##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib29_CAACTA_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2247541 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.836022568665044 27.0 22.0 33.0 2.0 33.0 2 24.85771605501301 33.0 15.0 33.0 2.0 33.0 3 25.732134808664224 33.0 22.0 33.0 6.0 33.0 4 27.88205153988292 33.0 22.0 37.0 2.0 37.0 5 27.956644172453363 33.0 15.0 37.0 6.0 37.0 6 29.02138826388484 33.0 27.0 37.0 15.0 37.0 7 28.688875531080413 33.0 27.0 37.0 6.0 37.0 8 28.46187900465442 33.0 27.0 37.0 2.0 37.0 9 29.227506861943787 33.0 27.0 37.0 15.0 37.0 10-11 28.866134811333808 33.0 24.5 37.0 10.5 37.0 12-13 29.168695031592307 33.0 27.0 37.0 10.5 37.0 14-15 29.524193774440597 33.0 27.0 37.0 6.0 40.0 16-17 30.41116647037807 33.0 27.0 37.0 15.0 40.0 18-19 30.737412354212893 33.0 27.0 37.0 15.0 40.0 20-21 30.766944629708647 33.0 27.0 37.0 15.0 40.0 22-23 30.81794036237826 33.0 27.0 37.0 15.0 40.0 24-25 30.424663443292026 33.0 27.0 37.0 10.5 40.0 26-27 29.912997805156834 33.0 27.0 37.0 15.0 40.0 28-29 30.13359711791687 33.0 27.0 37.0 15.0 40.0 30-31 30.06811466398166 33.0 27.0 37.0 15.0 40.0 32-33 29.91188814798039 33.0 27.0 37.0 15.0 40.0 34-35 29.960764898170936 33.0 27.0 37.0 15.0 40.0 36-37 30.192887471240795 33.0 27.0 37.0 15.0 40.0 38-39 30.259328973309053 33.0 27.0 37.0 15.0 40.0 40-41 30.35679972022757 33.0 27.0 37.0 15.0 40.0 42-43 30.29004810145844 33.0 27.0 37.0 15.0 40.0 44-45 30.06225003236871 33.0 27.0 37.0 15.0 40.0 46-47 29.702010330401095 33.0 27.0 37.0 15.0 40.0 48-49 29.573235593922426 33.0 27.0 37.0 10.5 40.0 50-51 28.82636490279821 33.0 24.5 37.0 15.0 38.5 52-53 28.560470532016993 33.0 22.0 37.0 10.5 38.5 54-55 29.51535967530737 33.0 27.0 37.0 15.0 40.0 56-57 29.457114686673123 33.0 27.0 37.0 15.0 40.0 58-59 29.66626393022419 33.0 27.0 37.0 15.0 40.0 60-61 29.424105500188872 33.0 27.0 37.0 10.5 40.0 62-63 29.315250978736316 33.0 27.0 37.0 15.0 40.0 64-65 29.29556057041896 33.0 27.0 37.0 15.0 40.0 66-67 29.182654954904045 33.0 27.0 37.0 15.0 40.0 68-69 28.90082294383061 33.0 27.0 37.0 15.0 40.0 70-71 28.809869541868203 33.0 27.0 37.0 15.0 38.5 72-73 28.584329051171927 33.0 22.0 37.0 15.0 37.0 74-75 28.544144912150657 33.0 27.0 37.0 15.0 37.0 76-77 28.147265166686616 33.0 22.0 37.0 15.0 37.0 78-79 27.589746972357787 33.0 22.0 37.0 10.5 37.0 80-81 27.476771502722308 30.0 22.0 35.0 10.5 37.0 82-83 27.06775271285374 27.0 22.0 33.0 6.0 37.0 84-85 27.17353876970431 27.0 22.0 33.0 15.0 37.0 86-87 26.879758144567774 27.0 22.0 33.0 6.0 37.0 88-89 26.53498757086078 27.0 22.0 33.0 6.0 37.0 90-91 26.421326240544666 27.0 22.0 33.0 6.0 37.0 92-93 26.323147163944952 27.0 22.0 33.0 6.0 37.0 94-95 26.12691804064976 27.0 22.0 33.0 6.0 37.0 96-97 26.023721925428724 27.0 22.0 33.0 6.0 37.0 98-99 25.815139968525603 27.0 22.0 33.0 6.0 37.0 100-101 25.358558753766893 27.0 18.5 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.134676857028058 2306 2 8.449925601549864 2306 3 7.4295958342496355 2306 4 8.96713900910337 2306 5 8.81855893065535 2306 6 7.832676294951071 2306 7 8.091880455942562 2306 8 8.562317090350419 2306 9 7.365928538743578 2306 10-11 7.746053040404771 2306 12-13 7.387235146276783 2306 14-15 8.040763645396197 2306 16-17 6.7250235864351815 2306 18-19 6.087572036097619 2306 20-21 6.117934118229201 2306 22-23 5.745768789276731 2306 24-25 5.868786012511411 2306 26-27 6.518794399331053 2306 28-29 5.482065431692579 2306 30-31 5.409745117507121 2306 32-33 5.512957808173063 2306 34-35 4.838408836520966 2306 36-37 4.924386470761 2306 38-39 4.685007834367628 2306 40-41 3.820286031358478 2306 42-43 3.7364711945730207 2306 44-45 3.3178743963046387 2306 46-47 3.6678380747915007 2306 48-49 3.4875955527131204 2306 50-51 3.4575670237317375 2306 52-53 3.9629488320692694 2306 54-55 3.6249928036438632 2306 56-57 3.819066938394961 2306 58-59 