##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane8_Undetermined_L008_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3175716 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.9069901086873 33.0 33.0 33.0 27.0 33.0 2 31.036760528964177 33.0 33.0 33.0 27.0 33.0 3 30.58838951593908 33.0 33.0 33.0 27.0 33.0 4 34.5240421372692 37.0 37.0 37.0 33.0 37.0 5 34.679190141687734 37.0 37.0 37.0 33.0 37.0 6 34.70560591690189 37.0 37.0 37.0 33.0 37.0 7 34.695092067426685 37.0 37.0 37.0 33.0 37.0 8 34.70847834000269 37.0 37.0 37.0 33.0 37.0 9 34.660328253534004 37.0 37.0 37.0 33.0 37.0 10-11 34.66799565830193 37.0 37.0 37.0 33.0 37.0 12-13 34.648365439478845 37.0 37.0 37.0 33.0 37.0 14-15 36.790062618949555 40.0 37.0 40.0 33.0 40.0 16-17 36.80762432786811 40.0 37.0 40.0 33.0 40.0 18-19 36.78605926978357 40.0 37.0 40.0 33.0 40.0 20-21 36.726854353474934 40.0 37.0 40.0 33.0 40.0 22-23 36.62526450727962 40.0 37.0 40.0 33.0 40.0 24-25 36.576794335513625 40.0 37.0 40.0 33.0 40.0 26-27 36.480325066851066 40.0 37.0 40.0 33.0 40.0 28-29 36.41158025465754 40.0 37.0 40.0 33.0 40.0 30-31 36.287442107543626 40.0 37.0 40.0 33.0 40.0 32-33 36.188355476371314 40.0 37.0 40.0 27.0 40.0 34-35 36.169860749512864 40.0 37.0 40.0 30.0 40.0 36-37 36.306579681558425 40.0 37.0 40.0 33.0 40.0 38-39 36.26537826430324 40.0 37.0 40.0 27.0 40.0 40-41 36.205028409341395 40.0 37.0 40.0 27.0 40.0 42-43 36.10125448875151 40.0 37.0 40.0 27.0 40.0 44-45 36.00232120882346 40.0 37.0 40.0 27.0 40.0 46-47 35.86348448664805 40.0 37.0 40.0 27.0 40.0 48-49 35.67967696103808 40.0 37.0 40.0 27.0 40.0 50-51 34.74955143973831 38.5 35.0 38.5 27.0 40.0 52-53 34.85359758240347 37.0 37.0 38.5 27.0 40.0 54-55 35.580513654243646 37.0 37.0 40.0 27.0 40.0 56-57 35.52095165310752 37.0 37.0 40.0 27.0 40.0 58-59 35.32185859818699 37.0 37.0 40.0 27.0 40.0 60-61 35.05850098056627 37.0 37.0 40.0 27.0 40.0 62-63 34.797073163973096 37.0 37.0 40.0 27.0 40.0 64-65 34.5573532079065 37.0 37.0 38.5 27.0 40.0 66-67 34.302431640612696 37.0 37.0 37.0 27.0 40.0 68-69 34.05545253416867 37.0 37.0 37.0 27.0 40.0 70-71 33.83429784023508 37.0 33.0 37.0 27.0 38.5 72-73 33.63170856587932 37.0 33.0 37.0 27.0 37.0 74-75 33.44423887400511 37.0 33.0 37.0 27.0 37.0 76-77 33.271660312194165 37.0 33.0 37.0 27.0 37.0 78-79 33.12373745007425 37.0 33.0 37.0 22.0 37.0 80-81 33.003034118919956 37.0 33.0 37.0 22.0 37.0 82-83 32.89948613163142 37.0 33.0 37.0 22.0 37.0 84-85 32.79438290451665 37.0 33.0 37.0 22.0 37.0 86-87 32.708405915390415 37.0 33.0 37.0 22.0 37.0 88-89 32.58478717870238 37.0 33.0 37.0 22.0 37.0 90-91 32.484113346407554 37.0 33.0 37.0 22.0 37.0 92-93 32.39781973576982 37.0 33.0 37.0 22.0 37.0 94-95 32.311152036265206 37.0 33.0 37.0 22.0 37.0 96-97 32.20004008544845 37.0 33.0 37.0 22.0 37.0 98-99 32.09558726284088 37.0 33.0 37.0 18.5 37.0 100-101 31.37248859784691 35.0 33.