##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib31_CACGAT_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2458182 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.729415885398232 27.0 22.0 33.0 2.0 33.0 2 24.710833453340722 27.0 15.0 33.0 2.0 33.0 3 25.62347865210957 33.0 22.0 33.0 6.0 33.0 4 27.726444583842856 33.0 22.0 37.0 2.0 37.0 5 27.798100791560593 33.0 15.0 37.0 6.0 37.0 6 28.83701450909656 33.0 22.0 37.0 15.0 37.0 7 28.512200886671533 33.0 27.0 37.0 6.0 37.0 8 28.27131229502128 33.0 22.0 37.0 6.0 37.0 9 29.01782943655108 33.0 27.0 37.0 15.0 37.0 10-11 28.631230925944458 33.0 24.5 37.0 10.5 37.0 12-13 28.92125216928608 33.0 27.0 37.0 10.5 37.0 14-15 29.244220322173057 33.0 24.5 37.0 6.0 40.0 16-17 30.092525899221457 33.0 27.0 37.0 15.0 40.0 18-19 30.398857977155473 33.0 27.0 37.0 15.0 40.0 20-21 30.40094732611336 33.0 27.0 37.0 15.0 40.0 22-23 30.400012692306753 33.0 27.0 37.0 15.0 40.0 24-25 29.997109855983 33.0 27.0 37.0 10.5 40.0 26-27 29.46800623387528 33.0 27.0 37.0 10.5 40.0 28-29 29.642290725422285 33.0 27.0 37.0 15.0 40.0 30-31 29.548669301133927 33.0 27.0 37.0 10.5 40.0 32-33 29.365881574269114 33.0 27.0 37.0 6.0 40.0 34-35 29.39857565469115 33.0 24.5 37.0 10.5 40.0 36-37 29.634815485590572 33.0 27.0 37.0 6.0 40.0 38-39 29.65393205222396 33.0 27.0 37.0 15.0 40.0 40-41 29.718803571094412 33.0 27.0 37.0 15.0 40.0 42-43 29.603875547050627 33.0 27.0 37.0 15.0 40.0 44-45 29.347312770169175 33.0 24.5 37.0 10.5 40.0 46-47 28.9682210267588 33.0 22.0 37.0 10.5 40.0 48-49 28.797652899581884 33.0 22.0 37.0 10.5 40.0 50-51 28.016210353830594 33.0 22.0 37.0 10.5 38.5 52-53 27.731948041276034 33.0 22.0 37.0 6.0 37.0 54-55 28.673835785958893 33.0 22.0 37.0 10.5 40.0 56-57 28.571883814949423 33.0 22.0 37.0 6.0 40.0 58-59 28.75355730373097 33.0 22.0 37.0 10.5 40.0 60-61 28.50992461095232 33.0 22.0 37.0 10.5 40.0 62-63 28.37221145545773 33.0 22.0 37.0 10.5 40.0 64-65 28.324066118782092 33.0 22.0 37.0 10.5 40.0 66-67 28.194757345062328 33.0 22.0 37.0 10.5 40.0 68-69 27.908772011185505 33.0 22.0 37.0 6.0 37.0 70-71 27.808521907653706 33.0 22.0 37.0 6.0 37.0 72-73 27.57397418091907 33.0 22.0 37.0 6.0 37.0 74-75 27.516846392984732 33.0 22.0 37.0 6.0 37.0 76-77 27.130368499972747 27.0 22.0 35.0 6.0 37.0 78-79 26.583149050802582 27.0 22.0 33.0 6.0 37.0 80-81 26.46296734741366 27.0 22.0 33.0 6.0 37.0 82-83 26.048466712391516 27.0 22.0 33.0 6.0 37.0 84-85 26.15062920483512 27.0 22.0 33.0 6.0 37.0 86-87 25.85597791375903 27.0 22.0 33.0 6.0 37.0 88-89 25.497646838191802 27.0 22.0 33.0 6.0 37.0 90-91 25.370777468877407 27.0 22.0 33.0 6.0 37.0 92-93 25.262860723900836 27.0 18.5 33.0 6.0 37.0 94-95 25.05816452972156 27.0 15.0 33.0 6.0 37.0 96-97 24.936843773162444 27.0 15.0 33.0 6.0 37.0 98-99 24.714465405734806 27.0 15.0 33.0 6.0 37.0 100-101 24.25435138651247 27.0 15.0 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.228228188574548 2306 2 8.3426327224062 2306 3 7.364986636933249 2306 4 8.889103992809773 2306 5 8.820143839012971 2306 6 7.87016822108167 2306 7 8.148715716487459 2306 8 8.49367021005322 2306 9 7.448923055856188 2306 10-11 7.745388982856593 2306 12-13 7.380236389225377 2306 14-15 8.016037582312421 2306 16-17 6.734681164031322 2306 18-19 6.171757476900922 2306 20-21 6.039409675581641 2306 22-23 5.71267361385442 2306 24-25 5.82988159677889 2306 26-27 6.413949608945881 2306 28-29 5.509749159728955 2306 30-31 5.53480684344812 2306 32-33 5.508811963800895 2306 34-35 4.813434830497588 2306 36-37 4.757978870848522 2306 38-39 4.523203911519442 2306 40-41 3.7422977251325875 2306 42-43 3.493550704260901 2306 44-45 