3.3856287826846874 2306 60-61 3.3570387413114524 2306 62-63 2.8674544778647437 2306 64-65 2.395907729756402 2306 66-67 2.4206393881234796 2306 68-69 2.564302407723922 2306 70-71 2.65908516207778 2306 72-73 1.8220364954094919 2306 74-75 1.4295533727680905 2306 76-77 1.8201157870974782 2306 78-79 2.0381531999810534 2306 80-81 1.5885972916142848 2306 82-83 1.6753024886900967 2306 84-85 1.3129814222341665 2306 86-87 1.2173119478328651 2306 88-89 1.2672092971508135 2306 90-91 1.1730981061295793 2306 92-93 1.1501609776276638 2306 94-95 1.4523342174063956 2306 96-97 1.1785558771031575 2306 98-99 0.9904569061987729 2306 100-101 0.7377843681684482 2307 1 8.206037857568624 2307 2 8.304067696984532 2307 3 7.382402176084614 2307 4 8.91021821446973 2307 5 8.91032633937322 2307 6 7.471595897879965 2307 7 8.038811201925167 2307 8 8.431492172664043 2307 9 7.3662556432696 2307 10-11 7.603559091070647 2307 12-13 7.293084328054764 2307 14-15 8.063848317861684 2307 16-17 6.719806806740845 2307 18-19 6.3486498224753305 2307 20-21 6.233218499525577 2307 22-23 5.899789717823587 2307 24-25 6.0408798500464265 2307 26-27 6.779878328516762 2307 28-29 6.068652917564126 2307 30-31 5.866640265917361 2307 32-33 5.951637227309135 2307 34-35 5.599009019576645 2307 36-37 5.48132569381864 2307 38-39 5.032795194925885 2307 40-41 4.455032364102095 2307 42-43 4.279691991663789 2307 44-45 4.341144120861131 2307 46-47 4.633375386207295 2307 48-49 4.519591903885168 2307 50-51 4.284194714281643 2307 52-53 4.9175066420353595 2307 54-55 4.404569656314756 2307 56-57 4.858865423578507 2307 58-59 4.351070112725473 2307 60-61 4.317970482428951 2307 62-63 3.9775527935067885 2307 64-65 3.9601553586325124 2307 66-67 3.5979515410548295 2307 68-69 3.6622049459321886 2307 70-71 3.4359845183115 2307 72-73 2.7429040748974245 2307 74-75 2.4647101986563413 2307 76-77 2.6491153693865357 2307 78-79 3.075801702364462 2307 80-81 2.8221038521332247 2307 82-83 2.9142047381863883 2307 84-85 2.2944071065429767 2307 86-87 2.3020995909602924 2307 88-89 2.501348566893796 2307 90-91 2.390170351694472 2307 92-93 2.1720339812150655 2307 94-95 2.36835972848802 2307 96-97 2.178925981285179 2307 98-99 2.078224579057391 2307 100-101 1.8706571444719486 1101 1 8.070693740755463 1101 2 7.822170898107636 1101 3 7.27022700779759 1101 4 8.801081296381781 1101 5 8.58797049546407 1101 6 7.546011882297165 1101 7 7.189906704162443 1101 8 8.122045455963693 1101 9 7.3665315474715065 1101 10-11 7.482964967957908 1101 12-13 7.319439853372305 1101 14-15 7.820585371597758 1101 16-17 6.906778137197346 1101 18-19 5.984209072318983 1101 20-21 5.908503483059427 1101 22-23 5.468221647151232 1101 24-25 5.9552543708594605 1101 26-27 6.483146597269215 1101 28-29 6.00852108795495 1101 30-31 5.790399428289298 1101 32-33 5.819858102917969 1101 34-35 5.540596646275393 1101 36-37 5.478568810398677 1101 38-39 5.109894582289041 1101 40-41 4.579344409772062 1101 42-43 4.409815977188309 1101 44-45 4.342361130198789 1101 46-47 4.735898532997144 1101 48-49 4.368180739575603 1101 50-51 3.8699750839641993 1101 52-53 4.757398614284764 1101 54-55 4.554247972191625 1101 56-57 4.744342602604959 1101 58-59 4.17544521591973 1101 60-61 4.351670641037526 1101 62-63 4.073113863622137 1101 64-65 3.7698935963381466 1101 66-67 3.4672929718659162 1101 68-69 3.658155458154525 1101 70-71 3.582589517767886 1101 72-73 3.114362628826367 1101 74-75 2.565366818057619 1101 76-77 2.837981509284546 1101 78-79 3.4266426176668503 1101 80-81 2.9485328006558404 1101 82-83 3.106354922231187 1101 84-85 2.357462890733821 1101 86-87 2.3766954219213723 1101 88-89 2.53416367786253 1101 90-91 2.5073347031975715 1101 92-93 2.2235931549350667 1101 94-95 2.424173985532587 1101 96-97 2.3630363530100347 1101 98-99 2.2943482963869926 1101 100-101 1.9215413154562562 2201 1 7.169527890641891 2201 2 7.7272107054543255 2201 