FastQCFastQC Report
Thu 11 Sep 2014
LONLib31_CACGAT_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib31_CACGAT_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458182
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTA5157.9941156E-49.850231
GGTAGCA6850.00115458548.22849751
GCTTATA7600.00320841867.41647431
GGTTCCA11000.00257866156.1489321
GAGGCAC12150.00118491586.0308451
GATTCAT12200.00124479756.00612931
GTTGCCC17251.2593144E-55.8815871
TAGATCG8351.2136661E-65.70962794-95
GTCACAA11850.0059086385.7078691
GTGAAAG13800.0053776315.30976631
GGTCGGC17104.1474708E-45.27393771
GTCCATT19257.249139E-55.2705131
GCAAACA15450.00359791735.1075271
Produced by FastQC (version 0.11.2)