FastQCFastQC Report
Thu 11 Sep 2014
LONLib30_CACCGG_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib30_CACCGG_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762923
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGAT4500.00388945139.6732273
GTTGATC6350.0052609278.0049061
GTTGCAT7800.0040519897.2409051
AGAGCGT16000.07.08764394-95
GTCCCAG13351.1142221E-56.76902531
GTCATAT8350.0078489596.7639591
GTCATTT18401.2488636E-76.445981
AAGAGCG19100.06.293530594-95
GATTCTT13003.9329793E-46.08236031
CGTGTAG7102.8155755E-66.069418494-95
GTTATTA11200.00308266246.0513281
GTCTCTT10350.0091057146.00260541
GAGCGTC16050.05.935073494-95
TCGTGTA7051.6199825E-55.790755394-95
TCGGAAG22800.05.670033592-93
GTCGTGT8501.7159637E-65.603407494-95
AGCGTCG14559.094947E-125.455780594-95
GCGTCGT14559.094947E-125.455780594-95
GAGCCAC16601.1062722E-45.43174036
CGGAAGA23650.05.360826590-91
Produced by FastQC (version 0.11.2)