##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib35_CATTTT_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 622854 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23586908007334 33.0 33.0 33.0 27.0 33.0 2 31.612721440337545 33.0 33.0 33.0 27.0 33.0 3 31.795817318344266 33.0 33.0 33.0 27.0 33.0 4 35.618677892411384 37.0 37.0 37.0 33.0 37.0 5 35.13490480915271 37.0 37.0 37.0 33.0 37.0 6 34.92325970452144 37.0 33.0 37.0 33.0 37.0 7 34.73238993407765 37.0 33.0 37.0 27.0 37.0 8 34.76479078564158 37.0 33.0 37.0 27.0 37.0 9 34.665979507236045 37.0 33.0 37.0 27.0 37.0 10-11 34.50889293478086 37.0 33.0 37.0 27.0 37.0 12-13 34.51379617053114 37.0 33.0 37.0 27.0 37.0 14-15 35.4265092622027 37.0 33.0 40.0 27.0 40.0 16-17 35.31982856335513 37.0 33.0 40.0 27.0 40.0 18-19 35.51830123913469 37.0 33.0 40.0 27.0 40.0 20-21 35.17321635567886 37.0 33.0 40.0 27.0 40.0 22-23 35.19078949480938 37.0 33.0 40.0 27.0 40.0 24-25 35.010461520677396 37.0 33.0 40.0 27.0 40.0 26-27 34.58589492882763 37.0 33.0 40.0 27.0 40.0 28-29 34.17413390618026 37.0 33.0 40.0 22.0 40.0 30-31 34.0460051633288 37.0 33.0 38.5 22.0 40.0 32-33 34.00036926791832 37.0 33.0 37.0 22.0 40.0 34-35 33.81017782658536 37.0 33.0 37.0 22.0 40.0 36-37 33.44412173639408 37.0 33.0 37.0 22.0 40.0 38-39 33.309467387220764 37.0 33.0 37.0 22.0 40.0 40-41 32.97175822905528 37.0 33.0 37.0 22.0 40.0 42-43 32.53665626294445 37.0 33.0 37.0 22.0 40.0 44-45 32.365305513009474 37.0 33.0 37.0 18.5 40.0 46-47 32.196772437842576 37.0 30.0 37.0 18.5 40.0 48-49 32.17949952958478 37.0 30.0 37.0 22.0 40.0 50-51 32.499652406502975 37.0 33.0 37.0 22.0 40.0 52-53 32.33853760271268 37.0 33.0 37.0 22.0 40.0 54-55 31.953241048464008 33.0 27.0 37.0 18.5 40.0 56-57 31.991033211635468 33.0 27.0 37.0 22.0 40.0 58-59 31.70466192719321 33.0 27.0 37.0 15.0 40.0 60-61 31.600375850520344 33.0 27.0 37.0 18.5 40.0 62-63 31.5648169554984 33.0 27.0 37.0 18.5 40.0 64-65 31.242219685512175 33.0 27.0 37.0 15.0 40.0 66-67 30.879644346829274 33.0 27.0 37.0 15.0 40.0 68-69 30.697320720425655 33.0 27.0 37.0 15.0 38.5 70-71 30.557731025248295 33.0 27.0 37.0 15.0 37.0 72-73 30.32792275557354 33.0 27.0 37.0 15.0 37.0 74-75 29.968176330247537 33.0 27.0 37.0 15.0 37.0 76-77 28.875506137875007 33.0 27.0 35.0 15.0 37.0 78-79 29.827880209487297 33.0 27.0 37.0 15.0 37.0 80-81 29.555925626230223 33.0 27.0 37.0 15.0 37.0 82-83 29.526022149653052 33.0 27.0 37.0 15.0 37.0 84-85 29.405900098578478 33.0 27.0 37.0 15.0 37.0 86-87 29.21451335304903 33.0 27.0 37.0 15.0 37.0 88-89 29.1053986969659 33.0 27.0 35.0 15.0 37.0 90-91 29.004351742141818 33.0 27.0 33.0 15.0 37.0 92-93 28.779356799506786 33.0 27.0 33.0 15.0 37.0 94-95 28.688137187848195 33.0 27.0 33.0 15.0 37.0 96-97 28.549847957948412 33.0 27.0 33.0 15.0 37.0 98-99 28.259781586053876 33.0 27.0 33.0 15.0 37.0 100-101 27.450971656279002 33.0 22.0 33.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.2179058476271543 2306 2 0.5989865127565182 2306 3 0.6044743575923945 2306 4 0.3551363861460928 2306 5 0.5326320232360402 2306 6 0.6386575351595312 2306 7 0.6653525417534425 2306 8 0.7643945423499829 2306 9 0.9458299248936157 2306 10-11 1.2391634744859417 2306 12-13 1.437389463653247 2306 14-15 1.6120548800466494 2306 16-17 1.4765852097704553 2306 18-19 1.4202602669951148 2306 20-21 1.5592925013775343 2306 22-23 1.2464315409202342 2306 24-25 1.2126107508425221 2306 26-27 1.1865411267052153 2306 28-29 1.6150777203150426 2306 30-31 1.5612974524156868 2306 32-33 1.1205751332454312 2306 34-35 1.0346090898500364 2306 36-37 1.2070745531457305 2306 38-39 1.1740286303907226 2306 40-41 1.1991691972830338 2306 42-43 1.3953611889269837 2306 44-45 1.2316565003525923 2306 46-47 1.1992087412674586 2306 