##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib30_CACCGG_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1762923 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2292034308929 33.0 33.0 33.0 27.0 33.0 2 31.59671409358208 33.0 33.0 33.0 27.0 33.0 3 31.791250667215756 33.0 33.0 33.0 27.0 33.0 4 35.62018136923734 37.0 37.0 37.0 33.0 37.0 5 35.14582032227159 37.0 37.0 37.0 33.0 37.0 6 34.95605366768713 37.0 33.0 37.0 33.0 37.0 7 34.761804117366445 37.0 33.0 37.0 27.0 37.0 8 34.80070088143385 37.0 33.0 37.0 27.0 37.0 9 34.704978606552864 37.0 33.0 37.0 27.0 37.0 10-11 34.55681388239872 37.0 33.0 37.0 27.0 37.0 12-13 34.56751259130433 37.0 33.0 37.0 27.0 37.0 14-15 35.51173902660524 37.0 33.0 40.0 27.0 40.0 16-17 35.41427277311601 37.0 33.0 40.0 27.0 40.0 18-19 35.63140619301013 37.0 33.0 40.0 27.0 40.0 20-21 35.32900359232933 37.0 33.0 40.0 27.0 40.0 22-23 35.35305285596705 37.0 33.0 40.0 27.0 40.0 24-25 35.208425155267705 37.0 33.0 40.0 27.0 40.0 26-27 34.84357030908326 37.0 33.0 40.0 27.0 40.0 28-29 34.50246777652796 37.0 33.0 40.0 27.0 40.0 30-31 34.41626293377533 37.0 33.0 40.0 27.0 40.0 32-33 34.41961418621233 37.0 33.0 40.0 27.0 40.0 34-35 34.28905686748655 37.0 33.0 40.0 24.5 40.0 36-37 34.00276812997505 37.0 33.0 37.0 22.0 40.0 38-39 33.94062247755574 37.0 33.0 37.0 22.0 40.0 40-41 33.68845689800405 37.0 33.0 37.0 22.0 40.0 42-43 33.34890463168272 37.0 33.0 37.0 22.0 40.0 44-45 33.246308261903664 37.0 33.0 37.0 22.0 40.0 46-47 33.13896608076473 37.0 33.0 37.0 22.0 40.0 48-49 33.18486371781411 37.0 33.0 37.0 22.0 40.0 50-51 33.46624866769564 37.0 33.0 37.0 22.0 40.0 52-53 33.329694206723715 37.0 33.0 37.0 22.0 40.0 54-55 32.994304629300316 37.0 33.0 37.0 22.0 40.0 56-57 33.05929215286204 37.0 33.0 37.0 22.0 40.0 58-59 32.77587137952139 37.0 33.0 37.0 22.0 40.0 60-61 32.70810523204928 37.0 33.0 37.0 22.0 40.0 62-63 32.691066200849384 37.0 33.0 37.0 22.0 40.0 64-65 32.3642135816482 37.0 30.0 37.0 22.0 40.0 66-67 32.01999718649084 33.0 27.0 37.0 22.0 40.0 68-69 31.84749362280712 33.0 27.0 37.0 22.0 40.0 70-71 31.69532163344627 33.0 27.0 37.0 22.0 40.0 72-73 31.467062089495684 33.0 27.0 37.0 18.5 38.5 74-75 31.1090960864428 33.0 27.0 37.0 15.0 37.0 76-77 30.060285673282387 33.0 27.0 37.0 15.0 37.0 78-79 30.947166722539784 33.0 27.0 37.0 18.5 37.0 80-81 30.649130166206916 33.0 27.0 37.0 15.0 37.0 82-83 30.615050969327644 33.0 27.0 37.0 15.0 37.0 84-85 30.47832009679379 33.0 27.0 37.0 15.0 37.0 86-87 30.263343322425314 33.0 27.0 37.0 15.0 37.0 88-89 30.120956218734456 33.0 27.0 37.0 15.0 37.0 90-91 30.02044615675217 33.0 27.0 37.0 15.0 37.0 92-93 29.80070995726983 33.0 27.0 37.0 15.0 37.0 94-95 29.714339764130365 33.0 27.0 35.0 15.0 37.0 96-97 29.579361946040752 33.0 27.0 33.0 15.0 37.0 98-99 29.315739258039066 33.0 27.0 33.0 15.0 37.0 100-101 28.545539425147894 33.0 27.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.1055767688431857 2306 2 0.5328836721455978 2306 3 0.40686772512268377 2306 4 0.3539279142719849 2306 5 0.6650924475016495 2306 6 0.6620040193222252 2306 7 0.8276413463027481 2306 8 0.7103656645448808 2306 9 1.0741955377501426 2306 10-11 1.1265585222583994 2306 12-13 1.3245230949339444 2306 14-15 1.552228742398178 2306 16-17 1.4483965741895162 2306 18-19 1.2534164938594259 2306 20-21 1.520100109196548 2306 22-23 1.227773438527123 2306 24-25 1.335947183298643 2306 26-27 1.4376254991138921 2306 28-29 1.7628775658965097 2306 30-31 1.671616116656807 2306 32-33 1.2422419011636876 2306 34-35 1.1154988554433487 2306 36-37 1.3551326135259032 2306 38-39 1.0669237244224021 2306 40-41 1.307416586570703 2306 42-43 1.27174304033079 2306 44-45 1.1585087693780949 2306 46-47 1.1736073401752947 2306 48-49 1.163948536643602 2306 50-51 1.1994238340784023 2306 52-53 1.258823939799946 2306 54-55 1.66769304450861 2306 56-57 