##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib33_CAGGCG_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2720035 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.850528761578435 27.0 22.0 33.0 2.0 33.0 2 24.883168782754634 27.0 15.0 33.0 2.0 33.0 3 25.75033997724294 33.0 22.0 33.0 6.0 33.0 4 27.84400421318108 33.0 22.0 37.0 2.0 37.0 5 27.904722181883688 33.0 15.0 37.0 6.0 37.0 6 28.975006571606617 33.0 22.0 37.0 15.0 37.0 7 28.649569950386667 33.0 27.0 37.0 6.0 37.0 8 28.406064995487192 33.0 22.0 37.0 6.0 37.0 9 29.13837321946225 33.0 27.0 37.0 15.0 37.0 10-11 28.743201098515275 33.0 24.5 37.0 10.5 37.0 12-13 29.028522978564617 33.0 27.0 37.0 10.5 37.0 14-15 29.32841783285877 33.0 27.0 37.0 6.0 40.0 16-17 30.157529406790722 33.0 27.0 37.0 15.0 40.0 18-19 30.44845654559592 33.0 27.0 37.0 15.0 40.0 20-21 30.437955026314 33.0 27.0 37.0 15.0 40.0 22-23 30.42199898163075 33.0 27.0 37.0 15.0 40.0 24-25 29.9996628719851 33.0 27.0 37.0 10.5 40.0 26-27 29.47669552046205 33.0 27.0 37.0 10.5 40.0 28-29 29.620626021356344 33.0 27.0 37.0 15.0 40.0 30-31 29.49510098215648 33.0 27.0 37.0 15.0 40.0 32-33 29.315872222232436 33.0 27.0 37.0 10.5 40.0 34-35 29.335519947353617 33.0 24.5 37.0 10.5 40.0 36-37 29.571004049580246 33.0 27.0 37.0 6.0 40.0 38-39 29.565404121638142 33.0 27.0 37.0 15.0 40.0 40-41 29.6041773359534 33.0 27.0 37.0 15.0 40.0 42-43 29.47045093169757 33.0 27.0 37.0 15.0 40.0 44-45 29.189072199438613 33.0 24.5 37.0 10.5 40.0 46-47 28.799574821647518 33.0 22.0 37.0 10.5 40.0 48-49 28.610686259551805 33.0 22.0 37.0 10.5 40.0 50-51 27.81140702233611 33.0 22.0 37.0 10.5 38.5 52-53 27.530358249066648 33.0 22.0 37.0 6.0 37.0 54-55 28.450545856946693 33.0 22.0 37.0 10.5 40.0 56-57 28.33698353146191 33.0 22.0 37.0 6.0 40.0 58-59 28.508736468464562 33.0 22.0 37.0 10.5 40.0 60-61 28.25459433426408 33.0 22.0 37.0 10.5 40.0 62-63 28.093549531531764 33.0 22.0 37.0 10.5 40.0 64-65 28.03458999608461 33.0 22.0 37.0 6.0 40.0 66-67 27.899807723062388 33.0 22.0 37.0 10.5 38.5 68-69 27.607050276926586 33.0 22.0 37.0 6.0 37.0 70-71 27.499431441139542 33.0 22.0 37.0 6.0 37.0 72-73 27.257466172310284 27.0 22.0 37.0 6.0 37.0 74-75 27.20048271437684 27.0 22.0 33.0 6.0 37.0 76-77 26.814714332719984 27.0 22.0 33.0 6.0 37.0 78-79 26.26679307435382 27.0 22.0 33.0 6.0 37.0 80-81 26.13590707472514 27.0 22.0 33.0 6.0 37.0 82-83 25.710710523945465 27.0 22.0 33.0 6.0 37.0 84-85 25.80230750707252 27.0 22.0 33.0 6.0 37.0 86-87 25.502551621578398 27.0 22.0 33.0 6.0 37.0 88-89 25.141682368057765 27.0 15.0 33.0 6.0 37.0 90-91 25.003697195072856 27.0 15.0 33.0 6.0 37.0 92-93 24.889411900949803 27.0 15.0 33.0 6.0 37.0 94-95 24.670439534785398 27.0 15.0 33.0 6.0 37.0 96-97 24.536036668645806 27.0 15.0 33.0 6.0 37.0 98-99 24.28544044470016 27.0 15.0 33.0 6.0 37.0 100-101 23.79800940061433 27.0 15.0 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.231437447196075 2306 2 8.525986694738979 2306 3 7.482052611223381 2306 4 9.100724722775144 2306 5 8.905962579252659 2306 6 7.97851380983483 2306 7 8.14611299844228 2306 8 8.543332964638697 2306 9 7.438909233720487 2306 10-11 7.735376778673899 2306 12-13 7.417230696693554 2306 14-15 7.982049733174769 2306 16-17 6.708217643767817 2306 18-19 6.232764769706375 2306 20-21 6.080414397129861 2306 22-23 5.662996604944983 2306 24-25 5.6535823015946285 2306 26-27 6.312077880824017 2306 28-29 5.420434027258899 2306 30-31 5.3481603314369295 2306 32-33 5.210685914491737 2306 34-35 4.591912305916413 2306 36-37 4.650687189020882 2306 38-39 4.332209211163416 2306 40-41 3.531809425451371 2306 42-43 3.2766882120723757 2306 44-45 2.9471523115575735 2306 46-47 3.298864923217497 