##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib35_CATTTT_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 622854 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.79608704447591 27.0 22.0 33.0 2.0 33.0 2 24.78837255600831 27.0 15.0 33.0 2.0 33.0 3 25.679555722528875 33.0 22.0 33.0 6.0 33.0 4 27.79703269144936 33.0 22.0 37.0 2.0 37.0 5 27.868152087005942 33.0 15.0 37.0 6.0 37.0 6 28.91247065925562 33.0 22.0 37.0 15.0 37.0 7 28.57802149460387 33.0 27.0 37.0 6.0 37.0 8 28.3384308361189 33.0 22.0 37.0 2.0 37.0 9 29.072061831504655 33.0 27.0 37.0 15.0 37.0 10-11 28.661504140617225 33.0 24.5 37.0 10.5 37.0 12-13 28.965901318768122 33.0 27.0 37.0 10.5 37.0 14-15 29.26432117318023 33.0 24.5 37.0 6.0 40.0 16-17 30.110799641649567 33.0 27.0 37.0 15.0 40.0 18-19 30.401559595025482 33.0 27.0 37.0 15.0 40.0 20-21 30.38807409119954 33.0 27.0 37.0 15.0 40.0 22-23 30.366321802541208 33.0 27.0 37.0 15.0 40.0 24-25 29.922340548507353 33.0 27.0 37.0 10.5 40.0 26-27 29.363067428321884 33.0 27.0 37.0 6.0 40.0 28-29 29.527687066310882 33.0 27.0 37.0 15.0 40.0 30-31 29.386637960099797 33.0 24.5 37.0 10.5 40.0 32-33 29.171785843873526 33.0 22.0 37.0 6.0 40.0 34-35 29.180051825949583 33.0 22.0 37.0 10.5 40.0 36-37 29.40483564366609 33.0 27.0 37.0 6.0 40.0 38-39 29.412899331143414 33.0 24.5 37.0 15.0 40.0 40-41 29.454287842736818 33.0 24.5 37.0 15.0 40.0 42-43 29.31495743785863 33.0 24.5 37.0 15.0 40.0 44-45 29.01207746919824 33.0 22.0 37.0 10.5 40.0 46-47 28.608975779235585 33.0 22.0 37.0 6.0 40.0 48-49 28.399952476824424 33.0 22.0 37.0 10.5 40.0 50-51 27.58462978482919 30.0 22.0 37.0 10.5 38.5 52-53 27.285550064702164 33.0 22.0 37.0 6.0 37.0 54-55 28.180918000044954 33.0 22.0 37.0 6.0 40.0 56-57 28.072967340660895 33.0 22.0 37.0 6.0 40.0 58-59 28.23766002947721 33.0 22.0 37.0 6.0 40.0 60-61 27.988032508420915 33.0 22.0 37.0 6.0 40.0 62-63 27.824389824902788 33.0 22.0 37.0 6.0 40.0 64-65 27.7837848035013 33.0 22.0 37.0 6.0 40.0 66-67 27.652122327222752 33.0 22.0 37.0 6.0 38.5 68-69 27.33367771580499 33.0 22.0 37.0 6.0 37.0 70-71 27.23290289538158 30.0 22.0 37.0 6.0 37.0 72-73 26.980113317085546 27.0 22.0 37.0 6.0 37.0 74-75 26.9150956725011 27.0 22.0 33.0 6.0 37.0 76-77 26.538645493165333 27.0 22.0 33.0 6.0 37.0 78-79 25.975539211436388 27.0 22.0 33.0 6.0 37.0 80-81 25.853078731131212 27.0 22.0 33.0 6.0 37.0 82-83 25.449211211616205 27.0 18.5 33.0 6.0 37.0 84-85 25.530738343175127 27.0 18.5 33.0 6.0 37.0 86-87 25.232085689423204 27.0 15.0 33.0 6.0 37.0 88-89 24.884355723813286 27.0 15.0 33.0 6.0 37.0 90-91 24.761063587935535 27.0 15.0 33.0 6.0 37.0 92-93 24.643088910081655 27.0 15.0 33.0 6.0 37.0 94-95 24.42345076053136 27.0 15.0 33.0 6.0 37.0 96-97 24.30189498662608 27.0 15.0 33.0 6.0 37.0 98-99 24.068699887935214 27.0 15.0 33.0 6.0 37.0 100-101 23.57615749437268 27.0 15.0 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.370863578374273 2306 2 8.588388284420677 2306 3 7.514391783502969 2306 4 9.097319395045655 2306 5 8.773458111882949 2306 6 7.79211079308811 2306 7 8.15128183597352 2306 8 8.56455270982537 2306 9 7.322986451226271 2306 10-11 7.730645366768286 2306 12-13 7.502801608634957 2306 14-15 8.103353399042227 2306 16-17 6.657270272021645 2306 18-19 6.243296477092649 2306 20-21 6.274622597631161 2306 22-23 5.871021881728474 2306 24-25 5.746127519294628 2306 26-27 6.527977846942651 2306 28-29 5.415145787240114 2306 30-31 5.469308926827907 2306 32-33 5.256123014680664 2306 34-35 4.748223271726907 2306 36-37 4.769003316729162 2306 38-39 4.409115706370166 2306 40-41 3.7007064698145875 2306 42-43 