##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	LONLib30_CACCGG_L007_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1762923
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.86355161286114	27.0	22.0	33.0	2.0	33.0
2	24.845652929821664	33.0	15.0	33.0	2.0	33.0
3	25.75974787327637	33.0	22.0	33.0	6.0	33.0
4	27.905892656684383	33.0	22.0	37.0	2.0	37.0
5	27.974276811863025	33.0	15.0	37.0	6.0	37.0
6	29.05690719333743	33.0	27.0	37.0	15.0	37.0
7	28.721008234619436	33.0	27.0	37.0	6.0	37.0
8	28.490371388880853	33.0	27.0	37.0	2.0	37.0
9	29.26672577304851	33.0	27.0	37.0	15.0	37.0
10-11	28.90090491757156	33.0	27.0	37.0	10.5	37.0
12-13	29.203067859458407	33.0	27.0	37.0	10.5	37.0
14-15	29.56308840488212	33.0	27.0	37.0	6.0	40.0
16-17	30.450989351208193	33.0	27.0	37.0	15.0	40.0
18-19	30.784557805417478	33.0	27.0	37.0	15.0	40.0
20-21	30.812124806358533	33.0	27.0	37.0	15.0	40.0
22-23	30.84987914957148	33.0	27.0	37.0	15.0	40.0
24-25	30.462826510290014	33.0	27.0	37.0	10.5	40.0
26-27	29.95358390582005	33.0	27.0	37.0	15.0	40.0
28-29	30.173254872731256	33.0	27.0	37.0	15.0	40.0
30-31	30.1111353133404	33.0	27.0	37.0	15.0	40.0
32-33	29.968320510878804	33.0	27.0	37.0	15.0	40.0
34-35	30.008351187204433	33.0	27.0	37.0	15.0	40.0
36-37	30.23249966107425	33.0	27.0	37.0	15.0	40.0
38-39	30.288657814323145	33.0	27.0	37.0	15.0	40.0
40-41	30.39800291901575	33.0	27.0	37.0	15.0	40.0
42-43	30.34769981445588	33.0	27.0	37.0	15.0	40.0
44-45	30.108764534809517	33.0	27.0	37.0	15.0	40.0
46-47	29.76085824508501	33.0	27.0	37.0	15.0	40.0
48-49	29.619690423234594	33.0	27.0	37.0	10.5	40.0
50-51	28.885682188047916	33.0	24.5	37.0	15.0	38.5
52-53	28.62407149943588	33.0	22.0	37.0	10.5	38.5
54-55	29.58854158689858	33.0	27.0	37.0	15.0	40.0
56-57	29.53098291870944	33.0	27.0	37.0	15.0	40.0
58-59	29.745934734528962	33.0	27.0	37.0	15.0	40.0
60-61	29.512541954469935	33.0	27.0	37.0	10.5	40.0
62-63	29.401675512770552	33.0	27.0	37.0	15.0	40.0
64-65	29.37033381491988	33.0	27.0	37.0	15.0	40.0
66-67	29.258815614748915	33.0	27.0	37.0	15.0	40.0
68-69	28.989004057465927	33.0	27.0	37.0	15.0	40.0
70-71	28.908818195689772	33.0	27.0	37.0	15.0	40.0
72-73	28.672266457468645	33.0	24.5	37.0	15.0	37.0
74-75	28.62495837878342	33.0	27.0	37.0	15.0	37.0
76-77	28.239389071445547	33.0	24.5	37.0	15.0	37.0
78-79	27.678014014225237	33.0	22.0	37.0	10.5	37.0
80-81	27.54656697995318	33.0	22.0	35.0	10.5	37.0
82-83	27.126021386073013	27.0	22.0	33.0	6.0	37.0
84-85	27.225105974566105	27.0	22.0	33.0	15.0	37.0
86-87	26.916606113823462	27.0	22.0	33.0	6.0	37.0
88-89	26.560812639009193	27.0	22.0	33.0	6.0	37.0
90-91	26.445723097378615	27.0	22.0	33.0	6.0	37.0
92-93	26.33053740861059	27.0	22.0	33.0	6.0	37.0
94-95	26.121011808229852	27.0	22.0	33.0	6.0	37.0
96-97	25.996957325986443	27.0	22.0	33.0	6.0	37.0
98-99	25.779890273142957	27.0	22.0	33.0	6.0	37.0
100-101	25.318447827840465	27.0	18.5	33.0	6.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2306	1	8.37675976469891
2306	2	8.580326566990394
2306	3	7.513615257552512
2306	4	9.049694574280192
2306	5	8.997086651644857
2306	6	7.960612406233341
2306	7	8.29250956829118
2306	8	8.572057022694548
2306	9	7.664928771380552
2306	10-11	7.95525071652153
2306	12-13	7.512570238883509
2306	14-15	8.178070860090624
2306	16-17	6.892089961511065
2306	18-19	6.336196185895766
2306	20-21	6.201285550326006
2306	22-23	5.9332040889382185
2306	24-25	6.024017824105552
2306	26-27	6.6232021035876265
2306	28-29	5.695776759247508
2306	30-31	5.667396868690037
2306	32-33	5.591256528237864
2306	34-35	5.039147439867129
2306	36-37	5.112355958832023
2306	38-39	4.766379641871541
2306	40-41	3.9845591471117032
2306	42-43	3.894518395185507
2306	44-45	3.5544173051470835
2306	46-47	3.978205227129532
2306	48-49	3.740662743305581
2306	50-51	3.593028982907473
2306	52-53	4.243768238973285
2306	54-55	3.6541843560911786
2306	56-57	3.968686749260833
2306	58-59	3.52461527990825
2306	60-61	3.6444198076526284
2306	62-63	2.9709007830534766
2306	64-65	2.7320911847206766
2306	66-67	2.63347322317556
2306	68-69	2.755923876816535
2306	70-71	2.8600011596838364
2306	72-73	2.067234249945791
2306	74-75	1.6825026413962334
2306	76-77	2.165384139685372
2306	78-79	2.2841520454049657
2306	80-81	2.0108315279281186
2306	82-83	2.057262404284181
2306	84-85	1.611518671430833
2306	86-87	1.6350948763428548
2306	88-89	1.580619071005195
2306	90-91	1.5490193315033771
2306	92-93	1.429464850202617
2306	94-95	1.6696221650937204
2306	96-97	1.4854945078498503
2306	98-99	1.3448675925310596
2306	100-101	1.2567683678015058
2307	1	8.414766439309268
2307	2	8.558920069851279
2307	3	7.650116564032803
2307	4	9.148552810767104
2307	5	9.156360888371857
2307	6	7.694887342068828
2307	7	8.270238495626863
2307	8	8.673109982378836
2307	9	7.549214040380441
2307	10-11	7.862289374833448
2307	12-13	7.332280698842329
2307	14-15	8.248406288930585
2307	16-17	7.022559666923801
2307	18-19	6.411182527703197
2307	20-21	6.502717972453844
2307	22-23	6.179484900013193
2307	24-25	6.263826595768538
2307	26-27	7.101637369211343
2307	28-29	6.212205433471823
2307	30-31	6.2381760097483046
2307	32-33	6.286646709208064
2307	34-35	5.914905461028631
2307	36-37	5.878010191371185
2307	38-39	5.426376620358777
2307	40-41	4.818695011513306
2307	42-43	4.628828661576534
2307	44-45	4.47357021756482
2307	46-47	4.8665665373065785
2307	48-49	4.718512974488821
2307	50-51	4.4137696559477355
2307	52-53	5.10016296721788
2307	54-55	4.694880347573651
2307	56-57	5.019418903408457
2307	58-59	4.635495283564072
2307	60-61	4.5265689080534735
2307	62-63	4.287907978073367
2307	64-65	4.149641604083456
2307	66-67	3.7024411617194914
2307	68-69	3.8803069050543826
2307	70-71	3.6631793068485585
2307	72-73	3.2158417963614774
2307	74-75	2.8253270942330104
2307	76-77	2.886475696978504
2307	78-79	3.2356739193158077
2307	80-81	3.1113963052740026
2307	82-83	3.3069156805793583
2307	84-85	2.420754065008449
2307	86-87	2.4762343754265146
2307	88-89	2.7363936388708936
2307	90-91	2.360788294224186
2307	92-93	2.2664786543553923
2307	94-95	2.490238055795441
2307	96-97	2.376680079887695
2307	98-99	2.1472632156345135
2307	100-101	1.8877579371029327
1101	1	8.08028361046036
1101	2	7.875406074420329
1101	3	7.359972603203296
1101	4	8.974800538006317
1101	5	8.683850531465012
1101	6	7.488364966304307
1101	7	7.2051013996059226