3 6.2983140302248835 2201 4 8.293713478040651 2201 5 4.017325939189824 2201 6 5.717337658457836 2201 7 6.788163984241013 2201 8 7.69885181953083 2201 9 6.473686056305379 2201 10-11 4.328833579396434 2201 12-13 6.34745703450584 2201 14-15 7.012959731973751 2201 16-17 5.9678258468629615 2201 18-19 1.9194038002740008 2201 20-21 4.924908168928269 2201 22-23 4.974859637117042 2201 24-25 5.460366499435441 2201 26-27 6.218156460489819 2201 28-29 5.072233830100277 2201 30-31 5.238580277280768 2201 32-33 5.354674404103768 2201 34-35 4.960159827978348 2201 36-37 4.875684478885454 2201 38-39 4.468199763680548 2201 40-41 4.18153286962432 2201 42-43 3.9737078355249267 2201 44-45 3.8317057104048473 2201 46-47 4.165294177091166 2201 48-49 3.928314904087518 2201 50-51 3.6067985424367137 2201 52-53 4.489081464356364 2201 54-55 4.057033890574409 2201 56-57 4.084372455595609 2201 58-59 3.7881290389497764 2201 60-61 3.7850961365448548 2201 62-63 3.469834034970784 2201 64-65 3.486580087883979 2201 66-67 3.1268754208276626 2201 68-69 3.2863824961642294 2201 70-71 3.190727281603891 2201 72-73 2.5318034282524238 2201 74-75 2.085574261597241 2201 76-77 2.476600923043563 2201 78-79 2.785843971518858 2201 80-81 2.18708590461938 2201 82-83 2.6506114748035827 2201 84-85 2.2276331834371916 2201 86-87 2.0811388748784374 2201 88-89 2.174741386291693 2201 90-91 1.914998729244079 2201 92-93 1.8859137418963705 2201 94-95 2.0161224586064925 2201 96-97 1.8853029974303084 2201 98-99 1.834247764357574 2201 100-101 1.5554515463470153 2304 1 1.6344010895181071 2304 2 4.826854685486278 2304 3 5.710392235354767 2304 4 4.289961605544683 2304 5 6.551407222443146 2304 6 6.3953116014395945 2304 7 6.961441779420429 2304 8 6.57124151974363 2304 9 5.806912358664821 2304 10-11 6.704992353511717 2304 12-13 6.485895483177057 2304 14-15 6.790987867577186 2304 16-17 5.322611136176423 2304 18-19 5.119853264835172 2304 20-21 4.852097782380454 2304 22-23 4.5252304885200445 2304 24-25 4.807773095251971 2304 26-27 5.663331045482682 2304 28-29 4.9434032133878 2304 30-31 4.862890091322093 2304 32-33 5.020760482344727 2304 34-35 4.5792250696914145 2304 36-37 4.838084770796051 2304 38-39 4.433860950798209 2304 40-41 3.7087383957427136 2304 42-43 3.3725457085005743 2304 44-45 3.039331492550076 2304 46-47 4.047842481917133 2304 48-49 3.78706296060162 2304 50-51 3.3836184840109063 2304 52-53 4.089408531353822 2304 54-55 3.857054707006675 2304 56-57 4.463579100515325 2304 58-59 3.8198211833568365 2304 60-61 3.7696204406578566 2304 62-63 3.5941666203978855 2304 64-65 3.432600272178206 2304 66-67 3.21173132437222 2304 68-69 3.3542287752215003 2304 70-71 3.4015131532532195 2304 72-73 2.99118217024094 2304 74-75 2.4053277124724275 2304 76-77 2.5932712375005664 2304 78-79 3.0060452924121357 2304 80-81 2.8146802589757804 2304 82-83 2.8561682826943446 2304 84-85 2.4774580567365447 2304 86-87 2.342316458049151 2304 88-89 2.623774110247453 2304 90-91 2.434127840052593 2304 92-93 2.2724516262740835 2304 94-95 2.65786767290216 2304 96-97 2.4627671562520383 2304 98-99 2.202954779953952 2304 100-101 1.865595199388423 1102 1 7.095703688813401 1102 2 7.481536368663729 1102 3 6.092393821865734 1102 4 6.211153886984349 1102 5 6.571880224085 1102 6 5.72135362553243 1102 7 6.871576448374622 1102 8 6.487465439163628 1102 9 5.0380935714233175 1102 10-11 6.0350586733597105 1102 12-13 6.027921420786594 1102 14-15 6.252733029641128 1102 16-17 5.270901209414202 1102 18-19 5.385696606101057 1102 20-21 5.568932785239866 1102 22-23 5.127429650989832 1102 24-25 1.8461054139561988 1102 26-27 5.464882505626203 1102 28-29 4.971618665266721 1102 30-31 5.000775604263232 1102 32-33 5.157585431152079 1102 34-35 5.06883775808959 1102 36-37 4.889086763158659 1102 38-39 3.9565128223277206 1102 40-41 3.8776722972683473 1102 42-43 4.231275116141653 1102 44-45 -3.6022978173176554 1102 46-47 1.4696995298998594 1102 48-49 -0.6256169515868457 1102 50-51 2.6620435029199037 1102 52-53 -0.14999126940992014 1102 54-55 2.706140249464294 1102 56-57 -0.6755664895376157 1102 58-59 2.830293170369586 1102 60-61 3.807866054692944 1102 62-63 3.67724786451765 1102 64-65 3.198463629309206 1102 66-67 3.238406845983821 1102 68-69 3.4709351046623453 1102 70-71 3.2337790045270403 1102 72-73 2.7136225377211503 1102 74-75 2.607366840260351 1102 76-77 2.8709198806647294 1102 78-79 -1.3195658832662858 1102 80-81 1.6522918220580394 1102 82-83 2.7781604608263173 1102 84-85 2.3650879753817406 1102 86-87 2.273081211678754 1102 88-89 2.349101282014935 1102 90-91 2.0945813792633956 1102 92-93 2.137251190905964 1102 94-95 2.334579444444582 1102 96-97 2.378278529321939 1102 98-99 2.239247970870256 1102 100-101 1.967666334112721 2202 1 -20.630846018788937 2202 2 -7.7458113910603 2202 3 -0.9276165752337064 2202 4 1.2567845972922598 2202 5 0.9890984851119882 2202 6 1.4365746137219197 2202 7 3.724107112344022 2202 8 2.477863900525783 2202 9 3.034013922333731 2202 10-11 1.839710893705135 2202 12-13 1.2273849570294537 2202 14-15 2.539802326474998 2202 16-17 1.3920540251845708 2202 18-19 1.419565805275269 2202 20-21 1.0186915426369971 2202 22-23 1.1485683492929155 2202 24-25 2.1138665331225717 2202 26-27 3.216298653683559 2202 28-29 -1.2475181429566256 2202 30-31 0.8758340588822122 2202 32-33 1.296147442542381 2202 34-35 1.6179519617564786 2202 36-37 2.746683837355082 2202 38-39 2.2412026449625024 2202 40-41 1.5923388509952758 2202 42-43 0.3335806480296881 2202 44-45 0.49392584322535527 2202 46-47 -1.5649401508128555 2202 48-49 0.6776575036034878 2202 50-51 1.6640224728762725 2202 52-53 1.439992238066523 2202 54-55 -0.8569207164029926 2202 56-57 0.9794757216895889 2202 58-59 1.5558784390415994 2202 60-61 1.8103759078546737 2202 62-63 -2.2519895431167463 2202 64-65 -0.14969493808342804 2202 66-67 0.20871532094856704 2202 68-69 1.2132956448464718 2202 70-71 -2.735286934010489 2202 72-73 -4.6483943334827025 2202 74-75 -1.470674983982164 2202 76-77 -0.4395622557565062 2202 78-79 1.1921443731567507 2202 80-81 1.3466335743845121 2202 82-83 -1.1637243041012404 2202 84-85 -0.6397874338172045 2202 86-87 0.17852337905877036 2202 88-89 0.8956867981578434 2202 90-91 0.9187607142808574 2202 92-93 0.9575794801512743 2202 94-95 1.311706955070047 2202 96-97 0.9478161905724818 2202 98-99 0.9265637420742756 2202 100-101 0.6276315885343955 2305 1 5.421189570318639 2305 2 6.829833840121648 2305 3 6.6872589752227505 2305 4 7.510631465625842 2305 5 7.549720501961318 2305 6 5.9586450566806235 2305 7 2.834592220031677 2305 8 5.99811994006944 2305 9 6.089346679478798 2305 10-11 4.506481023609375 2305 12-13 5.001729874697499 2305 14-15 5.464514960427472 2305 16-17 4.82300393409685 2305 18-19 4.288647008078861 2305 20-21 4.973592227500589 2305 22-23 4.82570140658985 2305 24-25 5.010356177469635 2305 26-27 5.832279654019789 2305 28-29 4.972697596079858 2305 30-31 5.110291543838059 2305 32-33 5.021488880798032 2305 34-35 5.002333459236954 2305 36-37 4.5410742946728035 2305 38-39 4.369055601942346 2305 40-41 3.708605431307724 2305 42-43 3.797370799314713 2305 44-45 2.7807141072092207 2305 46-47 3.6092400501138506 2305 48-49 3.243752134830107 2305 50-51 2.1974151530391843 2305 52-53 3.821378419303638 2305 54-55 3.881057339078776 2305 56-57 4.436104473616652 2305 58-59 4.188810921564325 2305 60-61 4.168784635858717 2305 62-63 3.502508657373337 2305 64-65 3.9696838475550074 2305 66-67 3.4758993369848454 2305 68-69 3.812025608399896 2305 70-71 3.4483156066596763 2305 72-73 -1.153995820657947 2305 74-75 1.8062191341117213 2305 76-77 2.749710841170156 2305 78-79 3.3849943219560394 2305 80-81 3.0613248206595145 2305 82-83 3.2702780064203303 2305 84-85 2.647308463241991 2305 86-87 2.5445511465140456 