2.524642084647462E-4 1.8934815634855966E-4 34-35 6.627185472199589E-4 2.6824322149379286E-4 1.8934815634855966E-4 36-37 9.15182755684705E-4 2.840222345228395E-4 1.8934815634855966E-4 38-39 0.0011045309120332647 2.840222345228395E-4 2.051271693776063E-4 40-41 0.001435890185643244 3.155802605809328E-4 2.2090618240665295E-4 42-43 0.0019408186025727365 3.155802605809328E-4 2.2090618240665295E-4 44-45 0.0027455482670541154 3.155802605809328E-4 2.2090618240665295E-4 46-47 0.0037080680618259603 3.155802605809328E-4 2.2090618240665295E-4 48-49 0.005270190351701577 3.155802605809328E-4 2.2090618240665295E-4 50-51 0.006690301524315775 3.155802605809328E-4 2.2090618240665295E-4 52-53 0.00900981643958563 3.155802605809328E-4 2.2090618240665295E-4 54-55 0.01159757457634928 3.155802605809328E-4 2.2090618240665295E-4 56-57 0.014990062377594307 3.155802605809328E-4 2.2090618240665295E-4 58-59 0.019786882338424485 3.155802605809328E-4 2.2090618240665295E-4 60-61 0.02507285170315511 3.3135927360997944E-4 2.2090618240665295E-4 62-63 0.03165270013626756 3.4713828663902603E-4 2.524642084647462E-4 64-65 0.03957376467684897 3.4713828663902603E-4 2.9980124755188615E-4 66-67 0.048536244077347454 3.4713828663902603E-4 3.155802605809328E-4 68-69 0.059455321093447734 3.4713828663902603E-4 3.4713828663902603E-4 70-71 0.07288326118116642 3.4713828663902603E-4 3.4713828663902603E-4 72-73 0.0891514236141135 3.4713828663902603E-4 3.6291729966807267E-4 74-75 0.10529335394282821 3.4713828663902603E-4 3.786963126971193E-4 76-77 0.1246384239164394 3.4713828663902603E-4 3.786963126971193E-4 78-79 0.14887498792905504 3.4713828663902603E-4 4.102543387552126E-4 80-81 0.17713520026407756 3.786963126971193E-4 4.102543387552126E-4 82-83 0.20839342507461894 4.102543387552126E-4 4.418123648133059E-4 84-85 0.24279167347794062 4.102543387552126E-4 4.418123648133059E-4 86-87 0.28298081966292243 4.575913778423525E-4 4.575913778423525E-4 88-89 0.3251581214895641 5.049284169294924E-4 4.7337039087139913E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTACT 985 1.6470221E-7 8.246562 1 GTAAACT 1420 2.7976057E-9 7.4027753 1 GTATCTA 975 8.242802E-5 6.860941 1 GTTGCAA 1905 7.6397555E-11 6.7721887 1 GTCTTAC 840 0.0059273895 6.257108 1 GTTAGCT 845 0.006304394 6.2200837 1 GTTCTTA 1485 2.4884193E-6 6.1134644 1 GTCATTT 2615 3.6379788E-12 6.029794 1 GTTTCCT 2465 2.7284841E-11 6.009039 1 GGACAAT 1285 7.383396E-5 5.949454 1 GGGTCAA 1140 6.241496E-4 5.86791 1 GTTAATT 2280 8.913048E-10 5.86791 1 GCTAAAG 1650 2.4075198E-6 5.791703 1 GTCTACA 1075 0.0019784505 5.778234 1 GTTTTCT 4225 0.0 5.7677135 1 GTATAGA 1080 0.0020909489 5.751483 1 GTTATCC 1330 1.2137011E-4 5.7481565 1 GTGCATT 1755 1.1822212E-6 5.717451 1 GTTTCTG 3035 1.8189894E-12 5.667663 1 GTCTCTT 1690 3.6779766E-6 5.6546216 1 >>END_MODULE