2306 48-49 3.0230918900798187 2306 50-51 2.921483559238723 2306 52-53 3.4189086103750554 2306 54-55 2.97459959990109 2306 56-57 3.26372754813557 2306 58-59 2.907911535303647 2306 60-61 2.819771517148606 2306 62-63 2.256364498805315 2306 64-65 1.8015596660009194 2306 66-67 1.693273414058556 2306 68-69 1.9189213349380374 2306 70-71 1.9135030913817985 2306 72-73 0.9708790134838949 2306 74-75 0.7136226242251773 2306 76-77 1.1729758624339226 2306 78-79 1.3814198688275319 2306 80-81 1.0461670317983582 2306 82-83 1.2194114167044567 2306 84-85 0.7120213827381185 2306 86-87 0.7673869011478018 2306 88-89 0.565836449683264 2306 90-91 0.5616967634366858 2306 92-93 0.6078162404271232 2306 94-95 0.8174394222241013 2306 96-97 0.41328192692363075 2306 98-99 0.21997269772550254 2306 100-101 0.0955353200094855 2307 1 8.33511926298345 2307 2 8.428230408690922 2307 3 7.480442644883841 2307 4 9.115402774275065 2307 5 9.063897972854392 2307 6 7.600656915378302 2307 7 8.130847170118596 2307 8 8.472063713306692 2307 9 7.529768116252953 2307 10-11 7.8148585089794445 2307 12-13 7.205447505786896 2307 14-15 8.134007798191824 2307 16-17 6.745793037042169 2307 18-19 6.418189036031759 2307 20-21 6.313149850209637 2307 22-23 5.96651839361019 2307 24-25 6.0811463815177795 2307 26-27 6.8220108587209545 2307 28-29 5.950165987734238 2307 30-31 5.878512326288465 2307 32-33 5.908615619558564 2307 34-35 5.591894192799494 2307 36-37 5.464235157800292 2307 38-39 5.007612131201107 2307 40-41 4.349952811466643 2307 42-43 4.252352422404467 2307 44-45 4.180304833230281 2307 46-47 4.502637323814934 2307 48-49 4.221675068066968 2307 50-51 4.053579266366846 2307 52-53 4.608094857105975 2307 54-55 4.090629645208875 2307 56-57 4.41716529962844 2307 58-59 4.143957610239273 2307 60-61 4.1896099173849315 2307 62-63 3.758209001857466 2307 64-65 3.6410241818418356 2307 66-67 3.369967364013842 2307 68-69 3.386787841568424 2307 70-71 3.0659990981192777 2307 72-73 2.480175313261114 2307 74-75 2.189569379955831 2307 76-77 2.399566958997898 2307 78-79 2.7584498081756443 2307 80-81 2.493028131629014 2307 82-83 2.627804169941623 2307 84-85 1.8156329295192002 2307 86-87 1.865139245979364 2307 88-89 2.1582953974504164 2307 90-91 1.7598003077356026 2307 92-93 1.5581518041136384 2307 94-95 1.771663826796921 2307 96-97 1.6222802295364396 2307 98-99 1.431282365889352 2307 100-101 1.0828451796587792 1101 1 8.053235928458687 1101 2 7.8567655376524606 1101 3 7.270813551219145 1101 4 8.768576431444 1101 5 8.559759425474354 1101 6 7.3627839480896355 1101 7 7.257375400474313 1101 8 8.108228692316796 1101 9 7.538346684256737 1101 10-11 7.567094934897671 1101 12-13 7.306677415905053 1101 14-15 7.850726155028731 1101 16-17 7.060854773647897 1101 18-19 6.13623559406734 1101 20-21 5.924029267298348 1101 22-23 5.365121092133325 1101 24-25 6.067563233883352 1101 26-27 6.676437360030842 1101 28-29 5.982569016161818 1101 30-31 5.712452938591433 1101 32-33 5.888236790688712 1101 34-35 5.579188386882258 1101 36-37 5.329933933871249 1101 38-39 5.120769589950456 1101 40-41 4.671065295561995 1101 42-43 4.3459319144366795 1101 44-45 4.430570556462371 1101 46-47 4.639256664865204 1101 48-49 4.31138651152823 1101 50-51 3.9328289496525457 1101 52-53 4.806433994418178 1101 54-55 4.563850052111665 1101 56-57 4.7832984070425155 1101 58-59 4.345765391900624 1101 60-61 4.597016177591062 1101 62-63 4.1215632579853185 1101 64-65 3.8943303932737763 1101 66-67 3.4759860736414048 1101 68-69 3.6680778651587893 1101 70-71 3.5990933262598546 1101 72-73 3.127468413532789 1101 74-75 2.459014551872805 1101 76-77 2.7952902448210644 1101 78-79 3.379196537932252 1101 80-81 