1101	8	8.152466537375613
1101	9	7.47747977553999
1101	10-11	7.574985829502186
1101	12-13	7.281044050104928
1101	14-15	7.855259931211908
1101	16-17	6.978969256165971
1101	18-19	6.136787631609366
1101	20-21	5.870232328857853
1101	22-23	5.406979704880577
1101	24-25	6.024130971899655
1101	26-27	6.6056144635748915
1101	28-29	5.9989192724343
1101	30-31	5.787207141216232
1101	32-33	5.912892680741763
1101	34-35	5.606182027486653
1101	36-37	5.500646748390533
1101	38-39	5.127450796107706
1101	40-41	4.734112563933401
1101	42-43	4.465296561310296
1101	44-45	4.4938230532048
1101	46-47	4.8193171309106155
1101	48-49	4.449230482173551
1101	50-51	4.057599018226334
1101	52-53	5.061624458626508
1101	54-55	4.598923912948045
1101	56-57	4.808867464397103
1101	58-59	4.3534832239740275
1101	60-61	4.523723857492666
1101	62-63	4.085617941140345
1101	64-65	3.7804666662881026
1101	66-67	3.3980645741227775
1101	68-69	3.5665934755224313
1101	70-71	3.5967390910793746
1101	72-73	3.1171183347282074
1101	74-75	2.4537601130141447
1101	76-77	2.7559048258184973
1101	78-79	3.25794543004422
1101	80-81	2.8940343089946943
1101	82-83	3.087882498447385
1101	84-85	2.261047225692721
1101	86-87	2.360235192883003
1101	88-89	2.496671679419265
1101	90-91	2.3553456071239154
1101	92-93	2.1075189028509236
1101	94-95	2.2791714576715165
1101	96-97	2.211589194035941
1101	98-99	2.0325438370699906
1101	100-101	1.7916901205193838
2201	1	7.066726660626049
2201	2	7.932084954498567
2201	3	6.4659332393589
2201	4	8.347850029723052
2201	5	3.750948289358117
2201	6	5.66851163222351
2201	7	6.671080739057089
2201	8	7.702069848203262
2201	9	6.604830363860259
2201	10-11	4.126501776959113
2201	12-13	6.395598900962515
2201	14-15	6.992661441290959
2201	16-17	5.926043947781
2201	18-19	2.0023020551718247
2201	20-21	4.985869002014404
2201	22-23	5.119204790266323
2201	24-25	5.445964927871319
2201	26-27	6.160104923697379
2201	28-29	5.138905188273249
2201	30-31	5.232661776381246
2201	32-33	5.332426832627718
2201	34-35	4.873474649867994
2201	36-37	4.875350835085808
2201	38-39	4.453809236326766
2201	40-41	4.29930062464647
2201	42-43	4.126993774103539
2201	44-45	3.931688660325989
2201	46-47	4.373062020730611
2201	48-49	4.187904626799071
2201	50-51	3.8083607030094555
2201	52-53	4.539184178261845
2201	54-55	4.200901385429027
2201	56-57	4.425510583975786
2201	58-59	3.955967541487457
2201	60-61	4.044234599394954
2201	62-63	3.8622200092108514
2201	64-65	3.696095339943522
2201	66-67	3.3510875562066254
2201	68-69	3.451843361251342
2201	70-71	3.2308964990058513
2201	72-73	2.5945313968427577
2201	74-75	2.252961611629349
2201	76-77	2.657739609035165
2201	78-79	3.061456686140165
2201	80-81	2.450575664840848
2201	82-83	2.786136696558284
2201	84-85	2.258703329868485
2201	86-87	2.0492989929987715
2201	88-89	2.1998358075565747
2201	90-91	2.177326892490477
2201	92-93	2.0418238921250875
2201	94-95	2.134111737511283
2201	96-97	2.05957998600125
2201	98-99	2.051068351131793
2201	100-101	1.5764582935068105
2304	1	1.297939768692995
2304	2	4.463555022972145
2304	3	5.628221381206412
2304	4	4.20907020923304
2304	5	6.502861671560893
2304	6	6.27794442734891
2304	7	7.173433637610735
2304	8	6.577015604733614
2304	9	5.729555322297081
2304	10-11	6.801983594494413
2304	12-13	6.4191296308776735
2304	14-15	6.664323203569712
2304	16-17	5.439088546178045
2304	18-19	5.094646916164962
2304	20-21	4.947175058631565
2304	22-23	4.465826666654273
2304	24-25	4.705922445539866
2304	26-27	5.5275010090431245
2304	28-29	4.688550207199761
2304	30-31	4.732818233735873
2304	32-33	4.751510470220964
2304	34-35	4.366413941462376
2304	36-37	4.52255479724084
2304	38-39	4.186971888909593
2304	40-41	3.604812593097371
2304	42-43	3.20236046264203
2304	44-45	2.9889005258585435
2304	46-47	3.905639367154837
2304	48-49	3.3501145399692582
2304	50-51	3.118002018485715
2304	52-53	3.8961918172988916
2304	54-55	3.472200853661878
2304	56-57	4.1463475905681975
2304	58-59	3.3290670743965016
2304	60-61	3.486457286784841
2304	62-63	3.2296685601017607
2304	64-65	3.159218502475337
2304	66-67	2.9619650994772826
2304	68-69	3.1223147863148455
2304	70-71	3.1378917261172496
2304	72-73	2.6738543363624636
2304	74-75	2.0118109120663803
2304	76-77	2.3546138165169417
2304	78-79	2.6225538198575293
2304	80-81	2.6660326048516616
2304	82-83	2.645107030043242
2304	84-85	2.237958641166287
2304	86-87	2.0085313858436002
2304	88-89	2.3086595461359245
2304	90-91	2.2683531373395276
2304	92-93	2.105427161359213
2304	94-95	2.4804392164412015
2304	96-97	2.305316523087413
2304	98-99	2.058358244067197
2304	100-101	1.647667859143084
1102	1	7.028650201504888
1102	2	7.477530032345772
1102	3	6.077637743450897
1102	4	6.0639545136643065
1102	5	6.594265737823747
1102	6	5.809778080874327
1102	7	6.819917353231716
1102	8	6.272492695986589
1102	9	4.771876591277103
1102	10-11	5.915915962053727
1102	12-13	5.807832779761188
1102	14-15	6.1330746764785395
1102	16-17	5.365079766506032
1102	18-19	5.378351838847031
1102	20-21	5.5015923721165265
1102	22-23	5.249790165261224
1102	24-25	2.076010348233769
1102	26-27	5.57607563701222
1102	28-29	5.1390778807279744
1102	30-31	5.070054781757374
1102	32-33	4.909093925802573
1102	34-35	4.990453428287061
1102	36-37	4.900370506888944
1102	38-39	3.987360963925372
1102	40-41	3.9752335561160663
1102	42-43	4.298677824723512
1102	44-45	-3.736514500107546
1102	46-47	1.5290112698830534
1102	48-49	-0.6761506981713943
1102	50-51	2.7441117780111135
1102	52-53	-0.1016606500977808
1102	54-55	2.6226315359864145
1102	56-57	-0.5773851779755681
1102	58-59	2.8129709753907868
1102	60-61	3.873655961810311
1102	62-63	3.621431423169053
1102	64-65	3.1186181965644764
1102	66-67	3.0519944340177076
1102	68-69	3.4319014231271936
1102	70-71	3.380892135377742
1102	72-73	2.8671067865005604
1102	74-75	2.4958711495585817
1102	76-77	2.7933947551717715
1102	78-79	-1.0630917847806884
1102	80-81	1.748101192065274
1102	82-83	2.525344088252602
1102	84-85	2.3478645112535865
1102	86-87	2.4156172947172863
1102	88-89	2.1793122888624694
1102	90-91	2.0803478003001423
1102	92-93	2.0861088046384815
1102	94-95	2.388358785400584
1102	96-97	2.401993489392307
1102	98-99	2.2276838927904876
1102	100-101	1.8918555779977808
2202	1	-20.634039926738478
2202	2	-7.704023201822091
2202	3	-0.6834144990840123
2202	4	1.4374284789757539
2202	5	1.0455149647590112
