##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	LONLib33_CAGGCG_L007_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2720035
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.199475374397757	33.0	33.0	33.0	27.0	33.0
2	31.60919399934192	33.0	33.0	33.0	27.0	33.0
3	31.773011376691844	33.0	33.0	33.0	27.0	33.0
4	35.598262889999575	37.0	37.0	37.0	33.0	37.0
5	35.12441935489801	37.0	37.0	37.0	33.0	37.0
6	34.92081940122094	37.0	33.0	37.0	33.0	37.0
7	34.71511432757299	37.0	33.0	37.0	27.0	37.0
8	34.75232708402649	37.0	33.0	37.0	27.0	37.0
9	34.659305854520255	37.0	33.0	37.0	27.0	37.0
10-11	34.50353727066012	37.0	33.0	37.0	27.0	37.0
12-13	34.51314027209209	37.0	33.0	37.0	27.0	37.0
14-15	35.412133851218826	37.0	33.0	40.0	27.0	40.0
16-17	35.308166990498286	37.0	33.0	40.0	27.0	40.0
18-19	35.51790638723399	37.0	33.0	40.0	27.0	40.0
20-21	35.17891681540863	37.0	33.0	40.0	27.0	40.0
22-23	35.20089649581715	37.0	33.0	40.0	27.0	40.0
24-25	35.022446953807574	37.0	33.0	40.0	27.0	40.0
26-27	34.610727803134886	37.0	33.0	40.0	27.0	40.0
28-29	34.22965844189505	37.0	33.0	40.0	24.5	40.0
30-31	34.09952776342951	37.0	33.0	38.5	22.0	40.0
32-33	34.079427838244726	37.0	33.0	37.0	24.5	40.0
34-35	33.90149207638872	37.0	33.0	37.0	22.0	40.0
36-37	33.56792908914775	37.0	33.0	37.0	22.0	40.0
38-39	33.441511414375185	37.0	33.0	37.0	22.0	40.0
40-41	33.12401274248309	37.0	33.0	37.0	22.0	40.0
42-43	32.71225701139875	37.0	33.0	37.0	22.0	40.0
44-45	32.55085486032349	37.0	33.0	37.0	22.0	40.0
46-47	32.37922453203727	37.0	30.0	37.0	22.0	40.0
48-49	32.385971320221984	37.0	33.0	37.0	22.0	40.0
50-51	32.685640625947826	37.0	33.0	37.0	22.0	40.0
52-53	32.541357739882024	37.0	33.0	37.0	22.0	40.0
54-55	32.16121704316305	37.0	30.0	37.0	18.5	40.0
56-57	32.18819059313575	37.0	27.0	37.0	22.0	40.0
58-59	31.890654348197724	33.0	27.0	37.0	22.0	40.0
60-61	31.784464905782464	33.0	27.0	37.0	22.0	40.0
62-63	31.765777830064685	33.0	27.0	37.0	22.0	40.0
64-65	31.418386895756857	33.0	27.0	37.0	15.0	40.0
66-67	31.070093583354627	33.0	27.0	37.0	15.0	40.0
68-69	30.887997764734646	33.0	27.0	37.0	15.0	38.5
70-71	30.73319938897845	33.0	27.0	37.0	15.0	37.0
72-73	30.514657348159123	33.0	27.0	37.0	15.0	37.0
74-75	30.170717472385466	33.0	27.0	37.0	15.0	37.0
76-77	29.098590643135108	33.0	27.0	35.0	15.0	37.0
78-79	30.03590156009022	33.0	27.0	37.0	15.0	37.0
80-81	29.770613061964276	33.0	27.0	37.0	15.0	37.0
82-83	29.753175602519818	33.0	27.0	37.0	15.0	37.0
84-85	29.628347245531767	33.0	27.0	37.0	15.0	37.0
86-87	29.424550787030313	33.0	27.0	37.0	15.0	37.0
88-89	29.291967198951482	33.0	27.0	37.0	15.0	37.0
90-91	29.195438661634867	33.0	27.0	33.0	15.0	37.0
92-93	28.9769653699309	33.0	27.0	33.0	15.0	37.0
94-95	28.8745503274774	33.0	27.0	33.0	15.0	37.0
96-97	28.731359522947315	33.0	27.0	33.0	15.0	37.0
98-99	28.47290990740928	33.0	27.0	33.0	15.0	37.0
100-101	27.654361432849207	33.0	24.5	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	1.0662821394541808
2306	2	0.4339143025053822
2306	3	0.4381994543362815
2306	4	0.28674878956673666
2306	5	0.6236342538430009
2306	6	0.7415619541274054
2306	7	0.9002817255246356
2306	8	0.8340058798041952
2306	9	1.0074067720524056
2306	10-11	1.228254510649677
2306	12-13	1.299395096721618
2306	14-15	1.4632185971194502
2306	16-17	1.4299675515632089
2306	18-19	1.2354346773683176
2306	20-21	1.467659422891039
2306	22-23	1.157525009711577
2306	24-25	1.0717230561333082
2306	26-27	1.1840697711168247
2306	28-29	1.5236804970959312
2306	30-31	1.4116419735486474
2306	32-33	1.1171708013566573
2306	34-35	0.9815314380420332
