##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib32_CACTCA_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6371517 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.018497792597902 33.0 22.0 33.0 2.0 33.0 2 25.00752285523212 33.0 15.0 33.0 2.0 33.0 3 25.92579522270756 33.0 22.0 33.0 2.0 33.0 4 28.112543370754562 33.0 22.0 37.0 2.0 37.0 5 28.200079353158753 33.0 22.0 37.0 6.0 37.0 6 29.27610865669824 33.0 27.0 37.0 15.0 37.0 7 28.9380563529847 33.0 27.0 37.0 6.0 37.0 8 28.692329785826516 33.0 27.0 37.0 2.0 37.0 9 29.48296190687398 33.0 27.0 37.0 15.0 37.0 10-11 29.123677218470892 33.0 27.0 37.0 10.5 37.0 12-13 29.429739338371064 33.0 27.0 37.0 10.5 37.0 14-15 29.81302592459535 33.0 27.0 37.0 6.0 40.0 16-17 30.742377364762582 33.0 27.0 37.0 15.0 40.0 18-19 31.080650808904693 33.0 27.0 37.0 15.0 40.0 20-21 31.110281742950697 33.0 27.0 37.0 15.0 40.0 22-23 31.17301648885187 33.0 27.0 37.0 15.0 40.0 24-25 30.76952764938083 33.0 27.0 37.0 10.5 40.0 26-27 30.257977652731682 33.0 27.0 37.0 15.0 40.0 28-29 30.51205615554349 33.0 27.0 37.0 15.0 40.0 30-31 30.45511186739359 33.0 27.0 37.0 15.0 40.0 32-33 30.306854866745233 33.0 27.0 37.0 15.0 40.0 34-35 30.357794697871796 33.0 27.0 37.0 15.0 40.0 36-37 30.596138564803326 33.0 27.0 37.0 15.0 40.0 38-39 30.674901204846506 33.0 27.0 37.0 15.0 40.0 40-41 30.79280318957008 33.0 27.0 37.0 15.0 40.0 42-43 30.737837001768966 33.0 27.0 37.0 15.0 40.0 44-45 30.507465961402914 33.0 27.0 37.0 15.0 40.0 46-47 30.14458150233296 33.0 27.0 37.0 15.0 40.0 48-49 30.010331997858597 33.0 27.0 37.0 15.0 40.0 50-51 29.254740668509555 33.0 24.5 37.0 15.0 38.5 52-53 28.974541070831325 33.0 24.5 37.0 15.0 38.5 54-55 29.945347866136117 33.0 27.0 37.0 15.0 40.0 56-57 29.8834673908898 33.0 27.0 37.0 15.0 40.0 58-59 30.104078510659235 33.0 27.0 37.0 15.0 40.0 60-61 29.865589937215894 33.0 27.0 37.0 15.0 40.0 62-63 29.766116766226943 33.0 27.0 37.0 15.0 40.0 64-65 29.741659246926595 33.0 27.0 37.0 15.0 40.0 66-67 29.621852378326857 33.0 27.0 37.0 15.0 40.0 68-69 29.34177229692709 33.0 27.0 37.0 15.0 40.0 70-71 29.242452778514128 33.0 27.0 37.0 15.0 38.5 72-73 29.007099879039792 33.0 27.0 37.0 15.0 37.0 74-75 28.96666790655977 33.0 27.0 37.0 15.0 37.0 76-77 28.56295973156785 33.0 27.0 37.0 15.0 37.0 78-79 27.97925917799482 33.0 22.0 37.0 10.5 37.0 80-81 27.863675950327057 33.0 22.0 35.0 15.0 37.0 82-83 27.431607025454063 30.0 22.0 33.0 10.5 37.0 84-85 27.539669516694374 33.0 22.0 33.0 15.0 37.0 86-87 27.22297931560098 27.0 22.0 33.0 15.0 37.0 88-89 26.8530586201057 27.0 22.0 33.0 6.0 37.0 90-91 26.721208858110245 27.0 22.0 33.0 6.0 37.0 92-93 26.602015972020478 27.0 22.0 33.0 6.0 37.0 94-95 26.378097398154942 27.0 22.0 33.0 6.0 37.0 96-97 26.2308534215635 27.0 22.0 33.0 6.0 37.0 98-99 25.987945492415697 27.0 22.0 33.0 6.0 37.0 100-101 25.4767202222014 27.0 22.0 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.343758253699171 2306 2 8.537904892045141 2306 3 7.503186704144461 2306 4 9.086254991738755 2306 5 8.857730308409053 2306 6 7.898483351457234 2306 7 8.172875491469433 2306 8 8.640715238331296 2306 9 7.48560509867697 2306 10-11 7.771297001215945 2306 12-13 7.39947739984499 2306 14-15 8.071464154301484 2306 16-17 6.856381547076921 2306 18-19 6.227818200189539 2306 20-21 6.173734180925315 2306 22-23 5.770155514098107 2306 24-25 5.925017781266128 2306 26-27 6.495836644732428 2306 28-29 5.598128772365936 2306 30-31 5.465949447534577 2306 32-33 5.563897848487457 2306 34-35 4.912029087409085 2306 36-37 4.897474895024207 2306 38-39 4.613817629749981 2306 40-41 3.8627447336606338 2306 42-43 3.5938775274651427 2306 44-45 3.428899930189665 2306 46-47 3.737160937152254 