FastQCFastQC Report
Thu 11 Sep 2014
LNvs_CT12_2_TGACCA_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLNvs_CT12_2_TGACCA_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22762210
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTG602510.26469749642060236No Hit
GGGGGTTCGAAGGCGATCAGATACCGCCCTAGTTCTAACCATAAACGATG367240.16133758541020402No Hit
GGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTA339870.1493132696693335No Hit
GGGGGGCTAGAAGAGCATGACATATACTGTTGTGTCGATATTTTCTCCTC314100.13799187337257673No Hit
GGGGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCG256690.11277024506847093No Hit
GGGGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGT248850.10932593979231366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGT206600.067.326981
GGGGTAT168550.065.039271
GGGGGTT295550.060.912931
CGGGTAT61050.059.0240061
GGGGTAC91800.056.7043761
GCGCTCT147250.054.6140173
GGGGGGA354650.054.2473341
GATAATT167300.053.775763
GGGGACG159900.052.5331231
GGGGGAG315850.050.9324571
GGGGGGC378000.050.7705761
GGGGGAC259250.050.628231
GGGGGAT278500.050.320791
GGGGATC177600.049.3853951
GCGCTAA52950.048.981173
GGCGCTC137600.048.539062
CGGGGTA44000.048.4027941
GGGGGGG336400.048.075461
CGGGGGG103400.046.3431051
GGGCTAT74400.046.1966481
Produced by FastQC (version 0.11.2)