##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib31_CACGAT_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2458182 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24249628383903 33.0 33.0 33.0 27.0 33.0 2 31.599607758904753 33.0 33.0 33.0 27.0 33.0 3 31.80748821690176 33.0 33.0 33.0 27.0 33.0 4 35.63825501935984 37.0 37.0 37.0 33.0 37.0 5 35.158891001561315 37.0 37.0 37.0 33.0 37.0 6 34.9587683092627 37.0 37.0 37.0 33.0 37.0 7 34.76235404864245 37.0 33.0 37.0 27.0 37.0 8 34.79693122803763 37.0 33.0 37.0 27.0 37.0 9 34.692278277198355 37.0 33.0 37.0 27.0 37.0 10-11 34.54887860215395 37.0 33.0 37.0 27.0 37.0 12-13 34.554822222276464 37.0 33.0 37.0 27.0 37.0 14-15 35.492471672154466 37.0 33.0 40.0 27.0 40.0 16-17 35.39894767759263 37.0 33.0 40.0 27.0 40.0 18-19 35.612585845962585 37.0 33.0 40.0 27.0 40.0 20-21 35.28383882885807 37.0 33.0 40.0 27.0 40.0 22-23 35.311161663375614 37.0 33.0 40.0 27.0 40.0 24-25 35.14810965990313 37.0 33.0 40.0 27.0 40.0 26-27 34.759551774441434 37.0 33.0 40.0 27.0 40.0 28-29 34.38987735651794 37.0 33.0 40.0 27.0 40.0 30-31 34.27655356682296 37.0 33.0 40.0 24.5 40.0 32-33 34.257834244982675 37.0 33.0 40.0 24.5 40.0 34-35 34.107254670321396 37.0 33.0 38.5 24.5 40.0 36-37 33.790266139773216 37.0 33.0 37.0 22.0 40.0 38-39 33.67129244295174 37.0 33.0 37.0 22.0 40.0 40-41 33.38031358133775 37.0 33.0 37.0 22.0 40.0 42-43 32.98727006381139 37.0 33.0 37.0 22.0 40.0 44-45 32.85512342047904 37.0 33.0 37.0 22.0 40.0 46-47 32.70117611307869 37.0 33.0 37.0 22.0 40.0 48-49 32.71780811998461 37.0 33.0 37.0 22.0 40.0 50-51 33.011804455487834 37.0 33.0 37.0 22.0 40.0 52-53 32.86237247689553 37.0 33.0 37.0 22.0 40.0 54-55 32.503664089965675 37.0 33.0 37.0 22.0 40.0 56-57 32.54322910183217 37.0 33.0 37.0 22.0 40.0 58-59 32.253409023416495 37.0 30.0 37.0 22.0 40.0 60-61 32.15164641999657 33.0 27.0 37.0 22.0 40.0 62-63 32.13025520486278 33.0 27.0 37.0 22.0 40.0 64-65 31.778267841844094 33.0 27.0 37.0 22.0 40.0 66-67 31.440065259610556 33.0 27.0 37.0 15.0 40.0 68-69 31.25536412682218 33.0 27.0 37.0 15.0 40.0 70-71 31.08951351039101 33.0 27.0 37.0 15.0 38.5 72-73 30.865398290281192 33.0 27.0 37.0 15.0 37.0 74-75 30.51069143781868 33.0 27.0 37.0 15.0 37.0 76-77 29.45016845782778 33.0 27.0 35.0 15.0 37.0 78-79 30.370423955589942 33.0 27.0 37.0 15.0 37.0 80-81 30.080543263273427 33.0 27.0 37.0 15.0 37.0 82-83 30.051708132270107 33.0 27.0 37.0 15.0 37.0 84-85 29.93990111391264 33.0 27.0 37.0 15.0 37.0 86-87 29.73357220905531 33.0 27.0 37.0 15.0 37.0 88-89 29.600861937806073 33.0 27.0 37.0 15.0 37.0 90-91 29.511599425917204 33.0 27.0 37.0 15.0 37.0 92-93 29.298119911381665 33.0 27.0 33.0 15.0 37.0 94-95 29.208612096256502 33.0 27.0 33.0 15.0 37.0 96-97 29.072886181739186 33.0 27.0 33.0 15.0 37.0 98-99 28.806935776114216 33.0 27.0 33.0 15.0 37.0 100-101 28.02033982837723 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.1554562204457426 2306 2 0.5528898903193884 2306 3 0.46502876610855637 2306 4 0.35359101382932323 2306 5 0.5923414871068076 2306 6 0.7237930465260263 2306 7 0.9553243415668007 2306 8 0.7857745060397434 2306 9 0.9931783296703927 2306 10-11 1.242232086028622 2306 12-13 1.3974588779859545 2306 14-15 1.4396743452164884 2306 16-17 1.4635788405367833 2306 18-19 1.2181965383469233 2306 20-21 1.4423831408428285 2306 22-23 1.155899141836862 2306 24-25 1.257706888977829 2306 26-27 1.4272045992795057 2306 28-29 1.6872780356791353 2306 30-31 1.5165132547998326 2306 32-33 1.2955937919750369 2306 34-35 1.203413815521614 2306 36-37 1.352936148236921 2306 38-39 1.1006643445082318 2306 40-41 1.3220691930866764 2306 42-43 1.2036838984519989 2306 44-45 1.0046086971028458 2306 46-47 1.048263478027522 2306 48-49 0.9377325127480844 2306 50-51 1.1069869810325201 2306 52-53 1.2220013778333652 2306 54-55 1.5800533238608345 2306 56-57 1.045282135281326 