2202	6	1.5106627736595435
2202	7	3.9248415172939453
2202	8	2.473852920117171
2202	9	3.085300693233112
2202	10-11	2.015309300655762
2202	12-13	1.3995624173501824
2202	14-15	2.7225064926580416
2202	16-17	1.4717067395749908
2202	18-19	1.5741203821222314
2202	20-21	1.140022733639995
2202	22-23	1.2477187977083446
2202	24-25	2.199776462949071
2202	26-27	3.4738845817437713
2202	28-29	-0.9642496313707767
2202	30-31	0.8648082961972356
2202	32-33	1.3593359116527957
2202	34-35	1.5674408327973879
2202	36-37	2.847124102686866
2202	38-39	2.294879155827285
2202	40-41	1.8758485926086337
2202	42-43	0.6327488189864674
2202	44-45	0.6567097873187393
2202	46-47	-1.41196236858217
2202	48-49	0.6982312579156975
2202	50-51	1.646976941380931
2202	52-53	1.5614594903079606
2202	54-55	-0.8406035389967812
2202	56-57	1.1342653726162055
2202	58-59	1.9443166051032748
2202	60-61	1.909247720978268
2202	62-63	-2.346646849377411
2202	64-65	-0.1696662642850022
2202	66-67	0.3942991251801118
2202	68-69	1.2543925982791393
2202	70-71	-2.709064810109961
2202	72-73	-4.578541188042632
2202	74-75	-1.365960509734741
2202	76-77	-0.34933583674293445
2202	78-79	1.3964094663714306
2202	80-81	1.4554671203966514
2202	82-83	-0.934909779355209
2202	84-85	-0.49267259987826506
2202	86-87	0.2883382628869242
2202	88-89	0.9853452066823394
2202	90-91	1.1351514282606026
2202	92-93	0.9871428677797347
2202	94-95	1.5188720177237514
2202	96-97	0.9507463290388287
2202	98-99	1.0266190267039015
2202	100-101	0.7627131963862723
2305	1	5.505567105319432
2305	2	6.862043204012462
2305	3	6.555778656222728
2305	4	7.758340984057757
2305	5	7.7857810919603025
2305	6	6.21458136507562
2305	7	2.7570569987132423
2305	8	5.833679419241822
2305	9	6.185848531185314
2305	10-11	4.574232617673886
2305	12-13	5.137057109062162
2305	14-15	5.560421170838136
2305	16-17	5.005029399760833
2305	18-19	4.3868064550601815
2305	20-21	5.0104543059417
2305	22-23	4.837267636598128
2305	24-25	4.936234765639529
2305	26-27	5.835858533229601
2305	28-29	4.92593028093593
2305	30-31	5.41160797366452
2305	32-33	5.29542846104156
2305	34-35	5.000864341027025
2305	36-37	4.529494816173578
2305	38-39	4.569663486590766
2305	40-41	3.8930097314900465
2305	42-43	3.7447584104877762
2305	44-45	2.899181069763408
2305	46-47	3.6170523754470914
2305	48-49	3.188621653742384
2305	50-51	2.2871087679524678
2305	52-53	3.672162469813255
2305	54-55	3.947771329097442
2305	56-57	4.428965872028989
2305	58-59	4.238890573032538
2305	60-61	4.414812466252098
2305	62-63	3.5763793914797013
2305	64-65	3.779709073729432
2305	66-67	3.261129924489488
2305	68-69	3.741723987063054
2305	70-71	3.3850702764858447
2305	72-73	-1.332322423970485
2305	74-75	1.7888606603590276
2305	76-77	2.8486025863617535
2305	78-79	3.6754807818538318
2305	80-81	2.9945828859554986
2305	82-83	3.229260577655918
2305	84-85	2.6037138419809907
2305	86-87	2.5653920517788578
2305	88-89	2.6767113469327803
2305	90-91	2.5795580955457815
2305	92-93	2.6156322892951387
2305	94-95	2.673550816327115
2305	96-97	2.706046885626499
2305	98-99	2.691825961308055
2305	100-101	2.2613254986407583
1103	1	-20.634039926738478
1103	2	-7.7383602541935765
1103	3	-0.8055113933149087
1103	4	2.6276558100512233
1103	5	4.644090406495803
1103	6	4.337334982983343
1103	7	4.047271315381732
1103	8	4.255087395974424
1103	9	4.101666103767286
1103	10-11	4.819503014932845
1103	12-13	4.457477951706576
1103	14-15	4.113531481510467
1103	16-17	2.8166999874422487
1103	18-19	3.0299435899058977
1103	20-21	2.245131264536461
1103	22-23	2.5165669450646035
1103	24-25	2.9602610135871537
1103	26-27	3.9600333702943153
1103	28-29	3.656848749998481
1103	30-31	3.451173598103807
1103	32-33	2.8278919964808864
1103	34-35	2.592256477185593
1103	36-37	2.699054945056826
1103	38-39	2.2570842352009457
1103	40-41	2.576043300960748
1103	42-43	2.293037853189059
1103	44-45	2.602111832644006
1103	46-47	1.899198216660956
1103	48-49	-1.2352344686188168
1103	50-51	-1.8386116864219595
1103	52-53	1.7725445793680201
1103	54-55	2.2449151299812655
1103	56-57	3.1882001027945144
1103	58-59	2.8982871161537496
1103	60-61	3.1781403791566127
1103	62-63	2.826453485699002
1103	64-65	2.838401587164366
1103	66-67	2.7068305377654944
1103	68-69	2.997521836245916
1103	70-71	2.90005101950155
1103	72-73	2.3462683096129986
1103	74-75	2.0641020597637407
1103	76-77	2.3571054223516477
1103	78-79	2.8107075214339687
1103	80-81	2.4358400998901466
1103	82-83	2.5305797615289087
1103	84-85	1.9807327138130795
1103	86-87	1.7412234252308743
1103	88-89	2.0358668443732597
1103	90-91	-2.7345882470170544
1103	92-93	-2.97288992450866
1103	94-95	-3.0899904578780912
1103	96-97	0.19306721868298737
1103	98-99	-1.3196243658897622
1103	100-101	0.9039512675521877
2203	1	2.517244182410799
2203	2	3.218984105002491
2203	3	-5.852311790255296
2203	4	-3.289806331059687
2203	5	0.049982067657506235
2203	6	0.3491952387440236
2203	7	2.399804725599914
2203	8	-7.6023410098679705
2203	9	-6.814250371999119
2203	10-11	-12.207852958617678
2203	12-13	-10.037467588515415
2203	14-15	-1.3729060484801643
2203	16-17	-1.4558469903476343
2203	18-19	-0.40148602226678065
2203	20-21	-1.3374851603296847
2203	22-23	-3.1478647940741986
2203	24-25	-0.8562023775827932
2203	26-27	-1.6063687277525283
2203	28-29	0.4683725422251399
2203	30-31	-2.2450311550357718
2203	32-33	-0.1883339184556334
2203	34-35	-0.3283663775550991
2203	36-37	-0.5382874784135012
2203	38-39	0.31913328252652207
2203	40-41	-1.1081003357198576
2203	42-43	0.03523941471335945
2203	44-45	-0.26330545035501984
2203	46-47	0.047096700672852876
2203	48-49	0.23136588097791488
2203	50-51	0.25914946018069784
2203	52-53	0.8454113163116368
2203	54-55	-0.31040595852629593
2203	56-57	-0.010272714543525296
2203	58-59	0.3677186104115826
2203	60-61	0.14575465256854514
2203	62-63	0.4802791094603833
2203	64-65	0.07076862700241904
2203	66-67	-0.7343213589833617
2203	68-69	0.39642170849967684
2203	70-71	0.4997317122115028
2203	72-73	-0.10267794426209775
2203	74-75	-0.45834937072009296
2203	76-77	-0.29035424371233987
2203	78-79	0.42028089505993904
2203	80-81	0.2624602935909941
2203	82-83	-0.21360803243757687
2203	84-85	-0.30766994221253796
2203	86-87	-0.20088955643395678
2203	88-89	0.020493421304657033
2203	90-91	-0.6188675594146211
2203	92-93	-0.28596492105409155
2203	94-95	0.04072736694922341
