##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib34_CATGGC_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3182387 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.230638825510535 33.0 33.0 33.0 27.0 33.0 2 31.62206387846607 33.0 33.0 33.0 27.0 33.0 3 31.815678922770864 33.0 33.0 33.0 27.0 33.0 4 35.65368731081418 37.0 37.0 37.0 33.0 37.0 5 35.174901732567406 37.0 37.0 37.0 33.0 37.0 6 34.98684100959437 37.0 37.0 37.0 33.0 37.0 7 34.798424578783155 37.0 33.0 37.0 27.0 37.0 8 34.83553791540752 37.0 33.0 37.0 27.0 37.0 9 34.73959923793052 37.0 33.0 37.0 27.0 37.0 10-11 34.602142982610225 37.0 33.0 37.0 27.0 37.0 12-13 34.60679955014899 37.0 33.0 37.0 27.0 37.0 14-15 35.568572426923566 37.0 33.0 40.0 27.0 40.0 16-17 35.4817278665354 37.0 33.0 40.0 27.0 40.0 18-19 35.698371065492665 37.0 33.0 40.0 27.0 40.0 20-21 35.380601102254374 37.0 33.0 40.0 27.0 40.0 22-23 35.40992233188484 37.0 33.0 40.0 27.0 40.0 24-25 35.25013739686594 37.0 33.0 40.0 27.0 40.0 26-27 34.882948868255184 37.0 33.0 40.0 27.0 40.0 28-29 34.52946404695595 37.0 33.0 40.0 27.0 40.0 30-31 34.41663851693713 37.0 33.0 40.0 27.0 40.0 32-33 34.407943942707156 37.0 33.0 40.0 27.0 40.0 34-35 34.26843623984135 37.0 33.0 40.0 24.5 40.0 36-37 33.96057896164106 37.0 33.0 38.5 22.0 40.0 38-39 33.862715471122776 37.0 33.0 37.0 22.0 40.0 40-41 33.58065628096143 37.0 33.0 37.0 22.0 40.0 42-43 33.218298403054064 37.0 33.0 37.0 22.0 40.0 44-45 33.08862875571073 37.0 33.0 37.0 22.0 40.0 46-47 32.96302570994665 37.0 33.0 37.0 22.0 40.0 48-49 32.97795962590345 37.0 33.0 37.0 22.0 40.0 50-51 33.27040787308395 37.0 33.0 37.0 22.0 40.0 52-53 33.13486354739383 37.0 33.0 37.0 22.0 40.0 54-55 32.79563422047664 37.0 33.0 37.0 22.0 40.0 56-57 32.845812592874466 37.0 33.0 37.0 22.0 40.0 58-59 32.571889748167024 37.0 33.0 37.0 22.0 40.0 60-61 32.49510163283095 37.0 33.0 37.0 22.0 40.0 62-63 32.47825861530983 37.0 33.0 37.0 22.0 40.0 64-65 32.150450903677026 35.0 27.0 37.0 22.0 40.0 66-67 31.817352195066157 33.0 27.0 37.0 18.5 40.0 68-69 31.648276278152217 33.0 27.0 37.0 22.0 40.0 70-71 31.496637429702922 33.0 27.0 37.0 18.5 40.0 72-73 31.285832301351157 33.0 27.0 37.0 15.0 38.5 74-75 30.94964314522401 33.0 27.0 37.0 15.0 37.0 76-77 29.8821876471969 33.0 27.0 37.0 15.0 37.0 78-79 30.819152258980445 33.0 27.0 37.0 15.0 37.0 80-81 30.542910400275012 33.0 27.0 37.0 15.0 37.0 82-83 30.513660814979445 33.0 27.0 37.0 15.0 37.0 84-85 30.38689072070744 33.0 27.0 37.0 15.0 37.0 86-87 30.187525275838546 33.0 27.0 37.0 15.0 37.0 88-89 30.049278418998068 33.0 27.0 37.0 15.0 37.0 90-91 29.95221684226337 33.0 27.0 37.0 15.0 37.0 92-93 29.73496639472195 33.0 27.0 37.0 15.0 37.0 94-95 29.641467395385916 33.0 27.0 37.0 15.0 37.0 96-97 29.500811654899294 33.0 27.0 37.0 15.0 37.0 98-99 29.2376627983963 33.0 27.0 33.0 15.0 37.0 100-101 28.444434790614718 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.164502166439572 2306 2 0.5756967332594094 2306 3 0.4673059548885625 2306 4 0.31005721756058335 2306 5 0.628378275813354 2306 6 0.6604729334694213 2306 7 0.7635200375094229 2306 8 0.7688139368440048 2306 9 1.0278949023343529 2306 10-11 1.1441118803983272 2306 12-13 1.3389879033126633 2306 14-15 1.4186240034765092 2306 16-17 1.37898079762072 2306 18-19 1.2573439342243375 2306 20-21 1.526337752822009 2306 22-23 1.241161509309137 2306 24-25 1.1707309427754993 2306 26-27 1.2862651248285815 2306 28-29 1.548769685255742 2306 30-31 1.4682103030521745 2306 32-33 1.214347301385473 2306 34-35 1.121255069908898 2306 36-37 1.2673255946024753 2306 38-39 1.076902593132445 2306 40-41 1.3184693380534895 2306 42-43 1.3176970008067954 2306 44-45 1.0903834069672271 2306 46-47 0.912441319031501 2306 48-49 1.0171632144198952 2306 50-51 0.931851780709529 2306 52-53 1.0988769959491194 2306 54-55 1.4735915928279297 