2306 48-49 3.537717951289661 2306 50-51 3.390641038959373 2306 52-53 3.9714303698398474 2306 54-55 3.517234034136191 2306 56-57 3.755104127941504 2306 58-59 3.2917584574643364 2306 60-61 3.354533194980487 2306 62-63 2.919895230270942 2306 64-65 2.5409649359515463 2306 66-67 2.3914990952887187 2306 68-69 2.6292849198511092 2306 70-71 2.670160865168718 2306 72-73 1.844682262779834 2306 74-75 1.4439286879273858 2306 76-77 1.8687612307930763 2306 78-79 2.117136358351008 2306 80-81 1.85264316730661 2306 82-83 1.823714909303927 2306 84-85 1.4964013295963419 2306 86-87 1.429702579125621 2306 88-89 1.4389141451682086 2306 90-91 1.2790648955031045 2306 92-93 1.2014365484688803 2306 94-95 1.3968777989723904 2306 96-97 1.1163910870458835 2306 98-99 1.0457304882353995 2306 100-101 0.853391476983532 2307 1 8.262810254113234 2307 2 8.360475450536217 2307 3 7.560144119128594 2307 4 9.08848232131718 2307 5 8.982767507540537 2307 6 7.567836927536636 2307 7 8.111549241834524 2307 8 8.57757537279172 2307 9 7.55350104690617 2307 10-11 7.6950962090138155 2307 12-13 7.2724882419642185 2307 14-15 8.11357280504006 2307 16-17 6.7866973184502655 2307 18-19 6.410737497835157 2307 20-21 6.44686961473429 2307 22-23 5.987590558215146 2307 24-25 6.124361309097559 2307 26-27 6.8974349392523635 2307 28-29 6.000637466253661 2307 30-31 5.947163935037203 2307 32-33 5.943037180280754 2307 34-35 5.750641675588657 2307 36-37 5.655638364367615 2307 38-39 5.23277291894216 2307 40-41 4.584237949558343 2307 42-43 4.409121595712765 2307 44-45 4.4074014673480875 2307 46-47 4.736511561968349 2307 48-49 4.490624396883927 2307 50-51 4.292710613033151 2307 52-53 4.930446481418571 2307 54-55 4.462503272283858 2307 56-57 4.831905729719317 2307 58-59 4.385541983001602 2307 60-61 4.487981442922855 2307 62-63 4.097952650603624 2307 64-65 4.011712272995769 2307 66-67 3.7077236661384774 2307 68-69 3.781040171722996 2307 70-71 3.4517165083429795 2307 72-73 2.9427714444823962 2307 74-75 2.562089236542665 2307 76-77 2.7890837093656913 2307 78-79 3.2147707614509144 2307 80-81 2.9536915232109493 2307 82-83 3.033819507730545 2307 84-85 2.3092741535653225 2307 86-87 2.303567436476662 2307 88-89 2.4471782529272765 2307 90-91 2.2436583883494734 2307 92-93 2.17299157365888 2307 94-95 2.335194347482396 2307 96-97 2.148650851398177 2307 98-99 1.9508892691136133 2307 100-101 1.640647044215381 1101 1 8.100581411565859 1101 2 7.934046858559292 1101 3 7.324047438496475 1101 4 8.749241956530405 1101 5 8.635636989532344 1101 6 7.390722569495463 1101 7 7.235977624920693 1101 8 8.168538526262644 1101 9 7.453328046356319 1101 10-11 7.555905788620091 1101 12-13 7.27648555509127 1101 14-15 7.768612046672036 1101 16-17 6.928298395442887 1101 18-19 6.022735451274386 1101 20-21 5.913803645451303 1101 22-23 5.446665429708972 1101 24-25 6.021551738971731 1101 26-27 6.5920261621558325 1101 28-29 6.042284381287185 1101 30-31 5.842706151819222 1101 32-33 5.909491812083644 1101 34-35 5.635106758533457 1101 36-37 5.458653324559645 1101 38-39 5.162845179401131 1101 40-41 4.596852786165989 1101 42-43 4.361994799639337 1101 44-45 4.3348529138131155 1101 46-47 4.650263954365123 1101 48-49 4.431630865345472 1101 50-51 4.012223175978939 1101 52-53 4.9421168044846695 1101 54-55 4.587573751341548 1101 56-57 4.772878120546672 1101 58-59 4.364320967374937 1101 60-61 4.5086304924796075 1101 62-63 4.177056784613363 1101 64-65 3.947593381755322 1101 66-67 3.6240089294445106 1101 68-69 3.735000593778338 1101 70-71 3.723441189577578 1101 72-73 3.1332684147008365 1101 74-75 2.5942779238444835 1101 76-77 2.9218512009421254 1101 78-79 3.4340723732765497 1101 80-81 2.9855740234397885 1101 82-83 