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.5349452224911424 2306 2 0.6631371176661283 2306 3 0.37690689648538367 2306 4 0.4292853952176472 2306 5 0.5227124835488013 2306 6 0.8600384111250321 2306 7 1.0382804907132765 2306 8 1.176620739412087 2306 9 1.306784913388121 2306 10-11 1.222211964410107 2306 12-13 1.1029796808652392 2306 14-15 1.1627063014038725 2306 16-17 1.1500657106974188 2306 18-19 1.1883071553457398 2306 20-21 1.13496269557956 2306 22-23 0.9330463210693907 2306 24-25 0.9855574197374111 2306 26-27 0.662567292170479 2306 28-29 0.7589155175735982 2306 30-31 0.7087635803831631 2306 32-33 0.7801039820448139 2306 34-35 0.8299416705485996 2306 36-37 0.7537109608992694 2306 38-39 0.6441567272062159 2306 40-41 0.5485242143207358 2306 42-43 0.6541706623074646 2306 44-45 0.5536071324667233 2306 46-47 0.6290852250987484 2306 48-49 0.7134769762623563 2306 50-51 0.686307360118775 2306 52-53 0.5382686717009406 2306 54-55 0.5245017325490622 2306 56-57 0.640694622217616 2306 58-59 0.5913709617497247 2306 60-61 0.48966320307059874 2306 62-63 0.48285279164834805 2306 64-65 0.3862047409585543 2306 66-67 0.40296962745456 2306 68-69 0.4333132062924818 2306 70-71 0.38075069192369426 2306 72-73 0.35886886238111515 2306 74-75 0.4173755883857311 2306 76-77 0.42067650924734323 2306 78-79 0.41798965548654365 2306 80-81 0.3598709679215233 2306 82-83 0.27157512336271594 2306 84-85 0.3216011763218276 2306 86-87 0.3513174948049169 2306 88-89 0.38111300459920017 2306 90-91 0.21910630414432575 2306 92-93 0.36603468279321305 2306 94-95 0.17293854220020677 2306 96-97 0.16081259269082437 2306 98-99 0.17434205655663249 2306 100-101 0.33531372784004887 2307 1 0.690790922494859 2307 2 0.8208804716694686 2307 3 0.5939563831646169 2307 4 0.7644066667231897 2307 5 0.7010490685425523 2307 6 1.0249700746784853 2307 7 1.1591434461889207 2307 8 1.252989470374125 2307 9 1.3556354217954336 2307 10-11 1.3385645135318 2307 12-13 1.2671894595179722 2307 14-15 1.251575850144711 2307 16-17 1.1916474889842164 2307 18-19 1.2941991938857171 2307 20-21 1.2937177199989094 2307 22-23 0.9926092566468796 2307 24-25 1.1467765032876684 2307 26-27 0.7715041145847579 2307 28-29 0.8726521246643415 2307 30-31 0.7673831860631211 2307 32-33 0.7436191712849336 2307 34-35 0.8421575212597929 2307 36-37 0.6882726409657423 2307 38-39 0.6909193547288126 2307 40-41 0.559023476080263 2307 42-43 0.6531217337537782 2307 44-45 0.6015142431110334 2307 46-47 0.6680604946535666 2307 48-49 0.7283034076331418 2307 50-51 0.7199026631774217 2307 52-53 0.6532681871415562 2307 54-55 0.6021882071528566 2307 56-57 0.7467865245380452 2307 58-59 0.7120414507800916 2307 60-61 0.5162241154429879 2307 62-63 0.48782291863005156 2307 64-65 0.5114733185175098 2307 66-67 0.51833466626411 2307 68-69 0.5289839599981434 2307 70-71 0.4836913294626868 2307 72-73 0.524932318215221 2307 74-75 0.5026591262067441 2307 76-77 0.482640932001388 2307 78-79 0.46289317659093854 2307 80-81 0.4839527821770204 2307 82-83 0.399259711306712 2307 84-85 0.35591768562658643 2307 86-87 0.2990432417932496 2307 88-89 0.3163432273146327 2307 90-91 0.3952453376695857 2307 92-93 0.3258962671150982 2307 94-95 0.33670685603397743 2307 96-97 0.29954404879313756 2307 98-99 0.2779081421773242 2307 100-101 0.17806552023472122 2201 1 0.8855379279153262 2201 2 0.8772835362328983 2201 3 0.536788686970965 2201 4 0.9076999613058234 2201 5 0.8291236306000087 2201 6 1.0450345967336219 2201 7 1.0674076868512898 2201 8 1.0853817486485156 2201 9 1.1068475522332335 2201 10-11 1.1422994990002948 2201 12-13 1.0115764362423008 2201 14-15 1.144492033143166 2201 16-17 1.1157212074358398 2201 18-19 1.0604776379351577 2201 20-21 1.1581466584372748 2201 22-23 1.00557754960969 2201 24-25 1.0646450870889197 2201 26-27 