48-49 1.2091946591407456 2306 50-51 1.3713730575076823 2306 52-53 1.5073538216848448 2306 54-55 1.9330939154177607 2306 56-57 1.385441854825217 2306 58-59 1.3139285326346801 2306 60-61 0.9838929140774582 2306 62-63 1.0404938178175414 2306 64-65 0.7094129534299896 2306 66-67 1.1012208769846588 2306 68-69 0.8971724195361439 2306 70-71 0.8901241908347082 2306 72-73 0.22550398342813338 2306 74-75 0.6774036732612814 2306 76-77 0.8183472400138072 2306 78-79 1.0864913048002158 2306 80-81 1.336556389841153 2306 82-83 1.243658739781626 2306 84-85 0.8475252803547235 2306 86-87 0.7279473739567415 2306 88-89 0.6713564782762553 2306 90-91 0.29996893621826715 2306 92-93 0.2039664864117725 2306 94-95 0.2338591359629163 2306 96-97 0.06862446388083043 2306 98-99 0.11009916224320904 2306 100-101 0.3410180234615936 2307 1 1.049857116285029 2307 2 0.7026870016888047 2307 3 0.4331103932281337 2307 4 0.35623514909057263 2307 5 0.8591712816227215 2307 6 0.7704953109051118 2307 7 0.9526842183313704 2307 8 0.8978907957061182 2307 9 1.009585730856898 2307 10-11 1.2242698977714497 2307 12-13 1.4487396159817436 2307 14-15 1.6030055855454606 2307 16-17 1.573732731612246 2307 18-19 1.3740045858912566 2307 20-21 1.6957354371756779 2307 22-23 1.4699514400682645 2307 24-25 1.4964937239779346 2307 26-27 1.7287660011138541 2307 28-29 1.7907092937332507 2307 30-31 1.6795608558597408 2307 32-33 1.4042497203051738 2307 34-35 1.4192424250777904 2307 36-37 1.5333262043039966 2307 38-39 1.2229373590117802 2307 40-41 1.4205578752025545 2307 42-43 1.4851816152401227 2307 44-45 1.29448748546762 2307 46-47 1.1213179872370809 2307 48-49 1.3346525487925724 2307 50-51 1.2981365462005883 2307 52-53 1.747608328249001 2307 54-55 2.0172930842845425 2307 56-57 1.5372846938357725 2307 58-59 1.7404388580786225 2307 60-61 1.2493578303010864 2307 62-63 1.2049457366055236 2307 64-65 1.1975659466979103 2307 66-67 1.5871384559862314 2307 68-69 1.2375426578712627 2307 70-71 1.4174253500468375 2307 72-73 1.2791987029938667 2307 74-75 1.5794010796296334 2307 76-77 1.2921250384579714 2307 78-79 1.1788872796767755 2307 80-81 1.8613612216425928 2307 82-83 1.824137958867727 2307 84-85 1.438187861965428 2307 86-87 1.5925153689554747 2307 88-89 1.3252866224415278 2307 90-91 0.8579183483456525 2307 92-93 0.9857742097852835 2307 94-95 1.0119968533259538 2307 96-97 0.7747088206308987 2307 98-99 0.9709816514407166 2307 100-101 0.9010340743560192 1101 1 0.8543611549829926 1101 2 0.5563551505518802 1101 3 0.4561516534397754 1101 4 0.3334133458665107 1101 5 0.576309608464264 1101 6 0.4531432720233113 1101 7 0.7207762064278356 1101 8 0.5878204775509346 1101 9 0.9326959304363669 1101 10-11 1.0507067090958842 1101 12-13 1.3664449691236555 1101 14-15 1.3162436676522518 1101 16-17 1.3836071470638558 1101 18-19 1.0951965475609668 1101 20-21 1.2848298036803314 1101 22-23 1.1132910353941767 1101 24-25 1.2546374740497512 1101 26-27 1.325273360391364 1101 28-29 1.6426034028642675 1101 30-31 1.5599226151924057 1101 32-33 0.9883077787427794 1101 34-35 1.1920564726229728 1101 36-37 1.138574213679746 1101 38-39 1.0118490361916983 1101 40-41 1.1288170987154729 1101 42-43 1.2129371792215977 1101 44-45 1.0661735126067597 1101 46-47 0.8932975811842034 1101 48-49 0.9804716633812909 1101 50-51 0.7934389027779716 1101 52-53 1.3431496746348586 1101 54-55 1.8471464157794948 1101 56-57 1.2790495991015973 1101 58-59 1.3711875396132065 1101 60-61 1.288207749297328 1101 62-63 0.7601299770660361 1101 64-65 0.729697437052149 1101 66-67 0.8461517038347459 1101 68-69 0.9261550232958733 1101 70-71 1.0745407545485754 1101 72-73 0.9604497801714871 1101 74-75 0.936664416524831 1101 76-77 1.1275501071100926 1101 78-79 1.0585599325785502 1101 