FastQCFastQC Report
Thu 11 Sep 2014
LONLib36_CCAACA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib36_CCAACA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3168766
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACT9851.6470221E-78.2465621
GTAAACT14202.7976057E-97.40277531
GTATCTA9758.242802E-56.8609411
GTTGCAA19057.6397555E-116.77218871
GTCTTAC8400.00592738956.2571081
GTTAGCT8450.0063043946.22008371
GTTCTTA14852.4884193E-66.11346441
GTCATTT26153.6379788E-126.0297941
GTTTCCT24652.7284841E-116.0090391
GGACAAT12857.383396E-55.9494541
GGGTCAA11406.241496E-45.867911
GTTAATT22808.913048E-105.867911
GCTAAAG16502.4075198E-65.7917031
GTCTACA10750.00197845055.7782341
GTTTTCT42250.05.76771351
GTATAGA10800.00209094895.7514831
GTTATCC13301.2137011E-45.74815651
GTGCATT17551.1822212E-65.7174511
GTTTCTG30351.8189894E-125.6676631
GTCTCTT16903.6779766E-65.65462161
Produced by FastQC (version 0.11.2)