0.7917052477019624 2201 28-29 0.8566670040810038 2201 30-31 0.6899045474795287 2201 32-33 0.743879447884602 2201 34-35 0.848438572628929 2201 36-37 0.9000020927754804 2201 38-39 0.7872978735370353 2201 40-41 0.7549975741024681 2201 42-43 0.8592545556153013 2201 44-45 0.7420397325234163 2201 46-47 0.8056336889090332 2201 48-49 0.8233560835576128 2201 50-51 0.7154039958508491 2201 52-53 0.7226598411684435 2201 54-55 0.8082620260461439 2201 56-57 0.696001167058121 2201 58-59 0.70476970899945 2201 60-61 0.7398637099605381 2201 62-63 0.7406243561427246 2201 64-65 0.7352128993219083 2201 66-67 0.7467115420966266 2201 68-69 0.6499414760395297 2201 70-71 0.7381702857645749 2201 72-73 0.7287554373345984 2201 74-75 0.6243961059365191 2201 76-77 0.6547391243898346 2201 78-79 0.5914126353405109 2201 80-81 0.6531703026368021 2201 82-83 0.6882751801025364 2201 84-85 0.642813080844995 2201 86-87 0.6172785157062393 2201 88-89 0.7383182095356773 2201 90-91 0.6207442087417903 2201 92-93 0.6454228408289993 2201 94-95 0.653294925802939 2201 96-97 0.5155053860410916 2201 98-99 0.47098159703465825 2201 100-101 0.514234118581804 1101 1 0.9843192554329434 1101 2 0.8572583202423907 1101 3 0.809023878600069 1101 4 1.1012980060689088 1101 5 0.9776431040985614 1101 6 1.1035249276549095 1101 7 1.0001836180415324 1101 8 0.9242983269387466 1101 9 0.8400308997406825 1101 10-11 0.8845949371949544 1101 12-13 0.7319981885541154 1101 14-15 0.619318207326252 1101 16-17 0.6699312576155876 1101 18-19 0.7173566376983587 1101 20-21 0.7313263014755265 1101 22-23 0.3906921340371454 1101 24-25 0.6012935438800966 1101 26-27 0.38494587142314174 1101 28-29 0.4919238002196238 1101 30-31 0.42586756452874397 1101 32-33 0.44339636100765034 1101 34-35 0.43113331796738663 1101 36-37 0.45095699123951505 1101 38-39 0.45468002228728466 1101 40-41 0.36460619166157926 1101 42-43 0.4282610237540396 1101 44-45 0.41528653788932957 1101 46-47 0.4184584934926363 1101 48-49 0.4937552664661595 1101 50-51 0.3162154402512485 1101 52-53 0.3617494829229386 1101 54-55 0.48049349061782465 1101 56-57 0.4452611202779053 1101 58-59 0.36412663724155436 1101 60-61 0.3793384809817937 1101 62-63 0.2979952521913205 1101 64-65 0.3396503188278217 1101 66-67 0.23419521739688065 1101 68-69 0.2738234058722213 1101 70-71 0.27263464926033976 1101 72-73 0.3000387987338087 1101 74-75 0.3204310438998377 1101 76-77 0.31268372375186715 1101 78-79 0.25173295145521735 1101 80-81 0.24884349014807583 1101 82-83 0.24834536925258277 1101 84-85 0.26556465816204167 1101 86-87 0.2531975233602637 1101 88-89 0.34748428608492077 1101 90-91 0.3180194285911213 1101 92-93 0.19250423486894164 1101 94-95 0.189843429690562 1101 96-97 0.2575107537567263 1101 98-99 0.2136642350835416 1101 100-101 0.07165113226010433 2304 1 0.5489914939939631 2304 2 0.6131657344987183 2304 3 0.31547763336209655 2304 4 0.5126551268728576 2304 5 0.41106647690703113 2304 6 0.6186525133617593 2304 7 0.3725199703597042 2304 8 0.45003673334508676 2304 9 0.5283055915000716 2304 10-11 0.5116035437709812 2304 12-13 0.3845602807155615 2304 14-15 0.23407259468291386 2304 16-17 0.3004063900214291 2304 18-19 0.2356079544517229 2304 20-21 0.2691288746218845 2304 22-23 0.16393905990206292 2304 24-25 0.15138879349784418 2304 26-27 0.15466764980721592 2304 28-29 0.11350190472506938 2304 