2203	96-97	-0.04173332576902666
2203	98-99	-0.2686890517803633
2203	100-101	-0.6521453502335177
2310	1	-20.634039926738478
2310	2	-20.388560036249515
2310	3	-21.33184630068868
2310	4	-23.317494934590986
2310	5	-23.511013116933185
2310	6	-24.595828838302527
2310	7	-24.161591743099926
2310	8	-23.822645985790924
2310	9	-24.727974288532025
2310	10-11	-24.444209298906603
2310	12-13	-24.742122484181547
2310	14-15	-25.09642129727631
2310	16-17	-26.1404376805563
2310	18-19	-20.2890349789281
2310	20-21	-20.417178579270292
2310	22-23	-26.78510395940325
2310	24-25	-26.42622645462934
2310	26-27	-19.31715042919715
2310	28-29	-19.785627457774858
2310	30-31	-20.333312053912174
2310	32-33	-18.579157888493086
2310	34-35	-10.925486227742365
2310	36-37	-15.447426590238006
2310	38-39	-20.323885979110717
2310	40-41	-12.532815156479622
2310	42-43	-5.963610772552556
2310	44-45	-14.899244990215907
2310	46-47	-19.98786317091623
2310	48-49	-20.395054664768473
2310	50-51	-12.181825392681796
2310	52-53	-15.738972311296708
2310	54-55	-19.806613021126616
2310	56-57	-19.289645641242295
2310	58-59	-11.868487917143263
2310	60-61	-8.956586706446974
2310	62-63	-5.96311147373919
2310	64-65	-16.518987927304696
2310	66-67	-12.155945222011718
2310	68-69	-6.8656869057325345
2310	70-71	-4.532681877943663
2310	72-73	-5.072279335380344
2310	74-75	-5.144695010099436
2310	76-77	-4.103264356071893
2310	78-79	-4.790207601462267
2310	80-81	-4.276974163337844
2310	82-83	-13.92284559263019
2310	84-85	-11.735779091490228
2310	86-87	-7.453705500789226
2310	88-89	-5.421678863339537
2310	90-91	-3.14245396925946
2310	92-93	-2.5358520264580484
2310	94-95	-3.1986487700224195
2310	96-97	-2.0152164718364425
2310	98-99	-2.302750046964956
2310	100-101	-2.337310144241009
2204	1	-20.634039926738478
2204	2	-20.388560036249515
2204	3	-21.33184630068868
2204	4	-23.317494934590986
2204	5	-23.511013116933185
2204	6	-24.595828838302527
2204	7	-24.161591743099926
2204	8	-23.822645985790924
2204	9	-24.727974288532025
2204	10-11	-18.418468819357944
2204	12-13	-18.98930160971046
2204	14-15	-18.909185754257976
2204	16-17	-20.25750397110637
2204	18-19	-26.5730349789281
2204	20-21	-14.169129213967048
2204	22-23	-6.693072929784073
2204	24-25	-15.953729980722429
2204	26-27	-25.53815042919715
2204	28-29	-13.355499107986425
2204	30-31	-15.832723901584954
2204	32-33	-19.5943318424188
2204	34-35	-19.90437151218054
2204	36-37	-26.165093256904672
2204	38-39	-13.82123012579619
2204	40-41	-7.771068094890531
2204	42-43	-5.574620175467459
2204	44-45	-14.730454205072514
2204	46-47	-20.908187571480408
2204	48-49	-19.2755835787318
2204	50-51	-11.32869610260187
2204	52-53	-3.6239962887297104
2204	54-55	-25.937613021126616
2204	56-57	-19.768708170626404
2204	58-59	-12.309421156447446
2204	60-61	-16.401799212323795
2204	62-63	-13.00925580848296
2204	64-65	-6.585494744418
2204	66-67	-4.5409969380436905
2204	68-69	-8.111280229662952
2204	70-71	-8.053028375357858
2204	72-73	-4.474439655079447
2204	74-75	-4.132534690400334
2204	76-77	-4.234937134633245
2204	78-79	-18.523600173159494
2204	80-81	-8.242254370201973
2204	82-83	-4.994298342982802
2204	84-85	-3.560573167653839
2204	86-87	-3.130031781936381
2204	88-89	-4.290809093710955
2204	90-91	-3.6316928032980087
2204	92-93	-3.212456633886351
2204	94-95	-2.525481398137135
2204	96-97	-2.5776776848124676
2204	98-99	-2.8635610483753915
2204	100-101	-2.991313905406969
2311	1	8.307874095779436
2311	2	8.565892369072902
2311	3	7.5719408026890065
2311	4	9.092433417507277
2311	5	8.78146385338411
2311	6	7.954580578278847
2311	7	8.278786967237842
2311	8	8.231089940514092
2311	9	7.702680777998168
2311	10-11	7.920299401195745
2311	12-13	7.504295120731452
2311	14-15	8.186587914596767
2311	16-17	7.007847887509204
2311	18-19	6.2415760753196
2311	20-21	6.281670960136054
2311	22-23	6.008524495049151
2311	24-25	6.063282245473015
2311	26-27	6.68933165882741
2311	28-29	5.590429860546536
2311	30-31	5.471755499823747
2311	32-33	5.7975650729535175
2311	34-35	5.398979142438456
2311	36-37	5.374057203688981
2311	38-39	4.6975817793335
2311	40-41	3.908069524516627
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2311	44-45	3.6017799159594617
2311	46-47	3.9439126735054693
2311	48-49	3.7969770650165806
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2311	52-53	4.200131066390089
2311	54-55	3.675616252158953
2311	56-57	3.9143639118112716
2311	58-59	3.380413481696724
2311	60-61	3.4004753884012047
2311	62-63	2.9040679871956456
2311	64-65	2.856522137519594
2311	66-67	2.4443993702776012
2311	68-69	2.6025215555434826
2311	70-71	2.382404781899396
2311	72-73	1.7294316847493043
2311	74-75	1.5996226289316091
2311	76-77	1.7294193696189986
2311	78-79	1.9112840396158646
2311	80-81	1.7517777984499396
2311	82-83	1.8628708863872063
2311	84-85	1.3451451664422187
2311	86-87	1.2992242160309715
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2311	90-91	0.8311103432618658
2311	92-93	0.8479797715631037
2311	94-95	1.232912471874542
2311	96-97	1.1653311228411418
2311	98-99	0.8238159714587887
2311	100-101	0.7395073241997991
2205	1	8.41779701337224
2205	2	8.562874286850636
2205	3	7.531012280086355
2205	4	9.289701844472926
2205	5	9.217723672196158
2205	6	7.785399143579706
2205	7	8.336143536215623
2205	8	8.534173339825582
2205	9	7.6633191186144245
2205	10-11	7.896756981918767
2205	12-13	7.562859901324234
2205	14-15	8.159240504434809
2205	16-17	7.039607613857388
2205	18-19	6.421177401544973
2205	20-21	6.506088154499203
2205	22-23	6.098766196610839
2205	24-25	6.002686479442122
2205	26-27	6.639001055453075
2205	28-29	6.051097756115425
2205	30-31	5.899667815237297
2205	32-33	6.015025134489633
2205	34-35	5.796820935485279
2205	36-37	5.769733114509517
2205	38-39	5.0000792951721245
2205	40-41	4.657110694887599
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2205	44-45	4.441054288428617
2205	46-47	4.780667276995192
2205	48-49	4.41977422300204
2205	50-51	4.252926830099614
2205	52-53	4.9658711713404315
2205	54-55	4.631209324117471
2205	56-57	4.711648101418334
2205	58-59	4.267152914931895
2205	60-61	4.346788398401213
2205	62-63	3.9102894657005045
2205	64-65	3.729077833473699
2205	66-67	3.6023226860573985
2205	68-69	3.3844873938816242
2205	70-71	3.3945684156312446
2205	72-73	2.7799291866797127
2205	74-75	2.3932927436331575