80-81 1.7521253570854682 1101 82-83 1.6845443564410623 1101 84-85 1.482142741149186 1101 86-87 0.7616196167695222 1101 88-89 0.9642838993365004 1101 90-91 0.8026657430120423 1101 92-93 1.0634648558620583 1101 94-95 0.7347617815715637 1101 96-97 0.5216131557681472 1101 98-99 0.7434731201939471 1101 100-101 0.7619750392773739 2201 1 1.5048114018561236 2201 2 0.6773207238963614 2201 3 0.3697669607036076 2201 4 0.23388167040580754 2201 5 0.3188237598873158 2201 6 0.609299282282386 2201 7 0.6126305230355626 2201 8 0.5946860362073139 2201 9 0.625624738327268 2201 10-11 0.921872549085947 2201 12-13 1.110127954650224 2201 14-15 0.9676638943009905 2201 16-17 1.1285064205676463 2201 18-19 0.9310795850608287 2201 20-21 1.2276495806471246 2201 22-23 0.8749966067049186 2201 24-25 0.8494890112162992 2201 26-27 1.071116344562114 2201 28-29 1.4967494026157055 2201 30-31 1.380490762344607 2201 32-33 1.0034176005647737 2201 34-35 1.1134053489830364 2201 36-37 1.2397701642825254 2201 38-39 0.8648589676095497 2201 40-41 1.0440319969721088 2201 42-43 1.2579929375231984 2201 44-45 1.1204195044492096 2201 46-47 0.8722450090712606 2201 48-49 1.318750839039712 2201 50-51 1.1869405467157392 2201 52-53 1.3141886884981702 2201 54-55 1.4857865798343148 2201 56-57 1.1592401490016044 2201 58-59 1.2677827241748822 2201 60-61 1.2232097830347755 2201 62-63 1.0179722946228438 2201 64-65 1.014957359996096 2201 66-67 1.062724404299118 2201 68-69 0.9734281203390438 2201 70-71 0.8438440299957186 2201 72-73 0.9518835085771471 2201 74-75 1.2687647023779292 2201 76-77 1.2548626360626045 2201 78-79 0.9812219819079822 2201 80-81 1.3212258237152277 2201 82-83 1.4308158377928173 2201 84-85 1.3870770966238943 2201 86-87 1.2142578261766879 2201 88-89 1.072533529309272 2201 90-91 1.2097149029654908 2201 92-93 1.22406966678097 2201 94-95 1.196910538167085 2201 96-97 0.8491671910421914 2201 98-99 1.0544005044686386 2201 100-101 1.0231891240390283 2304 1 -1.7639367784268813 2304 2 -0.4144112099532862 2304 3 -0.030602077903505887 2304 4 -0.15137425459273146 2304 5 0.5479370481887216 2304 6 0.848923983848799 2304 7 0.2716803396080891 2304 8 0.6822952192271714 2304 9 0.92711326683132 2304 10-11 0.8832483279248251 2304 12-13 1.1852236717643052 2304 14-15 1.1923487193170033 2304 16-17 1.3364200058468683 2304 18-19 0.8397789241396083 2304 20-21 1.269595877159091 2304 22-23 1.1378820227650195 2304 24-25 1.353532422384994 2304 26-27 1.289194141103195 2304 28-29 1.5503746428183405 2304 30-31 1.5838677931487766 2304 32-33 1.0968109360332434 2304 34-35 1.0983854048847377 2304 36-37 1.4648371298680374 2304 38-39 1.1783387032410673 2304 40-41 1.3166543547430294 2304 42-43 1.1377649633777338 2304 44-45 1.3841485413093935 2304 46-47 1.0826632528259985 2304 48-49 0.8316628229679743 2304 50-51 1.22525577993067 2304 52-53 1.6385070225108649 2304 54-55 1.9585126614740922 2304 56-57 1.2943337552439615 2304 58-59 1.0517630275580991 2304 60-61 0.9823021809850445 2304 62-63 0.7309250402118508 2304 64-65 1.1758010535848662 2304 66-67 1.1256436670953676 2304 68-69 1.0624064584279047 2304 70-71 0.9945249680962256 2304 72-73 0.5596748088741279 2304 74-75 0.7766615462607653 2304 76-77 0.9365053309906877 2304 78-79 0.9962608510630488 2304 80-81 1.540005667073988 2304 82-83 1.2069324161425534 2304 84-85 0.9673393542375912 2304 86-87 1.2095458509830728 2304 88-89 0.8615930267762231 2304 90-91 0.7574016787181286 2304 92-93 0.95788219225701 2304 94-95 0.950275558839067 2304 96-97 0.8407004117957015 2304 98-99 1.1404378159459583 2304 100-101 1.2413091380144579 1102 1 1.4134792699917043 1102 2 0.13448733228281995 1102 3 0.30021433495605265 1102 4 0.27027093608798936 1102 5 0.25593685295248036 1102 6 0.4028970412482522 