2306 56-57 1.0432437220205415 2306 58-59 1.036346825615631 2306 60-61 0.8280934123224597 2306 62-63 0.650258904104625 2306 64-65 0.7270509104560894 2306 66-67 0.8404187680149562 2306 68-69 0.6672941773889391 2306 70-71 0.7857619223398942 2306 72-73 0.48030746043600914 2306 74-75 0.7935742030476582 2306 76-77 0.765714670894198 2306 78-79 0.8612903682386595 2306 80-81 1.2884414339687247 2306 82-83 1.1204923835358436 2306 84-85 0.9102371579144481 2306 86-87 0.7784925131818596 2306 88-89 0.5800350083741286 2306 90-91 0.33445638879675954 2306 92-93 0.1289029604849361 2306 94-95 0.15898698090451546 2306 96-97 0.26101380249944484 2306 98-99 0.3412720669827287 2306 100-101 0.3057133093393034 2307 1 0.6900452949139861 2307 2 0.5164845896325154 2307 3 0.3836726131630819 2307 4 0.31556808357718324 2307 5 0.5232614898651349 2307 6 0.567651514179083 2307 7 0.7448871247926476 2307 8 0.752752987230771 2307 9 0.9591021909238862 2307 10-11 1.1107836174528174 2307 12-13 1.2935791645587855 2307 14-15 1.272122062366165 2307 16-17 1.2796463638440017 2307 18-19 1.2416426645946288 2307 20-21 1.4776151907598418 2307 22-23 1.1951521688065583 2307 24-25 1.2602784730234333 2307 26-27 1.3638869859174534 2307 28-29 1.5059855146179544 2307 30-31 1.5152051100934187 2307 32-33 1.1978143690703007 2307 34-35 1.2221106722622963 2307 36-37 1.3633929291021047 2307 38-39 1.0474785914875184 2307 40-41 1.1906212848118614 2307 42-43 1.2647553725864 2307 44-45 1.0153636659856957 2307 46-47 1.009721117593159 2307 48-49 1.0214981662034717 2307 50-51 1.0911007227753018 2307 52-53 1.2537500909524937 2307 54-55 1.475427252888494 2307 56-57 1.1812382752081234 2307 58-59 1.1101873619394027 2307 60-61 0.966493878572507 2307 62-63 0.8051239871849347 2307 64-65 0.9922768703988574 2307 66-67 1.0830759240797647 2307 68-69 0.8869815469275082 2307 70-71 0.8036633579699632 2307 72-73 0.7221119264344402 2307 74-75 0.8462869326104077 2307 76-77 0.9577130536347731 2307 78-79 0.9522482685390408 2307 80-81 1.45169309937803 2307 82-83 1.2511709332528334 2307 84-85 1.0851506004192686 2307 86-87 1.0264850146068234 2307 88-89 0.877875982084138 2307 90-91 0.6064834372997758 2307 92-93 0.638625309014202 2307 94-95 0.6231450632708864 2307 96-97 0.524212610108016 2307 98-99 0.7045486832127175 2307 100-101 0.7781864934745428 1101 1 0.9966190075407226 1101 2 0.5742140092348151 1101 3 0.36637056067278806 1101 4 0.31550991294448494 1101 5 0.6260929475566286 1101 6 0.5146934163175914 1101 7 0.741328870947207 1101 8 0.6862785181648832 1101 9 0.8731969502410664 1101 10-11 0.9765935235040288 1101 12-13 1.1873411320886333 1101 14-15 1.2000237457603617 1101 16-17 1.2300907332109645 1101 18-19 1.0760578673093093 1101 20-21 1.2703152396622173 1101 22-23 0.9454130733174253 1101 24-25 1.059999161754284 1101 26-27 1.107288646221427 1101 28-29 1.269760066265981 1101 30-31 1.3041318432592135 1101 32-33 0.974767475411042 1101 34-35 0.9803259281842358 1101 36-37 1.0812593439762992 1101 38-39 0.9589878249064725 1101 40-41 1.1047818507297578 1101 42-43 1.13681525410491 1101 44-45 0.9737898526542708 1101 46-47 0.8628547805157538 1101 48-49 0.767328890134209 1101 50-51 0.7935925021516752 1101 52-53 1.0549255066617746 1101 54-55 1.4578743073706733 1101 56-57 1.140106843961533 1101 58-59 0.9974873271936033 1101 60-61 0.8739024041264969 1101 62-63 0.7116992913533124 1101 64-65 0.5236561087552367 1101 66-67 0.7327631614794115 1101 68-69 0.7202192447663762 1101 70-71 0.8463896555123611 1101 72-73 0.6489176768245564 1101 74-75 0.7717202249976509 1101 76-77 0.7871876456268794 1101 78-79 0.8214048949623702 1101 80-81 1.3674993236485982 1101 82-83 1.0981093058217084 1101 84-85 0.9213898020933975 1101 86-87 0.7807605292984157 1101 88-89 0.6887797497329586 1101 90-91 0.6126419205717362 1101 92-93 0.7439777622394494 