2205	76-77	2.569454315291974
2205	78-79	3.036445980151555
2205	80-81	2.6689253501665995
2205	82-83	2.7923632649440435
2205	84-85	2.1239355537035287
2205	86-87	1.9178801392040654
2205	88-89	1.894665709718673
2205	90-91	1.81820313525321
2205	92-93	1.5726559420036175
2205	94-95	1.9817003324771605
2205	96-97	1.7717714496532402
2205	98-99	1.6071920768397767
2205	100-101	1.1577262590470312
2206	1	8.110635397936846
2206	2	8.3257256780362
2206	3	7.306854998012618
2206	4	8.778608961512912
2206	5	8.79574012982006
2206	6	7.641573759100073
2206	7	8.011135529627346
2206	8	8.671120247975306
2206	9	7.371246490688755
2206	10-11	7.826050441353136
2206	12-13	7.510994398935335
2206	14-15	8.28500727415226
2206	16-17	7.075666215547603
2206	18-19	6.499822163929046
2206	20-21	6.364133109041397
2206	22-23	5.874896040596745
2206	24-25	6.154812506409616
2206	26-27	6.780161259114539
2206	28-29	5.817528386380985
2206	30-31	5.732415218815095
2206	32-33	5.920430856095663
2206	34-35	5.575522430266297
2206	36-37	5.6794521976407815
2206	38-39	5.141815319590581
2206	40-41	4.47600735434289
2206	42-43	4.301449833508052
2206	44-45	4.238434663463742
2206	46-47	4.599669296616238
2206	48-49	4.274750530036723
2206	50-51	4.427126988270583
2206	52-53	5.015754961430563
2206	54-55	4.567841524327932
2206	56-57	4.80772232412567
2206	58-59	4.395689572034232
2206	60-61	4.3176946788344175
2206	62-63	3.8447370111092987
2206	64-65	3.714561856245087
2206	66-67	3.3068036957372016
2206	68-69	3.6543087652631385
2206	70-71	3.5708202865585044
2206	72-73	2.7842358161348066
2206	74-75	2.3503482799438515
2206	76-77	2.6770213582138247
2206	78-79	3.3483291950745304
2206	80-81	2.9259565725928915
2206	82-83	2.977024537239938
2206	84-85	2.4230910383799014
2206	86-87	2.3772988282151033
2206	88-89	2.451481309820636
2206	90-91	2.55069321688773
2206	92-93	2.0897150731090512
2206	94-95	2.422524390150741
2206	96-97	2.238354956734984
2206	98-99	2.005366836151925
2206	100-101	1.7286725397416731
2309	1	8.038962828082457
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2309	14-15	7.529749501621762
2309	16-17	6.801297856633784
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2309	24-25	6.000495308456056
2309	26-27	6.699590617634808
2309	28-29	5.813207252968937
2309	30-31	5.796767835894986
2309	32-33	5.871875169358155
2309	34-35	5.539965562891247
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2309	66-67	3.7801567063629378
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2207	1	7.203851248902911
2207	2	7.481189047483216
2207	3	6.725667090909852
2207	4	7.559020430506276
2207	5	7.46727839702227
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2207	86-87	2.183582536049421
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2207	90-91	2.142222001044086
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2207	96-97	1.8664003874812884
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2104	9	-24.727974288532025
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2104	16-17	-10.916708030827635
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2104	20-21	-1.176280207291999
2104	22-23	-2.579874556344542
2104	24-25	-1.6968233959218928
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2104	30-31	-3.8676233513961407
2104	32-33	-1.6980240289305115
2104	34-35	-4.018713821888063
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2104	38-39	-2.6692490772853574
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2104	78-79	-0.731891206132552
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2104	96-97	-3.6763437535335335
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2104	100-101	-1.8479659574296292
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2110	2	-20.388560036249515
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2110	4	-23.317494934590986
2110	5	-11.995223643248975
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2110	34-35	-20.15503524735021
2110	36-37	-26.165093256904672
2110	38-39	-15.740910746912574
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2110	44-45	-9.033253246149862
2110	46-47	-4.36284769104007
2110	48-49	-2.476680051765374
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2110	76-77	-3.2264284427591967
2110	78-79	-2.6386307180773336
2110	80-81	-2.604009251057146
2110	82-83	-3.071823920803567
2110	84-85	-3.067088689013449
2110	86-87	-2.8451007536272
2110	88-89	-2.8943620418741105
2110	90-91	-2.4977346710138413
2110	92-93	-2.1393979500906575
2110	94-95	-2.2565238990213885
2110	96-97	-2.26891616223892
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2109	1	7.802889393518541
2109	2	7.834977332705506
2109	3	6.943094517708342
2109	4	8.665934216575739
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2109	14-15	7.249706534986114
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2109	20-21	2.0493046807905806
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2109	32-33	5.241203401102226
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2109	36-37	4.9772807708261375
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2109	42-43	3.553254180665796
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2109	58-59	3.431461919402789
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2109	62-63	2.835746941321119
2109	64-65	2.8565525980024837
2109	66-67	2.868728997354758
2109	68-69	2.8756758493119925
2109	70-71	2.5840951483216976
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2109	74-75	1.5324434184880928
2109	76-77	1.9122791001337198
2109	78-79	2.456957543391752
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2109	84-85	1.4048813751989861
2109	86-87	1.4498952206626967
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2108	1	6.710401406221479
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2108	7	6.319786022231916
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2108	84-85	1.8174550166027466
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2211	1	6.430123290292865
2211	2	5.997897681077511
2211	3	5.489856833551414
2211	4	7.438270884451004
2211	5	7.069412666626839
2211	6	6.815761936387009
2211	7	6.818006127982272
2211	8	6.393793990554432
2211	9	5.42134564050405
2211	10-11	5.688404539059096
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2211	14-15	6.272591121410851
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2211	26-27	4.91409676181409