2306	36-37	1.1989393283786853
2306	38-39	1.0172429911433127
2306	40-41	1.2001369922288703
2306	42-43	0.9886180534951166
2306	44-45	0.8233413061517396
2306	46-47	0.8315267816115295
2306	48-49	0.7540186775242361
2306	50-51	0.9626210901672465
2306	52-53	0.9635761650986581
2306	54-55	1.4149623598339822
2306	56-57	1.1121200917237566
2306	58-59	0.9056212246344515
2306	60-61	0.7025427150633163
2306	62-63	0.5411883103483071
2306	64-65	0.5236447276283123
2306	66-67	0.7952389063377154
2306	68-69	0.6110309941267289
2306	70-71	0.4214032570526065
2306	72-73	0.361363842256349
2306	74-75	0.45739612591568246
2306	76-77	0.400038142660442
2306	78-79	0.44081901444494775
2306	80-81	1.0282455241346469
2306	82-83	0.8340626330195917
2306	84-85	0.5856825015381553
2306	86-87	0.38528691305286245
2306	88-89	0.28627610810560356
2306	90-91	-0.04101804123169117
2306	92-93	-0.10496750515310893
2306	94-95	-0.17411010995306597
2306	96-97	-0.15413310668409252
2306	98-99	-0.10788808391397353
2306	100-101	-0.220979248215464
2307	1	0.6106612533907132
2307	2	0.44980924236242004
2307	3	0.35526887121585915
2307	4	0.34990358423650036
2307	5	0.5617772317295078
2307	6	0.6155033667220167
2307	7	0.7584297910135334
2307	8	0.7838568249188995
2307	9	1.0107833371891246
2307	10-11	1.1816185353024125
2307	12-13	1.3218653452061133
2307	14-15	1.3619840295911914
2307	16-17	1.3425343002529644
2307	18-19	1.228904850653855
2307	20-21	1.4579293009176624
2307	22-23	1.1863583728260423
2307	24-25	1.2169278947496878
2307	26-27	1.3362105190712796
2307	28-29	1.5357759119972627
2307	30-31	1.6116540828333328
2307	32-33	1.3085882572599346
2307	34-35	1.272754900952492
2307	36-37	1.4207418606336262
2307	38-39	1.0422815258039648
2307	40-41	1.2572249226639585
2307	42-43	1.2611055087540706
2307	44-45	1.1370541743307072
2307	46-47	1.0235194491361597
2307	48-49	0.9477283696594725
2307	50-51	0.9147744610004551
2307	52-53	1.191775017075841
2307	54-55	1.6224037720715465
2307	56-57	1.3068041981406644
2307	58-59	1.2381483754963583
2307	60-61	1.0156490616083573
2307	62-63	0.8459706291337774
2307	64-65	0.8899528964820043
2307	66-67	1.0765759306637825
2307	68-69	0.9034901451343131
2307	70-71	0.7218733906238697
2307	72-73	0.7321374643287903
2307	74-75	0.9829188122444492
2307	76-77	0.9814249361740366
2307	78-79	0.9078574124401406
2307	80-81	1.542032833241759
2307	82-83	1.1809654053783873
2307	84-85	1.0980210359406364
2307	86-87	1.0454826423781327
2307	88-89	0.801289396459449
2307	90-91	0.6560035086809428
2307	92-93	0.751618698809434
2307	94-95	0.6442212185324152
2307	96-97	0.5899016218325563
2307	98-99	0.679051441455897
2307	100-101	0.6309461307963531
1101	1	1.0869869247292314
1101	2	0.6017057878633416
1101	3	0.40710024099228903
1101	4	0.360426817833563
1101	5	0.5955053245261013
1101	6	0.601201280235145
1101	7	0.7467943754695554
1101	8	0.7490895553141854
1101	9	0.9639512299060655
1101	10-11	1.0271599876905597
1101	12-13	1.1893512139291502
1101	14-15	1.2428233821911903
1101	16-17	1.3289562587582964
1101	18-19	0.9762600460863595
1101	20-21	1.3072478619495271
1101	22-23	1.0077194963883471
1101	24-25	1.18793963597345
1101	26-27	1.1824868898566976
1101	28-29	1.3281327691055012
1101	30-31	1.3824082129759745
1101	32-33	0.9717350634482855
1101	34-35	1.0553463149911408
1101	36-37	1.2267479779297332
1101	38-39	1.1015558187140257
1101	40-41	1.0861639093014261
1101	42-43	1.1109137809424325
1101	44-45	0.9818226303040873
1101	46-47	0.7606823535343423
1101	48-49	0.829042086863673
1101	50-51	0.7139917474469897
1101	52-53	1.1173322396992944
1101	54-55	1.4807291275889973