1101 94-95 0.553898265932073 1101 96-97 0.5403577490968985 1101 98-99 0.6781354945060514 1101 100-101 0.6432439563859944 2201 1 1.4413189578636647 2201 2 0.7797563486858827 2201 3 0.2965877101871648 2201 4 0.25645234573241993 2201 5 0.3902805816131547 2201 6 0.39651395736706974 2201 7 0.6146508790480567 2201 8 0.5495396616554018 2201 9 0.54593560727713 2201 10-11 0.7735326999361831 2201 12-13 1.0272379945908412 2201 14-15 0.9977176973592705 2201 16-17 0.9445054008899021 2201 18-19 0.8398432611245426 2201 20-21 1.2453854205682617 2201 22-23 0.905733764232842 2201 24-25 0.8283311626561769 2201 26-27 1.0488710200055706 2201 28-29 1.3655887087710852 2201 30-31 1.3875028423454125 2201 32-33 1.0001465662139708 2201 34-35 0.9741588169341497 2201 36-37 0.9551290453113666 2201 38-39 0.6270443781431396 2201 40-41 1.025722355738374 2201 42-43 0.9743948032619372 2201 44-45 0.8120092015065907 2201 46-47 0.810304511952701 2201 48-49 0.9596775061982186 2201 50-51 0.9707294872630783 2201 52-53 1.0491020175099095 2201 54-55 1.3633339923052716 2201 56-57 0.9833597120006488 2201 58-59 1.190806343627898 2201 60-61 0.8693887201541344 2201 62-63 0.7044293046860304 2201 64-65 0.6871293275354056 2201 66-67 0.8046434502097952 2201 68-69 0.7197670600845996 2201 70-71 0.7197909008212804 2201 72-73 0.6852064920785175 2201 74-75 0.7908345873535545 2201 76-77 0.7203705057992238 2201 78-79 0.783135573409389 2201 80-81 1.2463840772271872 2201 82-83 1.1229624311241473 2201 84-85 1.0474254834886096 2201 86-87 1.00911658700797 2201 88-89 0.9641622861044645 2201 90-91 0.7610403202975178 2201 92-93 0.856262712021973 2201 94-95 0.7342214563520599 2201 96-97 0.4887717288794775 2201 98-99 0.8412569174732702 2201 100-101 0.9085167054210359 2304 1 -1.877779389707662 2304 2 -0.21469834387056963 2304 3 -0.07808271411236944 2304 4 -0.005510943403436386 2304 5 0.4013730243222895 2304 6 0.5053620063110529 2304 7 0.4579072459338107 2304 8 0.678894184578894 2304 9 0.9057167039858598 2304 10-11 0.7840858105181994 2304 12-13 1.1317801379082582 2304 14-15 1.1781089997179208 2304 16-17 1.1517921630161396 2304 18-19 0.9021067589533658 2304 20-21 1.212153469507811 2304 22-23 0.920076448408345 2304 24-25 1.000263789246425 2304 26-27 1.073027571523518 2304 28-29 1.3471270553144024 2304 30-31 1.4947836723582952 2304 32-33 0.9701858627262538 2304 34-35 0.9451366031359143 2304 36-37 1.1294067675836743 2304 38-39 0.9231226087050572 2304 40-41 0.8748168970087633 2304 42-43 0.9099137280328833 2304 44-45 0.949172775627595 2304 46-47 0.8918291692166846 2304 48-49 0.7696602006006614 2304 50-51 0.7365455206300311 2304 52-53 1.0320061486868255 2304 54-55 1.3735368395646006 2304 56-57 1.0944343251365751 2304 58-59 0.9303578343164745 2304 60-61 0.5267224766928962 2304 62-63 0.3217333430539 2304 64-65 0.4111319238181501 2304 66-67 0.45976703756645065 2304 68-69 0.5702939491746832 2304 70-71 0.5397158670647322 2304 72-73 0.3984662443122531 2304 74-75 0.7001289184354924 2304 76-77 0.7217682080829064 2304 78-79 0.6467284406717333 2304 80-81 1.3388759021546228 2304 82-83 1.014132674247744 2304 84-85 0.6862043122579067 2304 86-87 0.6221130303986051 2304 88-89 0.4111178934027677 2304 90-91 0.4296157501375646 2304 92-93 0.3852678938420766 2304 94-95 0.19257591580027977 2304 96-97 0.2336584382536664 2304 98-99 0.6469948136633867 2304 100-101 0.5769830336710697 1102 1 1.4077311848349652 1102 2 0.017892664579353124 1102 3 0.22374498789041297 1102 4 0.0793058141198486 1102 5 0.18272642651587034 1102 6 0.10762940157925982 1102 7 -4.4831158406557705 1102 8 -0.9663600461202577 1102 9 0.21425457204728104 1102 10-11 0.6127970953645914 1102 12-13 0.8719807297135702 1102 14-15 0.704285292002865 1102 16-17 0.5374278869113098 1102 18-19 0.8243266861462928 1102 20-21 1.016660860204702 1102 22-23 0.6225419499807501 