3.3057891654891236 2306 46-47 3.5562585324540947 2306 48-49 3.3382556587731003 2306 50-51 3.1564420926443297 2306 52-53 3.7835595894476164 2306 54-55 3.2281353778658612 2306 56-57 3.477930320221059 2306 58-59 2.9898123819871074 2306 60-61 3.156279690398552 2306 62-63 2.6853779350371525 2306 64-65 2.1893097898856198 2306 66-67 2.065537114803778 2306 68-69 2.3265471131578437 2306 70-71 2.3248033077935553 2306 72-73 1.5521111165043031 2306 74-75 1.2492722005778099 2306 76-77 1.595120302476488 2306 78-79 1.7574393705122056 2306 80-81 1.5156763003761462 2306 82-83 1.4513807109048322 2306 84-85 1.1120573591523062 2306 86-87 1.0205720148300443 2306 88-89 0.9374600337660475 2306 90-91 0.8968873042373744 2306 92-93 0.936448268117303 2306 94-95 1.207028655333442 2306 96-97 1.0346933684122313 2306 98-99 0.718802619623208 2306 100-101 0.4743416096273698 2307 1 8.453376195572087 2307 2 8.479725634775924 2307 3 7.665133370276447 2307 4 9.186086592426449 2307 5 9.131414610119897 2307 6 7.722927402421959 2307 7 8.196974148375382 2307 8 8.591340959359929 2307 9 7.67000129294016 2307 10-11 7.890965112643752 2307 12-13 7.465432417347383 2307 14-15 8.313647199695502 2307 16-17 7.041524740530761 2307 18-19 6.526482790256473 2307 20-21 6.473201899662289 2307 22-23 6.141896502806333 2307 24-25 6.307969062089661 2307 26-27 7.077956144273639 2307 28-29 6.253580752447824 2307 30-31 6.024116789026312 2307 32-33 6.067850285429046 2307 34-35 5.720373037249427 2307 36-37 5.536480850535984 2307 38-39 5.278227894204161 2307 40-41 4.671243058494454 2307 42-43 4.437885946252091 2307 44-45 4.345449838186298 2307 46-47 4.638763020561093 2307 48-49 4.342327589194706 2307 50-51 4.127359424149272 2307 52-53 4.813097585013278 2307 54-55 4.308266460767548 2307 56-57 4.685568611727536 2307 58-59 4.401440267650514 2307 60-61 4.418130599731999 2307 62-63 4.0630725328679915 2307 64-65 3.8742381449995484 2307 66-67 3.622856630317518 2307 68-69 3.6590290378945234 2307 70-71 3.3537039360623275 2307 72-73 2.8739281462838413 2307 74-75 2.6000103137587445 2307 76-77 2.6762500453753333 2307 78-79 2.9005304804606666 2307 80-81 2.864466994329238 2307 82-83 2.8796402920436535 2307 84-85 2.114817826343355 2307 86-87 2.1609456412434938 2307 88-89 2.3297101659577812 2307 90-91 2.113213486451695 2307 92-93 1.9008465279465518 2307 94-95 2.234183662795207 2307 96-97 1.906697763024983 2307 98-99 1.7719758936926446 2307 100-101 1.4757244393172364 1101 1 7.9265858341720765 1101 2 7.707057706836721 1101 3 7.155808454407033 1101 4 8.670564797447064 1101 5 8.429542839510184 1101 6 7.44282401284125 1101 7 7.047466431348994 1101 8 8.023194587808216 1101 9 7.287879505637559 1101 10-11 7.422784668733353 1101 12-13 7.122663990890938 1101 14-15 7.519282937864553 1101 16-17 6.8176813677984 1101 18-19 5.875014382942169 1101 20-21 5.637759048111082 1101 22-23 5.181386394987484 1101 24-25 5.872172566633459 1101 26-27 6.419566686826325 1101 28-29 5.7892777379761 1101 30-31 5.491875117037548 1101 32-33 5.548299908941573 1101 34-35 5.194647712753611 1101 36-37 5.240277115333203 1101 38-39 4.787051222834545 1101 40-41 4.3521618048123685 1101 42-43 4.075244917974064 1101 44-45 4.115472178028156 1101 46-47 4.485412184797582 1101 48-49 4.112106623652078 1101 50-51 3.559011901885288 1101 52-53 4.494290069898874 1101 54-55 4.219738127331706 1101 56-57 4.372969234755807 1101 58-59 3.883353743282605 1101 60-61 4.187901185468146 1101 62-63 3.852281794103625 1101 64-65 3.589179927262805 1101 66-67 3.3265338303992102 1101 68-69 3.5856022333641704 1101 70-71 3.290087502029568 1101 72-73 2.912828691245924 1101 74-75 2.231075824147055 