FastQCFastQC Report
Thu 11 Sep 2014
LONLib35_CATTTT_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib35_CATTTT_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622854
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGG4509.3459676E-99.06413192-93
TAGGGGA2450.00369820888.32420294-95
CGTGTAG4651.3479766E-67.797101594-95
GTCGTGT5351.8691935E-77.595692688-89
GTGTAGG4900.00122926986.011923392-93
TCGTGTA5406.135947E-45.874899492-93
GAGCGTC9801.5895785E-75.499504684-85
AGAGCGT9504.1278508E-75.486184692-93
AGCGTCG10404.51706E-75.22930694-95
GCGTCGT10302.3490156E-65.025625786-87
Produced by FastQC (version 0.11.2)