1.297766356436064 2306 58-59 1.148154275229352 2306 60-61 1.0544933643148937 2306 62-63 0.814683533708866 2306 64-65 0.9244998505160922 2306 66-67 1.0891959435754437 2306 68-69 0.8138576804650093 2306 70-71 0.8029453416321566 2306 72-73 0.5533749093138915 2306 74-75 0.8950058206784988 2306 76-77 0.9450449703606054 2306 78-79 0.8373760388390288 2306 80-81 1.4904878700126645 2306 82-83 1.3281143025402464 2306 84-85 0.9978249903992129 2306 86-87 1.0327136447438434 2306 88-89 0.8986987073015129 2306 90-91 0.5679078071046284 2306 92-93 0.4236036813906381 2306 94-95 0.3587102053940754 2306 96-97 0.4302339519266418 2306 98-99 0.5582755423753412 2306 100-101 0.4808438876376826 2307 1 0.5644206957730518 2307 2 0.398401049936318 2307 3 0.4539734501049999 2307 4 0.37475432188428925 2307 5 0.5440098592588143 2307 6 0.5732004633495578 2307 7 0.8884863604315569 2307 8 0.8201752137529112 2307 9 1.059204401081601 2307 10-11 1.192385553952903 2307 12-13 1.2812914881371071 2307 14-15 1.269055431017982 2307 16-17 1.3435814559856212 2307 18-19 1.2346760554467906 2307 20-21 1.3858695411726103 2307 22-23 1.2329245721400568 2307 24-25 1.2749482288921143 2307 26-27 1.4047734366074565 2307 28-29 1.5194618719000417 2307 30-31 1.6661853737534358 2307 32-33 1.4485824399751195 2307 34-35 1.483704799843423 2307 36-37 1.5378761948574606 2307 38-39 1.1910115242632102 2307 40-41 1.3120812088850187 2307 42-43 1.2875828264922617 2307 44-45 1.1005362044411484 2307 46-47 1.0391705180973787 2307 48-49 1.111134642050068 2307 50-51 1.00531806885472 2307 52-53 1.2250755861151745 2307 54-55 1.613493755621299 2307 56-57 1.369454577242486 2307 58-59 1.2847265156435483 2307 60-61 1.020756878422901 2307 62-63 1.0261539941587685 2307 64-65 1.0399376663511433 2307 66-67 1.157510300944697 2307 68-69 1.1273149731213614 2307 70-71 1.0222873543688955 2307 72-73 0.840693115531117 2307 74-75 1.1676572765633928 2307 76-77 1.1530865594716992 2307 78-79 1.0394625536843414 2307 80-81 1.6556292638508268 2307 82-83 1.2781794048863198 2307 84-85 1.2808281626465856 2307 86-87 1.3152695239733632 2307 88-89 1.0208410757992077 2307 90-91 0.6580667809634271 2307 92-93 0.8247625986386993 2307 94-95 0.7564085697530487 2307 96-97 0.6148268627620084 2307 98-99 0.6821705246861924 2307 100-101 0.8373331148874996 1101 1 1.0740463863798162 1101 2 0.4678773370264757 1101 3 0.31979920405762385 1101 4 0.2528556176651833 1101 5 0.5033440676357728 1101 6 0.4840596424237944 1101 7 0.5814947196966571 1101 8 0.6948804834679052 1101 9 0.8962256991940762 1101 10-11 0.9578545485446313 1101 12-13 1.2324996944451456 1101 14-15 1.2368833213130799 1101 16-17 1.296650745254965 1101 18-19 0.9986558957337692 1101 20-21 1.2210254021741136 1101 22-23 0.991875299318167 1101 24-25 1.1351504580762892 1101 26-27 1.1278069937581279 1101 28-29 1.291793533099444 1101 30-31 1.3407733623399523 1101 32-33 0.8160860865754742 1101 34-35 0.9545118063725369 1101 36-37 1.0756950397577327 1101 38-39 0.8210621798787585 1101 40-41 1.0951935340079686 1101 42-43 1.1256880620763354 1101 44-45 0.9229202207790408 1101 46-47 0.8139657150489086 1101 48-49 0.8141263291355898 1101 50-51 0.8395294305167127 1101 52-53 1.2015391246764366 1101 54-55 1.4372222210152898 1101 56-57 1.1673365643359475 1101 58-59 1.0710161410392018 1101 60-61 0.8527773302853205 1101 62-63 0.613510772481284 1101 64-65 0.4691697418268177 1101 66-67 0.7901058165918933 1101 68-69 0.6135235200362708 1101 70-71 0.8604405558276689 1101 72-73 0.575272683753969 1101 74-75 0.9032731324101348 1101 76-77 0.9333109245910265 1101 78-79 0.7053674197275193 1101 80-81 1.3671302235194638 1101 82-83 1.2009814547076232 1101 84-85 1.017288793759512 1101 86-87 0.7549452732558066 1101 88-89 0.7682205448598118 1101 90-91 0.6448968476310775 1101 92-93 0.6240569805917318 1101 94-95 0.5783860349216035 