1102 24-25 0.9510153801154573 1102 26-27 0.986259789300874 1102 28-29 0.8223146349281123 1102 30-31 1.0393789017886235 1102 32-33 0.892831338310188 1102 34-35 0.8946207601739928 1102 36-37 1.0314709200535503 1102 38-39 0.7734732095577783 1102 40-41 1.0829735404082115 1102 42-43 1.0878184037569483 1102 44-45 0.9552739158512296 1102 46-47 0.7732785098797521 1102 48-49 0.7326256095132067 1102 50-51 0.6446211666690616 1102 52-53 0.813029644322441 1102 54-55 1.197540878816156 1102 56-57 0.9307439055412132 1102 58-59 0.8387145824129689 1102 60-61 0.7490604964135983 1102 62-63 0.5984920029254042 1102 64-65 0.39193568991438 1102 66-67 0.4392457755251655 1102 68-69 0.6515361439283751 1102 70-71 0.6196841332106899 1102 72-73 0.4290893666231099 1102 74-75 0.46689645710400285 1102 76-77 0.48835148868521117 1102 78-79 0.6892633582934522 1102 80-81 1.1954128559513144 1102 82-83 0.9075950881470654 1102 84-85 0.6342036321798368 1102 86-87 0.5703168733392907 1102 88-89 0.4553352392341772 1102 90-91 0.17589904386993638 1102 92-93 0.22347586939673647 1102 94-95 0.17502228276109832 1102 96-97 0.016097217566617417 1102 98-99 0.08670759465030287 1102 100-101 0.20728653802467534 2202 1 1.5887043543101953 2202 2 1.00191695475338 2202 3 0.43604038672547674 2202 4 -0.2650555268546171 2202 5 0.10011341043698962 2202 6 0.4648533035883773 2202 7 0.6326555105661527 2202 8 -3.94210561079948 2202 9 -0.2828421048769698 2202 10-11 0.5461863147268744 2202 12-13 0.06992212599022452 2202 14-15 0.17068162350354044 2202 16-17 0.22122340209123337 2202 18-19 0.7367265675572199 2202 20-21 1.2464480123913617 2202 22-23 -2.6944112760115857 2202 24-25 -2.8797398389090105 2202 26-27 -1.4169553109961512 2202 28-29 0.6108799518088119 2202 30-31 1.3157537917503532 2202 32-33 1.1860338963109243 2202 34-35 -1.8512315498578076 2202 36-37 -0.14203461379385374 2202 38-39 0.4185601239757091 2202 40-41 1.2812816381284406 2202 42-43 1.3023530741027827 2202 44-45 1.380270680299894 2202 46-47 0.6780837634364474 2202 48-49 0.9043533196861091 2202 50-51 -3.5228189950359443 2202 52-53 -0.20260415167363988 2202 54-55 1.413400159415147 2202 56-57 1.4475121103054605 2202 58-59 1.5337900648307183 2202 60-61 1.4209364935497746 2202 62-63 1.2552610210068664 2202 64-65 1.1536250071278324 2202 66-67 1.2718898478347995 2202 68-69 1.179727338267817 2202 70-71 1.4314854840259557 2202 72-73 1.2042156628052254 2202 74-75 0.7772603625587458 2202 76-77 1.3811024362756896 2202 78-79 1.3740719933598733 2202 80-81 1.8834364225767573 2202 82-83 1.711766004902362 2202 84-85 1.5221908695117037 2202 86-87 1.6209011540099958 2202 88-89 1.5352886067977067 2202 90-91 1.2851593183695549 2202 92-93 1.300239379869545 2202 94-95 1.1733128148544765 2202 96-97 1.295837688382786 2202 98-99 1.353096822280751 2202 100-101 1.3806324288407232 2305 1 1.409797719316046 2305 2 0.7168420922856882 2305 3 0.1590492368663412 2305 4 -0.11437288573338833 2305 5 0.23592084469801478 2305 6 0.3508499505520035 2305 7 0.540203929300894 2305 8 0.015628232312430157 2305 9 0.5053410262643538 2305 10-11 0.4220943835071935 2305 12-13 0.8433251655455862 2305 14-15 0.7205366454135884 2305 16-17 0.7163137013065182 2305 18-19 0.7013867452657436 2305 20-21 0.864655631159728 2305 22-23 0.6114909815473197 2305 24-25 0.832612843409585 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-0.9211026708368522 1208 42-43 -0.20996936174618952 1208 44-45 -0.1742555059341413 1208 46-47 -0.2767200316323226 1208 48-49 -1.7036561155163064 1208 50-51 -0.2379816392283587 1208 52-53 -1.7768981546909188 1208 54-55 -1.0721898376397867 1208 56-57 -6.179200765260564 1208 58-59 -2.104232140773025 1208 60-61 -1.314302578405666 1208 62-63 0.2441523304201212 1208 64-65 -0.0903750975290869 1208 66-67 0.6679404171571761 1208 68-69 0.29382686841183414 1208 70-71 -3.9529460864259214 1208 72-73 -0.6679443897884063 1208 74-75 -5.702023631758749 1208 76-77 -1.5124023745576274 1208 78-79 -3.9691367737872163 1208 80-81 0.6428727533671044 1208 82-83 0.16400852630484408 1208 84-85 -2.8912891325532257 1208 86-87 -1.033962388595807 1208 88-89 -4.723640307173742 1208 90-91 -0.3368021399555481 1208 92-93 -2.1223335739042533 1208 94-95 -4.631271588555926 1208 96-97 -3.5215099229569446 1208 98-99 -6.164640339897424 1208 100-101 -3.7978200366055646 1209 1 1.8676213473998473 1209 2 1.231440879378642 1209 3 0.592689206360081 1209 4 0.3848846519247431 1209 5 0.3684242777012088 1209 6 1.0636300589604204 1209 7 0.8911764474415236 1209 8 0.3995814469566952 1209 9 -0.08256877046199662 1209 10-11 -3.081313180324635 1209 12-13 -0.3913154889752306 1209 14-15 -1.4891936238660435 1209 16-17 -0.3834946379801494 1209 18-19 -16.873830249909695 1209 20-21 -14.054123683120828 1209 22-23 -3.657612671545742 1209 24-25 -1.8016579699263175 1209 26-27 -1.249190174406209 1209 28-29 -3.22486513142767 1209 30-31 -0.779014417575354 1209 32-33 -0.7632627904543483 1209 34-35 -3.1159723208893197 1209 36-37 -3.7724737092793426 1209 38-39 -1.2691620671586037 1209 40-41 -10.886343878822927 1209 42-43 -5.085106736963702 1209 44-45 -2.85254755038266 1209 46-47 -2.5476258685526716 1209 48-49 -3.241984755196377 1209 50-51 -2.5791606238728626 1209 52-53 -1.2057988476725718 1209 54-55 -3.0733823308473447 1209 56-57 -1.0456835017748958 1209 58-59 -1.9091164021522715 1209 60-61 -2.424990751925833 1209 62-63 -1.1431284416286012 1209 64-65 -0.4032633733174684 1209 66-67 -0.28949616128407385 1209 68-69 -1.3661713762381638 1209 70-71 -1.4260997253427945 1209 72-73 -0.7291097513905029 1209 74-75 -0.15656006671921574 1209 76-77 -16.950801037438843 1209 78-79 -16.76383523518478 1209 80-81 -22.199844070517866 1209 82-83 -9.966957755711217 1209 84-85 -3.8177561853968918 1209 86-87 -2.407941614410138 1209 88-89 -1.6204772427905567 1209 90-91 -6.378433508823274 1209 92-93 -4.100344953151438 1209 94-95 -1.2569117189097447 1209 96-97 -0.2001387274872748 1209 98-99 0.5924631036413608 1209 100-101 0.6281118252865454 1212 1 -28.624181931288675 1212 2 -16.26011090094639 1212 3 -5.8421880671615085 1212 4 -1.42424462059558 1212 5 -0.7050864505341465 1212 6 -2.553792519688905 1212 7 -0.38374078243978715 1212 8 -1.5667638213318398 1212 9 0.20447587581087134 1212 10-11 0.6297931290962779 1212 12-13 0.066088008859289 1212 14-15 -0.2658359199870617 1212 16-17 -0.20716948956069103 1212 18-19 -1.2294684181514768 1212 20-21 -0.8742557138579414 1212 22-23 -0.6270987175191749 1212 24-25 0.19971051177925148 1212 26-27 -0.8981492309449308 1212 28-29 -0.1141662965853456 1212 30-31 -0.5859917850841327 1212 32-33 0.188831494274865 1212 34-35 -1.3600054336597722 1212 36-37 -1.4811116744332438 1212 38-39 -0.4698672071711698 1212 40-41 -0.48555112764336883 1212 42-43 0.11423405394551622 1212 44-45 0.9452312349628968 1212 46-47 -2.248949127782545 1212 48-49 -1.5206974850280979 1212 50-51 -0.28998414522779115 1212 52-53 -1.1543987220414635 1212 54-55 -0.6269219636833512 1212 56-57 -1.286186781880314 1212 58-59 -0.07650273197643997 1212 60-61 0.08313040180225073 1212 62-63 -4.527644643790779 1212 64-65 -4.077476171632313 1212 66-67 -1.7382294596674015 1212 68-69 0.027102400393506088 1212 70-71 -0.8459910024821831 1212 72-73 -1.3002524985555404 1212 74-75 -0.3176683834040652 1212 76-77 0.9040383578315669 1212 78-79 -1.2838488373497903 1212 80-81 0.47278830263486427 1212 82-83 0.5994159684954425 1212 84-85 -3.3132752638502154 1212 86-87 -1.8830727950119375 1212 88-89 -0.6780739102601281 1212 90-91 -0.030513270407549697 1212 92-93 -2.3024699372113986 1212 94-95 -1.7664550781722035 1212 96-97 0.0795652365047701 1212 98-99 -0.51771797518035 1212 100-101 0.05356912724044349 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 7.0 10 17.0 11 25.0 12 83.0 13 340.0 14 1089.0 15 2406.0 16 4509.0 17 7444.0 18 11055.0 19 15913.0 20 21531.0 21 28818.0 22 37134.0 23 47206.0 24 58990.0 25 72065.0 26 88053.0 27 105970.0 28 126511.0 29 149492.0 30 176071.0 31 206135.0 32 239029.0 33 272881.0 34 307619.0 35 340512.0 36 363320.0 37 349297.0 38 148075.0 39 784.