3.4276527570689908 2306 44-45 3.2890272269148504 2306 46-47 3.6751746325498864 2306 48-49 3.371621231376899 2306 50-51 3.0976225747955866 2306 52-53 3.601381934164092 2306 54-55 2.8745376960323235 2306 56-57 3.0277706397414725 2306 58-59 2.795255831715302 2306 60-61 2.698621630843725 2306 62-63 2.5790440868937416 2306 64-65 1.9580898244218972 2306 66-67 1.6475992512907283 2306 68-69 1.9835869839640523 2306 70-71 2.2109634328604564 2306 72-73 1.2083944184558248 2306 74-75 0.920096497421909 2306 76-77 1.4534383214301378 2306 78-79 1.412489241559804 2306 80-81 1.127551783083181 2306 82-83 1.340177333640387 2306 84-85 1.1246289104626328 2306 86-87 1.0107175664431018 2306 88-89 0.8170449785317722 2306 90-91 0.7503454401706726 2306 92-93 0.883106789213155 2306 94-95 0.9506652472616537 2306 96-97 0.6240575491629912 2306 98-99 0.3835087518346363 2306 100-101 0.3326105337263243 2307 1 8.374132312024145 2307 2 8.483592476941855 2307 3 7.397750691491044 2307 4 9.322663964005084 2307 5 8.887217965013821 2307 6 7.650966254094936 2307 7 8.227124034326067 2307 8 8.46302735819539 2307 9 7.427122656498611 2307 10-11 7.77300026719935 2307 12-13 7.172925589738991 2307 14-15 8.258892077047854 2307 16-17 6.891759717525236 2307 18-19 6.376886590292791 2307 20-21 6.423199286290309 2307 22-23 5.940757162524399 2307 24-25 6.207577817493377 2307 26-27 6.835130311495487 2307 28-29 6.04967622107926 2307 30-31 5.66188004933997 2307 32-33 5.837543415505255 2307 34-35 5.437144193516687 2307 36-37 5.377696460482831 2307 38-39 5.0253314152358755 2307 40-41 4.285092497269883 2307 42-43 4.195968773691661 2307 44-45 3.9969362534763277 2307 46-47 4.29713129169054 2307 48-49 4.173269548013508 2307 50-51 4.187328474877042 2307 52-53 4.661095739138833 2307 54-55 4.070824462827641 2307 56-57 4.580971088202087 2307 58-59 3.9708348780648457 2307 60-61 3.9541279315086655 2307 62-63 3.666596377160044 2307 64-65 3.4911930719725 2307 66-67 3.306717519860772 2307 68-69 3.406139696169099 2307 70-71 3.1871402544733627 2307 72-73 2.7760406718980732 2307 74-75 2.370134644129152 2307 76-77 2.5418637665755135 2307 78-79 2.9125641572151117 2307 80-81 2.438225283435372 2307 82-83 2.9003414936464473 2307 84-85 2.163977712587137 2307 86-87 1.7799885423960369 2307 88-89 2.2656554222907843 2307 90-91 1.9408370074234007 2307 92-93 1.7526054398702584 2307 94-95 1.9788404224523504 2307 96-97 1.8964207333711158 2307 98-99 1.6208579087321304 2307 100-101 1.0617336072314991 1101 1 8.093966742642419 1101 2 7.859245964699372 1101 3 7.275452252967725 1101 4 8.840882340942574 1101 5 8.637828652913814 1101 6 7.34830576899159 1101 7 7.1342690253065015 1101 8 8.104302506702282 1101 9 7.474412764848317 1101 10-11 7.573209670529117 1101 12-13 7.319272664986311 1101 14-15 7.767515530220791 1101 16-17 6.992035762243912 1101 18-19 5.904882005824831 1101 20-21 5.780228516582273 1101 22-23 5.331657740466106 1101 24-25 6.09365637390302 1101 26-27 6.569075768965643 1101 28-29 5.714812721027563 1101 30-31 5.555079180703942 1101 32-33 5.675397447883778 1101 34-35 5.326597619228771 1101 36-37 5.348644261891501 1101 38-39 5.104726299934782 1101 40-41 4.370546865153326 1101 42-43 4.136448821489623 1101 44-45 4.103172106543553 1101 46-47 4.2604966243804405 1101 48-49 4.121973775145747 1101 50-51 3.584412339618005 1101 52-53 4.417596721524827 1101 54-55 4.194375587113829 1101 56-57 4.456859257057577 1101 58-59 3.874725930883887 1101 60-61 3.9446131497592134 1101 62-63 3.66309224654292 1101 64-65 3.4176758428754894 1101 66-67 3.154372432365225 1101 68-69 3.284748959322158 1101 70-71 