FastQCFastQC Report
Thu 11 Sep 2014
LONLib33_CAGGCG_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib33_CAGGCG_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720035
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCACG9450.00370688856.54491851
GGTTCAT11254.8181746E-46.4973191
TCGGAAG61200.05.75281594-95
GAGCGTC44650.05.494173594-95
AGAGCGT44400.05.47395194-95
TAGATCG24100.05.466470292-93
GGTTGCC13400.0039160865.45483881
ATCGGAA63850.05.37175494-95
GTCCTTT16803.3418398E-45.35493331
GCGTCGT41200.05.34779394-95
AGCGTCG44250.05.287179594-95
GATCGGA70400.05.259143494-95
GTCGTGT22050.05.25365794-95
AAGAGCG51050.05.205784392-93
CGGAAGA61250.05.191848894-95
TCGTGTA18650.05.115245392-93
Produced by FastQC (version 0.11.2)