2.9289033576038292 1101 82-83 3.1468261722838093 1101 84-85 2.337333193600873 1101 86-87 2.5465571003017864 1101 88-89 2.570443601652009 1101 90-91 2.3424678806225145 1101 92-93 1.956428244218774 1101 94-95 2.3109482999148945 1101 96-97 2.1446409439605105 1101 98-99 2.027608384389108 1101 100-101 1.6722896550026398 2201 1 6.965104573323295 2201 2 7.82395073940274 2201 3 6.367623600320563 2201 4 8.463538486882388 2201 5 3.867201983023378 2201 6 5.565111812848837 2201 7 6.6087471251436405 2201 8 7.701164712184831 2201 9 6.502342284661573 2201 10-11 4.1024986998919 2201 12-13 6.396188624951673 2201 14-15 7.025460238162076 2201 16-17 5.883094469737877 2201 18-19 1.9254338223274168 2201 20-21 4.934370612190694 2201 22-23 4.997116492355037 2201 24-25 5.370054683827785 2201 26-27 6.125092550953216 2201 28-29 4.909107809469717 2201 30-31 5.130350323601448 2201 32-33 5.198833141700657 2201 34-35 4.796674936120098 2201 36-37 4.868760335636889 2201 38-39 4.347854101071832 2201 40-41 4.069764014375856 2201 42-43 3.950441624525549 2201 44-45 3.595605406768229 2201 46-47 4.025784327037048 2201 48-49 3.847305668469719 2201 50-51 3.3573605406311557 2201 52-53 4.3300985714107085 2201 54-55 3.9739102426044823 2201 56-57 4.060242311124227 2201 58-59 3.657219611679121 2201 60-61 3.7267031771954393 2201 62-63 3.5265711267675 2201 64-65 3.400764956087805 2201 66-67 2.9813219028409854 2201 68-69 3.1861432402919725 2201 70-71 3.069258596404893 2201 72-73 2.492275113630946 2201 74-75 2.1834504732833686 2201 76-77 2.414168890855688 2201 78-79 2.830893935293151 2201 80-81 2.2807744743490943 2201 82-83 2.63842668033514 2201 84-85 2.1532846702824244 2201 86-87 2.131690980432854 2201 88-89 2.14321211054045 2201 90-91 1.9749353338860516 2201 92-93 1.8045139562399015 2201 94-95 1.998792799808509 2201 96-97 1.8312022285972809 2201 98-99 1.7908422317730128 2201 100-101 1.4094342912532376 2304 1 1.574011229758856 2304 2 4.813214990724379 2304 3 5.732736541980071 2304 4 4.273285808206431 2304 5 6.479376832707867 2304 6 6.296172724178049 2304 7 7.0538361182940115 2304 8 6.511425856644568 2304 9 5.785485633765703 2304 10-11 6.795815066769844 2304 12-13 6.569466506304803 2304 14-15 6.797619561760605 2304 16-17 5.549278257537665 2304 18-19 5.313851760668339 2304 20-21 5.009708294094029 2304 22-23 4.616634403946957 2304 24-25 4.7352898388396945 2304 26-27 5.673152919078742 2304 28-29 5.091055381567557 2304 30-31 5.09609899897243 2304 32-33 5.016536778414167 2304 34-35 4.740123309865766 2304 36-37 4.830895390754943 2304 38-39 4.519829276678536 2304 40-41 3.8210827560691243 2304 42-43 3.359638468053138 2304 44-45 3.102681258964793 2304 46-47 4.0617804673453755 2304 48-49 3.8077019447509173 2304 50-51 3.4537596631620886 2304 52-53 4.070939522628684 2304 54-55 3.6885396163622595 2304 56-57 4.510704318340331 2304 58-59 3.7413674577262555 2304 60-61 3.8047041532575463 2304 62-63 3.486717986136661 2304 64-65 3.369495607353585 2304 66-67 3.2138379174813174 2304 68-69 3.2343306063880775 2304 70-71 3.3542920192056016 2304 72-73 3.0232885413628026 2304 74-75 2.44550279562489 2304 76-77 2.721798999949467 2304 78-79 2.9592074702752136 2304 80-81 2.679361950401706 2304 82-83 2.9788426939138866 2304 84-85 2.485634845571397 2304 86-87 2.3356763356137513 2304 88-89 2.4938428540446935 2304 90-91 2.410361454223086 2304 92-93 2.424502741543467 2304 94-95 2.521118706617006 2304 96-97 2.4085407032520365 2304 98-99 2.2759796377833013 2304 100-101 1.9412916247047924 1102 1 7.012909495396492 1102 2 7.371542039401252 1102 3 6.0141577678818905 1102 4 6.050773729327709 1102 5 6.41961591895544 1102 6 5.7348084201929055 1102 7 6.696145219952651 