3.2931072440060696 1101 72-73 2.8485485137511617 1101 74-75 2.2686501497419655 1101 76-77 2.3643012328239514 1101 78-79 2.929184289808493 1101 80-81 2.808540853391591 1101 82-83 2.766645941384663 1101 84-85 2.09593513103761 1101 86-87 2.247796155600227 1101 88-89 2.3753382437493116 1101 90-91 2.105906004240701 1101 92-93 1.9938828863560758 1101 94-95 2.1447412876414162 1101 96-97 2.0611453205966512 1101 98-99 1.866998740370704 1101 100-101 1.5567866330952498 2201 1 7.217316873140568 2201 2 7.892692471755311 2201 3 6.370879733280017 2201 4 8.549618373120818 2201 5 4.163413069965571 2201 6 5.597580402636385 2201 7 6.709235412145958 2201 8 7.6283638803789415 2201 9 6.423838234020245 2201 10-11 4.359126321189525 2201 12-13 6.566711003196847 2201 14-15 7.0613739256019485 2201 16-17 6.051831315138035 2201 18-19 2.040792442287483 2201 20-21 5.13362231594277 2201 22-23 5.031537411000635 2201 24-25 5.462080057905414 2201 26-27 6.299305863760406 2201 28-29 5.0688731343282 2201 30-31 5.266540421057812 2201 32-33 5.543825368839176 2201 34-35 5.067248641181834 2201 36-37 5.009637177706061 2201 38-39 4.3196864160837904 2201 40-41 4.277710705301569 2201 42-43 4.154552474308041 2201 44-45 3.834131522182993 2201 46-47 4.159754027749667 2201 48-49 4.033201983084105 2201 50-51 3.6926003925403705 2201 52-53 4.457972623995818 2201 54-55 3.8875244340976813 2201 56-57 4.395730476283173 2201 58-59 3.9533168897910933 2201 60-61 4.0325146716727325 2201 62-63 3.868211898095904 2201 64-65 3.6692217657968413 2201 66-67 3.1434518272153475 2201 68-69 3.4500070744994886 2201 70-71 3.2593768610985023 2201 72-73 2.599484315439163 2201 74-75 2.44185113949419 2201 76-77 2.6821484087535374 2201 78-79 2.952555322608159 2201 80-81 2.550721415381041 2201 82-83 2.933181639126083 2201 84-85 2.2945810008558176 2201 86-87 2.1887691859820038 2201 88-89 2.451609329652122 2201 90-91 2.041051811916933 2201 92-93 2.3636513792436418 2201 94-95 2.4565228416427907 2201 96-97 2.3333325487254974 2201 98-99 2.308589518501247 2201 100-101 1.7227842378044826 2304 1 1.2885849975218662 2304 2 4.688135172506492 2304 3 5.861631931498149 2304 4 4.3960488162583005 2304 5 6.875586976679603 2304 6 6.585073851655192 2304 7 7.179217360553345 2304 8 6.770017202454291 2304 9 6.0530723515302896 2304 10-11 6.809382173399385 2304 12-13 6.721009174745202 2304 14-15 7.0866745428499485 2304 16-17 5.673436451107282 2304 18-19 5.498495668281777 2304 20-21 5.12374889545713 2304 22-23 4.673778865609613 2304 24-25 5.012270036359968 2304 26-27 6.041858489975976 2304 28-29 4.9816468197646415 2304 30-31 5.064572579096591 2304 32-33 5.372132522510643 2304 34-35 4.975636080767231 2304 36-37 5.278502183820315 2304 38-39 4.5692857144009125 2304 40-41 4.261692100514988 2304 42-43 3.4245802795837648 2304 44-45 3.37431160137708 2304 46-47 4.19610942575817 2304 48-49 4.0617125811158985 2304 50-51 3.6478954050600265 2304 52-53 4.132701486163175 2304 54-55 3.8941674929691708 2304 56-57 4.646619590008211 2304 58-59 4.066861407347723 2304 60-61 3.903159361010644 2304 62-63 3.6400870800676053 2304 64-65 3.558017404299143 2304 66-67 3.397017380700472 2304 68-69 3.5380784656367403 2304 70-71 3.511333420814335 2304 72-73 3.0725797996294624 2304 74-75 2.5009908500863958 2304 76-77 2.819252438334857 2304 78-79 3.2829515113928807 2304 80-81 3.079601415963353 2304 82-83 3.3001275716946488 2304 84-85 2.7343894938389894 2304 86-87 2.5422350189756564 2304 88-89 2.602146510109815 2304 90-91 2.6963049709456364 2304 92-93 2.4950596946224337 2304 94-95 2.7479057537722973 2304 96-97 2.5812515203670294 2304 98-99 