3.085683236000609 1101 84-85 2.441444487287246 1101 86-87 2.5730165381947074 1101 88-89 2.564882171850229 1101 90-91 2.397916554676172 1101 92-93 2.2424824297435357 1101 94-95 2.4296063417210902 1101 96-97 2.3362381857067405 1101 98-99 2.2510779013469495 1101 100-101 1.9221481622864651 2201 1 7.201340188285311 2201 2 7.946698789088142 2201 3 6.3971134326387755 2201 4 8.457887265551832 2201 5 4.043402419474237 2201 6 5.896238479617299 2201 7 6.812924504597888 2201 8 7.835342491911312 2201 9 6.633058167087093 2201 10-11 4.233740564920417 2201 12-13 6.5613187878332795 2201 14-15 7.19319837215259 2201 16-17 6.076605296633851 2201 18-19 2.172709894213181 2201 20-21 5.194628853214688 2201 22-23 5.236582804643163 2201 24-25 5.585578709207276 2201 26-27 6.2692419008887725 2201 28-29 5.091831154963298 2201 30-31 5.260031997708374 2201 32-33 5.467785408843383 2201 34-35 5.107495382264762 2201 36-37 5.106459686625701 2201 38-39 4.542914222660677 2201 40-41 4.317542021744906 2201 42-43 4.222544393650853 2201 44-45 4.05148670124877 2201 46-47 4.46220609357907 2201 48-49 4.235613513883049 2201 50-51 4.000869315244579 2201 52-53 4.8259976273972 2201 54-55 4.398632516904595 2201 56-57 4.573074339871852 2201 58-59 4.208437445991677 2201 60-61 4.1372847142346565 2201 62-63 3.9310872211517136 2201 64-65 3.7166178418685227 2201 66-67 3.4058010884815957 2201 68-69 3.6303320986824517 2201 70-71 3.445366245996759 2201 72-73 2.7848960106267526 2201 74-75 2.4813703502342612 2201 76-77 2.8305013045575222 2201 78-79 3.142949221854053 2201 80-81 2.6465865986468557 2201 82-83 3.0042693182607643 2201 84-85 2.498936595223487 2201 86-87 2.4277839580950165 2201 88-89 2.5162147389889675 2201 90-91 2.324401601879906 2201 92-93 2.2076273746009747 2201 94-95 2.438812919178215 2201 96-97 2.2964437693989375 2201 98-99 2.134533591977064 2201 100-101 1.7407538245464913 2304 1 1.4118185939665118 2304 2 4.7638826298222625 2304 3 5.835105485752756 2304 4 4.491838952618991 2304 5 6.535607210487484 2304 6 6.388790277423215 2304 7 7.141672504466072 2304 8 6.619158931795894 2304 9 5.773419029493638 2304 10-11 6.7015092738388695 2304 12-13 6.548359172833202 2304 14-15 6.816615132764571 2304 16-17 5.4835998698830934 2304 18-19 5.278501223602973 2304 20-21 5.022337859662521 2304 22-23 4.636862652268604 2304 24-25 4.880175701022402 2304 26-27 5.787377059721081 2304 28-29 5.075127070031353 2304 30-31 5.049086932043203 2304 32-33 5.2010983911038835 2304 34-35 4.82460075884099 2304 36-37 4.891010877765279 2304 38-39 4.436857393162235 2304 40-41 3.8804282786397266 2304 42-43 3.460474242150813 2304 44-45 3.328148711972794 2304 46-47 4.25430325105097 2304 48-49 3.9690493047019366 2304 50-51 3.6352629710259237 2304 52-53 4.138681360921979 2304 54-55 3.9630138326930826 2304 56-57 4.559641430258353 2304 58-59 3.8623200907016653 2304 60-61 3.907581158464044 2304 62-63 3.6300567284156493 2304 64-65 3.5674544352080098 2304 66-67 3.3578960756460425 2304 68-69 3.5426566406824875 2304 70-71 3.5324297195071708 2304 72-73 3.070393320822596 2304 74-75 2.5340340850007053 2304 76-77 2.7173507501459433 2304 78-79 3.134941756966022 2304 80-81 2.9316984734403846 2304 82-83 3.080042804781108 2304 84-85 2.607116213595841 2304 86-87 2.4286435448660555 2304 88-89 2.6660191344403863 2304 90-91 2.637295752416538 2304 92-93 2.4121986614585076 2304 94-95 2.6869991316408672 2304 96-97 2.6143973838729693 2304 98-99 2.4194912090237857 2304 100-101 2.0788665464202545 1102 1 7.123706234798679 1102 2 7.517292545838828 1102 3 6.1957901812385145 1102 4 6.326524391385227 1102 5 6.578349777190304 1102 6 5.995156946264192 1102 7 6.844325547696798 1102 8 6.515282499722929 1102 9 