1101	56-57	1.166076510487425
1101	58-59	1.0793819198356474
1101	60-61	0.7710611688649749
1101	62-63	0.5940329194251142
1101	64-65	0.7486484682322221
1101	66-67	0.7584729322006609
1101	68-69	0.6968386483446984
1101	70-71	0.8436331756967377
1101	72-73	0.7313114512727665
1101	74-75	0.7474432127421444
1101	76-77	0.8551764603848895
1101	78-79	0.8633524811047799
1101	80-81	1.4567364142324344
1101	82-83	1.204210762033533
1101	84-85	1.1661810670309798
1101	86-87	0.8943960459893923
1101	88-89	0.7960435763412441
1101	90-91	0.5214336145879095
1101	92-93	0.6940799307597594
1101	94-95	0.6492925999750376
1101	96-97	0.6751939615566656
1101	98-99	0.733821908094896
1101	100-101	0.6534604373419235
2201	1	1.4380913407136084
2201	2	0.7541729950187381
2201	3	0.31122412398883625
2201	4	0.2107997157225796
2201	5	0.35808331928070203
2201	6	0.48884876288558843
2201	7	0.6158308816910463
2201	8	0.5989625637955669
2201	9	0.6538486013886597
2201	10-11	0.8182741223889565
2201	12-13	1.142464685158636
2201	14-15	1.0039459854537967
2201	16-17	0.9775230923095961
2201	18-19	0.7730399682542313
2201	20-21	1.1892757805598038
2201	22-23	0.8936275116787655
2201	24-25	0.8485609089874444
2201	26-27	1.0818658447627243
2201	28-29	1.3215080408581983
2201	30-31	1.4020930280153365
2201	32-33	1.046271359184793
2201	34-35	0.8980848302309283
2201	36-37	1.0465378891043002
2201	38-39	0.7008486613463631
2201	40-41	1.113765491930259
2201	42-43	1.0470132362562197
2201	44-45	0.9900853418252993
2201	46-47	0.7959812810058509
2201	48-49	0.864352467173795
2201	50-51	0.7740032150634661
2201	52-53	0.8975006009739985
2201	54-55	1.2926262567715803
2201	56-57	0.9443050649831797
2201	58-59	1.0582897839656482
2201	60-61	0.8281761056031058
2201	62-63	0.785065898115473
2201	64-65	0.8598883693081198
2201	66-67	0.7305045745465257
2201	68-69	0.8033737346841789
2201	70-71	0.8837914601785997
2201	72-73	0.7193954831653144
2201	74-75	0.6436673368934152
2201	76-77	0.7478123789365085
2201	78-79	0.7489861824379958
2201	80-81	1.3153584962203766
2201	82-83	1.1187033573755016
2201	84-85	0.9893573666006326
2201	86-87	1.0610144004336028
2201	88-89	0.9284095028902364
2201	90-91	0.8829839998452016
2201	92-93	0.9044803131879426
2201	94-95	0.7860838550535405
2201	96-97	0.48653538650521
2201	98-99	0.7829143161048009
2201	100-101	0.9533641283474097
2304	1	-1.9542157632930781
2304	2	-0.2977034051362253
2304	3	-0.15438647029213826
2304	4	-0.09380862664794876
2304	5	0.32204572474150694
2304	6	0.44145996077605076
2304	7	0.47617946504784214
2304	8	0.8090719311957812
2304	9	0.9676655021253282
2304	10-11	0.9247327551035767
2304	12-13	1.1520341457781669
2304	14-15	1.150258300806783
2304	16-17	1.2011191021772731
2304	18-19	0.8681438049348742
2304	20-21	1.2202908903839855
2304	22-23	0.9393663120464097
2304	24-25	1.09436786399813
2304	26-27	1.1471749482912443
2304	28-29	1.4776151985124173
2304	30-31	1.5207388308062306
2304	32-33	0.8530407977053684
2304	34-35	0.8914372387404299
2304	36-37	1.1235913425594646
2304	38-39	1.074721939837012
2304	40-41	1.2132469178182461
2304	42-43	0.9684505161170662
2304	44-45	1.0166084801640167
2304	46-47	1.1006788303393762
2304	48-49	0.9546611445722561
2304	50-51	0.9878772732973502
2304	52-53	1.1342276216117
2304	54-55	1.556011749420545
2304	56-57	1.0576807790687504
2304	58-59	0.928992243663096
2304	60-61	0.6043029243000149
2304	62-63	0.3854362163479017
2304	64-65	0.5248533690786026
2304	66-67	0.6858726469988063
2304	68-69	0.7164051880164379
2304	70-71	0.6539919685741822
2304	72-73	0.5272556090396456
2304	74-75	0.6887507193313844