2.592981805812723 2304 100-101 2.0986566101331405 1102 1 7.0953645758115975 1102 2 7.315069638209991 1102 3 6.090147810711997 1102 4 6.173902519238908 1102 5 6.477170017158578 1102 6 5.610978446214606 1102 7 6.5871960147248885 1102 8 6.455493253476028 1102 9 5.155381020019163 1102 10-11 6.032041779022187 1102 12-13 5.866865285639388 1102 14-15 6.160784591008216 1102 16-17 5.356623357263732 1102 18-19 5.462765810153442 1102 20-21 5.8929788512035834 1102 22-23 5.402389109004126 1102 24-25 1.97412901071894 1102 26-27 5.5976252802922595 1102 28-29 5.254855527300762 1102 30-31 4.905189037511278 1102 32-33 4.788365602036873 1102 34-35 5.320929260514724 1102 36-37 5.068749320355522 1102 38-39 4.213086150428509 1102 40-41 4.297317832650872 1102 42-43 4.47468277860758 1102 44-45 -4.121541255579839 1102 46-47 1.457918777573866 1102 48-49 -0.797064914653987 1102 50-51 2.6369011645289824 1102 52-53 -0.16281214783396436 1102 54-55 2.5294963033297044 1102 56-57 -0.8371863777127331 1102 58-59 2.8548153315960647 1102 60-61 3.9502739321417124 1102 62-63 3.8792140486996516 1102 64-65 3.2596102065899117 1102 66-67 3.049539995564455 1102 68-69 3.8190949956380393 1102 70-71 3.30999930653606 1102 72-73 2.7809253352275363 1102 74-75 2.688574638970948 1102 76-77 2.9305549894216725 1102 78-79 -1.2532916553388667 1102 80-81 1.7341772652409766 1102 82-83 2.568520452079916 1102 84-85 2.5112645751874183 1102 86-87 2.456541767641543 1102 88-89 2.4371491132596255 1102 90-91 2.0902217352304255 1102 92-93 2.2980435550936065 1102 94-95 2.3896171620763447 1102 96-97 2.6798279228139883 1102 98-99 2.517922421457655 1102 100-101 2.3867219817327765 2202 1 -20.587376540939673 2202 2 -7.634775272242884 2202 3 -0.8333949068261717 2202 4 1.2423842751331087 2202 5 1.2241734243347793 2202 6 1.9955650131694398 2202 7 3.6724775106865266 2202 8 2.584190057406264 2202 9 2.79157929317207 2202 10-11 1.9043419159129087 2202 12-13 1.2778610191232822 2202 14-15 2.398395626068936 2202 16-17 1.3789635315819027 2202 18-19 1.0660122152185778 2202 20-21 0.9573396816384125 2202 22-23 1.1829795678454715 2202 24-25 2.0275567826671725 2202 26-27 3.475650059109885 2202 28-29 -0.7954185204565221 2202 30-31 0.6531183656814648 2202 32-33 1.301939710338555 2202 34-35 1.3320540725341203 2202 36-37 2.864407523234309 2202 38-39 2.2730819247488014 2202 40-41 1.3926459708838586 2202 42-43 0.35603661181396973 2202 44-45 0.47759576474781085 2202 46-47 -1.400785501193159 2202 48-49 0.4878799473278619 2202 50-51 1.3638707662675493 2202 52-53 1.696756797326298 2202 54-55 -0.9049834675603314 2202 56-57 0.7426225967077009 2202 58-59 1.9301917810663447 2202 60-61 1.696953755231327 2202 62-63 -2.394388059168815 2202 64-65 -0.22033335040261193 2202 66-67 0.2016520364434662 2202 68-69 1.319509573877923 2202 70-71 -2.9619533658512545 2202 72-73 -4.716666204890675 2202 74-75 -1.3406433617143918 2202 76-77 -0.47012422644841934 2202 78-79 1.396843371107142 2202 80-81 1.5067096369658515 2202 82-83 -1.0181033186112671 2202 84-85 -0.5694632182443087 2202 86-87 0.3264574138420677 2202 88-89 1.1537023257527252 2202 90-91 1.5174477185443145 2202 92-93 1.3387294823373423 2202 94-95 1.702517203445396 2202 96-97 1.2853105646805325 2202 98-99 1.4172074293497339 2202 100-101 0.9046331820921338 2305 1 5.592186951123818 2305 2 6.9714379808092986 2305 3 6.723124605490824 2305 4 7.928329036038523 2305 5 7.746099797192418 2305 6 5.957112923694265 2305 7 2.7143211456571272 2305 8 6.022047116984204 2305 9 6.310535721493665 2305 10-11 4.584855311372525 2305 12-13 5.4142249867110195 2305 14-15 5.586911111836521 