FastQCFastQC Report
Thu 11 Sep 2014
LONLib30_CACCGG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib30_CACCGG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762923
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC2450.00238345738.73919274-75
GTCGACG8250.0049067136.37136941
GTTAGAA10702.7585635E-46.25227831
CGGAAGA23750.06.195295394-95
GTTCACA9300.00250005986.1658411
CGTCTGA9858.800271E-96.025224790-91
ATCGGAA24000.05.93316292-93
CACGTCT12403.8198777E-115.932994494-95
TAGATCG7301.3688163E-55.851720394-95
TCGGAAG23550.05.84498492-93
GTAATTA10850.00220534865.72542431
ACGTCTG11305.187758E-95.67047294-95
GAGCACA22400.05.50920994-95
CACACGT12957.1304385E-105.49791192-93
AGAGCAC23350.05.386700694-95
AGATCGG29100.05.300956794-95
GATCGGA26000.05.29570990-91
ACACGTC12203.0828232E-85.252158694-95
CAGATCG18550.05.245342394-95
GGTTTAA11900.00656272055.22023961
Produced by FastQC (version 0.11.2)