##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename LONLib29_CAACTA_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2247541 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.258617306647576 33.0 33.0 33.0 27.0 33.0 2 31.654307084943056 33.0 33.0 33.0 27.0 33.0 3 31.819053356534987 33.0 33.0 33.0 27.0 33.0 4 35.66319368589939 37.0 37.0 37.0 33.0 37.0 5 35.181287015453776 37.0 37.0 37.0 33.0 37.0 6 34.99885964260496 37.0 37.0 37.0 33.0 37.0 7 34.806095639634606 37.0 33.0 37.0 27.0 37.0 8 34.85003032202749 37.0 33.0 37.0 27.0 37.0 9 34.74587293401989 37.0 33.0 37.0 27.0 37.0 10-11 34.59976503209508 37.0 33.0 37.0 27.0 37.0 12-13 34.617219441158134 37.0 33.0 37.0 27.0 37.0 14-15 35.58215200523594 37.0 33.0 40.0 27.0 40.0 16-17 35.4947300182733 37.0 33.0 40.0 27.0 40.0 18-19 35.70847339381128 37.0 33.0 40.0 27.0 40.0 20-21 35.402266299035254 37.0 33.0 40.0 27.0 40.0 22-23 35.44451647378179 37.0 33.0 40.0 27.0 40.0 24-25 35.29661394386132 37.0 33.0 40.0 27.0 40.0 26-27 34.94956421262171 37.0 33.0 40.0 27.0 40.0 28-29 34.60692129754251 37.0 33.0 40.0 27.0 40.0 30-31 34.51983545572695 37.0 33.0 40.0 27.0 40.0 32-33 34.521300835001455 37.0 33.0 40.0 27.0 40.0 34-35 34.399525080966264 37.0 33.0 40.0 24.5 40.0 36-37 34.12459750456165 37.0 33.0 40.0 22.0 40.0 38-39 34.056824324895516 37.0 33.0 38.5 24.5 40.0 40-41 33.793316339946635 37.0 33.0 37.0 22.0 40.0 42-43 33.44754289243222 37.0 33.0 37.0 22.0 40.0 44-45 33.355985941969465 37.0 33.0 37.0 22.0 40.0 46-47 33.23321977218659 37.0 33.0 37.0 22.0 40.0 48-49 33.26175829495435 37.0 33.0 37.0 22.0 40.0 50-51 33.5376529282447 37.0 33.0 37.0 22.0 40.0 52-53 33.40039380816634 37.0 33.0 37.0 22.0 40.0 54-55 33.06205960202728 37.0 33.0 37.0 22.0 40.0 56-57 33.13201383200573 37.0 33.0 37.0 22.0 40.0 58-59 32.85598371731595 37.0 33.0 37.0 22.0 40.0 60-61 32.7669346187678 37.0 33.0 37.0 22.0 40.0 62-63 32.75614126727832 37.0 33.0 37.0 22.0 40.0 64-65 32.430888913706134 37.0 33.0 37.0 22.0 40.0 66-67 32.09485744642701 33.0 27.0 37.0 22.0 40.0 68-69 31.92504875328192 33.0 27.0 37.0 22.0 40.0 70-71 31.762190990064255 33.0 27.0 37.0 22.0 40.0 72-73 31.534983566484435 33.0 27.0 37.0 22.0 38.5 74-75 31.191008973807374 33.0 27.0 37.0 15.0 37.0 76-77 30.130498620492354 33.0 27.0 37.0 15.0 37.0 78-79 31.035251637233756 33.0 27.0 37.0 22.0 37.0 80-81 30.745561482526902 33.0 27.0 37.0 15.0 37.0 82-83 30.713268189545822 33.0 27.0 37.0 15.0 37.0 84-85 30.58197937212269 33.0 27.0 37.0 15.0 37.0 86-87 30.379504534066342 33.0 27.0 37.0 15.0 37.0 88-89 30.249074877833152 33.0 27.0 37.0 15.0 37.0 90-91 30.166147358379668 33.0 27.0 37.0 15.0 37.0 92-93 29.953823979184364 33.0 27.0 37.0 15.0 37.0 94-95 29.869355442236646 33.0 27.0 37.0 15.0 37.0 96-97 29.73599814196938 33.0 27.0 33.0 15.0 37.0 98-99 29.48150000378191 33.0 27.0 33.0 15.0 37.0 100-101 28.70511394452871 33.0 27.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.1505131951633523 2306 2 0.5889842058958159 2306 3 0.5074327110308197 2306 4 0.42128929531921955 2306 5 0.637093698396825 2306 6 0.6947066339107408 2306 7 0.7291608071118034 2306 8 0.7866667639252256 2306 9 0.9542311325013273 2306 10-11 1.1411837081450642 2306 12-13 1.2948129148096257 2306 14-15 1.433242525973732 2306 16-17 1.4082414173384166 2306 18-19 1.2780922588277264 2306 20-21 1.5078636223328203 2306 22-23 1.1494948436625805 2306 24-25 1.1520574467037221 2306 26-27 1.3362424842675438 2306 28-29 1.5823054351470986 2306 30-31 1.5083897486061986 2306 32-33 1.2369025112226453 2306 34-35 1.2427454042662376 2306 36-37 1.3194187390545125 2306 38-39 1.1457638216702293 2306 40-41 1.3304877201733731 2306 42-43 1.2755117300807512 2306 44-45 0.9964690424408928 2306 46-47 0.9615864782388286 2306 48-49 1.0078398668168376 2306 50-51 1.1039660794038824 2306 52-53 1.133214369315155 2306 54-55 