2305 16-17 4.874092739263578 2305 18-19 4.546068928355034 2305 20-21 5.093544377752611 2305 22-23 5.17155053838129 2305 24-25 5.289109169449095 2305 26-27 6.210730590098077 2305 28-29 5.164529908897727 2305 30-31 5.5282401615141765 2305 32-33 5.489890459018579 2305 34-35 5.474117460498611 2305 36-37 4.931585211903339 2305 38-39 4.72594017105537 2305 40-41 4.291660049232927 2305 42-43 3.9618589731124594 2305 44-45 3.3492734450889223 2305 46-47 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42-43 -22.678801457726543 1209 44-45 -25.665111475546 1209 46-47 -18.863777413592956 1209 48-49 -18.906909867863696 1209 50-51 -20.969369770240863 1209 52-53 -23.626433129221443 1209 54-55 -20.361297577674474 1209 56-57 -24.450976563837944 1209 58-59 -24.694677054190738 1209 60-61 -24.43001371591243 1209 62-63 -24.545489843009317 1209 64-65 -24.387174903393166 1209 66-67 -12.06111644975479 1209 68-69 -24.07345999590172 1209 70-71 -23.861743502262588 1209 72-73 -13.594691916749397 1209 74-75 -14.441117339343219 1209 76-77 -11.977012606144473 1209 78-79 -12.95207269059561 1209 80-81 -22.897802430190495 1209 82-83 -14.362450921240985 1209 84-85 -11.769244074973152 1209 86-87 -11.984115713625076 1209 88-89 -9.693889596640574 1209 90-91 -11.714415855846617 1209 92-93 -16.536455219035336 1209 94-95 -21.611228861612318 1209 96-97 -16.981719437246785 1209 98-99 -8.656341192617417 1209 100-101 -4.730029287302756 1212 1 -20.587376540939673 1212 2 -20.36090328903197 1212 3 -21.30663729927108 1212 4 -23.277028106818623 1212 5 -23.483066869474246 1212 6 -24.509156917575577 1212 7 -24.05155187021589 1212 8 -23.700175105238017 1212 9 -24.58827380231586 1212 10-11 -24.30552167275446 1212 12-13 -24.56841390217787 1212 14-15 -24.873902380226973 1212 16-17 -25.92848662581579 1212 18-19 -20.492850485564375 1212 20-21 -26.237308796850566 1212 22-23 -26.381028826698078 1212 24-25 -22.707494920904992 1212 26-27 -25.043237663870176 1212 28-29 -19.888545487927665 1212 30-31 -14.77907058329657 1212 32-33 -25.071617477489358 1212 34-35 -21.10459896196781 1212 36-37 -25.449863200795072 1212 38-39 -25.70907967021447 1212 40-41 -20.270038668715785 1212 42-43 -16.421039378535895 1212 44-45 -13.227077287511811 1212 46-47 -17.070824334925593 1212 48-49 -19.600787418884103 1212 50-51 -11.647243483828863 1212 52-53 -5.066604069392383 1212 54-55 -3.7937064386547625 1212 56-57 -3.4787543416157227 1212 58-59 -2.6305744900881756 1212 60-61 -13.733432519331235 1212 62-63 -11.150190697710173 1212 64-65 -15.45127746749573 1212 66-67 -12.052395012461924 1212 68-69 -7.879015551457279 1212 70-71 -14.54550418602327 1212 72-73 -11.403648832849171 1212 74-75 -8.056327525981978 1212 76-77 -18.04517063858822 1212 78-79 -8.982379685188317 1212 80-81 -7.0559220883101545 1212 82-83 -4.4610903770233 1212 84-85 -3.5710058053063136 1212 86-87 -2.922455151964513 1212 88-89 -2.6228534898901863 1212 90-91 -3.527733490592823 1212 92-93 -2.672247997685254 1212 94-95 -3.0620835624670164 1212 96-97 -12.45176130014579 1212 98-99 -9.63410152752061 1212 100-101 -5.984302791576262 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 103.0 3 221.0 4 115.0 5 719.0 6 909.0 7 922.0 8 1948.0 9 3672.0 10 4780.0 11 6085.0 12 7809.0 13 9495.0 14 11012.0 15 11492.0 16 12197.0 17 13361.0 18 15001.0 19 15851.0 20 17379.0 21 18962.0 22 20635.0 23 21940.0 24 22624.0 25 23357.0 26 24305.0 27 25379.0 28 25398.0 29 25020.0 30 25954.0 31 27225.0 32 29255.0 33 32816.0 34 36649.0 35 39318.0 36 40492.0 37 37135.0 38 13292.0 39 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4051935462824 