2211	28-29	4.634916007630224
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2211	32-33	5.146453189764358
2211	34-35	4.540054675076483
2211	36-37	4.85944843440225
2211	38-39	4.340760383988037
2211	40-41	3.7990129531202186
2211	42-43	3.610490745286988
2211	44-45	3.7934973713058753
2211	46-47	4.2370096853818175
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2211	50-51	3.328128086521833
2211	52-53	4.017255956000749
2211	54-55	3.412032159240031
2211	56-57	3.7307300733250237
2211	58-59	3.5298690708322944
2211	60-61	0.5036472773890246
2211	62-63	1.240564654784368
2211	64-65	2.6110554395393812
2211	66-67	2.8773201042252197
2211	68-69	3.0170603838397128
2211	70-71	2.9751689006883133
2211	72-73	2.506893737751927
2211	74-75	2.054523401097093
2211	76-77	2.1927179029464376
2211	78-79	2.616075860000379
2211	80-81	2.828249767275203
2211	82-83	2.8430680679256923
2211	84-85	2.222162363270268
2211	86-87	2.185156316675787
2211	88-89	2.16301264070146
2211	90-91	2.0893144123687684
2211	92-93	1.9642146007842136
2211	94-95	2.2520456908488597
2211	96-97	1.9503571532374764
2211	98-99	1.9455438619646728
2211	100-101	1.7896790140479766
2210	1	7.324564247055569
2210	2	7.401723918591401
2210	3	6.634626501227157
2210	4	8.329101028460673
2210	5	8.318613978516694
2210	6	7.27143082642549
2210	7	7.757669290085161
2210	8	7.388780630698985
2210	9	6.909726703599347
2210	10-11	7.086067813648992
2210	12-13	7.062188155572134
2210	14-15	4.8378927636200295
2210	16-17	1.436366972197721
2210	18-19	5.100416954017504
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2210	22-23	5.121840960199897
2210	24-25	5.663920654505109
2210	26-27	4.526338109974933
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2210	30-31	5.367303409652653
2210	32-33	5.402262455901948
2210	34-35	5.279208378256037
2210	36-37	5.002371676383046
2210	38-39	4.32585777730392
2210	40-41	4.1926657421405125
2210	42-43	4.43483272157448
2210	44-45	4.4506861307785
2210	46-47	4.6398412423578
2210	48-49	4.481317213438846
2210	50-51	4.522745481990345
2210	52-53	4.901310275087145
2210	54-55	4.584795463307181
2210	56-57	4.743533512595086
2210	58-59	4.370632279002255
2210	60-61	4.268866708993787
2210	62-63	3.744283554200141
2210	64-65	3.5123588625231044
2210	66-67	3.38864538124977
2210	68-69	3.671743930166656
2210	70-71	3.847171126959978
2210	72-73	3.1495743537518273
2210	74-75	2.761839942574756
2210	76-77	2.95168535313098
2210	78-79	3.658199058362115
2210	80-81	3.263680757405684
2210	82-83	3.0417387385364165
2210	84-85	2.517141538000022
2210	86-87	2.682562944871627
2210	88-89	2.8457028221434086
2210	90-91	2.822095352213914
2210	92-93	2.395903475879731
2210	94-95	2.7502529291451054
2210	96-97	2.8518225291898993
2210	98-99	2.7944429054811586
2210	100-101	2.342128560286305
2209	1	5.22879791109936
2209	2	2.648939963750486
2209	3	4.512410456068075
2209	4	2.040275335679283
2209	5	4.981216612796544
2209	6	5.228157648183959
2209	7	6.081313662305476
2209	8	7.004381041236105
2209	9	6.089255441197707
2209	10-11	6.0105204308231315
2209	12-13	5.552978867169802
2209	14-15	4.653747621642609
2209	16-17	4.462433941065317
2209	18-19	3.8600731291800088
2209	20-21	4.091260609918898
2209	22-23	3.885166310867021
2209	24-25	4.140665437262552
2209	26-27	1.9731671383704175
2209	28-29	0.48492659627919465
2209	30-31	3.077154162304044
2209	32-33	4.293252021416826
2209	34-35	4.179563321807187
2209	36-37	3.8389607971493795
2209	38-39	-1.0556359791107148
2209	40-41	-0.33987146278592917
2209	42-43	-4.635567979759763
2209	44-45	0.45699600077508507
2209	46-47	2.4231503425972782
2209	48-49	1.782377767663963
2209	50-51	3.084969652363249
2209	52-53	3.744277688703292
2209	54-55	3.1956640059004116
2209	56-57	3.402635890289236
2209	58-59	2.787075145919804
2209	60-61	2.9020304106701467
2209	62-63	2.7104741118463984
2209	64-65	2.219536847470078
2209	66-67	2.321509732943234
2209	68-69	-0.7323400588856899
2209	70-71	1.8352933472815671
2209	72-73	2.1096688628178555
2209	74-75	1.8044716565672303
2209	76-77	2.1902694277118933
2209	78-79	2.4703847408800748
2209	80-81	2.006534845671162
2209	82-83	2.0677287316941317
2209	84-85	1.5504506382395
2209	86-87	1.7475625172287934
2209	88-89	1.7327580735973953
2209	90-91	1.7447262109207209
2209	92-93	1.6099002257942026
2209	94-95	1.8524638425901934
2209	96-97	1.5787362308662587
2209	98-99	1.5168107638458572
2209	100-101	1.1417394053085417
2208	1	4.429867117560725
2208	2	6.105993340656802
2208	3	4.56248921129389
2208	4	2.8128609114511356
2208	5	3.7322693812512746
2208	6	4.783256129017733
2208	7	3.182997944627015
2208	8	3.96493571355548
2208	9	3.959389545890634
2208	10-11	4.189232966888607
2208	12-13	4.939068510734938
2208	14-15	5.467848855229132
2208	16-17	3.788030002813347
2208	18-19	1.841816146707341
2208	20-21	4.075087942153097
2208	22-23	3.198193063109457
2208	24-25	3.644579645588525
2208	26-27	4.90502313652544
2208	28-29	4.004411031695
2208	30-31	4.1167612939454905
2208	32-33	3.277033106423403
2208	34-35	3.6102152973121022
2208	36-37	3.72815293045916
2208	38-39	3.6646027645348873
2208	40-41	3.0848217353141294
2208	42-43	3.0283158795897833
2208	44-45	-1.3562035958803946
2208	46-47	2.633030075271133
2208	48-49	2.7651595690732123
2208	50-51	2.9638579769623554
2208	52-53	1.5853225325667637
2208	54-55	0.5843550253512362
2208	56-57	-1.3981903035516652
2208	58-59	1.8427575440041615
2208	60-61	2.8542578832407557
2208	62-63	2.405879811663862
2208	64-65	2.6753040116931253
2208	66-67	2.883085279077605
2208	68-69	1.7850923244780716
2208	70-71	-0.29050831222107476
2208	72-73	-0.11341586406589244
2208	74-75	-2.305831538784986
2208	76-77	0.4035853171307231
2208	78-79	1.7163820862646766
2208	80-81	1.8944848054348462
2208	82-83	2.1472488155034313
2208	84-85	1.3400473427871873
2208	86-87	1.7218239109754805
2208	88-89	2.11997691008094
2208	90-91	1.9173782747492574
2208	92-93	1.7376497890829832
2208	94-95	1.628022980159134
2208	96-97	1.8779534628040757
2208	98-99	2.0875222841897276
2208	100-101	1.9676528187219553
1307	1	7.409989924007792
1307	2	7.233828023451977
1307	3	7.024123848565051
1307	4	8.756758796752298
1307	5	5.0042853905295
1307	6	5.957156236324341
1307	7	3.9611694509299262
1307	8	5.731458491821016
1307	9	5.8824734726620065
1307	10-11	7.008402641391907
1307	12-13	5.997989456116958