2305 88-89 2.64118656847635 2305 90-91 2.489728697545619 2305 92-93 2.4807796084268006 2305 94-95 2.7192801960539903 2305 96-97 2.696934430330863 2305 98-99 2.68928740395463 2305 100-101 2.1941512396451763 1103 1 -20.630846018788937 1103 2 -7.747076144353976 1103 3 -0.6660959241888982 1103 4 2.798085714269458 1103 5 4.667885190839787 1103 6 4.474262381440461 1103 7 3.9146516306224797 1103 8 4.394534052477734 1103 9 4.353805139155433 1103 10-11 4.890799380721717 1103 12-13 4.54032629141064 1103 14-15 4.106495246255722 1103 16-17 2.926017968206157 1103 18-19 3.1603837806584565 1103 20-21 2.533407470812236 1103 22-23 2.5931798712894434 1103 24-25 2.981872457134923 1103 26-27 4.130178307917323 1103 28-29 3.8437752982876248 1103 30-31 3.5802239540188943 1103 32-33 2.956722357371735 1103 34-35 2.3855748204944653 1103 36-37 2.705113259193265 1103 38-39 2.3852355852853435 1103 40-41 2.4728637576544727 1103 42-43 2.2992621785465204 1103 44-45 2.5666279216873917 1103 46-47 1.9256274919282212 1103 48-49 -1.1338678728397475 1103 50-51 -1.9407354764920939 1103 52-53 1.704008069691259 1103 54-55 2.3552604529410246 1103 56-57 3.111459666033962 1103 58-59 2.732401938293961 1103 60-61 3.1720177009318284 1103 62-63 2.719924204339808 1103 64-65 2.654505746588704 1103 66-67 2.563764332165025 1103 68-69 2.6761857455339317 1103 70-71 2.7380145648891343 1103 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1209 48-49 -19.9711635865358 1209 50-51 -21.79175964902776 1209 52-53 -24.772447409760797 1209 54-55 -21.876847162568613 1209 56-57 -25.69533257140896 1209 58-59 -25.975788700975443 1209 60-61 -25.730379559342143 1209 62-63 -25.874681960043755 1209 64-65 -25.762589002269742 1209 66-67 -13.632839065404822 1209 68-69 -25.461228638027713 1209 70-71 -25.320490001320913 1209 72-73 -15.752072774004489 1209 74-75 -15.997709976074272 1209 76-77 -13.638099843787643 1209 78-79 -14.526567568269751 1209 80-81 -24.388526891392253 1209 82-83 -15.940826554735184 1209 84-85 -13.50613414772263 1209 86-87 -13.53603564826772 1209 88-89 -10.828282006715366 1209 90-91 -13.383033845238538 1209 92-93 -17.816795790955474 1209 94-95 -23.17664862573086 1209 96-97 -18.678169086893742 1209 98-99 -10.302177343828923 1209 100-101 -5.484751150276217 1212 1 -20.630846018788937 1212 2 -20.426299888646213 1212 3 -21.3022260611752 1212 4 -23.31492798436068 1212 5 -23.50905544842962 1212 6 -24.566833508970497 1212 7 -24.138715949286198 1212 8 -23.75814859592409 1212 9 -24.704128650798904 1212 10-11 -24.455233724301113 1212 12-13 -24.716523023657853 1212 14-15 -25.089138315388112 1212 16-17 -26.108513995264165 1212 18-19 -20.794219727637575 1212 20-21 -26.543932711836163 1212 22-23 -26.758418302226538 1212 24-25 -22.92159854064979 1212 26-27 -25.532778313087245 1212 28-29 -20.368289272895524 1212 30-31 -15.311790547088751 1212 32-33 -25.712940884637398 1212 34-35 -21.877636389325147 1212 36-37 -26.155474640350114 1212 38-39 -26.446528113344794 1212 40-41 -21.03850655745923 1212 42-43 -17.179811242494214 1212 44-45 -14.622807631456961 1212 46-47 -17.766476864055324 1212 48-49 -20.388073815694305 1212 50-51 -12.555724287016984 1212 52-53 -5.28234839985981 1212 54-55 -4.105618768542769 1212 56-57 -4.176768214973315 1212 58-59 -3.170095631668513 1212 60-61 -14.390033024688679 1212 62-63 -12.448320573905141 1212 64-65 -16.575089002269742 1212 66-67 -12.837522457472842 1212 68-69 -8.053431608324743 1212 70-71 -15.67692564488527 1212 72-73 -12.047876506920154 1212 74-75 -8.687165468233658 1212 76-77 -19.488808855366766 1212 78-79 -10.163062785588092 1212 80-81 -8.30003679238235 1212 82-83 -5.602545057093895 1212 84-85 -5.077276537385327 1212 86-87 -3.6276675868853125 1212 88-89 -3.600130377993171 1212 90-91 -3.648755862355504 1212 92-93 -3.4348932998589063 1212 94-95 -3.4978119920674935 1212 96-97 -13.496428349260658 1212 98-99 -10.715689351809381 1212 100-101 -6.971069697385168 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 413.0 3 843.0 4 353.0 5 2326.0 6 3043.0 7 2671.0 8 5431.0 9 10556.0 10 13703.0 11 16311.0 12 21174.0 13 26896.0 14 31188.0 15 33682.0 16 35320.0 17 38728.0 18 43828.0 19 48455.0 20 52875.0 21 59288.0 22 66095.0 23 72456.0 24 76384.0 25 79136.0 26 83816.0 27 88069.0 28 90081.0 29 91377.0 30 93799.0 31 100617.0 32 109823.0 33 128326.0 34 148389.0 35 165653.0 36 177195.0 37 167525.0 38 61550.0 39 166.