2306 58-59 1.0433762920679541 2306 60-61 0.7740772998763319 2306 62-63 0.5685513881419659 2306 64-65 0.8240591099189736 2306 66-67 0.9571907672363835 2306 68-69 0.7129773298187025 2306 70-71 0.7239645917320416 2306 72-73 0.5770580746918732 2306 74-75 0.7890448440329543 2306 76-77 0.6242426395533442 2306 78-79 0.6699900603033591 2306 80-81 1.0434958388845637 2306 82-83 1.038980069976855 2306 84-85 0.9713025291875468 2306 86-87 0.851657982580921 2306 88-89 0.47222289188073674 2306 90-91 0.15863094179198 2306 92-93 0.03853566373676642 2306 94-95 -0.03548430063729313 2306 96-97 0.14714658459156027 2306 98-99 0.2953110099929006 2306 100-101 0.22915397171369634 2307 1 0.759145970932849 2307 2 0.4890573316443465 2307 3 0.3755593750365982 2307 4 0.3582168009424791 2307 5 0.5209853773259567 2307 6 0.6367109471506041 2307 7 0.8259398779752942 2307 8 0.7624161384582919 2307 9 1.0200103411793506 2307 10-11 1.1954075868152216 2307 12-13 1.3932127827383596 2307 14-15 1.3646032418512988 2307 16-17 1.3538134974972351 2307 18-19 1.2748875551008254 2307 20-21 1.479079352573173 2307 22-23 1.278491612800856 2307 24-25 1.2151437131090645 2307 26-27 1.4164752027031255 2307 28-29 1.630758918702476 2307 30-31 1.6696691437488838 2307 32-33 1.291158721967257 2307 34-35 1.3435718267168042 2307 36-37 1.448610954002021 2307 38-39 1.0642534243806097 2307 40-41 1.2658469194614028 2307 42-43 1.318818800922834 2307 44-45 1.111080641825616 2307 46-47 1.123667560983506 2307 48-49 1.105075639130071 2307 50-51 1.083375555234504 2307 52-53 1.2773933020076242 2307 54-55 1.575542688341816 2307 56-57 1.2311748087685501 2307 58-59 1.2101116710214157 2307 60-61 1.1312472803557867 2307 62-63 1.0074695743717648 2307 64-65 1.0140706700308932 2307 66-67 1.0607330630493763 2307 68-69 0.8665205421052775 2307 70-71 0.7871458617720606 2307 72-73 0.7821856293445393 2307 74-75 0.9400222962043578 2307 76-77 0.9984237111750929 2307 78-79 0.9844432771848481 2307 80-81 1.4429574333102302 2307 82-83 1.2223538175878375 2307 84-85 1.06546705271154 2307 86-87 0.9967460292077455 2307 88-89 0.867628811965492 2307 90-91 0.61856193307419 2307 92-93 0.6920051225511052 2307 94-95 0.638945361827929 2307 96-97 0.7005485588916898 2307 98-99 0.5415098051681682 2307 100-101 0.5384655627626955 1101 1 1.0917930428035056 1101 2 0.5731066689035309 1101 3 0.383337214435695 1101 4 0.3461054256660461 1101 5 0.5518742222854485 1101 6 0.6099379634361455 1101 7 0.6915473323158281 1101 8 0.6651284198716425 1101 9 0.9453278788071984 1101 10-11 1.0402069613465983 1101 12-13 1.3325751721548258 1101 14-15 1.4005372362434088 1101 16-17 1.323672408833076 1101 18-19 0.9932395614124303 1101 20-21 1.2848696116280394 1101 22-23 0.9378708334929371 1101 24-25 1.1670322477603676 1101 26-27 1.1429203724767092 1101 28-29 1.4518783241060191 1101 30-31 1.3391372403636908 1101 32-33 0.8599810445772818 1101 34-35 0.9644359242156852 1101 36-37 1.0724959940487793 1101 38-39 0.906281902938801 1101 40-41 0.94622274206003 1101 42-43 1.049819663475013 1101 44-45 0.87691385666934 1101 46-47 0.7787789679559864 1101 48-49 0.8217093534123876 1101 50-51 0.8809180836959314 1101 52-53 1.0300206497605089 1101 54-55 1.4004429587471385 1101 56-57 1.1088754602947972 1101 58-59 1.0944461695193652 1101 60-61 0.9125033778868286 1101 62-63 0.6121461354455455 1101 64-65 0.65345167865026 1101 66-67 0.7395166485393894 1101 68-69 0.6551682035010202 1101 70-71 0.7455042314826237 1101 72-73 0.6438522265834656 1101 74-75 0.7723895642148371 1101 76-77 0.713987692842899 1101 78-79 0.6941553187968061 1101 80-81 1.2808733945383182 1101 82-83 1.111481497971326 1101 84-85 0.9785867357311702 1101 86-87 0.8430798515945703 1101 88-89 0.7681533548247543 1101 90-91 0.6086798818470527 1101 92-93 0.6628075870733241 1101 94-95 0.693766979441083 1101 96-97 0.651252280452006 1101 98-99 