FastQCFastQC Report
Thu 11 Sep 2014
lane8_Undetermined_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane8_Undetermined_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3175716
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTAT5500.051.300421
CGGGACG4800.050.9441761
GCACGAC5850.050.610033
GGGGGGT10050.048.1951751
GGGGTAC4500.047.025391
GGGGGTT11400.044.550371
GGGCGCT9300.044.4971431
CGGGTAT3000.042.3228531
GGGACGC11900.041.4681632
GGGGGGC15450.040.7857741
GGGGGAT10550.040.5621831
GGGGGGA13000.040.514181
GGGGTGC6550.040.2049141
GGGGACT9750.039.5495571
GGGCACG6900.039.528591
GGGGGAC9850.039.1480371
GGGGTAG6800.038.726791
GGGCTAC6100.038.54541
GGGGATC8850.038.2579461
GCCCCTA6050.038.0638922
Produced by FastQC (version 0.11.2)