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.96082960607649 26.925135691076008 15.99097715641058 20.123057546436932 2 25.68654151752557 31.663821153887568 23.72421447069706 18.9254228578898 3 18.326509178061098 28.128171060580335 31.060649433206855 22.48467032815171 4 21.919680695242143 27.596809170936048 28.60199797409279 21.88151215972902 5 20.819229890770004 28.86899948144423 28.091901021525455 22.21986960626031 6 20.809040832098226 28.971791614201138 27.727576541663474 22.491591012037166 7 22.208194544044797 28.72522460051141 28.831090248367225 20.235490607076564 8 20.54329825972764 28.05077705679923 29.696533474149543 21.70939120932359 9 21.386275279666386 28.014611103421714 29.065719835528576 21.53339378138332 10-11 22.071669855780442 28.404272992575475 27.811361642649878 21.712695508994205 12-13 21.69030471487555 28.582074665683066 27.936540944179516 21.791079675261866 14-15 21.708562322551376 28.76296804627264 27.90607261144783 21.622397019728155 16-17 21.999459076731316 28.67615774383328 27.700323936677684 21.62405924275772 18-19 22.027981266175843 28.69167649805689 27.6053949921368 21.674947243630466 20-21 22.000293877251146 28.767368604725913 27.597920818448145 21.634416699574796 22-23 21.811267499519932 28.584823229237394 27.9110739102911 21.692835360951573 24-25 21.756547228686514 28.583900361662153 28.034950528565407 21.62460188108593 26-27 21.603827743176367 28.66796288096665 28.04802418110125 21.680185194755733 28-29 21.969830414380116 28.588632497171005 27.934850974672422 21.50668611377646 30-31 21.966867860777622 28.42606401023654 27.79024204767185 21.81682608131399 32-33 21.64079518227729 28.77390077278142 27.913173371581763 21.67213067335953 34-35 21.92056112129764 28.529518927258486 27.94984835296234 21.60007159848153 36-37 21.883099681119123 28.52450217798925 27.895425269086616 21.696972871805016 38-39 21.70930379719707 28.54729954562856 28.143912638681872 21.599484018492497 40-41 22.048789448695842 28.511141322271815 27.775852998165046 21.664216230867297 42-43 21.770894181221127 28.705959366158062 27.92145367789896 21.60169277472185 44-45 21.66029179090648 28.479687945961373 28.20606351910276 21.653956744029387 46-47 21.934351328278552 28.600447963498738 27.84775828741497 21.61744242080774 48-49 21.817334410022497 28.473725501508994 28.089078824509656 21.619861263958853 50-51 21.89396761895116 28.53769831222103 27.84527848714814 21.723055581679677 52-53 21.778270795239294 28.59753716335819 28.02774614679937 21.596445894603146 54-55 21.700071740157828 28.491156013876562 28.095009809021583 21.71376243694403 56-57 21.66313063537281 28.63113319736177 28.130951541828253 21.574784625437168 58-59 21.8025207253968 28.551977473406986 28.01392406722718 21.63157773396904 60-61 21.86161892271959 28.366157061822978 27.95091902677739 21.821304988680048 62-63 21.72596588961818 28.497160303662046 28.081710188679583 21.69516361804019 64-65 21.83264627700012 28.498395416931434 27.99867371585488 21.670284590213566 66-67 21.897798950398077 28.457117389312103 27.951043337247267 21.694040323042557 68-69 21.716235247570765 28.481689930454234 28.156132226444612 21.645942595530382 70-71 22.01961775428564 28.418166571783615 27.883615019496162 21.678600654434582 72-73 21.849184617433036 28.527101267215077 28.00780448096372 21.615909634388167 74-75 