FastQCFastQC Report
Thu 11 Sep 2014
LONLib34_CATGGC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib34_CATGGC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3182387
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC8200.013.89335244-45
TCTCGTA10050.012.27811140-41
TATGCCG9300.011.99484246-47
CTCGTAT10800.011.86723742-43
TCGTATG11050.011.38395542-43
GAGCACA31900.010.3020699
GCCGTCT14150.010.22812648-49
AGAGCAC30800.010.0514498
GTATGCC11950.09.93074244-45
CCGTCTT13700.09.5250750-51
ATCTCGT15850.09.28232440-41
TCGGAAG32200.08.8415333
AGTCACC17500.08.6764928-29
GGCATCT17300.08.63979536-37
ATGCCGT14100.08.5848146-47
CGGAAGA33550.08.4857644
CAGTCAC19500.08.39492226-27
GAAGAGC38600.08.3904966
ATCGGAA35950.08.3152222
CATCTCG17700.08.31050538-39
Produced by FastQC (version 0.11.2)