1101 76-77 2.597908824256578 1101 78-79 3.1375566330414415 1101 80-81 2.715053974127688 1101 82-83 2.7615907037062613 1101 84-85 2.21871027494036 1101 86-87 2.1998240416625805 1101 88-89 2.2784405330417314 1101 90-91 2.2847987614582834 1101 92-93 1.9643572195631407 1101 94-95 2.0882802236036078 1101 96-97 2.0126158236624683 1101 98-99 1.8829272221926914 1101 100-101 1.6051601469143613 2201 1 7.083886392124182 2201 2 7.747751699934927 2201 3 6.172162563394398 2201 4 8.32862387457319 2201 5 3.851328746163489 2201 6 5.642400762754228 2201 7 6.67458434924238 2201 8 7.716252836480269 2201 9 6.484080139797424 2201 10-11 4.160763260106794 2201 12-13 6.364195833049859 2201 14-15 7.112923926102788 2201 16-17 5.949230594081669 2201 18-19 2.1482248211130113 2201 20-21 4.8790096665011795 2201 22-23 5.030727685485402 2201 24-25 5.344328570346967 2201 26-27 5.994398441626519 2201 28-29 4.973548383139224 2201 30-31 5.091046469843153 2201 32-33 5.29293647795938 2201 34-35 4.879163049596816 2201 36-37 4.96318209839237 2201 38-39 4.2779148567906695 2201 40-41 4.033191089886003 2201 42-43 4.048024685197955 2201 44-45 3.721764462847993 2201 46-47 4.211963921477292 2201 48-49 3.8829667413723072 2201 50-51 3.6078865973215777 2201 52-53 4.368273979829443 2201 54-55 4.100700792752953 2201 56-57 4.322438525252572 2201 58-59 3.7960750248318575 2201 60-61 3.761015449294341 2201 62-63 3.5303977568597062 2201 64-65 3.5160764460354876 2201 66-67 3.085744664325407 2201 68-69 3.3507100938644534 2201 70-71 3.1708408455561248 2201 72-73 2.5170758585963995 2201 74-75 2.1508813446874804 2201 76-77 2.5492128374325915 2201 78-79 2.881596511276399 2201 80-81 2.3031148154514973 2201 82-83 2.5988646030684563 2201 84-85 2.0476473835637563 2201 86-87 1.9814355548889289 2201 88-89 2.1910158650518987 2201 90-91 2.016113617315323 2201 92-93 1.8873853424049472 2201 94-95 1.9563607053508143 2201 96-97 1.9923565184316274 2201 98-99 1.792269830921434 2201 100-101 1.51782910693073 2304 1 1.4400942421271488 2304 2 4.5457562749943 2304 3 5.77285990105937 2304 4 4.392691089872486 2304 5 6.568072964143536 2304 6 6.247678621152083 2304 7 7.108560406639718 2304 8 6.487776836817314 2304 9 5.901391246955551 2304 10-11 6.690940785517721 2304 12-13 6.560442786391118 2304 14-15 6.8668615667328154 2304 16-17 5.587968255308816 2304 18-19 5.383753387147095 2304 20-21 5.043600626233786 2304 22-23 4.567632826341679 2304 24-25 4.731017441343404 2304 26-27 5.732780178214195 2304 28-29 5.013978441841047 2304 30-31 4.965116478238034 2304 32-33 5.010120335427246 2304 34-35 4.775975721824704 2304 36-37 4.936923879045413 2304 38-39 4.538292221169495 2304 40-41 3.903486128162335 2304 42-43 3.25010281073434 2304 44-45 3.2844183561735854 2304 46-47 4.233189497494742 2304 48-49 3.85289610258042 2304 50-51 3.439748094108733 2304 52-53 4.050673654644459 2304 54-55 3.769439655479328 2304 56-57 4.3942292740206454 2304 58-59 3.76447286462988 2304 60-61 3.84399652490141 2304 62-63 3.5058091533138764 2304 64-65 3.4424834484353646 2304 66-67 3.1219534817692995 2304 68-69 3.4046238605489094 2304 70-71 3.2933742612779753 2304 72-73 2.9993322897380104 2304 74-75 2.3747943779892964 2304 76-77 2.6245222624918085 2304 78-79 2.94596254099244 2304 80-81 2.7569269228187174 2304 82-83 2.960317309250403 2304 84-85 2.496538805483471 2304 86-87 2.461684156000551 2304 88-89 2.614509231839964 2304 90-91 2.5200890973688743 2304 92-93 2.3203580699330075 2304 94-95 2.6848636217171062 2304 96-97 2.5857629403669478 2304 98-99 2.2031681310340936 2304 100-101 1.7038266477583015 1102 1 7.02963827842315 1102 2 7.310820163511263 1102 