1.61034416736657 2306 56-57 1.173964542176364 2306 58-59 1.1829468908746676 2306 60-61 0.9003012570395441 2306 62-63 0.6520119931602011 2306 64-65 0.8038927308583439 2306 66-67 1.1064095559138174 2306 68-69 0.817018316479416 2306 70-71 0.6856197440157281 2306 72-73 0.5458558664475035 2306 74-75 0.8272964532350144 2306 76-77 0.8043568994208492 2306 78-79 0.8283407534991056 2306 80-81 1.2665296180058903 2306 82-83 1.1596956722242453 2306 84-85 1.063935684397432 2306 86-87 0.8705254430021512 2306 88-89 0.6476702886625425 2306 90-91 0.4027888913195561 2306 92-93 0.30895333482938625 2306 94-95 0.3045815078217622 2306 96-97 0.3997758042614237 2306 98-99 0.5292091830591978 2306 100-101 0.3718918515280123 2307 1 0.7107111778652069 2307 2 0.5221704189775416 2307 3 0.4179089629350905 2307 4 0.3087315529240158 2307 5 0.49948097787002155 2307 6 0.6267211063663893 2307 7 0.7795886536890322 2307 8 0.7817494461209122 2307 9 0.9547471283733628 2307 10-11 1.0860942381465293 2307 12-13 1.3576599834616516 2307 14-15 1.1829630282097199 2307 16-17 1.2522279154464329 2307 18-19 1.0827924283692312 2307 20-21 1.4423359858396196 2307 22-23 1.1889431887901054 2307 24-25 1.2104325511314613 2307 26-27 1.3715544160578048 2307 28-29 1.4517423546517492 2307 30-31 1.536000902716971 2307 32-33 1.1735179468705894 2307 34-35 1.1568144309997308 2307 36-37 1.2113913175596025 2307 38-39 0.9120330486592678 2307 40-41 1.034318375242421 2307 42-43 1.1622659931895 2307 44-45 1.0667634979423894 2307 46-47 1.0709069608178297 2307 48-49 1.0714425008529034 2307 50-51 0.9371719494712067 2307 52-53 1.0451073063145202 2307 54-55 1.5060415910729006 2307 56-57 1.116671339807695 2307 58-59 1.1029475665835449 2307 60-61 0.8927440674970484 2307 62-63 0.8036026732918984 2307 64-65 1.0065140206023173 2307 66-67 1.1513242323106887 2307 68-69 0.9445000109115718 2307 70-71 0.8639139783761358 2307 72-73 0.7635462320674407 2307 74-75 1.002318106633215 2307 76-77 1.1559934970423562 2307 78-79 1.0313636293161004 2307 80-81 1.5105841477125104 2307 82-83 1.369921758272703 2307 84-85 1.1150814716597068 2307 86-87 1.1124768288196378 2307 88-89 1.0445233281239439 2307 90-91 0.7709051071041166 2307 92-93 0.8257861644524631 2307 94-95 0.7434544254098547 2307 96-97 0.7607210595565235 2307 98-99 0.8555911612890839 2307 100-101 0.7958584776521462 1101 1 1.171553706389993 1101 2 0.6153046730899376 1101 3 0.3513940678086449 1101 4 0.300991962085142 1101 5 0.6196905399528276 1101 6 0.6617616008036364 1101 7 0.7347474587379352 1101 8 0.7057310763548301 1101 9 0.9020776197568523 1101 10-11 1.0415268548778442 1101 12-13 1.2208420060495868 1101 14-15 1.3161094268647204 1101 16-17 1.3242589783036038 1101 18-19 1.0734192406012468 1101 20-21 1.290841935731578 1101 22-23 1.004522683649732 1101 24-25 1.0864096696290346 1101 26-27 1.2203833848800514 1101 28-29 1.4257005530726374 1101 30-31 1.3707260698850448 1101 32-33 0.973610815088378 1101 34-35 1.132686359643678 1101 36-37 1.2889790549195936 1101 38-39 0.8918412707895129 1101 40-41 1.1347735841357718 1101 42-43 1.26380594216198 1101 44-45 1.0690262604170186 1101 46-47 0.9530344126119061 1101 48-49 1.0542749422883517 1101 50-51 1.0262006142569788 1101 52-53 1.27352061089708 1101 54-55 1.5870727564855471 1101 56-57 1.2192431652089297 1101 58-59 1.1885917022337864 1101 60-61 1.0364754649987518 1101 62-63 0.7985420800947836 1101 64-65 0.8112934276377999 1101 66-67 0.9453411728198695 1101 68-69 0.8344436617845155 1101 70-71 0.9434753360697421 1101 72-73 0.8536363548996562 1101 74-75 1.0342549981836129 1101 76-77 1.120975491683037 1101 78-79 0.9560191169066208 1101 80-81 1.634751919670002 1101 82-83 1.3929889442718704 1101 84-85 1.2038349370922532 1101 86-87 0.9082192829300268 1101 88-89 0.8812112565507881 1101 90-91 0.904707279341995 1101 92-93 0.8245634502458721 1101 