0.6988889200644799 1101 100-101 0.5970618555776568 2201 1 1.4733815996443766 2201 2 0.756536915091182 2201 3 0.35177595741855683 2201 4 0.22146559218513318 2201 5 0.41572218528085614 2201 6 0.5047244749521198 2201 7 0.6047800988707053 2201 8 0.5493775606344116 2201 9 0.5371795457236672 2201 10-11 0.8717046779211799 2201 12-13 1.1036669995206623 2201 14-15 1.0723541585148908 2201 16-17 0.930746172788723 2201 18-19 0.9276208846779994 2201 20-21 1.1673246030396882 2201 22-23 0.9129067924767185 2201 24-25 0.9075857881203078 2201 26-27 1.1467413821936034 2201 28-29 1.3772760079894724 2201 30-31 1.2791204187941716 2201 32-33 0.8387707248540934 2201 34-35 0.7804193943306714 2201 36-37 1.003729924981279 2201 38-39 0.7186264694513227 2201 40-41 1.0082937113019383 2201 42-43 1.0181185545410472 2201 44-45 0.9282923681263782 2201 46-47 0.9541887844983847 2201 48-49 0.9240817612031975 2201 50-51 0.939734603524343 2201 52-53 1.0031709056514302 2201 54-55 1.3572618152702383 2201 56-57 0.820171500427378 2201 58-59 1.0804000731402468 2201 60-61 0.9261738357009577 2201 62-63 0.6916572341730607 2201 64-65 0.7822761261028681 2201 66-67 0.7667848397403674 2201 68-69 0.7378675182319903 2201 70-71 0.7957007698284926 2201 72-73 0.7807704003194935 2201 74-75 0.6824944114883529 2201 76-77 0.6843559309329805 2201 78-79 0.7671646176886249 2201 80-81 1.2250531676059175 2201 82-83 1.0962891380166333 2201 84-85 1.0623214124153932 2201 86-87 1.103442528152474 2201 88-89 0.9107162995421376 2201 90-91 0.7904449407119927 2201 92-93 1.0094324315616063 2201 94-95 0.8779433349791681 2201 96-97 0.7759536087215579 2201 98-99 0.9291322395113255 2201 100-101 0.8569301776933358 2304 1 -1.6978835322240613 2304 2 -0.19667249217960148 2304 3 -0.1386346744452034 2304 4 0.12213749551593622 2304 5 0.46049519555133145 2304 6 0.6023384847451183 2304 7 0.6763722881895333 2304 8 0.7937234925452898 2304 9 0.9631032177549486 2304 10-11 0.9045824558029807 2304 12-13 1.1794921330119053 2304 14-15 1.2861578241797886 2304 16-17 1.1891539366582435 2304 18-19 0.9373382046793708 2304 20-21 1.1537991546620248 2304 22-23 0.9842703848657735 2304 24-25 1.1915538875357896 2304 26-27 1.2629758992377305 2304 28-29 1.7031491234301441 2304 30-31 1.5127051307308363 2304 32-33 1.0165937799077227 2304 34-35 1.0725323321367028 2304 36-37 1.3891353763548864 2304 38-39 1.1242580679446093 2304 40-41 1.2071543155468234 2304 42-43 1.069517754997932 2304 44-45 1.1266376540363794 2304 46-47 0.8805926661210606 2304 48-49 0.9700016570940662 2304 50-51 0.8301422706497661 2304 52-53 1.2237146698704748 2304 54-55 1.4506534135138587 2304 56-57 1.1456221406548224 2304 58-59 0.9132009238641103 2304 60-61 0.499396631105931 2304 62-63 0.35990535468499374 2304 64-65 0.4149715725768566 2304 66-67 0.4112147985773156 2304 68-69 0.599609286401936 2304 70-71 0.43617904453802936 2304 72-73 0.22046099086416504 2304 74-75 0.5087595745645679 2304 76-77 0.5762607909193136 2304 78-79 0.6305307503903101 2304 80-81 1.1061410237256055 2304 82-83 0.9678531623212443 2304 84-85 0.5085355975960688 2304 86-87 0.5858475863081587 2304 88-89 0.512032331286683 2304 90-91 0.3540526215688331 2304 92-93 0.24686311129808658 2304 94-95 0.11804930096893784 2304 96-97 0.06874570566538196 2304 98-99 0.5046648001402261 2304 100-101 0.6088876851910001 1102 1 1.3899325502083926 1102 2 0.125874299287549 1102 3 0.280300868246826 1102 4 0.21988055986416555 1102 5 0.32062935354277755 1102 6 0.23441594981542835 1102 7 -4.741606982310373 1102 8 -1.204238681417408 1102 9 0.2555190027511287 1102 10-11 0.807685632113234 1102 12-13 0.9341915534151042 1102 14-15 0.7572052034044816 1102 16-17 0.5036160887837937 1102 18-19 0.8397635324057191 1102 20-21 1.0766966014793553 1102 22-23 0.6767172032141318 1102 24-25 1.0864125930010857 1102 26-27 1.0823192173069316 1102 28-29 0.8187891127596743 1102 30-31 