FastQCFastQC Report
Thu 11 Sep 2014
lane8_Undetermined_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane8_Undetermined_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3175716
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN433871.366211588189876No Hit
CATGGTTATATTTAGTTTTAGATGGAGTTTACCACCCACTTAGTGCTGCA54600.1719297317518317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCAT4350.032.576761
ATATTTA30750.031.1700448
CTAGTAA16200.030.66157284-85
CGTGGTT3300.030.0594641
CACGACT16800.029.70967562-63
CGACTCT17400.029.501964-65
ACGACTC17200.029.29511862-63
AGTTTAC17050.029.26143826-27
CATCTAG17150.029.23766180-81
CTTAGTG17050.029.12280838-39
TCTAGTA17450.028.87377582-83
ATCTAGT17500.028.38385682-83
CTAGACG6700.028.331259
CAACACG17900.028.14515358-59
TGCACTA18100.027.8309946-47
ACCCACT18350.027.1904134-35
GCTAGAC6650.027.1139688
GCAACAC18950.026.83646858-59
CACTTAG18550.026.76808236-37
TTACCAC18850.026.59479128-29
Produced by FastQC (version 0.11.2)