3 6.013537695306706 1102 4 5.956146298400824 1102 5 6.412373397339195 1102 6 5.832251836180717 1102 7 6.744332450213122 1102 8 6.172229209387318 1102 9 4.839952642449635 1102 10-11 5.973490768488848 1102 12-13 5.909002180659453 1102 14-15 6.123913990850781 1102 16-17 5.216370390346036 1102 18-19 5.287678480816147 1102 20-21 5.453361517689647 1102 22-23 5.125299627790355 1102 24-25 1.7742441994950582 1102 26-27 5.321138780440034 1102 28-29 4.731286466161684 1102 30-31 4.714809451044534 1102 32-33 5.000722545469415 1102 34-35 4.827497929791843 1102 36-37 4.700333988229094 1102 38-39 3.7673651102812933 1102 40-41 3.906358327958479 1102 42-43 4.053488326985995 1102 44-45 -3.5252462365451933 1102 46-47 1.354681918774908 1102 48-49 -0.9655535841231746 1102 50-51 2.494153728010321 1102 52-53 -0.15352367544153012 1102 54-55 2.568093482587777 1102 56-57 -0.6750895925966347 1102 58-59 2.7643580658320097 1102 60-61 3.771534493341509 1102 62-63 3.4094907320513457 1102 64-65 3.052720512694602 1102 66-67 2.8825874028328364 1102 68-69 3.3611229847050765 1102 70-71 3.261353060177548 1102 72-73 2.7371154062821397 1102 74-75 2.4383988326439976 1102 76-77 2.6305748682784227 1102 78-79 -1.6127130494460502 1102 80-81 1.280033262952049 1102 82-83 2.389043915567264 1102 84-85 2.1114751107327336 1102 86-87 2.1338912722034884 1102 88-89 2.1722472090559286 1102 90-91 1.9176777792479989 1102 92-93 2.075011124847176 1102 94-95 2.376399046796113 1102 96-97 2.199148064985284 1102 98-99 2.272060506917601 1102 100-101 1.917605179321999 2202 1 -20.477697464719526 2202 2 -7.509807101793463 2202 3 -0.6582617878835393 2202 4 1.456565589233172 2202 5 1.3071916223659592 2202 6 1.7136248222117025 2202 7 4.021089277690038 2202 8 2.8681323206358016 2202 9 3.2597223787309453 2202 10-11 1.9017336725169791 2202 12-13 1.4164503196228928 2202 14-15 2.6938670894741676 2202 16-17 1.8011009849493576 2202 18-19 1.6365992837998817 2202 20-21 1.2218709408630701 2202 22-23 1.4360972843819155 2202 24-25 2.364277250196963 2202 26-27 3.438752094058188 2202 28-29 -0.7956168396137571 2202 30-31 1.1000085040986427 2202 32-33 1.6676387523303724 2202 34-35 1.6050496911493006 2202 36-37 3.1994133864916385 2202 38-39 2.5720042644427643 2202 40-41 1.917246201076928 2202 42-43 0.8055628045439498 2202 44-45 0.9392043647419044 2202 46-47 -1.046782153406756 2202 48-49 0.9588936563956061 2202 50-51 1.7394347278355617 2202 52-53 1.7439635893734113 2202 54-55 -0.5499326524392529 2202 56-57 1.2937053515017176 2202 58-59 2.082593338161697 2202 60-61 2.201054289416394 2202 62-63 -1.9886767940305639 2202 64-65 0.03887658506484826 2202 66-67 0.5090990613725559 2202 68-69 1.4238095880061934 2202 70-71 -2.4842982516688217 2202 72-73 -4.618716684579439 2202 74-75 -1.2207237180614285 2202 76-77 -0.08317996456139198 2202 78-79 1.5872435026833145 2202 80-81 1.7582577110755189 2202 82-83 -0.5047395904237817 2202 84-85 -0.27180754915212546 2202 86-87 0.7221017224936865 2202 88-89 1.2970943376897779 2202 90-91 1.4083551734219775 2202 92-93 1.4794081226570661 2202 94-95 1.838681280587533 2202 96-97 1.2251492610234216 2202 98-99 1.2557564768888128 2202 100-101 1.0729643858696996 2305 1 5.81749817668711 2305 2 6.990680172987474 2305 3 6.716612787691332 2305 4 7.792169788618203 2305 5 7.844437615672433 2305 6 6.115819900024974 2305 7 2.763226684521534 2305 8 5.934274859698306 2305 9 6.3849568481893435 2305 10-11 4.6390267246408925 2305 12-13 5.254952943753459 2305 14-15 5.6448631505295594 2305 16-17 4.924553900455237 2305 18-19 4.375427050985756 2305 20-21 5.072758713603395 2305 22-23 5.070830927982648 2305 24-25 