FastQCFastQC Report
Thu 11 Sep 2014
LONLib34_CATGGC_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib34_CATGGC_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3182387
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGG6156.39802E-59.5581294
TGATACG5954.164469E-49.1168133
GTTACGC5000.00701406929.005281
GTGCGTT7300.00219389917.711
GTGCGCT8500.0096436766.62152961
GATAACT15501.0402492E-45.80985831
GTCCTTC13600.00460958435.3799931
GGTCAAA16150.00112459555.22752331
GGATCAC16800.00189894385.0252681
GTAATGT16850.00197502555.01035641
Produced by FastQC (version 0.11.2)