5.032237795923791 1102 10-11 6.122263432797471 1102 12-13 5.945831864729307 1102 14-15 6.344975882627441 1102 16-17 5.381396656938719 1102 18-19 5.409792794892731 1102 20-21 5.64583566050468 1102 22-23 5.308733892732505 1102 24-25 1.9308003389357857 1102 26-27 5.596075916851063 1102 28-29 5.023358538679194 1102 30-31 4.914221798289212 1102 32-33 5.055533351378003 1102 34-35 4.985685843291922 1102 36-37 4.955171635668837 1102 38-39 4.008873848679855 1102 40-41 4.057608841920882 1102 42-43 4.142667735214136 1102 44-45 -3.649444320608847 1102 46-47 1.6318250529086775 1102 48-49 -0.4951973324907186 1102 50-51 2.897088960824604 1102 52-53 -0.15601459561445807 1102 54-55 2.818507965716808 1102 56-57 -0.5603194314634834 1102 58-59 2.9952203934476955 1102 60-61 3.9576842564791583 1102 62-63 3.6759976952000954 1102 64-65 3.3101110531057323 1102 66-67 3.283563034318515 1102 68-69 3.5180673614625633 1102 70-71 3.3985256521223803 1102 72-73 2.879441190734685 1102 74-75 2.6023547573470616 1102 76-77 2.8621917189811086 1102 78-79 -1.2527607204411808 1102 80-81 1.8148724085258081 1102 82-83 2.8002757173534256 1102 84-85 2.463154571904532 1102 86-87 2.430524365792813 1102 88-89 2.379912126382205 1102 90-91 2.192126007678116 1102 92-93 2.2107846056871345 1102 94-95 2.5312592093651567 1102 96-97 2.476829356195097 1102 98-99 2.384739421101333 1102 100-101 2.092397541536716 2202 1 -20.857013665904674 2202 2 -7.8546920415456345 2202 3 -0.7315971738075255 2202 4 1.6931644375706334 2202 5 1.2287314070464461 2202 6 1.8305548674920793 2202 7 4.1792236546854085 2202 8 2.8797988136036317 2202 9 3.392535807896575 2202 10-11 2.281905518970323 2202 12-13 1.5950417921515303 2202 14-15 2.864793966569515 2202 16-17 1.872777343067746 2202 18-19 1.8332786677611566 2202 20-21 1.1731786654881446 2202 22-23 1.4218389033578873 2202 24-25 2.484332097092974 2202 26-27 3.570611890201782 2202 28-29 -0.7765318435327622 2202 30-31 1.0238431275774218 2202 32-33 1.6032829917706444 2202 34-35 1.7173462143219602 2202 36-37 3.1347221013238773 2202 38-39 2.5160311234505492 2202 40-41 1.9375162930117291 2202 42-43 0.8598194520043201 2202 44-45 0.8274344021291391 2202 46-47 -1.231670393274058 2202 48-49 0.8625784273596686 2202 50-51 1.7566437031811653 2202 52-53 1.748492253786445 2202 54-55 -0.7150834098409042 2202 56-57 1.1717390223971975 2202 58-59 1.9364392088566156 2202 60-61 2.1388552226311326 2202 62-63 -2.0540954176697 2202 64-65 0.08215330216756911 2202 66-67 0.6605909675198482 2202 68-69 1.5736302518960628 2202 70-71 -2.469355025019553 2202 72-73 -4.550269418304879 2202 74-75 -1.0360748315899464 2202 76-77 0.05182431264103826 2202 78-79 1.704349161847702 2202 80-81 1.6628591308805625 2202 82-83 -0.6755285366907238 2202 84-85 -0.1912637257819263 2202 86-87 0.6563681589195163 2202 88-89 1.2526194985845187 2202 90-91 1.3900574429266115 2202 92-93 1.3379855197980781 2202 94-95 1.6584744286180495 2202 96-97 1.254840295877198 2202 98-99 1.248650355391181 2202 100-101 0.929719042635039 2305 1 5.811828400103156 2305 2 7.124398429476937 2305 3 6.782798753975708 2305 4 7.782598284039587 2305 5 7.835497022860096 2305 6 6.248143047340914 2305 7 3.069629251779947 2305 8 6.08566721560554 2305 9 6.446335193691532 2305 10-11 4.846226973109655 2305 12-13 5.2722446598884645 2305 14-15 5.672521900250292 2305 16-17 5.082221999771448 2305 18-19 4.480227918220088 2305 20-21 5.216709368717474 2305 22-23 5.09061674039981 2305 24-25 5.350867566605778 2305 26-27 6.145350922172241 2305 28-29 5.150361881663283 2305 30-31 5.38335255762329 2305 32-33 5.354432684686699 2305 34-35 5.2079460069610555 2305 36-37 4.669117595092462 2305 38-39 4.545520774616477 2305 40-41 