1102 7 -4.788483333436293 1102 8 -1.2964093883479961 1102 9 0.13675305597618603 1102 10-11 0.7403492325193852 1102 12-13 0.8519969048763656 1102 14-15 0.9618544462465834 1102 16-17 0.8573753618276001 1102 18-19 1.0792483696687043 1102 20-21 1.1811009858405939 1102 22-23 0.6039475245221482 1102 24-25 0.9632145127142948 1102 26-27 1.152945279981239 1102 28-29 1.0280404309219477 1102 30-31 1.1996876226424718 1102 32-33 1.1084071549581438 1102 34-35 1.3008860556721942 1102 36-37 1.311827237898754 1102 38-39 0.9219268198621791 1102 40-41 1.3178509901718982 1102 42-43 1.49074231597438 1102 44-45 1.1569480662345555 1102 46-47 1.0765930328598365 1102 48-49 1.0207670140391691 1102 50-51 0.7028865882086919 1102 52-53 0.7676452172264732 1102 54-55 1.2867535829891317 1102 56-57 0.9874435430872524 1102 58-59 0.910463242317963 1102 60-61 0.8737565282452309 1102 62-63 0.5372620830336636 1102 64-65 0.2858191129366823 1102 66-67 0.3706472464263584 1102 68-69 0.1772346505403597 1102 70-71 0.3277663777018489 1102 72-73 0.2543123202629083 1102 74-75 0.3327285122896626 1102 76-77 0.44758824131217523 1102 78-79 0.553870183355123 1102 80-81 0.8328543264518338 1102 82-83 1.0910271707956838 1102 84-85 0.49735285807723884 1102 86-87 0.26746349383091683 1102 88-89 0.3406596348720221 1102 90-91 0.05384512528847907 1102 92-93 0.08275040779781051 1102 94-95 -0.10132049738896853 1102 96-97 -0.14645955176019854 1102 98-99 0.1313812950556823 1102 100-101 0.14440363235260278 2202 1 1.4832189645709377 2202 2 0.877162968753634 2202 3 0.3243690658530518 2202 4 -0.3460630914641527 2202 5 0.20613686254016272 2202 6 0.5981793716928578 2202 7 0.6589220459605016 2202 8 -3.819433128500652 2202 9 -0.5060340816229569 2202 10-11 0.6379657183518148 2202 12-13 0.021375197311158445 2202 14-15 -0.019283575460626423 2202 16-17 0.056696057182726634 2202 18-19 0.7789916329942344 2202 20-21 1.0009023788945015 2202 22-23 -3.2907265211045456 2202 24-25 -3.136251862060881 2202 26-27 -1.5845365877691933 2202 28-29 0.4583650980608951 2202 30-31 1.1244607586827158 2202 32-33 0.8116108593523208 2202 34-35 -2.1148733327050557 2202 36-37 -0.3555660503725022 2202 38-39 0.007276672407265039 2202 40-41 1.0874555695303414 2202 42-43 1.270606597085063 2202 44-45 1.4374120210091306 2202 46-47 0.8457666106434125 2202 48-49 0.9230876756598754 2202 50-51 -4.219419940466452 2202 52-53 -0.523622184850506 2202 54-55 1.372942801114096 2202 56-57 1.2485461614107223 2202 58-59 1.456219097458817 2202 60-61 1.309568808638609 2202 62-63 1.105794884428498 2202 64-65 0.8938922029915979 2202 66-67 1.090660012240253 2202 68-69 1.3390772954607577 2202 70-71 0.8595468625186946 2202 72-73 0.9668666726242279 2202 74-75 0.7443980463818427 2202 76-77 1.2619512911166062 2202 78-79 1.0711932507724704 2202 80-81 1.7238779529727672 2202 82-83 1.5912451128896699 2202 84-85 1.3006314883888521 2202 86-87 1.4901430541471683 2202 88-89 1.4209102638413604 2202 90-91 1.0839393330889386 2202 92-93 1.1277778658384108 2202 94-95 1.0285112921508777 2202 96-97 1.054333473382595 2202 98-99 1.3800763057352867 2202 100-101 1.2832758019905626 2305 1 1.2050955781054782 2305 2 0.5633055177634425 2305 3 0.03249316019173065 2305 4 -0.3147594804779601 2305 5 0.19812019837186767 2305 6 0.3458256932505108 2305 7 0.5008164812442288 2305 8 -0.2692157697838198 2305 9 0.2457793229973717 2305 10-11 0.10208227175876772 2305 12-13 0.7331708634614955 2305 14-15 0.5154485361668222 2305 16-17 0.5746297128065692 2305 18-19 0.6251879595486471 2305 20-21 0.919664558477777 2305 22-23 0.6447883109213066 2305 24-25 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1208 36-37 -2.587140794168711 1208 38-39 -0.24417432294842456 1208 40-41 -0.5379313781515265 