5.232853601964074 2305 26-27 6.00914321776661 2305 28-29 4.967092792348261 2305 30-31 5.413670009947172 2305 32-33 5.214462534392425 2305 34-35 5.08896561341869 2305 36-37 4.619211314273816 2305 38-39 4.539708372361904 2305 40-41 3.9375707141805094 2305 42-43 3.887539089900965 2305 44-45 3.0241047515219215 2305 46-47 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48-49 -19.327502559616697 1209 50-51 -21.436991763947418 1209 52-53 -24.056245342462578 1209 54-55 -21.16825965010812 1209 56-57 -24.88506195066595 1209 58-59 -25.15271614212036 1209 60-61 -24.891659212803507 1209 62-63 -25.021191811006275 1209 64-65 -24.896354545778713 1209 66-67 -12.896375404126282 1209 68-69 -24.58742049817819 1209 70-71 -24.411302691470336 1209 72-73 -14.850211869152016 1209 74-75 -15.772682615236656 1209 76-77 -13.225671154279881 1209 78-79 -13.864341058285767 1209 80-81 -23.500426018357754 1209 82-83 -15.055284651023689 1209 84-85 -12.638686049485838 1209 86-87 -12.92975754514864 1209 88-89 -10.48865219059179 1209 90-91 -12.658032272612026 1209 92-93 -16.92294526232886 1209 94-95 -22.233530785225998 1209 96-97 -18.009396070414052 1209 98-99 -9.889809041620031 1209 100-101 -5.496824651012574 1212 1 -20.477697464719526 1212 2 -20.232450089518718 1212 3 -21.171450610030313 1212 4 -23.126354233174766 1212 5 -23.32247669834441 1212 6 -24.348271234199892 1212 7 -23.941825321422 1212 8 -23.5573051303958 1212 9 -24.44986236924503 1212 10-11 -24.17152305247649 1212 12-13 -24.45257553569181 1212 14-15 -24.77923293664231 1212 16-17 -25.80414842742985 1212 18-19 -20.46196775472973 1212 20-21 -26.194948071934004 1212 22-23 -26.377039304805862 1212 24-25 -22.449254030544004 1212 26-27 -25.103164855537006 1212 28-29 -19.97641192300247 1212 30-31 -14.749574265266933 1212 32-33 -25.195641477494647 1212 34-35 -21.304769104059517 1212 36-37 -25.622863970520637 1212 38-39 -25.87732976533002 1212 40-41 -20.468016036937538 1212 42-43 -16.725451618995855 1212 44-45 -14.30672851620022 1212 46-47 -17.19019828314471 1212 48-49 -19.83527801102236 1212 50-51 -11.879992664670983 1212 52-53 -4.868533955765848 1212 54-55 -3.9692236032704926 1212 56-57 -4.067700056641147 1212 58-59 -2.8082967509140495 1212 60-61 -13.668434861056044 1212 62-63 -11.591090345391844 1212 64-65 -15.970762663027868 1212 66-67 -12.143353605820757 1212 68-69 -7.8698331250102065 1212 70-71 -15.006567629463571 1212 72-73 -11.448024880531356 1212 74-75 -8.00286619979829 1212 76-77 -18.587497575106678 1212 78-79 -9.615327778081205 1212 80-81 -7.512827371232614 1212 82-83 -5.143155901239922 1212 84-85 -4.447825112852634 1212 86-87 -3.445622607612723 1212 88-89 -3.02085952005579 1212 90-91 -3.451664176916612 1212 92-93 -3.437162974968217 1212 94-95 -3.0255262756431343 1212 96-97 -12.769290608211632 1212 98-99 -9.989491104098036 1212 100-101 -6.341414046334776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 400.0 3 891.0 4 479.0 5 2627.0 6 3411.0 7 3743.0 8 8898.0 9 15800.0 10 19485.0 11 22289.0 12 27821.0 13 33871.0 14 38673.0 15 41205.0 16 44099.0 17 48217.0 18 54348.0 19 59632.0 20 64015.0 21 70911.0 22 78129.0 23 84054.0 24 87620.0 25 89699.0 26 94002.0 27 98030.0 28 99596.0 29 100890.0 30 104053.0 31 109253.0 32 117834.0 33 134708.0 34 151502.0 35 163804.0 36 171059.0 37 156806.0 38 56162.0 39 166.