4.031309162641534 2305 42-43 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1209 40-41 -23.575217217001573 1209 42-43 -24.174816507890707 1209 44-45 -27.035708284200076 1209 46-47 -20.586515677523487 1209 48-49 -20.319018990766246 1209 50-51 -22.570452775469718 1209 52-53 -25.210007148365804 1209 54-55 -22.350071737575348 1209 56-57 -26.118366597371633 1209 58-59 -26.425507781976386 1209 60-61 -26.168587981832797 1209 62-63 -26.320237306868584 1209 64-65 -26.208051958892614 1209 66-67 -13.906148640022867 1209 68-69 -25.899516420084808 1209 70-71 -25.748350640802734 1209 72-73 -15.887884005789932 1209 74-75 -16.922896170872423 1209 76-77 -14.059383003359024 1209 78-79 -15.133639533394879 1209 80-81 -24.78976143508197 1209 82-83 -16.437538547405396 1209 84-85 -13.705409683451201 1209 86-87 -14.02254735561776 1209 88-89 -11.50354985020141 1209 90-91 -13.975269846364409 1209 92-93 -18.08211471675294 1209 94-95 -23.46104530035764 1209 96-97 -19.18097803087377 1209 98-99 -10.556272700827213 1209 100-101 -5.71152287606802 1212 1 -20.857013665904674 1212 2 -20.629004516635312 1212 3 -21.552741055832236 1212 4 -23.609346522971414 1212 5 -23.80145990423295 1212 6 -24.886762788944747 1212 7 -24.453398904102954 1212 8 -24.091006288030485 1212 9 -25.018982725383616 1212 10-11 -24.74141054320584 1212 12-13 -25.03658435372332 1212 14-15 -25.41753963247393 1212 16-17 -26.49171050069471 1212 18-19 -21.036416019223754 1212 20-21 -26.913907823152748 1212 22-23 -27.13495663271226 1212 24-25 -23.215799481383556 1212 26-27 -25.895111505573425 1212 28-29 -20.745751971295135 1212 30-31 -15.525760279635024 1212 32-33 -26.132468303566142 1212 34-35 -22.317265055137913 1212 36-37 -26.572631425977836 1212 38-39 -26.869032646975917 1212 40-41 -21.33707695837179 1212 42-43 -17.592050396276797 1212 44-45 -14.69988100758614 1212 46-47 -17.981172485016977 1212 48-49 -20.83214772821093 1212 50-51 -12.947418317409607 1212 52-53 -5.454769867476536 1212 54-55 -4.1891788040872875 1212 56-57 -4.268003194207889 1212 58-59 -3.0368801633359404 1212 60-61 -14.529853479908898 1212 62-63 -12.678296306441052 1212 64-65 -16.922887358636096 1212 66-67 -13.090100323226366 1212 68-69 -8.553855881393059 1212 70-71 -15.90568283404771 1212 72-73 -12.463311538923747 1212 74-75 -8.522013481522439 1212 76-77 -19.805079354891202 1212 78-79 -10.32407542285813 1212 80-81 -8.091172294423568 1212 82-83 -5.572874299724905 1212 84-85 -4.814104291536577 1212 86-87 -3.811929126049126 1212 88-89 -3.6153365777961746 1212 90-91 -3.8161113370669497 1212 92-93 -3.7643893040724166 1212 94-95 -3.731460007497443 1212 96-97 -13.72362348930589 1212 98-99 -10.968007236065382 1212 100-101 -7.044565218505401 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1001.0 3 2413.0 4 1023.0 5 6470.0 6 8641.0 7 6421.0 8 12902.0 9 25254.0 10 32468.0 11 38145.0 12 52613.0 13 69559.0 14 81270.0 15 86601.0 16 90427.0 17 98770.0 18 113744.0 19 128326.0 20 139611.0 21 159574.0 22 182083.0 23 201775.0 24 213347.0 25 222649.0 26 238383.0 27 251811.0 28 258121.0 29 260888.0 30 271452.0 31 289356.0 32 320407.0 33 376682.0 34 439527.0 35 492353.0 36 526243.0 37 492560.0 38 178184.0 39 463.