94-95 0.7642177914992665 1101 96-97 0.8721882734324531 1101 98-99 0.945943465800628 1101 100-101 0.9540086340316307 2201 1 1.4115163864273974 2201 2 0.7941109162531319 2201 3 0.34695467651276246 2201 4 0.25973893566559525 2201 5 0.42245938358517776 2201 6 0.39951012780517914 2201 7 0.6802347954247594 2201 8 0.6484921927534515 2201 9 0.5981027650762556 2201 10-11 0.8240665664885114 2201 12-13 1.0574761437904598 2201 14-15 1.10827650231181 2201 16-17 1.1203188514833826 2201 18-19 0.9610062168912208 2201 20-21 1.2473730036715551 2201 22-23 1.044481017227696 2201 24-25 0.9534065211624778 2201 26-27 1.1158186998805064 2201 28-29 1.417571490918533 2201 30-31 1.4363017431020992 2201 32-33 1.0726231829562352 2201 34-35 0.9350108741919101 2201 36-37 1.038878389692222 2201 38-39 0.6706844982915214 2201 40-41 1.1156228516390598 2201 42-43 1.0613041560388012 2201 44-45 0.9068822045552025 2201 46-47 0.8916765138376164 2201 48-49 0.9338569846458284 2201 50-51 0.9990693966620938 2201 52-53 1.0767985861690477 2201 54-55 1.4962943660653636 2201 56-57 0.9648883370855472 2201 58-59 1.0910716991001337 2201 60-61 0.8909841542809716 2201 62-63 0.8818642113636415 2201 64-65 0.8579294914911415 2201 66-67 0.9726508055642036 2201 68-69 0.8698914045042692 2201 70-71 0.8909375076327315 2201 72-73 0.6754695714364942 2201 74-75 0.7467526332627656 2201 76-77 0.8535424431508751 2201 78-79 0.7998686840176639 2201 80-81 1.3418822031266302 2201 82-83 1.176777649558872 2201 84-85 0.9867046422436907 2201 86-87 0.9841388723788889 2201 88-89 0.9761890628804437 2201 90-91 0.8443930785213745 2201 92-93 0.9002684770751799 2201 94-95 0.8883601653864375 2201 96-97 0.7725963630347152 2201 98-99 0.8555409214393563 2201 100-101 0.8901047046307511 2304 1 -1.9420577054366355 2304 2 -0.3513797814446491 2304 3 -0.22840714278662944 2304 4 0.04422103899460694 2304 5 0.32429537980545575 2304 6 0.5623652904332843 2304 7 0.5730332272680911 2304 8 0.7139504797249003 2304 9 0.9105000754097219 2304 10-11 0.9705760789209208 2304 12-13 1.1649341494233454 2304 14-15 1.2212352993186144 2304 16-17 1.2526937224455281 2304 18-19 0.9342378795365747 2304 20-21 1.183003374605967 2304 22-23 0.9017135479161027 2304 24-25 1.2009095322277332 2304 26-27 1.155677908279415 2304 28-29 1.5171144468169686 2304 30-31 1.4117342028442508 2304 32-33 1.0093595224182934 2304 34-35 1.043837456491957 2304 36-37 1.2611931354587114 2304 38-39 1.0031546486634824 2304 40-41 1.0384602362039672 2304 42-43 1.0040701618656556 2304 44-45 0.9978668873306873 2304 46-47 1.0589491614824738 2304 48-49 0.9437458275186756 2304 50-51 0.8973988179041186 2304 52-53 1.246941083712798 2304 54-55 1.6258989368558687 2304 56-57 1.1285560067853453 2304 58-59 0.8216212223317498 2304 60-61 0.5226949963438869 2304 62-63 0.4911074499549031 2304 64-65 0.4443360335737552 2304 66-67 0.613658762115751 2304 68-69 0.5490779647340922 2304 70-71 0.5812478625081319 2304 72-73 0.350489404931249 2304 74-75 0.5505215542638595 2304 76-77 0.671729801525963 2304 78-79 0.597346804920722 2304 80-81 1.282039929648949 2304 82-83 1.1173334657748981 2304 84-85 0.6296305283181773 2304 86-87 0.559716774108729 2304 88-89 0.5509313038993433 2304 90-91 0.40900106993442975 2304 92-93 0.4634456391117716 2304 94-95 0.19089828479543414 2304 96-97 0.17312068374844358 2304 98-99 0.5539854118998413 2304 100-101 0.52622211018247 1102 1 1.446141420137554 1102 2 0.1532063421187253 1102 3 0.19329743411303468 1102 4 0.17232386704264258 1102 5 0.3303414624147152 1102 6 0.11758159091107956 1102 7 -4.568773919410383 1102 8 -1.0251177217988925 1102 9 0.11761262544938944 1102 10-11 0.7002671704773604 1102 12-13 0.9321503761099379 1102 14-15 0.7912086422448041 1102 16-17 0.6682220674932111 1102 18-19 0.9703905904410774 1102 20-21 1.1734387427318538 1102 22-23 0.7768755196171782 1102 24-25 