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.54853268034347 17.978557402961723 13.788994407855848 20.68391550883896 2 23.57748797973688 28.604251454218833 25.70033680494005 22.117923761104237 3 20.218227052696243 27.313871519239324 28.22598079220014 24.24192063586429 4 23.18369408654857 27.035628472415503 26.418029027403932 23.362648413631995 5 22.248002039328632 27.782498445906363 26.629070271865764 23.340429242899248 6 22.033375053939807 27.60872225454436 26.786046281964033 23.5718564095518 7 23.427393704307473 26.50455850421815 27.73338051433596 22.33466727713842 8 22.53956937889832 26.899542524940657 27.730325545504897 22.830562550656126 9 22.69207749224781 26.86345791690065 27.495191000113 22.949273590738535 10-11 23.689378043727444 27.056244968496195 26.215347603251505 23.03902938452486 12-13 23.130846593759838 27.285504476367596 26.56127085220198 23.02237807767059 14-15 22.923814283576778 27.43634004571257 26.915897733370837 22.723947937339815 16-17 23.37287605571841 27.31232386415029 26.527429826098782 22.78737025403252 18-19 23.31850763698843 27.355108636013565 26.490190123510647 22.83619360348736 20-21 23.116435517490373 27.479475459841403 26.655975698121853 22.74811332454637 22-23 23.26518544773902 27.32296041923937 26.56492954407488 22.846924588946735 24-25 23.129967619107003 27.22849299509357 26.778021100242583 22.863518285556843 26-27 22.99919755389947 27.4039381062442 26.796769474406307 22.80009486545002 28-29 23.33620373801209 27.304710706678453 26.652576843700253 22.7065087116092 30-31 23.247394192602712 27.125107667462554 26.612838308795588 23.014659831139145 32-33 22.96604541049652 27.535951357011296 26.723319514048256 22.77468371844393 34-35 23.288985889411933 27.226491932923558 26.69785844923388 22.78666372843063 36-37 23.250569068552284 27.23600997424641 26.63538651043787 22.878034446763433 38-39 22.971603793861522 27.443305977959763 26.927922654135422 22.657167574043292 40-41 23.4038104481304 27.196498329137142 26.530355209103785 22.869336013628676 42-43 23.219864965394542 27.409521312162134 26.54056509664921 22.83004862579411 44-45 22.98237988909671 27.265628218543757 26.9764639481902 22.775527944169337 46-47 23.366673400645638 27.232911841221018 26.53072961975007 22.869685138383268 48-49 23.145620759550077 27.15168823113558 26.764069382188318 22.93862162712603 50-51 23.23746074313382 27.242942444205443 26.699939073839694 22.819657738821043 52-53 23.14436152255762 27.328742297934035 26.658126078435217 22.868770101073128 54-55 23.032923867501534 27.13741267678069 26.81853223521572 23.011131220502058 56-57 22.9619838694848 27.32864951456874 26.904409675658524 22.804956940287937 58-59 23.201367568557163 27.22294979396794 26.71672378644408 22.858958851030824 60-61 23.210172426095003 26.980186279738007 26.694637910149698 23.115003384017292 62-63 23.006162403302497 27.335664640501918 26.818068606084474 22.84010435011112 64-65 23.254113691903846 27.141387268427845 26.68449345438085 22.920005585287463 66-67 23.31706812160329 27.106451971872243 26.598551063791486 22.977928842732982 68-69 23.059665271125713 27.264251014461966 26.8629159376081 22.813167776804217 70-71 23.439749289781776 27.051187902393643 26.53427889374856 22.974783914076024 72-73 23.218514961253785 27.217377390516962 26.6334664842102 22.930641164019054 74-75 23.08488452640371 27.19014181153722 26.760101369002797 22.96487229305627 76-77 23.545907435785253 27.146024240955413 26.427210618562665 22.880857704696666 78-79 23.31651375205886 27.122579877627288 26.598937084510194 22.961969285803658 80-81 23.3289683832501 27.169784454154456 26.605292163673592 22.89595499892185 82-83 23.407433238746115 27.171046418597694 26.50740964985832 22.914110692797866 84-85 23.428690175881513 26.8894531744523 26.52636960803909 23.155487041627097 86-87 23.34628520143874 27.136070762644053 26.58001049777279 22.937633538144418 88-89 23.514782504257088 27.05067487447569 