1.2259681826288116 1102 32-33 0.9261575219210272 1102 34-35 1.0879556364925875 1102 36-37 1.304790092942568 1102 38-39 0.9706102188829675 1102 40-41 1.1494105458485393 1102 42-43 1.2827983893864925 1102 44-45 0.9974375917441236 1102 46-47 0.950949361031121 1102 48-49 1.09312455962597 1102 50-51 0.7692178642869294 1102 52-53 0.9650746955091236 1102 54-55 1.36914093589181 1102 56-57 1.1567373759905593 1102 58-59 1.0869246195867603 1102 60-61 0.8706995664489057 1102 62-63 0.6093257988873937 1102 64-65 0.4687160689497425 1102 66-67 0.6914430213555747 1102 68-69 0.6746697076312493 1102 70-71 0.6558140576638962 1102 72-73 0.5453993342470262 1102 74-75 0.6377918086522065 1102 76-77 0.5540548229606586 1102 78-79 0.678849177394067 1102 80-81 1.2225408278269292 1102 82-83 1.0250187858968332 1102 84-85 0.8404442707567412 1102 86-87 0.6555545236105331 1102 88-89 0.5880936729568198 1102 90-91 0.1718260487766088 1102 92-93 0.28872597053707594 1102 94-95 0.34094235472666057 1102 96-97 0.16650020119553233 1102 98-99 0.10408699233950358 1102 100-101 0.44173468922687675 2202 1 1.4956811355287343 2202 2 0.917013210470806 2202 3 0.456067218659566 2202 4 -0.25136190874122377 2202 5 0.18266653778979247 2202 6 0.5334033823078244 2202 7 0.6395020901147959 2202 8 -4.107906911167127 2202 9 -0.3926520912317102 2202 10-11 0.5324097472974003 2202 12-13 0.02684738613902482 2202 14-15 0.321568886737694 2202 16-17 0.33891686727479 2202 18-19 0.9114718716924912 2202 20-21 1.2461720345239513 2202 22-23 -2.653041917104197 2202 24-25 -2.7962709094192846 2202 26-27 -1.4451249378244029 2202 28-29 0.6476376270163158 2202 30-31 1.4897064476360526 2202 32-33 1.2468324888117337 2202 34-35 -1.8731211188022172 2202 36-37 -0.15369089642052813 2202 38-39 0.45214070899815795 2202 40-41 1.3070176132799745 2202 42-43 1.399706869034766 2202 44-45 1.4558626053229773 2202 46-47 0.7060187651727858 2202 48-49 1.0907233206508522 2202 50-51 -3.79037621576299 2202 52-53 -0.21521797454522584 2202 54-55 1.5773901782889972 2202 56-57 1.4540924868656546 2202 58-59 1.6130653108332709 2202 60-61 1.5187956095662543 2202 62-63 1.3000940204351075 2202 64-65 1.3156086937903133 2202 66-67 1.4979999292088237 2202 68-69 1.3171565384672697 2202 70-71 1.444752559657502 2202 72-73 1.2784317995910506 2202 74-75 0.9219566257949232 2202 76-77 1.4076058173166004 2202 78-79 1.2965381249588042 2202 80-81 1.907481848064453 2202 82-83 1.9051114369260809 2202 84-85 1.6662867521232734 2202 86-87 1.609291093240106 2202 88-89 1.5187649431134425 2202 90-91 1.3450187625731438 2202 92-93 1.4113004434581526 2202 94-95 1.2250236413389466 2202 96-97 1.2831620653102078 2202 98-99 1.4448256683356178 2202 100-101 1.3560512013002324 2305 1 1.2710289639609265 2305 2 0.667702373787538 2305 3 0.12353109118631167 2305 4 -0.23428077393297286 2305 5 0.25779106320782574 2305 6 0.4465894362888676 2305 7 0.4873340148527703 2305 8 -0.09205495836202005 2305 9 0.5241536628276293 2305 10-11 0.3123338775662461 2305 12-13 0.8728383836501337 2305 14-15 0.6346692892729777 2305 16-17 0.6857032920348161 2305 18-19 0.7275388682882991 2305 20-21 0.8333602003308727 2305 22-23 0.46950814303873045 2305 24-25 0.7449320409797124 2305 26-27 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38-39 -0.3886005948501392 1208 40-41 -0.6792222591546349 1208 42-43 0.0036525377230347544 1208 44-45 0.12635964935831367 1208 46-47 -0.22402877347681738 1208 48-49 -1.5732589871876712 1208 50-51 -0.30867879199855963 1208 52-53 -1.780964279990588 1208 54-55 -1.0548811126151811 1208 56-57 -6.782304090389616 1208 58-59 -2.5180009012774747 1208 60-61 -1.6634968824035532 1208 62-63 0.30066978360511243 1208 64-65 -0.17937532546812207 1208 66-67 0.6269062121776088 1208 68-69 0.3922795753775681 1208 70-71 -4.330910150467524 1208 72-73 -0.821349497181906 1208 74-75 -5.870378728346083 1208 76-77 -1.3056465307593683 1208 78-79 -4.013557861721672 1208 80-81 0.720315904524309 1208 82-83 0.27808602786200964 1208 84-85 -2.7305886969951807 1208 86-87 -1.0236036290556534 1208 88-89 -5.127751954201241 1208 90-91 -0.2981745541757661 1208 92-93 -2.351456779198685 1208 94-95 -5.013382609117976 1208 96-97 -3.5027675000780505 1208 98-99 -6.139888250416572 1208 100-101 -3.8045506450647295 1209 1 1.7850452798593608 1209 2 1.1915747921621858 1209 3 0.6126306768683598 1209 4 0.25887026998577767 1209 5 0.3590591748771246 1209 6 0.8493740931597031 1209 7 0.7641840977949741 1209 8 0.36004558908275186 1209 9 0.21362747117216685 1209 10-11 -3.2636656874130168 1209 12-13 -0.6556390961646485 1209 14-15 -1.7090567157952776 1209 16-17 -0.6840237193464844 1209 18-19 -16.880960152915033 1209 20-21 -14.244040734177538 1209 22-23 -3.4369925343881533 1209 24-25 -2.7007539159741967 1209 26-27 -2.262161265524959 1209 28-29 -3.9635073387904427 1209 30-31 -1.1507679694989363 1209 32-33 -1.0164135638919731 1209 34-35 -3.3259969945909518 1209 36-37 -4.228741920283255 1209 38-39 -2.2786257935538394 1209 40-41 -10.894828918548274 1209 42-43 -5.528469588087454 1209 44-45 -3.2087469855510875 1209 46-47 -2.8245101096583483 1209 48-49 -3.2328308420257983 1209 50-51 -3.2097375549902623 1209 52-53 -1.1297010133427037 1209 54-55 -2.4046233055526365 1209 56-57 -1.2820391788820977 1209 58-59 -2.6162212578804755 1209 60-61 -2.656312161214885 1209 62-63 -1.733958966999964 1209 64-65 -0.8558782366850366 1209 66-67 -0.29821253641253875 1209 68-69 -1.297738237914409 1209 70-71 -1.6112463311981813 1209 72-73 -0.9276159387540694 1209 74-75 -0.025007575282621985 1209 76-77 -16.48387698611014 1209 78-79 -16.450720450758027 1209 80-81 -21.6548204646949 1209 82-83 -10.342179381358033 1209 84-85 -3.775384831534904 1209 86-87 -2.717148779208298 1209 88-89 -2.4160679920241854 1209 90-91 -5.70177165106675 1209 92-93 -3.340886408667764 1209 94-95 -0.7697691840449856 1209 96-97 -0.06568639139931065 1209 98-99 1.090613660558713 1209 100-101 0.46176168239158244 1212 1 -28.629416801269386 1212 2 -16.249922914273515 1212 3 -5.766222004126369 1212 4 -1.2025785627520449 1212 5 -0.45403173855200407 1212 6 -2.917797813809269 1212 7 -0.540451347764936 1212 8 -1.5704356518081042 1212 9 0.015867946467885474 1212 10-11 0.6684062352639444 1212 12-13 0.10594057015008218 1212 14-15 -0.2095796524324598 1212 16-17 0.023105915254028275 1212 18-19 -1.1004086237727435 1212 20-21 -1.165508774397935 1212 22-23 -0.8016165668062456 1212 24-25 0.3104802741401116 1212 26-27 -0.5493916698166146 1212 28-29 0.19848570595344484 1212 30-31 -0.63547157577338 1212 32-33 0.24804026557501402 1212 34-35 -1.26495458104948 1212 36-37 -1.655529796902023 1212 38-39 -0.31134910042220554 1212 40-41 -0.5119050863572525 1212 42-43 -0.030289924556420544 1212 44-45 0.8110401016918445 1212 46-47 -2.4729753999876003 1212 48-49 -1.5520806764954713 1212 50-51 -0.09172387910361124 1212 52-53 -1.132501607462352 1212 54-55 -0.4238009628362711 1212 56-57 -1.3300131473746717 1212 58-59 0.0019117191401356592 1212 60-61 0.4120881497633988 1212 62-63 -4.279251642506846 1212 64-65 -3.7068969689514226 1212 66-67 -1.4880435065892428 1212 68-69 0.21805922057376748 1212 70-71 -0.9685045127705294 1212 72-73 -1.1688478024962698 1212 74-75 0.07576311004273961 1212 76-77 1.0210670517162725 1212 78-79 -1.0925386795736252 1212 80-81 0.43257059845715773 1212 82-83 0.9028641536141002 1212 84-85 -2.8800019842542746 1212 86-87 -1.6809130481210097 1212 88-89 -0.679492849210785 1212 90-91 -0.05320793730198403 1212 92-93 -2.5380156257143014 1212 94-95 -2.1166024348617007 1212 96-97 -0.15867267773396065 1212 98-99 -0.9375622847536462 1212 100-101 -0.4325454663311987 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 2.0 11 12.0 12 61.0 13 266.0 14 832.0 15 2048.0 16 3635.0 17 6023.0 18 9254.0 19 13145.0 20 18068.0 21 24165.0 22 31527.0 23 39672.0 24 49342.0 25 60514.0 26 73270.0 27 87114.0 28 103048.0 29 121881.0 30 141838.0 31 165264.0 32 190277.0 33 215523.0 34 240392.0 35 260659.0 36 271122.0 37 242954.0 38 86018.0 39 253.