1.102729961323604 1102 26-27 1.0581834887395019 1102 28-29 0.9099002493885919 1102 30-31 1.2181938851731005 1102 32-33 1.0009573787837098 1102 34-35 1.1131803457866951 1102 36-37 1.3643946592471465 1102 38-39 0.9520873510819925 1102 40-41 1.2377770218589603 1102 42-43 1.2941916406414649 1102 44-45 1.2879372656950991 1102 46-47 1.1042068772756366 1102 48-49 1.0712419995997706 1102 50-51 0.819431765678388 1102 52-53 0.9554247666319782 1102 54-55 1.4290156929943691 1102 56-57 1.1917507048870633 1102 58-59 1.0883945172936578 1102 60-61 0.9080949446900348 1102 62-63 0.703928487828243 1102 64-65 0.5838615561077418 1102 66-67 0.5790226450091005 1102 68-69 0.7109244052307027 1102 70-71 0.6023726249926789 1102 72-73 0.6113783122679379 1102 74-75 0.6068335619381457 1102 76-77 0.5221129623723471 1102 78-79 0.7475916994915366 1102 80-81 1.3456969296673975 1102 82-83 1.233990262867522 1102 84-85 0.7872911625536076 1102 86-87 0.6726982156199739 1102 88-89 0.4903629271214385 1102 90-91 0.18787252565048362 1102 92-93 0.337502956432413 1102 94-95 0.2620049684340806 1102 96-97 0.16908780976537585 1102 98-99 0.35067052636845375 1102 100-101 0.39250008166217043 2202 1 1.4688345346335332 2202 2 0.8772267974983947 2202 3 0.31607136695707183 2202 4 -0.4366474678552379 2202 5 -0.07376376420385355 2202 6 0.33886096562354595 2202 7 0.5498654889182575 2202 8 -3.9182917436660176 2202 9 -0.34631990302221993 2202 10-11 0.5307759806073875 2202 12-13 0.035425263128679774 2202 14-15 0.06721100733579277 2202 16-17 0.17971209142647382 2202 18-19 0.6095086971724513 2202 20-21 1.1255130305056298 2202 22-23 -2.740850438751245 2202 24-25 -2.9115647881411775 2202 26-27 -1.4777555304453003 2202 28-29 0.4239328125725592 2202 30-31 1.1981638897073026 2202 32-33 0.9165790040861879 2202 34-35 -2.342739207902028 2202 36-37 -0.573065060296905 2202 38-39 0.10585237995636021 2202 40-41 1.0680283958207042 2202 42-43 1.0412379841343906 2202 44-45 1.0489860772534882 2202 46-47 0.4158382087521062 2202 48-49 0.8078544385832487 2202 50-51 -4.179583034409966 2202 52-53 -0.48320444390263617 2202 54-55 1.429638688521628 2202 56-57 1.1031189864258977 2202 58-59 1.2305329315865166 2202 60-61 1.1423377750927557 2202 62-63 1.058249811349107 2202 64-65 0.9367760158820957 2202 66-67 1.1646139592788707 2202 68-69 1.0908429225312481 2202 70-71 1.2133215014914107 2202 72-73 0.9521325504146603 2202 74-75 0.6838793241235948 2202 76-77 1.0479680905838649 2202 78-79 0.9913063539208515 2202 80-81 1.7266906000594133 2202 82-83 1.5663061088110766 2202 84-85 1.3993393745348541 2202 86-87 1.3058012183222232 2202 88-89 1.2198535522927152 2202 90-91 1.0325279348826442 2202 92-93 1.0843523529522088 2202 94-95 0.8800897097771667 2202 96-97 0.8023013605409233 2202 98-99 1.0924494601176704 2202 100-101 1.0798130625903006 2305 1 1.3237968150281247 2305 2 0.7138542997498405 2305 3 0.21942373665828896 2305 4 -0.1430629823942411 2305 5 0.2503440481907404 2305 6 0.1883108901184798 2305 7 0.45160622119907146 2305 8 -0.13203665929721353 2305 9 0.48205032164908346 2305 10-11 0.2227618767316244 2305 12-13 0.8162816263168793 2305 14-15 0.6230948239523286 2305 16-17 0.6136931911548018 2305 18-19 0.5479639342436471 2305 20-21 0.6437108956584296 2305 22-23 0.41772374875477425 2305 24-25 0.773634841029839 2305 26-27 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-0.9154526549919524 1208 42-43 -0.25455230193284706 1208 44-45 -0.058796432163532586 1208 46-47 -0.4535035237885694 1208 48-49 -1.83023130453698 1208 50-51 -0.38340099322526555 1208 52-53 -1.982691449560889 1208 54-55 -1.2453673349842447 1208 56-57 -6.653268341991577 1208 58-59 -2.0707473797927314 1208 60-61 -1.8970351873855975 1208 62-63 -0.0934001118590615 1208 64-65 -0.26624305055909403 1208 66-67 0.6041344644395075 1208 68-69 0.4633084179458997 1208 70-71 -4.1244420976830405 1208 72-73 -0.7602412338894311 1208 74-75 -5.773454257561369 1208 76-77 -1.410310094284558 1208 78-79 -3.7798746769369806 1208 80-81 0.6915884186063863 1208 82-83 0.10625576167115725 1208 84-85 -3.0286957767296094 1208 86-87 -1.1465038022262206 1208 88-89 -5.291557827497755 1208 90-91 -0.4834108279822438 1208 92-93 -2.2820898463524877 1208 94-95 -4.75098128844396 1208 96-97 -3.5238371170272345 1208 98-99 -6.351321060180389 1208 100-101 -4.034067765824595 1209 1 1.702388818293965 1209 2 1.108187014137755 1209 3 0.588214670707341 1209 4 0.17563043855617622 1209 5 0.3517033416891735 1209 6 1.0065973506494146 1209 7 0.8124214418741857 1209 8 0.127706145779662 1209 9 0.031637801383574526 1209 10-11 -3.025273517400528 1209 12-13 -0.32937009172632514 1209 14-15 -1.3826777686978104 1209 16-17 -0.6011643930083252 1209 18-19 -16.79454746090197 1209 20-21 -13.957997474930949 1209 22-23 -3.513694408026794 1209 24-25 -2.2347127981173713 1209 26-27 -1.121986993964427 1209 28-29 -3.033352798485332 1209 30-31 -0.37091504534527786 1209 32-33 -0.37230458969682445 1209 34-35 -2.809779313247894 1209 36-37 -3.7409418458574173 1209 38-39 -1.8593250782965391 1209 40-41 -10.29483076880414 1209 42-43 -5.350270645016181 1209 44-45 -2.741544347943517 1209 46-47 -2.4756642695178996 1209 48-49 -2.850169438600247 1209 50-51 -2.0762744490108744 1209 52-53 -0.9874635275497994 1209 54-55 -2.374096607530955 1209 56-57 -0.44571980755222285 1209 58-59 -1.7194120046609456 1209 60-61 -1.5646476462505916 1209 62-63 -1.2853945372485747 1209 64-65 0.057989814607104506 1209 66-67 -0.016869911240281255 1209 68-69 -1.4136092452797513 1209 70-71 -0.7834158575130203 1209 72-73 -0.37085580409641494 1209 74-75 0.6583986328074083 1209 76-77 -16.788318200280045 1209 78-79 -17.04985105994944 1209 80-81 -22.358541349646707 1209 82-83 -9.761943922879329 1209 84-85 -3.509083106452241 1209 86-87 -2.109046258559829 1209 88-89 -1.9980938079365487 1209 90-91 -6.16600992057808 1209 92-93 -3.1134634426160552 1209 94-95 -0.7239163319921254 1209 96-97 -0.034726245145911605 1209 98-99 0.7064223352692345 1209 100-101 0.4769326319644769 1212 1 -28.647893667581744 1212 2 -16.27475382231877 1212 3 -5.619207342680436 1212 4 -1.456270887169616 1212 5 -0.2904952001989187 1212 6 -2.398758227281263 1212 7 -0.1249189023297248 1212 8 -1.1049764445132695 1212 9 0.07670735071138779 1212 10-11 0.7274604424361399 1212 12-13 0.3006491601988728 1212 14-15 -0.17100098661281748 1212 16-17 -0.17057620707108612 1212 18-19 -1.0177544214285277 1212 20-21 -0.934322967649706 1212 22-23 -0.5559274448898037 1212 24-25 -0.06837709675062342 1212 26-27 -0.7929678632377986 1212 28-29 -0.021449701395965803 1212 30-31 -0.38007952111489374 1212 32-33 0.34790037571497834 1212 34-35 -1.1822924120153928 1212 36-37 -1.4707110092144333 1212 38-39 -0.5511882264757588 1212 40-41 0.08706006858468385 1212 42-43 0.2613963479543031 1212 44-45 1.120638904280014 1212 46-47 -2.025333537292134 1212 48-49 -1.2521501027683541 1212 50-51 -0.10451487898608036 1212 52-53 -1.0381489986392864 1212 54-55 -0.34258265105056296 1212 56-57 -1.2611926683976336 1212 58-59 -0.447304921071229 1212 60-61 0.1941742105113562 1212 62-63 -4.571166758538503 1212 64-65 -4.237061088788323 1212 66-67 -1.9861553991111016 1212 68-69 0.07830657765847349 1212 70-71 -1.1649442645910177 1212 72-73 -1.396337801448695 1212 74-75 -0.2946206097948192 1212 76-77 0.9675179765579749 1212 78-79 -1.343430100056839 1212 80-81 0.5031594390762351 1212 82-83 0.3551199021472442 1212 84-85 -3.73316176431803 1212 86-87 -1.9064535416610227 1212 88-89 -0.9472708230956925 1212 90-91 -0.04844731686008075 1212 92-93 -2.610202735189379 1212 94-95 -2.1481299397935842 1212 96-97 -0.047422959732625 1212 98-99 -0.9262737733246738 1212 100-101 -0.33815332578383916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 7.0 10 11.0 11 10.0 12 52.0 13 145.0 14 513.0 15 1277.0 16 2478.0 17 4080.0 18 6364.0 19 9179.0 20 12896.0 21 17299.0 22 23201.0 23 29627.0 24 37824.0 25 47596.0 26 58998.0 27 72435.0 28 86721.0 29 104567.0 30 124492.0 31 146304.0 32 170927.0 33 197429.0 34 223994.0 35 250110.0 36 269521.0 37 255750.0 38 93513.0 39 220.