20.802706758507554 13.170654412929805 21.621445282280238 2 25.53254218464839 26.67440813070775 25.34823022535257 22.444819459291292 3 21.308993242628688 25.012974366931147 27.98896420858145 25.689068181858715 4 24.687313334984484 25.10726409184283 25.877962965660924 24.327459607511763 5 23.172797012838778 27.026730491872904 25.697903855172232 24.10256864011609 6 22.679713464322365 27.049620421783782 25.670293671884025 24.600372442009828 7 24.86150214232605 26.008890251926132 26.381546219146674 22.748061386601144 8 23.097733705979394 26.09190425238779 26.844114899997496 23.966247141635325 9 23.450861874076807 25.866251013494562 26.815578835003933 23.867308277424694 10-11 24.496086736140345 25.97977645856064 25.55752197183825 23.96661483346077 12-13 24.077841956056524 26.056088549600165 25.69061500990143 24.17545448444189 14-15 23.876493024233074 26.35588783553704 25.902760352951493 23.864858787278393 16-17 24.434753455064346 26.2489925481595 25.4913392172925 23.824914779483656 18-19 24.333027251258546 26.345786477476423 25.303531390831974 24.01765488043306 20-21 24.07803320381959 26.585438035259678 25.52293901966912 23.813589741251604 22-23 24.233414434180524 26.20116108088731 25.47183432856133 24.09359015637084 24-25 24.124569030130417 26.20538316760772 25.728693016206133 23.94135478605573 26-27 23.93849489838799 26.351044695836347 25.80096148997327 23.90949891580239 28-29 24.31877720334307 26.243374134090768 25.54950242148068 23.88834624108548 30-31 24.240658190764517 26.163313049146474 25.53555267490693 24.060476085182078 32-33 24.006306977715845 26.37565148476767 25.695628637724315 23.92241289979217 34-35 24.360432086974843 26.189677885833223 25.5353335295328 23.914556497659134 36-37 24.23874083170157 26.143174803358665 25.47376456141515 24.14431980352461 38-39 24.000355110744053 26.287398618325998 25.879754289428288 23.832491981501665 40-41 24.443386446285288 26.131344874540556 25.457981404508807 23.96728727466535 42-43 24.211681165597813 26.237495075530067 25.477996499589896 24.072827259282224 44-45 24.035681964672868 26.12505127186786 25.886433626532458 23.952833136926813 46-47 24.39504093597732 26.14478623137358 25.39797848835363 24.062194344295474 48-49 24.13417439123512 26.081066677948755 25.684674884690832 24.100084046125296 50-51 24.179066250564855 26.176322327734013 25.582186361626842 24.06242506007429 52-53 24.33477642509086 26.15641555442641 25.498500771231935 24.010307249250797 54-55 24.15032893660197 26.028213268746114 25.646335875364123 24.175121919287797 56-57 23.95402761550369 26.20731918848776 25.816388646159588 24.022264549848963 58-59 24.353107952914154 26.079105003523605 25.484169864014827 24.08361717954741 60-61 24.238102374800853 25.848073345475044 25.6321453355966 24.281678944127506 62-63 24.17836450738218 26.14395714547647 25.658192850202216 24.019485496939136 64-65 24.423747654940627 26.014118741527493 25.37836064187335 24.183772961658534 66-67 24.39987276511439 25.892137755983512 25.467241053595274 24.240748425306823 68-69 24.162289469637496 26.064731257638172 25.701902765525226 24.07107650719911 70-71 24.599150813702153 25.8988843784597 25.339492029247047 24.162472778591095 72-73 24.44200024263479 25.965537825719124 25.46596725715833 24.126494674487756 74-75 24.290866485696792 26.040616969059936 25.51586861522686 24.15264793001641 76-77 24.628079709706586 25.97146910631859 25.27243236878039 24.12801881519443 78-79 24.437495443093347 25.85641490938844 25.472014517291942 24.234075130226266 80-81 24.453870352432876 25.97275128888288 25.479179398486433 24.094198960197815 