FastQCFastQC Report
Thu 11 Sep 2014
LONLib29_CAACTA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLONLib29_CAACTA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247541
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG14600.06.338814394-95
GGATCCT8400.0059118636.25849871
GATAAGG9700.00399728355.91243361
CACGTCT9351.183671E-75.83906190-91
AGATCGG16450.05.770514592-93
GATCGGA16100.05.748561492-93
ATCGGAA16350.05.660348494-95
ACGTCTG8901.7311977E-65.600877390-91
CACACGT10502.118868E-75.42561288-89
GGTTGTG11900.0065541195.22099351
ACACGTC9556.330147E-65.218097794-95
GGTTGCA15702.2893115E-45.17494871
CGTCTGA10254.2165175E-65.093534592-93
Produced by FastQC (version 0.11.2)