1208 42-43 0.4255306290845766 1208 44-45 0.20969786943225444 1208 46-47 -0.15612908228987266 1208 48-49 -1.0756095615102943 1208 50-51 0.3200744416610988 1208 52-53 -0.6221907356133656 1208 54-55 -0.8758661949635815 1208 56-57 -5.245013906897817 1208 58-59 -1.4808770039789891 1208 60-61 -1.4619791504286397 1208 62-63 0.8510350772336537 1208 64-65 0.021966040558677236 1208 66-67 1.035624127811122 1208 68-69 0.8831215276723832 1208 70-71 -4.0417966759460064 1208 72-73 -0.7230757738764559 1208 74-75 -5.28457697012481 1208 76-77 -1.2906782697223242 1208 78-79 -3.8606927345670066 1208 80-81 0.7611278047402443 1208 82-83 0.4301944482281179 1208 84-85 -2.4801573892150337 1208 86-87 -0.6736066582723801 1208 88-89 -5.282760291474151 1208 90-91 -0.028547176348375558 1208 92-93 -1.8741159566529078 1208 94-95 -5.01757546268842 1208 96-97 -3.4834597417762154 1208 98-99 -6.390861889250807 1208 100-101 -3.9009197115673366 1209 1 1.5514674601916454 1209 2 0.9971207105072075 1209 3 0.1528162894434324 1209 4 0.3347586601116106 1209 5 -0.02254306693425434 1209 6 1.0985184370146825 1209 7 0.9214514018791533 1209 8 0.1287434138896515 1209 9 -0.0124406316511525 1209 10-11 -2.0655055967219624 1209 12-13 0.2070937659558183 1209 14-15 -1.5961586066903237 1209 16-17 -0.041654300798875 1209 18-19 -16.627008752469493 1209 20-21 -13.885182380297735 1209 22-23 -2.8112774487158845 1209 24-25 -2.0487765781382876 1209 26-27 -1.3745159583211901 1209 28-29 -2.6706974398608807 1209 30-31 -0.6950601241719951 1209 32-33 -0.5323262957825534 1209 34-35 -3.6421627374491123 1209 36-37 -4.148729065841017 1209 38-39 -1.3760634422325069 1209 40-41 -10.773916681363723 1209 42-43 -6.125853560955072 1209 44-45 -3.242228355603217 1209 46-47 -2.862829944452919 1209 48-49 -3.618317553798118 1209 50-51 -1.832914026453416 1209 52-53 -0.8774564782740661 1209 54-55 -2.768004868614863 1209 56-57 -0.7598198292667675 1209 58-59 -2.5730047641739766 1209 60-61 -2.923836620689471 1209 62-63 -1.649510595580928 1209 64-65 -0.42077469209801777 1209 66-67 -0.017651727985739996 1209 68-69 -1.9157070946856471 1209 70-71 -0.6838534986751021 1209 72-73 0.15323838815199053 1209 74-75 -0.19457733390668608 1209 76-77 -16.66315453295489 1209 78-79 -15.692952911364998 1209 80-81 -21.291165839990377 1209 82-83 -10.50905397841527 1209 84-85 -3.961640891706942 1209 86-87 -2.5187083353068296 1209 88-89 -2.868394545175633 1209 90-91 -6.708309990564974 1209 92-93 -4.2823519783343045 1209 94-95 -0.8192233945884553 1209 96-97 0.6930290355528967 1209 98-99 1.1849138756005893 1209 100-101 0.1633787350840521 1212 1 -28.63220600919611 1212 2 -16.22370608174816 1212 3 -5.96788840268983 1212 4 -1.8009469041653858 1212 5 -0.5508877381360691 1212 6 -2.4645375546127397 1212 7 -0.7861188056910535 1212 8 -1.8357603363283843 1212 9 -0.8093183571003024 1212 10-11 0.5468701227966122 1212 12-13 -0.01621863592801276 1212 14-15 -0.2957923063240244 1212 16-17 -0.0631526437257861 1212 18-19 -1.5648685117578225 1212 20-21 -1.1179575416443228 1212 22-23 -0.8602483191153212 1212 24-25 0.02500678135935175 1212 26-27 -0.7915750896660398 1212 28-29 -0.36976424607054525 1212 30-31 -0.09855742052643279 1212 32-33 0.3011378591101703 1212 34-35 -1.1756530566537151 1212 36-37 -1.6670004769695694 1212 38-39 -1.5544604420580796 1212 40-41 -0.8452580384193631 1212 42-43 -0.4363803357675593 1212 44-45 0.24168059259144314 1212 46-47 -2.708896576233837 1212 48-49 -1.4280593992542485 1212 50-51 -0.8421587499838559 1212 52-53 -1.9441318663780223 1212 54-55 -0.294692466731032 1212 56-57 -1.1899611165509114 1212 58-59 -0.39177329722822307 1212 60-61 -0.5928580754252124 1212 62-63 -4.363839917053106 1212 64-65 -4.1235045019706895 1212 66-67 -2.1295913756396736 