1101 96-97 0.5337263654759745 1101 98-99 0.6538594220961045 1101 100-101 0.7085055890768714 2201 1 1.4499284573561795 2201 2 0.7667540265011681 2201 3 0.3071478145484754 2201 4 0.21828937434219853 2201 5 0.47113757216683183 2201 6 0.47645549721248415 2201 7 0.5605683606685545 2201 8 0.6472928999534275 2201 9 0.5279775878426989 2201 10-11 0.7816870570475203 2201 12-13 1.0571977657675191 2201 14-15 1.0385058762949555 2201 16-17 0.9812709379694553 2201 18-19 0.8400034274467458 2201 20-21 1.1868207111070745 2201 22-23 0.9928747120582031 2201 24-25 0.9654164761324893 2201 26-27 1.1589810076831384 2201 28-29 1.4144717618797173 2201 30-31 1.3790906578282929 2201 32-33 0.9713458005628652 2201 34-35 0.9458699306007929 2201 36-37 1.1249175869290653 2201 38-39 0.7994521889427588 2201 40-41 0.972048667661582 2201 42-43 0.9329249650978184 2201 44-45 0.9277473213252136 2201 46-47 0.7843923362129033 2201 48-49 0.9345210257812795 2201 50-51 0.8134866451534037 2201 52-53 1.1026608814928593 2201 54-55 1.4095509807755704 2201 56-57 1.0127763342862508 2201 58-59 1.0641006667916102 2201 60-61 0.8789395832639855 2201 62-63 0.7708881496821149 2201 64-65 0.8806998784251263 2201 66-67 0.8959481399157703 2201 68-69 0.9535234192551592 2201 70-71 0.8977324034541816 2201 72-73 0.8617305151501071 2201 74-75 0.8498812914393774 2201 76-77 0.836436755904014 2201 78-79 0.9118840946981344 2201 80-81 1.3751416924105335 2201 82-83 1.2997988092998902 2201 84-85 1.085516559922965 2201 86-87 1.0705098459218263 2201 88-89 1.0283966556619326 2201 90-91 0.9640349507247024 2201 92-93 1.05255435303906 2201 94-95 0.8646528021174369 2201 96-97 0.616521832089397 2201 98-99 0.8825178133733331 2201 100-101 0.965403794060208 2304 1 -1.9404296904356428 2304 2 -0.38597062599846055 2304 3 -0.24641118772055748 2304 4 2.9436326538956337E-4 2304 5 0.3739676253972206 2304 6 0.5009828041198219 2304 7 0.49740843200022056 2304 8 0.5849869117695832 2304 9 0.8120689879213288 2304 10-11 0.8003256096962161 2304 12-13 1.1492191115959756 2304 14-15 1.0735252187715147 2304 16-17 1.0813235376975996 2304 18-19 0.7605758252026789 2304 20-21 1.033817092192109 2304 22-23 0.9209898034837778 2304 24-25 0.9883486157740293 2304 26-27 0.998843416455486 2304 28-29 1.4361020582496664 2304 30-31 1.3340921271319246 2304 32-33 0.7690108825285407 2304 34-35 0.7894808751013755 2304 36-37 0.9861455490518978 2304 38-39 0.8044231316184494 2304 40-41 0.7300202772266786 2304 42-43 0.741431897661478 2304 44-45 0.8326507699619796 2304 46-47 0.7652430831424653 2304 48-49 0.7765358637352051 2304 50-51 0.7465414146203315 2304 52-53 0.9280115351652469 2304 54-55 1.3000653175560615 2304 56-57 0.8732874909301174 2304 58-59 0.6070871237034439 2304 60-61 0.26728257106272224 2304 62-63 0.05238364583039612 2304 64-65 0.17656892120400158 2304 66-67 0.20617798194215098 2304 68-69 0.3583330739889341 2304 70-71 0.4249809411951553 2304 72-73 0.32407581894707604 2304 74-75 0.3785498876572966 2304 76-77 0.31438445406208615 2304 78-79 0.5801208364133394 2304 80-81 1.080967112302627 2304 82-83 0.6807888381626519 2304 84-85 0.27973461531638577 2304 86-87 0.4290682376583028 2304 88-89 0.10350544451293686 2304 90-91 0.03338286169697824 2304 92-93 0.16322391269077485 2304 94-95 0.09318681280645436 2304 96-97 -0.005694709720010849 2304 98-99 0.40529322729427975 2304 100-101 0.21040420331829424 1102 1 1.390587056947691 1102 2 0.18942923563011504 1102 3 0.31566000341922873 1102 4 0.15194984970251824 1102 5 0.26102182289088915 1102 6 0.032175165128954575 1102 7 -4.890236442933102 1102 8 -1.1992390484660405 1102 9 0.09937832980926942 1102 10-11 0.6304983014746313 1102 12-13 0.9164588943397121 1102 14-15 0.8504052812253704 1102 16-17 0.4972515926303487 1102 18-19 0.8309597983398476 1102 20-21 1.0265752000663042 1102 22-23 0.6137040079515259 1102 24-25 