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.68877086393821 24.49193930525234 18.316995434631153 18.502294396178296 2 21.722937653131737 30.43972159837401 28.930942909692302 18.906397838801954 3 17.91794191768425 26.951526844688566 33.661347796303396 21.46918344132379 4 21.98708767572507 27.837100841946892 29.717670955063273 20.458140527264764 5 20.251905252252474 30.14284560614407 28.920901197748734 20.68434794385472 6 19.385323029537265 30.563630381848593 29.04128253414811 21.009764054466036 7 20.83090460085464 29.425836644003383 30.34011891074216 19.403139844399817 8 19.616470278441938 28.96118542465137 30.790158456529642 20.63218584037705 9 20.109433579259843 28.84017562917395 30.301720061073656 20.748670730492552 10-11 20.748733576400355 29.123516404510198 29.379000731212134 20.748749287877306 12-13 20.658281096672372 29.231751956375895 29.230461190608644 20.879505756343093 14-15 20.39886110610089 29.617918756555618 29.329283122789 20.653937014554486 16-17 20.662372915335443 29.430992844498444 29.198540776052113 20.708093464114004 18-19 20.824984148411453 29.464465622540764 28.905133806912158 20.80541642213563 20-21 20.58598808975217 29.53550096303543 29.089294807307287 20.78921613990511 22-23 20.66468042143844 29.324910723446067 29.156122300041183 20.854286555074314 24-25 20.684112271700457 29.130413742891736 29.2726497437301 20.91282424167771 26-27 20.640670037930647 29.397618831399196 29.197438903565153 20.764272227105 28-29 20.985756917684746 29.27021446480268 29.051840646659254 20.692187970853322 30-31 20.90146484384206 29.173431766783864 28.969701045158867 20.95540234421521 32-33 20.67196730001725 29.31722320384039 29.1355985302856 20.87521096585676 34-35 20.961089898871506 29.2371732287745 29.011729246003075 20.790007626350913 36-37 20.939360926248128 29.212694747684676 29.08818129284716 20.759763033220032 38-39 20.724286518264435 29.260504772046897 29.253701702527064 20.761507007161605 40-41 21.008376558958474 29.167704951053203 28.99306892144851 20.83084956853981 42-43 20.81868107178668 29.1930868244497 29.135551395854748 20.852680707908874 44-45 20.69869252231108 29.191437119369834 29.304261235355728 20.80560912296336 46-47 20.920582048181753 29.186531814671646 29.156987498039545 20.73589863910706 48-49 20.925610823329045 29.104806615164414 29.12741544470578 20.842167116800763 50-51 20.807555345719756 29.231401316466517 29.149685809295395 20.811357528518336 52-53 20.966282033576405 29.140532559950742 29.137233146162302 20.75595226031055 54-55 20.72732774715809 29.056517091851685 29.308094517821193 20.908060643169033 56-57 20.68464421923623 29.267403629845845 29.256044060673613 20.791908090244313 58-59 20.899894964970763 29.082310883871582 29.26435692346596 20.75343722769169 60-61 20.899136337776163 29.045833064268667 29.09208861898102 20.962941978974147 62-63 20.78101395593648 29.159650728434684 29.238445010939284 20.820890304689556 64-65 20.927845806926822 29.138081435542134 29.130115488942575 20.803957268588466 66-67 20.88442885023087 29.109215552782175 29.180470515293578 20.825885081693382 68-69 20.77363250798224 29.24505726581258 29.19861172454526 20.782698501659922 70-71 21.030459322861848 29.07899903229167 29.015333596982394 20.87520804786409 72-73 20.836414647276328 29.10999603129384 29.171389165039237 20.8822001563906 74-75 20.697265115068376 29.162816278688503 29.329087021466655 20.810831584776466 76-77 20.88462640015359 