26.48007743676964 22.954465184497582 90-91 23.63912752534532 26.915355724116058 26.317112749847265 23.12840400069136 92-93 23.45589916222528 27.170465700706725 26.424316860506575 22.94931827656142 94-95 23.73132309387361 26.986451235604974 26.27928199272642 23.002943677794992 96-97 23.732273387857806 27.00145492053472 26.182975347202515 23.083296344404967 98-99 23.581687709042267 27.13372456663005 26.315334199981642 22.96925352434604 100-101 24.132077137282156 26.842944029579176 25.877739230384545 23.147239602754127 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 171.0 1 173.0 2 196.5 3 215.0 4 238.5 5 295.5 6 351.5 7 451.5 8 563.0 9 692.0 10 909.0 11 1208.5 12 1622.0 13 2086.0 14 2660.5 15 3348.5 16 4213.0 17 5271.5 18 6571.5 19 8224.0 20 10200.0 21 12562.5 22 15321.0 23 18629.5 24 22262.5 25 26145.5 26 30330.5 27 34785.5 28 39528.5 29 44254.0 30 48779.0 31 52899.5 32 57057.0 33 61078.5 34 64839.0 35 68287.5 36 70581.5 37 72707.0 38 74293.5 39 74246.5 40 72951.0 41 70967.5 42 68375.5 43 65450.0 44 62676.0 45 59380.5 46 56573.0 47 54529.0 48 52145.0 49 49818.5 50 47626.0 51 45345.0 52 43247.5 53 41569.5 54 40420.0 55 39676.5 56 39022.5 57 38735.0 58 38343.0 59 37903.5 60 37644.5 61 37147.0 62 36582.0 63 35783.0 64 34909.0 65 34139.0 66 33160.0 67 31722.0 68 29906.0 69 27827.5 70 25414.5 71 22756.0 72 20114.0 73 17242.0 74 14338.0 75 11719.5 76 9375.5 77 7359.0 78 5799.5 79 4619.0 80 3684.5 81 2968.0 82 2330.0 83 1849.5 84 1495.5 85 1186.0 86 926.0 87 764.5 88 621.5 89 475.0 90 388.5 91 305.5 92 217.5 93 165.0 94 119.0 95 76.5 96 59.0 97 43.0 98 21.5 99 7.0 100 2.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.628974583655726 2 10.958423745678717 3 9.675198988520785 4 11.571275031710426 5 9.037247852274568 6 5.255794729600981 7 9.63919677224876 8 9.955650151209309 9 5.319988511835169 10-11 6.288041324849014 12-13 5.3388845903191875 14-15 7.288272389920682 16-17 2.628385530444857 18-19 3.020850368280298 20-21 3.0707856456519496 22-23 3.1329047238975796 24-25 3.4399405739688924 26-27 4.286745245063221 28-29 1.7381544572370962 30-31 2.2117361529780952 32-33 3.0084631650544997 34-35 1.9759724869842836 36-37 3.020219820989658 38-39 1.3896855481001815 40-41 1.1634207719363334 42-43 0.5572207428091167 44-45 1.254667066962495 46-47 1.329112327728378 48-49 1.2266178826466063 50-51 1.580843078340009 52-53 2.465683989224557 54-55 1.7179159232310708 56-57 1.7748075610349436 58-59 1.562658908087359 60-61 1.3647891002374926 62-63 0.6126682239150723 64-65 1.2356692873025674 66-67 0.5657026208799837 68-69 0.806307262847096 70-71 1.0078586532648925 72-73 0.08424925412357588 74-75 0.10572854247569953 76-77 0.46263864921311765 78-79 0.835861624566448 80-81 0.2936519753215995 82-83 0.4928438984582916 84-85 0.06447854552673479 86-87 0.09856877969165831 88-89 0.08073039343710108 90-91 0.0877274343396868 92-93 0.17008504659134271 94-95 0.45737052830099645 96-97 0.37633503133616636 98-99 0.06474296858409996 100-101 0.2296615954392311 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2458182.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.20731687623811 #Duplication Level Percentage of deduplicated Percentage of total 1 98.69855210733209 96.92919982030661 2 0.9943305014932865 1.9530106127971987 3 0.15747928115982793 0.4639685299891624 4 0.10551235438482232 0.41448340885712703 5 0.03074444331920248 0.150966464361623 6 0.010692513038241914 0.06300498096899587 7 0.0 0.0 8 0.0016090931263580265 0.012641977483489551 9 0.0 0.0 >10 0.0010797061461867007 0.012724205235813501 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.220414110916116E-4 0.0 