0.9651509136884187 1102 26-27 1.0711975423411886 1102 28-29 0.8228653325959883 1102 30-31 1.0797317366842947 1102 32-33 0.8477324153327146 1102 34-35 1.0168191730520988 1102 36-37 1.2034626169300964 1102 38-39 0.7627190335130152 1102 40-41 1.124340589415226 1102 42-43 0.8902504166229619 1102 44-45 1.0889590586728062 1102 46-47 0.9289106929754141 1102 48-49 0.8403650078256817 1102 50-51 0.6680123503129423 1102 52-53 0.7891179615642088 1102 54-55 1.3425531671621727 1102 56-57 1.181869446226358 1102 58-59 0.9765915148010436 1102 60-61 0.8239949225039425 1102 62-63 0.6127624868107446 1102 64-65 0.23867730408005627 1102 66-67 0.3688293540749932 1102 68-69 0.5911496087182933 1102 70-71 0.4155242988368464 1102 72-73 0.28190008723625226 1102 74-75 0.4747556745299768 1102 76-77 0.5453196521295283 1102 78-79 0.6211098136305004 1102 80-81 1.216303500707486 1102 82-83 1.031469265267102 1102 84-85 0.49378789386634026 1102 86-87 0.3886041836984937 1102 88-89 0.30707130946302996 1102 90-91 -0.07152447940675444 1102 92-93 0.25566263589897176 1102 94-95 0.16892165237987555 1102 96-97 -0.027774086069598525 1102 98-99 0.14012891501086244 1102 100-101 0.15493835537446188 2202 1 1.4932938875225688 2202 2 1.0266978845185193 2202 3 0.4528720062132443 2202 4 -0.30046057866072573 2202 5 0.23434779919306692 2202 6 0.4314403974138301 2202 7 0.5818090140349028 2202 8 -4.168317764131146 2202 9 -0.47093352608341377 2202 10-11 0.5565706344475032 2202 12-13 -0.0402605745792286 2202 14-15 0.05778899303684426 2202 16-17 0.24636350630031245 2202 18-19 0.7078807676841734 2202 20-21 1.110839310958653 2202 22-23 -2.4549460986514617 2202 24-25 -3.0836204164267045 2202 26-27 -1.617779560585248 2202 28-29 0.4834145908780343 2202 30-31 1.2246640539555003 2202 32-33 1.0381162078496402 2202 34-35 -1.9500624942573879 2202 36-37 -0.1298244902317407 2202 38-39 0.25814378459323706 2202 40-41 1.2470307220092849 2202 42-43 1.3199558547567847 2202 44-45 1.234459631551026 2202 46-47 0.854128331229191 2202 48-49 0.8703202739437614 2202 50-51 -3.812758921937885 2202 52-53 -0.39611661613414384 2202 54-55 1.6172375620550596 2202 56-57 1.3688255953187038 2202 58-59 1.4713858496695167 2202 60-61 1.4173703205656665 2202 62-63 1.2174088043904092 2202 64-65 1.195485418480807 2202 66-67 1.5123850559590402 2202 68-69 1.391373908979137 2202 70-71 1.38626193583762 2202 72-73 1.272456896155532 2202 74-75 0.6718837023170146 2202 76-77 1.4269046790266344 2202 78-79 1.4205131914693077 2202 80-81 1.8166108122955897 2202 82-83 1.674543072909234 2202 84-85 1.565712281772008 2202 86-87 1.387263189655254 2202 88-89 1.3849330956031416 2202 90-91 1.1047239480876598 2202 92-93 1.063896844005498 2202 94-95 1.0249073324908515 2202 96-97 0.9142403482427959 2202 98-99 1.2028073616287713 2202 100-101 1.2834802862101036 2305 1 1.3538003755139592 2305 2 0.7264173655395538 2305 3 0.11386681898813933 2305 4 -0.14819285539017102 2305 5 0.3072831108678358 2305 6 0.2410076861402004 2305 7 0.449128213556385 2305 8 -0.1715521426786495 2305 9 0.46984181962473315 2305 10-11 0.29066274182464724 2305 12-13 0.847256896571217 2305 14-15 0.6593009971220525 2305 16-17 0.6397891114451397 2305 18-19 0.6510564401490342 2305 20-21 0.7880252507651164 2305 22-23 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42-43 0.3217938737930126 1208 44-45 0.1999618273471171 1208 46-47 -0.08315653542446455 1208 48-49 -1.4972894155168284 1208 50-51 -0.17358399594998986 1208 52-53 -1.4700989584181343 1208 54-55 -0.9173062618272851 1208 56-57 -6.109580449106545 1208 58-59 -1.8486464125471471 1208 60-61 -1.0107140705282518 1208 62-63 0.4163228091819988 1208 64-65 0.07538513018308635 1208 66-67 0.7993073705897054 1208 68-69 0.7508540319381858 1208 70-71 -3.9314454675867943 1208 72-73 -0.4542727171269263 1208 74-75 -5.7209087856106 1208 76-77 -1.2312414268781424 1208 78-79 -3.877140358550971 1208 80-81 1.0249394744585594 1208 82-83 0.5758485093211121 1208 84-85 -2.511910459284607 1208 86-87 -0.6895613002875471 1208 88-89 -4.740709968850705 1208 90-91 0.1410407406337164 1208 92-93 -2.0277677811867107 1208 94-95 -4.389462156393147 1208 96-97 -3.2782796893480146 1208 98-99 -6.315702929478565 1208 100-101 -4.079984518078238 1209 1 1.652281751715897 1209 2 1.2706015751086 1209 3 0.6320428421581035 1209 4 0.4412512628223979 1209 5 0.27922572639288745 1209 6 1.2549727198575624 1209 7 0.9175761062428407 1209 8 0.2143596638548786 1209 9 -0.08906612928908686 1209 10-11 -2.5638225422309304 1209 12-13 -0.2187331709674396 1209 14-15 -1.395372144525517 1209 16-17 -0.1407700095649389 1209 18-19 -16.74068683326734 1209 20-21 -13.826188094485783 1209 22-23 -3.3210435443042314 1209 24-25 -2.1254249899874367 1209 26-27 -1.281047578281317 1209 28-29 -2.652296788777349 1209 30-31 -0.9457329712874198 1209 32-33 -0.5577396212594365 1209 34-35 -3.0905057755801195 1209 36-37 -4.650376740908904 1209 38-39 -1.7843553632246305 1209 40-41 -10.122602821345708 1209 42-43 -4.825179926263559 1209 44-45 -2.118601260069731 1209 46-47 -2.4275270279884893 1209 48-49 -2.3741698645679037 1209 50-51 -2.5356393708637697 1209 52-53 -1.0888481439409432 1209 54-55 -2.450030763526165 1209 56-57 -0.4855685489987991 1209 58-59 -1.535002767376323 1209 60-61 -1.9835804581272214 1209 62-63 -0.8007564199808925 1209 64-65 -0.017528044164301093 1209 66-67 0.3201730843577799 1209 68-69 -0.731782903529794 1209 70-71 -0.49765253073300997 1209 72-73 0.027806400790304053 1209 74-75 0.5056302981700966 1209 76-77 -16.855651427545617 1209 78-79 -16.864763483664206 1209 80-81 -22.146965273824286 1209 82-83 -9.776921030980017 1209 84-85 -3.5601632666484306 1209 86-87 -2.4331829506011005 1209 88-89 -1.6174585123271008 1209 90-91 -5.955949587350286 1209 92-93 -3.473843828723574 1209 94-95 -0.5954088728978739 1209 96-97 0.41884762972680534 1209 98-99 1.0909818225548484 1209 100-101 0.28709518785179 1212 1 -28.59897452969114 1212 2 -16.257036615846175 1212 3 -5.669888894446995 1212 4 -1.3885633948817073 1212 5 -0.904582927265011 1212 6 -2.4889253418796997 1212 7 -0.12393828388963612 1212 8 -1.1148268531457077 1212 9 -0.21061543425548024 1212 10-11 0.5463182591787188 1212 12-13 0.1348284683982186 1212 14-15 -0.25081004833025844 1212 16-17 -0.272720345661007 1212 18-19 -1.3496366146417387 1212 20-21 -0.8669479000068918 1212 22-23 -0.5259928035876626 1212 24-25 0.2279938901274292 1212 26-27 -0.7088603434056324 1212 28-29 0.13960509075015892 1212 30-31 -0.5572605768476819 1212 32-33 -0.03211229632306356 1212 34-35 -1.3654029889195414 1212 36-37 -1.5861881029137344 1212 38-39 -0.6414288801599568 1212 40-41 -0.8271360393839515 1212 42-43 0.29174805781266855 1212 44-45 0.8070456432781796 1212 46-47 -2.5163028878070577 1212 48-49 -1.3189546651944113 1212 50-51 0.1487199256186429 1212 52-53 -0.9996888735630804 1212 54-55 -0.6160964817275598 1212 56-57 -1.3637867503953913 1212 58-59 0.12450010412403145 1212 60-61 0.5234025086547405 1212 62-63 -4.587812642652693 1212 64-65 -4.029152726015113 1212 66-67 -1.5453028341960255 1212 68-69 0.19152274810500458 1212 70-71 -0.47769886630698366 1212 72-73 -0.9878481707687925 1212 74-75 -0.022999049868797528 1212 76-77 1.2332060089877004 1212 78-79 -1.1459339463673324 1212 80-81 0.6951470736272576 1212 82-83 0.7169036117139775 1212 84-85 -2.7169985795767104 1212 86-87 -1.5610830028101965 1212 88-89 -1.0294861831487445 1212 90-91 0.13798599967574887 1212 92-93 -2.9247999078203577 1212 94-95 -1.8863155165055225 1212 96-97 -0.1349440559744295 1212 98-99 -0.6107369988247768 1212 100-101 0.16090824665228354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 6.0 11 8.0 12 43.0 13 155.0 14 508.0 15 1228.0 16 2257.0 17 3704.0 18 5553.0 19 7783.0 20 10730.0 21 14560.0 22 18327.0 23 24086.0 24 31055.0 25 38610.0 26 47510.0 27 57503.0 28 69690.0 29 82881.0 30 98207.0 31 115322.0 32 134311.0 33 154946.0 34 174973.0 35 193338.0 36 207432.0 37 195633.0 38 72385.0 39 178.