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.244558035668554 17.622478769936798 15.468925743436351 20.664037450958297 2 22.89955750610326 27.811048556351963 27.585469016273866 21.703924921270907 3 20.087048282954516 26.085276237340203 30.432035785883514 23.39563969382176 4 23.0282473096977 26.419087935347317 27.879842859415156 22.672821895539833 5 21.9032602142559 27.69648463783398 27.341222090146296 23.059033057763827 6 21.494665569921185 27.745057119448436 27.50984263980454 23.250434670825836 7 22.647875543796186 26.648759123612493 28.562693893291875 22.14067143929945 8 21.97095251694138 26.931691794993213 28.452205735783597 22.64514995228181 9 22.509114459385025 26.572605283091328 27.996909911471164 22.921370346052488 10-11 23.101076323884886 27.05137373880372 26.841665914077968 23.00588402323343 12-13 22.739577737690674 27.206133490058875 26.96548179655079 23.08880697569966 14-15 22.440470730090517 27.369175140240863 27.31000538406135 22.88034874560727 16-17 22.908158066077082 27.144960620167524 26.961145354001516 22.985735959753875 18-19 22.89713098329516 27.143128272464544 27.002801245939455 22.956939498300844 20-21 22.681538633022292 27.23215774910076 27.204149245255234 22.88215437262172 22-23 22.72212202394331 27.314866701258634 26.978967989141566 22.98404328565649 24-25 22.743576350327192 26.861701045732172 27.274221355457 23.120501248483635 26-27 22.75016658652614 27.163428094421 27.05558416748638 23.03082115156648 28-29 23.076139195551836 27.200976168566854 27.00040110943779 22.722483526443526 30-31 22.954992754808227 26.918185878832407 26.957544233909452 23.169277132449917 32-33 22.714550834722573 27.26685819032114 27.04244437555885 22.97614659939744 34-35 23.06328416691685 27.079443263354786 26.879905556220002 22.977367013508356 36-37 23.12548460610321 26.928274635482648 26.903256146208864 23.04298461220528 38-39 22.70962849780854 27.200691405274302 27.321736144842003 22.76794395207515 40-41 23.057295975269103 27.016733590587517 26.923187231644096 23.002783202499288 42-43 22.83937885803411 27.024321226011743 27.099051250070172 23.037248665883975 44-45 22.807587070444743 26.90372397161805 27.281543840122495 23.00714511781471 46-47 23.068285854956645 27.003409816854585 27.07062959541994 22.857674732768828 48-49 23.03194923400677 26.85021973565006 27.163195061714333 22.954635968628835 50-51 22.931213336696253 26.924045431986265 27.042852842346775 23.1018883889707 52-53 23.052769785745742 26.98407661773814 27.108111598938645 22.855041997577473 54-55 22.73724986224231 26.791546630850682 27.315544539008563 23.15565896789844 56-57 22.792832007810752 27.043142200435287 27.15314362427029 23.010882167483675 58-59 23.008836044546694 26.85457173687318 27.31636704196103 22.820225176619093 60-61 23.00803431532781 26.712272900259894 27.092519212161537 23.187173572250767 62-63 22.878102310784847 27.02706001165512 27.2222679883937 22.872569689166333 64-65 22.97997457411645 27.01524535977625 27.025720823798626 22.97905924230867 66-67 23.015447628931447 27.020200119646255 26.960926202753004 23.003426048669297 68-69 22.811623930511253 27.200357028510613 27.11543257364425 22.872586467333885 70-71 23.158940903854038 26.9288506225957 26.858165832416635 23.054042641133627 72-73 22.877052644516084 27.05314304562481 27.084214662343907 22.985589647515198 74-75 22.750758465109588 26.98151744301871 27.282746472926117 22.98497761894558 76-77 23.060857037036737 27.0298819252632 27.01221899748726 22.897042040212803 78-79 23.055996036082067 26.994630974048217 26.949231421372122 23.000141568497593 80-81 22.878152736469872 