1212 68-69 0.30193637295781883 1212 70-71 -0.6981566558354011 1212 72-73 -1.7599833107839942 1212 74-75 -0.9200264879272666 1212 76-77 0.6745395190248757 1212 78-79 -1.122222054919856 1212 80-81 0.696362504680824 1212 82-83 0.5616332708434086 1212 84-85 -2.8817525261042896 1212 86-87 -1.3757639780767583 1212 88-89 -0.23813987920668112 1212 90-91 0.8165897129061612 1212 92-93 -2.0494896026147877 1212 94-95 -1.8773953813318727 1212 96-97 0.10437562547091517 1212 98-99 -0.04903867978053711 1212 100-101 -0.21787889617358047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 0.0 11 5.0 12 25.0 13 80.0 14 285.0 15 639.0 16 1254.0 17 2053.0 18 2987.0 19 4090.0 20 5622.0 21 7395.0 22 9213.0 23 11558.0 24 14174.0 25 17082.0 26 20588.0 27 23596.0 28 27936.0 29 32593.0 30 37282.0 31 42988.0 32 48463.0 33 54060.0 34 58289.0 35 62578.0 36 63784.0 37 55154.0 38 19041.0 39 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.30639383211036 20.655055240264016 14.746587007257006 21.291963920368627 2 25.06827650983005 25.645957597116407 27.04295680236339 22.242809090690148 3 21.435073765398897 23.82343827516284 29.907217272775437 24.834270686662823 4 24.748215068402978 24.492456486522503 27.2163736868892 23.54295475818532 5 22.779335125085492 26.861190583989185 26.34437604960392 24.0150982413214 6 22.09378120715288 27.166559097316544 26.374720239414053 24.36493945611652 7 24.096497734621597 26.037241472319355 27.3515141590164 22.51474663404265 8 22.758142357599052 25.805887093925705 27.607272330273226 23.828698218202018 9 23.143465402807077 25.60439525153567 27.245871424121866 24.006267921535386 10-11 23.961795220067625 25.964270920633087 26.082918308303388 23.9910155509959 12-13 23.832028639195837 25.902032523921626 26.107448459018556 24.15849037786398 14-15 23.54733211956574 26.258079742604206 26.221233226406188 23.973354911423865 16-17 23.92923829294647 26.13771190862662 25.98639160890807 23.94665818951884 18-19 24.06628098934431 26.229165628531337 25.665759537315807 24.038793844808545 20-21 23.70483291429452 26.288263381145498 26.02158772360778 23.985315980952198 22-23 23.88713888005857 26.047275926621648 25.968284702354005 24.097300490965782 24-25 23.84411114001034 25.86023369842692 26.166404968098462 24.12925019346428 26-27 23.87493698362698 26.063892982946246 26.08653071185222 23.97463932157456 28-29 24.151004890391647 26.023434063199403 26.01388126270362 23.81167978370533 30-31 24.0855802483407 25.829889508616787 25.901575650152363 24.182954592890145 32-33 23.835120268955485 26.115349664608402 26.007619763218987 24.041910303217126 34-35 24.26017975320059 25.92919046839227 25.81230914467917 23.998320633727968 36-37 24.116887745763854 25.988193059689753 25.835589078660487 24.059330115885906 38-39 23.84692078721498 25.97141545209632 26.276864241058096 23.904799519630604 40-41 24.287663865006543 25.87297000056194 25.78771604492289 24.051650089508627 42-43 23.919730787632414 25.96708056783773 25.98209211147396 24.131096533055903 44-45 23.770337831979887 25.909362386690944 26.199639080747655 24.120660700581517 46-47 24.246543554941397 25.79621286514585 25.893769767316748 24.063473812596 48-49 24.140787746697654 25.74903367972353 26.048542793036876 24.06163578054194 50-51 23.946401521385045 25.966700034598826 26.110072432763083 23.976826011253046 52-53 24.161797925988722 25.927308457399924 25.89479666886623 24.016096947745126 54-55 23.93014257273136 25.72225611714084 26.15711900327532 24.19048230685248 56-57 23.816140895068045 25.95647563243913 26.159254326708105 24.068129145784717 58-59 24.15369495812973 25.798693226425723 26.043863941904743 24.003747873539805 60-61 24.0849872320066 25.735393873129254 25.951980142779963 24.227638752084182 