82-83 24.702334357160037 25.865639289121024 25.260704584301074 24.171321769417872 84-85 24.67489591608704 25.758268275030545 25.17750293394939 24.38933287493303 86-87 24.522454214102506 26.00611657163428 25.353800116995906 24.117629097267308 88-89 24.836545645903797 25.874153403658745 25.20322329254674 24.08607765789072 90-91 24.842502761876066 25.80632720699006 25.061685246560213 24.289484784573666 92-93 24.763278707507773 25.934682212470577 25.152486709431297 24.14955237059035 94-95 25.170634770216683 25.78155684720657 24.88231180978911 24.165496572787635 96-97 25.141209018358584 25.765196137291674 24.85149783502338 24.242097009326365 98-99 25.02795423217436 25.902487926663518 24.955016178110228 24.114541663051895 100-101 25.65200319906796 25.690945575614993 24.429341310620163 24.227709914696884 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 45.0 1 39.5 2 35.5 3 44.5 4 58.5 5 66.5 6 62.0 7 83.0 8 114.0 9 140.5 10 194.5 11 262.5 12 351.5 13 458.5 14 580.0 15 737.0 16 919.5 17 1163.5 18 1518.0 19 1896.0 20 2366.5 21 2974.0 22 3608.5 23 4316.0 24 5157.0 25 6026.0 26 7062.5 27 8322.5 28 9381.5 29 10374.5 30 11426.0 31 12383.0 32 13280.5 33 14188.5 34 14980.5 35 15553.0 36 16045.0 37 16401.5 38 16462.0 39 16449.0 40 16175.0 41 15732.5 42 15286.0 43 14703.5 44 14099.0 45 13534.5 46 13160.5 47 12852.5 48 12384.5 49 12019.5 50 11637.0 51 11135.0 52 10685.5 53 10303.5 54 10182.5 55 9971.5 56 9911.5 57 10064.0 58 10096.0 59 10177.5 60 10224.0 61 10205.0 62 10277.5 63 10338.5 64 10331.0 65 10358.0 66 10338.5 67 10061.5 68 9585.5 69 9181.0 70 8727.0 71 8103.0 72 7325.5 73 6539.0 74 5730.0 75 4840.0 76 4076.5 77 3350.5 78 2729.0 79 2255.0 80 1784.5 81 1387.0 82 1139.5 83 910.5 84 728.5 85 603.0 86 468.0 87 376.0 88 305.0 89 256.0 90 202.0 91 134.5 92 99.5 93 77.5 94 66.0 95 52.5 96 34.5 97 19.0 98 8.5 99 5.5 100 3.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.874680101596843 2 11.236501652072556 3 9.97553198662929 4 11.839371666554282 5 9.274886249426029 6 5.507871828711062 7 9.956586936906563 8 10.270143564944593 9 5.5459224794253545 10-11 6.55996429339781 12-13 5.507309899270134 14-15 7.540691719086656 16-17 2.7881333346177435 18-19 3.222986446261885 20-21 3.162940271716967 22-23 3.261117372610596 24-25 3.6066237031471258 26-27 4.486444656372119 28-29 1.7887819617438436 30-31 2.340917775273178 32-33 3.1648668869430074 34-35 2.07600818169266 36-37 3.248594373641335 38-39 1.4386999200454682 40-41 1.1938592350695347 42-43 0.5616083383906982 44-45 1.3617958622726996 46-47 1.4301104271627059 48-49 1.3342613196672093 50-51 1.5835171645361512 52-53 2.5220196065209506 54-55 1.8935416646597758 56-57 1.8861563062932887 58-59 1.5807877929659278 60-61 1.4436770093794051 62-63 0.6148310840100569 64-65 1.2840087725213292 66-67 0.5662643251869619 68-69 0.8138343817331188 70-71 1.0245579220812584 72-73 0.08340638416065402 74-75 0.10451887601267712 76-77 0.47033494205704707 78-79 0.9085596303467586 80-81 0.30031114835900546 82-83 0.5270898155908126 84-85 0.06414023190025271 86-87 0.09793627399037334 88-89 0.0817205958378689 90-91 0.08693851207506093 92-93 0.17315454344035683 94-95 0.46391289130358 96-97 0.386687730993138 98-99 0.06550491768536447 100-101 0.22846445555459224 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 622854.