30-31 0.0283551751959763 2304 32-33 0.0729208687819849 2304 34-35 0.09042832710958493 2304 36-37 0.06414459271805839 2304 38-39 0.07746823222355204 2304 40-41 0.13002715729962944 2304 42-43 0.12747212484735115 2304 44-45 0.008380557237252617 2304 46-47 0.12271962946311277 2304 48-49 0.11333196381048083 2304 50-51 0.09285415085022919 2304 52-53 0.037664303671689936 2304 54-55 0.19405989301011317 2304 56-57 0.129417142979392 2304 58-59 0.13121786714069117 2304 60-61 0.11409965903187214 2304 62-63 0.1989852155126428 2304 64-65 0.1588025253866121 2304 66-67 0.1713080715009383 2304 68-69 0.13776019929567695 2304 70-71 0.13450640295812377 2304 72-73 0.10984867018515843 2304 74-75 0.16338617642159647 2304 76-77 0.1532245015398246 2304 78-79 0.19715854886779738 2304 80-81 0.08592190780542097 2304 82-83 0.121436497967494 2304 84-85 0.12751652610382536 2304 86-87 0.0910077155100808 2304 88-89 0.03011765517082665 2304 90-91 0.07010882860124212 2304 92-93 -0.015339160922110295 2304 94-95 0.026804543673385695 2304 96-97 0.031109409527850573 2304 98-99 -0.10816271259515275 2304 100-101 0.029999030447516617 2202 1 0.5951110690631225 2202 2 0.6321130586012522 2202 3 0.4264762652591152 2202 4 0.553028854483884 2202 5 0.6283430451477017 2202 6 0.5116907568600269 2202 7 0.39635327739819104 2202 8 0.44548872782825555 2202 9 0.3806567669258527 2202 10-11 0.3432900950456954 2202 12-13 0.18004496254255287 2202 14-15 0.07426092051257172 2202 16-17 0.06780615461703832 2202 18-19 0.16370337419839842 2202 20-21 0.1461439579919599 2202 22-23 -0.02364048971121946 2202 24-25 -0.004479232450307791 2202 26-27 0.04788373049984784 2202 28-29 0.041658627699717954 2202 30-31 -0.022003113254911 2202 32-33 0.04023757328700839 2202 34-35 0.07886509248292839 2202 36-37 0.08996863533513988 2202 38-39 0.019800517001797857 2202 40-41 0.06784555816907556 2202 42-43 0.1489397056183961 2202 44-45 0.04906097448783697 2202 46-47 -0.05025240192228608 2202 48-49 -0.06061739139522615 2202 50-51 -1.3365050141089796E-4 2202 52-53 0.025661189467705015 2202 54-55 0.05649088061205987 2202 56-57 0.17821890777209148 2202 58-59 0.07211652388286893 2202 60-61 -0.05595526403373441 2202 62-63 -0.002047211812957528 2202 64-65 0.01859857301714385 2202 66-67 0.033820546129497586 2202 68-69 0.051566299267591376 2202 70-71 0.008392130039858614 2202 72-73 -0.017103374959930306 2202 74-75 0.033066502064343695 2202 76-77 -0.06901514133763698 2202 78-79 -0.0739661386592374 2202 80-81 -0.08215095466668743 2202 82-83 0.01261436199646937 2202 84-85 -0.017012253332715943 2202 86-87 0.01844863654827833 2202 88-89 -0.06552336145414728 2202 90-91 -0.05116821479727918 2202 92-93 -0.11685111829022787 2202 94-95 -0.12917990057467676 2202 96-97 -0.11740072539939916 2202 98-99 -0.14410138299522401 2202 100-101 -0.3177697286872423 1102 1 0.6447925892459629 1102 2 0.6794336796540712 1102 3 0.6556726534162678 1102 4 0.7933902878491921 1102 5 0.7641820307534104 1102 6 0.4399097606915774 1102 7 0.42101184350036647 1102 8 0.3954320834271243 1102 9 0.29941929074956875 1102 10-11 0.3738430715178751 1102 12-13 0.1497627540379014 1102 14-15 -4.067284484605693E-4 1102 16-17 -0.04879123467300417 1102 18-19 0.08438585978392155 1102 20-21 0.03575394046183078 1102 22-23 0.03436204575917401 1102 24-25 0.0495338236761782 1102 26-27 -0.0082024935302627 1102 28-29 -0.0013652428263952743 1102 30-31 -0.013153660244853427 1102 32-33 0.027298257091011635 1102 34-35 0.05030851979987716 1102 36-37 0.11117378254616739 1102 38-39 -0.06498829257252225 1102 40-41 0.06928357012666453 1102 42-43 0.09180530249749808 1102 44-45 0.006878825161344082 1102 46-47 -0.03473875014922356 1102 48-49 -0.018536806897152758 1102 50-51 -0.07092530217312287 1102 52-53 -0.017574614431865143 1102 54-55 -0.007981205593928564 1102 56-57 -0.03012469656397343 1102 58-59 -0.010305052179951701 1102 60-61 -0.051301962983551164 1102 62-63 -0.045440408987865055 1102 64-65 -0.046305217390845144 1102 66-67 0.01241377233520069 1102 68-69 0.05410595679013852 1102 70-71 0.034278921974106424 1102 72-73 0.015148289371964552 1102 74-75 -0.019527115799384376 1102 76-77 -0.04685779701955539 1102 78-79 0.038709087510390816 1102 80-81 -0.021075707368140684 1102 82-83 0.04223672120357236 1102 84-85 -0.04884359387678927 1102 86-87 0.002775444251916781 1102 88-89 -0.01200685641799737 1102 90-91 -0.07447519567217142 1102 92-93 -0.08944976500867341 1102 94-95 -0.09306254574367046 1102 96-97 -0.1763526665545001 1102 98-99 -0.17497569512433842 1102 100-101 -0.21191995960228738 2305 1 0.4630859585687901 2305 2 0.5742269080112692 2305 3 0.5927297185423903 2305 4 0.7661418860533828 2305 5 0.6935078689900038 2305 6 0.24747600181506613 2305 7 0.17563465102681164 2305 8 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-1.9707343289817523 1213 76-77 -2.037370152004886 1213 78-79 -1.8870886151060766 1213 80-81 -1.8128978970165335 1213 82-83 -1.8854474610153957 1213 84-85 -1.9414949065678542 1213 86-87 -1.9885107867739045 1213 88-89 -2.063451917349937 1213 90-91 -2.070445369257836 1213 92-93 -2.0151915239394604 1213 94-95 -2.080486711743845 1213 96-97 -1.8724544436547887 1213 98-99 -1.7530994846367491 1213 100-101 -1.8840402183226708 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59393.0 3 17456.0 4 6543.0 5 6505.0 6 3186.0 7 8409.0 8 9080.0 9 8380.0 10 8305.0 11 10214.0 12 11624.0 13 11756.0 14 11350.0 15 11346.0 16 11690.0 17 11654.0 18 11861.0 19 12087.0 20 12037.0 21 12217.0 22 12001.0 23 12033.0 24 12179.0 25 12246.0 26 12501.0 27 13249.0 28 14986.0 29 17663.0 30 21498.0 31 27448.0 32 37750.0 33 57320.0 34 92954.0 35 169008.0 36 429221.0 37 1354928.0 38 623149.0 39 489.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 25.847836529579133 25.101944095018226 26.248640450864247 22.801578924538397 2 25.710511403927022 25.52569212573615 26.393444306186503 22.370352164150322 3 24.090777017167493 22.986416535678995 27.087312030556394 25.835494416597122 4 26.91095967931187 23.182557307529255 26.326678334176318 23.579804678982562 5 27.382956643172417 24.292822756020254 25.907729826609778 22.416490774197552 6 24.164803494993947 24.247645652876248 27.521952207436506 24.065598644693296 7 24.140300840737932 26.318344995750238 27.777537920844203 21.763816242667627 8 22.454794937859173 26.884910390193777 28.6813662720152 21.978928399931842 9 22.235956386159845 26.357305162989164 29.41935533947425 21.987383111376737 10-11 22.40484834232987 27.678667468177267 28.322470840529864 21.594013348963006 12-13 22.264488066290706 26.812823638322175 29.13550329283612 21.787185002551 14-15 22.251591021609492 27.02771971563437 28.602643872803863 22.11804538995227 