2304	76-77	0.672774016017538
2304	78-79	0.7471044631333044
2304	80-81	1.2976875369537773
2304	82-83	0.939000724385167
2304	84-85	0.7250713191004401
2304	86-87	0.5770884746959588
2304	88-89	0.4559840610718666
2304	90-91	0.3276275749477833
2304	92-93	0.35436788587488977
2304	94-95	0.18629451282041742
2304	96-97	0.25604776962035203
2304	98-99	0.7541884217961226
2304	100-101	0.6598886579633714
1102	1	1.435019659656131
1102	2	0.11664024855514654
1102	3	0.25907921886396323
1102	4	0.18188229400379896
1102	5	0.28893085294004806
1102	6	0.21587099184394276
1102	7	-4.741264352489683
1102	8	-1.1108713767521579
1102	9	0.15305182511283277
1102	10-11	0.7180504469382782
1102	12-13	0.9451129727790075
1102	14-15	0.9511630040059273
1102	16-17	0.5941543056057697
1102	18-19	0.8919535963564016
1102	20-21	1.1572174330882632
1102	22-23	0.7036927512440059
1102	24-25	1.0064178575247382
1102	26-27	1.0266264203206035
1102	28-29	0.9488136264412432
1102	30-31	1.2418569751361943
1102	32-33	0.9646990157204627
1102	34-35	1.2443957992200154
1102	36-37	1.2286628405973303
1102	38-39	0.9217854024162406
1102	40-41	1.221005736084237
1102	42-43	1.1834981640104445
1102	44-45	1.151951881004301
1102	46-47	1.012317053512902
1102	48-49	1.0110375975786354
1102	50-51	0.81481038404511
1102	52-53	1.0078640258124096
1102	54-55	1.3582124834323572
1102	56-57	1.2355278805604009
1102	58-59	1.1593021417350329
1102	60-61	1.0691318627683017
1102	62-63	0.7782945000772408
1102	64-65	0.4688085718445514
1102	66-67	0.5142365382417431
1102	68-69	0.7295593599071708
1102	70-71	0.7460756507890522
1102	72-73	0.6141191671479014
1102	74-75	0.7102257791891482
1102	76-77	0.7257500360671436
1102	78-79	0.6881832735703739
1102	80-81	1.3960257763192416
1102	82-83	1.186096401814794
1102	84-85	0.7769985818218395
1102	86-87	0.5871567140648075
1102	88-89	0.5984631526874544
1102	90-91	0.24569004944717676
1102	92-93	0.2871845843902001
1102	94-95	0.2817455426984239
1102	96-97	0.19933087190030108
1102	98-99	0.24867830487954024
1102	100-101	0.4542864177298256
2202	1	1.5942293927160556
2202	2	0.9455519658567972
2202	3	0.417271204525246
2202	4	-0.2916779855761291
2202	5	0.14040923856129695
2202	6	0.45305710359624385
2202	7	0.7570267518782572
2202	8	-3.889437602736887
2202	9	-0.5432816070589084
2202	10-11	0.5285649482632095
2202	12-13	0.023627803095102706
2202	14-15	0.15661356687428452
2202	16-17	0.3121872393135874
2202	18-19	0.8414169755280057
2202	20-21	1.2327666969843492
2202	22-23	-2.6227235874358357
2202	24-25	-3.017064300454873
2202	26-27	-1.4008537070401843
2202	28-29	0.6093533081018094
2202	30-31	1.4537950394225092
2202	32-33	1.1747425978490682
2202	34-35	-2.031550365461591
2202	36-37	-0.3002000846290329
2202	38-39	0.2918981564080383
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2202	50-51	-3.9812572332060583
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2202	54-55	1.5308111262702333
2202	56-57	1.4050575032426842
2202	58-59	1.4616271229923754
2202	60-61	1.3969640318292775
2202	62-63	1.1427232555973426
2202	64-65	1.1652046098562785
2202	66-67	1.31996376280296
2202	68-69	1.0683676069132915
2202	70-71	1.2065169019012139
2202	72-73	1.0025007974444513
2202	74-75	0.5793531696995338
2202	76-77	1.1481441864276931
2202	78-79	1.1227250268816498
2202	80-81	1.778338780127207
2202	82-83	1.5696307715009468
2202	84-85	1.4740120992552868
2202	86-87	1.5813988167819915
2202	88-89	1.394058949144597
2202	90-91	1.280547150352426
2202	92-93	1.3447638562175825
2202	94-95	1.2380127874793452
2202	96-97	1.052998759925181
2202	98-99	1.311666495881724