29.1655415081216 29.2037968986539 20.74603519307091 78-79 20.92047277261907 29.108994208885786 29.127031049604295 20.84350196889085 80-81 20.79324835842983 29.116823923510005 29.240932668567677 20.84899504949249 82-83 20.991156988785068 29.110964777692878 29.04563324372638 20.85224498979568 84-85 20.822534377407422 29.01400236030675 29.20506433650665 20.95839892577918 86-87 20.692573910972158 29.212553811499248 29.280274831066695 20.8145974464619 88-89 20.896346246188113 29.082755946290433 29.099112801651213 20.92178500587024 90-91 20.919877365190263 28.974461767612723 29.062120920914563 21.043539946282447 92-93 20.729770745751196 29.092511564220512 29.2295474594227 20.948170230605594 94-95 20.94529265265273 29.06896767925237 28.990288360410677 20.99545130768422 96-97 20.881745672587396 29.04539226408216 29.079458460610176 20.993403602720267 98-99 20.736141003785217 29.07473965547612 29.16974323107381 21.01937610966486 100-101 20.983893524411133 28.866748377567212 28.96646710350571 21.18289099451594 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3.0 1 3.5 2 9.5 3 25.0 4 53.5 5 110.5 6 202.0 7 380.5 8 693.5 9 1196.0 10 2043.5 11 3390.0 12 5564.5 13 8455.5 14 12077.5 15 16584.0 16 21797.5 17 27246.0 18 32709.5 19 38218.5 20 43039.0 21 47680.5 22 52128.5 23 55956.5 24 59394.5 25 62183.5 26 64780.0 27 67330.5 28 69743.0 29 72002.5 30 74593.0 31 77120.5 32 78747.5 33 80237.5 34 81804.0 35 82646.5 36 82970.5 37 83673.0 38 83488.0 39 81383.5 40 78965.0 41 75927.5 42 71612.0 43 67467.0 44 63816.0 45 60275.0 46 56972.0 47 54284.5 48 51890.5 49 49238.5 50 46590.5 51 44165.0 52 42061.0 53 40220.0 54 38893.0 55 38110.0 56 37332.5 57 37075.0 58 37149.0 59 37147.5 60 37068.0 61 37022.5 62 36994.0 63 36966.0 64 37178.5 65 37288.0 66 37344.5 67 37340.0 68 36839.5 69 35713.0 70 34169.0 71 32007.0 72 29165.0 73 26108.0 74 22472.0 75 18399.5 76 14633.0 77 11036.5 78 7886.5 79 5505.5 80 3629.5 81 2280.5 82 1479.0 83 982.5 84 654.5 85 423.0 86 289.5 87 195.0 88 137.5 89 104.0 90 77.0 91 55.0 92 37.0 93 23.5 94 11.5 95 8.0 96 5.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7280069960064568 2 0.002450990404372567 3 2.1996067731548677E-4 4 3.14229539022124E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.1878464184274257 14-15 3.14229539022124E-5 16-17 3.2994101597323016E-4 18-19 0.11574645069879937 20-21 4.71344308533186E-5 22-23 1.57114769511062E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0016968395107194694 42-43 0.0 44-45 0.0 46-47 0.0228130645330062 48-49 6.28459078044248E-5 50-51 1.414032925599558E-4 52-53 1.0998033865774338E-4 54-55 0.0020896264344971243 56-57 0.0015083017873061952 58-59 0.09337330752042414 60-61 0.0020896264344971243 62-63 0.0015554362181595138 64-65 0.002859488805101328 66-67 0.00479200047008739 68-69 0.005200498870816151 70-71 0.003975003668629868 72-73 0.039262980900814384 74-75 0.04495053555711483 76-77 0.1589058778834881 78-79 3.6136396987544256E-4 80-81 0.11698765737793676 82-83 0.00491769228569624 84-85 0.005451882502033851 86-87 0.006724512135073453 88-89 0.0073843941670199125 90-91 0.023425812134099343 92-93 0.011752184759427436 94-95 0.015192998211719693 96-97 0.010903765004067702 98-99 0.012616315991738276 100-101 0.013731830855266816 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3182387.