1102 8 6.25308295406964 1102 9 4.694207153792828 1102 10-11 5.871567543814653 1102 12-13 5.771201739108651 1102 14-15 6.102111082629442 1102 16-17 5.307408493084672 1102 18-19 5.229580221930913 1102 20-21 5.4505094922971615 1102 22-23 5.105725426279999 1102 24-25 1.6044961518010474 1102 26-27 5.200951241945912 1102 28-29 4.716582041976039 1102 30-31 4.777536890059189 1102 32-33 4.880384541442677 1102 34-35 4.832479407007902 1102 36-37 4.792503013200953 1102 38-39 3.806723984823144 1102 40-41 3.853090939587627 1102 42-43 4.0429085153748225 1102 44-45 -3.72777862577162 1102 46-47 1.0657767313692297 1102 48-49 -0.7655418388477706 1102 50-51 2.5267844838085267 1102 52-53 -0.4488276092997481 1102 54-55 2.3980149909317277 1102 56-57 -0.717130478633436 1102 58-59 2.573233192787672 1102 60-61 3.6641023879648706 1102 62-63 3.35581988816498 1102 64-65 3.0124929507270437 1102 66-67 2.9815480145889808 1102 68-69 3.3346952037886446 1102 70-71 3.058774683525847 1102 72-73 2.543561037418762 1102 74-75 2.3597664614990883 1102 76-77 2.6077459644350434 1102 78-79 -1.6201885722673168 1102 80-81 1.379843195645961 1102 82-83 2.321849130461281 1102 84-85 2.203200736433704 1102 86-87 2.1207870576827474 1102 88-89 2.113843552089456 1102 90-91 1.9013666488254586 1102 92-93 2.0416602170620415 1102 94-95 2.286072625521445 1102 96-97 2.165657675272442 1102 98-99 2.0348588347985768 1102 100-101 1.6779027711782426 2202 1 -20.51160218595165 2202 2 -7.598886246184048 2202 3 -0.8176896159161942 2202 4 1.4599507794711712 2202 5 0.734593903871712 2202 6 1.3523546797446677 2202 7 3.8056737459977406 2202 8 2.495723547880285 2202 9 2.86682300058672 2202 10-11 1.8439724673476654 2202 12-13 1.1588092143839397 2202 14-15 2.4180127451847895 2202 16-17 1.4747651043211754 2202 18-19 1.4335575695648117 2202 20-21 0.8175900950391402 2202 22-23 1.1984706652231658 2202 24-25 2.129734240292496 2202 26-27 3.290119645031478 2202 28-29 -1.207771266376902 2202 30-31 0.9149021759535216 2202 32-33 1.4465980832008611 2202 34-35 1.3722501463538919 2202 36-37 2.888295629355021 2202 38-39 2.3309247529659807 2202 40-41 1.6434589184070738 2202 42-43 0.5340283300725766 2202 44-45 0.4342271411416405 2202 46-47 -1.6687288062346148 2202 48-49 0.5114716151058403 2202 50-51 1.5594946782012684 2202 52-53 1.5156465665042589 2202 54-55 -1.093333604181261 2202 56-57 0.7254785270579447 2202 58-59 1.630401961664301 2202 60-61 1.8488329247936228 2202 62-63 -2.405915739838754 2202 64-65 -0.2801879941804586 2202 66-67 0.4135117504896719 2202 68-69 1.1804096431425393 2202 70-71 -2.6507377379604016 2202 72-73 -4.698721240307734 2202 74-75 -1.3313523550012185 2202 76-77 -0.4391656586588475 2202 78-79 1.5183898685261852 2202 80-81 1.3998260735697272 2202 82-83 -0.9487895447862655 2202 84-85 -0.5105148553092427 2202 86-87 0.3487199940861636 2202 88-89 0.9849590345182833 2202 90-91 1.2017320350915313 2202 92-93 1.1673935816875591 2202 94-95 1.5341595824111813 2202 96-97 1.09861864262421 2202 98-99 1.0829654428380877 2202 100-101 0.8487990043674749 2305 1 5.503289510799252 2305 2 6.889341715722068 2305 3 6.520028514666876 2305 4 7.575225524495888 2305 5 7.680426886716106 2305 6 5.978599096130722 2305 7 2.8169313241466547 2305 8 5.810818502920863 2305 9 6.257149430082947 2305 10-11 4.5868038755934215 2305 12-13 5.143355633283111 2305 14-15 5.554336040009147 2305 16-17 4.921796108933755 2305 18-19 4.23811685429855 2305 20-21 4.951780971819371 2305 22-23 4.908725084418915 2305 24-25 5.230316281369507 2305 26-27 5.9240403111021 2305 28-29 4.997488495787813 2305 30-31 5.311522772475577 2305 32-33 5.335440697914809 2305 34-35 5.144761725496608 2305 36-37 