62-63 23.913436135548647 25.93014490729257 26.09350779044275 24.062911166716034 64-65 24.26618628801526 25.732288445544775 25.896856666698774 24.104668599741185 66-67 24.167147001825544 25.75891617121232 25.989557326328733 24.0843795006334 68-69 23.92979740730156 25.93557430165086 25.994258428517757 24.140369862529823 70-71 24.318897938049155 25.77675378211638 25.76302617477412 24.14132210506035 72-73 24.09171325113694 25.802723836338537 25.982931653535214 24.122631258989312 74-75 23.966092574820003 25.82899317755621 26.229104007131642 23.975810240492148 76-77 24.19592036272734 25.875520327027097 25.917090205027144 24.011469105218428 78-79 24.137637612727243 25.794288539992582 25.89383048648718 24.174243360792996 80-81 23.972924228346937 25.825020173410962 26.044838017958355 24.157217580283746 82-83 24.288185733610028 25.813166566986844 25.821194471984622 24.07745322741851 84-85 24.08290925789005 25.652771121285173 26.00640147200139 24.257918148823386 86-87 23.898551096953195 25.907491137029236 26.027430252273543 24.166527513744025 88-89 24.206884094330157 25.787295534370298 25.817400903161065 24.188419468138484 90-91 24.16092240123008 25.689934119933035 25.748949170778317 24.400194308058566 92-93 23.928316528672827 25.879525075325933 25.949050527983996 24.243107868017248 94-95 24.251518640377288 25.76590377367883 25.682244071160525 24.30033351478336 96-97 24.188135742900954 25.716006069412888 25.650575229409355 24.445282958276803 98-99 24.005661633122642 25.810367404022394 25.85637018024578 24.327600782609185 100-101 24.259590658345918 25.67325084841288 25.51637374644839 24.55078474679281 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.5 5 1.5 6 4.0 7 5.5 8 8.5 9 19.5 10 31.0 11 39.0 12 57.0 13 91.5 14 146.5 15 235.0 16 332.0 17 482.0 18 752.5 19 1081.5 20 1443.0 21 1911.0 22 2507.0 23 3254.0 24 4138.0 25 5140.0 26 6302.5 27 7441.5 28 8531.0 29 9699.5 30 10872.0 31 12023.5 32 13029.0 33 13878.0 34 14724.0 35 15441.0 36 16041.0 37 16560.5 38 16723.5 39 16755.0 40 16671.0 41 16241.5 42 15593.0 43 15060.5 44 14439.0 45 13742.0 46 13435.5 47 13066.5 48 12629.0 49 12155.5 50 11645.0 51 11234.5 52 10835.5 53 10538.5 54 10320.0 55 10188.5 56 10259.5 57 10429.5 58 10446.0 59 10485.5 60 10768.0 61 10964.5 62 11033.5 63 11288.5 64 11512.5 65 11581.5 66 11690.0 67 11555.5 68 11221.0 69 10911.0 70 10326.5 71 9594.0 72 8605.5 73 7464.5 74 6443.0 75 5222.0 76 4045.5 77 3099.5 78 2209.0 79 1488.0 80 969.0 81 598.5 82 386.0 83 251.5 84 158.0 85 111.0 86 75.5 87 49.0 88 33.5 89 23.0 90 17.0 91 12.0 92 10.5 93 6.5 94 4.5 95 3.0 96 1.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7313110295510665 2 0.0030504741078968747 3 1.605512688366776E-4 4 1.605512688366776E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.19081518301239134 14-15 0.0 16-17 4.816538065100328E-4 18-19 0.11961069528332482 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0014449614195300986 42-43 0.0 44-45 0.0 46-47 0.022557453271553205 48-49 2.408269032550164E-4 50-51 8.02756344183388E-5 52-53 4.816538065100328E-4 54-55 0.0022477177637134863 56-57 0.001204134516275082 58-59 0.10186978007687195 60-61 0.001204134516275082 62-63 0.001204134516275082 64-65 0.0019266152260401313 66-67 0.004013781720916941 68-69 0.004495435527426973 70-71 0.003451852279988569 72-73 0.03845202888638429 74-75 0.04439242583334136 76-77 0.16207650589062605 78-79 1.605512688366776E-4 80-81 0.12009234908983486 82-83 0.004254608624171956 84-85 0.004575711161845312 86-87 0.006100948215793749 88-89 0.006662877656722121 90-91 0.02111249185202311 92-93 0.009713351764618995 94-95 0.014931268001811018 96-97 0.00947252486136398 98-99 0.010516108108802383 100-101 0.011800518259495806 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 622854.