FastQCFastQC Report
Thu 11 Sep 2014
LNvs_CT0_2_CGATGT_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLNvs_CT0_2_CGATGT_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25509895
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGTTATATTTAGTTTTAGATGGAGTTTACCACCCACTTAGTGCTGCA2158730.8462324129519152No Hit
CATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTATAA451770.17709598569496268No Hit
GCATGAGTTGTTAATTTGGGCACCGTAACATTACGTTTGGTTCATCCCAC450830.1767275012304049No Hit
GCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT435640.1707729490850511No Hit
TTCGCATTGTTAATCATACAAGTGCATATAATAAACACAAAAATCAATGA426440.1671665053893793No Hit
TATGGTTATATTTAGTTTTAGATGGAGTTTACCACCCACTTAGTGCTGCA381150.1494126102831862No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA374590.14684105912627238No Hit
CATTCGATTATTTGCTACTACCACCAAGATCTGTACCAATGGCAGCTCCA344580.1350769965928907No Hit
CGTGGTTATATTTAGTTTTAGATGGAGTTTACCACCCACTTAGTGCTGCA319500.12524551747468973No Hit
GCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG299200.11728782105923995No Hit
CCACTTCAGTCCTCCAAGGTCTCATTCGATTATTTGCTACTACCACCAAG297320.11655085213012441No Hit
ATTTGCTACTACCACCAAGATCTGTACCAATGGCAGCTCCATGCAGGCTT282730.11083150283448834No Hit
GTCCGCACTGAGCTGGCCTTGGGACACCTCCGTTATTATTTGAGAGATGT257010.10074914067658844No Hit
GCTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAA255730.10024737459719063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATA622800.052.6150934
GTTATAT653700.050.711025
TGGTTAT647650.049.291323
TATTTAG789900.045.4079489
CATGGTT502350.044.1692771
TTATATT763400.044.101986
TATATTT847050.042.014857
ATGGTTA674950.039.1836282
TAACGTT514050.037.20577694-95
ATATTTA1022000.035.222278
TTAACGT545000.035.1582894-95
CGTGGTT108750.032.826651
TAATACG79950.032.7883578
GTGGTTA127850.032.7871062
TTCGCAT137500.031.8321671
CTAGTAA598150.031.73559884-85
CATCTAG611000.031.38959980-81
CATTAAC604800.031.37944692-93
ATCTAGT606150.031.32082682-83
AGTTTAC612300.031.29603226-27
Produced by FastQC (version 0.11.2)