2202	100-101	1.277716053043683
2305	1	1.259994218831629
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2305	3	0.11758424895741726
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2305	100-101	1.0599977099992728
1103	1	1.348908924075328
1103	2	0.7094546819147922
1103	3	0.1978956954143989
1103	4	-0.3168946550374869
1103	5	0.12601860211970006
1103	6	0.2347245252047543
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1103	9	0.08177748879878521
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2203	2	0.7055063282326834
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1209	82-83	-9.987236771937276
1209	84-85	-3.4791669066032007
1209	86-87	-2.327548765423682
1209	88-89	-1.986286796778483
1209	90-91	-6.0448854803626055
1209	92-93	-3.780124795796187
1209	94-95	-0.5604408934265166
1209	96-97	0.1486525185661165
1209	98-99	1.2238700683588348
1209	100-101	0.6780971383987904
1212	1	-28.578002171060067
1212	2	-16.23788901825806
1212	3	-5.5915027762893
1212	4	-1.4602101340443596
1212	5	-0.34438159490822784
1212	6	-2.7837649290933015
1212	7	-0.36243615572460897
1212	8	-1.1416856853781354
1212	9	0.1033130566224898
1212	10-11	0.7609134771217754
1212	12-13	0.17784737869677514
1212	14-15	-0.2710493469671391
1212	16-17	-0.06352922913647063
1212	18-19	-1.099825613177586
1212	20-21	-0.6033559499904939
1212	22-23	-0.5275292281643758
1212	24-25	0.21881941483944445
1212	26-27	-0.7005570019885781
1212	28-29	0.18614110333719935
1212	30-31	-0.6537722057688029
1212	32-33	0.12084206882414605
1212	34-35	-1.384406361535028
1212	36-37	-1.3286039479532867
1212	38-39	-0.15693495213258757
1212	40-41	-0.3796089900530717
1212	42-43	0.02824947441877157
1212	44-45	1.0116633765084728
1212	46-47	-2.0355781529282133
1212	48-49	-1.3585826719971479
1212	50-51	-0.24252537336473523
1212	52-53	-1.5189298977866734
1212	54-55	-0.3843538637600652
1212	56-57	-1.5634237502715074
1212	58-59	-0.7487745898934293
1212	60-61	-0.0844762393829761
1212	62-63	-4.734540190175007
1212	64-65	-4.257692600732323
1212	66-67	-1.6931308236962046
1212	68-69	-0.0868180130360372
1212	70-71	-1.124065020179117
1212	72-73	-1.3370072037808605
1212	74-75	0.032427035633855184
1212	76-77	1.1024099114088486
1212	78-79	-1.141920602670801
1212	80-81	0.4117388803174826
1212	82-83	0.8440733464496972
1212	84-85	-3.476647522277144
1212	86-87	-1.690306388126853
1212	88-89	-0.5918456280153812
1212	90-91	0.41277152111723225
1212	92-93	-2.1658478350118777
1212	94-95	-1.8786338349112874
1212	96-97	0.2723094892160134
1212	98-99	-0.20333658474265448
1212	100-101	0.08920863488418007
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	14.0
11	22.0
12	88.0
13	347.0
14	1029.0
15	2378.0
16	4391.0
17	7264.0
18	11148.0
19	16198.0
20	21990.0
21	29036.0
22	37858.0
23	47755.0
24	59234.0
25	71713.0
26	86442.0
27	103364.0
28	120713.0
29	141737.0
30	163241.0
31	187962.0
32	213565.0
33	239997.0
34	262932.0
35	281163.0
36	283483.0
37	241855.0
38	82904.0
39	204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.35414492315937	26.986560847030816	15.934715443182643	20.72457878662717
2	25.721628259239104	26.695796997014664	27.048302180914423	20.534272562831806
3	20.919829104637564	24.220568398769572	30.043966475332784	24.815636021260083
4	23.82281845638016	24.55486786015621	28.67944713946696	22.942866543996676
5	21.75122746582305	26.690685965437943	27.614754957197242	23.943331611541762
6	21.78935197525032	27.386081429099256	26.69215653475047	24.13241006089995
7	23.607122702465226	27.369905166661457	27.420639807943648	21.60233232292967