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.4058480943475 #Duplication Level Percentage of deduplicated Percentage of total 1 99.49091432364725 98.8997871602422 2 0.4492299154111332 0.8931216146159138 3 0.046698589353321156 0.1392633863842967 4 0.012817941989445211 0.05096713577139795 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 3.392295988500853E-4 0.01686070298619901 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.6055126883667763E-4 0.0 0.0 2 1.6055126883667763E-4 0.0 0.0 3 1.6055126883667763E-4 0.0 0.0 4 1.6055126883667763E-4 0.0 0.0 5 1.6055126883667763E-4 0.0 0.0 6 1.6055126883667763E-4 0.0 0.0 7 1.6055126883667763E-4 0.0 0.0 8 3.2110253767335525E-4 0.0 0.0 9 3.2110253767335525E-4 0.0 0.0 10-11 6.422050753467105E-4 8.027563441833881E-5 0.0 12-13 6.422050753467105E-4 1.6055126883667763E-4 0.0 14-15 6.422050753467105E-4 1.6055126883667763E-4 0.0 16-17 6.422050753467105E-4 1.6055126883667763E-4 0.0 18-19 6.422050753467105E-4 1.6055126883667763E-4 0.0 20-21 6.422050753467105E-4 1.6055126883667763E-4 0.0 22-23 6.422050753467105E-4 2.4082690325501644E-4 0.0 24-25 7.224807097650493E-4 3.2110253767335525E-4 0.0 26-27 8.027563441833881E-4 3.2110253767335525E-4 0.0 28-29 0.0011238588818567434 3.2110253767335525E-4 0.0 30-31 0.001284410150693421 3.2110253767335525E-4 0.0 32-33 0.0016857883227851152 3.2110253767335525E-4 0.0 34-35 0.002167442129295148 3.2110253767335525E-4 0.0 36-37 0.0027293715702235194 3.2110253767335525E-4 0.0 38-39 0.0039335060864986015 3.2110253767335525E-4 0.0 40-41 0.005539018774865378 3.2110253767335525E-4 0.0 42-43 0.007867012172997203 3.2110253767335525E-4 0.0 44-45 0.011078037549730756 3.2110253767335525E-4 0.0 46-47 0.016536780690177794 3.2110253767335525E-4 0.0 48-49 0.022718004540389882 3.2110253767335525E-4 0.0 50-51 0.02841757458409194 3.2110253767335525E-4 0.0 52-53 0.036846516198017515 3.2110253767335525E-4 0.0 54-55 0.048085105016584945 3.2110253767335525E-4 0.0 56-57 0.06269527048072261 3.2110253767335525E-4 0.0 58-59 0.08043618568717548 3.2110253767335525E-4 0.0 60-61 0.10475970291593215 3.2110253767335525E-4 0.0 62-63 0.13229424552142235 3.2110253767335525E-4 1.6055126883667763E-4 64-65 0.16159485208411603 3.2110253767335525E-4 1.6055126883667763E-4 66-67 0.1971569581314401 3.2110253767335525E-4 1.6055126883667763E-4 68-69 0.2420310377712915 3.2110253767335525E-4 1.6055126883667763E-4 70-71 0.29340744379902833 3.2110253767335525E-4 1.6055126883667763E-4 72-73 0.34847652901000875 3.2110253767335525E-4 1.6055126883667763E-4 74-75 0.41542640811490333 3.2110253767335525E-4 2.4082690325501644E-4 76-77 0.494257081113712 3.2110253767335525E-4 3.2110253767335525E-4 78-79 0.5828011058771397 3.2110253767335525E-4 3.2110253767335525E-4 80-81 0.6869186037177253 3.2110253767335525E-4 3.2110253767335525E-4 82-83 0.8006691776885113 3.2110253767335525E-4 3.2110253767335525E-4 84-85 0.9243739303271714 3.2110253767335525E-4 3.2110253767335525E-4 86-87 1.0599594768597456 3.2110253767335525E-4 3.2110253767335525E-4 88-89 1.2202699187931683 3.2110253767335525E-4 3.2110253767335525E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGGAT 295 0.0049315924 11.34526 1 CTGAACT 755 1.8189894E-10 7.544056 94-95 GTCTGAA 785 3.245077E-9 6.9537044 92-93 ACACGTC 805 5.467882E-9 6.781486 88-89 ACGTCTG 740 5.4153134E-8 6.7359357 90-91 CCAGTCA 505 1.4204161E-4 6.580056 88-89 TCTGAAC 740 3.8621874E-7 6.414147 94-95 CGTCTGA 690 5.4768043E-6 6.1912947 92-93 CACACGT 775 8.857951E-7 6.1247215 92-93 CAGTCAC 475 0.003413231 5.9962616 88-89 ACCATTT 615 2.8479262E-4 5.788377 94-95 CACGTCT 830 3.0038573E-6 5.7186365 94-95 GCACACG 960 6.896291E-6 5.1922836 90-91 >>END_MODULE