4.431794811162089 2305 38-39 4.393994956911513 2305 40-41 3.976811293768151 2305 42-43 3.816677322919137 2305 44-45 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1209 38-39 -25.758193671247327 1209 40-41 -22.601674090307263 1209 42-43 -23.039795801830163 1209 44-45 -25.736683260011375 1209 46-47 -19.38228089874269 1209 48-49 -19.08081689044571 1209 50-51 -21.26798371111466 1209 52-53 -23.791703901907944 1209 54-55 -20.92439035078494 1209 56-57 -24.592561348032135 1209 58-59 -24.84413563141761 1209 60-61 -24.584748626971933 1209 62-63 -24.71915138720844 1209 64-65 -24.58813899105771 1209 66-67 -12.523310569818868 1209 68-69 -24.2481274695166 1209 70-71 -24.071347874880892 1209 72-73 -14.523437824234925 1209 74-75 -15.508084963029615 1209 76-77 -12.812366992499294 1209 78-79 -13.858081009179529 1209 80-81 -23.133082886314973 1209 82-83 -15.027343255384926 1209 84-85 -12.600719443680344 1209 86-87 -12.65459500644764 1209 88-89 -9.934323058723768 1209 90-91 -12.52847012614369 1209 92-93 -16.586375151464278 1209 94-95 -21.815708301326506 1209 96-97 -17.75386383362155 1209 98-99 -9.275186605964858 1209 100-101 -5.19130941604249 1212 1 -20.51160218595165 1212 2 -20.32072146117324 1212 3 -21.22004368749919 1212 4 -23.168682417742378 1212 5 -23.35116156093732 1212 6 -24.41896408076458 1212 7 -23.986543405095222 1212 8 -23.617656226321014 1212 9 -24.538653819014527 1212 10-11 -24.225808940291056 1212 12-13 -24.510299601373926 1212 14-15 -24.795054754214682 1212 16-17 -25.795712916337003 1212 18-19 -20.520909042518863 1212 20-21 -26.162952782693264 1212 22-23 -26.318824554570252 1212 24-25 -22.381562893193852 1212 26-27 -25.046176295396098 1212 28-29 -19.82350863135131 1212 30-31 -14.880428258669124 1212 32-33 -25.102284933854147 1212 34-35 -21.25792962827397 1212 36-37 -25.500966921689898 1212 38-39 -25.758193671247327 1212 40-41 -20.257160085806092 1212 42-43 -16.670480072393737 1212 44-45 -13.540789808291063 1212 46-47 -16.928822549994386 1212 48-49 -19.554514132707396 1212 50-51 -11.789002987535921 1212 52-53 -4.887153402462882 1212 54-55 -3.266211552536081 1212 56-57 -3.803993090540459 1212 58-59 -2.453458605890418 1212 60-61 -13.443239193009669 1212 62-63 -11.243568701303891 1212 64-65 -15.493799368416202 1212 66-67 -11.855200676490467 1212 68-69 -7.548349445099287 1212 70-71 -14.698428895968572 1212 72-73 -11.028297393848932 1212 74-75 -7.818513576298823 1212 76-77 -18.209901753258944 1212 78-79 -9.374681006096534 1212 80-81 -7.290130611065251 1212 82-83 -4.9876400706519135 1212 84-85 -4.260727806302121 1212 86-87 -3.0405646851996444 1212 88-89 -2.7591386571329437 1212 90-91 -3.1711337560610673 1212 92-93 -3.10938392258279 1212 94-95 -2.759659466698313 1212 96-97 -12.353740321166255 1212 98-99 -9.64788303123308 1212 100-101 -5.805803149856768 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 440.0 3 905.0 4 503.0 5 2810.0 6 3336.0 7 3462.0 8 6797.0 9 12993.0 10 17849.0 11 23064.0 12 31106.0 13 38879.0 14 43566.0 15 48311.0 16 52082.0 17 57394.0 18 63513.0 19 70116.0 20 75439.0 21 82940.0 22 90171.0 23 96189.0 24 100571.0 25 102987.0 26 107420.0 27 110652.0 28 111887.0 29 113715.0 30 116550.0 31 122728.0 32 131831.0 33 148099.0 34 164755.0 35 176162.0 36 177980.0 37 157573.0 38 55097.0 39 163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.08634104010677 26.56067996698613 14.19520195537186 21.157777037535247 2 26.36366892745668 27.77255571379576 25.24348473750585 20.62029062124171 3 20.760275451415325 25.253000371749025 28.378857707785787 25.607866469049867 4 23.877924340467892 25.16778662684586 27.292226646756262 23.662062385929982 5 22.28557708410257 26.83207531561415 