8	22.014569665463863	26.573334534298272	27.885670588797574	23.526425211440294
9	22.668237724882218	26.075767407404683	27.60225511804076	23.65373974967234
10-11	23.178065723419	26.58230500710469	26.66737744183439	23.572251827641924
12-13	23.25141338295344	26.485559912170352	26.571691604710594	23.691335100165613
14-15	23.147128621506706	26.689307306707448	26.468593235013522	23.69497083677232
16-17	23.260429591639785	26.754423213021937	26.474866958943394	23.510280236394888
18-19	23.530022269796877	26.710875028936748	26.177776354709177	23.581326346557198
20-21	23.191341874524017	26.686352691482405	26.442182994370107	23.680122439623467
22-23	23.23362502086371	26.539686636269987	26.58378534974195	23.642902993124352
24-25	23.35894574885985	26.441773727176304	26.54677237609075	23.652508147873096
26-27	23.355195797112906	26.57857343747415	26.484015830678647	23.582214934734296
28-29	23.48289635978949	26.545816506037607	26.547765010376708	23.4235221237962
30-31	23.55271163790172	26.490394425071734	26.326775206936677	23.63011873008987
32-33	23.329865240704624	26.528684373546664	26.45241697257572	23.689033413172993
34-35	23.579641438437374	26.44052374326066	26.42206809838844	23.55776671991353
36-37	23.603391132834687	26.460946274588377	26.37671941721338	23.558943175363552
38-39	23.370894124524135	26.430891514263603	26.645282137913668	23.552932223298598
40-41	23.574410716478468	26.42941281574472	26.395423820164577	23.600752647612243
42-43	23.367272847592034	26.52443810465674	26.520890356190268	23.587398691560953
44-45	23.349331901979202	26.316867246193524	26.670153141411785	23.663647710415493
46-47	23.50600825321226	26.45977496721735	26.51331567764668	23.520901101923712
48-49	23.570753159703152	26.28309057901482	26.565127494729847	23.581028766552183
50-51	23.39601269690011	26.46250010110168	26.516708982314192	23.62477821968402
52-53	23.446466172738745	26.498359854156156	26.623064246474996	23.432109726630106
54-55	23.34870603494961	26.3121729517168	26.703831251266095	23.63528976206749
56-57	23.386720529352356	26.441331130884784	26.58318759207251	23.58876074769035
58-59	23.374699331864942	26.352447727948903	26.756055543176892	23.516797397009263
60-61	23.587621043065027	26.30513642815835	26.49322445600244	23.614018072774183
62-63	23.403408559284163	26.383034887245632	26.61812632364163	23.59543022982858
64-65	23.495888674447464	26.33551673877793	26.61886373333103	23.549730853443574
66-67	23.5740701864425	26.33778406552823	26.54306531020869	23.545080437820573
68-69	23.451351266392308	26.363798969194402	26.629965717634562	23.554884046778728
70-71	23.66733910475659	26.369407853738647	26.40306652357596	23.560186517928802
72-73	23.469884631524437	26.41832905668094	26.58956678252919	23.52221952926543
74-75	23.402206189711425	26.266927879999287	26.72259839222495	23.60826753806434
76-77	23.479641102847694	26.53298782357942	26.546408359097445	23.44096271447544
78-79	23.667816532703537	26.398285752310546	26.42604282972191	23.507854885264006
80-81	23.447757054972225	26.332974925366432	26.5696235538625	23.649644465798843
82-83	23.53815771114948	26.477844624217184	26.4777894754451	23.50620818918823
84-85	23.489647271546755	26.243689591313835	26.55715573046324	23.709507406676174
86-87	23.416081221588694	26.43628293726019	26.548292049467104	23.599343791684007
88-89	23.544782320318486	26.402954113949644	26.43365413321529	23.61860943251658
90-91	23.68901361092669	26.34502795490076	26.21981971116058	23.74613872301197
92-93	23.456332340539042	26.405153828575965	26.46572892616254	23.672784904722448