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.55269338789975 27.10050017724227 17.74896489168951 19.597841543168464 2 25.118354990220137 30.65532179209033 25.362228562173744 18.864094655515785 3 18.23731724463191 27.04690461571318 32.948646075264506 21.767132064390402 4 21.748622938857594 26.991822170017 30.086361088123013 21.173193803002395 5 20.162479794584392 28.849707302336196 28.923209854681183 22.064603048398226 6 20.212890443377894 29.04703406967882 28.615807231102792 22.124268255840494 7 21.564100499167758 28.74434771156566 29.592697085392434 20.098854703874146 8 20.02112530983862 27.926965514755903 30.36594215633886 21.685967019066617 9 20.92500203555797 27.86916901627156 29.538415539471806 21.667413408698664 10-11 21.41551589047764 28.453874701284647 28.408691988266288 21.721917419971426 12-13 21.32580238138383 28.452680020799942 28.407166217435176 21.814351380381055 14-15 21.222237992543853 28.616808325187392 28.376901689446377 21.784051992822377 16-17 21.513656878071288 28.49936852914788 28.30226330632665 21.684711286454178 18-19 21.753510051452764 28.528966764066322 28.028649263021162 21.688873921459752 20-21 21.53753813612299 28.547999791772433 28.117262376971098 21.797199695133482 22-23 21.386021742433563 28.401223470722776 28.428942659765195 21.783812127078466 24-25 21.453357246875584 28.30333239749575 28.50368469362739 21.739625662001274 26-27 21.47395753848317 28.438680317733915 28.392607743307018 21.694754400475897 28-29 21.736533393606614 28.33561212009036 28.34493341834476 21.58292106795827 30-31 21.811259505388332 28.20691591388099 28.139931596353524 21.841892984377147 32-33 21.50034192924623 28.374410077502482 28.27959534442308 21.84565264882821 34-35 21.75526497625627 28.269918101605267 28.286113579240602 21.68870334289786 36-37 21.774330256934135 28.341129260823273 28.22891773720702 21.655622745035576 38-39 21.545524642264592 28.324021675244186 28.48243925249862 21.6480144299926 40-41 21.835043789776147 28.230684812165695 28.159094139954206 21.77517725810395 42-43 21.54814973341977 28.387958217447423 28.380216423193172 21.68367562593964 44-45 21.500230696570164 28.171899867455142 28.56873356259129 21.7591358733834 46-47 21.722542336486757 28.257264319195023 28.37537591328989 21.644817431028326 48-49 21.73758865248227 28.151712539042688 28.416869110227182 21.693829698247864 50-51 21.72401825996423 28.240120309315962 28.27233330663748 21.763528124082328 52-53 21.635874454625462 28.348708675893352 28.374314472102363 21.64110239737882 54-55 21.534218159999842 28.15871618189855 28.53440303343718 21.77266262466443 56-57 21.582494698558666 28.287383059058456 28.408651026784405 21.721471215598473 58-59 21.615571308037175 28.15616913186101 28.55592676543477 21.67233279466705 60-61 21.800982872041985 28.07874989710767 28.324178696726797 21.796088534123548 62-63 21.556444886811228 28.20167594620972 28.45313239361043 21.78874677336862 64-65 21.698711153341225 28.118339051232837 28.439655266389007 21.743294529036923 66-67 21.7127475723317 28.134344031210844 28.42529936496184 21.727609031495614 68-69 21.595490109530232 28.188951222203634 28.521644412961372 21.693914255304758 70-71 21.893784554570164 28.1557050539469 28.19345909559826 21.75705129588468 72-73 21.648072574268244 28.233962191925578 28.455204035009668 21.662761198796513 74-75 21.54124087867418 28.069334762506116 28.595125529652293 21.79429882916741 76-77 21.657040904161367 28.323183184213676 28.435488079526127 