26.76363592341729 24.118711676865992 6 22.32852578504867 27.327913736840966 25.930971808594705 24.41258866951566 7 24.197559541406903 27.383889789513695 26.64975423230489 21.768796436774515 8 22.41866176568249 26.820217619016383 27.27090499671416 23.490215618586962 9 23.004745453501847 26.19408113655085 27.22038823053069 23.580785179416612 10-11 23.706796289871235 26.637239599037933 26.106236622131863 23.549727488958975 12-13 23.485878046453397 26.657121713856203 26.154256676618857 23.702743563071543 14-15 23.464013093704295 26.826696432282237 26.13531876322284 23.573971710790627 16-17 23.742564516926063 26.837920897663537 25.992004439128152 23.42751014628225 18-19 23.8022842785747 26.874596531000055 25.802050910589752 23.521068279835493 20-21 23.686948034516472 26.874809487426692 25.961391093682835 23.476851384374 22-23 23.63814824283887 26.744790092253538 26.136576633057494 23.48048503185009 24-25 23.5709452949811 26.727184779778245 26.19112699007793 23.510742935162725 26-27 23.459014214777515 26.772119989191683 26.18437461825221 23.58449117777859 28-29 23.708807278359917 26.775766791286472 26.161017711166824 23.35440821918679 30-31 23.71698225916919 26.690105206684567 26.030529963222932 23.56238257092331 32-33 23.512975011747617 26.881540469548774 26.11121481915106 23.49426969955255 34-35 23.737184120393113 26.69980070119905 26.132198165731623 23.430817012676215 36-37 23.71346675790707 26.738317144725738 26.008665313713365 23.53955078365383 38-39 23.45700951535791 26.779579813179932 26.38309104395484 23.380319627507323 40-41 23.769932039704784 26.76622974453457 26.06928448192217 23.39455373383847 42-43 23.68716842799833 26.823788057042346 26.08859461605877 23.400448898900557 44-45 23.49699451009584 26.664743649390527 26.303842932911508 23.534418907602124 46-47 23.762417173832798 26.781090845159344 26.040893377221717 23.415598603786137 48-49 23.669575277348763 26.658208568949775 26.197627378933007 23.47458877476845 50-51 23.677172033343986 26.69643203818251 26.094304880634862 23.532091047838644 52-53 23.60916951629182 26.773420905954836 26.260413057559916 23.356996520193423 54-55 23.58208731945577 26.655696259340427 26.165393850089387 23.596822571114416 56-57 23.470324110045013 26.72045868673353 26.31324964078592 23.495967562435542 58-59 23.691099085650308 26.69955215525285 26.18098525844374 23.428363500653106 60-61 23.731557353768594 26.511072897842375 26.10766692970623 23.6497028186828 62-63 23.5944580676077 26.67209333625188 26.223607380763635 23.509841215376788 64-65 23.76490994780332 26.672114904869147 26.104012361411293 23.458962785916235 66-67 23.846473145607998 26.58743007612065 26.03532066109715 23.5307761171742 68-69 23.67651441580594 26.660753040797164 26.20119059107425 23.461541952322644 70-71 23.92624248573424 26.612698628924303 25.98841442241648 23.472644462924983 72-73 23.81125487957416 26.6574546757722 26.050684837505745 23.480605607147897 74-75 23.797877786311712 26.58585477928661 26.04496004610532 23.571307388296358 76-77 24.019944166672822 26.649981905866195 25.91399378152626 23.41608014593473 78-79 23.978735652848894 26.585549481569405 25.96430759832314 23.471407267258563 80-81 24.008024904928213 26.570553118636653 25.928409575398547 23.493012401036587 82-83 24.016957427797095 26.620959665979534 25.97327961892524 23.388803287298128 84-85 24.20358300736739 26.429470877534783 25.765957125898208 23.60098898919962 86-87 24.074674790008892 26.553923155150084 25.91510417998728 23.456297874853746 88-89 24.24345111683128 26.553129143873065 25.841082054021758 23.3623376852739 90-91 24.416863077049598 26.468110502986587 25.57757162992071 23.53745479004311 