FastQCFastQC Report
Thu 11 Sep 2014
LNvs_CT0_2_CGATGT_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLNvs_CT0_2_CGATGT_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25509895
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTG658990.25832720989247504No Hit
GGGGGTTCGAAGGCGATCAGATACCGCCCTAGTTCTAACCATAAACGATG303910.11913416342952411No Hit
GGGGGGCTAGAAGAGCATGACATATACTGTTGTGTCGATATTTTCTCCTC284580.11155671162111801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTAT184500.057.5299761
GGGGGGT222550.056.9208561
GGGGTAC103650.052.4177971
GGGGGTT290550.052.2862741
GGGGACG198350.050.1099131
GCGCTCT217850.049.3201453
GGGGGGC339200.048.1082761
GATAATT213850.046.6443823
GGGGGGA333450.046.5715031
GGGGGAT265100.045.5255131
CGGGTAT74450.045.4587141
GGGGTGC172950.044.6341551
GGGGGAC227200.044.6256331
GGGGGAG292100.044.262831
GGGCCTA78700.043.668211
GGGGATC197950.042.6230661
GGGCGCT239000.042.104141
GGCGCTC219650.041.737752
GGGGGGG307600.040.9507031
GGGGCTC168100.040.3513531
Produced by FastQC (version 0.11.2)