26.973790451908776 27.12238691080445 23.025669900816904 82-83 23.040931723234785 27.04287389816966 26.943608987379932 22.972585391215624 84-85 22.877725880460964 26.72643333622179 27.138242003141507 23.25759878017574 86-87 22.8233328885316 27.04861710056533 27.136960420239998 22.99108959066307 88-89 22.99147889658932 26.95274438927346 26.957300911507865 23.098475802629356 90-91 23.079211079264788 26.818658255331545 26.851433654129416 23.250697011274248 92-93 22.803657480517582 27.026168248253658 27.081825029806687 23.088349241422073 94-95 23.043319407804262 26.852401868961152 26.89274223341183 23.211536489822755 96-97 23.065168361959344 26.875462659994664 26.765593943041992 23.293775035004 98-99 22.826826141005657 27.019232001516734 26.989145446219826 23.164796411257782 100-101 23.15613305461052 26.75242627990486 26.7084452523681 23.382995413116518 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 2.5 3 5.5 4 9.5 5 16.0 6 28.0 7 47.0 8 78.0 9 135.5 10 192.0 11 266.0 12 413.0 13 588.5 14 804.0 15 1141.0 16 1663.0 17 2412.0 18 3366.5 19 4709.0 20 6480.0 21 8595.0 22 11200.0 23 14250.0 24 17951.5 25 22125.0 26 26643.5 27 31349.0 28 36197.5 29 41346.5 30 46456.0 31 51218.5 32 55682.0 33 60106.0 34 64090.5 35 67726.5 36 71147.0 37 74032.5 38 75956.5 39 76122.0 40 75175.0 41 73568.5 42 70848.5 43 67587.5 44 64716.5 45 61893.0 46 58938.5 47 56744.0 48 54463.0 49 51452.5 50 48932.0 51 46889.0 52 44499.5 53 42584.0 54 41344.5 55 40324.0 56 39859.0 57 40211.0 58 40362.0 59 39978.0 60 39833.5 61 39846.0 62 39776.0 63 39330.5 64 38743.0 65 38016.0 66 37220.0 67 36181.0 68 34602.5 69 32505.0 70 29840.5 71 26456.5 72 22769.0 73 18896.0 74 14858.0 75 11226.5 76 8052.5 77 5464.5 78 3494.5 79 2168.0 80 1347.0 81 866.0 82 543.0 83 351.0 84 258.5 85 180.0 86 124.0 87 99.0 88 68.5 89 42.0 90 36.5 91 20.5 92 16.0 93 14.5 94 6.5 95 4.0 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7182950652148621 2 0.0028069524551070668 3 2.440828221832232E-4 4 4.068047036387053E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.1885946606069038 14-15 2.0340235181935265E-5 16-17 3.05103527729029E-4 18-19 0.11577661865557554 20-21 0.0 22-23 2.0340235181935265E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.001728919990464498 42-43 0.0 44-45 0.0 46-47 0.023269229048133944 48-49 4.068047036387053E-5 50-51 8.136094072774106E-5 52-53 1.830621166374174E-4 54-55 0.0025018489273780377 56-57 0.001301775051643857 58-59 0.0948668568885461 60-61 0.0015661981090090156 62-63 0.0014034762275535334 64-65 0.0023187868107406207 66-67 0.00416974821229673 68-69 0.004678254091845112 70-71 0.0034985204512928657 72-73 0.038870189432678295 74-75 0.043995928698525984 76-77 0.15820634924509253 78-79 3.2544376291096424E-4 80-81 0.11693601206094585 82-83 0.00459689315111737 84-85 0.0050036978547560755 86-87 0.006427514317491545 88-89 0.006793638550766379 90-91 0.022211536818673314 92-93 0.010597262529788275 94-95 0.014075442745899204 96-97 0.010109096885421828 98-99 0.011126108644518592 100-101 0.012366862990616644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2458182.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.14229894613572 #Duplication Level Percentage of deduplicated Percentage of total 1 98.22657616145361 95.41955425931293 2 1.286946418871792 2.5003386749940484 3 0.25780956397229493 0.7513264120370872 4 0.0968363627971911 0.3762762761480477 5 0.05948984367328251 0.2889490089184445 6 0.03238414303566981 0.18875220623312738 7 0.022242450081324815 0.1512477914566189 8 0.006497945355394319 0.05049802801994953 9 0.00289267593421482 0.02529010713202096 >10 0.008063470742443112 0.08888614447465162 >50 2.1904462639360425E-4 0.013831024076786875 >100 0.0 0.0 >500 0.0 0.0 >1k 4.191945638423878E-5 0.1450500671963198 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC 3562 0.14490383543610685 