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.34283672458617 20.713976828771578 16.8669770864074 21.076209360234856 2 23.178644908294793 29.027436400837946 28.264422305129628 19.529496385737634 3 18.5407229167163 26.799265307326394 32.2270400030858 22.432971772871507 4 21.219839119279875 27.632605204657494 29.863731840507885 21.283823835554742 5 20.19112576102303 29.140410556785522 28.873978046687238 21.794485635504216 6 19.999228553941382 29.40446065993807 28.841248313170798 21.755062472949753 7 21.156284193921117 28.301746587911097 30.20625404512846 20.335715173039322 8 20.133153858676756 27.978646826889207 30.532303452845078 21.355895861588962 9 20.645257904060472 27.8307674243288 29.93828998770792 21.585684683902816 10-11 21.323931901733655 28.44905875072252 28.61920231343059 21.60780703411323 12-13 21.228054472220446 28.588169270222846 28.598967102131862 21.584809155424843 14-15 21.09312204787163 28.79635128703863 28.653151612407346 21.457375052682394 16-17 21.39531480265061 28.619717127437866 28.445488549072728 21.539479520838793 18-19 21.548441906879848 28.654562945417407 28.285494189538017 21.51150095816473 20-21 21.328583267675334 28.749468921785017 28.400673200134097 21.521274610405563 22-23 21.313699269253185 28.56727961665927 28.512767861321187 21.606253252766358 24-25 21.401813919269305 28.42500778536555 28.616706458535056 21.55647183683008 26-27 21.415058967408108 28.5903014482198 28.515397439366325 21.479242145005767 28-29 21.544162734277105 28.57490088903486 28.53457014288202 21.346366233806013 30-31 21.598163958380486 28.407990592896006 28.329229353749426 21.664616094974086 32-33 21.409783637742542 28.598895130416928 28.44386850701931 21.54745272482122 34-35 21.708293555645934 28.40872800456968 28.365759593584066 21.51721884620032 36-37 21.65528500110328 28.4802569369167 28.376792406701824 21.487665655278192 38-39 21.407145972909763 28.51514218147928 28.652300752783873 21.425411092827083 40-41 21.65637486584544 28.403413255037748 28.30780331409979 21.632408565017027 42-43 21.401019783620725 28.568207459996835 28.52699749223307 21.50377526414937 44-45 21.35870369834644 28.352571269420164 28.73730162916928 21.551423403064117 46-47 21.58496655079111 28.37948781377605 28.50246493237251 21.533080703060328 48-49 21.553641751126325 28.311766399260318 28.563110403321758 21.5714814462916 50-51 21.59252604694761 28.40295021477121 28.513193291253586 21.491330447027593 52-53 21.487758945386066 28.46447938647244 28.59755405804007 21.450207610101423 54-55 21.29745916350707 28.307761599742243 28.765619292023754 21.629159944726936 56-57 21.42429275464917 28.490336355633005 28.57616104410016 21.509209845617665 58-59 21.42676058297305 28.309401443947596 28.767210789606146 21.496627183473212 60-61 21.573864506577266 28.222038811270128 28.52863763479287 21.675459047359734 62-63 21.432139004930225 28.403373642826896 28.621082913594435 21.543404438648444 64-65 21.514935786894174 28.311910056271554 28.61646850608096 21.556685650753312 66-67 21.608556869280893 28.29943256368555 28.521432165464724 21.57057840156883 68-69 21.496122867889337 28.362443142061107 28.616834289510116 21.524599700539444 70-71 21.771685021798405 28.240922342887988 28.384354161153603 21.60303847416001 72-73 21.45878641114142 28.388951072561962 28.6219767031115 21.530285813185113 74-75 21.480045556974876 28.21201358083611 28.788852955046252 21.519087907142765 76-77 21.60732820402985 28.44627326351981 28.544926097012123 21.40147243543821 78-79 21.63322400701564 28.379822544213628 28.46575960991946 21.521193838851268 