1307	14-15	5.806190643022195
1307	16-17	4.539786200040712
1307	18-19	5.454390394206229
1307	20-21	5.345075152072994
1307	22-23	4.901090070447495
1307	24-25	5.543363097609461
1307	26-27	6.401961511101359
1307	28-29	5.733297915359472
1307	30-31	5.23827003564006
1307	32-33	5.875941613994478
1307	34-35	5.656070986187984
1307	36-37	5.277817190856521
1307	38-39	4.732703573128088
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1212	60-61	-14.377045580905845
1212	62-63	-12.116074677202393
1212	64-65	-16.520632948949718
1212	66-67	-12.81293656400306
1212	68-69	-8.026048377594005
1212	70-71	-15.506679713441496
1212	72-73	-12.034660287761296
1212	74-75	-8.336924447328876
1212	76-77	-19.530933187240723
1212	78-79	-10.174203272457937
1212	80-81	-7.912809661173345
1212	82-83	-5.204241696526296
1212	84-85	-4.289831039542175
1212	86-87	-3.56977909386282
1212	88-89	-3.060401807062483
1212	90-91	-3.620573016878506
1212	92-93	-3.64570700481303
1212	94-95	-3.551111973485625
1212	96-97	-13.441093095213068
1212	98-99	-10.874600696315603
1212	100-101	-6.977723564154431
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	324.0
3	602.0
4	278.0
5	1705.0
6	2341.0
7	1985.0
8	4080.0
9	7939.0
10	10335.0
11	12116.0
12	16096.0
13	20888.0
14	24244.0
15	25893.0
16	27111.0
17	29912.0
18	34563.0
19	38298.0
20	41023.0
21	46600.0
22	52318.0
23	56889.0
24	59570.0
25	62194.0
26	65730.0
27	69696.0
28	71018.0
29	72285.0
30	73746.0
31	78929.0
32	86434.0
33	100828.0
34	116754.0
35	130701.0
36	139365.0
37	131799.0
38	48207.0
39	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.811291170523624	20.752898229841026	15.11080025292685	21.3250103467085
2	23.897586877393046	30.025827982825604	26.314922650106798	19.761662489674553
3	18.611410463775393	28.03299820514224	30.168017056496183	23.187574274586186
4	21.393289080710254	28.207383065464825	28.351891921774943	22.04743593204998
5	20.779677600138452	29.027608479287547	28.070505367187337	22.12220855338666
6	20.673134535832922	29.17497519425178	28.00598932993323	22.14590093998207
7	21.76506295034738	28.312150690834066	29.28249499133509	20.640291367483464
8	20.680653234122712	28.130290666577416	29.72087698215484	21.468179117145027
9	21.148020671592874	27.954029275360753	29.395187383539373	21.502762669507003
10-11	21.9477083236139	28.49077203928813	27.94671588084143	21.61480375625654
12-13	21.519003663425448	28.758594446478515	28.04114239381179	21.681259496284248
14-15	21.543373688478212	28.92563578422348	28.15851941210005	21.37247111519826
16-17	21.87449828831635	28.745679269906972	27.89763173145929	21.482190710317393
18-19	21.903953570388538	28.82753816819027	27.774033192021154	21.494475069400035
20-21	21.87415582093204	28.87905312046631	27.858004361415027	21.38878669718662
22-23	21.6968736099247	28.74158121348833	28.03033120506918	21.531213971517786
24-25	21.699514346372176	28.75332246395661	28.145013592599415	21.402149597071794
26-27	21.603854251905517	28.83983019604924	28.081982339226485	21.47433321281876
28-29	21.750151390687265	28.75539825420127	28.16406536383122	21.330384991280244
30-31	21.78005674000855	28.60446736172887	28.00491963379541	21.610556264467164
32-33	21.591863790392033	28.93942248286966	28.089019264612276	21.379694462126032
34-35	21.804859441772535	28.656461649862504	28.119546411969576	21.419132496395385
36-37	21.78945557089027	28.695724190567162	28.019006849114625	21.495813389427944
38-39	21.53174843741103	28.808636269779324	28.33708439044507	21.322530902364576
40-41	21.881894709597592	28.653728010458508	27.963772833872163	21.50060444607173
42-43	21.62791340997764	28.90610896731528	28.00774903529188	21.458228587415203
44-45	21.5686730842608	28.630009148289638	28.35081966836301	21.450498099086552
46-47	21.80207848076514	28.677973510009952	27.99317908636565	21.52676892285926
48-49	21.612233730084064	28.66188191124025	28.231828438223083	21.494055920452602
50-51	21.817208658573634	28.67358450214239	28.07617612522431	21.43303071405966
52-53	21.601470696853717	28.758264963881043	28.191051012034613	21.44921332723063
54-55	21.539142151384574	28.615726165786203	28.269200003927295	21.575931678901927
56-57	21.53791365298178	28.77211487749916	28.230330344728387	21.45964112479067
58-59	21.60326760251737	28.692330918222353	28.22438635936713	21.480015119893146
60-61	21.69811347885267	28.415726579101225	28.174299956258498	21.71185998578761
62-63	21.634328534949805	28.732063129907324	28.212486335749503	21.421121999393367
64-65	21.715906767532204	28.58440405330589	28.20463635340306	21.495052825758844
66-67	21.851321341518023	28.542825018867347	28.067590439176993	21.538263200437644
68-69	21.651288332192607	28.728209737724917	28.226862882664367	21.3936390474181
70-71	21.886235735780897	28.511622643995743	28.074446798240526	21.52769482198283
72-73	21.724448804503577	28.694781100052	28.10177331780062	21.478996777643797
74-75	21.658912514596704	28.584415588102775	28.25646705367868	21.50020484362184
76-77	21.941457232598317	28.634904335775275	27.992470174734795	21.43116825689161
78-79	21.802363006232802	28.59367427499973	28.15277775393677	21.4511849648307
80-81	21.889205414146357	28.556990901992684	28.060262742828044	21.49354094103291
82-83	21.84585922950994	28.620706660254804	28.10327247476468	21.430161635470583
84-85	21.885415626050076	28.343699766599155	28.12775290388789	21.64313170346287
86-87	21.872547850625423	28.551876266884697	28.144287166209597	21.431288716280285
88-89	21.927333151290583	28.48598147819092	28.059354262951103	21.527331107567395
90-91	22.04350309300826	28.40531806662004	27.880874898943564	21.67030394142814
92-93	22.012316851677024	28.581181302954377	27.949883703851224	21.45661814151737
94-95	22.163236652461126	28.429145234419916	27.88374476979103	21.523873343327928
96-97	22.2200618874241	28.45338373903662	27.773504553308868	21.553049820230413
98-99	22.155032295911663	28.509801729014043	27.852786609719683	21.482379365354614
100-101	22.550601486959682	28.332085387894985	27.492039017767446	21.625274107377887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	134.0
1	140.0
2	150.0
3	150.5
4	162.0
5	182.0
6	221.5
7	288.5
8	343.0
9	444.5
10	627.5
11	894.0
12	1162.5
13	1522.0
14	2008.5
15	2517.5
16	3160.0
17	3993.5
18	5060.0
19	6436.0
20	8036.0
21	10162.5
22	12872.0
23	15749.5
24	18931.5
25	22641.0
