FastQCFastQC Report
Thu 11 Sep 2014
LNvs_CT12_2_TGACCA_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLNvs_CT12_2_TGACCA_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22762210
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGTTATATTTAGTTTTAGATGGAGTTTACCACCCACTTAGTGCTGCA3062221.3453087375962176No Hit
GCATGAGTTGTTAATTTGGGCACCGTAACATTACGTTTGGTTCATCCCAC637970.28027594860077293No Hit
TTCGCATTGTTAATCATACAAGTGCATATAATAAACACAAAAATCAATGA559300.24571427818300598No Hit
GCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT550890.24201955785488316No Hit
CATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTATAA515090.22629173529283847No Hit
CATTCGATTATTTGCTACTACCACCAAGATCTGTACCAATGGCAGCTCCA506620.22257065548556138No Hit
CCACTTCAGTCCTCCAAGGTCTCATTCGATTATTTGCTACTACCACCAAG500150.21972822498342648No Hit
CGTGGTTATATTTAGTTTTAGATGGAGTTTACCACCCACTTAGTGCTGCA477360.2097160161513315No Hit
TATGGTTATATTTAGTTTTAGATGGAGTTTACCACCCACTTAGTGCTGCA473190.2078840323501101No Hit
GCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG415750.1826492243064272No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA411220.1806590836302802No Hit
ATTTGCTACTACCACCAAGATCTGTACCAATGGCAGCTCCATGCAGGCTT395790.1738803042411084No Hit
GCTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAA338440.14868503541615685No Hit
ATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAGCCCAAAAG333400.1464708391671986No Hit
GTCCGCACTGAGCTGGCCTTGGGACACCTCCGTTATTATTTGAGAGATGT314180.13802701934478243No Hit
ACCACCAAGATCTGTACCAATGGCAGCTCCATGCAGGCTTACGCCAAACA306250.13454317484989375No Hit
TCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAGCCCAAAAGCT300420.13198191212540433No Hit
GTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAG287140.1261476807392604No Hit
GTGGTTTCGCTAGATAGTAGATAGGGACAGTAGGAATCTCGTTAATCCAT250240.10993660105938746No Hit
GCATGGGTTGTTAATTTGGGCACCGTAACATTACGTTTGGTTCATCCCAC241650.10616280229380187No Hit
CCAAGGTCTCATTCGATTATTTGCTACTACCACCAAGATCTGTACCAATG237200.10420780758986056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATA813800.055.142224
TGGTTAT814350.053.583513
GTTATAT865200.052.5540125
TATTTAG979800.049.7318659
TTATATT953250.048.4318736
CATGGTT635400.047.677051
CGTGGTT108800.047.2497371
TATATTT1044000.046.4377487
GTGGTTA129500.045.8919262
ATGGTTA832250.042.976512
ATATTTA1163400.041.8759048
TAATACG78750.041.665198
CGCATTG164450.040.2402953
TCGCATT153100.038.910812
TAACGTT681800.038.30806494-95
TTCGCAT152350.037.793591
TTCGCGT53100.037.1876371
AATACGC91050.036.192599
TTAACGT728450.036.0374594-95
GCATTGT219350.035.6225054
Produced by FastQC (version 0.11.2)