16-17 22.228908607516075 26.820246907420813 28.657271422409053 22.29357306265406 18-19 22.235628769781275 26.21560710108418 28.826059606093814 22.722704523040736 20-21 22.644450339854536 26.37166121052949 28.245774601751716 22.73811384786426 22-23 22.687237358947705 26.545021108652563 27.93691827466483 22.830823257734902 24-25 22.71349825597134 26.368541527227684 27.716731184582695 23.201229032218276 26-27 22.61827267432746 26.79629962058846 27.70636746954189 22.879060235542195 28-29 22.59811238074116 26.704758274103384 28.01710680321932 22.680022541936136 30-31 22.132985743116212 26.524479528316824 28.218202195178403 23.124332533388557 32-33 22.353157495756097 26.377212162085556 27.98012564653454 23.289504695623812 34-35 22.53180242235943 26.53804099125083 27.530861048889854 23.399295537499885 36-37 22.86923857412842 26.166190537919892 27.57035610401602 23.394214783935666 38-39 22.866158334524567 26.560587256825773 27.633835167834075 22.939419240815585 40-41 22.97617480798322 26.530504142557938 27.672092044566416 22.821229004892427 42-43 23.077016805678284 26.479060689906376 27.503439751587305 22.940482752828036 44-45 23.122214829033226 26.515428661285522 27.427411735503465 22.934944774177783 46-47 22.961209882806358 26.920311146663188 27.2621239098044 22.856355060726052 48-49 22.809791692416084 26.970031493738038 27.34415260958208 22.876024204263796 50-51 22.82874473928336 26.77498272891437 27.880104797682613 22.51616773411966 52-53 22.851369976410616 26.72481233205419 27.944802236011807 22.479015455523392 54-55 22.97254130470316 26.356759939596653 28.22519191255488 22.445506843145303 56-57 22.713205851359557 26.68859892113974 27.97405497371736 22.624140253783338 58-59 22.843304067622928 26.448434983565043 27.841044403456785 22.867216545355245 60-61 22.583661354135533 26.61260032203167 27.721735642456757 23.08200268137604 62-63 22.78313249095478 26.57614402137695 27.652159637996103 22.98856384967216 64-65 23.071661017026283 26.49723325541342 27.454765631615274 22.976340095945016 66-67 22.593730672988386 26.847172658836033 27.5550664910152 23.004030177160377 68-69 22.79504519989721 26.485740296736022 27.57365209350528 23.14556240986149 70-71 22.78149283751723 26.51860854336251 27.771697669799973 22.92820094932029 72-73 22.826988692513343 26.39662933913098 27.83789178308766 22.93849018526802 74-75 23.125879579706783 26.725016522850947 27.60306016528378 22.54604373215849 76-77 23.10192905801414 27.307929325579888 27.1733824423226 22.41675917408338 78-79 22.615649426324232 27.145144276326587 27.18993958484696 23.049266712502213 80-81 22.384395640007952 27.009691453542505 27.756650593609862 22.849262312839684 82-83 22.182228183137923 27.274537058028088 27.53419766610974 23.009037092724256 84-85 22.17063565449054 27.195656674617535 27.688358051009278 22.945349619882652 86-87 22.636825664414854 27.30052519951584 27.396713443713054 22.665935692356253 88-89 22.563086325781974 27.471379881974574 27.507983465972565 22.45755032627089 90-91 22.453509208448217 27.239623776684287 27.71871141206607 22.588155602801425 92-93 22.499549343878822 27.21862193990677 27.43071070705304 22.851118009161365 94-95 22.361686644093076 27.232357725348084 27.807680297892063 22.598275332666777 96-97 22.18854554462269 27.322189959650835 27.680560233122726 22.808704262603747 