21.763990874519294 28.418247041835215 28.097465550063205 21.720296533582285 76-77 21.99237054443684 28.548995382006947 27.856072922361303 21.60256115119491 78-79 21.94625464755164 28.423270749335405 28.020866471331612 21.60960813178134 80-81 21.969238184280815 28.406994932534204 27.907678850749633 21.71608803243535 82-83 21.947730373068964 28.46650007131497 27.945252457236723 21.640517098379338 84-85 21.969400655423563 28.219566225450837 27.96471638716962 21.846316731955984 86-87 21.843351283797414 28.464676363866342 27.99965081886095 21.692321533475294 88-89 22.02059676010924 28.352606522797196 27.987952939248594 21.63884377784497 90-91 22.112586080168477 28.304346209332987 27.74460662250889 21.838461087989646 92-93 21.927590340475916 28.411740100048604 27.929459204019235 21.73121035545624 94-95 22.113727883891787 28.334589804457632 27.789237380511384 21.7624449311392 96-97 22.15332442006633 28.426525507434285 27.722456318924944 21.69769375357444 98-99 22.013349370565358 28.42407926720268 27.883190002241744 21.679381359990224 100-101 22.434102307904823 28.257568983728515 27.500486652780985 21.80784205558567 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 184.0 1 181.0 2 190.5 3 204.0 4 206.5 5 228.5 6 279.0 7 347.5 8 442.0 9 590.5 10 777.5 11 1045.0 12 1399.0 13 1882.5 14 2460.5 15 3175.5 16 4043.0 17 5075.0 18 6370.5 19 8038.0 20 10129.5 21 12714.5 22 15916.5 23 19454.5 24 23372.5 25 27780.0 26 32631.5 27 37556.0 28 42658.5 29 47983.5 30 53064.0 31 57888.5 32 62445.5 33 66721.5 34 70583.0 35 73741.5 36 76673.0 37 79149.5 38 79997.0 39 79036.5 40 77482.5 41 75262.0 42 71918.0 43 68012.5 44 64205.5 45 60260.0 46 56283.5 47 52615.0 48 48827.5 49 45114.5 50 41568.5 51 38423.0 52 35523.5 53 32915.5 54 30817.5 55 29311.5 56 28170.5 57 27197.5 58 26313.5 59 25397.5 60 24411.5 61 23851.5 62 23360.5 63 22545.0 64 21824.5 65 21098.0 66 20179.0 67 19188.5 68 18137.0 69 16941.0 70 15515.0 71 13757.5 72 12068.0 73 10317.5 74 8548.5 75 7094.5 76 5737.5 77 4496.5 78 3497.0 79 2702.5 80 2113.5 81 1711.5 82 1376.0 83 1102.0 84 846.0 85 652.0 86 540.5 87 428.0 88 332.5 89 260.5 90 205.0 91 151.5 92 132.0 93 97.0 94 57.0 95 46.5 96 28.5 97 16.5 98 16.5 99 9.0 100 2.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 13.803352196912092 2 11.160196855140796 3 9.850988257833784 4 11.756492985000051 5 9.22158928357703 6 5.4077767658076095 7 9.85023187563653 8 10.181527278034084 9 5.490578369871784 10-11 6.396746488718115 12-13 5.428733001978607 14-15 7.415081638110273 16-17 2.6935882371000126 18-19 3.170731924356441 20-21 3.103881086040255 22-23 3.148641114889562 24-25 3.5246965461364224 26-27 4.438094788927098 28-29 1.7422596517705349 30-31 2.2907702239914647 32-33 3.0922461481236603 34-35 2.063455127181217 36-37 3.1739799184975936 38-39 1.3874051685820192 40-41 1.17940006433698 42-43 0.5619697260250203 44-45 1.3223340530829026 46-47 1.3921436805824676 48-49 1.2908107126855528 50-51 1.59627788770038 52-53 2.473681236515819 54-55 1.8537370397247481 56-57 1.8439040711604369 58-59 1.575054693106822 60-61 1.4427323016576783 62-63 0.6202111552136312 64-65 1.2371520697508966 66-67 0.5690218776876595 68-69 0.813756011569978 70-71 1.0176677533357568 72-73 0.0848927783742321 74-75 0.10673887595376458 76-77 0.4637067799875509 78-79 0.9088821961423619 80-81 0.3014850452116335 82-83 0.48871188556738226 84-85 0.06729576902045391 86-87 0.10180014513639574 88-89 0.08222319414862733 90-91 0.09043216564236203 92-93 0.17492450638275342 94-95 0.4651972978468468 96-97 0.38070495710645547 98-99 0.06751823437258764 100-101 0.23058533748661314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2247541.