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.388580778162286 24.267503028928942 15.061329764153363 21.282586428755412 2 24.18141258251236 30.027625454387003 26.377685803145077 19.41327615995556 3 18.057037705009837 27.618066179201595 31.416200999877358 22.90869511591121 4 20.890660414391498 28.14067547818175 29.26835616433472 21.70030794309203 5 20.016826453291344 29.378100384901657 28.76836668362248 21.836706478184517 6 20.049549043549582 29.715484333675203 28.638576135500298 21.596390487274913 7 21.41161986291516 29.261117761999717 29.541077060305298 19.786185314779825 8 19.97191103748722 28.80961590612393 30.233719648539353 20.984753407849492 9 20.56478481040879 28.364869894320588 30.001379244483477 21.068966050787143 10-11 21.378330361665622 28.845577634427094 28.572996542832996 21.20309546107429 12-13 21.04746262029505 29.050008109405184 28.594045170716274 21.308484099583495 14-15 21.074201918331713 29.254630812280986 28.569425020899324 21.10174224848798 16-17 21.44598184895794 29.17223651962416 28.324133844989046 21.057647786428856 18-19 21.501646914052667 29.183531135236418 28.153401825859977 21.161420124850935 20-21 21.4284845837045 29.27074628341602 28.201222798885006 21.099546333994475 22-23 21.25333816801469 29.0795542925419 28.4989465756899 21.168160963753515 24-25 21.203301881991308 29.040699461561164 28.662900648365852 21.09309800808167 26-27 21.066310833036642 29.19275669481053 28.65123142960369 21.08970104254914 28-29 21.292055261699602 29.144469654928013 28.57652660265361 20.986948480718777 30-31 21.359785513998077 28.955666512293703 28.433466418737748 21.251081554970476 32-33 21.034030940519237 29.239758309508602 28.609558408670487 21.116652341301673 34-35 21.288464619751633 29.03982456617054 28.59647085217645 21.07523996190138 36-37 21.295274410826973 29.08470524217109 28.507447254107614 21.112573092894323 38-39 21.08938426297239 29.12715758720404 28.792300451170316 20.991157698653257 40-41 21.39517121614263 29.035760285327406 28.45911190382845 21.109956594701515 42-43 21.17923480318397 29.20849369807507 28.522316102928613 21.089955395812343 44-45 21.034761630420274 28.992556613759646 28.82681744682767 21.14586430899241 46-47 21.327920314400263 29.098041669482793 28.498227967896046 21.075810048220895 48-49 21.203093453481614 28.980410163786225 28.70209700697051 21.11439937576165 50-51 21.277171862764757 29.028848318025545 28.561120530429662 21.132859288780036 52-53 21.15167119704476 29.120131359896828 28.721861730568737 21.006335712489683 54-55 21.09227882485787 28.966508681327074 28.776526277179272 21.16468621663578 56-57 21.07408799566827 29.099266960072484 28.798312549910094 21.028332494349154 58-59 21.224037099429534 29.0575187921947 28.672892977015458 21.045551131360313 60-61 21.299681169825035 28.840260515099768 28.577142589988025 21.282915725087168 62-63 21.115144186594538 29.09971757415603 28.71318357648269 21.071954662766736 64-65 21.292930590421854 28.959084456875576 28.661324366298008 21.086660586404555 66-67 21.38240237938592 28.965400473524948 28.553578215484244 21.09861893160489 68-69 21.20110782911754 29.028587318460197 28.76516176706009 21.005143085362178 70-71 21.48497531353621 28.955308059581853 28.4708413128144 21.088875314067536 72-73 21.324572145574177 29.042830053294093 28.60247991575487 21.03011788537686 74-75 21.288398779968368 28.96656790923387 28.69222104926931 21.05281226152845 76-77 21.55372881163487 29.09422262228569 28.415180936905475 20.936867629173964 78-79 21.518817181942268 28.97310611254528 28.527281442259962 20.980795263252496 80-81 21.611226059737916 28.94676936635101 28.406602653321816 21.03540192058926 82-83 21.60274024248445 29.028923256899468 28.4479594175565 20.920377083059577 84-85 21.732999608626795 28.842289162605827 28.318984553008452 21.10572667575892 86-87 21.677473855406422 29.022005126210686 28.38054930688381 20.91997171149908 88-89 21.888909134319356 28.921315610902376 28.32583816082132 20.86393709395695 90-91 22.09848102240176 28.866465225474585 28.015738804029976 21.01931494809368 92-93 22.057582370587458 28.99084631494211 28.10866817184149 20.84290314262894 94-95 22.363761054781 28.888800055885945 