0.0 2 1.220414110916116E-4 4.068047036387054E-5 0.0 3 1.220414110916116E-4 4.068047036387054E-5 0.0 4 1.220414110916116E-4 4.068047036387054E-5 0.0 5 1.220414110916116E-4 4.068047036387054E-5 0.0 6 1.220414110916116E-4 4.068047036387054E-5 0.0 7 1.6272188145548215E-4 4.068047036387054E-5 0.0 8 1.6272188145548215E-4 4.068047036387054E-5 0.0 9 1.6272188145548215E-4 8.136094072774107E-5 0.0 10-11 1.6272188145548215E-4 1.0170117590967633E-4 0.0 12-13 1.6272188145548215E-4 1.220414110916116E-4 0.0 14-15 2.0340235181935266E-4 1.6272188145548215E-4 0.0 16-17 2.0340235181935266E-4 1.6272188145548215E-4 0.0 18-19 2.0340235181935266E-4 1.6272188145548215E-4 0.0 20-21 2.0340235181935266E-4 1.6272188145548215E-4 0.0 22-23 2.440828221832232E-4 1.6272188145548215E-4 0.0 24-25 2.440828221832232E-4 2.0340235181935266E-4 2.034023518193527E-5 26-27 2.644230573651585E-4 2.0340235181935266E-4 4.068047036387054E-5 28-29 4.474851740025759E-4 2.0340235181935266E-4 4.068047036387054E-5 30-31 7.322484665496697E-4 2.0340235181935266E-4 4.068047036387054E-5 32-33 0.0010373519942786986 2.0340235181935266E-4 4.068047036387054E-5 34-35 0.0016882395201006273 2.0340235181935266E-4 4.068047036387054E-5 36-37 0.0024611684570141675 2.0340235181935266E-4 4.068047036387054E-5 38-39 0.0033764790402012546 2.0340235181935266E-4 6.10207055458058E-5 40-41 0.005227440441757363 2.0340235181935266E-4 8.136094072774107E-5 42-43 0.007119082313677343 2.2374258700128794E-4 8.136094072774107E-5 44-45 0.00907174489114313 2.644230573651585E-4 8.136094072774107E-5 46-47 0.012651626283163736 2.8476329254709375E-4 8.136094072774107E-5 48-49 0.017106137788007562 3.05103527729029E-4 1.0170117590967633E-4 50-51 0.02235391846494686 3.254437629109643E-4 1.220414110916116E-4 52-53 0.029086536310167433 3.4578399809289957E-4 1.220414110916116E-4 54-55 0.0382396421420383 3.6612423327483484E-4 1.4238164627354688E-4 56-57 0.049182688669919476 3.6612423327483484E-4 1.6272188145548215E-4 58-59 0.06240384153817739 3.6612423327483484E-4 1.6272188145548215E-4 60-61 0.07678438781180563 3.6612423327483484E-4 1.6272188145548215E-4 62-63 0.09443971194972545 3.6612423327483484E-4 1.6272188145548215E-4 64-65 0.11541049442230071 3.6612423327483484E-4 1.6272188145548215E-4 66-67 0.142340965803183 3.6612423327483484E-4 1.6272188145548215E-4 68-69 0.17439717644991298 3.6612423327483484E-4 1.6272188145548215E-4 70-71 0.20820264732228938 3.6612423327483484E-4 2.0340235181935266E-4 72-73 0.24442860618131612 3.6612423327483484E-4 2.2374258700128794E-4 74-75 0.2844581890193647 3.6612423327483484E-4 2.440828221832232E-4 76-77 0.33372223863001194 3.6612423327483484E-4 2.440828221832232E-4 78-79 0.38898665761933005 3.6612423327483484E-4 2.440828221832232E-4 80-81 0.4499463424595901 3.6612423327483484E-4 2.440828221832232E-4 82-83 0.518452254552348 3.6612423327483484E-4 2.440828221832232E-4 84-85 0.5952366423641536 3.6612423327483484E-4 2.440828221832232E-4 86-87 0.6837980263462998 3.6612423327483484E-4 2.644230573651585E-4 88-89 0.7805972055771297 3.6612423327483484E-4 3.254437629109643E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCCTA 515 7.9941156E-4 9.85023 1 GGTAGCA 685 0.0011545854 8.2284975 1 GCTTATA 760 0.0032084186 7.4164743 1 GGTTCCA 1100 0.0025786615 6.148932 1 GAGGCAC 1215 0.0011849158 6.030845 1 GATTCAT 1220 0.0012447975 6.0061293 1 GTTGCCC 1725 1.2593144E-5 5.881587 1 TAGATCG 835 1.2136661E-6 5.709627 94-95 GTCACAA 1185 0.005908638 5.707869 1 GTGAAAG 1380 0.005377631 5.3097663 1 GGTCGGC 1710 4.1474708E-4 5.2739377 1 GTCCATT 1925 7.249139E-5 5.270513 1 GCAAACA 1545 0.0035979173 5.107527 1 >>END_MODULE