FastQCFastQC Report
Thu 11 Sep 2014
LONLib31_CACGAT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib31_CACGAT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458182
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC35620.14490383543610685TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8950.023.6033742-43
TCTCGTA9800.021.55109440-41
CGTATGC10150.021.04667544-45
TATCTCG10900.020.67763738-39
TATGCCG11000.019.63612446-47
TCGTATG10700.019.52117342-43
CACGATA11000.019.19557634-35
ACGATAT11900.018.14254834-35
GTATGCC12950.017.41248944-45
CGATATC12850.017.35515636-37
GCCGTCT14500.015.871119548-49
CCGTCTT15250.015.55792350-51
ATCTCGT14450.014.94441640-41
ATGCCGT16150.014.55022446-47
GAGCACA37100.013.3509739
GATATCT16600.013.29164336-37
AGTCACC18350.012.92855828-29
AGAGCAC37600.012.9200978
CCACGAT17650.012.7692432-33
ACCACGA18400.012.76449132-33
Produced by FastQC (version 0.11.2)