94-95	23.672250822621514	26.41948861971299	26.19170270978825	23.71655784787725
96-97	23.670668890621464	26.438503562513066	26.13213681476752	23.75869073209795
98-99	23.544362440722463	26.398582210901644	26.226800295250168	23.830255053125725
100-101	23.730086126766363	26.318944800037652	26.03490737254533	23.916061700650655
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.5
2	1.5
3	4.5
4	6.5
5	9.0
6	16.0
7	32.5
8	53.0
9	83.5
10	127.5
11	191.5
12	314.0
13	470.5
14	671.0
15	977.5
16	1480.0
17	2173.5
18	3169.0
19	4564.0
20	6354.0
21	8679.0
22	11414.5
23	14619.0
24	18412.0
25	22589.5
26	27053.0
27	32109.0
28	37111.5
29	41987.0
30	47158.0
31	52045.5
32	56700.5
33	60964.0
34	65244.0
35	69156.0
36	72858.5
37	76022.0
38	77439.0
39	77499.5
40	76541.5
41	74740.5
42	71979.5
43	69071.5
44	66577.0
45	64297.5
46	62249.0
47	60941.0
48	59869.5
49	58495.0
50	56887.0
51	54943.5
52	53138.0
53	51920.5
54	50915.5
55	50144.0
56	50167.5
57	50455.5
58	50902.0
59	51414.5
60	51644.5
61	51868.0
62	52177.0
63	52156.0
64	51828.5
65	51008.5
66	49230.0
67	46711.5
68	43599.5
69	39576.0
70	34638.5
71	29675.5
72	24664.5
73	19419.0
74	14615.0
75	10602.5
76	7374.5
77	4868.5
78	3116.0
79	1988.5
80	1268.5
81	830.0
82	559.0
83	375.0
84	251.5
85	167.5
86	128.5
87	93.5
88	64.5
89	41.0
90	26.0
91	21.0
92	12.0
93	8.5
94	6.0
95	3.5
96	1.5
97	1.0
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.6646605650294941
2	0.0027573174609885538
3	2.941138625054457E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.18406013157918924
14-15	0.0
16-17	3.676423281318071E-4
18-19	0.10668980362385043
20-21	1.8382116406590355E-5
22-23	3.676423281318071E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0013602766140876862
42-43	0.0
44-45	0.0
46-47	0.022389417783227054
48-49	3.676423281318071E-5
50-51	3.676423281318071E-5
52-53	9.191058203295178E-5
54-55	0.0022793824344172044
56-57	0.0011764554500217827
58-59	0.0935833546259515
60-61	0.0011764554500217827
62-63	0.0010845448679888312
64-65	0.0017830652914392646
66-67	0.003970537143823517
68-69	0.004540382752427818
70-71	0.0030146670906808183
72-73	0.03705834667568616
74-75	0.043547233767212555
76-77	0.14882161442775552
78-79	2.941138625054457E-4
80-81	0.10759052732777336
82-83	0.004393325821175095
84-85	0.004558764868834409
86-87	0.006139626879801179
88-89	0.006139626879801179
90-91	0.020790173655853693
92-93	0.009944724975965383
94-95	0.013823351537755948
96-97	0.009393261483767672
98-99	0.01130500159005307
100-101	0.011874847198657371
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2720035.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.60025516673514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.38483636190438	96.02385133459349
2	1.2247283090897998	2.3906759095417702
3	0.17124340637982377	0.5014020047487516
4	0.10932826859188544	0.42681867646021493
5	0.04894803370990658	0.2388670289998418
6	0.02794998320379241	0.16367552955576603
7	0.023992319894781955	0.16391595806408585
8	0.006468954602058567	0.050509729585835335
9	0.0	0.0
>10	0.0023820870923347568	0.025366174640852557
>50	0.0	0.0
>100	1.2227553121772156E-4	0.01491765380937214
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	1.4705693125272284E-4	0.0	3.676423281318071E-5
2	1.8382116406590355E-4	0.0	1.1029269843954214E-4
3	2.57349629692265E-4	0.0	1.1029269843954214E-4
4	2.57349629692265E-4	0.0	1.1029269843954214E-4
5	3.676423281318071E-4	0.0	1.1029269843954214E-4
6	4.7793502657134926E-4	0.0	1.1029269843954214E-4
7	5.1469925938453E-4	0.0	1.1029269843954214E-4