92-93 24.326721806806493 26.53695483628173 25.70423721589366 23.432086141018118 94-95 24.551393218460362 26.52568396357905 25.54347685624313 23.379445961717458 96-97 24.650834008333167 26.521525869172578 25.45441431443598 23.37322580805827 98-99 24.640845746600494 26.492360373047326 25.50010834132558 23.366685539026605 100-101 25.173084610750756 26.399221114646103 25.036208589476942 23.391485685126202 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 166.0 1 170.0 2 184.5 3 203.5 4 212.0 5 242.5 6 295.0 7 340.5 8 446.5 9 590.5 10 771.0 11 995.5 12 1326.0 13 1808.0 14 2396.0 15 3059.5 16 3813.0 17 4837.5 18 6193.5 19 7970.0 20 10086.5 21 12454.5 22 15427.5 23 18939.0 24 22705.0 25 26853.0 26 31325.5 27 35873.0 28 40389.0 29 45040.5 30 49758.0 31 54225.5 32 58675.5 33 62808.0 34 66538.0 35 70025.5 36 72807.5 37 75223.0 38 76565.0 39 76178.0 40 74947.0 41 73185.0 42 70737.0 43 67720.5 44 65058.0 45 63247.0 46 61384.0 47 59696.0 48 58521.0 49 56921.0 50 55073.0 51 53618.0 52 51919.5 53 50253.0 54 49392.5 55 49037.5 56 48740.0 57 48560.5 58 48543.5 59 48371.0 60 48196.5 61 47946.0 62 47710.0 63 47020.0 64 45789.5 65 44425.0 66 42586.0 67 40264.5 68 37295.5 69 33675.5 70 30027.0 71 26378.0 72 22636.0 73 19054.0 74 15535.0 75 12462.0 76 9959.0 77 7841.5 78 6108.5 79 4707.5 80 3708.5 81 3031.0 82 2444.5 83 1882.0 84 1504.5 85 1197.5 86 925.5 87 735.5 88 588.5 89 452.0 90 330.5 91 241.0 92 180.0 93 143.0 94 105.0 95 70.5 96 50.5 97 32.5 98 17.0 99 11.5 100 6.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.36196041595053 2 10.707141636045124 3 9.411900949803954 4 11.368787533983937 5 8.89437084449281 6 5.093280049705243 7 9.468517868336253 8 9.763183194333896 9 5.204602146663554 10-11 6.116722762758568 12-13 5.209822667723025 14-15 7.118658399616182 16-17 2.539084975009513 18-19 2.9568369524656855 20-21 2.9695757591354526 22-23 3.0079208539596 24-25 3.3602692612411236 26-27 4.146398851485367 28-29 1.6678645679191628 30-31 2.1441635861303254 32-33 2.907094945469452 34-35 1.8815199069129627 36-37 2.918785971504043 38-39 1.3179242178869022 40-41 1.1059416514861022 42-43 0.517658780125991 44-45 1.22369748918672 46-47 1.2994685730146855 48-49 1.2010691038902073 50-51 1.5268553529642082 52-53 2.37919732650499 54-55 1.6975884501486194 56-57 1.7219631365037582 58-59 1.4902381770822803 60-61 1.3012516383061248 62-63 0.5695147305089824 64-65 1.1880729475907479 66-67 0.5365004494427461 68-69 0.7580600984913797 70-71 0.9525428900731057 72-73 0.08089969430540417 74-75 0.10213103875501603 76-77 0.440251687937839 78-79 0.8225629449621052 80-81 0.2749780793261851 82-83 0.49170323175988545 84-85 0.06264625271365994 86-87 0.09553185896505009 88-89 0.07966809250616261 90-91 0.08450258912109587 92-93 0.15986926638811633 94-95 0.42624451523601714 96-97 0.3507491631541506 98-99 0.06521974901058258 100-101 0.2169089735977662 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2720035.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.63953999380308 #Duplication Level Percentage of deduplicated Percentage of total 1 98.91547626874335 97.56977077416789 2 0.9073624273585715 1.7900362488462014 3 0.10167249326479864 0.30086783896988345 4 0.04772909254732041 0.1883190293275736 5 0.01529576719540861 0.07543837200037044 6 0.01063767134056242 0.06295770045830038 7 0.001826279549990553 0.012610036229781048 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.4705693125272284E-4 3.676423281318071E-5 0.0 2 1.4705693125272284E-4 3.676423281318071E-5 0.0 3 2.205853968790843E-4 3.676423281318071E-5 3.676423281318071E-5 4 2.57349629692265E-4 3.676423281318071E-5 3.676423281318071E-5 5 3.308780953186264E-4 