21.584287832098834 78-79 21.77235820106597 28.199210600432206 28.3980510168714 21.63038018163042 80-81 21.65893187742891 28.103122009803833 28.41837889271198 21.819567220055276 82-83 21.67640712312176 28.223333178340344 28.386253797977094 21.7140059005608 84-85 21.58800017264402 27.995696358323556 28.513695164944007 21.902608304088414 86-87 21.51544957537946 28.190683333040393 28.545633219785266 21.748233871794877 88-89 21.673218842678803 28.15215287372433 28.47246417439245 21.702164109204418 90-91 21.737994616803263 28.03125244771677 28.312695372302173 21.918057563177797 92-93 21.56722006823442 28.112856006867048 28.486111710608462 21.83381221429007 94-95 21.721368194571454 28.103658116006848 28.34834093169485 21.826632757726856 96-97 21.704641429068534 28.15191862881472 28.279183475861064 21.864256466255682 98-99 21.53539486114702 28.15845085934569 28.470212034088142 21.835942245419144 100-101 21.793896798435753 27.959531223995576 28.249223260446044 21.997348717122627 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 2.5 2 2.5 3 5.5 4 7.5 5 13.0 6 21.5 7 30.0 8 49.0 9 80.5 10 126.5 11 186.5 12 281.5 13 431.5 14 630.0 15 925.0 16 1429.5 17 2120.0 18 3089.5 19 4484.5 20 6405.0 21 8750.0 22 11587.0 23 15045.5 24 19201.5 25 23842.0 26 28777.0 27 34177.0 28 39663.0 29 45054.5 30 50562.0 31 55984.0 32 60838.0 33 65384.5 34 70101.5 35 74326.5 36 77722.0 37 80513.0 38 82374.5 39 82458.5 40 81069.5 41 78698.0 42 75317.5 43 71116.5 44 66835.5 45 62840.0 46 58888.5 47 55287.0 48 51665.5 49 47717.5 50 43813.0 51 40083.0 52 36604.0 53 33995.0 54 32028.5 55 30285.0 56 29117.0 57 28306.5 58 27532.5 59 26863.0 60 26065.0 61 25308.0 62 24891.0 63 24571.0 64 24037.0 65 23535.5 66 22898.5 67 22050.5 68 21077.0 69 19675.0 70 17847.0 71 15822.5 72 13645.0 73 11393.5 74 9082.0 75 6752.5 76 4823.5 77 3356.0 78 2197.5 79 1326.5 80 805.5 81 518.5 82 347.0 83 242.0 84 168.5 85 112.0 86 67.0 87 45.5 88 43.0 89 35.0 90 21.5 91 13.5 92 8.5 93 4.0 94 2.0 95 1.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7177622121242727 2 0.002892049577738515 3 4.449307042674639E-5 4 4.449307042674639E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.1897851919052867 14-15 0.0 16-17 4.6717723948083706E-4 18-19 0.11864077229291924 20-21 0.0 22-23 2.2246535213373193E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.001846462422709975 42-43 0.0 44-45 0.0 46-47 0.022847191664134268 48-49 4.449307042674639E-5 50-51 4.449307042674639E-5 52-53 1.3347921128023917E-4 54-55 0.0025583515495379173 56-57 0.0017352297466431091 58-59 0.09701714006552051 60-61 0.002046681239630334 62-63 0.0015350109297227502 64-65 0.002958789183378635 66-67 0.005205689239929328 68-69 0.005561633803343298 70-71 0.004427060507461266 72-73 0.0402439822009921 74-75 0.04538293183528131 76-77 0.16237746052241095 78-79 4.4493070426746386E-4 80-81 0.12035375550434896 82-83 0.005672866479410164 84-85 0.006318016000597986 86-87 0.00807549228245447 88-89 0.008720641803642293 90-91 0.02442669566428377 92-93 0.01281400428290296 94-95 0.01539460236765425 96-97 0.011523705240527314 98-99 0.013703865691437887 100-101 0.01454923402954607 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2247541.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.91427372464972 #Duplication Level Percentage of deduplicated Percentage of total 1 97.1067799039811 93.13926268229757 2 2.32333872256234 4.456826923818445 3 0.22228752279065656 0.6396163891955203 4 0.1244408433184198 0.4774261243427666 5 0.10235443331433379 0.49086255669212087 6 0.0723014487489767 0.4160844567598851 7 0.024456900492565176 0.16420360938102918 8 0.009894382633183733 0.07592100193724839 9 0.002936877264647853 0.025351960487142125 >10 0.011208964893776808 0.11444429508827052 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 4.449307042674639E-5 