27.906727554594056 20.840711334738998 96-97 22.489402983463794 28.980338041026464 27.750613818303165 20.779645157206573 98-99 22.565829232224726 28.99204759756911 27.763222698678135 20.678900471528024 100-101 23.104361077406562 28.85177691789887 27.302209366513395 20.741652638181172 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 443.0 1 458.5 2 522.5 3 558.5 4 607.0 5 750.0 6 910.0 7 1110.0 8 1435.5 9 1973.5 10 2725.5 11 3719.5 12 5122.0 13 6840.5 14 8894.0 15 11284.0 16 14101.0 17 17707.0 18 22147.5 19 27663.0 20 34539.5 21 42840.5 22 52283.0 23 63140.5 24 75904.0 25 89476.5 26 103525.0 27 118411.5 28 133725.0 29 148052.5 30 161547.0 31 174569.0 32 186157.5 33 197088.0 34 207118.5 35 215169.5 36 220854.5 37 224407.5 38 224872.0 39 222127.0 40 216469.0 41 207766.5 42 197370.0 43 186149.0 44 174793.0 45 164215.0 46 154058.5 47 144396.5 48 135451.5 49 126437.0 50 117700.0 51 109455.5 52 101907.0 53 95066.0 54 89508.0 55 85203.0 56 81324.0 57 77817.5 58 74942.5 59 71948.5 60 68911.5 61 66149.5 62 62864.0 63 59638.0 64 56524.5 65 53181.5 66 49845.0 67 46159.5 68 42063.5 69 37950.5 70 33699.0 71 29349.5 72 25014.5 73 20919.0 74 17198.5 75 13928.0 76 11053.0 77 8631.5 78 6790.5 79 5335.5 80 4238.0 81 3347.0 82 2620.5 83 2114.0 84 1686.0 85 1346.0 86 1065.5 87 845.0 88 668.5 89 501.5 90 376.5 91 282.0 92 209.5 93 154.5 94 113.0 95 82.0 96 60.0 97 34.5 98 18.5 99 7.0 100 2.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 14.0234735307149 2 11.394444996379983 3 10.035506457881224 4 11.960087370087846 5 9.411463549418452 6 5.550342249734247 7 9.999376914477352 8 10.409216517824563 9 5.66552674975206 10-11 6.552740893573697 12-13 5.553041763837403 14-15 7.5644858202528535 16-17 2.7675591228902 18-19 3.2457811852342227 20-21 3.184202443468329 22-23 3.2168320354477595 24-25 3.6267579604668714 26-27 4.516585296719761 28-29 1.7923439582755565 30-31 2.3475178674089703 32-33 3.1673461751730394 34-35 2.0951917730110425 36-37 3.2329741880936673 38-39 1.4224163570465245 40-41 1.199604427014791 42-43 0.5704999296086003 44-45 1.3550933003867054 46-47 1.4240486213879677 48-49 1.3211139513556975 50-51 1.608502339395783 52-53 2.5331879362481495 54-55 1.9063670394350356 56-57 1.8975465340514668 58-59 1.6158946134805885 60-61 1.4709762212044635 62-63 0.629685206835358 64-65 1.2747042815706213 66-67 0.5824280151806862 68-69 0.8323527976147596 70-71 1.0443902135080234 72-73 0.08370063204728168 74-75 0.10615996159156445 76-77 0.4755696327891772 78-79 0.9339455580201701 80-81 0.3045428584746772 82-83 0.5057508282564419 84-85 0.06580850368915284 86-87 0.1002351559291139 88-89 0.08156613252385578 90-91 0.08980592847825722 92-93 0.17636302312306473 94-95 0.4713163285917624 96-97 0.3855518238435211 98-99 0.06486681272293553 100-101 0.23240933046243145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6371517.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.47230392220806 #Duplication Level Percentage of deduplicated Percentage of total 1 98.21516837942183 95.73258742049843 2 1.353729075958575 2.639021838403282 3 0.2353929633848651 0.6883288340459628 4 0.11241720455791594 0.4383025571501686 5 0.03856724469361162 0.1879619098108939 6 0.015007789201454331 0.08777062741467533 7 0.020227261644641108 0.13801184561783358 8 0.004829989352323714 0.03766321520725808 9 0.001432005868058893 0.012562282007083961 >10 0.0032280859167033364 0.037789469844425036 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.708454831086537E-5 0.0 0.0 2 4.708454831086537E-5 1.5694849436955124E-5 0.0 3 9.416909662173074E-5 3.138969887391025E-5 0.0 4 9.416909662173074E-5 3.138969887391025E-5 0.0 5 1.25558795495641E-4 3.138969887391025E-5 