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.56114450694994 #Duplication Level Percentage of deduplicated Percentage of total 1 98.38700087994641 95.98748410453865 2 1.2374764673884455 2.4145924091766817 3 0.14979675729391628 0.43843029255072824 4 0.09963297143073019 0.3888122689364114 5 0.06950894049961895 0.33906858943041557 6 0.03436852870638674 0.2011819797369033 7 0.009217417157582277 0.06294832371042071 8 0.004845218732073153 0.03781640679100557 9 0.0028748435687774468 0.025242572596353496 >10 0.0052442988540398775 0.0636170149130331 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 3.367642202149134E-5 0.040806037619421594 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.426886170663718E-5 0.0 0.0 2 9.426886170663718E-5 0.0 0.0 3 1.5711476951106197E-4 0.0 0.0 4 1.5711476951106197E-4 0.0 0.0 5 1.8853772341327437E-4 0.0 0.0 6 1.8853772341327437E-4 0.0 0.0 7 2.1996067731548677E-4 0.0 0.0 8 2.513836312176992E-4 0.0 0.0 9 2.8280658511991154E-4 0.0 0.0 10-11 3.2994101597323016E-4 3.14229539022124E-5 0.0 12-13 4.3992135463097353E-4 3.14229539022124E-5 0.0 14-15 4.7134430853318593E-4 3.14229539022124E-5 0.0 16-17 5.027672624353984E-4 4.71344308533186E-5 0.0 18-19 5.027672624353984E-4 6.28459078044248E-5 0.0 20-21 5.027672624353984E-4 6.28459078044248E-5 0.0 22-23 5.656131702398231E-4 6.28459078044248E-5 0.0 24-25 5.813246471909294E-4 6.28459078044248E-5 1.57114769511062E-5 26-27 6.127476010931417E-4 6.28459078044248E-5 3.14229539022124E-5 28-29 7.855738475553099E-4 6.28459078044248E-5 3.14229539022124E-5 30-31 0.00111551486352854 6.28459078044248E-5 3.14229539022124E-5 32-33 0.0015083017873061952 6.28459078044248E-5 3.14229539022124E-5 34-35 0.002152472342301549 6.28459078044248E-5 3.14229539022124E-5 36-37 0.002906623235954647 6.28459078044248E-5 3.14229539022124E-5 38-39 0.003849311853021018 6.28459078044248E-5 3.14229539022124E-5 40-41 0.004980538193500665 9.426886170663718E-5 6.28459078044248E-5 42-43 0.006834492473731196 1.256918156088496E-4 6.28459078044248E-5 44-45 0.00925405992420155 1.256918156088496E-4 1.099803386577434E-4 46-47 0.012584893037836064 1.5711476951106197E-4 1.256918156088496E-4 48-49 0.016575608183417037 1.5711476951106197E-4 1.256918156088496E-4 50-51 0.02174468410033098 1.5711476951106197E-4 1.256918156088496E-4 52-53 0.028736291343573236 1.5711476951106197E-4 1.256918156088496E-4 54-55 0.036654875726930755 1.5711476951106197E-4 1.256918156088496E-4 56-57 0.0453747454347947 1.5711476951106197E-4 1.256918156088496E-4 58-59 0.056388490777520145 1.5711476951106197E-4 1.256918156088496E-4 60-61 0.06905194120011174 1.5711476951106197E-4 1.4140329255995577E-4 62-63 0.08342794261037391 2.0424920036438057E-4 1.5711476951106197E-4 64-65 0.10001926227074207 2.3567215426659297E-4 1.5711476951106197E-4 66-67 0.11868449688865622 2.513836312176992E-4 1.5711476951106197E-4 68-69 0.13901514806338763 2.513836312176992E-4 1.5711476951106197E-4 70-71 0.16064985182506086 2.513836312176992E-4 1.8853772341327437E-4 72-73 0.18641667402487505 2.513836312176992E-4 2.0424920036438057E-4 74-75 0.2159385392160036 2.513836312176992E-4 2.1996067731548677E-4 76-77 0.2504095196467306 2.513836312176992E-4 2.1996067731548677E-4 78-79 0.28882408079218524 3.1422953902212394E-4 2.3567215426659297E-4 80-81 0.3300352848349368 3.1422953902212394E-4 2.513836312176992E-4 82-83 0.37531576140802486 3.1422953902212394E-4 2.513836312176992E-4 84-85 0.4236599759865786 3.4565249292433633E-4 2.513836312176992E-4 86-87 0.4813682308279917 3.4565249292433633E-4 2.8280658511991154E-4 88-89 0.5464608798364248 3.613639698754425E-4 2.8280658511991154E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATGC 820 0.0 13.893352 44-45 TCTCGTA 1005 0.0 12.278111 40-41 TATGCCG 930 0.0 11.994842 46-47 CTCGTAT 1080 0.0 11.867237 42-43 TCGTATG 1105 0.0 11.383955 42-43 GAGCACA 3190 0.0 10.302069 9 GCCGTCT 1415 0.0 10.228126 48-49 AGAGCAC 3080 0.0 10.051449 8 GTATGCC 1195 0.0 9.930742 44-45 CCGTCTT 1370 0.0 9.52507 50-51 ATCTCGT 1585 0.0 9.282324 40-41 TCGGAAG 3220 0.0 8.841533 3 AGTCACC 1750 0.0 8.67649 28-29 GGCATCT 1730 0.0 8.639795 36-37 ATGCCGT 1410 0.0 8.58481 46-47 CGGAAGA 3355 0.0 8.485764 4 CAGTCAC 1950 0.0 8.394922 26-27 GAAGAGC 3860 0.0 8.390496 6 ATCGGAA 3595 0.0 8.315222 2 CATCTCG 1770 0.0 8.310505 38-39 >>END_MODULE