FastQCFastQC Report
Thu 11 Sep 2014
LONLib32_CACTCA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib32_CACTCA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6371517
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC131750.07.095762394-95
CACGTCT124700.07.02123394-95
ACGTCTG109800.06.958361694-95
CGTCTGA100050.06.83012894-95
CACACGT142550.06.824488694-95
GCACACG153450.06.556203494-95
TCTGAAC104600.06.351551594-95
CTGAACT103550.06.27847294-95
GTCTAAC15751.0455715E-66.06985761
CAGTCAC53700.06.053405394-95
GAGCACA192700.05.934973794-95
GGTTGCG17006.506398E-75.9047221
AGAGCAC199950.05.87404594-95
CTCCAGT72850.05.862683394-95
AGCACAC177900.05.721828594-95
GTCTGAA116000.05.686437694-95
CGGAAGA243800.05.605859894-95
TCGGAAG255500.05.599894594-95
CCAGTCA59300.05.521764394-95
GATCGGA289650.05.488513594-95
Produced by FastQC (version 0.11.2)