80-81 21.519371207726508 28.275520550718543 28.605096429410082 21.600011812144874 82-83 21.535201496224552 28.371284469295798 28.543705402144386 21.549808632335264 84-85 21.483169000362203 28.10055515736455 28.6445656949451 21.771710147328154 86-87 21.460199142560942 28.336701540015003 28.603236598437437 21.599862718986618 88-89 21.561347358006984 28.29283332520232 28.496118103151918 21.64970121363877 90-91 21.67463578685783 28.124247354304106 28.40852580023347 21.792591058604593 92-93 21.448149393058788 28.326617907141326 28.542674512440204 21.68255818735968 94-95 21.651359335278496 28.240764116566808 28.39924591371124 21.708630634443452 96-97 21.636884982480446 28.269808812337804 28.28072928322851 21.812576921953237 98-99 21.512950528391542 28.298844732082685 28.459166237402904 21.72903850212287 100-101 21.708849377286636 28.121381593690092 28.22312954809385 21.94663948092942 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 3.5 2 3.0 3 4.5 4 9.5 5 12.5 6 14.0 7 29.5 8 46.5 9 58.0 10 95.5 11 146.0 12 218.5 13 330.5 14 491.5 15 722.5 16 1108.5 17 1641.5 18 2454.5 19 3654.0 20 5177.0 21 7107.5 22 9492.0 23 12314.0 24 15595.0 25 19405.0 26 23570.5 27 27909.5 28 32259.0 29 36543.0 30 40864.0 31 44886.5 32 48584.0 33 52184.0 34 55800.0 35 59243.0 36 61784.0 37 63727.0 38 64674.5 39 64262.5 40 62955.0 41 60612.0 42 57414.0 43 54031.0 44 50635.5 45 47520.0 46 44698.0 47 42502.0 48 40521.5 49 38094.5 50 35615.5 51 33026.0 52 30816.0 53 28763.0 54 26831.0 55 25234.0 56 23755.0 57 22628.0 58 21627.0 59 20557.0 60 19635.5 61 18851.5 62 18084.0 63 17373.0 64 16899.5 65 16595.0 66 16252.5 67 15834.5 68 15274.5 69 14387.5 70 13328.0 71 12013.0 72 10503.5 73 8928.5 74 7125.5 75 5485.5 76 4076.0 77 2802.5 78 1875.5 79 1171.5 80 718.0 81 473.0 82 301.5 83 205.5 84 151.0 85 102.5 86 69.5 87 49.5 88 32.0 89 20.5 90 10.5 91 7.0 92 7.0 93 5.5 94 4.0 95 3.0 96 1.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7028100489924971 2 0.002609302845331305 3 5.105157740865597E-4 4 1.1344794979701326E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.1877847189015062 14-15 0.0 16-17 5.105157740865597E-4 18-19 0.11370320768405655 20-21 0.0 22-23 2.8361987449253314E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0017584432218537053 42-43 0.0 44-45 0.0 46-47 0.02268958995940265 48-49 2.8361987449253314E-5 50-51 2.8361987449253314E-5 52-53 1.7017192469551988E-4 54-55 0.0022122350210417585 56-57 0.0015315473222596789 58-59 0.09696963508899707 60-61 0.0018718911716507187 62-63 0.0012195654603178925 64-65 0.002439130920635785 66-67 0.004651365941677543 68-69 0.0049066238287208234 70-71 0.003658696380953678 72-73 0.038033425169448694 74-75 0.041635397575503864 76-77 0.15372197197495296 78-79 2.8361987449253317E-4 80-81 0.11478096320712816 82-83 0.00487826184127157 84-85 0.0050767957534163435 86-87 0.006551619100777515 88-89 0.007090496862313329 90-91 0.022150712197866837 92-93 0.010805917218165513 94-95 0.014748233473611724 96-97 0.010125229519383433 98-99 0.012195654603178925 100-101 0.013273410126250551 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1762923.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.00365044194055 #Duplication Level Percentage of deduplicated Percentage of total 1 98.60439214148154 96.63590379473787 2 1.0417140941357015 2.041835678842361 3 0.20070274059487894 0.5900880369599997 4 0.0738294382914453 0.28942217850558505 5 0.04890233403319811 0.23963036251922806 6 0.02149702018283195 0.1264071870924962 7 0.00554032835314454 0.03800806822786047 8 0.0016195871767084706 0.012698036442110908 9 0.0 0.0 >10 0.0018023157505622528 0.026006656672495722 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.1344794979701326E-4 0.0 0.0 2 1.1344794979701326E-4 0.0 0.0 3 1.1344794979701326E-4 0.0 0.0 4 1.1344794979701326E-4 0.0 0.0 