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.71893141978909 #Duplication Level Percentage of deduplicated Percentage of total 1 99.72660395221847 99.4463038023974 2 0.26493129319674147 0.5283733091448379 3 0.008464754584786814 0.025322888457771045 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.6055126883667763E-4 0.0 0.0 2 1.6055126883667763E-4 0.0 0.0 3 1.6055126883667763E-4 0.0 0.0 4 1.6055126883667763E-4 0.0 1.6055126883667763E-4 5 1.6055126883667763E-4 0.0 1.6055126883667763E-4 6 1.6055126883667763E-4 0.0 1.6055126883667763E-4 7 1.6055126883667763E-4 0.0 1.6055126883667763E-4 8 1.6055126883667763E-4 0.0 1.6055126883667763E-4 9 1.6055126883667763E-4 0.0 1.6055126883667763E-4 10-11 3.2110253767335525E-4 0.0 1.6055126883667763E-4 12-13 3.2110253767335525E-4 0.0 1.6055126883667763E-4 14-15 3.2110253767335525E-4 0.0 1.6055126883667763E-4 16-17 3.2110253767335525E-4 0.0 1.6055126883667763E-4 18-19 3.2110253767335525E-4 0.0 1.6055126883667763E-4 20-21 3.2110253767335525E-4 0.0 1.6055126883667763E-4 22-23 3.2110253767335525E-4 0.0 1.6055126883667763E-4 24-25 3.2110253767335525E-4 0.0 1.6055126883667763E-4 26-27 3.2110253767335525E-4 1.6055126883667763E-4 1.6055126883667763E-4 28-29 5.619294409283717E-4 1.6055126883667763E-4 1.6055126883667763E-4 30-31 6.422050753467105E-4 1.6055126883667763E-4 1.6055126883667763E-4 32-33 0.0011238588818567434 1.6055126883667763E-4 1.6055126883667763E-4 34-35 0.0016857883227851152 1.6055126883667763E-4 1.6055126883667763E-4 36-37 0.0020871664948768093 1.6055126883667763E-4 1.6055126883667763E-4 38-39 0.003291301011151891 1.6055126883667763E-4 1.6055126883667763E-4 40-41 0.005057364968355345 1.6055126883667763E-4 1.6055126883667763E-4 42-43 0.007064255828813815 1.6055126883667763E-4 1.6055126883667763E-4 44-45 0.009552800495782318 1.6055126883667763E-4 1.6055126883667763E-4 46-47 0.014449614195300985 1.6055126883667763E-4 1.6055126883667763E-4 48-49 0.019506979163656332 1.6055126883667763E-4 1.6055126883667763E-4 50-51 0.024885446669685028 1.6055126883667763E-4 1.6055126883667763E-4 52-53 0.03243135630500888 1.6055126883667763E-4 1.6055126883667763E-4 54-55 0.04254608624171957 1.6055126883667763E-4 1.6055126883667763E-4 56-57 0.05619294409283716 1.6055126883667763E-4 1.6055126883667763E-4 58-59 0.07441551310580008 1.6055126883667763E-4 1.6055126883667763E-4 60-61 0.09560828059224152 1.6055126883667763E-4 1.6055126883667763E-4 62-63 0.12209923995029333 1.6055126883667763E-4 1.6055126883667763E-4 64-65 0.15003516072787523 1.6055126883667763E-4 1.6055126883667763E-4 66-67 0.18294817083939413 1.6055126883667763E-4 1.6055126883667763E-4 68-69 0.2256548083499504 1.6055126883667763E-4 1.6055126883667763E-4 70-71 0.273900464635372 1.6055126883667763E-4 1.6055126883667763E-4 72-73 0.3251163193942722 1.6055126883667763E-4 1.6055126883667763E-4 74-75 0.38508221830477124 1.6055126883667763E-4 1.6055126883667763E-4 76-77 0.45307568065710424 1.6055126883667763E-4 1.6055126883667763E-4 78-79 0.5298994627954545 1.6055126883667763E-4 1.6055126883667763E-4 80-81 0.6269527048072261 1.6055126883667763E-4 1.6055126883667763E-4 82-83 0.7286619336152613 1.6055126883667763E-4 1.6055126883667763E-4 84-85 0.8421716806827924 1.6055126883667763E-4 1.6055126883667763E-4 86-87 0.9637892668265757 1.6055126883667763E-4 1.6055126883667763E-4 88-89 1.1108542290809724 1.6055126883667763E-4 1.6055126883667763E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGG 450 9.3459676E-9 9.064131 92-93 TAGGGGA 245 0.0036982088 8.324202 94-95 CGTGTAG 465 1.3479766E-6 7.7971015 94-95 GTCGTGT 535 1.8691935E-7 7.5956926 88-89 GTGTAGG 490 0.0012292698 6.0119233 92-93 TCGTGTA 540 6.135947E-4 5.8748994 92-93 GAGCGTC 980 1.5895785E-7 5.4995046 84-85 AGAGCGT 950 4.1278508E-7 5.4861846 92-93 AGCGTCG 1040 4.51706E-7 5.229306 94-95 GCGTCGT 1030 2.3490156E-6 5.0256257 86-87 >>END_MODULE