2 0.0 0.0 4.449307042674639E-5 3 4.449307042674639E-5 0.0 4.449307042674639E-5 4 4.449307042674639E-5 4.449307042674639E-5 4.449307042674639E-5 5 8.898614085349277E-5 8.898614085349277E-5 4.449307042674639E-5 6 8.898614085349277E-5 8.898614085349277E-5 4.449307042674639E-5 7 8.898614085349277E-5 1.3347921128023917E-4 4.449307042674639E-5 8 1.7797228170698555E-4 1.3347921128023917E-4 8.898614085349277E-5 9 1.7797228170698555E-4 1.3347921128023917E-4 8.898614085349277E-5 10-11 2.2246535213373193E-4 1.3347921128023917E-4 8.898614085349277E-5 12-13 2.2246535213373193E-4 1.3347921128023917E-4 1.1123267606686596E-4 14-15 2.2246535213373193E-4 1.3347921128023917E-4 1.3347921128023917E-4 16-17 2.2246535213373193E-4 1.3347921128023917E-4 1.3347921128023917E-4 18-19 2.2246535213373193E-4 1.7797228170698555E-4 1.3347921128023917E-4 20-21 2.6695842256047834E-4 1.7797228170698555E-4 1.3347921128023917E-4 22-23 3.114514929872247E-4 2.0021881692035872E-4 1.7797228170698555E-4 24-25 3.114514929872247E-4 2.2246535213373193E-4 2.2246535213373193E-4 26-27 3.114514929872247E-4 2.2246535213373193E-4 2.2246535213373193E-4 28-29 3.336980282005979E-4 2.2246535213373193E-4 2.2246535213373193E-4 30-31 4.004376338407175E-4 2.2246535213373193E-4 2.2246535213373193E-4 32-33 5.339168451209567E-4 2.2246535213373193E-4 2.2246535213373193E-4 34-35 6.89642591614569E-4 2.2246535213373193E-4 2.2246535213373193E-4 36-37 8.231218028948082E-4 2.4471188734710513E-4 2.892049577738515E-4 38-39 9.788475493884205E-4 2.6695842256047834E-4 3.336980282005979E-4 40-41 0.0012235594367355257 2.6695842256047834E-4 3.559445634139711E-4 42-43 0.0015350109297227505 2.6695842256047834E-4 3.781910986273443E-4 44-45 0.0020021881692035876 2.6695842256047834E-4 4.004376338407175E-4 46-47 0.0031367614650856203 2.6695842256047834E-4 4.004376338407175E-4 48-49 0.0040711159440472945 3.114514929872247E-4 4.004376338407175E-4 50-51 0.005606126873770045 3.114514929872247E-4 4.004376338407175E-4 52-53 0.007141137803492795 3.114514929872247E-4 4.004376338407175E-4 54-55 0.00892086062056265 3.114514929872247E-4 4.004376338407175E-4 56-57 0.012102115156075018 3.114514929872247E-4 4.004376338407175E-4 58-59 0.015350109297227504 3.114514929872247E-4 4.004376338407175E-4 60-61 0.019287746029994557 3.559445634139711E-4 4.004376338407175E-4 62-63 0.0252275709319652 3.559445634139711E-4 4.004376338407175E-4 64-65 0.03094493048180211 3.559445634139711E-4 4.004376338407175E-4 66-67 0.03861998513041587 3.559445634139711E-4 4.004376338407175E-4 68-69 0.04909810321591464 4.0043763384071745E-4 4.004376338407175E-4 70-71 0.06106673916070942 4.6717723948083706E-4 4.004376338407175E-4 72-73 0.0755937266550421 4.894237746942103E-4 4.004376338407175E-4 74-75 0.0925233399524191 4.894237746942103E-4 4.226841690540907E-4 76-77 0.11141064834857295 4.894237746942103E-4 4.894237746942103E-4 78-79 0.13539241330858925 4.894237746942103E-4 4.894237746942103E-4 80-81 0.16627060418475126 4.894237746942103E-4 4.894237746942103E-4 82-83 0.20037454266685234 4.894237746942103E-4 4.894237746942103E-4 84-85 0.2361024782195297 5.339168451209567E-4 4.894237746942103E-4 86-87 0.27888256543484635 5.339168451209567E-4 4.894237746942103E-4 88-89 0.3290707488762163 5.339168451209567E-4 4.894237746942103E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 1460 0.0 6.3388143 94-95 GGATCCT 840 0.005911863 6.2584987 1 GATAAGG 970 0.0039972835 5.9124336 1 CACGTCT 935 1.183671E-7 5.839061 90-91 AGATCGG 1645 0.0 5.7705145 92-93 GATCGGA 1610 0.0 5.7485614 92-93 ATCGGAA 1635 0.0 5.6603484 94-95 ACGTCTG 890 1.7311977E-6 5.6008773 90-91 CACACGT 1050 2.118868E-7 5.425612 88-89 GGTTGTG 1190 0.006554119 5.2209935 1 ACACGTC 955 6.330147E-6 5.2180977 94-95 GGTTGCA 1570 2.2893115E-4 5.1749487 1 CGTCTGA 1025 4.2165175E-6 5.0935345 92-93 >>END_MODULE