0.0 6 1.7264334380650637E-4 3.138969887391025E-5 0.0 7 1.7264334380650637E-4 3.138969887391025E-5 0.0 8 1.883381932434615E-4 3.138969887391025E-5 0.0 9 2.040330426804166E-4 4.708454831086537E-5 0.0 10-11 2.040330426804166E-4 4.708454831086537E-5 7.847424718477562E-6 12-13 2.1972789211737175E-4 5.493197302934294E-5 1.5694849436955124E-5 14-15 2.5896501570975955E-4 6.27793977478205E-5 1.5694849436955124E-5 16-17 3.2174441345758E-4 7.847424718477561E-5 3.9237123592387806E-5 18-19 3.6098153704996783E-4 1.0986394605868586E-4 4.708454831086537E-5 20-21 3.923712359238781E-4 1.0986394605868586E-4 4.708454831086537E-5 22-23 4.0021866064235564E-4 1.412536449325961E-4 6.27793977478205E-5 24-25 4.2376093479778835E-4 1.412536449325961E-4 6.27793977478205E-5 26-27 4.708454831086537E-4 1.412536449325961E-4 7.847424718477561E-5 28-29 5.414723055749517E-4 1.412536449325961E-4 7.847424718477561E-5 30-31 6.356414021966826E-4 1.4910106965107365E-4 7.847424718477561E-5 32-33 8.475218695955767E-4 1.5694849436955123E-4 1.020165213402083E-4 34-35 0.001310519927985753 1.7264334380650637E-4 1.0986394605868586E-4 36-37 0.0019069242065900475 1.7264334380650637E-4 1.0986394605868586E-4 38-39 0.00257395530766064 1.8049076852498394E-4 1.0986394605868586E-4 40-41 0.003774611289587707 1.883381932434615E-4 1.0986394605868586E-4 42-43 0.0058149417163918735 1.883381932434615E-4 1.0986394605868586E-4 44-45 0.009275656017240478 1.883381932434615E-4 1.25558795495641E-4 46-47 0.014180296466288954 2.040330426804166E-4 1.412536449325961E-4 48-49 0.020418999117478617 2.040330426804166E-4 1.5694849436955123E-4 50-51 0.029663265435845184 2.1972789211737172E-4 1.647959190880288E-4 52-53 0.04163058813152347 2.275753168358493E-4 1.8049076852498394E-4 54-55 0.05931083602225341 2.3542274155432687E-4 1.883381932434615E-4 56-57 0.08188002951259488 2.982021393021473E-4 1.883381932434615E-4 58-59 0.1122338683236661 3.3743926289453517E-4 1.883381932434615E-4 60-61 0.1512983485722474 3.4528668761301274E-4 2.040330426804166E-4 62-63 0.1988929794898138 3.4528668761301274E-4 2.275753168358493E-4 64-65 0.2590748796558182 3.4528668761301274E-4 2.51117590991282E-4 66-67 0.3331781112724018 3.4528668761301274E-4 2.5896501570975955E-4 68-69 0.42285063353044494 3.4528668761301274E-4 2.825072898651922E-4 70-71 0.532141717584682 3.531341123314903E-4 2.9820213930214736E-4 72-73 0.652937126276207 3.688289617684454E-4 2.9820213930214736E-4 74-75 0.7964037449794139 3.923712359238781E-4 2.9820213930214736E-4 76-77 0.9620628807864752 3.923712359238781E-4 2.9820213930214736E-4 78-79 1.1516017300118637 4.0021866064235564E-4 2.9820213930214736E-4 80-81 1.3764069059220905 4.080660853608332E-4 2.9820213930214736E-4 82-83 1.628175833165006 4.159135100793108E-4 2.9820213930214736E-4 84-85 1.9124880307154482 4.316083595162659E-4 2.9820213930214736E-4 86-87 2.2371202964694277 4.3945578423474344E-4 2.9820213930214736E-4 88-89 2.5906389326121237 4.3945578423474344E-4 3.295918381760576E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGTGT 9285 0.0 6.5614944 94-95 CGTCGTG 12955 0.0 6.3809867 94-95 CGTGTAG 7010 0.0 6.17089 94-95 TCGTGTA 7780 0.0 6.1132493 94-95 GAGCGTC 16165 0.0 6.0806003 94-95 TGTAGGG 7040 0.0 6.0801554 92-93 GTGTAGG 7190 0.0 5.8903112 92-93 GCGTCGT 13875 0.0 5.8599496 94-95 AGCGTCG 14925 0.0 5.857313 92-93 AGAGCGT 17005 0.0 5.8068724 94-95 GTAGGGA 5230 0.0 5.759468 94-95 AAGAGCG 19155 0.0 5.7581005 94-95 CGGAAGA 23065 0.0 5.567523 94-95 GATCGGA 27105 0.0 5.531475 94-95 AGATCGG 29415 0.0 5.374264 94-95 GTCCTCA 3175 1.4297257E-9 5.351215 1 TCGGAAG 23920 0.0 5.311707 94-95 ATCGGAA 25660 0.0 5.1545243 94-95 GAAGAGC 22095 0.0 5.1251717 94-95 >>END_MODULE