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.38420921895873 #Duplication Level Percentage of deduplicated Percentage of total 1 97.94966429833129 95.38750600955467 2 1.6971195037212534 3.3054528163993186 3 0.1846116640700224 0.5393478275416556 4 0.099996919464724 0.38952483705616203 5 0.03877740081565982 0.18881532569998186 6 0.017265065868030366 0.10088068720028234 7 0.011118707747223042 0.07579505930500183 8 0.0 0.0 9 0.0014464399818013905 0.012677437242937213 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 4.449307042674639E-5 2 0.0 0.0 4.449307042674639E-5 3 4.449307042674639E-5 0.0 4.449307042674639E-5 4 4.449307042674639E-5 0.0 4.449307042674639E-5 5 4.449307042674639E-5 0.0 4.449307042674639E-5 6 4.449307042674639E-5 0.0 4.449307042674639E-5 7 4.449307042674639E-5 0.0 4.449307042674639E-5 8 4.449307042674639E-5 0.0 4.449307042674639E-5 9 4.449307042674639E-5 0.0 4.449307042674639E-5 10-11 4.449307042674639E-5 0.0 4.449307042674639E-5 12-13 6.673960564011958E-5 0.0 4.449307042674639E-5 14-15 8.898614085349277E-5 0.0 8.898614085349277E-5 16-17 8.898614085349277E-5 0.0 8.898614085349277E-5 18-19 8.898614085349277E-5 0.0 8.898614085349277E-5 20-21 1.1123267606686596E-4 2.2246535213373193E-5 8.898614085349277E-5 22-23 1.3347921128023917E-4 4.449307042674639E-5 1.1123267606686596E-4 24-25 1.3347921128023917E-4 4.449307042674639E-5 1.3347921128023917E-4 26-27 1.3347921128023917E-4 8.898614085349277E-5 1.3347921128023917E-4 28-29 1.5572574649361237E-4 8.898614085349277E-5 1.3347921128023917E-4 30-31 2.0021881692035872E-4 8.898614085349277E-5 1.3347921128023917E-4 32-33 3.1145149298722474E-4 8.898614085349277E-5 2.2246535213373193E-4 34-35 4.4493070426746386E-4 8.898614085349277E-5 2.2246535213373193E-4 36-37 5.339168451209567E-4 8.898614085349277E-5 2.2246535213373193E-4 38-39 6.229029859744494E-4 8.898614085349277E-5 2.2246535213373193E-4 40-41 7.786287324680617E-4 8.898614085349277E-5 2.4471188734710513E-4 42-43 0.0010010940846017936 1.3347921128023917E-4 3.114514929872247E-4 44-45 0.0013125455775890183 1.3347921128023917E-4 3.114514929872247E-4 46-47 0.0021356673804838267 1.3347921128023917E-4 3.114514929872247E-4 48-49 0.0029587891833786346 1.3347921128023917E-4 3.559445634139711E-4 50-51 0.00418234862011416 1.3347921128023917E-4 4.226841690540907E-4 52-53 0.005650619944196791 1.3347921128023917E-4 4.4493070426746386E-4 54-55 0.007341356620413153 1.3347921128023917E-4 4.4493070426746386E-4 56-57 0.010322392339005162 1.3347921128023917E-4 4.4493070426746386E-4 58-59 0.013392414198450662 1.3347921128023917E-4 4.4493070426746386E-4 60-61 0.017330050931217717 1.3347921128023917E-4 4.4493070426746386E-4 62-63 0.022802698593707524 1.5572574649361237E-4 4.4493070426746386E-4 64-65 0.02843107200269094 1.7797228170698555E-4 4.6717723948083706E-4 66-67 0.036039387045664575 1.7797228170698555E-4 5.339168451209567E-4 68-69 0.04665098434244359 1.7797228170698555E-4 5.339168451209567E-4 70-71 0.0585083876111715 1.7797228170698555E-4 5.339168451209567E-4 72-73 0.07165608992227505 1.7797228170698555E-4 5.339168451209567E-4 74-75 0.08802953983931773 2.0021881692035872E-4 5.339168451209567E-4 76-77 0.10600474029172327 2.2246535213373193E-4 5.561633803343298E-4 78-79 0.12793982401210924 2.4471188734710513E-4 5.78409915547703E-4 80-81 0.1571717712824816 2.6695842256047834E-4 5.78409915547703E-4 82-83 0.1888730839615384 2.6695842256047834E-4 5.78409915547703E-4 84-85 0.22164223033083713 2.6695842256047834E-4 5.78409915547703E-4 86-87 0.2603956946725332 2.6695842256047834E-4 5.78409915547703E-4 88-89 0.30640152949378896 2.6695842256047834E-4 5.78409915547703E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTGTA 635 1.328962E-4 5.7122006 94-95 GATCGGA 1510 0.0 5.705108 94-95 TCGGAAG 1415 3.6379788E-12 5.607494 94-95 CGTGTAG 565 0.0011139049 5.6071506 90-91 ATCGGAA 1510 1.8189894E-12 5.5549736 94-95 GTCCCAG 1555 6.498261E-4 5.445944 1 AGATCGG 1785 0.0 5.3340077 92-93 GGTTTAA 1420 0.0073730745 5.1685333 1 GTCGTGT 770 3.764011E-4 5.0051303 94-95 >>END_MODULE