98-99 22.420787121316675 26.90907666346598 27.70768728352912 22.962448931688222 100-101 22.3421021616591 27.184494901670725 27.764399884581465 22.70900305208871 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 45769.0 1 26209.0 2 7574.0 3 8716.0 4 8112.0 5 6202.5 6 4372.5 7 3275.5 8 2587.0 9 1992.0 10 1541.0 11 1230.0 12 1004.5 13 861.5 14 765.5 15 669.0 16 599.5 17 604.0 18 622.5 19 639.0 20 714.0 21 874.0 22 1253.5 23 1675.5 24 2376.5 25 3947.0 26 5080.0 27 4983.0 28 5716.5 29 6797.0 30 7401.0 31 8209.0 32 9461.0 33 11528.0 34 18344.5 35 26226.5 36 37899.5 37 61229.0 38 84979.5 39 97643.5 40 110857.5 41 143282.0 42 175266.5 43 193559.5 44 210891.0 45 230717.5 46 245414.5 47 249847.5 48 224598.0 49 171703.0 50 128929.0 51 100438.5 52 76863.0 53 66728.0 54 63194.5 55 50130.5 56 36273.0 57 28430.5 58 24801.5 59 22623.5 60 20137.0 61 17629.5 62 14749.0 63 12345.0 64 10520.0 65 8769.0 66 7303.5 67 6151.0 68 5059.0 69 4147.5 70 3331.5 71 2701.5 72 2222.5 73 1754.5 74 1364.0 75 1072.0 76 875.5 77 715.5 78 568.0 79 462.5 80 403.5 81 348.5 82 294.5 83 246.0 84 200.5 85 161.0 86 145.5 87 142.0 88 120.0 89 95.5 90 71.0 91 52.5 92 45.5 93 35.0 94 26.0 95 21.5 96 17.5 97 14.5 98 9.5 99 12.5 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.9986358981722545 2 1.8628240056730514 3 1.5977499247413811 4 1.509643809459032 5 1.5069987366628501 6 2.0461842305798124 7 2.2676460993363388 8 2.4230126371501735 9 2.634681438768454 10-11 2.5158106077495597 12-13 2.700194223916748 14-15 2.877366867818155 16-17 2.802911217501817 18-19 2.8390133122735155 20-21 2.907627760164952 22-23 2.9799106721129975 24-25 2.9888692817619713 26-27 3.0835093566301266 28-29 3.0267977363215097 30-31 3.0663478724168027 32-33 3.0694652796408746 34-35 3.078565589618215 36-37 3.078581334099145 38-39 3.0757158385699475 40-41 3.1020091217224715 42-43 3.0889254580699284 44-45 3.0798723815353766 46-47 2.98482295016305 48-49 3.0597037014644886 50-51 3.073149488178414 52-53 3.094499004319026 54-55 3.098608313841666 56-57 3.0924994552409597 58-59 3.080407693886985 60-61 3.1271845467289894 62-63 3.1333406387724847 64-65 3.12579903240718 66-67 3.1346789196515052 68-69 3.134647430689646 70-71 3.1236735274816763 72-73 3.133907440085952 74-75 3.1385992954029893 76-77 3.1437477406669863 78-79 3.1331831939631885 80-81 3.1250432973225566 82-83 3.1176433912856187 84-85 3.1143527947713205 86-87 3.1317819351604483 88-89 3.133608294948289 90-91 3.121784189770118 92-93 3.1377333489518584 94-95 3.139528219777839 96-97 3.135292954407762 98-99 3.1429605166205037 100-101 3.1474319492045257 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3175716.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.756878756192815 #Duplication Level Percentage of deduplicated Percentage of total 1 89.61021734449578 23.9768972080276 2 6.429668087707451 3.4407569893070074 3 1.0923226998636193 0.8768143812866417 4 0.4865814100531661 0.5207759917523962 5 0.2599114778755719 0.34772099504297843 6 0.1531254606395983 0.24582956308919443 7 0.09311267892593396 0.17439832624798673 8 0.07199948481249915 0.15411851885091088 9 0.051278661737978834 0.12348512414126309 >10 0.36318656027462287 1.9296898742482274 >50 0.14329189885186644 3.1209132858969406 >100 1.2407015492771294 62.75745185896854 >500 0.0038811985762395966 0.6521924221244293 >1k 4.864421899569407E-4 0.1429461768521413 >5k 1.1752236186430904E-4 0.17169142467994256 >10k+ 1.1752235666108564E-4 1.3643178594837777 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 43387 1.366211588189876 No Hit CATGGTTATATTTAGTTTTAGATGGAGTTTACCACCCACTTAGTGCTGCA 5460 0.1719297317518317 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0037786754231171806 0.0 0.0 2 0.010706247032165345 0.0 0.0 3 0.031142583278857428 0.0 0.0 4 0.08533508663872966 0.0 0.0 5 0.12409799868754007 0.0 0.0 6 0.17425991492942064 0.0 0.0 7 0.22775966112838805 0.0 0.0 8 0.24227607254552988 0.0 0.0 9 0.24725132851930084 0.0 0.0 10-11 0.25101425946148836 0.0 0.0 12-13 0.2578945976277476 0.0 0.0 14-15 0.27280462106813075 0.0 1.574448092965492E-5 16-17 0.29350861349062696 0.0 6.297792371861968E-5 18-19 0.3201482752236031 0.0 9.446688557792951E-5 20-21 0.362311995153219 0.0 9.446688557792951E-5 22-23 0.4353034087430992 0.0 9.446688557792951E-5 24-25 0.5401773962155306 0.0 1.1021136650758443E-4 26-27 0.6766977903565684 0.0 1.2595584743723936E-4 28-29 0.8336230317824389 0.0 1.2595584743723936E-4 30-31 0.9472037172089696 0.0 1.2595584743723936E-4 32-33 1.0071901895509547 0.0 1.2595584743723936E-4 34-35 1.0668775167552766 0.0 1.2595584743723936E-4 36-37 1.1135284137498442 0.0 1.5744480929654917E-4 38-39 1.1436790947301332 0.0 1.8893377115585902E-4 40-41 1.1650286108707455 0.0 1.8893377115585902E-4 42-43 1.188314698165705 0.0 1.8893377115585902E-4 44-45 1.2139939465619722 0.0 2.0467825208551394E-4 46-47 1.2378468351703993 0.0 2.2042273301516887E-4 48-49 1.2589286951352072 0.0 2.361672139448238E-4 50-51 1.2836632746756953 0.0 2.6765617580413363E-4 52-53 1.3135935329229693 0.0 2.8340065673378856E-4 54-55 1.34823139096821 0.0 3.1488961859309835E-4 56-57 1.3791063180712633 0.0 3.1488961859309835E-4 58-59 1.4063285255986366 0.0 3.1488961859309835E-4 60-61 1.4310158716963355 0.0 3.1488961859309835E-4 62-63 1.455041949594989 0.0 3.1488961859309835E-4 64-65 1.490167886549049 0.0 3.1488961859309835E-4 66-67 1.5346775341371837 0.0 3.463785804524082E-4 68-69 1.5917827664690418 0.0 3.463785804524082E-4 70-71 1.6531547531328368 0.0 3.7786754231171804E-4 72-73 1.6989869371190622 0.0 4.093565041710279E-4 74-75 1.7311844006202066 0.0 4.251009851006828E-4 76-77 1.7586112863996655 0.0 4.4084546603033773E-4 78-79 1.7834403328257313 0.0 4.4084546603033773E-4 80-81 1.8044119814240316 0.0 4.4084546603033773E-4 82-83 1.8237934374484368 0.0 4.4084546603033773E-4 84-85 1.847599092614075 0.0 4.723344278896476E-4 86-87 1.8745064105228553 0.0 4.723344278896476E-4 88-89 1.9034605109524907 0.0 5.038233897489574E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGCAT 435 0.0 32.57676 1 ATATTTA 3075 0.0 31.170044 8 CTAGTAA 1620 0.0 30.661572 84-85 CGTGGTT 330 0.0 30.059464 1 CACGACT 1680 0.0 29.709675 62-63 CGACTCT 1740 0.0 29.5019 64-65 ACGACTC 1720 0.0 29.295118 62-63 AGTTTAC 1705 0.0 29.261438 26-27 CATCTAG 1715 0.0 29.237661 80-81 CTTAGTG 1705 0.0 29.122808 38-39 TCTAGTA 1745 0.0 28.873775 82-83 ATCTAGT 1750 0.0 28.383856 82-83 CTAGACG 670 0.0 28.33125 9 CAACACG 1790 0.0 28.145153 58-59 TGCACTA 1810 0.0 27.83099 46-47 ACCCACT 1835 0.0 27.19041 34-35 GCTAGAC 665 0.0 27.113968 8 GCAACAC 1895 0.0 26.836468 58-59 CACTTAG 1855 0.0 26.768082 36-37 TTACCAC 1885 0.0 26.594791 28-29 >>END_MODULE