TruSeq Adapter, Index 7 (97% over 35bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.220414110916116E-4 0.0 4.068047036387054E-5 2 1.220414110916116E-4 0.0 4.068047036387054E-5 3 1.6272188145548215E-4 0.0 4.068047036387054E-5 4 1.6272188145548215E-4 0.0 4.068047036387054E-5 5 1.6272188145548215E-4 0.0 4.068047036387054E-5 6 1.6272188145548215E-4 0.0 4.068047036387054E-5 7 2.0340235181935266E-4 0.0 4.068047036387054E-5 8 2.440828221832232E-4 0.0 4.068047036387054E-5 9 2.440828221832232E-4 0.0 4.068047036387054E-5 10-11 2.440828221832232E-4 0.0 4.068047036387054E-5 12-13 2.440828221832232E-4 0.0 8.136094072774107E-5 14-15 2.8476329254709375E-4 0.0 8.136094072774107E-5 16-17 2.8476329254709375E-4 0.0 1.220414110916116E-4 18-19 3.05103527729029E-4 0.0 1.220414110916116E-4 20-21 3.254437629109643E-4 0.0 1.4238164627354688E-4 22-23 3.254437629109643E-4 0.0 1.6272188145548215E-4 24-25 3.254437629109643E-4 0.0 2.8476329254709375E-4 26-27 3.6612423327483484E-4 0.0 2.8476329254709375E-4 28-29 5.898668202761227E-4 2.034023518193527E-5 3.254437629109643E-4 30-31 9.559910535509576E-4 4.068047036387054E-5 3.6612423327483484E-4 32-33 0.0014238164627354687 4.068047036387054E-5 3.6612423327483484E-4 34-35 0.002013683283011591 8.136094072774107E-5 3.6612423327483484E-4 36-37 0.0029289938661986783 8.136094072774107E-5 3.864644684567701E-4 38-39 0.004088387271568988 8.136094072774107E-5 4.0680470363870533E-4 40-41 0.006142751024944451 8.136094072774107E-5 4.0680470363870533E-4 42-43 0.008441197600503136 8.136094072774107E-5 4.0680470363870533E-4 44-45 0.010841345351971498 8.136094072774107E-5 4.0680470363870533E-4 46-47 0.014665309566175328 8.136094072774107E-5 4.0680470363870533E-4 48-49 0.019669007420931403 8.136094072774107E-5 4.474851740025759E-4 50-51 0.026076181503241015 8.136094072774107E-5 4.474851740025759E-4 52-53 0.03372410993164868 8.136094072774107E-5 4.474851740025759E-4 54-55 0.04320265952643051 8.136094072774107E-5 4.474851740025759E-4 56-57 0.055366120165227795 8.136094072774107E-5 4.6782540918451114E-4 58-59 0.06887203632603281 8.136094072774107E-5 4.881656443664464E-4 60-61 0.08465605882721458 8.136094072774107E-5 5.28846114730317E-4 62-63 0.10310465213722987 8.136094072774107E-5 5.28846114730317E-4 64-65 0.12511278660408381 8.136094072774107E-5 5.28846114730317E-4 66-67 0.1529585685681532 8.136094072774107E-5 5.28846114730317E-4 68-69 0.18621485309061736 8.136094072774107E-5 5.28846114730317E-4 70-71 0.22225774983300667 8.136094072774107E-5 5.695265850941875E-4 72-73 0.26303992137278687 8.136094072774107E-5 5.695265850941875E-4 74-75 0.30742231453976965 8.136094072774107E-5 6.10207055458058E-4 76-77 0.36175108271071876 8.136094072774107E-5 6.10207055458058E-4 78-79 0.42407356330816837 1.220414110916116E-4 6.10207055458058E-4 80-81 0.49190824763992247 1.220414110916116E-4 6.10207055458058E-4 82-83 0.5682858307480894 1.220414110916116E-4 6.10207055458058E-4 84-85 0.6551386349749531 1.220414110916116E-4 6.10207055458058E-4 86-87 0.7514699887965985 1.220414110916116E-4 6.10207055458058E-4 88-89 0.8578087383277561 1.220414110916116E-4 6.10207055458058E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGTAT 895 0.0 23.60337 42-43 TCTCGTA 980 0.0 21.551094 40-41 CGTATGC 1015 0.0 21.046675 44-45 TATCTCG 1090 0.0 20.677637 38-39 TATGCCG 1100 0.0 19.636124 46-47 TCGTATG 1070 0.0 19.521173 42-43 CACGATA 1100 0.0 19.195576 34-35 ACGATAT 1190 0.0 18.142548 34-35 GTATGCC 1295 0.0 17.412489 44-45 CGATATC 1285 0.0 17.355156 36-37 GCCGTCT 1450 0.0 15.8711195 48-49 CCGTCTT 1525 0.0 15.557923 50-51 ATCTCGT 1445 0.0 14.944416 40-41 ATGCCGT 1615 0.0 14.550224 46-47 GAGCACA 3710 0.0 13.350973 9 GATATCT 1660 0.0 13.291643 36-37 AGTCACC 1835 0.0 12.928558 28-29 AGAGCAC 3760 0.0 12.920097 8 CCACGAT 1765 0.0 12.76924 32-33 ACCACGA 1840 0.0 12.764491 32-33 >>END_MODULE