5 1.1344794979701326E-4 0.0 5.672397489850663E-5 6 2.268958995940265E-4 0.0 5.672397489850663E-5 7 2.268958995940265E-4 0.0 1.1344794979701326E-4 8 2.268958995940265E-4 0.0 1.1344794979701326E-4 9 2.268958995940265E-4 0.0 1.1344794979701326E-4 10-11 2.268958995940265E-4 2.8361987449253314E-5 1.7017192469551988E-4 12-13 2.8361987449253317E-4 5.672397489850663E-5 1.7017192469551988E-4 14-15 2.8361987449253317E-4 5.672397489850663E-5 1.985339121447732E-4 16-17 3.4034384939103977E-4 5.672397489850663E-5 2.268958995940265E-4 18-19 4.53791799188053E-4 5.672397489850663E-5 2.268958995940265E-4 20-21 5.105157740865596E-4 5.672397489850663E-5 2.552578870432798E-4 22-23 5.105157740865596E-4 5.672397489850663E-5 2.8361987449253317E-4 24-25 5.105157740865596E-4 5.672397489850663E-5 2.8361987449253317E-4 26-27 5.105157740865596E-4 5.672397489850663E-5 2.8361987449253317E-4 28-29 5.105157740865596E-4 5.672397489850663E-5 2.8361987449253317E-4 30-31 6.239637238835729E-4 5.672397489850663E-5 2.8361987449253317E-4 32-33 6.806876987820795E-4 5.672397489850663E-5 2.8361987449253317E-4 34-35 9.07583598376106E-4 5.672397489850663E-5 3.1198186194178647E-4 36-37 0.0011344794979701324 5.672397489850663E-5 3.687058368402931E-4 38-39 0.0014748233473611725 5.672397489850663E-5 4.254298117387997E-4 40-41 0.002240597008491012 5.672397489850663E-5 5.105157740865596E-4 42-43 0.0030347326570701045 5.672397489850663E-5 5.105157740865596E-4 44-45 0.004055764205243223 5.672397489850663E-5 5.105157740865596E-4 46-47 0.005644035502401409 5.672397489850663E-5 5.672397489850662E-4 48-49 0.008196614372834208 5.672397489850663E-5 6.239637238835729E-4 50-51 0.011514966904396845 5.672397489850663E-5 6.239637238835729E-4 52-53 0.01662012464526244 5.672397489850663E-5 6.239637238835729E-4 54-55 0.022547780022156384 5.672397489850663E-5 6.239637238835729E-4 56-57 0.029723362846817473 5.672397489850663E-5 6.239637238835729E-4 58-59 0.03868575088078152 1.1344794979701326E-4 6.239637238835729E-4 60-61 0.05201588498193058 1.1344794979701326E-4 6.523257113328262E-4 62-63 0.06787023596606317 1.1344794979701326E-4 7.090496862313328E-4 64-65 0.08568156408419426 1.1344794979701326E-4 7.657736611298394E-4 66-67 0.10553495529867159 1.7017192469551988E-4 8.224976360283461E-4 68-69 0.13069203816615926 1.7017192469551988E-4 8.508596234775994E-4 70-71 0.1605004869753245 1.7017192469551988E-4 8.508596234775994E-4 72-73 0.19873244605691798 1.7017192469551988E-4 8.508596234775994E-4 74-75 0.2426368026283621 2.268958995940265E-4 8.508596234775994E-4 76-77 0.29224191867710614 2.268958995940265E-4 8.508596234775994E-4 78-79 0.35151847244604556 2.8361987449253317E-4 8.508596234775994E-4 80-81 0.42299068081816393 2.8361987449253317E-4 8.508596234775994E-4 82-83 0.5044746707598687 2.8361987449253317E-4 8.508596234775994E-4 84-85 0.5959137182962614 2.8361987449253317E-4 8.508596234775994E-4 86-87 0.7051357319633358 2.8361987449253317E-4 8.508596234775994E-4 88-89 0.8257025406101117 3.4034384939103977E-4 8.792216109268527E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGGTC 245 0.0023834573 8.739192 74-75 GTCGACG 825 0.004906713 6.3713694 1 GTTAGAA 1070 2.7585635E-4 6.2522783 1 CGGAAGA 2375 0.0 6.1952953 94-95 GTTCACA 930 0.0025000598 6.165841 1 CGTCTGA 985 8.800271E-9 6.0252247 90-91 ATCGGAA 2400 0.0 5.933162 92-93 CACGTCT 1240 3.8198777E-11 5.9329944 94-95 TAGATCG 730 1.3688163E-5 5.8517203 94-95 TCGGAAG 2355 0.0 5.844984 92-93 GTAATTA 1085 0.0022053486 5.7254243 1 ACGTCTG 1130 5.187758E-9 5.670472 94-95 GAGCACA 2240 0.0 5.509209 94-95 CACACGT 1295 7.1304385E-10 5.497911 92-93 AGAGCAC 2335 0.0 5.3867006 94-95 AGATCGG 2910 0.0 5.3009567 94-95 GATCGGA 2600 0.0 5.295709 90-91 ACACGTC 1220 3.0828232E-8 5.2521586 94-95 CAGATCG 1855 0.0 5.2453423 94-95 GGTTTAA 1190 0.0065627205 5.2202396 1 >>END_MODULE