3.676423281318071E-5 3.676423281318071E-5 6 4.0440656094498784E-4 3.676423281318071E-5 3.676423281318071E-5 7 4.411707937581686E-4 3.676423281318071E-5 3.676423281318071E-5 8 5.1469925938453E-4 3.676423281318071E-5 3.676423281318071E-5 9 5.1469925938453E-4 3.676423281318071E-5 3.676423281318071E-5 10-11 5.1469925938453E-4 3.676423281318071E-5 3.676423281318071E-5 12-13 5.1469925938453E-4 9.191058203295179E-5 3.676423281318071E-5 14-15 5.330813757911204E-4 1.1029269843954214E-4 3.676423281318071E-5 16-17 5.514634921977107E-4 1.1029269843954214E-4 7.352846562636142E-5 18-19 6.249919578240721E-4 1.1029269843954214E-4 7.352846562636142E-5 20-21 7.352846562636143E-4 1.1029269843954214E-4 7.352846562636142E-5 22-23 8.088131218899757E-4 1.1029269843954214E-4 1.4705693125272284E-4 24-25 8.088131218899757E-4 1.1029269843954214E-4 1.4705693125272284E-4 26-27 8.27195238296566E-4 1.1029269843954214E-4 1.4705693125272284E-4 28-29 9.007237039229275E-4 2.2058539687908426E-4 2.205853968790843E-4 30-31 0.0010477806351756502 2.57349629692265E-4 2.205853968790843E-4 32-33 0.0013602766140876864 2.941138625054457E-4 2.3896751328567463E-4 34-35 0.001709536825812903 2.941138625054457E-4 2.57349629692265E-4 36-37 0.0020955612703513005 2.941138625054457E-4 2.57349629692265E-4 38-39 0.0029595207414610473 2.941138625054457E-4 2.57349629692265E-4 40-41 0.004044065609449879 3.308780953186264E-4 2.57349629692265E-4 42-43 0.005845513017295733 3.308780953186264E-4 2.57349629692265E-4 44-45 0.008602830478284286 3.308780953186264E-4 2.57349629692265E-4 46-47 0.012334400108822129 3.308780953186264E-4 2.57349629692265E-4 48-49 0.01759168540110697 3.308780953186264E-4 2.57349629692265E-4 50-51 0.023621019582468608 3.308780953186264E-4 2.57349629692265E-4 52-53 0.032076793129500174 3.676423281318071E-4 2.941138625054457E-4 54-55 0.04378620128049823 3.676423281318071E-4 2.941138625054457E-4 56-57 0.05795881302997939 3.676423281318071E-4 2.941138625054457E-4 58-59 0.07907986478115171 3.676423281318071E-4 2.941138625054457E-4 60-61 0.10398763251208165 3.676423281318071E-4 2.941138625054457E-4 62-63 0.1354945800329775 3.676423281318071E-4 3.1249597891203605E-4 64-65 0.17349041464539977 3.676423281318071E-4 4.411707937581685E-4 66-67 0.2165229491532278 3.676423281318071E-4 4.7793502657134926E-4 68-69 0.26847081011825213 3.676423281318071E-4 4.7793502657134926E-4 70-71 0.3297200219850112 3.676423281318071E-4 4.7793502657134926E-4 72-73 0.39769708845658236 3.676423281318071E-4 4.7793502657134926E-4 74-75 0.47615196127991 3.676423281318071E-4 4.7793502657134926E-4 76-77 0.5665184455347082 3.676423281318071E-4 4.7793502657134926E-4 78-79 0.6715722407983721 3.676423281318071E-4 4.7793502657134926E-4 80-81 0.7913684934201215 3.676423281318071E-4 4.7793502657134926E-4 82-83 0.9233337071030336 3.676423281318071E-4 4.7793502657134926E-4 84-85 1.074581760896459 3.8602444453839747E-4 5.330813757911204E-4 86-87 1.247079541255903 4.0440656094498784E-4 6.249919578240721E-4 88-89 1.4393197146360248 4.0440656094498784E-4 6.249919578240721E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCACG 945 0.0037068885 6.5449185 1 GGTTCAT 1125 4.8181746E-4 6.497319 1 TCGGAAG 6120 0.0 5.752815 94-95 GAGCGTC 4465 0.0 5.4941735 94-95 AGAGCGT 4440 0.0 5.473951 94-95 TAGATCG 2410 0.0 5.4664702 92-93 GGTTGCC 1340 0.003916086 5.4548388 1 ATCGGAA 6385 0.0 5.371754 94-95 GTCCTTT 1680 3.3418398E-4 5.3549333 1 GCGTCGT 4120 0.0 5.347793 94-95 AGCGTCG 4425 0.0 5.2871795 94-95 GATCGGA 7040 0.0 5.2591434 94-95 GTCGTGT 2205 0.0 5.253657 94-95 AAGAGCG 5105 0.0 5.2057843 92-93 CGGAAGA 6125 0.0 5.1918488 94-95 TCGTGTA 1865 0.0 5.1152453 92-93 >>END_MODULE