26	26513.0
27	30588.5
28	34679.0
29	38410.0
30	42412.5
31	46165.0
32	49643.5
33	53157.0
34	55940.5
35	58390.0
36	60869.5
37	62351.5
38	62819.0
39	62240.5
40	60262.0
41	57797.0
42	55004.5
43	51845.0
44	48599.5
45	45509.0
46	42893.5
47	40549.0
48	38227.5
49	36218.0
50	33993.5
51	31591.0
52	29328.5
53	27268.0
54	25561.0
55	24071.0
56	22997.5
57	21886.5
58	20552.5
59	19525.5
60	18662.5
61	17671.5
62	16755.0
63	16052.0
64	15531.0
65	14920.5
66	14355.0
67	13932.5
68	13282.0
69	12310.5
70	11258.5
71	10211.0
72	9141.5
73	8058.0
74	6862.0
75	5641.5
76	4597.5
77	3674.0
78	2931.0
79	2338.0
80	1888.5
81	1512.5
82	1166.5
83	928.5
84	739.5
85	611.5
86	495.5
87	393.0
88	326.0
89	259.5
90	191.0
91	138.0
92	99.0
93	70.5
94	48.0
95	35.5
96	23.5
97	14.5
98	8.5
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	13.790505881425338
2	11.140872289941194
3	9.834916215852877
4	11.75939051223451
5	9.209931460421132
6	5.32950106158919
7	9.82334452497358
8	10.173785241896555
9	5.417933738455962
10-11	6.378610977337071
12-13	5.416827620945441
14-15	7.414192225071657
16-17	2.661630712175177
18-19	3.0931867132030155
20-21	3.093300161152813
22-23	3.1485493127039583
24-25	3.5063074223888395
26-27	4.3732199307627155
28-29	1.738589830639228
30-31	2.247630781378427
32-33	3.044659352677343
34-35	2.00099493851972
36-37	3.084706478955689
38-39	1.378506037983508
40-41	1.1582184814651575
42-43	0.5486626472058054
44-45	1.28803129802039
46-47	1.3700541657236305
48-49	1.2663911015966096
50-51	1.5984816126399168
52-53	2.465564292938489
54-55	1.7840824585078303
56-57	1.8033686099733228
58-59	1.5576970746878904
60-61	1.3783642280462618
62-63	0.6030609391334733
64-65	1.2324418026198536
66-67	0.5612270076458246
68-69	0.796064263725642
70-71	0.9935487823347928
72-73	0.08389475887489131
74-75	0.10343616822742685
76-77	0.45577713830950073
78-79	0.86413303360385
80-81	0.29045511346780317
82-83	0.5039074310108836
84-85	0.0650623992085871
86-87	0.09716816900114185
88-89	0.0812287320546615
90-91	0.08684440556961365
92-93	0.16883891128540499
94-95	0.45231697584069186
96-97	0.3691596286394811
98-99	0.06622524069400648
100-101	0.22871106679077874
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1762923.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.79520106956069
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.08652405238932	97.89273067039665
2	0.6974147381701405	1.3780245857278812
3	0.15250899026323525	0.45201469073916
4	0.047767277278277336	0.1887671105301147
5	0.010213473778360719	0.05045210977759165
6	0.0021326312278994123	0.012641623858052793
7	0.0018321108628454935	0.012670263275358279
8	0.001606726029923144	0.01269894569519632
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	5.672397489850663E-5	0.0	0.0
2	5.672397489850663E-5	0.0	5.672397489850663E-5
3	5.672397489850663E-5	0.0	5.672397489850663E-5
4	5.672397489850663E-5	0.0	5.672397489850663E-5
5	5.672397489850663E-5	0.0	5.672397489850663E-5
6	1.7017192469551988E-4	0.0	5.672397489850663E-5
7	1.7017192469551988E-4	0.0	5.672397489850663E-5
8	1.7017192469551988E-4	0.0	5.672397489850663E-5
9	2.268958995940265E-4	0.0	5.672397489850663E-5
10-11	2.268958995940265E-4	0.0	5.672397489850663E-5
12-13	2.8361987449253317E-4	0.0	5.672397489850663E-5
14-15	3.4034384939103977E-4	0.0	5.672397489850663E-5
16-17	3.970678242895464E-4	0.0	5.672397489850663E-5
18-19	4.53791799188053E-4	0.0	1.7017192469551988E-4
20-21	5.105157740865596E-4	0.0	1.7017192469551988E-4
22-23	5.105157740865596E-4	0.0	1.7017192469551988E-4
24-25	5.105157740865596E-4	0.0	1.7017192469551988E-4
26-27	5.105157740865596E-4	0.0	2.268958995940265E-4
28-29	5.105157740865596E-4	0.0	2.268958995940265E-4
30-31	6.239637238835729E-4	0.0	2.268958995940265E-4
32-33	6.806876987820795E-4	0.0	2.268958995940265E-4
34-35	8.508596234775994E-4	0.0	2.268958995940265E-4
36-37	0.0010493935356223727	0.0	2.268958995940265E-4
38-39	0.0013897373850134125	0.0	2.268958995940265E-4
40-41	0.002070425083795492	0.0	2.552578870432798E-4
42-43	0.002694388807679065	0.0	2.8361987449253317E-4
44-45	0.0036586963809536774	0.0	2.8361987449253317E-4
46-47	0.005161881715764103	0.0	2.8361987449253317E-4
48-49	0.007260668787008848	0.0	3.4034384939103977E-4
50-51	0.009955057594687913	0.0	3.4034384939103977E-4
52-53	0.01446461359911919	0.0	3.4034384939103977E-4
54-55	0.020165373076419107	0.0	3.4034384939103977E-4
56-57	0.026915526089341394	0.0	3.970678242895464E-4
58-59	0.03567938021116067	5.672397489850663E-5	3.970678242895464E-4
60-61	0.048158654688832125	5.672397489850663E-5	3.970678242895464E-4
62-63	0.06353085188632743	5.672397489850663E-5	3.970678242895464E-4
64-65	0.08066149230567643	5.672397489850663E-5	4.254298117387997E-4
66-67	0.09986255780882092	5.672397489850663E-5	4.53791799188053E-4
68-69	0.12422550502772951	8.508596234775994E-5	4.53791799188053E-4
70-71	0.15289947433892462	1.1344794979701326E-4	4.53791799188053E-4
72-73	0.18803997678854947	1.1344794979701326E-4	4.53791799188053E-4
74-75	0.22871106679077874	1.1344794979701326E-4	4.53791799188053E-4
76-77	0.27386335080999	1.1344794979701326E-4	4.53791799188053E-4
78-79	0.327382421126731	1.1344794979701326E-4	4.53791799188053E-4
80-81	0.3936076618207375	1.1344794979701326E-4	4.53791799188053E-4
82-83	0.4689371004859543	1.1344794979701326E-4	4.53791799188053E-4
84-85	0.5544201306580038	1.4180993724626656E-4	4.53791799188053E-4
86-87	0.6578846608728799	1.7017192469551988E-4	4.53791799188053E-4
88-89	0.7713893346447916	1.7017192469551988E-4	5.105157740865596E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAGAT	450	0.0038894513	9.673227	3
GTTGATC	635	0.005260927	8.004906	1
GTTGCAT	780	0.004051989	7.240905	1
AGAGCGT	1600	0.0	7.087643	94-95
GTCCCAG	1335	1.1142221E-5	6.7690253	1
GTCATAT	835	0.007848959	6.763959	1
GTCATTT	1840	1.2488636E-7	6.44598	1
AAGAGCG	1910	0.0	6.2935305	94-95
GATTCTT	1300	3.9329793E-4	6.0823603	1
CGTGTAG	710	2.8155755E-6	6.0694184	94-95
GTTATTA	1120	0.0030826624	6.051328	1
GTCTCTT	1035	0.009105714	6.0026054	1
GAGCGTC	1605	0.0	5.9350734	94-95
TCGTGTA	705	1.6199825E-5	5.7907553	94-95
TCGGAAG	2280	0.0	5.6700335	92-93
GTCGTGT	850	1.7159637E-6	5.6034074	94-95
AGCGTCG	1455	9.094947E-12	5.4557805	94-95
GCGTCGT	1455	9.094947E-12	5.4557805	94-95
GAGCCAC	1660	1.1062722E-4	5.4317403	6
CGGAAGA	2365	0.0	5.3608265	90-91
>>END_MODULE