FastQCFastQC Report
Thu 11 Sep 2014
LONLib29_CAACTA_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib29_CAACTA_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247541
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA6351.328962E-45.712200694-95
GATCGGA15100.05.70510894-95
TCGGAAG14153.6379788E-125.60749494-95
CGTGTAG5650.00111390495.607150690-91
ATCGGAA15101.8189894E-125.554973694-95
GTCCCAG15556.498261E-45.4459441
AGATCGG17850.05.334007792-93
GGTTTAA14200.00737307455.16853331
GTCGTGT7703.764011E-45.005130394-95
Produced by FastQC (version 0.11.2)