8	5.882277250108914E-4	3.676423281318071E-5	1.1029269843954214E-4
9	5.882277250108914E-4	3.676423281318071E-5	1.1029269843954214E-4
10-11	6.249919578240721E-4	3.676423281318071E-5	1.1029269843954214E-4
12-13	6.617561906372528E-4	3.676423281318071E-5	1.1029269843954214E-4
14-15	7.169025398570238E-4	3.676423281318071E-5	1.1029269843954214E-4
16-17	7.536667726702046E-4	3.676423281318071E-5	1.1029269843954214E-4
18-19	8.823415875163371E-4	3.676423281318071E-5	1.1029269843954214E-4
20-21	9.926342859558793E-4	3.676423281318071E-5	1.1029269843954214E-4
22-23	0.0010661627515822407	3.676423281318071E-5	1.1029269843954214E-4
24-25	0.0010661627515822407	3.676423281318071E-5	1.1029269843954214E-4
26-27	0.0010661627515822407	3.676423281318071E-5	1.1029269843954214E-4
28-29	0.0011213091008020117	3.676423281318071E-5	1.286748148461325E-4
30-31	0.0013786587304942767	3.676423281318071E-5	1.4705693125272284E-4
32-33	0.0017463010586260838	3.676423281318071E-5	1.4705693125272284E-4
34-35	0.002169089735977662	3.676423281318071E-5	1.4705693125272284E-4
36-37	0.002720553228175373	3.676423281318071E-5	1.4705693125272284E-4
38-39	0.003676423281318071	3.676423281318071E-5	1.8382116406590355E-4
40-41	0.005036699895405758	3.676423281318071E-5	1.8382116406590355E-4
42-43	0.007279318097009781	3.676423281318071E-5	1.8382116406590355E-4
44-45	0.010551334817382865	3.676423281318071E-5	2.0220328047249392E-4
46-47	0.014650546776052514	3.676423281318071E-5	2.205853968790843E-4
48-49	0.020404149211315295	7.352846562636142E-5	2.57349629692265E-4
50-51	0.027168768048940545	7.352846562636142E-5	2.757317460988553E-4
52-53	0.03672746858036753	7.352846562636142E-5	3.492602117252168E-4
54-55	0.04889642964153035	7.352846562636142E-5	3.676423281318071E-4
56-57	0.064796960333231	7.352846562636142E-5	3.676423281318071E-4
58-59	0.08722314234927125	7.352846562636142E-5	3.676423281318071E-4
60-61	0.11305001590053068	7.352846562636142E-5	3.676423281318071E-4
62-63	0.1458620936862945	7.352846562636142E-5	3.676423281318071E-4
64-65	0.18488732681748582	7.352846562636142E-5	3.676423281318071E-4
66-67	0.2300521868284783	7.352846562636142E-5	3.676423281318071E-4
68-69	0.2842794302279199	1.1029269843954214E-4	4.0440656094498784E-4
70-71	0.34858007341817293	1.1029269843954214E-4	4.227886773515782E-4
72-73	0.42324823026174296	1.1029269843954214E-4	4.7793502657134926E-4
74-75	0.5089272748328606	1.1029269843954214E-4	5.514634921977107E-4
76-77	0.6108928745402173	1.1029269843954214E-4	5.514634921977107E-4
78-79	0.7302111921353953	1.1029269843954214E-4	5.514634921977107E-4
80-81	0.8639594711097467	1.1029269843954214E-4	5.514634921977107E-4
82-83	1.0109061096640302	1.1029269843954214E-4	5.514634921977107E-4
84-85	1.1758304580639587	1.1029269843954214E-4	6.066098414174817E-4
86-87	1.3662875661526415	1.1029269843954214E-4	6.249919578240721E-4
88-89	1.5783068967862546	1.4705693125272284E-4	6.249919578240721E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGC	525	0.0042838403	8.190326	1
GGTTACG	550	0.006507297	7.818038	1
CACACGT	3600	0.0	6.5918193	94-95
GCACACG	4055	0.0	6.3788652	94-95
AGCACAC	4940	0.0	6.052731	94-95
TCGGAAG	6720	0.0	5.967951	94-95
GAGCACA	5285	0.0	5.882123	94-95
CGTCTGA	2890	0.0	5.8299966	94-95
ACACGTC	3355	0.0	5.729283	94-95
AGAGCAC	5345	0.0	5.6830573	92-93
GTGCAAA	1350	1.5059234E-4	5.6624475	1
CGGAAGA	6685	0.0	5.5022216	94-95
ACGTCTG	3075	0.0	5.4792485	94-95
CACGTCT	3225	0.0	5.4452996	92-93
ATCGGAA	7105	0.0	5.2773123	92-93
GATCGGA	7645	0.0	5.2458634	94-95
GTTGTCA	1200	0.0072567365	5.1758313	1
CTGAACT	3315	0.0	5.0825615	94-95
>>END_MODULE